SitesBLAST
Comparing WP_066601421.1 NCBI__GCF_001046645.1:WP_066601421.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
44% identity, 92% coverage: 19:258/260 of query aligns to 17:256/258 of 1ey3A
- active site: A66 (= A68), M71 (≠ L73), S81 (≠ A83), L85 (≠ P87), G109 (≠ A111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ L227), F235 (= F237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E26), L26 (≠ R28), A28 (= A30), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), L85 (≠ P87), W88 (= W90), G109 (≠ A111), P131 (= P133), L135 (= L137), G140 (= G142)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
44% identity, 92% coverage: 19:258/260 of query aligns to 19:258/260 of 1dubA
- active site: A68 (= A68), M73 (≠ L73), S83 (≠ A83), L87 (≠ P87), G111 (≠ A111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ L227), F237 (= F237)
- binding acetoacetyl-coenzyme a: K26 (≠ E26), A27 (≠ K27), L28 (≠ R28), A30 (= A30), A66 (= A66), A68 (= A68), D69 (= D69), I70 (= I70), Y107 (≠ W107), G110 (= G110), G111 (≠ A111), E114 (= E114), P133 (= P133), E134 (= E134), L137 (= L137), G142 (= G142), F233 (= F233), F249 (= F249)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
44% identity, 92% coverage: 19:258/260 of query aligns to 49:288/290 of P14604
- E144 (= E114) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
44% identity, 92% coverage: 19:258/260 of query aligns to 18:252/254 of 2dubA
- active site: A67 (= A68), M72 (≠ L73), S82 (≠ A83), G105 (≠ A111), E108 (= E114), P127 (= P133), E128 (= E134), T133 (≠ I139), P135 (= P141), G136 (= G142), K221 (≠ L227), F231 (= F237)
- binding octanoyl-coenzyme a: K25 (≠ E26), A26 (≠ K27), L27 (≠ R28), A29 (= A30), A65 (= A66), A67 (= A68), D68 (= D69), I69 (= I70), K70 (≠ A71), G105 (≠ A111), E108 (= E114), P127 (= P133), E128 (= E134), G136 (= G142), A137 (= A143)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
44% identity, 92% coverage: 19:258/260 of query aligns to 19:256/258 of 1mj3A
- active site: A68 (= A68), M73 (≠ L73), S83 (≠ G80), L85 (= L82), G109 (≠ A111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ L227), F235 (= F237)
- binding hexanoyl-coenzyme a: K26 (≠ E26), A27 (≠ K27), L28 (≠ R28), A30 (= A30), A66 (= A66), G67 (= G67), A68 (= A68), D69 (= D69), I70 (= I70), G109 (≠ A111), P131 (= P133), E132 (= E134), L135 (= L137), G140 (= G142)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
41% identity, 92% coverage: 19:258/260 of query aligns to 19:258/260 of 2hw5C
- active site: A68 (= A68), M73 (≠ L73), S83 (≠ A83), L87 (≠ P87), G111 (≠ A111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ L227), F237 (= F237)
- binding crotonyl coenzyme a: K26 (≠ E26), A27 (≠ K27), L28 (≠ R28), A30 (= A30), K62 (≠ R62), I70 (= I70), F109 (≠ L109)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
40% identity, 92% coverage: 19:258/260 of query aligns to 16:255/255 of 3q0jC
- active site: A65 (= A68), M70 (≠ L73), T80 (≠ A83), F84 (≠ P87), G108 (≠ A111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (= L227), F234 (= F237)
- binding acetoacetyl-coenzyme a: Q23 (≠ E26), A24 (≠ K27), L25 (≠ R28), A27 (= A30), A63 (= A66), G64 (= G67), A65 (= A68), D66 (= D69), I67 (= I70), K68 (≠ A71), M70 (≠ L73), F84 (≠ P87), G107 (= G110), G108 (≠ A111), E111 (= E114), P130 (= P133), E131 (= E134), P138 (= P141), G139 (= G142), M140 (≠ A143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
40% identity, 92% coverage: 19:258/260 of query aligns to 16:255/255 of 3q0gC
- active site: A65 (= A68), M70 (≠ L73), T80 (≠ A83), F84 (≠ P87), G108 (≠ A111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (= L227), F234 (= F237)
- binding coenzyme a: L25 (≠ R28), A63 (= A66), I67 (= I70), K68 (≠ A71), Y104 (≠ W107), P130 (= P133), E131 (= E134), L134 (= L137)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
40% identity, 92% coverage: 19:258/260 of query aligns to 15:254/256 of 3h81A
- active site: A64 (= A68), M69 (≠ L73), T79 (≠ A83), F83 (≠ P87), G107 (≠ A111), E110 (= E114), P129 (= P133), E130 (= E134), V135 (≠ I139), P137 (= P141), G138 (= G142), L223 (= L227), F233 (= F237)
- binding calcium ion: F233 (= F237), Q238 (≠ K242)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
40% identity, 92% coverage: 19:258/260 of query aligns to 15:250/250 of 3q0gD
- active site: A64 (= A68), M69 (≠ L73), T75 (≠ A83), F79 (≠ P87), G103 (≠ A111), E106 (= E114), P125 (= P133), E126 (= E134), V131 (≠ I139), P133 (= P141), G134 (= G142), L219 (= L227), F229 (= F237)
- binding Butyryl Coenzyme A: F225 (= F233), F241 (= F249)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
37% identity, 94% coverage: 16:259/260 of query aligns to 13:258/259 of 5zaiC
- active site: A65 (= A68), F70 (≠ L73), S82 (≠ P85), R86 (vs. gap), G110 (≠ A111), E113 (= E114), P132 (= P133), E133 (= E134), I138 (= I139), P140 (= P141), G141 (= G142), A226 (≠ L227), F236 (= F237)
- binding coenzyme a: K24 (= K27), L25 (≠ R28), A63 (= A66), G64 (= G67), A65 (= A68), D66 (= D69), I67 (= I70), P132 (= P133), R166 (≠ P167), F248 (= F249), K251 (= K252)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
37% identity, 93% coverage: 16:257/260 of query aligns to 17:263/266 of O53561
- K135 (= K129) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 129:136, 50% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ N136) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
38% identity, 93% coverage: 16:257/260 of query aligns to 20:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
37% identity, 90% coverage: 27:259/260 of query aligns to 25:260/261 of 5jbxB
- active site: A67 (= A68), R72 (≠ L73), L84 (≠ A83), R88 (≠ P87), G112 (≠ A111), E115 (= E114), T134 (≠ P133), E135 (= E134), I140 (= I139), P142 (= P141), G143 (= G142), A228 (≠ L227), L238 (≠ F237)
- binding coenzyme a: R25 (≠ K27), R26 (= R28), A28 (= A30), A65 (= A66), D68 (= D69), L69 (≠ I70), K70 (≠ A71), L110 (= L109), G111 (= G110), T134 (≠ P133), E135 (= E134), L138 (= L137), R168 (≠ P167)
Sites not aligning to the query:
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
35% identity, 91% coverage: 17:252/260 of query aligns to 12:247/247 of 7borA
- active site: N63 (≠ A68), F68 (≠ L73), D77 (= D84), G81 (≠ P87), I105 (≠ A111), T108 (≠ E114), F128 (≠ E134), L133 (≠ I139), P135 (= P141), E136 (≠ G142), A222 (≠ L227), L232 (≠ F237)
- binding coenzyme a: D21 (≠ E26), K22 (= K27), A25 (= A30), S61 (≠ A66), I65 (= I70), V103 (≠ L109), F128 (≠ E134), L131 (= L137), F244 (= F249), R247 (≠ K252)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 93% coverage: 17:259/260 of query aligns to 13:256/257 of 6slbAAA
- active site: Q64 (≠ A68), F69 (≠ L73), L80 (= L82), N84 (≠ R86), A108 (= A111), S111 (≠ E114), A130 (≠ P133), F131 (≠ E134), L136 (≠ I139), P138 (= P141), D139 (≠ G142), A224 (≠ L227), G234 (≠ F237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R62), A62 (= A66), Q64 (≠ A68), D65 (= D69), L66 (≠ I70), Y76 (≠ T78), A108 (= A111), F131 (≠ E134), D139 (≠ G142)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 93% coverage: 17:259/260 of query aligns to 10:244/245 of 6slaAAA