SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066553020.1 NCBI__GCF_001636925.1:WP_066553020.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
45% identity, 99% coverage: 3:244/244 of query aligns to 64:310/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 99% coverage: 3:244/244 of query aligns to 4:248/249 of P12282

query
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P12282

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R14 (= R13) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S43) mutation to A: No effect.
R73 (= R72) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G127) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D129) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C141) mutation to A: No effect.
C172 (= C168) mutation to A: No zinc bound and no enzyme activity.
C175 (= C171) mutation to A: No zinc bound and no enzyme activity.
C187 (= C183) mutation to A: No effect.
C231 (≠ G227) mutation to A: No effect.
C244 (= C240) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C243) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
44% identity, 99% coverage: 3:244/244 of query aligns to 3:240/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R13), D129 (= D129)
binding adenosine monophosphate: G37 (= G37), G39 (= G39), G40 (= G40), D61 (= D61), F62 (≠ D62), K85 (= K85), L108 (≠ R108), C127 (≠ G127), T128 (= T128), D129 (= D129), N130 (≠ D130), V133 (≠ T133)
binding zinc ion: C171 (= C168), C236 (= C240), C239 (= C243)

1jw9B Structure of the native moeb-moad protein complex (see paper)
44% identity, 99% coverage: 3:244/244 of query aligns to 3:240/240 of 1jw9B

query
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1jw9B

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active site: R13 (= R13), D129 (= D129)
binding zinc ion: C171 (= C168), C236 (= C240), C239 (= C243)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
45% identity, 100% coverage: 1:244/244 of query aligns to 1:253/271 of Q72J02

query
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Q72J02

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L

L

T

P

G

A

V

V

I

V

G

G

S

S

M

L

A

M

A

A

L

A

E

E

V

A

V

L

K

K

L

V

I

L

T

L

G

G

A

I

G

G

E

K

A

P

L

L

T

A

G

G

R

H

L

L

M

L

L

L

Y

Y

D

D

G

A

L

L

A

E

G

A

S

S

A

F

R

R

T

K

V

L

R

T

V

V

A

R

D

N

P

P

E

R

C

C

P

P

V

V

C

C

G

G

C192 (= C183) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
44% identity, 100% coverage: 1:244/244 of query aligns to 1:249/270 of D4GSF3

query
sites
D4GSF3

M

M

I

T

F

L

S

S

E

L

D

D

-

A

-

T

E

Q

V

L

E

D

R

R

Y

Y

A

S

R

R

H

H

L

I

V

I

L

M

S

D

E

E

V

V

G

G

G

P

P

E

G

G

Q

Q

Q

G

A

R

L

L

K

L

R

S

A

S

R

R

V

V

L

V

I

V

V

V

G

G

A

A

G

G

V

L

G

G

S

A

P

P

A

A

A

I

L

Q

Y

Y

L

L

A

A

A

V

G

G

V

V

G

G

A

E

L

L

G

V

L

V

I

V

D

D

V

V

G

E

L

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

V

A

V

F

H

S

C

T

D

A

D

Q

D

V

V

G

G

R

T

S

P

K

K

V

A

E

E

A

S

A

A

E

A

R

F

L

V

V

R

G

G

L

L

N

N

P

P

H

D

V

V

A

S

I

V

E

E

T

P

F

V

A

E

E

A

R

R

I

V

T

D

A

K

E

S

N

N

A

V

A

H

A

E

R

V

I

V

E

A

A

G

F

S

D

D

I

V

V

V

L

V

D

D

G

A

T

S

D

D

D

N

F

F

Q

P

T

T

R

R

L

Y

W

L

V

L

N

N

A

D

A

V

C

C

V

R

A

F

A

E

G

G

K

I

P

P

L

L

V

V

S

H

G

G

A

A

L

I

G

Y

R

K

W

F

N

E

G

G

Q

Q

V

A

G

T

V

T

F

L

S

V

G

P

Q

D

-

G

P

P

C

C

Y

Y

Q

R

C

C

L

L

V

F

P

P

E

E

I

A

P

P

-

E

-

P

-

G

-

T

-

V

P

P

D

D

A

-

E

-

T

-

C
|
C

A

A

R

T

V

T

G

G

V

V

V

L

G

G

A

V

L

L

T

P

G

G

V

T

I

V

G

G

S

C

M

I

A

Q

A

A

L

T

E

E

V

A

V

M

K

K

L

L

I

L

T

L

G

D

A

E

G

G

E

E

A

A

L

L

T

D

G

G

R

R

L

L

M

L

L

F

Y

Y

D

D

G

A

L

M

A

D

G

M

S

T

A

F

R

E

T

T

V

V

R

P

V

Y

A

R

A

T

D

N

P

P

E

D

C

C

P

P

V

V

C

C

G

G

C188 (= C183) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 99% coverage: 3:244/244 of query aligns to 1:244/244 of 1zfnA

query
sites
1zfnA

F

M

S

N

E

D

D

R

E

D

V

F

E

M

R

R

Y

Y

A

S

R
|
R

H

Q

L

I

V

L

L

L

S

D

E

D

V

I

G

A

G

L

P

D

G

G

Q

Q

A

K

L

L

K

L

R

D

A

S

R

Q

V

V

L

L

I

I

V
x
I

G
|
G

A

L

G
|
G

V

L

G

G

S

T

P

P

A

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

L

G

V

L

L

I

A

D
|
D

D

D

V

D

V

V

G

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

I

I

A

L

F

F

S

T

T

T

A

E

Q

D

V

I

G

D

R

R

S

P

K
|
K

V

S

E

Q

A

V

A

S

A

Q

E

Q

R

R

L

L

V

T

G

Q

L

L

N

N

P

P

H

D

V

I

A

Q

I

L

E

T

T

A

F

L

A

Q

E

Q

R

R

I

L

T

T

A

G

E

E

N

A

A

L

A

K

A

D

R

A

I

V

E

A

A

R

F

A

D

D

I

V

V

V

L

L

D

D

G

C

T
|
T

D
|
D

D

N

F

M

Q

A

T
|
T

R

R

L

Q

W

E

V

I

N

N

A

A

C

C

V

V

A

A

A

L

G

N

K

T

P

P

L

L

V

I

S

T

G

A

A

S

L

A

G

V

R

G

W

F

N

G

G

G

Q

Q

V

L

G

M

V

V

F

L

S

T

-

P

-

W

G

E

Q

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

L

L

V

W

P

P

E

D

I

N

P

Q

P

E

D

P

A

E

E

R

T

N

C

C

A

R

R

T

V

A

G

G

V

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

S

T

M

L

A

Q

A

A

L

L

E

E

V

A

V

I

K

K

L

L

I

L

T

S

G

G

A

I

G

-

E

E

A

T

L

P

T

A

G

G

R

E

L

L

M

R

L

L

Y

F

D

D

G

G

L

K

A

S

G

S

S

Q

A

W

R

R

T

S

V

L

R

A

V

L

A

R

D

A

P

S

E

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R13), D127 (= D129)
binding adenosine-5'-triphosphate: I34 (≠ V36), G35 (= G37), G37 (= G39), G38 (= G40), D59 (= D61), R70 (= R72), Q71 (= Q73), K83 (= K85), T126 (= T128), D127 (= D129), T131 (= T133)
binding zinc ion: C169 (= C168), C172 (= C171), C240 (= C240), C243 (= C243)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 99% coverage: 3:244/244 of query aligns to 1:244/251 of P30138

query
sites
P30138

F

M

S

N

E

D

D

R

E

D

V

F

E

M

R

R

Y

Y

A

S

R

R

H

Q

L

I

V

L

L

L

S

D

E

D

V

I

G

A

G

L

P

D

G

G

Q

Q

A

K

L

L

K

L

R

D

A

S

R

Q

V

V

L

L

I

I

V

I

G

G

A

L

G

G

V

L

G

G

S

T

P

P

A

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

L

G

V

L

L

I

A

D

D

V

D

V

V

G

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

A

L

F

F

S

T

T

T

A

E

Q

D

V

I

G

D

R

R

S

P

K

K

V

S

E

Q

A

V

A

S

A

Q

E

Q

R

R

L

L

V

T

G

Q

L

L

N

N

P

P

H

D

V

I

A

Q

I

L

E

T

T

A

F

L

A

Q

E

Q

R

R

I

L

T

T

A

G

E

E

N

A

A

L

A

K

A

D

R

A

I

V

E

A

A

R

F

A

D

D

I

V

V

V

L

L

D

D

G

C

T

T

D

D

D

N

F

M

Q

A

T

T

R

R

L

Q

W

E

V

I

N

N

A

A

C

C

V

V

A

A

A

L

G

N

K

T

P

P

L

L

V

I

S

T

G

A

A

S

L

A

G

V

R

G

W

F

N

G

G

G

Q

Q

V

L

G

M

V

V

F

L

S

T

-

P

-

W

G

E

Q

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

L

L

V
x
W

P

P

E

D

I

N

P

Q

P

E

D

P

A

E

E

R

T

N

C
|
C

A

R

R

T

V

A

G

G

V

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

S

T

M

L

A

Q

A

A

L

L

E

E

V

A

V

I

K

K

L

L

I

L

T

S

G

G

A

I

G

-

E

E

A

T

L

P

T

A

G

G

R

E

L

L

M

R

L

L

Y

F

D

D

G

G

L

K

A

S

G

S

S

Q

A

W

R

R

T

S

V

L

R

A

V

L

A

R

D

A

P

S

E

G

C
|
C

P

P

V

V

C
|
C

G

G

C169 (= C168) binding Zn(2+)
C172 (= C171) binding Zn(2+)
W174 (≠ V173) mutation to A: No adenylation of ThiS.
C184 (= C183) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C240) binding Zn(2+)
C243 (= C243) binding Zn(2+)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
48% identity, 92% coverage: 3:227/244 of query aligns to 55:283/460 of O95396

query
sites
O95396

F

L

S

S

E

R

D

D

E

E

V

I

E

L

R

R

Y

Y

A

S

R

R

H

Q

L

L

V

V

L

L

S

P

E

E

V

L

G

G

G

V

P

H

G

G

Q

Q

Q

L

A

R

L

L

K

G

R

T

A

A

R

C

V

V

L

L

I

I

V

V

G

G

A

C

G

G

V

L

G

G

S

C

P

P

A

L

A

A

L

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

A

R

L

L

G

G

L

L

I

V

D

D

D

Y

D

D

V

V

G

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

I

V

A

L

F

H

S

G

T

E

A

A

Q

L

V

A

G

G

R

Q

S

A

K

K

V

A

E

F

A

S

A

A

E

A

R

S

L

L

V

R

G

R

L

L

N

N

P

S

H

A

V

V

A

E

I

C

E

V

T

P

F

Y

A
x
T

E
x
Q

R
x
A

I
x
L

T
|
T

A
x
P

E
x
A

N
x
T

A
|
A

A
x
L

A
x
D

R
x
L

I
x
V

E
x
R

A
x
R

F
x
Y

D
|
D

I
x
V

V
|
V

L
x
A

D
|
D

G
x
C

T
x
S

D
|
D

D
x
N

F
x
V

Q
x
P

T
|
T

R
|
R

L
x
Y

W
x
L

V
|
V

N
|
N

A
x
D

A
|
A

C
|
C

V
|
V

A
x
L

A
|
A

G
|
G

K
x
R

P
|
P

L
|
L

V
|
V

S
|
S

G
x
A

A
x
S

L
x
A

G
x
L

R
|
R

W
x
F

N
x
E

G
|
G

Q
|
Q

V
x
I

G
x
T

V
|
V

-
x
Y

-
x
H

F
x
Y

S
x
D

G
|
G

Q
x
G

P
|
P

C
|
C

Y
|
Y

Q
x
R

C
|
C

L
x
I

V
x
F

P
|
P

E
x
Q

I

-

P
|
P

D
x
P

A
|
A

E
|
E

T
|
T

-
x
V

-
x
T

-
x
N

C
|
C

A

A

R

D

V

G

G

G

V

V

V

L

G

G

A

V

L

V

T

T

G

G

V

V

I

L

G

G

S

C

M

L

A

Q

A

A

L

L

E

E

V

V

V

L

K

K

L

I

I

A

T

A

G

G

A

L

G

G

E

P

A

S

L

Y

T

S

G

G

R

S

L

L

M

L

L

L

Y

F

D

D

G

A

L

L

A

R

G

G

158:238 (vs. 106:182, 41% identical) Interaction with NFS1
C239 (= C183) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
38% identity, 98% coverage: 6:244/244 of query aligns to 64:319/482 of O59954

query
sites
O59954

D

E

E

E

V

Y

E

R

R

R

Y

Y

A

G

R

R

H

Q

L

M

V

I

L

V

S

P

E

Q

V

F

G

G

G

L

P

Q

G
|
G

Q

Q

Q

L

A

K

L

L

K

R

R

D

A

A

R

K

V

V

L

L

I

I

V

V

G

G

A

A

G

G

V

L

G
|
G

S

C

P

P

A

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

I

G

G

L

L

I

V

D

D

D

G

D

D

V

T

V

V

G

E

L

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

I

V

A

L

F

H

S

R

T

S

A

R

Q

N

V

V

G

G

R

K

S

L

K

K

V

V

E

D

A

S

A

A

A

I

E

E

R

Y

L

L

V

R

G

E

L

L

N

N

P

P

H

H

V

P

A

T

I

Y

E

I

T

A

F

H

A

Q

E

A

R

H

I

L

T

T

A

P

E

R

N

E

A

A

A

P

A

D

R

I

I

F

E

K

A

D

F

Y

D

D

I

L

V

I

L

L

D

D

G
x
C

T

T

D

D

D

N

F

P

Q

A

T

T

R

R

L

Y

W

L

V

I

N

S

A

D

A

T

C

A

V

V

A

L

G

G

K

K

P

P

L

L

V

V

S

S

G

A

A

S

L

A

G

L

R

R

W

T

N
x
E

G

G

Q

Q

V

L

G

M

V

V

F

L

-

N

-

P

-

Q

-

P

-

G

-

D

-

K

S

T

G

G

Q

G

P

P

C

C

Y

Y

Q

R

C

C

L

V

V

F

P

P

E

K

I

P

P

P

-

A

-

N

D

S

A

V

E

T

T

S

C

C

A

A

R

D

V

G

G

G

V

I

V

L

G

G

A

P

L

V

T

V

G
|
G

V

T

I

M

G

G

S

V

M

L

A

Q

A

A

L

S

E

E

V

A

V

I

K

K

L

V

I

L

T

T

G

S

A

A

G

G

E

E

A

S

L

V

T

E

G

A

-

T

-

P

R

S

L

L

M

L

L

I

Y

F

D

S

G

A

L

Y

A

S

G

S

S

P

A

Q

-

F

R

R

T

T

V

I

R

K

V

L

-

R

A

S

A

R

D

R

P

P

E

N

C

C

P

A

V

V

C

C

G

S

G82 (= G24) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G42) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R72) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G127) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ N157) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G194) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

1zud3 Structure of this-thif protein complex (see paper)
40% identity, 99% coverage: 3:244/244 of query aligns to 1:239/240 of 1zud3

query
sites
1zud3

F

M

S

N

E

D

D

R

E

D

V

F

E

M

R

R

Y

Y

A

S

R
|
R

H

Q

L

I

V
x
L

L

L

S
x
D

E

D

V

I

G

A

G

L

P

D

G

G

Q

Q

A

K

L

L

K

L

R

D

A

S

R

Q

V

V

L

L

I

I

V

I

G

G

A

L

G

G

V

L

G

G

S

T

P

P

A

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

L

G

V

L

L

I

A

D

D

V

D

V

V

G

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

A

L

F

F

S

T

T

T

A

E

Q

D

V

I

G

D

R

R

S

P

K

K

V

S

E

Q

A

V

A

S

A

Q

E

Q

R

R

L

L

V

T

G

Q

L

L

N

N

P

P

H

D

V

I

A

Q

I

L

E

T

T

A

F

L

A

Q

E

Q

R

R

I

L

T

T

A

G

E

E

N

A

A

L

A

K

A

D

R

A

I

V

E

A

A

R

F

A

D

D

I

V

V

V

L

L

D

D

G

C

T

T

D
|
D

D

N

F

M

Q

A

T

T

R

R

L

Q

W

E

V

I

N

N

A

A

C

C

V

V

A

A

A

L

G

N

K

T

P

P

L

L

V

I

S

T

G

A

A

S

L

A

G

V

R

G

W

F

N

G

G

G

Q

Q

V

L

G

M

V

V

F

L

S

T

-

P

-

W

G

E

Q

Q

P

G

C
|
C

Y

Y

Q

R

C

C

L

L

V

W

P

P

E

D

I

-

P

-

D

N

A

Q

E

E

T

P

C

T

A

A

R

G

V

V

G

V

V

G

V

P

G

V

A

V

L

G

T

V

G

M

V

G

I

-

G

-

S

T

M

L

A

Q

A

A

L

L

E

E

V

A

V

I

K

K

L

L

I

L

T

S

G

G

A

I

G

-

E

E

A

T

L

P

T

A

G

G

R

E

L

L

M

R

L

L

Y

F

D

D

G

G

L

K

A

S

G

S

S

Q

A

W

R

R

T

S

V

L

R

A

V

L

A

R

D

A

P

S

E

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R13), D127 (= D129)
binding calcium ion: L14 (≠ V16), D16 (≠ S18)
binding zinc ion: C169 (= C168), C235 (= C240), C238 (= C243)

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
39% identity, 99% coverage: 3:244/244 of query aligns to 39:290/440 of P38820

query
sites
P38820

F

L

S

S

E

L

D

E

E

E

V

Y

E

Q

R

R

Y

Y

A

G

R

R

H

Q

L

M

V

I

L

V

S

E

E

E

V

T

G

G

-

V

P

A

G

G

Q

Q

Q

V

A

K

L

L

K

K

R

N

A

T

R

K

V

V

L

L

I

V

V

V

G

G

A

A

G

G

V

L

G

G

S

C

P

P

A

A

A

L

L

P

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

Q

L

I

G

G

L

I

I

V

D

D

D

N

D

D

V

V

G

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

L

F

H

S

D

T

S

A

S

Q

R

V

V

G

G

R

M

S

L

K

K

V

C

E

E

A

S

A

A

A

R

E

Q

R

Y

L

I

V

T

G

K

L

L

N

N

P

P

H

H

V

I

A

N

I

V

E

V

T

T

F

Y

A

P

E

V

R

R

I

L

T

N

A

S

E

S

N

N

A

A

A

F

A

D

R

I

I

F

E

K

A

G

F

Y

D

N

I

Y

V

I

L

L

D

D

G

C

T

T

D

D

D

S

F

P

Q

L

T

T

R

R

L

Y

W

L

V

V

N

S

A

D

A

V

C

A

V

V

A

N

A

L

G

G

K

I

P

T

L

V

V

V

S

S

G

A

A

S

L

G

G

L

R

G

W

T

N

E

G

G

Q

Q

V

L

G

T

V

I

F

L

S

N

G

F

Q

N

-

N

-

I

-

G

P

P

C

C

Y

Y

Q

R

C

C

L

F

V

Y

P

P

E

T

I

P

P

P

D

P

-

N

-

A

A

V

E

T

T

S

C
|
C

A

Q

R

E

V

G

G

G

V

V

V

I

G

G

A

P

L

C

T

I

G

G

V

L

I

V

G

G

S

T

M

M

A

M

A

A

L

V

E

E

V

T

V

L

K

K

L

L

I

I

T

L

G

G

-

I

-

Y

A

T

G

N

E

E

A

N

L

F

T

S

G

P

R

F

L

L

M

M

L

L

Y

Y

D

S

G

G

L

F

-

P

A

Q

G

Q

S

S

A

L

R

R

T

T

V

F

R

K

V

M

A

R

A

G

D

R

P

Q

E

E

-

K

C

C

P

L

V

C

C

C

G

G

C225 (= C183) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
42% identity, 95% coverage: 3:234/244 of query aligns to 3:215/217 of 1jwaB

query
sites
1jwaB

F

L

S

S

E

D

D

Q

E

E

V

M

E

L

R

R

Y

Y

A

N

R
|
R

H

Q

L

I

V

I

L

L

S

R

E

G

V

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

A

A

L

L

K

K

R

D

A

S

R

R

V

V

L

L

I

I

V

V

G

G

A

L

G
|
G

V

L

G

G

S

C

P

A

A

A

A

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

A

N

L

L

G

T

L

L

I

L

D
|
D

D
x
F

D

D

V

T

V

V

G

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

A

L

F

H

S

S

T

D

A

A

Q

T

V

V

G

G

R

Q

S

P

K
|
K

V

V

E

E

A

S

A

A

A

R

E

D

R

A

L

L

V

T

G

R

L

I

N

N

P

P

H

H

V

I

A

A

I

I

E

T

T

P

F

V

A

N

E

A

R
x
L

I

L

T

D

A

D

E

A

N

E

A

L

A

A

R

L

I

I

E

A

A

E

F

H

D

D

I

L

V

V

L

L

D

D

G

C

T

T

D
|
D

D
x
N

F

V

Q

A

T
x
V

R

R

L

N

W

Q

V

L

N

N

A

A

A

G

C

C

V

F

A

A

G

K

K

V

P

P

L

L

V

V

S

S

G

G

A

A

L

A

G

I

R

R

W

M

N

E

G

G

Q

Q

V

I

G

T

V

V

F

F

S

T

G

Y

Q

E

P

-

C

-

Y

-

Q

-

C

-

L

-

V

-

P

-

E

-

I

-

P

-

D

-

A

-

E

-

T

-

C

-

A

-

R

-

V

A

G

G

V

V

V

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

S

S

M

L

A

Q

A

A

L

M

E

E

V

A

V

I

K

K

L

M

I

L

T

A

G

G

A

Y

G

G

E

K

A

P

L

A

T

S

G

G

R

K

L

I

M

V

L

M

Y

Y

D

D

G

A

L

M

A

T

G

C

S

Q

A

F

R

R

T

E

V

M

R

K

V

L

active site: R13 (= R13), D129 (= D129)
binding adenosine-5'-triphosphate: G39 (= G39), G40 (= G40), D61 (= D61), F62 (≠ D62), R72 (= R72), K85 (= K85), L108 (≠ R108), D129 (= D129), N130 (≠ D130), V133 (≠ T133)

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 84% coverage: 3:208/244 of query aligns to 7:212/289 of 6yubB

query
sites
6yubB

F

L

S

S

E

K

D

E

E

E

V

I

E

E

R

R

Y

Y

A

S

R

R

H

Q

L

M

V

I

L

V

S

P

E

G

V

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

A

R

L

L

K

M

R

N

A

A

R

K

V

V

L

L

I

I

V

I

G

G

A

A

G

G

V

L

G

G

S

C

P

P

A

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

I

G

G

L

I

I

V

D

D

D

G

D

D

V

S

V

V

G

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

A

F

H

S

A

T

T

A

K

Q

R

V

V

G

G

R

M

S

L

K

K

V

V

E

D

A

S

A

L

A

I

E

T

R

H

L

L

V

I

G

E

L

I

N

N

P

P

H

L

V

P

A

V

I

Y

E

V

T

P

F

Y

A

R

E

F

R

D

I

L

T

T

A

P

E

Q

N

N

A

A

A

Q

R

I

I

I

E

K

A

P

F

W

D

D

I

V

V

I

L

L

D

D

G

C

T

T

D

D

D

N

F

P

Q

A

T

T

R

R

L

Y

W

L

V

I

N

S

A

D

A

V

C

C

V

V

A

L

G

G

K

K

P

P

L

L

V

V

S

S

G

A

A

A

L

S

G

V

R

Q

W

K

N

S

G

G

Q

Q

V

L

G

I

V

V

F

L

S

N

G

C

Q

P

P

P

C

T

Y

P

Q

Q

C

G

L

V

V

V

P

N

E

K

I

K

P

A

D

P

A
x
C

E

Y

T

R

C
|
C

A

C

R

F

V

K

G

G

V

I

V

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

S

V

M

A

A

Q

A

A

L

G

E

E

V

A

V

I

K

K

L

I

I

L

binding zinc ion: C184 (≠ A180), C187 (= C183)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 84% coverage: 3:208/244 of query aligns to 6:213/423 of 6yubA

query
sites
6yubA

F

L

S

S

E

K

D

E

E

E

V

I

E

E

R

R

Y

Y

A

S

R

R

H

Q

L

M

V

I

L

V

S

P

E

G

V

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

A

R

L

L

K

M

R

N

A

A

R

K

V

V

L

L

I

I

V

I

G

G

A

A

G

G

V

L

G

G

S

C

P

P

A

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

A

T

L

I

G

G

L

I

I

V

D

D

D

G

D

D

V

S

V

V

G

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

A

F

H

S

A

T

T

A

K

Q

R

V

V

G

G

R

M

S

L

K

K

V

V

E

D

A

S

A

L

A

I

E

T

R

H

L

L

V

I

G

E

L

I

N

N

P

P

H

L

V

P

A

V

I

Y

E

V

T

P

F

Y

A

R

E

F

R

D

I

L

T

T

A

P

E

Q

N

N

A

A

A

Q

R

I

I

I

E

K

A

P

F

W

D

D

I

V

V

I

L

L

D

D

G

C

T

T

D

D

D

N

F

P

Q

A

T

T

R

R

L

Y

W

L

V

I

N

S

A

D

A

V

C

C

V

V

A

L

G

G

K

K

P

P

L

L

V

V

S

S

G

A

A

A

L

S

G

V

R

Q

W

K

N

S

G

G

Q

Q

V

L

G

I

V

V

F

L

S

N

G

C

Q

P

P

P

C

T

Y

P

Q

Q

C

G

L

V

V

V

P

N

E

K

I

K

P

A

D

P

A
x
C

E

Y

T

R

C
|
C

-

C

-

F

A

K

R

K

V

P

G

G

V

I

V

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

S

V

M

A

A

Q

A

A

L

G

E

E

V

A

V

I

K

K

L

I

I

L

binding zinc ion: C183 (≠ A180), C186 (= C183)

Sites not aligning to the query:

binding zinc ion: 266, 269

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
27% identity, 98% coverage: 7:244/244 of query aligns to 88:338/338 of 3h5nA

query
sites
3h5nA

E

E

V

N

E

N

R

R

Y

Y

A

S

R

R

H

N

L

F

V

L

L

H

S

Y

E

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

A

K

L

L

K

K

R

N

A

A

R

K

V

V

L

V

I

I

V

L

G

G

A

C

G
|
G

G

G

V

I

G

G

S

N

P

H

A

V

A

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

A

E

L

I

G

I

L

L

I

I

D
|
D

D

N

D

D

V

Q

V

I

G

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

A

L

F

F

S

S

T

E

A

D

Q

D

V

V

G

G

R

K

S

N

K
|
K

V

T

E

E

A

V

A

I

A

K

E

R

R

E

L

L

V

L

G

K

L

R

N

N

P

S

H

E

V

I

A

S

I

V

E

S

T

E

F

I

A

A

E

L

R
x
N

I
|
I

T

N

A

D

E

Y

N

T

A

D

A

L

A

H

R

K

I

V

E

P

A

E

F

A

D

D

I

I

-

W

V

V

L

V

D

S

G
x
A

T
x
D

D
x
H

D

P

F

F

Q

N

T

L

R

I

L

N

W

W

V

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

G

N

K

Q

P

P

L

Y

V

I

S

N

G

A

A

G

L

-

G

-

R

-

W

Y

N

V

G

N

Q

D

V

I

G

A

V

V

F

F

-

G

-

P

-

L

-

Y

-

V

S

P

G

G

Q

K

P

T

-

G

C
|
C

Y

Y

Q

E

C
|
C

L

Q

V

K

P

K

E

E

I

N

P

I

P

D

D

H

A

K

-

I

-

K

-

L

-

I

E

N

T

S

C

R

A

F

R

K

V

P

G

A

V

T

V

F

G

A

A

P

L

V

T

N

G

N

V

V

I

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

V

V

V

I

K

K

L

F

I

I

T

G

G

K

A

Y

G

S

E

E

-

P

-

L

A

S

L

L

T

N

G

K

R

R

L

I

M

G

L

I

Y

W

D

S

G

D

L

-

A

E

G

I

S

K

A

I

R

H

T

S

V

Q

R

N

V

M

A

G

A

R

D

S

P

P

E

V

C
|
C

P

S

V

V

C
|
C

G

G

active site: R157 (= R72)
binding adenosine-5'-triphosphate: G124 (= G39), D146 (= D61), N154 (= N69), R157 (= R72), Q158 (= Q73), K170 (= K85), N193 (≠ R108), I194 (= I109), A213 (≠ G127), D214 (≠ T128), H215 (≠ D129)
binding zinc ion: C257 (= C168), C260 (= C171), C334 (= C240), C337 (= C243)

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
27% identity, 98% coverage: 7:244/244 of query aligns to 88:338/339 of 3h9jA

query
sites
3h9jA

E

E

V

N

E

N

R

R

Y

Y

A

S

R

R

H

N

L

F

V

L

L

H

S

Y

E

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

A

K

L

L

K

K

R

N

A

A

R

K

V

V

L

V

I

I

V

L

G

G

A

C

G
|
G

V

I

G

G

S

N

P

H

A

V

A

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

A

E

L

I

G

I

L

L

I

I

D
|
D

D

N

D
|
D

V

Q

V

I

G

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

A

L

F

F

S

S

T

E

A

D

Q

D

V

V

G

G

R

K

S

N

K
|
K

V

T

E

E

A

V

A

I

A

K

E

R

R

E

L

L

V

L

G

K

L

R

N

N

P

S

H

E

V

I

A

S

I

V

E

S

T

E

F

I

A

A

E

L

R
x
N

I
|
I

T

N

A

D

E

Y

N

T

A

D

A

L

A

H

R

K

I

V

E

P

A

E

F

A

D

D

I

I

-

W

V

V

L

V

D

S

G
x
A

T
x
D

D
x
H

D

P

F

F

Q

N

T

L

R

I

L

N

W

W

V

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

G

N

K

Q

P

P

L

Y

V

I

S

N

G

A

A

G

L

-

G

-

R

-

W

Y

N

V

G

N

Q

D

V

I

G

A

V

V

F

F

-

G

-

P

-

L

-

Y

-

V

S

P

G

G

Q

K

P

T

-

G

C
|
C

Y

Y

Q

E

C

C

L

Q

V

K

P

K

E

E

I

N

P

I

P

D

D

H

A

K

-

I

-

K

-

L

-

I

E

N

T

S

C

R

A

F

R

K

V

P

G

A

V

T

V

F

G

A

A

P

L

V

T

N

G

N

V

V

I

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

V

V

V

I

K

K

L

F

I

I

T

G

G

K

A

Y

G

S

E

E

-

P

-

L

A

S

L

L

T

N

G

K

R
|
R

L

I

M
x
G

L

I

Y

W

D

S

G

D

L

-

A

E

G

I

S

K

A

I

R

H

T

S

V

Q

R

N

V

M

A

G

A

R

D

S

P

P

E

V

C
|
C

P

S

V

V

C
|
C

G

G

active site: R157 (= R72)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G39), G125 (= G40), D146 (= D61), D148 (= D63), R157 (= R72), K170 (= K85), N193 (≠ R108), I194 (= I109), A213 (≠ G127), D214 (≠ T128), H215 (≠ D129)
binding zinc ion: C257 (= C168), C334 (= C240), C337 (= C243)
binding : R313 (= R218), G315 (≠ M220)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
27% identity, 98% coverage: 7:244/244 of query aligns to 88:339/340 of 3h5rA

query
sites
3h5rA

E

E

V

N

E

N

R

R

Y

Y

A

S

R

R

H

N

L

F

V

L

L

H

S

Y

E

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

A

K

L

L

K

K

R

N

A

A

R

K

V

V

L

V

I

I

V

L

G

G

A

C

G

G

V
x
I

G

G

S

N

P

H

A

V

A

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

A

E

L

I

G

I

L

L

I

I

D

D

D

N

D

D

V

Q

V

I

G

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

A

L

F

F

S

S

T

E

A

D

Q

D

V

V

G

G

R

K

S

N

K

K

V

T

E

E

A

V

A

I

A

K

E

R

R

E

L

L

V

L

G

K

L

R

N

N

P

S

H

E

V

I

A

S

I

V

E

S

T

E

F

I

A

A

E

L

R

N

I

I

T

N

A

D

E

Y

N

T

A

D

A

L

A

H

R

K

I

V

E

P

A

E

F

A

D

D

I

I

-

W

V

V

L

V

D
x
S

G
x
A

T

D

D

H

D

P

F

F

Q

N

T

L

R

I

L

N

W

W

V

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

G

N

K

Q

P

P

L

Y

V

I

S

N

G
x
A

A
x
G

L

-

G

-

R

-

W
x
Y

N

V

G

N

Q

D

V

I

G

A

V
|
V

F

F

-

G

-

P

-

L

-

Y

-

V

S

P

G

G

Q

K

P

T

-

G

C
|
C

Y

Y

Q

E

C
|
C

L

Q

V

K

P

V

E

K

I

E

P

N

P

I

D

D

A

H

E

K

-

I

-

K

-

L

-

I

-

N

T

S

C

R

A

F

R

K

V
x
P

G

A

V

T

V

F

G

A

A

P

L

V

T

N

G

N

V

V

I

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

V

V

V

I

K

K

L

F

I

I

T

G

G

K

A

Y

G

S

E

E

-

P

-

L

A

S

L

L

T

N

G

K

R
|
R

L

I

M
x
G

L

I

Y

W

D

S

G

D

L

-

A

E

G

I

S

K

A

I

R

H

T

S

V

Q

R

N

V

M

A

G

A

R

D

S

P

P

E

V

C
|
C

P

S

V

V

C
|
C

G

G

active site: R157 (= R72)
binding zinc ion: C257 (= C168), C260 (= C171), C335 (= C240), C338 (= C243)
binding : I126 (≠ V41), S212 (≠ D126), A213 (≠ G127), A237 (≠ G151), G238 (≠ A152), Y239 (≠ W156), V245 (= V162), P280 (≠ V186), R314 (= R218), G316 (≠ M220)

Sites not aligning to the query:

binding : 10, 14, 26

3h5aC Crystal structure of e. Coli mccb (see paper)
26% identity, 98% coverage: 7:244/244 of query aligns to 88:347/358 of 3h5aC

query
sites
3h5aC

E

E

V

N

E

N

R

R

Y

Y

A

S

R

R

H

N

L

F

V

L

L

H

S

Y

E

Q

V

S

G

Y

G

G

P

A

G

N