SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066553586.1 NCBI__GCF_001636925.1:WP_066553586.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 89% coverage: 6:252/276 of query aligns to 2:248/267 of 2hk9B

query
sites
2hk9B

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binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ A72), G130 (= G137), G133 (= G140), A134 (= A141), N153 (= N160), R154 (= R161), T155 (≠ S162), K158 (≠ R165), T188 (≠ L196), S189 (= S197), V190 (= V198), I214 (≠ M216), M238 (= M242), L239 (= L243)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S23), S21 (= S25), N64 (= N69), T66 (= T71), K70 (= K75), N91 (= N96), D106 (= D111), Y216 (= Y218), L239 (= L243), Q242 (= Q246)

2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 89% coverage: 6:252/276 of query aligns to 2:248/269 of 2hk9A

query
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2hk9A

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binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ A72), G132 (= G139), G133 (= G140), A134 (= A141), N153 (= N160), R154 (= R161), T155 (≠ S162), T188 (≠ L196), S189 (= S197), V190 (= V198)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S23), S21 (= S25), N64 (= N69), K70 (= K75), N91 (= N96), D106 (= D111), Y216 (= Y218), L239 (= L243), Q242 (= Q246)

O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
35% identity, 89% coverage: 6:252/276 of query aligns to 2:248/269 of O67049

query
sites
O67049

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SLS 19:21 (= SLS 23:25) binding shikimate
D82 (≠ A87) binding NADP(+)
N91 (= N96) binding shikimate
D106 (= D111) binding shikimate
GAGGA 130:134 (= GAGGA 137:141) binding NADP(+)
I214 (≠ M216) binding NADP(+)
Y216 (= Y218) binding shikimate
G235 (= G239) binding NADP(+)
Q242 (= Q246) binding shikimate

1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
37% identity, 86% coverage: 15:251/276 of query aligns to 6:249/271 of 1nytA

query
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1nytA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K75), D102 (= D111), G128 (= G139), G129 (= G140), A130 (= A141), N149 (= N160), R150 (= R161), T151 (≠ S162), R154 (= R165), T188 (≠ L196), S189 (= S197), S190 (≠ V198), M213 (= M216), G237 (= G239), M240 (= M242), L241 (= L243)

P15770 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Escherichia coli (strain K12) (see paper)
37% identity, 86% coverage: 15:251/276 of query aligns to 6:249/272 of P15770

query
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P15770

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A

L

G

D

A

C

E

A

V

V

R

T

I

I

V

T

N
|
N

R
|
R

S
x
T

P
x
V

E
x
S

R
|
R

A

A

Q

E

A

E

L

L

A

A

A

K

D

L

L

F

G

A

P

H

S

T

V

G

R

S

V

I

M

Q

A

A

-

L

-

S

-

M

-

D

-

E

-

L

A

E

G

G

D

H

A

E

F

F

D

D

G

-

A

-

L

L

V

I

I

I

N

N

A

A

L

T

S

S

V

S

A

G

-

I

-

S

-

G

-

D

-

I

P

P

S

A

I

I

D

P

F

S

D

S

R

L

I

I

A

H

P

P

G

G

T

I

V

Y

V

C

M

Y

D

D

M
|
M

T

F

Y

Y

K

Q

P

K

L

G

A

K

T

T

P

P

F

F

L

L

T

A

A

W

A

C

R

E

E

Q

R

R

G

G

-

S

L

K

R

R

T

N

V

A

D

D

G
|
G

L

L

A

G

M

M

L

L

I

V

G

A

Q

Q

A

A

P

H

S

A

F

F

GAGGA 126:130 (= GAGGA 137:141) binding NADP(+)
NRTVSR 149:154 (≠ NRSPER 160:165) binding NADP(+)
M213 (= M216) binding NADP(+)
G237 (= G239) binding NADP(+)

1p77A Crystal structure of shikimate dehydrogenase (aroe) from haemophilus influenzae (see paper)
34% identity, 89% coverage: 15:260/276 of query aligns to 6:251/265 of 1p77A

query
sites
1p77A

I

V

A

W

G

G

N

N

P

P

V

I

A

A

H

Q

S

S

L

K

S

S

P

P

V

L

I

I

H

Q

N

N

A

K

W

L

L

A

A

A

A

Q

G

T

V

H

I

Q

D

T

G

M

A

E

Y

Y

V

I

P

A

F

-

A

K

P

L

A

G

D

D

A

L

A

D

G

A

F

F

E

E

S

Q

L

Q

V

L

A

L

A

A

G

F

R

F

A

E

G

E

L

G

I

A

M

K

G

G

L

C

N

N

V

I

T

T

A

S

P

P

F

F

K

K

E

E

Q

R

A

A

F

Y

A

Q

L

L

A

A

D

D

Q

E

A

Y

T

S

A

Q

A

R

A

A

R

K

L

L

T

A

S

E

S

A

A

C

N

N

I

T

L

L

-

K

Q

K

F

L

E

D

N

D

G

G

R

K

V

L

L

Y

A

A

D

D

S

N

S

T

D

D

G

G

I

I

G

G

L

L

M

V

S

T

G

D

L

L

K

-

E

-

Q

Q

A

R

P

L

D

N

L

W

D

L

V

R

A

P

G

N

R

Q

P

H

V

V

V

L

M

I

L

L

G
|
G

A

A

G
|
G

A
|
A

A

T

R

K

A

G

G

V

S

L

G

L

A

P

L

L

V

L

E

Q

A

A

G

Q

A

Q

E

N

V

I

R

V

I

L

V

A

N
|
N

R
|
R

S
x
T

P

F

E

S

R
x
K

A

T

Q

K

A

E

L

L

A

A

A

E

D

R

L

F

G

Q

P

P

S

Y

V

G

R

N

V

I

M

Q

A

A

A

V

G

S

-

M

-

D

-

S

-

I

-

P

-

L

D

Q

A

T

F

Y

D

D

G

-

A

-

L

L

V

V

I

I

N

N

A

A

L
x
T

S
|
S

V
x
A

A

-

P

-

S

S

I

V

D

D

F

A

D

E

R

I

I

L

A

K

P

L

G

G

T

S

V

A

V

F

M

Y

D

D

M

M

T

Q

Y

Y

-

A

K

K

P

G

L

T

A

D

T

T

P

P

F

F

L

I

T

A

A

L

A

C

R

K

E

S

R

L

G

G

L

L

R

T

T

N

V

V

-

S

D

D

G

G

L

F

A

G

M

M

L

L

I

V

G

A

Q

Q

A

A

P

H

S

S

F

F

E

H

A

-

L

L

F

W

R

R

R

G

A

V

P

M

P

P

binding 2'-monophosphoadenosine-5'-diphosphate: G126 (= G137), G128 (= G139), G129 (= G140), A130 (= A141), N149 (= N160), R150 (= R161), T151 (≠ S162), K154 (≠ R165), T188 (≠ L196), S189 (= S197), A190 (≠ V198)

P43876 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
33% identity, 89% coverage: 15:260/276 of query aligns to 6:258/272 of P43876

query
sites
P43876

I

V

A

W

G

G

N

N

P

P

V

I

A

A

H

Q

S

S

L

K

S

S

P

P

V

L

I

I

H

Q

N

N

A

K

W

L

L

A

A

A

A

Q

G

T

V

H

I

Q

D

T

G

M

A

E

Y

Y

V

I

P

A

F

-

A

K

P

L

A

G

D

D

A

L

A

D

G

A

F

F

E

E

S

Q

L

Q

V

L

A

L

A

A

G

F

R

F

A

E

G

E

L

G

I

A

M

K

G

G

L

C

N

N

V

I

T

T

A

S

P

P

F

F

K

K

E

E

Q

R

A

A

F

Y

A

Q

L

L

A

A

D

D

Q

E

A

Y

T

S

A

Q

A

R

A

A

R

K

L

L

T

A

S

E

S

A

A

C

N

N

I

T

L

L

-

K

Q

K

F

L

E

D

N

D

G

G

R

K

V

L

L

Y

A

A

D

D

S

N

S

T

D

D

G

G

I

I

G

G

L

L

M

V

S

T

G

D

L

L

K

-

E

-

Q

Q

A

R

P

L

D

N

L

W

D

L

V

R

A

P

G

N

R

Q

P

H

V

V

V

L

M

I

L

L

G
|
G

A
|
A

G
|
G

A
|
A

A

T

R

K

A

G

G

V

S

L

G

L

A

P

L

L

V

L

E

Q

A

A

G

Q

A

Q

E

N

V

I

R

V

I

L

V

A

N
|
N

R
|
R

S
x
T

P
x
F

E
x
S

R
x
K

A

T

Q

K

A

E

L

L

A

A

A

E

D

R

L

F

G

Q

P

P

S

Y

V

G

R

N

V

I

M

Q

A

A

A

V

G

S

-

M

-

D

-

S

-

I

-

P

-

L

D

Q

A

T

F

Y

D

D

G

-

A

-

L

L

V

V

I

I

N

N

A

A

L

T

S
|
S

V

A

-

G

-

L

-

S

-

G

A

T

P

A

S

S

I

V

D

D

F

A

D

E

R

I

I

L

A

K

P

L

G

G

T

S

V

A

V

F

M

Y

D

D

M

M

T

Q

Y

Y

-

A

K

K

P

G

L

T

A

D

T

T

P

P

F

F

L

I

T

A

A

L

A

C

R

K

E

S

R

L

G

G

L

L

R

T

T

N

V

V

-

S

D

D

G

G

L

F

A

G

M

M

L

L

I

V

G

A

Q

Q

A

A

P

H

S

S

F

F

E

H

A

-

L

L

F

W

R

R

R

G

A

V

P

M

P

P

GAGGA 126:130 (= GAGGA 137:141) binding NADP(+)
NRTFSK 149:154 (≠ NRSPER 160:165) binding NADP(+)
S189 (= S197) binding NADP(+)

Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
32% identity, 89% coverage: 6:252/276 of query aligns to 2:260/282 of Q58484

query
sites
Q58484

I

I

T

N

G

A

A

K

A

T

M

K

V

V

A

I

G

G

I

L

A

I

G

G

N

H

P

P

V

V

A

E

H

H

S

S

L

F

S

S

P

P

V

I

I

M

H

H

N

N

A

A

W

A

L

F

A

K

A

D

G

K

V

G

I

L

D

N

G

Y

A

V

Y

Y

V

V

P

A

F

F

A

D

P

V

A

L

D

-

A

P

A

E

G

N

F

L

E

K

S

Y

L

V

V

I

A

D

A

G

G

A

R

K

A

A

G

L

L

G

I

I

M

V

G

G

L

F

N

N

V

V

T

T

A

I

P

P

F

H

K

K

E

I

Q

E

A

I

F

M

A

K

L

Y

A

L

D

D

Q

E

A

I

T

D

A

K

A

D

A

A

R

Q

L

L

T

I

S

G

S

A

A

V

N

N

I

T

L

I

Q

K

F

I

E

E

N

D

G

G

R

K

V

A

L

I

A

G

D

Y

S

N

S

T

D

D

G

G

I

I

G

G

L

A

M

R

S

M

G

A

L

L

K

E

E

E

Q

E

A

I

P

G

D

R

L

-

D

-

V

V

A

K

G

D

R

K

P

N

V

I

V

V

M

I

L

Y

G
|
G

A
|
A

G
|
G

A
|
A

A

A

R

R

A

A

G

V

S

A

G

F

A

E

L

L

V

A

E

K

A

D

G

N

A

-

E

N

V

I

R

I

I

I

V

A

N
|
N

R
|
R

S
x
T

P
x
V

E
|
E

R
x
K

A

A

Q

E

A

A

L

L

A

A

A

K

D

E

L

I

-

A

-

E

-

K

-

L

-

N

-

K

-

F

G

G

P

E

S

E

V

V

R

K

V

F

M

S

A

G

A

L

G

D

D

V

A

D

F

L

D

D

G

G

A

V

A

D

L

I

V

I

I

I

N

N

A

A

-
x
T

-

P

-

I

-

G

-
x
M

-

Y

-

P

-

N

L

I

S

D

V

V

A

E

P

P

S

I

I

V

D

K

F

A

D

E

R

K

I

L

A

R

P

E

G

D

T

M

V

V

M

M

D

D

M
x
L

T

I

Y

Y

K

N

P

P

L

L

A

E

T

T

P

V

F

L

L

L

T

K

A

E

A

A

R

K

E

K

R

V

G

N

L

A

R

K

T

T

V

I

D

N

G

G

L

L

A

G

M

M

L

L

I

I

G

Y

Q

Q

A

G

A

A

P

V

S

A

F

F

E

K

GAGGA 130:134 (= GAGGA 137:141) binding NADP(+)
NRTVEK 152:157 (≠ NRSPER 160:165) binding NADP(+)
T196 (vs. gap) binding NADP(+)
M200 (vs. gap) binding NADP(+)
L224 (≠ M216) binding NADP(+)

1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 89% coverage: 6:252/276 of query aligns to 7:265/287 of 1nvtB

query
sites
1nvtB

I

I

T

N

G

A

A

K

A

T

M

K

V

V

A

I

G

G

I

L

A

I

G

G

N

H

P

P

V

V

A
x
E

H
|
H

S

S

L

F

S

S

P

P

V

I

I

M

H

H

N

N

A

A

W

A

L

F

A

K

A

D

G

K

V

G

I

L

D

N

G

Y

A

V

Y

Y

V

V

P

A

F

F

A

D

P

V

A

L

D

-

A

P

A

E

G

N

F

L

E

K

S

Y

L

V

V

I

A

D

A

G

G

A

R

K

A

A

G

L

L

G

I

I

M

V

G

G

L

F

N

N

V

V

T

T

A
x
I

P

P

F

H

K
|
K

E

I

Q

E

A

I

F

M

A

K

L

Y

A

L

D

D

Q

E

A

I

T

D

A

K

A

D

A

A

R

Q

L

L

T

I

S

G

S

A

A

V

N

N

I

T

L

I

Q

K

F

I

E

E

N

D

G

G

R

K

V

A

L

I

A

G

D

Y

S

N

S

T

D
|
D

G

G

I

I

G

G

L

A

M

R

S

M

G

A

L

L

K

E

E

E

Q

E

A

I

P

G

D

R

L

-

D

-

V

V

A

K

G

D

R

K

P

N

V

I

V

V

M

I

L

Y

G
|
G

A

A

G
|
G

A
|
A

A

A

R

R

A

A

G

V

S

A

G

F

A

E

L

L

V

A

E

K

A

D

G

N

A

-

E

N

V

I

R

I

I

I

V

A

N
|
N

R
|
R

S
x
T

P

V

E

E

R
x
K

A

A

Q

E

A

A

L

L

A

A

A

K

D

E

L

I

-

A

-

E

-

K

-

L

-

N

-

K

-

F

G

G

P

E

S

E

V

V

R

K

V

F

M

S

A

G

A

L

G

D

D

V

A

D

F

L

D

D

G

G

A

V

A

D

L

I

V

I

I

I

N

N

A
|
A

-
x
T

-
x
P

-
x
I

-

G

-
x
M

-

Y

-

P

-

N

L

I

S

D

V

V

A

E

P

P

S

I

I

V

D

K

F

A

D

E

R

K

I

L

A

R

P

E

G

D

T

M

V

V

M

M

D

D

M
x
L

T

I

Y
|
Y

K

N

P

P

L

L

A

E

T

T

P

V

F

L

L

L

T

K

A

E

A

A

R

K

E

K

R

V

G

N

L

A

R

K

T

T

V

I

D

N

G

G

L

L

A

G

M
|
M

L
|
L

I

I

G

Y

Q

Q

A

G

A

A

P

V

S

A

F

F

E

K

active site: K75 (= K75), D111 (= D111)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ A72), G135 (= G137), G137 (= G139), G138 (= G140), A139 (= A141), N157 (= N160), R158 (= R161), T159 (≠ S162), K162 (≠ R165), A200 (= A195), T201 (vs. gap), P202 (vs. gap), I203 (vs. gap), M205 (vs. gap), L229 (≠ M216), Y231 (= Y218), M255 (= M242), L256 (= L243)
binding zinc ion: E22 (≠ A21), H23 (= H22)

1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
32% identity, 89% coverage: 6:252/276 of query aligns to 7:265/287 of 1nvtA

query
sites
1nvtA

I

I

T

N

G

A

A

K

A

T

M

K

V

V

A

I

G

G

I

L

A

I

G

G

N

H

P

P

V

V

A

E

H

H

S

S

L

F

S

S

P

P

V

I

I

M

H

H

N

N

A

A

W

A

L

F

A

K

A

D

G

K

V

G

I

L

D

N

G

Y

A

V

Y

Y

V

V

P

A

F

F

A

D

P

V

A

L

D

-

A

P

A

E

G

N

F

L

E

K

S

Y

L

V

V

I

A

D

A

G

G

A

R

K

A

A

G

L

L

G

I

I

M

V

G

G

L

F

N

N

V

V

T

T

A

I

P

P

F

H

K
|
K

E

I

Q

E

A

I

F

M

A

K

L

Y

A

L

D

D

Q

E

A

I

T

D

A

K

A

D

A

A

R

Q

L

L

T

I

S

G

S

A

A

V

N

N

I

T

L

I

Q

K

F

I

E

E

N

D

G

G

R

K

V

A

L

I

A

G

D

Y

S

N

S

T

D
|
D

G

G

I

I

G

G

L

A

M

R

S

M

G

A

L

L

K

E

E

E

Q

E

A

I

P

G

D

R

L

-

D

-

V

V

A

K

G

D

R

K

P

N

V

I

V

V

M

I

L

Y

G
|
G

A

A

G

G

A
|
A

A

A

R

R

A

A

G

V

S

A

G

F

A

E

L

L

V

A

E

K

A

D

G

N

A

-

E

N

V

I

R

I

I

I

V

A

N
|
N

R
|
R

S
x
T

P

V

E

E

R
x
K

A

A

Q

E

A

A

L

L

A

A

A

K

D

E

L

I

-

A

-

E

-

K

-

L

-

N

-

K

-

F

G

G

P

E

S

E

V

V

R

K

V

F

M

S

A

G

A

L

G

D

D

V

A

D

F

L

D

D

G

G

A

V

A

D

L

I

V

I

I

I

N

N

A
|
A

-
x
T

-
x
P

-
x
I

-

G

-
x
M

-

Y

-

P

-

N

L

I

S

D

V

V

A

E

P

P

S

I

I

V

D

K

F

A

D

E

R

K

I

L

A

R

P

E

G

D

T

M

V

V

M

M

D

D

M
x
L

T

I

Y
|
Y

K

N

P

P

L

L

A

E

T

T

P

V

F

L

L

L

T

K

A

E

A

A

R

K

E

K

R

V

G

N

L

A

R

K

T

T

V

I

D

N

G
|
G

L

L

A

G

M
|
M

L
|
L

I

I

G

Y

Q

Q

A

G

A

A

P

V

S

A

F

F

E

K

active site: K75 (= K75), D111 (= D111)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G137), A139 (= A141), N157 (= N160), R158 (= R161), T159 (≠ S162), K162 (≠ R165), A200 (= A195), T201 (vs. gap), P202 (vs. gap), I203 (vs. gap), M205 (vs. gap), L229 (≠ M216), Y231 (= Y218), G252 (= G239), M255 (= M242), L256 (= L243)

3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
28% identity, 93% coverage: 11:267/276 of query aligns to 1:248/258 of 3dooA

query
sites
3dooA

M

M

V

K

A

F

G

A

I

V

A

I

G

G

N

N

P

P

V

I

A

S

H

H

S
|
S

L

L

S
|
S

P

P

V

L

I

M

H

H

N

H

A

A

W

N

L

F

A

Q

A

S

G

L

V

N

I

L

D

E

G

N

A

T

Y

Y

V

E

P

A

F

I

-

N

A

V

P

P

A

V

D

N

A

Q

A

-

G

-

F

F

E

Q

S

D

L

I

V

K

A

K

A

I

G

I

R

S

A

E

G

K

L

S

I

I

M

D

G

G

L

F

N
|
N

V

V

T
|
T

A

I

P

P

F

H

K
|
K

E

E

Q

R

A

I

F

I

A

P

L

Y

A

L

D

D

Q

D

A

I

T

N

A

E

A

Q

A

A

R

K

L

S

T

V

S

G

S

A

A

V

N
|
N

I

T

L

V

Q

L

F

V

E

K

N

D

G

G

R

K

V

W

L

I

A

G

D

Y

S

N

S

T

D
|
D

G

G

I

I

G

G

L

Y

M

V

S

N

G

G

L

L

K

K

E

Q

Q

I

A

Y

P

E

D

G

L

I

D

E

V

D

A

A

G

Y

R

-

P

-

V

I

V

L

M

I

L

L

G

G

A

A

G

G

A

A

A

S

R

K

A

G

G

I

S

A

G

N

A

E

L

L

V

Y

E

K

-

I

A

V

G

R

A

P

E

T

V

L

R

T

I

V

V

A

N

N

R

R

S

T

P

M

E

S

R

R

A

F

Q

N

A

N

L

W

A

S

A

L

D

N

L

I

G

N

P

-

S

K

V

I

R

N

V

L

M

S

A

H

A

A

G

E

D

S

A

H

F

L

D

D

G

E

A

F

A

D

L

I

V

I

I

I

N

N

A

T

L

-

S

-

V

-

A

T

P

P

S

V

I

I

D

S

F

L

D

N

R

R

I

L

A

A

P

S

G

H

T

T

V

L

V

V

M

S

D

D

M

I

T

V

Y

Y

K

N

P

P

L

Y

A

K

T

T

P

P

F

I

L

L

T

I

A

E

A

A

R

E

E

Q

R

R

G

G

L

N

R

P

T

I

V

Y

D

N

G

G

L

L

A

D

M

M

L
x
F

I

V

G

H

Q
|
Q

A

G

A

A

P

E

S

S

F

F

E

K

A

I

L

W

F

T

R

N

R

L

A

E

P

P

P

-

L

-

D

D

L

I

R

K

A

A

L

M

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S23), S15 (= S25), N58 (= N69), T60 (= T71), K64 (= K75), N85 (= N96), D100 (= D111), F227 (≠ L243), Q230 (= Q246)

3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
29% identity, 97% coverage: 3:269/276 of query aligns to 6:291/291 of 3tozA

query
sites
3tozA

T

T

H

E

R

R

I

I

T

T

G

G

A

H

A

T

M

E

V

L

A

I

G

G

I

L

A

I

G

A

N

T

P

P

V

I

A

R

H

H

S

S

L

L

S

S

P

P

V

T

I

M

H

H

N

N

A

E

W

A

L

F

A

A

A

K

G

L

V

G

I

L

D

D

G

Y

A

V

Y

Y

V

L

P

A

F

F

A

E

P

V

A

G

D

D

A

K

A

E

G

-

F

L

E

K

S

D

L

V

V

V

A

Q

A

G

G

F

R

R

A

A

G

M

L

N

I

L

M

R

G

G

L

W

N

N

V

V

T

S

A

M

P

P

F

N

K

K

E

T

Q

N

A

I

F

H

A

K

L

Y

A

L

D

D

Q

K

A

L

T

S

A

P

A

A

R

E

L

L

T

V

S

G

S

A

A

V

N

N

I

T

L

V

Q

V

F

N

E

D

N

D

G

G

R

V

V

L

L

T

A

G

D

H

S

I

S

T

D

D

G

G

I

T

G

G

L

Y

M

M

S

R

G

A

L

L

K

K

E

E

Q

A

A

G

P

H

D

-

L

-

D

D

V

I

A

I

G

G

R

K

P

K

V

M

V

T

M

I

L

C

G
|
G

A
|
A

G
|
G

A
|
A

A

A

R

T

A

A

G

I

S

C

-

I

G

Q

A

A

L

A

V

L

E

D

A

G

G

V

A

K

E

E

V

I

R

S

I

I

V

F

N
|
N

R
|
R

S

K

P
x
D

-

D

-
x
F

-

Y

-

A

-

N

-

A

-

E

-

K

-

T

-

V

-

E

-

K

-

I

-

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

E

E

R

D

A

H

Q

E

A

Q

L

L

A

R

A

K

D

E

L

I

G

A

P

E

S

S

V

V

R

I

V

F

M

T

A

N

A

A

G
x
T

-
x
G

-
x
V

-

G

-
x
M

D

K

A

P

F
|
F

D

E

G

G

A

E

A

T

L

L

V

L

I

-

N

-

A

-

L

-

S

-

V

-

A

-

P

P

S

S

I

A

D

D

F

M

D

L

R

R

I

-

A

-

P

P

G

E

T

L

V

I

V

V

M

S

D

D

M
x
V

T

V

Y
|
Y

K

K

P

P

L

T

A

K

T

T

P

R

F

L

L

L

T

E

A

I

A

A

R

E

E

E

R

Q

G

G

L

C

R

Q

T

T

V

L

D

N

G
|
G

L

L

A

G

M
|
M

L
x
M

I

L

G

W

Q

Q

A

G

A

A

P

K

S

A

F

F

E

E

A

I

L

W

F

T

R

H

R

K

A

E

P

M

P

P

P

V

L

D

D

Y

L

I

R

K

A

E

L

I

L

L

M

F

binding nicotinamide-adenine-dinucleotide: G137 (= G137), A138 (= A138), G139 (= G139), G140 (= G140), A141 (= A141), N161 (= N160), R162 (= R161), D164 (≠ P163), F166 (vs. gap), T210 (≠ G183), G211 (vs. gap), V212 (vs. gap), M214 (vs. gap), F217 (= F186), V238 (≠ M216), Y240 (= Y218), G261 (= G239), M264 (= M242), M265 (≠ L243)

Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
29% identity, 97% coverage: 3:269/276 of query aligns to 6:291/291 of Q8Y9N5

query
sites
Q8Y9N5

T

T

H

E

R

R

I

I

T

T

G

G

A

H

A

T

M

E

V

L

A

I

G

G

I

L

A

I

G

A

N

T

P

P

V

I

A

R

H

H

S
|
S

L
|
L

S
|
S

P

P

V

T

I

M

H

H

N

N

A

E

W

A

L

F

A

A

A

K

G

L

V

G

I

L

D

D

G

Y

A

V

Y

Y

V

L

P

A

F

F

A

E

P

V

A

G

D

D

A

K

A

E

G

-

F

L

E

K

S

D

L

V

V

V

A

Q

A

G

G

F

R

R

A

A

G

M

L

N

I

L

M

R

G

G

L

W

N

N

V

V

T

S

A

M

P

P

F

N

K

K

E

T

Q

N

A

I

F

H

A

K

L

Y

A

L

D

D

Q

K

A

L

T

S

A

P

A

A

R

E

L

L

T

V

S

G

S

A

A

V

N

N

I

T

L

V

Q

V

F

N

E

D

N

D

G

G

R

V

V

L

L

T

A

G

D

H

S

I

S

T

D

D

G

G

I

T

G

G

L

Y

M

M

S

R

G

A

L

L

K

K

E

E

Q

A

A

G

P

H

D

-

L

-

D

D

V

I

A

I

G

G

R

K

P

K

V

M

V

T

M

I

L

C

G

G

A

A

G

G

A

A

R

T

A

A

G

I

S

C

-

I

G

Q

A

A

L

A

V

L

E

D

A

G

G

V

A

K

E

E

V

I

R

S

I

I

V

F

N
|
N

R
|
R

S
x
K

P
x
D

-

D

-

F

-

Y

-

A

-

N

-

A

-

E

-

K

-

T

-

V

-

E

-

K

-

I

-

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

E

E

R

D

A

H

Q

E

A

Q

L

L

A

R

A

K

D

E

L

I

G

A

P

E

S

S

V

V

R

I

V

F

M

T

A

N

A

A

G

T

-

G

-

V

-

G

-
x
M

D

K

A

P

F

F

D

E

G

G

A

E

A

T

L

L

V

L

I

-

N

-

A

-

L

-

S

-

V

-

A

-

P

P

S

S

I

A

D

D

F

M

D

L

R

R

I

-

A

-

P

P

G

E

T

L

V

I

V

V

M

S

D

D

M

V

T

V

Y

Y

K

K

P

P

L

T

A

K

T

T

P

R

F

L

L

L

T

E

A

I

A

A

R

E

E

E

R

Q

G

G

L

C

R

Q

T

T

V

L

D

N

G

G

L

L

A

G

M

M

L

M

I

L

G

W

Q

Q

A

G

A

A

P

K

S

A

F

F

E

E

A

I

L

W

F

T

R

H

R

K

A

E

P

M

P

P

P

V

L

D

D

Y

L

I

R

K

A

E

L

I

L

L

M

F

SLS 26:28 (= SLS 23:25) binding shikimate
NRKD 161:164 (≠ NRSP 160:163) binding NAD(+)
M214 (vs. gap) binding NADP(+)

3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
29% identity, 97% coverage: 3:269/276 of query aligns to 3:288/288 of 3tnlA

query
sites
3tnlA

T

T

H

E

R

R

I

I

T

T

G

G

A

H

A

T

M

E

V

L

A

I

G

G

I

L

A

I

G

A

N

T

P

P

V

I

A

R

H

H

S
|
S

L

L

S
|
S

P

P

V

T

I

M

H

H

N

N

A

E

W

A

L

F

A

A

A

K

G

L

V

G

I

L

D

D

G

Y

A

V

Y

Y

V

L

P

A

F

F

A

E

P

V

A

G

D

D

A

K

A

E

G

-

F

L

E

K

S

D

L

V

V

V

A

Q

A

G

G

F

R

R

A

A

G

M

L

N

I

L

M

R

G

G

L

W

N
|
N

V

V

T
x
S

A
x
M

P

P

F

N

K
|
K

E

T

Q

N

A

I

F

H

A

K

L

Y

A

L

D

D

Q

K

A

L

T

S

A

P

A

A

R

E

L

L

T

V

S

G

S

A

A

V

N
|
N

I

T

L

V

Q

V

F

N

E

D

N

D

G

G

R

V

V

L

L

T

A

G

D

H

S

I

S

T

D
|
D

G

G

I

T

G

G

L

Y

M

M

S

R

G

A

L

L

K

K

E

E

Q

A

A

G

P

H

D

-

L

-

D

D

V

I

A

I

G

G

R

K

P

K

V

M

V

T

M

I

L

C

G
|
G

A
|
A

G
|
G

A
|
A

A

A

R

T

A

A

G

I

S

C

-

I

G

Q

A

A

L

A

V

L

E

D

A

G

G

V

A

K

E

E

V

I

R

S

I

I

V

F

N
|
N

R
|
R

S

K

P
x
D

-

D

-
x
F

-

Y

-

A

-

N

-

A

-

E

-

K

-

T

-

V

-

E

-

K

-

I

-

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

-

Q

-

L

-

F

-

D

-

I

E

E

R

D

A

H

Q

E

A

Q

L

L

A

R

A

K

D

E

L

I

G

A

P

E

S

S

V

V

R

I

V

F

M

T

A

N

A

A

G
x
T

-

G

-
x
V

-

G

-
x
M

D

K

A

P

F
|
F

D

E

G

G

A

E

A

T

L

L

V

L

I

-

N

-

A

-

L

-

S

-

V

-

A

-

P

P

S

S

I

A

D

D

F

M

D

L

R

R

I

-

A

-

P

P

G

E

T

L

V

I

V

V

M

S

D

D

M
x
V

T

V

Y
|
Y

K

K

P

P

L

T

A

K

T

T

P

R

F

L

L

L

T

E

A

I

A

A

R

E

E

E

R

Q

G

G

L

C

R

Q

T

T

V

L

D

N

G

G

L

L

A

G

M
|
M

L
x
M

I

L

G

W

Q
|
Q

A

G

A

A

P

K

S

A

F

F

E

E

A

I

L

W

F

T

R

H

R

K

A

E

P

M

P

P

P

V

L

D

D

Y

L

I

R

K

A

E

L

I

L

L

M

F

binding nicotinamide-adenine-dinucleotide: M71 (≠ A72), G134 (= G137), A135 (= A138), G136 (= G139), G137 (= G140), A138 (= A141), N158 (= N160), R159 (= R161), D161 (≠ P163), F163 (vs. gap), T207 (≠ G183), V209 (vs. gap), M211 (vs. gap), F214 (= F186), V235 (≠ M216), Y237 (= Y218), M261 (= M242), M262 (≠ L243)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S23), S25 (= S25), N68 (= N69), S70 (≠ T71), K74 (= K75), N95 (= N96), D110 (= D111), Q265 (= Q246)

Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
33% identity, 89% coverage: 15:260/276 of query aligns to 10:261/278 of Q9KVT3

query
sites
Q9KVT3

I

V

A

F

G

G

N

N

P

P

V

I

A

N

H

H

S
|
S

L
x
K

S
|
S

P

P

V

F

I

I

H

H

N

T

A

L

W

F

-

A

-

R

-

Q

-

T

L

Q

A

Q

A

S

G

M

V

I

I

Y

D

T

G

A

A

Q

Y

C

V

V

P

P

F

V

A

D

P

G

A

F

D

T

A

E

A

A

G

A

F

K

E

H

S

F

L

F

V

A

A

Q

A

G

G

G

R

R

A

-

G

-

L

-

I

-

M

-

G

G

L

C

N

N

V

V

T

T

A

V

P

P

F

F

K

K

E

E

Q

E

A

A

F

Y

A

R

L

F

A

A

D

D

Q

R

A

L

T

T

A

E

A

R

A

A

R

R

L

L

T

A

S

G

S

A

A

V

N
|
N

I

T

L

L

-

K

Q

K

F

L

E

D

N

D

G

G

R

E

V

I

L

L

A

G

D

D

S

N

S

T

D
|
D

G

G

I

E

G

G

L

L

M

V

S

Q

G

D

L

L

K

L

E

A

Q

Q

A

-

P

-

D

Q

L

V

D

L

V

L

A

K

G

G

R

A

P

T

V

I

V

L

M

L

L

I

G

G

A

A

G

G

A

A

R

R

A

G

G

V

S

L

G

K

A

P

L

L

V

L

E

D

A

Q

G

Q

-

P

A

A

E

S

V

I

R

T

I

V

V

T

N
|
N

R
|
R

S
x
T

P
x
F

E
x
A

R
x
K

A

A

Q

E

A

Q

L

L

A

A

A

E

D

L

L

V

G

A

P

A

-

Y

-

G

S

E

V

V

R

K

V

A

M

Q

A

A

A

F

G

E

D

Q

A

L

F

K

D

Q

G

S

A

Y

A

D

L

V

V

I

I

I

N

N

A

S

L

T

S

S

V

A

A

S

-

L

-

D

-

G

-

E

-

L

P

P

S

A

I

I

D

D

F

P

D

V

R

I

I

F

A

S

P

S

G

R

T

S

V

V

V

C

M

Y

D

D

M

M

T

M

Y

Y

K

G

P

K

L

G

A

Y

T

T

P

V

F

F

L

N

T

Q

A

W

A

A

R

R

E

Q

R

H

G

G

L

C

-

A

R

Q

T

A

V

I

D

D

G

G

L

L

A

G

M

M

L

L

I

V

G

G

Q
|
Q

A

A

P

E

S

S

F

F

E

-

A

M

L

L

F

W

R

R

R

G

A

L

P

R

P

P

SKS 18:20 (≠ SLS 23:25) binding shikimate
N90 (= N96) binding shikimate
D106 (= D111) binding shikimate
NRTFAK 154:159 (≠ NRSPER 160:165) binding NADP(+)
Q248 (= Q246) binding shikimate

3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
33% identity, 86% coverage: 15:251/276 of query aligns to 6:249/272 of 3pgjA

query
sites
3pgjA

I

V

A

F

G

G

N

N

P

P

V

I

A

N

H

H

S
|
S

L

K

S
|
S

P

P

V

F

I

I

H

H

N

T

A

L

W

F

-

A

-

R

-

Q

-

T

L

Q

A

Q

A

S

G

M

V

I

I

Y

D

T

G

A

A

Q

Y

C

V

V

P

P

F

V

A

D

P

G

A

F

D

T

A

E

A

A

G

A

F

K

E

H

S

F

L

F

V

A

A

Q

A

G

G

G

R

R

A

-

G

-

L

-

I

-

M

-

G

G

L

C

N
|
N

V

V

T
|
T

A

V

P

P

F

F

K
|
K

E

E

Q

E

A

A

F

Y

A

R

L

F

A

A

D

D

Q

R

A

L

T

T

A

E

A

R

A

A

R

R

L

L

T

A

S

G

S

A

A

V

N
|
N

I

T

L

L

-

K

Q

K

F

L

E

D

N

D

G

G

R

E

V

I

L

L

A

G

D

D

S

N

S

T

D
|
D

G

G

I

E

G

G

L

L

M

V

S

Q

G

D

L

L

K

L

E

A

Q

Q

A

-

P

-

D

Q

L

V

D

L

V

L

A

K

G

G

R

A

P

T

V

I

V

L

M

L

L

I

G

G

A

A

G

G

A

A

R

R

A

G

G

V

S

L

G

K

A

P

L

L

V

L

E

D

A

Q

G

Q

-

P

A

A

E

S

V

I

R

T

I

V

V

T

N

N

R

R

S

T

P

F

E

A

R

K

A

A

Q

E

A

Q

L

L

A

A

A

E

D

L

L

V

G

A

P

A

-

Y

-

G

S

E

V

V

R

K

V

A

M

Q

A

A

A

F

G

E

D

Q

A

L

F

K

D

Q

G

S

A

Y

A

D

L

V

V

I

I

I

N

N

A

S

L

T

S

S

V

A

A

S

-

L

-

D

-

G

-

E

-

L

P

P

S

A

I

I

D

D

F

P

D

V

R

I

I

F

A

S

P

S

G

R

T

S

V

V

V

C

M

Y

D

D

M

M

T

M

Y

Y

K

G

P

K

L

G

A

Y

T

T

P

V

F

F

L

N

T

Q

A

W

A

A

R

R

E

Q

R

H

G

G

L

C

-

A

R

Q

T

A

V

I

D

D

G

G

L

L

A

G

M

M

L

L

I

V

G

G

Q
|
Q

A

A

P

E

S

S

F

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S23), S16 (= S25), N59 (= N69), T61 (= T71), K65 (= K75), N86 (= N96), D102 (= D111), Q244 (= Q246)

3phjA Shikimate 5-dehydrogenase (aroe) from helicobacter pylori in complex with 3-dehydroshikimate
34% identity, 87% coverage: 14:254/276 of query aligns to 7:237/255 of 3phjA

query
sites
3phjA

G

G

I

V

A

F

G

G

N

N

P

P

V

I

A

K

H

H

S
|
S

L

K

S
|
S

P

P

V

L

I

I

H

H

N

N

A

A

W

C

L

F

A

L

A

T

G

F

V

Q

I

K

D

E

-

L

-

R

-

F

-

L

G

G

A

H

Y

Y

V

H

P

P

-

I

F

L

A

L

P

P

A

L

D

E

A

S

A

H

G

I

F

K

E

S

S

E

L

F

V

L

A

H

A

L

G

G

R

-

A

-

G

-

L

-

I

L

M

S

G

G

L

A

N
|
N

V

V

T

T

A

L

P

P

F

F

K
|
K

E

E

Q

R

A

A

F

F

A

Q

L

V

A

C

D

D

Q

K

A

I

T

K

A

G

A

I

A

A

R

L

L

E

T

C

S

G

S

A

A

V

N
|
N

I

T

L

L

Q

V

F

L

E

E

N

N

G

D

R

E

V

L

L

V

A

G

D

Y

S

N

S

T

D
|
D

G

A

I

L

G

G

L

F

M

Y

S

L

G

S

L

L

K

K

E

Y

Q

Q

A

N

P

-

D

-

L

-

D

-

V

-

A

-

G

-

R

-

P

-

V

A

V

L

M

I

L

L