SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066551960.1 NCBI__GCF_001636925.1:WP_066551960.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
39% identity, 96% coverage: 1:425/444 of query aligns to 1:459/485 of Q8DLI5

query
sites
Q8DLI5

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R6 (= R6) binding L-glutamate
Y192 (= Y184) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
39% identity, 95% coverage: 2:425/444 of query aligns to 1:458/484 of 2cfoA

query
sites
2cfoA

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active site: S9 (= S10), L239 (= L232), K250 (= K243)
binding glutamic acid: R5 (= R6), Y191 (= Y184), R209 (= R202), H213 (= H206)

P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 98% coverage: 1:437/444 of query aligns to 1:456/471 of P04805

query
sites
P04805

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L

A

K

D

E

M

M

A

A

Q

Q

I

S

V

C

R

R

G

Y

P

F

I

Y

Q

E

P

D

V

F

I

A

E

E

-

F

D

D

A

A

T

D

F

A

I

A

E

K

T

K

A

H

L

L

R

R

L

P

L

V

P

A

-

R

-

Q

-

P

-

L

E

E

V

V

I

V

D

R

E

D

N

K

-

L

-

A

A

A

W

I

S

T

A

D

W

W

T

T

S

A

-

E

-

N

-

V

-

H

A

A

V

I

K

Q

A

A

-

T

-

A

-

D

-

E

-

L

E

E

T

V

G

G

A

M

K

-

G

G

K

K

A

-

L

V

F

G

M

M

P

P

L

L

R

R

L

V

A

A

L

V

T

T

G

G

Q

A

Q

G

H

Q

G

S

P

P

D

A

M

L

A

D

A

V

M

T

A

V

P

H

L

A

I

I

G

G

R

K

-

T

E

R

T

S

I

I

Q

E

R

R

C98 (≠ A97) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (≠ E99) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (≠ A124) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (≠ E126) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (≠ T128) mutation to Q: No change in activity or in zinc content.
H131 (≠ Y135) mutation to Q: No change in activity or in zinc content.
H132 (≠ E136) mutation to Q: No change in activity or in zinc content.
C138 (≠ P142) mutation to S: No change in activity or in zinc content.
S239 (= S242) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
36% identity, 98% coverage: 1:437/444 of query aligns to 1:456/468 of 8i9iA

query
sites
8i9iA

M

M

S

K

V

I

K

K

V

T

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

K

Y

L

L

H

H

V

V

G

G

N

G

V

A

R

R

T

T

A

A

L

L

V

Y

N

S

W

W

M

L

F

F

A

A

K

R

G

N

Q

H

G

G

S

E

F

F

V

V

L

L

R

R

I

I

D

E

D

D

T

T

D

D

L

L

A

E

R

R

S

S

T

T

P

P

E

E

F

A

E

I

Q

E

G

A

I

I

E

M

D

D

D

G

L

M

T

N

W

W

L

L

G

S

M

L

P

E

W

W

D

D

E

E

-

G

R

P

Y

Y

N

Y

Q

Q

S

T

K

K

R

R

F

F

D

D

R

R

Y

Y

E

N

E

A

A

V

A

I

A

D

S

Q

L

M

K

L

A

E

S

E

G

G

R

T

L

A

Y

Y

P

K

A

C

Y

Y

E
x
C

T

S

A

K

D

E

E

R

L

L

D

E

R

A

R

L

R

R

K

E

V

E

Q

Q

L

M

S

A

R

K

G

G

L

E

P

K

P

P

I

R

Y
|
Y

D

D

R

G

A

R

A
x
C

L

R

E

H

L

S

T

H

E

E

E

-

Q

-

K

-

A

-

Y

H

E

H

A

A

E

D

G

D

R

E

R

P

P

C

H

V

W

V

R

R

F

F

K

A

L

N

D

P

G

Q

K

E

-

G

R

S

V

V

A

V

W

F

E

D

D

D

L

Q

A

I

R

R

G

G

H

P

A

I

E

E

V

F

D

S

T

N

A

Q

S

E

M

L

S

D

D

D

P

L

V

I

L

I

I

R

R

R

E

T

D

D

G

G

L

S

F

P

L

T

Y

Y

T

N

L

F

P

C

S

V

V

V

I

V

D

D

I

W

D

D

M

M

G

E

I

I

T

T

H

H

I

V

I

I

R
|
R

G

G

E

E

D

D

H
|
H

V

I

T

N

N

N

T

T

G

P

A

R

Q

Q

I

I

E

N

I

I

F

L

E

K

A

A

L

L

G

K

A

A

T

P

V

V

P

P

G

V

F

Y

A

A

H

H

M

V

P

S

L

M

L

I

V

N

G

G

A

D

D

D

G

G

A

E

A

K

L

L

S

S

K

K

R

R

L

H

G

G

S

A

L

V

S

S

I

V

S

M

D

Q

M

Y

R

R

D

D

Q

D

G

G

Y

Y

E

L

P

P

I

E

A

A

I

L

T

L

S

N

H

Y

L

L

G

V

R

R

I

L

G

G

T

W

S

S

D

H

P

G

L

D

E

Q

V

E

G

I

A

F

S

T

V

R

E

E

A

E

L

M

G

I

Q

K

S

Y

F

F

A

T

F

L

S

N

K

A

M

V

G

S

R

K

S

S

P

A

A

S

R

A

Y

F

D

N

T

T

A

D

D

K

L

L

D

L

R

W

L

L

N

N

A

H

Q

H

A

Y

L

I

H

N

V

A

M

L

D

P

Y

P

E

E

T

Y

A

V

Q

A

P

T

R

H

L

L

Q

Q

-

W

-

H

-

I

-

A

-

Q

-

E

A

N

L

I

G

D

A

T

D

R

L

N

G

G

E

P

A

Q

F

L

W

A

N

D

T

L

V

V

R

K

G

L

N

L

L

G

T

E

K

R

F

C

A

K

D

T

V

L

A

K

D

E

M

M

A

A

Q

Q

I

S

V

C

R

R

G

Y

P

F

I

Y

Q

E

P

D

V

F

I

A

E

E

-

F

D

D

A

A

T

D

F

A

I

A

E

K

T

K

A

H

L

L

R

R

L

P

L

V

P

A

-

R

-

Q

-

P

-

L

E

E

V

V

I

V

D

R

E

D

N

K

-

L

-

A

A

A

W

I

S

T

A

D

W

W

T

T

S

A

-

E

-

N

-

V

-

H

A

A

V

I

K

Q

A

A

-

T

-

A

-

D

-

E

-

L

E

E

T

V

G

G

A

M

K

-

G

G

K

K

A

-

L

V

F

G

M

M

P

P

L

L

R

R

L

V

A

A

L

V

T

T

G

G

Q

A

Q

G

H

Q

G

S

P

P

D

A

M

L

A

D

A

V

M

T

A

V

P

H

L

A

I

I

G

G

R

K

-

T

E

R

T

S

I

I

Q

E

R

R

binding glutamic acid: R6 (= R6), A8 (= A8), P9 (= P9), S10 (= S10), R199 (= R202), H203 (= H206)
binding zinc ion: C100 (≠ E99), Y121 (= Y120), C125 (≠ A124)

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
39% identity, 75% coverage: 3:334/444 of query aligns to 3:320/380 of 4g6zA

query
sites
4g6zA

V

V

K

R

V

T

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

K

F

L

I

H

H

V

L

G

G

N

N

V

I

R

R

T

S

A

A

L

L

V

Y

N

P

W

W

M

A

F

F

A

A

K

R

G

K

Q

M

G

K

G

G

S

T

F

F

V

V

L

L

R

R

I

I

D
x
E

D

D

T

T

D

D

L

V

A

E

R

R

S

S

T

S

P

Q

E

E

F

A

E

V

Q

D

G

A

I

I

E

L

D

E

D

G

L

M

T

A

W

W

L

L

G

G

M

L

P

D

W

Y

D

D

E

E

-

G

R

P

Y

Y

N

Y

Q

Q

S

M

K

Q

R

R

F

M

D

D

R

R

Y

Y

E

R

E

E

A

V

A

L

A

A

S

Q

L

M

K

Q

A

E

S

K

G

G

R

L

L

V

Y

Y

P

P

A

C

Y

Y

E

M

T

R

A

Y

D

D

E

G

L

T

D

W

R

R

R

P

R

E

K

P

V

G

Q

K

L

V

S

L

R

P

G

E

L

P

P

P

P

-

I

-

Y

-

D

-

R

-

A

-

L

-

E

-

L

-

T

-

E

-

Q

-

K

-

A

-

Y

-

E

-

A

-

E

A

G

G

R

V

R

A

P

P

H

V

W

L

R

R

F

F

K

R

-

N

-

P

L

L

D

T

G

G

K

T

R

-

V

V

A

A

W

W

E

D

D

D

L

A

A

V

R

K

G

G

H

R

A

V

E

E

V

I

D

S

T

N

A

E

S

E

M

L

S

D

D

D

P

L

V

V

L

V

I

A

R

R

E

P

D

D

G

G

L

T

F

P

L

M

Y

Y

T

N

L

F

P

C

S

V

V

V

I

V

D

D

I

L

D

D

M

M

G

G

I

I

T

T

H

H

I

V

I

I

R
|
R

G

G

E

D

D

D

H
|
H

V

V

T

N

N

N

T

T

G

P

A

R

Q

Q

I

I

E

N

I

I

F

L

E

R

A

A

L

L

G

G

A

G

T

E

V

V

P

P

G

V

F

Y

A

A

H

H

M

L

P

P

L
x
T

L

V

V

L

G

N

A

E

D

Q

G

G

A

E

A

K

L

M

S

S

K
|
K

R

R

L

H

G

G

S

A

L

M

S

S

I

V

S

M

D

G

M

Y

R

R

D

D

Q

A

G

G

Y

Y

E

L

P

P

I

E

A

A

I

V

T

L

S

N

H

Y

L

L

G

A

R

R

I

L

G

G

T

W

S

S

D

H

P

G

L

D

E

A

V

E

G

I

A

F

S

T

V

R

E

E

A

Q

L

F

G

V

Q

E

S

W

F

F

A

D

F

L

S

E

K

H

M

L

G

G

R

K

S

S

P

P

A

A

R

Q

Y

Y

D

D

T

H

A

N

D

K

L

L

D

N

R

W

L

L

N

N

A

N

Q

H

A

Y

L

I

H

K

V

E

M

A

D

D

-

D

-

A

-

R

-

L

Y

A

E

G

T

L

A

A

Q

K

P

P

R

F

L

F

Q

A

A

A

L

L

G

G

A

I

D

D

L

A

G

G

active site: S10 (= S10), T214 (≠ L232), K225 (= K243)
binding glutamic acid: R6 (= R6), A8 (= A8), S10 (= S10), E42 (≠ D42), R184 (= R202), H188 (= H206)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
31% identity, 98% coverage: 3:439/444 of query aligns to 103:555/564 of 3al0C

query
sites
3al0C

V

V

K

R

V

V

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

K

H

L

L

H

H

V

V

G
|
G

N

G

V

A

R

R

T
|
T

A

A

L

L

V

F

N

N

W

W

M

M

F

F

A

A

K

R

G

K

Q

E

G

G

S

K

F

F

V

I

L

L

R

R

I

I

D
x
E

D

D

T
|
T

D
|
D

L

T

A

E

R
|
R

S

S

T

S

P

R

E

E

F

Y

E

E

Q

Q

G

Q

I

I

E

L

D

E

D

S

L

L

T

R

W

W

L

C

G

G

M

L

P

D

W

W

D

D

E

E

-

G

-

P

-

D

-

I

-

G

-

D

-

F

-

G

R

P

Y

Y

N

R

Q

Q

S

S

K

E

R

R

F

L

D

E

R

I

Y

Y

E

R

E

E

A

Y

A

A

A

E

S

K

L

L

K

V

A

E

S

D

G

K

R

R

L

A

Y

Y

P

-

A

-

Y

Y

E

V

T

V

A
x
Y

D

D

E

K

L

E

D

D

R
x
P

R

S

R

K

K

E

V

-

Q

-

L

-

S

-

R

-

G

-

L

-

P

-

I

-

Y

-

D

-

R

-

A

-

L

L

E

F

L

T

T

T

E

Y

E

E

Q

Y

K

P

A

H

A

E

Y

Y

E

K

A

E

E

K

G

G

R

H

R

P

P

V

H

T

W

I

R

K

F

F

K

K

L

V

D

L

G

P

K

G

R

K

V

T

A

S

W

F

E

E

D

D

L

L

A

L

R
x
K

G

G

H

Y

A
x
M

E

E

V

F

D

D

T

N

A

S

S

T

M

L

S

E

D

D

P

F

V
x
I

L

I

I

M

R
x
K

E
x
S

D

N

G

G

L

F

F

P

L

T

Y
|
Y

T

N

L

F

P

A

S

V

V

V

I

V

D

D

I

H

D

L

M

M

G

R

I

I

T

S

H

H

I

V

I

F

R
|
R

G
|
G

E

E

D
|
D

H
|
H

V
x
L

T
x
S

N
|
N

T

T

G

P

A
x
K

Q

Q

I

L

E

M

I

I

F

Y

E

E

A

A

L

F

G

G

A

W

T

E

V

A

P

P

G

V

F

F

A

M

H

H

M

I

P

P

L
|
L

L
x
I

V

L

G

G

A

S

D

D

G
x
R

A

T

A
x
P

L
|
L

S

S

K
|
K

R

R

L

H

G

G

S

A

L

T

S

S

I

V

S

E

D

H

M

F

R

R

D

R

Q

E

G

G

Y

I

E

L

P

S

I

R

A

A

I

L

T

M

S

N

H

Y

L

L

G

A

R

L

I

L

G
|
G

T
x
W

S
x
R

D

V

P

E

L

G

E

D

V
x
E

G

I

A

F

S

T

V

I

E

E

A

E

L

K

G

L

Q

Q

S

S

F

F

A

D

F

P

S

K

K

D

M

I

G
x
S

R

N

S
x
K

P

G

A
x
V

R
x
I

Y

F

D

D

T

Y

A

Q

D
x
K

L

L

D

E

R
x
W

L

V

N

N

A

G

Q

K

A

H

L

M

H
x
R

V

R

M

I

D

D

Y

L

E

E

T

D

A

L

Q

K

P

R

R

E

L

F

Q

I

A

E

L

W

G

A

A

K

D

Y

L

A

G

G

E

K

A

E

-

I

-

P

-

S

-

V

-

D

-

E

-

R

-

Y

F

F

W

S

N

E

T

T

V

L

R

R

G

I

N

C

L

R

T
x
E

K
|
K

F

V

A

N

D

T

V

L

A

S

D
x
Q

M

L

A

Y

Q

D

I
|
I

V

M

R

Y

G

P

P

F

I

M

-

N

-

D

-

Y

-

E

-

Y

-

E

Q

K

P

D

V

Y

I

V

E

E

D

K

A

F

T

L

F

K

I

R

E

E

T

E

A

A

L

E

R

R

L

V

L

L

P

E

E

E

-

A

-

K

-

A

V

F

I

K

D

D

E

L

N

N

A

S

W

W

S

N

-

M

-

E

-

I

A

E

W

K

T

T

S

L

A
x
R

V

D

K

L

A

S

E

E

T

K

G

G

-

L

A

A

K

S

G

K

K
|
K

A

V

L

V

F

F

M
x
Q

P

L

L

I

R
|
R

L

G

A

A

L

V

T

T

G

G

Q

K

Q

L

H
x
V

G
x
T

P

P

D
x
G

M
x
L

A

F

A

E

M

T

A

I

P

E

L

V

I

L

G

G

R

K

E

E

T

R

I

T

Q

L

R

K

R

R

L

L

active site: S110 (= S10), K335 (= K243)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R6), A108 (= A8), P109 (= P9), G118 (= G18), T122 (= T22), E142 (≠ D42), Y276 (= Y184), R294 (= R202), G295 (= G203), D297 (= D205), H298 (= H206), L324 (= L232), I325 (≠ L233), L333 (= L241)
binding : T144 (= T44), D145 (= D45), R148 (= R48), Y208 (≠ A101), P213 (≠ R106), K252 (≠ R160), M255 (≠ A163), I266 (≠ V174), K269 (≠ R177), S270 (≠ E178), Y276 (= Y184), D297 (= D205), H298 (= H206), L299 (≠ V207), S300 (≠ T208), N301 (= N209), K304 (≠ A212), R330 (≠ G238), P332 (≠ A240), G363 (= G271), W364 (≠ T272), R365 (≠ S273), E370 (≠ V278), S387 (≠ G295), K389 (≠ S297), V391 (≠ A299), I392 (≠ R300), K397 (≠ D305), W400 (≠ R308), R407 (≠ H315), E446 (≠ T346), K447 (= K347), Q453 (≠ D353), I457 (= I357), R509 (≠ A394), K520 (= K404), Q524 (≠ M408), R527 (= R411), V535 (≠ H419), T536 (≠ G420), G538 (≠ D422), L539 (≠ M423)

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
30% identity, 99% coverage: 2:440/444 of query aligns to 1:476/485 of 4griB

query
sites
4griB

S

S

V

T

K

R

V

V

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

T

T

G

G

K

L

L

Q

H

H

V

I

G

G

N

G

V

I

R

R

T

T

A

A

L

L

V

F

N

N

W

Y

M

F

F

F

A

A

K

K

G

S

Q

C

G

G

S

K

F

F

V

L

L

L

R

R

I

I

D
x
E

D

D

T

T

D

D

L

Q

A

S

R

R

S

Y

T

S

P

P

E

E

F

A

E

E

Q

N

G

D

I

L

E

Y

D

S

L

L

T

K

W

W

L

L

G

G

M

I

P

S

W

F

D

D

E

E

-

G

-

P

-

V

-

G

-

D

-

Y

-

A

R

P

Y

Y

N

V

Q

Q

S

S

K

Q

R

R

F

S

D

A

R

I

Y

Y

E

K

E

Q

A

Y

A

A

A

K

S

Y

L

L

K

I

A

E

S

S

G

G

R

H

L

A

Y

Y

P

Y

A
x
C

Y

Y

E
x
C

T

S

A

P

D

E

E

R

L

L

D

E

R

R

R

I

R

K

K

K

V

I

Q

Q

L

N

S

I

R

N

G

K

L

M

P

P

I

G

Y
|
Y

D

D

R

R

A

H

A
x
C

L

R

E

N

L

L

T

S

E

N

E

E

Q

E

K

V

A

E

A

N

Y

A

E

L

A

I

E

K

G

K

R

I

R

K

P

P

H

V

W

V

R

R

F

F

K

K

-

I

-

P

L

L

D

E

G

G

K

D

R

-

V

T

A

S

W

F

E

D

D

D

L

I

A

L

R

L

G

G

H

R

A

I

E

T

V

W

D

A

T

N

A

K

S

D

M

I

S

S

-

P

D

D

P

P

V

V

L

I

I

L

R

K

E

S

D

D

G

G

L

L

F

P

L

T

Y
|
Y

T

H

L

L

P

A

S

N

V

V

I

V

D

D

I

Y

D

L

M

M

G

K

I

I

T

T

H

H

I

V

I

L

R
|
R

G

A

E

Q

D

E

H
x
W

V

V

T

S

N

S

T

G

G

P

A

L

Q

H

I

V

E

L

I

L

F

Y

E

K

A

A

L

F

G

K

A

W

T

K

V

P

P

P

G

I

F

Y

A

C

H

H

M

L

P

P

L

M

L

V

V

M

G

G

A

N

D

D

G

G

A

Q

A

K

L

L

S

S

K
|
K

R

R

L

H

G

G

S

S

L

T

S

A

I

L

S

R

D

Q

M

F

R

I

D

E

Q

D

G

G

Y

Y

E

L

P

P

I

E

A

A

I

I

T

I

S

N

H

Y

L

V

G

T

R

L

I

L

G

G

T

W

S

S

-

Y

D

D

P

D

L

K

E

R

V

E

G

F

A

F

S

S

V

K

E

N

A

D

L

L

G

E

Q

Q

S

F

F

F

A

S

F

I

S

E

K

K

M

I

G

N

R

K

S

S

P

P

A

A

R

I

Y

F

D

D

T

Y

A

H

D

K

L

L

D

D

R

F

L

F

N

N

A

S

Q

Y

A

Y

L

I

H

R

V

E

M

K

D

K

-

D

-

E

-

D

-

L

Y

F

E

N

T

L

A

L

Q

L

P

P

R

F

L

F

Q

Q

A

K

L

K

G

G

-

Y

-

V

-

S

-

K

-

P

A

S

D

T

L

L

G

E

E

E

A

N

-

Q

-

K

-

L

-

K

-

L

F

L

W

I

N

P

T

L

V

I

R

K

G

S

N

R

L

I

T

K

K

K

F

L

A

S

D

D

V

A

A

L

D

N

M

M

A

T

Q

K

I

F

V

F

R

Y

G

E

P

D

I

I

Q

K

P

S

V

W

I

N

E

L

D

D

-

E

-

F

-

L

-

S

-

R

-

K

-

T

A

A

T

K

F

E

I

V

E

C

T

S

A

I

L

L

R

E

L

L

L

I

P

K

E

P

V

I

I

L

D

E

-

G

-

F

E

E

N

K

A

R

W

S

S

S

A

E

W

E

T

N

S

D

A

K

V

I

K

F

A

Y

E

D

T

F

G

A

A

E

K

S

G

N

K

L

A

G

-

E

L

I

F

L

M

L

P

P

L

I

R

R

L

I

A

A

L

A

T

L

G

G

Q

S

Q

K

H

V

G

S

P

P

D

P

M

L

A

F

A

D

M

S

A

L

P

K

L

L

I

I

G

G

R

K

E

S

T

K

I

V

Q

F

R

E

R

R

L

I

R

K

active site: S9 (= S10), K253 (= K243)
binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (≠ D42), Y194 (= Y184), R212 (= R202), W216 (≠ H206)
binding zinc ion: C105 (≠ A97), C107 (≠ E99), Y128 (= Y120), C132 (≠ A124)

6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid
31% identity, 98% coverage: 2:437/444 of query aligns to 2:493/502 of 6brlA

query
sites
6brlA

S

K

V

V

K

R

V

V

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

K

P

L

L

H

H

V

L

G

G

N

G

V

V

R

R

T

T

A

A

L

L

V

Y

N

D

W

Y

M

L

F

F

A

A

K

K

G

H

Q

N

G

G

S

D

F

F

V

I

L

L

R

R

I

I

D
x
E

D

D

T

T

D

D

L

T

A

Q

R

R

S

Y

T

V

P

P

E

G

F

S

E

E

Q

E

G

Y

I

I

E

M

D

E

D

A

L

L

T

E

W

W

L

I

G

G

M

M

P

V

W

P

D

D

E

E

R

S

-

P

-

K

-

H

-

G

-

P

-

Y

-

A

-

P

Y

Y

N

R

Q

Q

S

S

K

E

R

R

F

R

D

D

R

I

Y

Y

E

D

E

R

A

Y

A

T

A

E

S

Q

L

I

K

L

A

K

S

T

G

D

R

Y

L

A

Y

Y

P

L

A

A

Y

F

E

D

T

T

A

P

D

E

E

E

L

L

D

D

R

Q

R

I

R

R

K

A

V

E

Q

F

L

E

S

A

R

R

G

G

L

D

P

V

P

F

I

A

Y

Y

D

N

-

Y

-

E

-

T

-

R

-

N

-

R

-

L

R

R

A

N

A

S

L

I

E

S

L

L

T

P

E

E

Q

E

K

V

A

K

Y

L

E

L

A

E

E

E

G

K

R

T

R

P

P

Y

H

V

W

I

R

R

F

F

K

K

L

M

D

P

G

L

K

D

R

R

V

I

A

I

-

N

W

L

E

N

D

D

L

I

A

I

R

R

G

G

H

K

A

F

E

S

V

V

D

N

T

T

A

N

S

T

M

L

S

D

D

D

P

K

V

V

L

L

I

V

R

K

E

N

D

D

G

G

L

M

F

P

L

T

Y
|
Y

T

H

L

F

P

A

S
x
N

V

I

I

I

D

D

I

H

D

E

M

M

G

K

I

I

T

T

H

H

I

V

I

I

R
|
R

G

G

E

E

D

E

H
x
W

V

L

T

P

N

S

T

M

G

A

A

L

Q

H

I

V

E

L

I

L

F

Y

E

E

A

A

L

M

G

G

A

W

T

D

V

A

P

P

G

E

F

F

A

A

H

H

M

L

P

S

L

L

L

I

V

L

G

K

A

P

D

E

G

G

-

K

A

G

A

K

L

L

S

S

K
|
K

R

R

L

D

G

G

S

D

-

K

-

F

-

G

-

F

-

P

-

V

-

F

-

P

L

L

S

N

I

F

S

T

D

D

-

S

-

A

-

T

-

G

-

N

-

T

-

S

-

A

-

G

M

Y

R

R

D

E

Q

E

G

G

Y

Y

E

L

P

P

I

E

A

A

I

F

T

I

S

N

H

M

L

V

G

A

R

M

I

L

G

G

T

W

S

S

-

P

-

A

D

D

P

N

L

K

E

E

V

I

G

-

A

V

S

S

V

M

E

D

A

E

L

M

G

I

Q

K

S

E

F

F

A

D

F

L

S

N

K

K

M

V

G

H

R

K

S

A

P

G

A

A

R

R

Y

F

D

S

T

A

A

E

D

K

L

A

D

K

R

W

L

F

N

N

A

Q

Q

Q

A

Y

L

L

H

Q

V

L

M

M

D

S

Y

N

E

E

T

A

A

I

Q

L

P

P

R

E

L

F

Q

K

A

K

L

V

G

L

A

A

D

E

L

-

G

-

E

-

A

-

F

-

W

-

N

N

T

N

V

V

R

E

G

V

N

S

L

D

T

E

K

K

F

A

A

L

D

K

V

I

A

I

D

G

M

L

A

M

Q

K

I

E

V

R

R

A

G

T

P

F

I

V

Q

K

P

D

V

I

I

Y

E

N

D

D

A

G

T

K

F

F

I

F

E

F

T

H

A

A

L

P

R

E

L

S

L

F

P

D

E

E

V

K

I

A

D

S

E

K

N

K

A

A

W

W

S

S

A

P

W

E

T

T

S

A

A

V

V

L

-

M

-

Q

-

E

-

L

-

T

-

E

-

A

-

I

-

S

-

L

-

D

-

F

K

K

A

A

E

E

T

I

-

I

-

K

-

E

-

S

-

I

-

H

-

L

-

A

G

E

A

A

K

K

G

G

K

L

A

G

L

M

-

G

-

K

-

V

F

M

M

M

P

P

L

L

R

R

L

L

A

S

L

L

T

V

G

G

Q

E

Q

L

H

K

G

G

P

P

D

D

M

V

A

P

A

D

M

L

A

M

P

E

L

M

I

I

G

G

R

K

-

E

E

E

T

T

I

I

Q

S

R

R

active site: K261 (= K243)
binding glutamic acid: R6 (= R6), A8 (= A8), S10 (= S10), E42 (≠ D42), Y201 (= Y184), N205 (≠ S188), R219 (= R202), W223 (≠ H206)

8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
34% identity, 80% coverage: 2:355/444 of query aligns to 3:364/488 of 8vc5A

query
sites
8vc5A

S

T

V

V

K

R

V

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

K

D

L

P

H

H

V

V

G

G

N

T

V

A

R

Y

T

I

A

A

L

L

V

F

N

N

W

L

M

C

F

F

A

A

K

R

G

Q

Q

H

G

G

S

Q

F

F

V

I

L

L

R

R

I

I

D

E

D

D

T

T

D

D

L

Q

A

L

R

R

S

S

T

T

P

R

E

E

F

S

E

E

Q

Q

G

Q

I

I

E

Y

D

D

D

A

L

L

T

R

W

W

L

L

G

G

M

I

P

E

W

W

D

D

E

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

-

G

R

P

Y

Y

N

R

Q

Q

S

S

K

E

R

R

F

G

D

H

R

I

Y

Y

E

K

A

Y

A

S

A

D

S

E

L

L

K

V

A

E

S

K

G

G

R

H

L

A

Y

F

P

T

A
x
C

Y

F

E
x
C

T

T

A

P

D

E

E

R

L

L

D

D

R

A

R

V

R

R

K

A

V

E

Q

Q

L

M

S

A

R

R

G

K

L

E

P

T

P

P

I

R

Y
|
Y

D

D

R

G

A

H

A
x
C

L

M

E

H

L

L

T

P

E

K

E

D

Q

E

K

V

A

Q

A

R

Y

R

E

L

A

A

E

A

G

G

R

E

R

S

P

H

H

V

W

T

R

R

F

M

K

K

L

V

D

P

G

T

K

E

R

G

V

V

A

C

W

V

-

V

E

P

D

D

L

M

A

L

R

R

G

G

H

D

A

V

E

E

V

I

D

P

T

W

A

D

S

R

M

M

S

D

D

M

P

Q

V

V

L

L

I

M

R

K

E

A

D

D

G

G

L

L

F

P

L

T

Y

Y

T

F

L

L

P

A

S

N

V

V

I

V

D

D

I

H

D

L

M

M

G

G

I

I

T

T

H

H

I

V

I

L

R

R

G

G

E

E

D

E

H

W

V

L

T

P

N

S

T

A

G

P

A

K

Q

L

I

I

E

K

I

L

F

Y

E

E

A

Y

L

F

G

G

A

W

T

E

V

Q

P

P

G

Q

F

L

A

C

H

Y

M

M

P

P

L

L

V

R

G

N

A

P

D

D

G

K

A

S

A

K

L

L

S

S

K

K

R

R

L

K

G

N

S

P

L

T

S

S

I

I

S

T

D

F

M

Y

R

E

D

R

Q

M

G

G

Y

Y

E

L

P

P

I

Q

A

A

I

L

T

L

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N

H

Y

L

L

G

G

R

R

I

M

G

G

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W

S

S

D

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P

K

L

F

E

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V

L

G

A

A

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S

M

V

I

E

E

A

-

L

-

G

-

Q

-

S

H

F

F

A

D

F

L

S

S

K

R

M

V

G

S

R

L

S

G

P

G

A

P

R

I

Y

F

D

D

T

L

A

E

D

K

L

L

D

S

R

W

L

L

N

N

A

G

Q

Q

A

W

L

I

H

R

V

E

M

Q

D

S

Y

V

E

E

T

E

A

F

Q

A

P

R

R

E

L

V

Q

Q

-

K

-

W

A

A

L

L

G

N

A

P

D

E

L

Y

G

L

E

M

A

K

F

I

W

A

N

P

T

H

V

V

R

Q

G

G

N

R

L

V

T

E

K

N

F

F

A

S

D

Q

V

I

A

A

D

P

M

L

A

A

binding zinc ion: C107 (≠ A97), C109 (≠ E99), Y130 (= Y120), C134 (≠ A124)

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
31% identity, 98% coverage: 3:439/444 of query aligns to 2:463/468 of 1g59A

query
sites
1g59A

V

V

K

V

V

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

K

D

L

P

H

H

V

V

G

G

N

T

V

A

R

Y

T

I

A

A

L

L

V

F

N

N

W

Y

M

A

F

W

A

A

K

R

G

R

Q

N

G

G

S

R

F

F

V

I

L

V

R

R

I

I

D

E

D

D

T

T

D
|
D

L
x
R

A
|
A

R
|
R

S

Y

T

V

P

P

E

G

F

A

E

E

Q

E

G

R

I

I

E

L

D

A

L

L

T

K

W

W

L

L

G

G

M

L

P

S

W

Y

D

D

E

E

-

G

-

P

-

D

-

V

-

A

-

P

-

T

-

G

R

P

Y

Y

N

R

Q

Q

S

S

K

E

R

R

F

L

D

P

R

L

Y

Y

E

Q

E

K

A

Y

A

A

A

E

S

E

L

L

K

L

A

K

S

R

G

G

R

W

L

A

Y

Y

P

R

A

A

Y

F

E

E

T

T

A
x
P

D

E

E

E

L

L

D

E

R

Q

R

I

R

R

K

K

V

E

Q

K

-

G

-

Y

-

D

-

G

L

R

S

A

R

R

G

N

L

I

P

P

I

-

Y

-

D

-

R

-

A

-

L

-

E

-

L

-

T

-

E

-

Q

E

K

E

A

A

A

E

Y

E

E

R

A

A

E

R

G

R

R

G

R

E

P

P

H

H

W

-

R
x
V

F

I

K

R

L

L

D

K

G

V

K

P

R

R

-

P

-

G

-

T

V

T

A

E

W

V

E

K

D

D

L

E

A

L

R
|
R

G

G

H

V

A
x
V

E

V

V

Y

D

D

T

N

A

Q

S
x
E

M

I

S

P

D

D

P

V

V
|
V

L

L

I

L

R
x
K

E
x
S

D
|
D

G

G

L

Y

F

P

L

T

Y

Y

T

H

L

L

P

A

S

N

V

V

I

V

D

D

I

H

D

L

M

M

G

G

I

V

T

T

H

D

I

V

I

I

R

R

G

A

E
|
E

D
x
E

H

W

V

L

T

V

N

S

T

T

G

P

A

I

Q

H

I

V

E

L

I

L

F

Y

E

R

A

A

L

F

G

G

A

W

T

E

V

A

P

P

G

R

F

F

A

Y

H

H

M

M

P

P

L

L

V
x
R

G

N

A

P

D

D

G
x
K

A
x
T

A
x
K

L

I

S

S

K

K

R

R

L

K

G

S

S

H

L

T

S

S

I

L

S

D

D

W

M

Y

R

K

D

A

Q

E

G

G

Y

F

E

L

P

P

I

E

A

A

I

L

T

R

S

N

H

Y

L

L

G

C

R

L

I
x
M

G
|
G

T

F

S

S

D

M

P

P

-

D

-

G

L

R

E
|
E

V

I

G

-

A

F

S

T

V

L

E

E

A

E

L

F

G

I

Q

Q

S

A

F

F

A

T

F

W

S

E

K

R

M

V

G
x
S

R
x
L

S

G

P

G

A
x
P

R
x
V

Y

F

D

D

T

L

A

E

D
x
K

L

L

D

R

R
x
W

L

M

N

N

A

G

Q

K

A

Y

L

I

-
x
R

H

E

V

V

M

L

D

S

Y

L

E

E

T

E

A

V

Q

A

P

E

R

R

L

V

Q

K

A

P

L

F

G

L

A

R

D

E

L

A

G

G

-

L

-

S

-

W

-

E

-

S

E

E

A

A

F

Y

W

L

-

R

-

A

-

V

N

E

T

L

V

M

R

R

G
x
P

N
x
R

L

F

T

D

K

T

F

L

A

K

D

E

V

F

A

P

D

E

M

K

A

A

Q

R

I

Y

V

L

R

F

G

T

P

E

I

D

Q

Y

P

P

V

V

I

S

E

E

D

K

A

A

T

Q

F

R

-

K

I

L

E

E

T

E

A

G

L

L

R

P

L

L

P

K

E

E

V

L

I

Y

D

P

E

R

-

L

N

R

A

A

W

Q

S

E

A

E

W

W

T

T

S

E

A

A

-

A

-

L

-

E

-

A

-

L

-
x
R

-

G

V

F

K

A

A

A

E

E

T

K

G

G

A

V

K
|
K

G
x
L

K

G

A

Q

L

V

F

A

M
x
Q

P

P

L

L

R
|
R

L

A

A

A

L

L

T

T

G

G

Q

S

Q
x
L

H
x
E

G
x
T

P
|
P

D
x
G

M
x
L

A
x
F

A

E

M

I

A

L

P

A

L

L

I

L

G

G

R

K

E

E

T

R

I

A

Q

L

R

R

L

L

binding : D44 (= D45), R45 (≠ L46), A46 (= A47), R47 (= R48), P109 (≠ A101), V145 (≠ R145), R163 (= R160), V166 (≠ A163), E172 (≠ S169), V177 (= V174), K180 (≠ R177), S181 (≠ E178), D182 (= D179), E207 (= E204), E208 (≠ D205), R237 (≠ V234), K241 (≠ G238), T242 (≠ A239), K243 (≠ A240), M273 (≠ I270), G274 (= G271), E282 (= E277), S299 (≠ G295), L300 (≠ R296), P303 (≠ A299), V304 (≠ R300), K309 (≠ D305), W312 (≠ R308), R319 (vs. gap), P357 (≠ G343), R358 (≠ N344), R417 (vs. gap), K426 (= K402), L427 (≠ G403), Q432 (≠ M408), R435 (= R411), L442 (≠ Q418), E443 (≠ H419), T444 (≠ G420), P445 (= P421), G446 (≠ D422), L447 (≠ M423), F448 (≠ A424)

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
31% identity, 98% coverage: 3:439/444 of query aligns to 2:463/468 of 2cv2A

query
sites
2cv2A

V

V

K

V

V

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

K

D

L

P

H

H

V

V

G
|
G

N

T

V

A

R

Y

T
x
I

A

A

L

L

V

F

N

N

W

Y

M

A

F

W

A

A

K

R

G

R

Q

N

G

G

S

R

F

F

V

I

L

V

R

R

I

I

D
x
E

D

D

T
|
T

D
|
D

L

R

A

A

R
|
R

S

Y

T

V

P

P

E

G