SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066917916.1 NCBI__GCF_001579945.1:WP_066917916.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
43% identity, 94% coverage: 18:590/611 of query aligns to 93:656/664 of P09114

query
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P09114

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P191 (= P125) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W507) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
43% identity, 96% coverage: 16:603/611 of query aligns to 92:679/687 of P07342

query
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P07342

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R241 (= R174) binding FAD
355:376 (vs. 286:307, 55% identical) binding FAD
407:426 (vs. 330:352, 26% identical) binding FAD

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
43% identity, 94% coverage: 18:590/611 of query aligns to 96:659/667 of P09342

query
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P09342

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C161 (= C92) modified: Disulfide link with 307
P194 (= P125) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V242) modified: Disulfide link with 161

6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
43% identity, 96% coverage: 16:603/611 of query aligns to 12:599/607 of 6u9dB

query
sites
6u9dB

M

L

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K

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A

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K

G

G

P

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A

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F

L

L

L

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E

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M

E

I

V

D

D

P

K

D

K

A

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G

P

V

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Y

L

P

P

M

M

V

V

G

A

P

G

G

G

Q

S

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G

Y

L

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D

E

E

M

F

I

I

T

N

G

F

E

D

H

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E

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L

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R

active site: Y33 (= Y37), G35 (= G39), G36 (= G40), A37 (= A41), I38 (= I42), E59 (= E72), T82 (= T95), F121 (= F134), Q122 (= Q135), E123 (= E136), K171 (= K184), M274 (= M285), V301 (= V312), V417 (= V418), G443 (= G444), M445 (= M446), D470 (= D471), N497 (= N498), E499 (≠ G500), Q500 (≠ D501), M502 (= M503), V503 (= V504), W506 (= W507)
binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (= G40), V111 (= V124), P112 (= P125), F121 (= F134), K171 (= K184), D299 (= D310), R300 (= R311), M502 (= M503), W506 (= W507)
binding flavin-adenine dinucleotide: R161 (= R174), A228 (≠ G240), G229 (= G241), N232 (vs. gap), T254 (= T265), L255 (= L266), Q256 (≠ M267), L272 (= L283), M274 (= M285), G294 (= G305), R296 (= R307), D298 (= D309), R300 (= R311), V301 (= V312), E327 (≠ D330), V328 (≠ I331), N332 (≠ E335), D346 (≠ E352), A347 (= A353), M422 (= M423), G440 (= G441), G441 (≠ S442)
binding magnesium ion: D470 (= D471), N497 (= N498)
binding thiamine diphosphate: E59 (= E72), P85 (= P98), V417 (= V418), G418 (= G419), Q419 (= Q420), H420 (= H421), G443 (= G444), M445 (= M446), A471 (≠ S472), S472 (= S473), N497 (= N498), E499 (≠ G500), Q500 (≠ D501), G501 (= G502), M502 (= M503), V503 (= V504)

1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
43% identity, 96% coverage: 16:603/611 of query aligns to 8:588/596 of 1t9cA

query
sites
1t9cA

M

L

T

T

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E

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F

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A
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I
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L

L

P

P

T

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Y

Y

D

D

A

A

I

I

F

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-

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H

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D

C

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E

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E
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E

Q

Q

G

G

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A

G

G

F

H

M

M

A

A

A

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G

G

Y

Y

A

A

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R

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A

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S

G

G

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K

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P

G

G

V

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C

V

I

L

V

V

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

V

V

V

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T

P

P

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M

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A

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K

K

H

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F

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P

V

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E

K

E

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I

I

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N

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E

A

A

F

F

E

E

I

I

A

A

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S

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R
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R

P

P

G

G

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P

V

V

V

L

I

V

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D

V

L

P

P

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|
K

D

D

V

V

Q

T

N

-

W

-

Q

-

G

-

K

-

F

-

Q

A

G

A

A

I

G

L

R

R

L

N

P

P

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I

A

P

G

-

Y

-

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-

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M

-

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T

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H

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C

A

A

Q

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D

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E

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F

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M

-

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I

-

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-

L

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Y

A

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x
A

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G

V

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x
N

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x
R

A

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E

E

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A

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I

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P

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T

L
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L

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x
Q

G

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L

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F

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D

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Q

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D

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P

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|
L

G

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H
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H

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C

A

A

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A

A

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Y

L

A

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D

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A

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A
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R

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V

A

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G

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I

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F

F

A

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A

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-

A

-

E

-

G

-

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-

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I

A

I

Q

H

I

F

D
x
E

I
x
V

D

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I

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S

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E
x
N

I

I

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N

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K

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V

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Q

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H

-

W

-

H

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H

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L

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x
G

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x
D

A
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A

L

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L

I

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Y

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M

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Y

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A

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Y

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M

-

E

E

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I

I

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P

Y

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V

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I

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L

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S

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K

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A

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N

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D

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T

R

G

G

R

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H

A

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I

I

I

V

T

T

G

G

V
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V

G

G

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M

W

W

A

A

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Q

Y

H

F

W

D

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W

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N

P

P

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L

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W

F

L

I

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S

S

G
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G

S
x
G

M
x
L

G
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G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

I

I

G

G

A

A

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Q

F

V

A

A

Q

K

P

P

D

E

R

S

L

L

V

V

I

I

D

D

I

I

D

D

G

G

D
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D

S

A

S

S

I

F

R

N

M

M

N

T

L

L

G

T

E

E

L

L

E

S

T

S

V

A

T

V

T

Q

Y

A

G

G

L

T

P

P

I

V

K

K

V

I

V

L

V

I

L

L

N

N

N
|
N

C

E

G
x
E

D
x
Q

G

G

M
|
M

V
|
V

K

T

Q

Q

W
|
W

Q

Q

K

S

L

L

F

F

F

Y

K

E

G

H

R

R

L

Y

A

S

A

H

S

T

D

H

R

Q

S

L

L

-

H

-

K

N

K

P

D

D

F

F

L

I

K

K

A
x
L

A

A

E

E

A

A

D

M

G
|
G

F
x
L

P

K

Y

G

V

-

M

L

R

R

L

V

E

K

R

K

P

Q

Q

E

D

E

V

L

A

D

R

A

V

K

V

L

K

K

E

E

F

F

V

V

E

S

F

T

Q

K

G

G

P

P

A

V

F

L

L

L

E

E

V

V

M

E

I

V

D

D

P
x
K

D

K

A

V

G

P

V

V

Y

L

P

P

M

M

V

V

G

A

P

G

G

G

Q

S

P

G

Y

L

S

D

E

E

M

F

I

I

T

N

G

F

E

D

H

P

I

E

V

V

S

E

R

R

H

Q

Q

Q

I

T

E

E

V

L

R

R

active site: Y29 (= Y37), G31 (= G39), G32 (= G40), A33 (= A41), I34 (= I42), E55 (= E72), T78 (= T95), F117 (= F134), Q118 (= Q135), E119 (= E136), K167 (= K184), R227 (≠ Q249), M263 (= M285), V290 (= V312), V406 (= V418), L431 (≠ M443), G432 (= G444), M434 (= M446), D459 (= D471), N486 (= N498), E488 (≠ G500), Q489 (≠ D501), M491 (= M503), V492 (= V504), W495 (= W507), L517 (≠ A531), G522 (= G536), L523 (≠ F537), K556 (≠ P571)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G40), V107 (= V124), P108 (= P125), F117 (= F134), K167 (= K184), D288 (= D310), R289 (= R311), W495 (= W507)
binding flavin-adenine dinucleotide: R157 (= R174), G216 (= G239), A217 (≠ G240), G218 (= G241), N221 (vs. gap), T243 (= T265), L244 (= L266), Q245 (≠ M267), L261 (= L283), M263 (= M285), H264 (= H286), G283 (= G305), A284 (= A306), R285 (= R307), D287 (= D309), R289 (= R311), V290 (= V312), E316 (≠ D330), V317 (≠ I331), N321 (≠ E335), G334 (≠ P351), D335 (≠ E352), A336 (= A353), M411 (= M423), G429 (= G441), G430 (≠ S442)
binding magnesium ion: D459 (= D471), N486 (= N498), E488 (≠ G500)

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
43% identity, 96% coverage: 16:603/611 of query aligns to 8:588/596 of 1t9dA

query
sites
1t9dA

M

L

T

T

G

G

A

G

E

Q

I

I

I

F

V

N

Q

E

V

M

L

M

A

S

D

R

E

Q

G

N

V

V

D

D

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T

V

V

F

F

G

G

Y
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Y

S

P

G
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G

A
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I
|
I

L

L

P

P

T

V

Y

Y

D

D

A

A

I

I

F

-

V

-

N

H

N

N

Q

S

D

D

C

-

E

-

R

-

C

-

D

-

R

-

Q

K

M

F

S

N

L

F

I

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V

L

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P

A

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N

H

E
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E

Q

Q

G

G

A

A

G

G

F

H

M

M

A

A

A

E

G

G

Y

Y

A

A

R

R

A

A

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S

G

G

K

K

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P

G

G

V

V

C

V

I

L

V

V

T
|
T

S

S

G

G

P
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P

G

G

A

A

T

T

N

N

T

V

V

V

T

T

P

P

V

M

R

A

D

D

C

A

M

F

A

A

D

D

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I

I

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M

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I

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A

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I

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D

A

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E
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E

A

A

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D

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A

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I

I

M

S

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A

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C

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K

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E

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E

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I

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E

A

A

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E

I

I

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A

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R

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L

I

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D

V

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K

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D

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V

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N

-

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-

Q

-

G

-

K

-

F

-

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A

G

A

A

I

G

L

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R

L

N

P

P

V

I

A

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G

-

Y

-

R

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-

R

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M

-

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R

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T

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K

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T

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A

A

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E

D

F

E

F

F

T

V

M

M

-

Q

-

S

-

I

-

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-

K

-

A

-

D

-

L

L

I

G

N

A

L

A

A

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K

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P

L

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I

L

Y

Y

A

V

G
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G

G
x
A

G
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G

V

I

I

L

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x
N

H

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A

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D

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x
R

A

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Q

K

E

E

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A

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I

I

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P

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V

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T
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L
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L

M
x
Q

G

G

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L

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D

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E

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D

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P

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M

L
|
L

G

G

M
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M

H
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H

G

G

A

C

A

A

F

T

A

A

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Y

L

A

A

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D

N

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A

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D

F

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A

A

L

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A
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A

R
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R

F

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R

V
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A

T

G

G

N

N

P

I

A

S

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K

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F

A

A

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P

N

E

A

A

K

R

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R

-

A

-

E

-

G

-

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I

I

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I

Q

H

I

F

D
x
E

I
x
V

D

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I

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S

K

E
x
N

I

I

N

N

K

K

V

V

K

V

Q

Q

V

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H

-

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-

H

-

H

Q

I

I

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A

L

V

L

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x
G

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x
D

A
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A

L

T

R

T

G

N

L

L

I

G

D

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Y

M

G

M

R

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S

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A

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R

K

D

E

W

R

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S

T

E

W

W

R

F

T

A

H

Q

C

I

D

N

Q

K

L

W

R

K

T

E

Y

Y

A

P

M

Y

N

A

Y

Y

-

M

-

E

E

E

R

T

D

P

S

G

E

S

R

K

I

I

Q

K

P

P

Y

Q

H

T

V

V

I

I

E

K

I

L

N

S

K

K

L

V

-

A

-

N

-

D

T

T

R

G

G

R

E

H

A

V

I

I

I

V

T

T

G

G

V
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V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

Q

Q

Y

H

F

W

D

T

F

W

R

R

S

N

P

P

R

H

L

T

W

F

L

I

T

T

S

S

G
|
G

S
x
G

M
x
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

I

I

G

G

A

A

Q

Q

F

V

A

A

Q

K

P

P

D

E

R

S

L

L

V

V

I

I

D

D

I

I

D

D

G

G

D
|
D

S

A

S

S

I

F

R

N

M
|
M

N

T

L

L

G

T

E

E

L

L

E

S

T

S

V

A

T

V

T

Q

Y

A

G

G

L

T

P

P

I

V

K

K

V

I

V

L

V

I

L

L

N

N

N
|
N

C

E

G
x
E

D
x
Q

G

G

M
|
M

V
|
V

K

T

Q

Q

W
|
W

Q

Q

K

S

L

L

F

F

F

Y

K

E

G

H

R

R

L

Y

A

S

A

H

S

T

D

H

R

Q

S

L

L

-

H

-

K

N

K

P

D

D

F

F

L

I

K

K

A
x
L

A

A

E

E

A

A

D

M

G
|
G

F
x
L

P

K

Y

G

V

-

M

L

R

R

L

V

E

K

R

K

P

Q

Q

E

D

E

V

L

A

D

R

A

V

K

V

L

K

K

E

E

F

F

V

V

E

S

F

T

Q

K

G

G

P

P

A

V

F

L

L

L

E

E

V

V

M

E

I

V

D

D

P
x
K

D

K

A

V

G

P

V

V

Y

L

P

P

M

M

V

V

G

A

P

G

G

G

Q

S

P

G

Y

L

S

D

E

E

M

F

I

I

T

N

G

F

E

D

H

P

I

E

V

V

S

E

R

R

H

Q

Q

Q

I

T

E

E

V

L

R

R

active site: Y29 (= Y37), G31 (= G39), G32 (= G40), A33 (= A41), I34 (= I42), E55 (= E72), T78 (= T95), F117 (= F134), Q118 (= Q135), E119 (= E136), K167 (= K184), R227 (≠ Q249), M263 (= M285), V290 (= V312), V406 (= V418), L431 (≠ M443), G432 (= G444), M434 (= M446), D459 (= D471), N486 (= N498), E488 (≠ G500), Q489 (≠ D501), M491 (= M503), V492 (= V504), W495 (= W507), L517 (≠ A531), G522 (= G536), L523 (≠ F537), K556 (≠ P571)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G40), A33 (= A41), V107 (= V124), P108 (= P125), F117 (= F134), K167 (= K184), M263 (= M285), D288 (= D310), R289 (= R311), W495 (= W507)
binding flavin-adenine dinucleotide: R157 (= R174), G216 (= G239), A217 (≠ G240), G218 (= G241), N221 (vs. gap), T243 (= T265), L244 (= L266), Q245 (≠ M267), M260 (= M282), L261 (= L283), H264 (= H286), G283 (= G305), A284 (= A306), R285 (= R307), D287 (= D309), R289 (= R311), V290 (= V312), E316 (≠ D330), V317 (≠ I331), N321 (≠ E335), G334 (≠ P351), D335 (≠ E352), A336 (= A353), Q410 (= Q422), M411 (= M423), G429 (= G441), G430 (≠ S442)
binding magnesium ion: D459 (= D471), N486 (= N498), E488 (≠ G500)
binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E72), P81 (= P98), Q118 (= Q135), G432 (= G444), M434 (= M446), M464 (= M476)

1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
43% identity, 96% coverage: 16:603/611 of query aligns to 9:589/597 of 1t9aA

query
sites
1t9aA

M

L

T

T

G

G

A

G

E

Q

I

I

I

F

V

N

Q

E

V

M

L

M

A

S

D

R

E

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

Y
|
Y

S

P

G
|
G

A
|
A

I
|
I

L

L

P

P

T

V

Y

Y

D

D

A

A

I

I

F

-

V

-

N

H

N

N

Q

S

D

D

C

-

E

-

R

-

C

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active site: Y30 (= Y37), G32 (= G39), G33 (= G40), A34 (= A41), I35 (= I42), E56 (= E72), T79 (= T95), F118 (= F134), Q119 (= Q135), E120 (= E136), K168 (= K184), R228 (≠ Q249),