SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066920221.1 NCBI__GCF_001579945.1:WP_066920221.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
57% identity, 95% coverage: 6:309/319 of query aligns to 2:304/304 of 1iyeA

query
sites
1iyeA

A

A

K

D

H

Y

I

I

W

W

Y

F

N

N

G

G

K

E

L

M

V

V

P

R

W

W

E

E

K

D

A

A

T

K

V

V

H

H

V

V

L

M

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

C

Y

Y

A

D

T

S

A

H

Q

K

G

G

P

P

V

V

I

V

F

F

R

R

L

H

R

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

R

K

I

I

Y

Y

R

R

I

F

D

P

I

V

P

S

Y

Q

T

S

P

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

K

R

E

D

I

V

I

I

V

R

V

K

N

N

G

N

L

L

D

-

K

T

G

S

A

A

Y
|
Y

M

I

R

R

P

P

F

L

A

I

F

F

R

V

S

G

Y

D

G

V

E

G

I

M

G

G

V

V

A

N

P

P

K

P

T

A

P

G

P

Y

P

S

I

T

D

D

T

V

V

I

V

I

A

A

A

A

F

F

E

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

A

A

D

E

G

A

L

L

E

E

N

Q

G

G

I

I

D

D

V

A

C

M

V

V

S

S

S

S

W

W

Q

N

R

R

L

A

A

A

A

P

N

N

T

T

I

I

P

P

A

T

L

A

G

A

K
|
K

A
|
A

A

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

S

G

M

S

E

E

A

A

K

R

R

R

L

H

G

G

F

Y

A

Q

E

E

G

G

I

I

G

A

L

L

G

D

I

V

D

N

G

G

S

Y

V

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N
|
N

L

L

F

F

V

E

V

V

R

K

D

D

N

G

V

V

I

L

Y

F

T

T

P

P

G

P

L

F

A

T

N

S

A

S

I

A

L
|
L

Q

P

G
|
G

I
|
I

T
|
T

R

R

D

D

T

A

V

I

I

I

T

K

L

L

A

A

R

K

I

E

H

L

G

G

F

I

E

E

V

V

R

R

E

E

Q

Q

A

V

L

L

P

S

R

R

E

E

F

S

L

L

Y

Y

L

L

A

A

D

D

E

E

I

V

F

F

L

M

T

S

G
|
G

S
x
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

I

I

E

Q

V

V

G

G

S

E

G

G

K

R

R

C

G

G

P

P

V

V

T

T

E

K

A

R

L

I

Q

Q

S

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

K

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

E

D

R

Q

C

V

D

N

active site: F33 (= F37), G35 (= G39), K156 (= K161), A157 (= A162), E190 (= E195), L214 (= L219)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y97), Y126 (= Y131), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (≠ S259), A255 (= A260)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
57% identity, 95% coverage: 6:309/319 of query aligns to 2:304/304 of 1iydA

query
sites
1iydA

A

A

K

D

H

Y

I

I

W

W

Y

F

N

N

G

G

K

E

L

M

V

V

P

R

W

W

E

E

K

D

A

A

T

K

V

V

H

H

V

V

L

M

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

C

Y

Y

A

D

T

S

A

H

Q

K

G

G

P

P

V

V

I

V

F

F

R

R

L

H

R

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

R

K

I

I

Y

Y

R

R

I

F

D

P

I

V

P

S

Y

Q

T

S

P

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

K

R

E

D

I

V

I

I

V

R

V

K

N

N

G

N

L

L

D

-

K

T

G

S

A

A

Y
|
Y

M

I

R

R

P

P

F

L

A

I

F

F

R

V

S

G

Y

D

G

V

E

G

I

M

G

G

V

V

A

N

P

P

K

P

T

A

P

G

P

Y

P

S

I

T

D

D

T

V

V

I

V

I

A

A

A

A

F

F

E

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

A

A

D

E

G

A

L

L

E

E

N

Q

G

G

I

I

D

D

V

A

C

M

V

V

S

S

S

S

W

W

Q

N

R

R

L

A

A

A

A

P

N

N

T

T

I

I

P

P

A

T

L

A

G

A

K
|
K

A
|
A

A

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

S

G

M

S

E

E

A

A

K

R

R

R

L

H

G

G

F

Y

A

Q

E

E

G

G

I

I

G

A

L

L

G

D

I

V

D

N

G

G

S

Y

V

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

V

E

V

V

R

K

D

D

N

G

V

V

I

L

Y

F

T

T

P

P

G

P

L

F

A

T

N

S

A

S

I

A

L
|
L

Q

P

G
|
G

I
|
I

T
|
T

R

R

D

D

T

A

V

I

I

I

T

K

L

L

A

A

R

K

I

E

H

L

G

G

F

I

E

E

V

V

R

R

E

E

Q

Q

A

V

L

L

P

S

R

R

E

E

F

S

L

L

Y

Y

L

L

A

A

D

D

E

E

I

V

F

F

L

M

T

S

G

G

S
x
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

I

I

E

Q

V

V

G

G

S

E

G

G

K

R

R

C

G

G

P

P

V

V

T

T

E

K

A

R

L

I

Q

Q

S

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

K

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

E

D

R

Q

C

V

D

N

active site: F33 (= F37), G35 (= G39), K156 (= K161), A157 (= A162), E190 (= E195), L214 (= L219)
binding glutaric acid: Y92 (= Y97), Y126 (= Y131), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S259)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
57% identity, 95% coverage: 6:309/319 of query aligns to 2:304/304 of 1i1mA

query
sites
1i1mA

A

A

K

D

H

Y

I

I

W

W

Y

F

N

N

G

G

K

E

L

M

V

V

P

R

W

W

E

E

K

D

A

A

T

K

V

V

H

H

V

V

L

M

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F

F

E

E

G

G

V

I

R

R

C

C

Y

Y

A

D

T

S

A

H

Q

K

G

G

P

P

V

V

I

V

F

F

R

R

L

H

R

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

R

K

I

I

Y

Y

R

R

I

F

D

P

I

V

P

S

Y

Q

T

S

P

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

K

R

E

D

I

V

I

I

V

R

V

K

N

N

G

N

L

L

D

-

K

T

G

S

A

A

Y
|
Y

M

I

R

R

P

P

F

L

A

I

F

F

R

V

S

G

Y

D

G

V

E

G

I

M

G

G

V

V

A

N

P

P

K

P

T

A

P

G

P

Y

P

S

I

T

D

D

T

V

V

I

V

I

A

A

A

A

F

F

E

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

A

D

E

G

A

L

L

E

E

N

Q

G

G

I

I

D

D

V

A

C

M

V

V

S

S

S

S

W

W

Q

N

R

R

L

A

A

A

A

P

N

N

T

T

I

I

P

P

A

T

L

A

G

A

K
|
K

A

A

A

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

S

G

M

S

E

E

A

A

K

R

R

R

L

H

G

G

F

Y

A

Q

E

E

G

G

I

I

G

A

L

L

G

D

I

V

D

N

G

G

S

Y

V

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

V

E

V

V

R

K

D

D

N

G

V

V

I

L

Y

F

T

T

P

P

G

P

L

F

A

T

N

S

A

S

I

A

L
|
L

Q

P

G
|
G

I
|
I

T
|
T

R

R

D

D

T

A

V

I

I

I

T

K

L

L

A

A

R

K

I

E

H

L

G

G

F

I

E

E

V

V

R

R

E

E

Q

Q

A

V

L

L

P

S

R

R

E

E

F

S

L

L

Y

Y

L

L

A

A

D

D

E

E

I

V

F

F

L

M

T

S

G
|
G

S
x
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

I

I

E

Q

V

V

G

G

S

E

G

G

K

R

R

C

G

G

P

P

V

V

T

T

E

K

A

R

L

I

Q

Q

S

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

K

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

E

D

R

Q

C

V

D

N

active site: K156 (= K161)
binding 4-methyl valeric acid: Y92 (= Y97), K156 (= K161), T254 (≠ S259), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (≠ S259)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
57% identity, 95% coverage: 6:309/319 of query aligns to 2:304/304 of 1i1lA

query
sites
1i1lA

A

A

K

D

H

Y

I

I

W

W

Y

F

N

N

G

G

K

E

L

M

V

V

P

R

W

W

E

E

K

D

A

A

T

K

V

V

H

H

V

V

L

M

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F

F

E

E

G

G

V

I

R

R

C

C

Y

Y

A

D

T

S

A

H

Q

K

G

G

P

P

V

V

I

V

F

F

R

R

L

H

R

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

R

K

I

I

Y

Y

R

R

I

F

D

P

I

V

P

S

Y

Q

T

S

P

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

K

R

E

D

I

V

I

I

V

R

V

K

N

N

G

N

L

L

D

-

K

T

G

S

A

A

Y
|
Y

M

I

R

R

P

P

F

L

A

I

F

F

R

V

S

G

Y

D

G

V

E

G

I

M

G

G

V

V

A

N

P

P

K

P

T

A

P

G

P

Y

P

S

I

T

D

D

T

V

V

I

V

I

A

A

A

A

F

F

E

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

A

D

E

G

A

L

L

E

E

N

Q

G

G

I

I

D

D

V

A

C

M

V

V

S

S

S

S

W

W

Q

N

R

R

L

A

A

A

A

P

N

N

T

T

I

I

P

P

A

T

L

A

G

A

K
|
K

A

A

A

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

S

G

M

S

E

E

A

A

K

R

R

R

L

H

G

G

F

Y

A

Q

E

E

G

G

I

I

G

A

L

L

G

D

I

V

D

N

G

G

S

Y

V

I

S

S

E
|
E

G

G

A

A

G
|
G

E

E

N

N

L

L

F

F

V

E

V

V

R

K

D

D

N

G

V

V

I

L

Y

F

T

T

P

P

G

P

L

F

A

T

N

S

A

S

I

A

L

L

Q

P

G
|
G

I
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I

T
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T

R

R

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D

T

A

V

I

I

I

T

K

L

L

A

A

R

K

I

E

H

L

G

G

F

I

E

E

V

V

R

R

E

E

Q

Q

A

V

L

L

P

S

R

R

E

E

F

S

L

L

Y

Y

L

L

A

A

D

D

E

E

I

V

F

F

L

M

T

S

G

G

S
x
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

I

I

E

Q

V

V

G

G

S

E

G

G

K

R

R

C

G

G

P

P

V

V

T

T

E

K

A

R

L

I

Q

Q

S

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

K

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

E

D

R

Q

C

V

D

N

active site: K156 (= K161)
binding 2-methylleucine: Y92 (= Y97), K156 (= K161), T254 (≠ S259), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S259)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
46% identity, 93% coverage: 9:305/319 of query aligns to 7:301/305 of 2ej0B

query
sites
2ej0B

I

I

W

W

Y

M

N

N

G

G

K

A

L

F

V

V

P

P

W

Q

E

E

K

E

A

A

T

K

V

T

H

S

V

V

L

L

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

E

T

T

A

A

Q

K

G

G

P

P

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

R

K

I

V

Y

L

R

R

I

M

D

E

I

I

P

P

Y

F

T

A

P

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

K

K

E

E

I

V

I

V

V

R

V

R

N

N

G

G

L

Y

D

-

K

R

G

S

A

C

Y

Y

M

I

R

R

P

P

F

L

A

A

F

W

R

M

S

G

Y

A

G

K

E

A

I

L

G

G

V

V

A

N

P

P

K

L

T

P

P

N

P

N

P

P

I

A

D

E

T

V

V

M

V

V

A

A

A

A

F

W

E

E

W

W

G

G

A

A

Y

Y

L

L

G

G

A

E

D

E

G

A

L

V

E

R

N

K

G

G

I

A

D

R

V

L

C

I

V

T

S

S

S

S

W

W

Q

A

R

R

L

F

A

P

A

A

N

N

T

V

I

M

P

P

A

G

L

K

G

A

K
|
K

A

V

A

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

S

K

M

M

E

E

A

A

K

V

R

A

L

A

G

G

F

A

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

E

D

E

G

G

S

Y

V

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

V

F

V

V

R

R

D

D

N

G

V

V

I

I

Y

Y

T

-

P

-

G

A

L

L

A

E

N

H

A

S

I

V

-

N

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

D

T

S

V

V

I

I

T

R

L

I

A

A

R

K

I

D

H

L

G

G

F

Y

E

E

V

V

R

Q

E

V

Q

V

A

R

L

A

P

T

R

R

E

D

F

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

I

V

F

F

L

M

T

T

G
|
G

S
x
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

I

R

E

P

V

I

G

G

S

K

G

G

K

T

R

A

G

G

P

P

V

V

T

A

E

L

A

R

L

L

Q

R

S

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

K

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K158 (= K161), E192 (= E195), L215 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y166), E192 (= E195), G195 (= G198), E196 (= E199), L215 (= L219), G217 (= G221), I218 (= I222), T219 (= T223), G254 (= G258), T255 (≠ S259)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
47% identity, 94% coverage: 9:307/319 of query aligns to 9:305/306 of 4whxA

query
sites
4whxA

I

I

W

W

Y

M

N

D

G

G

K

K

L

L

V

I

P

E

W

W

E

R

K

D

A

A

T

K

V

I

H

H

V

V

L

L

A

T

H

H

A

T

L

L

H

H

Y

Y

G

G

S

M

T

G

V

V

F
|
F

E

E

G

G

V

V

R

R

C

A

Y

Y

A

K

T

T

A

A

Q

D

G

G

-

G

P

T

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

T

R

K

R

R

L

L

F

L

D

N

S

S

A

A

R

K

I

I

Y

F

R

Q

I

M

D

D

I

V

P

P

Y

F

T

D

P

Q

D

E

E

T

I

L

N

E

A

A

A

A

C

Q

K

R

E

D

I

V

I

V

V

R

V

E

N

N

G

K

L

L

D

E

K

S

G

-

A

C

Y
|
Y

M

L

R

R

P

P

F

I

A

I

F

W

R

I

S

G

Y

S

G

E

E

K

I

L

G

G

V

V

A

S

P

A

K

K

T

-

P

G

P

N

P

T

I

I

D

H

T

V

V

A

V

I

A

A

A

A

F

W

E

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

E

D

E

G

G

L

L

E

A

N

K

G

G

I

I

D

R

V

V

C

K

V

T

S

S

S

S

W

F

Q

T

R

R

L

H

A

H

A

V

N

N

T

V

I

S

P

M

A

V

L

R

G

A

K
|
K

A

A

A

S

G

G

N

W

Y

Y

L

V

S

N

S

S

Q

I

L

L

I

A

S

N

M

Q

E

E

A

A

K

T

R

A

L

D

G

G

F

Y

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

V

D

D

G

G

S

Y

V

V

S

S

E
|
E

G

G

A

S

G

G

E

E

N

N

L

F

F

F

V

L

V

V

R

N

D

R

N

G

V

K

I

L

Y

Y

T

T

P

P

G

D

L

L

A

A

N

S

A

C

I

-

L
|
L

Q

D

G

G

I

I

T

T

R

R

D

D

T

T

V

V

I

I

T

T

L

L

A

A

R

K

I

E

H

A

G

G

F

I

E

E

V

V

R

I

E

E

Q

K

A

R

L

I

P

T

R

R

E

D

F

E

L

V

Y

Y

L

T

A

A

D

D

E

E

I

A

F

F

L

F

T

T

G

G

S

T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

I

R

R

S

E

V

L

D

D

R

N

I

R

E

T

V

I

G

G

S

G

G

G

K

A

R

R

G

G

P

P

V

I

T

T

E

E

A

K

L

L

Q

Q

S

S

A

A

F

F

F

F

G

D

L

V

F

V

D

N

G

G

K

K

T

S

E

A

D

K

K

H

W

A

G

D

W

W

L

L

E

T

R

K

active site: F37 (= F37), K160 (= K161), E194 (= E195), L217 (= L219)
binding alanine: Y97 (= Y97), K160 (= K161), A258 (= A260)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
45% identity, 93% coverage: 9:305/319 of query aligns to 7:290/294 of 2ej2A

query
sites
2ej2A

I

I

W

W

Y

M

N

N

G

G

K

A

L

F

V

V

P

P

W

Q

E

E

K

E

A

A

T

K

V

T

H

S

V

V

L

L

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

E

T

T

A

A

Q

K

G

G

P

P

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

R

K

I

V

Y

L

R

R

I

M

D

E

I

I

P

P

Y

F

T

A

P

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

K

K

E

E

I

V

I

V

V

R

V

R

N

N

G

G

L

Y

D

-

K

R

G

S

A

C

Y
|
Y

M

I

R

R

P

P

F

L

A

A

F

W

R

M

S

G

Y

A

G

K

E

A

I

L

G

G

V

V

A

N

P

P

K

L

T

P

P

N

P

N

P

P

I

A

D

E

T

V

V

M

V

V

A

A

A

A

F

W

E

E

W

W

G

G

A

A

Y

R

L

L

G

-

A

-

D

-

G

-

L

-

E

-

N

-

G

-

I

-

D

-

V

-

C

I

V

T

S

S

S

S

W

W

Q

A

R

R

L

F

A

P

A

A

N

N

T

V

I

M

P

P

A

G

L

K

G

A

K
|
K

A

V

A

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

S

K

M

M

E

E

A

A

K

V

R

A

L

A

G

G

F

A

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

E

D

E

G

G

S

Y

V

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

V

F

V

V

R

R

D

D

N

G

V

V

I

I

Y

Y

T

-

P

-

G

A

L

L

A

E

N

H

A

S

I

V

-

N

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

D

T

S

V

V

I

I

T

R

L

I

A

A

R

K

I

D

H

L

G

G

F

Y

E

E

V

V

R

Q

E

V

Q

V

A

R

L

A

P

T

R

R

E

D

F

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

I

V

F

F

L

M

T

T

G

G

S
x
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

I

R

E

P

V

I

G

G

S

K

G

G

K

T

R

A

G

G

P

P

V

V

T

A

E

L

A

R

L

L

Q

R

S

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

K

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K147 (= K161), E181 (= E195), L204 (= L219)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y97), Y152 (= Y166), E181 (= E195), G184 (= G198), E185 (= E199), L204 (= L219), G206 (= G221), I207 (= I222), T208 (= T223), T244 (≠ S259), A245 (= A260)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
44% identity, 93% coverage: 9:305/319 of query aligns to 7:293/297 of 2ej3A

query
sites
2ej3A

I

I

W

W

Y

M

N

N

G

G

K

A

L

F

V

V

P

P

W

Q

E

E

K

E

A

A

T

K

V

T

H

S

V

V

L

L

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

E

T

T

A

A

Q

K

G

G

P

P

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

R

K

I

V

Y

L

R

R

I

M

D

E

I

I

P

P

Y

F

T

A

P

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

K

K

E

E

I

V

I

V

V

R

V

R

N

N

G

G

L

Y

D

-

K

R

G

S

A

C

Y

Y

M

I

R

R

P

P

F

L

A

A

F

W

R

M

S

G

Y

A

G

K

E

A

I

L

G

G

V

V

A

N

P

P

K

L

T

P

P

N

P

N

P

P

I

A

D

E

T

V

V

M

V

V

A

A

A

A

F

W

E

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

D

-

G

-

L

V

E

R

N

K

G

G

I

A

D

R

V

L

C

I

V

T

S

S

S

S

W

W

Q

A

R

R

L

F

A

P

A

A

N

N

T

V

I

M

P

P

A

G

L

K

G

A

K
|
K

A

V

A

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

S

K

M

M

E

E

A

A

K

V

R

A

L

A

G

G

F

A

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

E

D

E

G

G

S

Y

V

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

V

F

V

V

R

R

D

D

N

G

V

V

I

I

Y

Y

T

-

P

-

G

A

L

L

A

E

N

H

A

S

I

V

-

N

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

D

T

S

V

V

I

I

T

R

L

I

A

A

R

K

I

D

H

L

G

G

F

Y

E

E

V

V

R

Q

E

V

Q

V

A

R

L

A

P

T

R

R

E

D

F

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

I

V

F

F

L

M

T

T

G
|
G

S
x
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

I

R

E

P

V

I

G

G

S

K

G

G

K

T

R

A

G

G

P

P

V

V

T

A

E

L

A

R

L

L

Q

R

S

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

K

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K161), E184 (= E195), L207 (= L219)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G198), G246 (= G258), T247 (≠ S259), A248 (= A260)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G258), T247 (≠ S259)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
44% identity, 93% coverage: 9:305/319 of query aligns to 7:293/297 of 2eiyA

query
sites
2eiyA

I

I

W

W

Y

M

N

N

G

G

K

A

L

F

V

V

P

P

W

Q

E

E

K

E

A

A

T

K

V

T

H

S

V

V

L

L

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

E

T

T

A

A

Q

K

G

G

P

P

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

R

K

I

V

Y

L

R

R

I

M

D

E

I

I

P

P

Y

F

T

A

P

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

K

K

E

E

I

V

I

V

V

R

V

R

N

N

G

G

L

Y

D

-

K

R

G

S

A

C

Y
|
Y

M

I

R

R

P

P

F

L

A

A

F

W

R

M

S

G

Y

A

G

K

E

A

I

L

G

G

V

V

A

N

P

P

K

L

T

P

P

N

P

N

P

P

I

A

D

E

T

V

V

M

V

V

A

A

A

A

F

W

E

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

D

-

G

-

L

V

E

R

N

K

G

G

I

A

D

R

V

L

C

I

V

T

S

S

S

S

W

W

Q

A

R

R

L

F

A

P

A

A

N

N

T

V

I

M

P

P

A

G

L

K

G

A

K
|
K

A

V

A

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

S

K

M

M

E

E

A

A

K

V

R

A

L

A

G

G

F

A

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

E

D

E

G

G

S

Y

V

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

V

F

V

V

R

R

D

D

N

G

V

V

I

I

Y

Y

T

-

P

-

G

A

L

L

A

E

N

H

A

S

I

V

-

N

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

D

T

S

V

V

I

I

T

R

L

I

A

A

R

K

I

D

H

L

G

G

F

Y

E

E

V

V

R

Q

E

V

Q

V

A

R

L

A

P

T

R

R

E

D

F

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

I

V

F

F

L

M

T

T

G
|
G

S
x
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

I

R

E

P

V

I

G

G

S

K

G

G

K

T

R

A

G

G

P

P

V

V

T

A

E

L

A

R

L

L

Q

R

S

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

K

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K161), E184 (= E195), L207 (= L219)
binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y97), T247 (≠ S259), A248 (= A260)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), E188 (= E199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G258), T247 (≠ S259)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
44% identity, 93% coverage: 9:305/319 of query aligns to 7:293/297 of 1wrvA

query
sites
1wrvA

I

I

W

W

Y

M

N

N

G

G

K

A

L

F

V

V

P

P

W

Q

E

E

K

E

A

A

T

K

V

T

H

S

V

V

L

L

A

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

T

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

E

T

T

A

A

Q

K

G

G

P

P

V

A

I

I

F

F

R

R

L

L

R

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

R

K

I

V

Y

L

R

R

I

M

D

E

I

I

P

P

Y

F

T

A

P

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

K

K

E

E

I

V

I

V

V

R

V

R

N

N

G

G

L

Y

D

-

K

R

G

S

A

C

Y

Y

M

I

R

R

P

P

F

L

A

A

F

W

R

M

S

G

Y

A

G

K

E

A

I

L

G

G

V

V

A

N

P

P

K

L

T

P

P

N

P

N

P

P

I

A

D

E

T

V

V

M

V

V

A

A

A

A

F

W

E

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

D

-

G

-

L

V

E

R

N

K

G

G

I

A

D

R

V

L

C

I

V

T

S

S

S

S

W

W

Q

A

R

R

L

F

A

P

A

A

N

N

T

V

I

M

P

P

A

G

L

K

G

A

K
|
K

A

V

A

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

S

K

M

M

E

E

A

A

K

V

R

A

L

A

G

G

F

A

A

D

E

E

G

A

I

L

G

L

L

L

G

D

I

E

D

E

G

G

S

Y

V

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

V

F

V

V

R

R

D

D

N

G

V

V

I

I

Y

Y

T

-

P

-

G

A

L

L

A

E

N

H

A

S

I

V

-

N

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

D

T

S

V

V

I

I

T

R

L

I

A

A

R

K

I

D

H

L

G

G

F

Y

E

E

V

V

R

Q

E

V

Q

V

A

R

L

A

P

T

R

R

E

D

F

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

I

V

F

F

L

M

T

T

G

G

S
x
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

I

R

E

P

V

I

G

G

S

K

G

G

K

T

R

A

G

G

P

P

V

V

T

A

E

L

A

R

L

L

Q

R

S

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

K

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K161), E184 (= E195), L207 (= L219)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), T247 (≠ S259)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
39% identity, 95% coverage: 4:305/319 of query aligns to 4:297/301 of 6thqB

query
sites
6thqB

Q

H

A

G

A

S

K

M

H

K

I

V

W

W

Y

L

N

D

G

G

K

R

L

L

V

V

P

D

W

E

E

E

K

E

A

A

T

K

V

V

H

T

V

V

L

L

A

S

H

P

A

S

L

L

H

N

Y

Y

G

G

S

F

T

G

V

V

F
|
F

E

E

G

G

V

I

R

R

C

A

Y

Y

A

W

T

N

A

G

Q

E

G

N

P

L

V

Y

I

V

F

F

R

R

L

L

R

R

D

D

H

H

T

M

R

E

R
|
R

L

L

F

L

D

R

S

S

A

A

R

K

I

I

Y

I

R

G

I

L

D

D

I

V

P

P

Y

Y

T

T

P

A

D

E

E

E

I

L

N

S

A

K

A

A

C

V

K

V

E

E

I

T

I

V

V

R

V

A

N

N

G

G

L

F

D

K

K

E

G

D

A

L

Y
|
Y

M

I

R

R

P

P

F

V

A

A

F

Y

R

I

S

S

Y

K

G

P

E

Q

I

I

G

S

V

L

A

D

P

V

K

R

T

G

P

L

P

Q

P

A

I

-

D

S

T

V

V

A

V

I

A

A

A

A

F

I

E

P

W

F

G

G

A

K

Y

Y

L

L

G

K

A

V

D

E

G

G

L

-

E

-

N

-

G

-

I

V

D

R

V

A

C

A

V

V

S

V

S

S

W

W

Q

R

R

R

L

V

A

H

A

T

N

S

T

M

I

M

P

P

A

V

L

M

G

A

K
|
K

A

A

A

T

G

G

N

I

Y
|
Y

L

L

S

N

S

S

Q

I

L

M

I

A

S

A

M

V

E

E

A

A

K

R

R

A

L

R

G

G

F

Y

A

D

E

E

G

A

I

I

G

M

L

L

G

N

I

A

D

E

G

G

S

K

V

V

S

V

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

I

F

F

V

I

V

V

R

R

D

R

N

G

V

V

I

L

Y

M

T

T

P

P

G

P

L

L

A

E

N

D

A

G

I

I

L
|
L

Q

E

G
|
G

I
|
I

T
|
T

R

R

D

E

T

T

V

V

I

I

T

S

L

I

A

A

R

G

I

D

H

L

G

G

F

I

E

P

V

L

R

L

E

E

Q

K

A

S

L

I

P

T

R

R

E

E

F

E

L

L

Y

Y

L

A

A

A

D

D

E

E

I

A

F

F

L

F

T

V

G
|
G

S
x
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

I

R

I

S

E

V

I

D

D

-

G

R

R

I

V

E

L

V

-

G

-

S

-

G

-

K

Q

R

R

G

G

P

P

V

I

T

T

E

Q

A

K

L

I

Q

A

S

E

A

T

F

Y

F

R

G

R

L

I

F

V

D

L

G

G

K

K

T

E

E

E

D

K

K

Y

W

L

G

P

W

W

L

L

active site: F37 (= F37), K156 (= K161), E190 (= E195), L214 (= L219)
binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S259)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y97), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (≠ S259), A255 (= A260)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
36% identity, 94% coverage: 7:305/319 of query aligns to 4:301/307 of 6q8eA

query
sites
6q8eA

K

K

H

Y

I

L

W

W

Y

W

N

N

G

H

K

R

L

I

V

I

P

P

W

W

E

E

K

E

A

A

T

T

V

V

H

H

V

L

L

T

A

D

H

Y

A

W

L

W

H

A

Y

S

G

V

S

T

T

A

V

V

F
|
F

E

E

G

G

V

I

R

R

C

G

Y

Y

-

W

A

N

T

N

A

A

Q

E

G

G

P

E

V

M

-

Y

I

I

F

F

R

R

L

L

R

E

D

D

H

H

T

A

R

R

R
|
R

L

L

F

E

D

Q

S

S

A

M

R

Q

I

L

Y

I

R

R

I

M

D

P

I

K

P

E

Y

F

T

T

P

V

D

D

E

E

I

I

N

C

A

Q

A

A

C

T

K

I

E

D

I

L

I

V

V

R

V

A

N

N

G

D

L

Y

D

R

K

G

G

D

A

V

Y

Y

M

I

R

M

P

P

F

L

A

A

F

Y

-

A

-

V

-

G

-

N

R

K

S

A

Y

F

G

S

E

V

I

V

G

G

V

-

A

-

P

-

K

-

T

-

P

-

P

-

P

-

I

-

D

D

T

R

V

T

V

T

A

E

A

M

F

F

E

I

W

Y

G

S

A

R

Y

P

L

A

G

V

A

S

D

R

G

L

L

E

E

E

N

D

-

F

G

S

I

L

D

H

V

A

C

C

V

Y

S

S

S

S

W

W

Q

T

R

R

L

I

A

N

A

E

N

R

T

V

I

L

P

P

A

P

L

R

G

I

K
|
K

A

A

A

L

G

A

N

N

Y
|
Y

L

R

S

N

S

S

Q

Q

L

L

I

A

S

S

M

S

E

E

A

A

K

A

R

M

L

N

G

G

F

Y

A

D

E

T

G

A

I

L

G

F

L

L

G

N

I

P

D

E

G

G

S

K

V

V

S

A

E
|
E

G

G

A

T

G
|
G

E
x
S

N
x
C

L

V

F

F

V

F

V

V

R

R

D

K

N

G

V

K

I

L

Y

I

T

T

P

P

G

D

L

I

A

T

N

S

A

G

I

I

L
|
L

Q

E

G
x
S

I
|
I

T
|
T

R

R

D

D

T

T

V

V

I

I

T

H

L

L

A

A

R

R

-

E

I

V

H

L

G

G

F

L

E

E

V

V

R

E

E

E

Q

R

A

V

L

V

P

D

R

R

E

T

F

E

L

T

Y

Y

L

L

A

A

D

D

E

E

I

A

F

F

L

L

T

C

G
|
G

S
x
T

A

H

A

A

E

E

I

I

T

T

P

P

V

I

R

A

S

S

V

I

D

D

R

R

I

H

E

E

V

M

G

K

S

H

G

G

K

A

R

P

G

G

P

P

V

I

T

T

E

R

A

Q

L

L

Q

R

S

D

A

I

F

Y

F

R

G

E

L

V

F

V

D

Y

G

G

K

R

T

D

E

F

D

R

K

Y

W

R

G

N

W

W

L

L

active site: F34 (= F37), K156 (= K161), E190 (= E195), L214 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), C195 (≠ N200), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), G254 (= G258), T255 (≠ S259)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
34% identity, 93% coverage: 10:305/319 of query aligns to 8:303/308 of 6h65C

query
sites
6h65C

W

W

Y

M

N

N

G

G

K

Q

L

C

V

I

P

P

W

W

E

D

K

Q

A

C

T

S

V

L

H

H

V

V

L

S

A

T

H

Q

A

A

L

A

H

F

Y

F

G

G

S

A

T

S

V

L

F
|
F

E

E

G

G

V

V

R

R

C

A

Y

Y

A

W

T

N

A

A

Q

E

G

R

P

E

-

Q

-

L

V

Y

I

V

F

F

R

R

L

L

R

D

D

E

H

H

T

L

R

R

R
|
R

L

L

F

E

D

Q

S

S

A

A

R

K

I

M

Y

L

R

R

I

M

D

K

I

L

P

S

Y

M

T

P

P

I

D

A

E

D

I

I

N

R

A

Q

A

G

C

V

K

L

E

E

I

L

I

L

V

R

V

A

N

N

G

E

L

F

D

R

K

S

G

D

A

V

Y

H

M

L

R

-

P

-

F

-

A

Y

F

V

R

A

S

S

Y

Y

G

F

E

G

I

I

G

N

V

H

A

D

P

P

K

D

T

P

P

L

P

F

P

P

I

T

D

D

T

D

V

T

V

G

A

V

A

Y

F

V

E

T

W

G

G

T

A

A

Y

V

L

S

G

R

A

L

D

P

G

L

L

V

E

H

N

T

G

G

I

I

D

S

V

A

C

C

V

M

S

S

S

S

W

W

Q

R

R

R

L

I

A

S

A

D

N

D

T

S

I

V

P

P

A

P

L

R

G

I

K
|
K

A

I

A

G

G

A

N

N

Y
|
Y

L

Q

S

N

S

S

Q

R

L

L

I

A

S

Q

M

T

E

E

A

A

K

R

R

V

L

N

G

G

F

Y

A

H

E

T

G

S

I

V

G

L

L

L

G

N

I

S

D

R

G

G

S

K

V

V

S

S

E
|
E

G

T

A

P

G

G

E
x
A

N

C

L

L

F

L

V

M

V

V

R

R

D

D

N

G

V

R

I

V

Y

I

T

S

P

P

G

P

L

V

A

T

N

A

A

D

I

I

L
|
L

Q

E

G
x
S

I
x
V

T
|
T

R

R

D

K

T

T

V

L

I

M

T

S

L

L

A

S

R

E

I

A

H

E

-

L

G

D

F

S

E

P

V

V

R

I

E

E

Q

R

A

D

L

M

P

D

R

R

E

T

F

E

L

L

Y

Y

L

I

A

A

D

E

E

E

I

V

F

F

L

L

T

C

G
|
G

S
x
T

A

I

A

A

E

E

I

I

T

L

P

P

V

V

R

T

S

T

V

I

D

D

R

R

I

I

E

Q

V

V

G

G

S

D

G

G

K

E

R

V

G

G

P

P

V

V

T

T

E

R

A

R

L

L

Q

Q

S

E

A

L

F

Y

F

F

G

G

L

V

F

T

D

S

G

G

K

Q

T

L

E

E

D

A

K

Y

W

K

G

S

W

W

L

L

active site: F35 (= F37), K158 (= K161), E192 (= E195), L216 (= L219)
binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K161), Y163 (= Y166), E192 (= E195), A196 (≠ E199), L216 (= L219), S218 (≠ G221), V219 (≠ I222), T220 (= T223), G256 (= G258), T257 (≠ S259)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
36% identity, 94% coverage: 7:305/319 of query aligns to 4:301/309 of 7neaA

query
sites
7neaA

K

K

H

Y

I

L

W

W

Y

W

N

N

G

H

K

R

L

I

V

I

P

P

W

W

E

E

K

E

A

A

T

T

V

V

H

H

V

L

L

T

A

D

H

Y

A

W

L

W

H

A

Y

S

G

V

S

T

T

A

V

V

F
|
F

E

E

G

V

V

I

R

S

C

G

Y

Y

-

W

A

N

T

N

A

A

Q

E

G

G

P

E

V

M

-

Y

I

I

F

F

R

R

L

L

R

E

D

D

H

H

T

A

R

R

R
|
R

L

L

F

E

D

Q

S

S

A

M

R

Q

I

L

Y

I

R

R

I

M

D

P

I

K

P

E

Y

F

T

T

P

V

D

D

E

E

I

I

N

C

A

Q

A

A

C

T

K

I

E

D

I

L

I

V

V

R

V

A

N

N

G

D

L

Y

D

R

K

G

G

D

A

V

Y

F

M

I

R

M

P

P

F

L

A

A

F

Y

-

A

-

V

-

G

-

N

R

K

S

A

Y

F

G

S

E

V

I

V

G

G

V

-

A

-

P

-

K

-

T

-

P

-

P

-

P

-

I

-

D

D

T

R

V

T

V

T

A

E

A

M

F

F

E

I

W

Y

G

S

A

R

Y

P

L

A

G

V

A

S

D

R

G

L

L

E

E

E

N

D

-

F

G

S

I

L

D

H

V

A

C

C

V

Y

S

S

S

S

W

W

Q

T

R

R

L

I

A

N

A

E

N

R

T

V

I

L

P

P

A

P

L

R

G

I

K
|
K

A

A

A

L

G

A

N

N

Y
|
Y

L

R

S

N

S

S

Q

Q

L

L

I

A

S

S

M

S

E

E

A

A

K

A

R

M

L

N

G

G

F

Y

A

D

E

T

G

A

I

L

G

F

L

L

G

N

I

P

D

E

G

G

S

K

V

V

S

A

E
|
E

G

G

A

T

G
|
G

E
x
S

N

C

L

V

F

F

V

F

V

V

R

R

D

K

N

G

V

K

I

L

Y

I

T

T

P

P

G

D

L

I

A

T

N

S

A

G

I

I

L
|
L

Q

E

G
x
S

I
|
I

T
|
T

R

R

D

D

T

T

V

V

I

I

T

H

L

L

A

A

R

R

-

E

I

V

H

L

G

G

F

L

E

E

V

V

R

E

E

E

Q

R

A

V

L

V

P

D

R

R

E

T

F

E

L

T

Y

Y

L

L

A

A

D

D

E

E

I

A

F

F

L

L

T

C

G

G

S
x
T

A

H

A

A

E

E

I

I

T

T

P

P

V

I

R

A

S

S

V

I

D

D

R

R

I

H

E

E

V

M

G

K

S

H

G

G

K

A

R

P

G

G

P

P

V

I

T

T

E

R

A

Q

L

L

Q

R

S

D

A

I

F

Y

F

R

G

E

L

V

F

V

D

Y

G

G

K

R

T

D

E

F

D

R

K

Y

W

R

G

N

W

W

L

L

active site: F34 (= F37), K156 (= K161), E190 (= E195), L214 (= L219)
binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), T255 (≠ S259)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
38% identity, 88% coverage: 9:290/319 of query aligns to 5:278/290 of 5e25A

query
sites
5e25A

I

V

W

Y

Y

M

N

N

G

G

K

E

L

F

V

V

P

P

W

E

E

S

K

Q

A

A

T

K

V

V

H

S

V

V

L

F

A

D

H

H

A

G

L

F

H

L

Y

Y

G

G

S

D

T

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

A

N

T

G

A

K

Q

-

G

-

P

-

V

-

I

V

F

F

R

K

L

L

R

Y

D

E

H

H

T

I

R

D

R
|
R

L

L

F

Y

D

D

S

C

A

A

R

R

I

V

Y

I

R

D

I

L

D

K

I

I

P

P

Y

L

T

S

P

K

D

E

E