SitesBLAST
Comparing WP_066609128.1 NCBI__GCF_001046645.1:WP_066609128.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
58% identity, 99% coverage: 3:482/487 of query aligns to 1:480/482 of P25526
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
58% identity, 98% coverage: 5:482/487 of query aligns to 2:479/481 of 3jz4A
- active site: N156 (= N159), K179 (= K182), E254 (= E257), C288 (= C291), E385 (= E388), E462 (= E465)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P157), W155 (= W158), K179 (= K182), A181 (≠ S184), S182 (≠ E185), A212 (≠ P215), G216 (= G219), G232 (= G235), S233 (= S236), I236 (≠ V239), C288 (= C291), K338 (= K341), E385 (= E388), F387 (= F390)
8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
56% identity, 97% coverage: 5:478/487 of query aligns to 20:493/505 of 8of1A
- binding nicotinamide-adenine-dinucleotide: I170 (= I155), A171 (≠ T156), P172 (= P157), W173 (= W158), K197 (= K182), A230 (≠ P215), F248 (= F233), G250 (= G235), S251 (= S236), V254 (= V239), M257 (≠ L242), L273 (= L258), C306 (= C291), K356 (= K341), E403 (= E388), F405 (= F390)
8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
53% identity, 99% coverage: 5:485/487 of query aligns to 2:482/482 of 8c54A
- binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I155), T153 (= T156), P154 (= P157), K179 (= K182), A212 (≠ P215), K213 (≠ T216), F230 (= F233), T231 (= T234), G232 (= G235), S233 (= S236), V236 (= V239), W239 (≠ L242), G256 (= G259)
P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
52% identity, 98% coverage: 9:485/487 of query aligns to 57:535/535 of P51649
- C93 (= C47) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
- G176 (= G130) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
- H180 (≠ P134) to Y: 83% of activity; dbSNP:rs2760118
- P182 (= P136) to L: 48% of activity; dbSNP:rs3765310
- R213 (= R167) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- C223 (= C177) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
- KPAE 228:231 (≠ KPSE 182:185) binding NAD(+)
- T233 (= T187) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
- A237 (= A191) to S: 65% of activity; dbSNP:rs62621664
- N255 (= N209) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
- G268 (= G219) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
- GSTTTG 284:289 (≠ GSTRVG 235:240) binding NAD(+)
- R334 (= R285) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- N335 (= N286) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
- C340 (= C291) modified: Disulfide link with 342, In inhibited form
- C342 (= C293) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
- N372 (≠ P322) natural variant: N -> S
- P382 (= P332) to L: in SSADHD; 2% of activity
- V406 (≠ L356) to I: in dbSNP:rs143741652
- G409 (≠ Q359) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
- S498 (≠ A448) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- G533 (≠ A483) to R: in SSADHD; <1% of activity; dbSNP:rs72552284
Sites not aligning to the query:
- 36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
52% identity, 98% coverage: 9:485/487 of query aligns to 7:485/485 of 2w8rA
2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
52% identity, 98% coverage: 9:485/487 of query aligns to 7:485/485 of 2w8qA
6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
38% identity, 96% coverage: 14:482/487 of query aligns to 3:475/477 of 6j76A
- active site: N148 (= N159), E246 (= E257), C280 (= C291), E458 (= E465)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I155), T145 (= T156), A146 (≠ P157), W147 (= W158), N148 (= N159), K171 (= K182), T173 (≠ S184), S174 (≠ E185), G204 (≠ P215), G208 (= G219), T223 (= T234), G224 (= G235), S225 (= S236), A228 (≠ V239), S231 (≠ L242), I232 (≠ L243), E246 (= E257), L247 (= L258), C280 (= C291), E381 (= E388), F383 (= F390), H447 (≠ F454)
5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
42% identity, 96% coverage: 16:482/487 of query aligns to 8:474/476 of 5x5uA
- active site: N151 (= N159), K174 (= K182), E249 (= E257), C283 (= C291), E380 (= E388), E457 (= E465)
- binding glycerol: D15 (≠ G23), A16 (= A24), A17 (≠ T25), G19 (= G27)
- binding nicotinamide-adenine-dinucleotide: P149 (= P157), P207 (= P215), A208 (≠ T216), S211 (≠ G219), G227 (= G235), S228 (= S236), V231 (= V239), R329 (≠ A337), R330 (≠ A338), E380 (= E388), F382 (= F390)
5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
42% identity, 96% coverage: 16:482/487 of query aligns to 8:474/476 of 5x5tA