SitesBLAST
Comparing WP_066608734.1 NCBI__GCF_001046645.1:WP_066608734.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3wy2A Crystal structure of alpha-glucosidase in complex with glucose (see paper)
51% identity, 98% coverage: 12:534/535 of query aligns to 4:533/535 of 3wy2A
- active site: D97 (= D105), R197 (= R205), D199 (= D207), E268 (= E271), H329 (= H333), D330 (= D334)
- binding beta-D-glucopyranose: D59 (= D67), Y62 (= Y70), H102 (= H110), R197 (= R205), D199 (= D207), E268 (= E271), H329 (= H333), D330 (= D334), R397 (= R401)
- binding glycerol: S101 (≠ A109), D169 (≠ Q177), V170 (≠ L178), P175 (= P183), R178 (≠ Q186), Q179 (≠ D187), F203 (≠ H211), Y204 (≠ S212), H206 (= H214), D240 (≠ S243), P244 (= P247), E280 (≠ A283)
- binding magnesium ion: D20 (= D28), R22 (≠ N30), V26 (≠ I34), D28 (= D36)
3wy1A Crystal structure of alpha-glucosidase (see paper)
51% identity, 98% coverage: 12:534/535 of query aligns to 4:533/535 of 3wy1A
- active site: D97 (= D105), R197 (= R205), D199 (= D207), E268 (= E271), H329 (= H333), D330 (= D334)
- binding glycerol: S101 (≠ A109), D169 (≠ Q177), V170 (≠ L178), F203 (≠ H211), Y204 (≠ S212), H206 (= H214)
- binding magnesium ion: D20 (= D28), D24 (= D32), V26 (≠ I34), D28 (= D36)
- binding (3R,5R,7R)-octane-1,3,5,7-tetracarboxylic acid: D59 (= D67), Y62 (= Y70), H102 (= H110), F144 (= F152), F163 (= F171), R197 (= R205), D199 (= D207), T200 (≠ A208), G225 (= G228), E268 (= E271), F294 (= F297), H329 (= H333), D330 (= D334), R397 (= R401)
3wy4A Crystal structure of alpha-glucosidase mutant e271q in complex with maltose (see paper)
51% identity, 98% coverage: 12:534/535 of query aligns to 4:533/535 of 3wy4A
- active site: D97 (= D105), R197 (= R205), D199 (= D207), Q268 (≠ E271), H329 (= H333), D330 (= D334)
- binding alpha-D-glucopyranose: D59 (= D67), Y62 (= Y70), H102 (= H110), I143 (≠ V151), F163 (= F171), D199 (= D207), T200 (≠ A208), G225 (= G228), Q268 (≠ E271), Q268 (≠ E271), F294 (= F297), H329 (= H333), D330 (= D334), R397 (= R401), R397 (= R401)
- binding glycerol: S101 (≠ A109), D169 (≠ Q177), V170 (≠ L178), F203 (≠ H211), Y204 (≠ S212), H206 (= H214), L224 (≠ T227), G225 (= G228), A226 (≠ K229), P227 (= P230), F294 (= F297), D330 (= D334), E374 (≠ Q378), K395 (≠ L399), G396 (≠ S400), G399 (= G403)
- binding magnesium ion: D20 (= D28), D24 (= D32), V26 (≠ I34), D28 (= D36)
3wy4B Crystal structure of alpha-glucosidase mutant e271q in complex with maltose (see paper)
49% identity, 68% coverage: 12:373/535 of query aligns to 4:323/421 of 3wy4B
- active site: D75 (= D105), R175 (= R205), D177 (= D207), Q237 (≠ E271)
- binding glycerol: S79 (≠ A109), N140 (≠ Q170), D147 (≠ Q177), F181 (≠ H211), Y182 (≠ S212), F183 (≠ M213), H184 (= H214), D209 (≠ S243)
4mazA The structure of mall mutant enzyme v200s from bacillus subtilus (see paper)
36% identity, 94% coverage: 12:514/535 of query aligns to 2:532/559 of 4mazA
O06994 Oligo-1,6-glucosidase 1; Dextrin 6-alpha-D-glucanohydrolase; Oligosaccharide alpha-1,6-glucosidase 1; Sucrase-isomaltase 1; Isomaltase 1; EC 3.2.1.10 from Bacillus subtilis (strain 168) (see paper)
36% identity, 94% coverage: 12:514/535 of query aligns to 4:534/561 of O06994
- D20 (= D28) binding Ca(2+)
- N22 (= N30) binding Ca(2+)
- D24 (= D32) binding Ca(2+)
- F26 (≠ I34) binding Ca(2+)
- D28 (= D36) binding Ca(2+)
7lv6B The structure of mall mutant enzyme s536r from bacillus subtilis
36% identity, 94% coverage: 12:514/535 of query aligns to 2:532/559 of 7lv6B
2ze0A Alpha-glucosidase gsj (see paper)
36% identity, 97% coverage: 10:530/535 of query aligns to 2:524/531 of 2ze0A
5wczA Crystal structure of wild-type mall from bacillus subtilis with ts analogue 1-deoxynojirimycin (see paper)
36% identity, 94% coverage: 12:514/535 of query aligns to 2:529/556 of 5wczA