SitesBLAST
Comparing WP_066330061.1 NCBI__GCF_900100165.1:WP_066330061.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7dkmA Phgdh covalently linked to oridonin (see paper)
37% identity, 99% coverage: 2:315/316 of query aligns to 4:303/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V73), A102 (≠ V101), G148 (= G153), R151 (= R156), I152 (= I157), Y170 (≠ A175), D171 (= D176), P172 (≠ S177), I173 (≠ F178), H202 (= H216), T203 (≠ V217), P204 (= P218), T209 (≠ N221), C230 (= C242), A231 (= A243), R232 (= R244), H279 (= H291), G281 (= G293)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ S12), K17 (≠ L15), I18 (≠ A16), E293 (≠ T305)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
37% identity, 99% coverage: 2:315/316 of query aligns to 3:302/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V152), G147 (= G153), L148 (≠ I154), G149 (= G155), R150 (= R156), I151 (= I157), G152 (= G158), D170 (= D176), H201 (= H216), T202 (≠ V217), P203 (= P218)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 99% coverage: 2:314/316 of query aligns to 3:301/301 of 6rj5A
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
37% identity, 99% coverage: 2:315/316 of query aligns to 3:302/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
37% identity, 99% coverage: 2:315/316 of query aligns to 3:302/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 99% coverage: 2:315/316 of query aligns to 4:303/305 of 6plfA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 98% coverage: 2:312/316 of query aligns to 2:298/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ S97), A100 (≠ V101), R149 (= R156), I150 (= I157), Y168 (≠ A175), D169 (= D176), P170 (≠ S177), I171 (≠ F178), H200 (= H216), T201 (≠ V217), P202 (= P218), T207 (≠ N221), C228 (= C242), A229 (= A243), R230 (= R244), H277 (= H291), G279 (= G293)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
37% identity, 99% coverage: 2:315/316 of query aligns to 8:307/533 of O43175
- T78 (≠ V73) binding NAD(+)
- R135 (≠ K135) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 156:157) binding NAD(+)
- D175 (= D176) binding NAD(+)
- T207 (≠ V217) binding NAD(+)
- CAR 234:236 (= CAR 242:244) binding NAD(+)
- D260 (= D268) binding NAD(+)
- V261 (= V269) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGA 291:294) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
38% identity, 98% coverage: 2:310/316 of query aligns to 2:296/297 of 6rj3A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
37% identity, 99% coverage: 3:315/316 of query aligns to 1:299/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G155), I148 (= I157), Y166 (≠ A175), D167 (= D176), P168 (≠ S177), I169 (≠ F178), I170 (= I179), H198 (= H216), T199 (≠ V217), L208 (≠ I224), R228 (= R244)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
39% identity, 100% coverage: 1:316/316 of query aligns to 1:303/304 of 1wwkA
- active site: S96 (= S97), R230 (= R244), D254 (= D268), E259 (= E273), H278 (= H291)
- binding nicotinamide-adenine-dinucleotide: V100 (= V101), G146 (= G153), F147 (≠ I154), G148 (= G155), R149 (= R156), I150 (= I157), Y168 (≠ A175), D169 (= D176), P170 (≠ S177), V201 (= V217), P202 (= P218), T207 (≠ N221), T228 (≠ C242), S229 (≠ A243), D254 (= D268), H278 (= H291), G280 (= G293)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
36% identity, 99% coverage: 2:314/316 of query aligns to 2:292/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R156), Y160 (≠ A175), D161 (= D176), P162 (≠ S177), I164 (= I179), L179 (= L203), T193 (≠ V217), P194 (= P218), S198 (≠ Q220), L202 (≠ I224)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
38% identity, 84% coverage: 46:311/316 of query aligns to 45:297/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
38% identity, 84% coverage: 46:311/316 of query aligns to 46:298/525 of 3ddnB
2eklA Structure of st1218 protein from sulfolobus tokodaii
35% identity, 98% coverage: 1:310/316 of query aligns to 5:300/312 of 2eklA