SitesBLAST

P62707 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; BPG-dependent PGAM; PGAM; Phosphoglyceromutase; dPGM; EC 5.4.2.11 from Escherichia coli (strain K12) (see 6 papers)
61% identity, 98% coverage: 4:249/250 of query aligns to 5:250/250 of P62707

query
sites
P62707

K

K

L

L

V

V

L

L

I

V

R
|
R

H
|
H

G
|
G

Q
x
E

S
|
S

T
x
Q

W
|
W

N
|
N

L
x
K

E

E

N

N

L

R

F

F

T
|
T

G
|
G

W

W

V

Y

D

D

V

V

D

D

L

L

T

S

D

E

Q

K

G

G

L

V

A

S

E

E

A

A

R

K

A

A

G

G

R

K

L

L

K

K

T

E

E

E

G

G

F

Y

A

S

F

F

D

D

L

F

A

A

F

Y

T

T

S

S

V

V

L

L

K

K

R

R

A

A

I

I

R

H

T

T

L

L

W

W

V

N

I

V

L

L

D

D

E

E

M

L

D

D

L

Q

M

A

W

W

L

L

P

P

V

V

E

E

R

K

S

S

W

W

R

K

L

L

N

N

E
|
E

R
|
R

H
|
H

Y
|
Y

G

G

A

A

L

L

Q

Q

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

E

K
|
K

H

Y

G

G

A

D

E

E

Q

Q

V

V

K

K

L

Q

W

W

R
|
R

S

G

Y

F

D

A

V

V

P

T

P

P

P

E

L

L

P

T

I

K

D

D

P

E

R

R

H

Y

P

P

R

G

F

H

E

D

R

P

R

R

Y

Y

A

A

S

K

L

L

P

S

P

E

Q

K

E

E

L

L

P

P

A

L

A

T

E

E

S

S

L

L

K

A

T

L

T

T

L

I

D

D

R

R

V

V

L

I

P

P

Y

Y

W

W

N

N

S

E

R

T

L

I

A

L

P

P

E

R

L

M

A

K

A

S

G

G

R

E

N

R

V

V

L

I

V

I

V

A

A

A

H
|
H

G
|
G

N
|
N

S

S

L

L

R

R

A

A

L

L

V

V

K

K

M

Y

L

L

D

D

G

N

M

M

S

S

D

E

T

E

D

E

I

I

V

L

E

E

F

L

N

N

I

I

P

P

T

T

G

G

V

V

P

P

I

L

H

V

Y

Y

E

E

L

F

D

D

D

E

A

N

L

F

K

K

P

P

V

L

S

K

R

R

R

Y

F

Y

L

L

G

G

D

N

P

A

E

D

A

E

I

I

K

A

A

A

A

K

A

A

D

A

A

A

V

V

A

A

K

N

Q

Q

T

G

E

K

T

A

K

K

10:17 (vs. 9:16, 75% identical) binding substrate
H11 (= H10) active site, Tele-phosphohistidine intermediate
K18 (≠ L17) modified: N6-acetyllysine
TG 23:24 (= TG 22:23) binding substrate
E89 (= E88) active site, Proton donor/acceptor
ERHY 89:92 (= ERHY 88:91) binding substrate
K100 (= K99) binding substrate; modified: N6-acetyllysine
K106 (= K105) modified: N6-acetyllysine
RR 116:117 (= RR 115:116) binding substrate
H184 (= H183) Transition state stabilizer
GN 185:186 (= GN 184:185) binding substrate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q3JWH7 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; BPG-dependent PGAM; PGAM; Phosphoglyceromutase; dPGM; EC 5.4.2.11 from Burkholderia pseudomallei (strain 1710b) (see paper)
64% identity, 92% coverage: 4:233/250 of query aligns to 3:232/249 of Q3JWH7

query
sites
Q3JWH7

K

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G

G

Q

E

S

S

T

T

W

W

N
|
N

L

K

E

E

N

N

L

R

F

F

T
|
T

G
|
G

W

W

V

V

D

D

V

V

D

D

L

L

T

T

D

E

Q

Q

G

G

L

N

A

R

E

E

A

A

R

R

A

Q

A

A

G

G

R

Q

L

L

K

K

T

E

E

A

G

G

F

Y

A

T

F

F

D

D

L

I

A

A

F

Y

T

T

S

S

V

V

L

L

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

H

I

V

L

Q

D

D

E

Q

M

M

D

D

L

L

M

M

W

Y

L

V

P

P

V

V

E

V

R

H

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y
|
Y

G

G

A

A

L

L

Q

S

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

Y

G

G

A

D

E

E

Q

Q

V

V

K

L

L

V

W

W

R
|
R

S

S

Y

Y

D

D

V

T

P

P

P

A

L

L

P

E

I

P

D

G

D

D

P

E

R

R

H

A

P

P

R

Y

F

A

E

D

R

P

R

R

Y

Y

A

A

S

K

L

V

P

P

P

R

Q

E

E

Q

L

L

P

P

A

L

A

T

E

E

S

C

L

L

K

K

T

D

T

T

L

V

D

A

R

R

V

V

L

L

P

P

Y

L

W

W

N

N

S

E

R

S

L

I

A

A

P

P

E

A

L

V

A

K

A

A

G

G

R

K

N

Q

V

V

L

L

V

I

V

A

A

A

H
|
H

G
|
G

N
|
N

S

S

L

L

R

R

A

A

L

L

V

I

K

K

M

Y

L

L

D

D

G

G

M

I

S

S

D

D

T

A

D

D

I

I

V

V

E

G

F

L

N

N

I

I

P

P

T

N

G

G

V

V

P

P

I

L

H

V

Y

Y

E

E

L

L

D

D

D

E

A

S

L

L

K

T

P

P

V

I

S

R

R

H

R

Y

F

Y

L

L

G

G

D

D

P

Q

E

E

R8 (= R9) binding (2R)-2,3-bisphosphoglycerate
H9 (= H10) active site, Tele-phosphohistidine intermediate; binding (2R)-2,3-bisphosphoglycerate
N15 (= N16) binding (2R)-2,3-bisphosphoglycerate
T21 (= T22) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
G22 (= G23) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R60 (= R61) binding (2R)-2,3-bisphosphoglycerate
E87 (= E88) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
Y90 (= Y91) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
K98 (= K99) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R114 (= R115) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R115 (= R116) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
H182 (= H183) binding (2R)-2,3-bisphosphoglycerate
G183 (= G184) binding (2R)-2,3-bisphosphoglycerate
N184 (= N185) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate

3gp5A Crystal structure of phosphoglyceromutase from burkholderia pseudomallei with 3-phosphoglyceric acid and vanadate (see paper)
64% identity, 92% coverage: 4:233/250 of query aligns to 3:232/248 of 3gp5A

query
sites
3gp5A

K

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G

G

Q

E

S

S

T

T

W

W

N
|
N

L

K

E

E

N

N

L

R

F

F

T

T

G

G

W

W

V

V

D

D

V

V

D

D

L

L

T

T

D

E

Q

Q

G

G

L

N

A

R

E

E

A

A

R

R

A

Q

A

A

G

G

R

Q

L

L

K

K

T

E

E

A

G

G

F

Y

A

T

F

F

D

D

L

I

A

A

F

Y

T

T

S

S

V

V

L

L

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

H

I

V

L

Q

D

D

E

Q

M

M

D

D

L

L

M

M

W

Y

L

V

P

P

V

V

E

V

R

H

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y

Y

G

G

A

A

L

L

Q

S

G

G

L

L

D

N

K

K

A

A

Q

E

T

T

V

A

H

A

K

K

H

Y

G

G

A

D

E

E

Q

Q

V

V

K

L

L

V

W

W

R

R

S

S

Y

Y

D

D

V

T

P

P

P

A

L

L

P

E

I

P

D

G

D

D

P

E

R

R

H

A

P

P

R

Y

F

A

E

D

R

P

R

R

Y

Y

A

A

S

K

L

V

P

P

P

R

Q

E

E

Q

L

L

P

P

A

L

A

T

E

E

S

C

L

L

K

K

T

D

T

T

L

V

D

A

R

R

V

V

L

L

P

P

Y

L

W

W

N

N

S

E

R

S

L

I

A

A

P

P

E

A

L

V

A

K

A

A

G

G

R

K

N

Q

V

V

L

L

V

I

V

A

A

A

H
|
H

G
|
G

N

N

S

S

L

L

R

R

A

A

L

L

V

I

K

K

M

Y

L

L

D

D

G

G

M

I

S

S

D

D

T

A

D

D

I

I

V

V

E

G

F

L

N

N

I

I

P

P

T

N

G

G

V

V

P

P

I

L

H

V

Y

Y

E

E

L

L

D

D

D

E

A

S

L

L

K

T

P

P

V

I

S

R

R

H

R

Y

F

Y

L

L

G

G

D

D

P

Q

E

E

active site: H9 (= H10), R60 (= R61), E87 (= E88), H182 (= H183)
binding vanadate ion: R8 (= R9), H9 (= H10), N15 (= N16), R60 (= R61), E87 (= E88), H182 (= H183), G183 (= G184)

3fdzA Crystal structure of phosphoglyceromutase from burkholderia pseudomallei 1710b with bound 2,3-diphosphoglyceric acid and 3- phosphoglyceric acid (see paper)
64% identity, 91% coverage: 4:231/250 of query aligns to 3:230/230 of 3fdzA

query
sites
3fdzA

K

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G

G

Q

E

S

S

T

T

W

W

N
|
N

L

K

E

E

N

N

L

R

F

F

T
|
T

G

G

W

W

V

V

D

D

V

V

D

D

L

L

T

T

D

E

Q

Q

G

G

L

N

A

R

E

E

A

A

R

R

A

Q

A

A

G

G

R

Q

L

L

K

K

T

E

E

A

G

G

F

Y

A

T

F

F

D

D

L

I

A

A

F

Y

T

T

S

S

V

V

L

L

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

H

I

V

L

Q

D

D

E

Q

M

M

D

D

L

L

M

M

W

Y

L

V

P

P

V

V

E

V

R

H

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y
|
Y

G

G

A

A

L

L

Q

S

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

Y

G

G

A

D

E

E

Q

Q

V

V

K

L

L

V

W

W

R
|
R

S

S

Y

Y

D

D

V

T

P

P

P

A

L

L

P

E

I

P

D

G

D

D

P

E

R

R

H

A

P

P

R

Y

F

A

E

D

R

P

R

R

Y

Y

A

A

S

K

L

V

P

P

P

R

Q

E

E

Q

L

L

P

P

A

L

A

T

E

E

S

C

L

L

K

K

T

D

T

T

L

V

D

A

R

R

V

V

L

L

P

P

Y

L

W

W

N

N

S

E

R

S

L

I

A

A

P

P

E

A

L

V

A

K

A

A

G

G

R

K

N

Q

V

V

L

L

V

I

V

A

A

A

H
|
H

G
|
G

N
|
N

S

S

L

L

R

R

A

A

L

L

V

I

K

K

M

Y

L

L

D

D

G

G

M

I

S

S

D

D

T

A

D

D

I

I

V

V

E

G

F

L

N

N

I

I

P

P

T

N

G

G

V

V

P

P

I

L

H

V

Y

Y

E

E

L

L

D

D

D

E

A

S

L

L

K

T

P

P

V

I

S

R

R

H

R

Y

F

Y

L

L

G

G

D

D

active site: H9 (= H10), R60 (= R61), E87 (= E88), H182 (= H183)
binding (2R)-2,3-diphosphoglyceric acid: R8 (= R9), H9 (= H10), N15 (= N16), T21 (= T22), R60 (= R61), E87 (= E88), Y90 (= Y91), K98 (= K99), R114 (= R115), R115 (= R116), H182 (= H183), G183 (= G184), N184 (= N185)

3gp3A Crystal structure of phosphoglyceromutase from burkholderia pseudomallei with 2-phosphoserine (see paper)
64% identity, 91% coverage: 4:230/250 of query aligns to 3:229/229 of 3gp3A

query
sites
3gp3A

K

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G

G

Q

E

S

S

T

T

W

W

N

N

L

K

E

E

N

N

L

R

F

F

T
|
T

G

G

W

W

V

V

D

D

V

V

D

D

L

L

T

T

D

E

Q

Q

G

G

L

N

A

R

E

E

A

A

R

R

A

Q

A

A

G

G

R

Q

L

L

K

K

T

E

E

A

G

G

F

Y

A

T

F

F

D

D

L

I

A

A

F

Y

T

T

S

S

V

V

L

L

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

H

I

V

L

Q

D

D

E

Q

M

M

D

D

L

L

M

M

W

Y

L

V

P

P

V

V

E

V

R

H

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y
|
Y

G

G

A

A

L

L

Q

S

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

Y

G

G

A

D

E

E

Q

Q

V

V

K

L

L

V

W

W

R
|
R

S

S

Y

Y

D

D

V

T

P

P

P

A

L

L

P

E

I

P

D

G

D

D

P

E

R

R

H

A

P

P

R

Y

F

A

E

D

R

P

R

R

Y

Y

A

A

S

K

L

V

P

P

P

R

Q

E

E

Q

L

L

P

P

A

L

A

T

E

E

S

C

L

L

K

K

T

D

T

T

L

V

D

A

R

R

V

V

L

L

P

P

Y

L

W

W

N

N

S

E

R

S

L

I

A

A

P

P

E

A

L

V

A

K

A

A

G

G

R

K

N

Q

V

V

L

L

V

I

V

A

A

A

H
|
H

G
|
G

N
|
N

S

S

L

L

R

R

A

A

L

L

V

I

K

K

M

Y

L

L

D

D

G

G

M

I

S

S

D

D

T

A

D

D

I

I

V

V

E

G

F

L

N

N

I

I

P

P

T

N

G

G

V

V

P

P

I

L

H

V

Y

Y

E

E

L

L

D

D

D

E

A

S

L

L

K

T

P

P

V

I

S

R

R

H

R

Y

F

Y

L

L

G

G

active site: H9 (= H10), R60 (= R61), E87 (= E88), H182 (= H183)
binding phosphite ion: R8 (= R9), H9 (= H10), R60 (= R61), E87 (= E88), H182 (= H183), G183 (= G184)
binding phosphoserine: T21 (= T22), E87 (= E88), Y90 (= Y91), K98 (= K99), R114 (= R115), R115 (= R116), N184 (= N185)

P18669 Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B; EC 5.4.2.11; EC 5.4.2.4 from Homo sapiens (Human) (see 4 papers)
57% identity, 99% coverage: 4:250/250 of query aligns to 5:254/254 of P18669

query
sites
P18669

K

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G
|
G

Q
x
E

S
|
S

T
x
A

W
|
W

N
|
N

L

L

E

E

N

N

L

R

F

F

T
x
S

G
|
G

W

W

V
x
Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H
|
H

Y
|
Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W

W

R
|
R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

A

K

A

A

A

M

D

E

A

A

V

V

A

A

K

A

Q

Q

T

G

E
x
K

T

A

K
|
K

10:17 (vs. 9:16, 75% identical) binding substrate
H11 (= H10) active site, Tele-phosphohistidine intermediate
SG 23:24 (≠ TG 22:23) binding substrate
Y26 (≠ V25) modified: Phosphotyrosine
ERHY 89:92 (= ERHY 88:91) binding substrate
K100 (= K99) binding substrate
RR 116:117 (= RR 115:116) binding substrate
K251 (≠ E247) modified: N6-acetyllysine; alternate
K253 (= K249) modified: N6-acetyllysine
K254 (= K250) modified: N6-acetyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7xb8B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
58% identity, 97% coverage: 4:245/250 of query aligns to 3:247/249 of 7xb8B

query
sites
7xb8B

K

K

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N

N

L

R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y
|
Y

G

G

A

G

L
|
L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V
|
V

K

K

L

I

W
|
W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N
|
N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

A

K

A

A

A

M

D

E

A

A

V

V

A

A

K

A

Q

Q

binding 4-(cyclopenten-1-yl)-2-[(3-sulfophenyl)sulfonylamino]benzoic acid: F20 (= F21), R88 (= R89), Y90 (= Y91), L93 (= L94), K98 (= K99), V110 (= V111), W113 (= W114), R114 (= R115), N186 (= N185)

7xb7B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
58% identity, 96% coverage: 4:243/250 of query aligns to 3:245/246 of 7xb7B

query
sites
7xb7B

K

K

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N

N

L

R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y
|
Y

G

G

A

G

L
|
L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V
|
V

K

K

L

I

W

W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N
|
N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

A

K

A

A

A

M

D

E

A

A

V

V

A

A

binding 3-[[5-(cyclopenten-1-yl)-2-(methylcarbamoyl)phenyl]sulfamoyl]benzenesulfonic acid: F20 (= F21), R88 (= R89), Y90 (= Y91), L93 (= L94), K98 (= K99), V110 (= V111), R114 (= R115), N186 (= N185)

1yfkA Crystal structure of human b type phosphoglycerate mutase (see paper)
57% identity, 95% coverage: 4:241/250 of query aligns to 3:243/243 of 1yfkA

query
sites
1yfkA

K

K

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N

N

L

R

F

F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V
|
V

E

V

R
|
R

S

T

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y

Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K

K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W

W

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

A

K

A

A

A

M

D

E

A

A

active site: H9 (= H10), R60 (= R61), E87 (= E88), H184 (= H183)
binding chloride ion: V79 (= V80), R81 (= R82)

8itdC Phosphoglycerate mutase 1 complexed with a compound
58% identity, 94% coverage: 4:238/250 of query aligns to 4:241/242 of 8itdC

query
sites
8itdC

K

K

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N

N

L

R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V
x
A

H

A

K

K

H

H

G

G

A
x
E

E

A

Q

Q

V
|
V

K

K

L

I

W
|
W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

A

K

A

A

binding 4-(4-chloranyl-2-oxidanyl-phenyl)-2-[(4-phenylphenyl)sulfonylamino]benzoic acid: F21 (= F21), R89 (= R89), K99 (= K99), A103 (≠ V103), E108 (≠ A108), V111 (= V111), W114 (= W114), R115 (= R115)

8it8C Phosphoglycerate mutase 1 complexed with a compound
58% identity, 93% coverage: 4:236/250 of query aligns to 4:239/240 of 8it8C

query
sites
8it8C

K

K

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

T

A

W

W

N
|
N

L

L

E

E

N
|
N

L
x
R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W

W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

binding 4-(4-chlorophenyl)-2-[(4-phenylphenyl)sulfonylamino]benzoic acid: N16 (= N16), N19 (= N19), R20 (≠ L20), F21 (= F21), R89 (= R89), K99 (= K99), R115 (= R115)

5zs8C Acetylation of lysine 100 of phosphoglycerate mutase 1 complexed with kh_ol
58% identity, 93% coverage: 4:236/250 of query aligns to 4:239/240 of 5zs8C

query
sites
5zs8C

K

K

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N
|
N

L

R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R

R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W

W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

binding N-(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)-2-hydroxybenzene-1-sulfonamide: N19 (= N19), F21 (= F21), R89 (= R89), K99 (= K99), R115 (= R115)

5y65C Phosphoglycerate mutase 1 complexed with a small molecule inhibitor kh2
58% identity, 93% coverage: 4:236/250 of query aligns to 3:238/239 of 5y65C

query
sites
5y65C

K

K

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N
|
N

L
x
R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H

H

Y

Y

G

G

A

G

L

L

Q

T

G

G

L

L

D

N

K
|
K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W
|
W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D

K

A

N

L

L

K

K

P

P

V

I

S

K

-

P

R

M

R

Q

F

F

L

L

G

G

D

D

P

E

E

E

A

T

I

V

K

R

active site: H9 (= H10), R60 (= R61), E87 (= E88), H184 (= H183)
binding ~{N}-[3,4-bis(oxidanyl)-9,10-bis(oxidanylidene)anthracen-2-yl]-4-(dimethylamino)benzenesulfonamide: N18 (= N19), R19 (≠ L20), F20 (= F21), R88 (= R89), K98 (= K99), W113 (= W114), R114 (= R115)

P07738 Bisphosphoglycerate mutase; BPGM; 2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase; 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM; EC 5.4.2.4; EC 5.4.2.11 from Homo sapiens (Human) (see 5 papers)
53% identity, 99% coverage: 4:250/250 of query aligns to 5:256/259 of P07738

query
sites
P07738

K
|
K

L

L

V

I

L

M

I

L

R
|
R

H
|
H

G
|
G

Q
x
E

S
x
G

T
x
A

W
|
W

N
|
N

L
x
K

E

E

N

N

L

R

F

F

T
x
C

G
x
S

W

W

V

V

D

D

V

Q

D
x
K

L

L

T

N

D

S

Q

E

G

G

L

M

A

E

E

E

A

A

R

R

A

N

A

C

G

G

R
x
K

L

Q

L

L

K
|
K

T

A

E

L

G

N

F

F

A

E

F

F

D

D

L

L

A

V

F

F

T

T

S

S

V

V

L

L

K

N

R
|
R

A

S

I

I

R

H

T

T

L

A

W

W

V

L

I

I

L

L

D

E

E

E

M

L

D

G

L

Q

M

E

W

W

L

V

P

P

V

V

E

E

R

S

S

S

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H
|
H

Y
|
Y

G

G

A

A

L

L

Q

I

G

G

L

L

D

N

K
x
R

A

E

Q

Q

T

M

V

A

H

L

K

N

H

H

G

G

A

E

E

E

Q

Q

V

V

K

R

L

L

W

W

R
|
R

S

S

Y

Y

D

N

V

V

P

T

P

P

L

I

P

E

I

E

D

S

D

H

P

P

R

Y

H

Y

P

Q

R

E

F

I

-

Y

-

N

E

D

R

R

Y

Y

-
x
K

-

V

A

C

S

D

L

V

P

P

P

L

Q

D

E

Q

L

L

P

P

A

R

A

S

E

E

S

S

L

L

K
|
K

T

D

T

V

L

L

D

E

R

R

V

L

L

L

P

P

Y

Y

W

W

N

N

S

E

R

R

L

I

A

A

P

P

E

E

L

V

A

L

A

R

G

G

R
x
K

N

T

V

I

L

L

V

I

V

S

A

A

H
|
H

G
|
G

N
|
N

S

S

L

S

R

R

A

A

L

L

V

L

K
|
K

M

H

L

L

D

E

G

G

M

I

S

S

D

D

T

E

D

D

I

I

V

I

E

N

F

I

N

T

I

L

P

P

T

T

G

G

V

V

P

P

I

I

H

L

Y

L

E

E

L

L

D

D

D

E

A

N

L

L

K

R

P

A

V

V

S

G

-

P

R

H

R

Q

F

F

L

L

G

G

D

D

P

Q

E

E

A

A

I

I

K

Q

A

A

A

I

D
x
K

A
x
K

V

V

A

E

K

D

Q

Q

T

G

E
x
K

T

V

K

K

K

Q

K5 (= K4) modified: carbohydrate, N-linked (Glc) (glycation) lysine; in vitro
10:17 (vs. 9:16, 63% identical) binding substrate
H11 (= H10) active site, Tele-phosphohistidine intermediate
K18 (≠ L17) modified: carbohydrate, N-linked (Glc) (glycation) lysine; in vitro
CS 23:24 (≠ TG 22:23) binding substrate
K29 (≠ D28) Not glycated
K43 (≠ R42) modified: carbohydrate, N-linked (Glc) (glycation) lysine; in vitro
K46 (= K45) Not glycated
R62 (= R61) binding substrate
E89 (= E88) active site, Proton donor/acceptor
ERHY 89:92 (= ERHY 88:91) binding substrate
R90 (= R89) to C: in ECYT8; mutation identified at protein level; marked decrease in synthase and mutase activities; no effect on phosphatase activity; dbSNP:rs121964925
R100 (≠ K99) binding substrate
RR 116:117 (= RR 115:116) binding substrate
K143 (vs. gap) Not glycated
K159 (= K154) modified: carbohydrate, N-linked (Glc) (glycation) lysine
K181 (≠ R176) Not glycated
H188 (= H183) Transition state stabilizer
GN 189:190 (= GN 184:185) binding substrate
K197 (= K192) modified: carbohydrate, N-linked (Glc) (glycation) lysine; in vitro
K246 (≠ D240) Not glycated
K247 (≠ A241) Not glycated
K253 (≠ E247) Not glycated

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
3 modified: carbohydrate, N-linked (Glc) (glycation) lysine; in vitro
258 Not glycated
259 Not glycated

2f90A Crystal structure of bisphosphoglycerate mutase in complex with 3- phosphoglycerate and alf4- (see paper)
53% identity, 99% coverage: 4:250/250 of query aligns to 3:254/254 of 2f90A

query
sites
2f90A

K

K

L

L

V

I

L

M

I

L

R
|
R

H
|
H

G

G

Q

E

S

G

T

A

W

W

N

N

L

K

E

E

N

N

L

R

F

F

T

C

G

S

W

W

V

V

D

D

V

Q

D

K

L

L

T

N

D

S

Q

E

G

G

L

M

A

E

E

E

A

A

R

R

A

N

A

C

G

G

R

K

L

Q

L

L

K

K

T

A

E

L

G

N

F

F

A

E

F

F

D

D

L

L

A

V

F

F

T

T

S

S

V

V

L

L

K

N

R
|
R

A

S

I

I

R

H

T

T

L

A

W

W

V

L

I

I

L

L

D

E

E

E

M

L

D

G

L

Q

M

E

W

W

L

V

P

P

V

V

E

E

R

S

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y

Y

G

G

A

A

L

L

Q

I

G

G

L

L

D

N

K

R

A

E

Q

Q

T

M

V

A

H

L

K

N

H

H

G

G

A

E

E

E

Q

Q

V

V

K

R

L

L

W

W

R

R

S

S

Y

Y

D

N

V

V

P

T

P

P

L

I

P

E

I

E

D

S

D

H

P

P

R

Y

H

Y

P

Q

R

E

F

I

-

Y

-

N

E

D

R

R

Y

Y

-

K

-

V

A

C

S

D

L

V

P

P

P

L

Q

D

E

Q

L

L

P

P

A

R

A

S

E

E

S

S

L

L

K

K

T

D

T

V

L

L

D

E

R

R

V

L

L

L

P

P

Y

Y

W

W

N

N

S

E

R

R

L

I

A

A

P

P

E

E

L

V

A

L

A

R

G

G

R

K

N

T

V

I

L

L

V

I

V

S

A

A

H
|
H

G
|
G

N

N

S

S

L

S

R

R

A

A

L

L

V

L

K

K

M

H

L

L

D

E

G

G

M

I

S

S

D

D

T

E

D

D

I

I

V

I

E

N

F

I

N

T

I

L

P

P

T

T

G

G

V

V

P

P

I

I

H

L

Y

L

E

E

L

L

D

D

D

E

A

N

L

L

K

R

P

A

V

V

S

G

-

P

R

H

R

Q

F

F

L

L

G

G

D

D

P

Q

E

E

A

A

I

I

K

Q

A

A

A

I

D

K

A

K

V

V

A

E

K

D

Q

Q

T

G

E

K

T

V

K

K

K

Q

active site: H9 (= H10), R60 (= R61), E87 (= E88), H186 (= H183)
binding tetrafluoroaluminate ion: R8 (= R9), H9 (= H10), R60 (= R61), E87 (= E88), H186 (= H183), G187 (= G184)

2h4zA Human bisphosphoglycerate mutase complexed with 2,3- bisphosphoglycerate (see paper)
53% identity, 99% coverage: 4:250/250 of query aligns to 4:255/255 of 2h4zA

query
sites
2h4zA

K

K

L

L

V

I

L

M

I

L

R
|
R

H
|
H

G

G

Q

E

S

G

T

A

W

W

N

N

L

K

E

E

N

N

L

R

F

F

T
x
C

G
x
S

W

W

V

V

D

D

V

Q

D

K

L

L

T

N

D

S

Q

E

G

G

L

M

A

E

E

E

A

A

R

R

A

N

A

C

G

G

R

K

L

Q

L

L

K

K

T

A

E

L

G

N

F

F

A

E

F

F

D

D

L

L

A

V

F

F

T

T

S

S

V

V

L

L

K

N

R
|
R

A

S

I

I

R

H

T

T

L

A

W

W

V

L

I

I

L

L

D

E

E

E

M

L

D

G

L

Q

M

E

W

W

L

V

P

P

V

V

E

E

R

S

S

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y
|
Y

G

G

A

A

L

L

Q

I

G

G

L

L

D

N

K
x
R

A

E

Q

Q

T

M

V

A

H

L

K

N

H

H

G

G

A

E

E

E

Q

Q

V

V

K

R

L

L

W

W

R
|
R

S

S

Y

Y

D

N

V

V

P

T

P

P

L

I

P

E

I

E

D

S

D

H

P

P

R

Y

H

Y

P

Q

R

E

F

I

-

Y

-

N

E

D

R

R

Y

Y

-

K

-

V

A

C

S

D

L

V

P

P

P

L

Q

D

E

Q

L

L

P

P

A

R

A

S

E

E

S

S

L

L

K

K

T

D

T

V

L

L

D

E

R

R

V

L

L

L

P

P

Y

Y

W

W

N

N

S

E

R

R

L

I

A

A

P

P

E

E

L

V

A

L

A

R

G

G

R

K

N

T

V

I

L

L

V

I

V

S

A

A

H
|
H

G

G

N
|
N

S

S

L

S

R

R

A

A

L

L

V

L

K

K

M

H

L

L

D

E

G

G

M

I

S

S

D

D

T

E

D

D

I

I

V

I

E

N

F

I

N

T

I

L

P

P

T

T

G

G

V

V

P

P

I

I

H

L

Y

L

E

E

L

L

D

D

D

E

A

N

L

L

K

R

P

A

V

V

S

G

-

P

R

H

R

Q

F

F

L

L

G

G

D

D

P

Q

E

E

A

A

I

I

K

Q

A

A

A

I

D

K

A

K

V

V

A

E

K

D

Q

Q

T

G

E

K

T

V

K

K

K

Q

active site: H10 (= H10), R61 (= R61), E88 (= E88), H187 (= H183)
binding (2R)-2,3-diphosphoglyceric acid: R9 (= R9), H10 (= H10), C22 (≠ T22), S23 (≠ G23), R61 (= R61), E88 (= E88), Y91 (= Y91), R99 (≠ K99), R115 (= R115), R116 (= R116), H187 (= H183), N189 (= N185)

5y2uB X-ray structure of phosphoglycerate mutase 1(pgam1) complexed with a small molecule
58% identity, 93% coverage: 4:235/250 of query aligns to 3:237/237 of 5y2uB

query
sites
5y2uB

K

K

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

T

A

W

W

N

N

L

L

E

E

N

N

L

R

F
|
F

T

S

G

G

W

W

V

Y

D

D

V

A

D

D

L

L

T

S

D

P

Q

A

G

G

L

H

A

E

E

E

A

A

R

K

A

R

A

G

G

G

R

Q

L

A

L

L

K

R

T

D

E

A

G

G

F

Y

A

E

F

F

D

D

L

I

A

C

F

F

T

T

S

S

V

V

L

Q

K

K

R
|
R

A

A

I

I

R

R

T

T

L

L

W

W

V

T

I

V

L

L

D

D

E

A

M

I

D

D

L

Q

M

M

W

W

L

L

P

P

V

V

E

V

R

R

S

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H

H

Y

Y

G

G

A

G

L
|
L

Q

T

G

G

L

L

D

N

K

K

A

A

Q

E

T

T

V

A

H

A

K

K

H

H

G

G

A

E

E

A

Q

Q

V

V

K

K

L

I

W
|
W

R
|
R

R

R

S

S

Y

Y

D

D

V

V

P

P

L

M

P

E

I

P

D

D

D

H

P

P

R

F

H

Y

P

S

R

N

F

I

-

S

-

K

E

D

R

R

Y

Y

A

A

S

D

L

L

P

T

P

E

Q

D

E

Q

L

L

P

P

A

S

A

C

E

E

S

S

L

L

K

K

T

D

T

T

L

I

D

A

R

R

V

A

L

L

P

P

Y

F

W

W

N

N

S

E

R

E

L

I

A

V

P

P

E

Q

L

I

A

K

A

E

G

G

R

K

N

R

V

V

L

L

V

I

V

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

M

H

L

L

D

E

G

G

M

L

S

S

D

E

T

E

D

A

I

I

V

M

E

E

F

L

N

N

I

L

P

P

T

T

G

G

V

I

P

P

I

I

H

V

Y

Y

E

E

L

L

D

D

D
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