SitesBLAST
Comparing WP_066921446.1 NCBI__GCF_001579945.1:WP_066921446.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
47% identity, 99% coverage: 3:252/253 of query aligns to 1:250/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G16 (= G18), I17 (= I19), D36 (= D38), L37 (≠ I39), C61 (≠ A63), D62 (= D64), V63 (= V65), N89 (= N91), A90 (= A92), T140 (≠ S142), S142 (= S144), Y155 (= Y157), K159 (= K161), A186 (≠ G188), V187 (= V189)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
43% identity, 98% coverage: 4:252/253 of query aligns to 2:247/248 of 6ixmC
- active site: G16 (= G18), S142 (= S144), Y155 (= Y157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S15 (= S17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), A61 (= A63), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), M140 (≠ S142), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (≠ G188), Y187 (≠ V189), I188 (= I190), L192 (≠ M194)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
44% identity, 98% coverage: 4:252/253 of query aligns to 3:248/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G14), S16 (= S17), G17 (= G18), I18 (= I19), D37 (= D38), I38 (= I39), A62 (= A63), D63 (= D64), S64 (≠ V65), N90 (= N91), M141 (≠ S142), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (= G188), Y188 (≠ V189), I189 (= I190), L193 (≠ M194)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 4:253/253 of query aligns to 2:248/248 of 4urfB
- active site: G16 (= G18), S142 (= S144), I152 (≠ L154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ M215), R211 (≠ G216), R212 (= R217)
- binding bicarbonate ion: I92 (≠ V94), G94 (≠ S96), R109 (= R111), R179 (= R181), S228 (= S233)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (≠ S17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ S142), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (≠ G188), I188 (= I190), T190 (= T192)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 4:253/253 of query aligns to 2:248/248 of 4urfA
- active site: G16 (= G18), S142 (= S144), I152 (≠ L154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (≠ V94), S93 (≠ E95), G94 (≠ S96), E95 (≠ T97), T97 (≠ G99), E101 (≠ Q103), T103 (= T105), Q106 (≠ N108), R109 (= R111), S175 (≠ T177), G177 (≠ N179)
- binding magnesium ion: S237 (≠ H242), Y238 (≠ A243)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (≠ S17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), W41 (≠ A43), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ S142), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 4:253/253 of query aligns to 2:248/248 of 4ureB
- active site: G16 (= G18), S142 (= S144), I152 (≠ L154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ S17), G16 (= G18), I17 (= I19), N89 (= N91), G91 (= G93), Y155 (= Y157), P185 (= P187), A186 (≠ G188)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
40% identity, 98% coverage: 5:253/253 of query aligns to 5:255/255 of 5itvA
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (= I39), T61 (≠ A63), I63 (≠ V65), N89 (= N91), G91 (= G93), T139 (≠ S142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (= G188), I186 (≠ V189), I187 (= I190)
4qecA Elxo with NADP bound (see paper)
36% identity, 97% coverage: 8:253/253 of query aligns to 3:248/248 of 4qecA
- active site: G13 (= G18), N111 (= N116), S139 (= S144), Y152 (= Y157), K156 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ S17), G13 (= G18), I14 (= I19), S33 (≠ D38), R34 (≠ I39), K38 (≠ A43), D59 (= D64), V60 (= V65), N86 (= N91), A87 (= A92), G88 (= G93), I137 (≠ S142), Y152 (= Y157), K156 (= K161), P182 (= P187), I185 (= I190)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
39% identity, 98% coverage: 5:253/253 of query aligns to 5:227/227 of 5itvD
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (= I39), T61 (≠ A63), D62 (= D64), I63 (≠ V65), N89 (= N91), T139 (≠ S142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (= G188), I187 (= I190)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 97% coverage: 5:249/253 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G18), N111 (= N116), S139 (= S144), Q149 (≠ L154), Y152 (= Y157), K156 (= K161)
- binding acetoacetyl-coenzyme a: D93 (≠ P98), K98 (≠ Q103), S139 (= S144), N146 (≠ F151), V147 (≠ P152), Q149 (≠ L154), Y152 (= Y157), F184 (≠ V189), M189 (= M194), K200 (≠ A208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (≠ S17), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ I39), V59 (≠ A63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (≠ V94), T137 (≠ S142), S139 (= S144), Y152 (= Y157), K156 (= K161), P182 (= P187), F184 (≠ V189), T185 (≠ I190), T187 (= T192), M189 (= M194)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
37% identity, 98% coverage: 1:249/253 of query aligns to 4:249/258 of 4wecA
- active site: G21 (= G18), S143 (= S144), Q154 (≠ L154), Y157 (= Y157), K161 (= K161)
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), A19 (= A16), S20 (= S17), G21 (= G18), I22 (= I19), D41 (= D38), I42 (= I39), V61 (≠ A63), D62 (= D64), V63 (= V65), N89 (= N91), T141 (≠ S142), Y157 (= Y157), K161 (= K161), P187 (= P187), P189 (≠ V189), V190 (≠ I190)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 98% coverage: 5:251/253 of query aligns to 3:246/247 of 4jroC
- active site: G16 (= G18), S142 (= S144), Q152 (≠ L154), Y155 (= Y157), K159 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ A16), R15 (≠ S17), G16 (= G18), I17 (= I19), N35 (vs. gap), Y36 (≠ A37), N37 (≠ D38), G38 (≠ I39), S39 (≠ Q40), N63 (≠ D64), V64 (= V65), N90 (= N91), A91 (= A92), I93 (≠ V94), I113 (= I115), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
41% identity, 97% coverage: 8:252/253 of query aligns to 14:256/257 of 5h5xC
- active site: G24 (= G18), S151 (= S144), Y164 (= Y157), K168 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), S23 (= S17), G24 (= G18), I25 (= I19), D44 (= D38), F45 (≠ I39), L69 (≠ A63), D70 (= D64), N97 (= N91), A98 (= A92), Y164 (= Y157), K168 (= K161), P194 (= P187), G195 (= G188), I197 (= I190), T199 (= T192)
8w0oA Gdh-105 crystal structure
40% identity, 97% coverage: 5:249/253 of query aligns to 5:249/259 of 8w0oA
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), L19 (≠ I19), Y39 (≠ I39), Q43 (≠ A43), D65 (= D64), V66 (= V65), N92 (= N91), A93 (= A92), G94 (= G93), M143 (≠ S142), Y158 (= Y157), K162 (= K161), P188 (= P187), G189 (= G188), I191 (= I190), T193 (= T192)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
40% identity, 98% coverage: 1:249/253 of query aligns to 7:255/267 of 3ay6B
- active site: G24 (= G18), S151 (= S144), Y164 (= Y157), K168 (= K161)
- binding beta-D-glucopyranose: E102 (= E95), S151 (= S144), H153 (≠ A146), W158 (≠ F151), Y164 (= Y157), N202 (≠ R197), K205 (≠ H200)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), T23 (≠ S17), G24 (= G18), L25 (≠ I19), Y45 (≠ I39), D71 (= D64), V72 (= V65), N98 (= N91), A99 (= A92), G100 (= G93), V101 (= V94), M149 (≠ S142), S151 (= S144), Y164 (= Y157), K168 (= K161), P194 (= P187), G195 (= G188), M197 (≠ I190), T199 (= T192), P200 (≠ A193), I201 (≠ M194), N202 (≠ R197)
P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
39% identity, 98% coverage: 1:249/253 of query aligns to 1:249/261 of P40288
- 11:35 (vs. 11:35, 44% identical) binding NADP(+)
- E96 (= E95) mutation E->A,G,K: Heat stable.
- D108 (≠ N108) mutation to N: Heat stable.
- V112 (≠ T112) mutation to A: Heat stable.
- E133 (≠ P129) mutation to K: Heat stable.
- V183 (= V182) mutation to I: Heat stable.
- P194 (≠ A193) mutation to Q: Heat stable.
- E210 (≠ A210) mutation to K: Heat stable.
- Y217 (≠ R217) mutation to H: Heat stable.
Sites not aligning to the query:
- 252 Q→L: Heat stable.
- 253 Y→C: Heat stable.
- 258 A→G: Heat stable.
1iy8A Crystal structure of levodione reductase (see paper)
37% identity, 98% coverage: 5:252/253 of query aligns to 2:256/258 of 1iy8A
- active site: G15 (= G18), S143 (= S144), Q153 (≠ L154), Y156 (= Y157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (= S17), G15 (= G18), L16 (≠ I19), D35 (= D38), V36 (≠ I39), A62 (= A63), D63 (= D64), V64 (= V65), N90 (= N91), G92 (= G93), I93 (≠ V94), T141 (≠ S142), S143 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (= G188), T191 (= T192), P192 (≠ A193), M193 (= M194)
3toxA Crystal structure of a short chain dehydrogenase in complex with NAD(p) from sinorhizobium meliloti 1021
42% identity, 97% coverage: 5:249/253 of query aligns to 3:249/254 of 3toxA
- active site: G16 (= G18), S142 (= S144), V153 (≠ L154), Y156 (= Y157), K160 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ A16), S15 (= S17), G16 (= G18), I17 (= I19), A36 (≠ D38), R37 (≠ I39), N38 (≠ Q40), V63 (= V65), N89 (= N91), A90 (= A92), G91 (= G93), T140 (≠ S142), S142 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G188 (≠ V189), T189 (≠ I190), T191 (= T192)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
38% identity, 98% coverage: 5:252/253 of query aligns to 11:265/267 of Q9LBG2
- 17:42 (vs. 11:36, 50% identical) binding NAD(+)
- E103 (= E95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
37% identity, 97% coverage: 5:250/253 of query aligns to 5:251/254 of 4fn4A
- active site: G18 (= G18), S144 (= S144), Y157 (= Y157), K161 (= K161), S202 (≠ E202)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (= S17), G18 (= G18), I19 (= I19), E38 (≠ D38), L39 (≠ I39), R43 (≠ A43), A63 (= A63), D64 (= D64), V65 (= V65), N91 (= N91), G93 (= G93), I94 (≠ V94), T142 (≠ S142), S144 (= S144), Y157 (= Y157), K161 (= K161), P187 (= P187), V190 (≠ I190), T192 (= T192), N193 (≠ A193), I194 (≠ M194)
Query Sequence
>WP_066921446.1 NCBI__GCF_001579945.1:WP_066921446.1
MKNMLKDKVVLVTGGASGIGAVAANAFAREGAAVVLADIQLEAGAKIAESICDAGGKALF
VKADVTLEADVKDMVTTTVKTFGRLDCAFNNAGVESTPGDTVQCTNENWDRTININLRGI
WWCMKYEIPEMIKTGGGAIVNSSSIAGMIAFPGLPAYVASKHGVIGLSKNAALEFATQNI
RVNALCPGVIHTAMIDRLTHGEEQAERAMAEGAPMGRMGTPEEIARTALWLCSDESSFVT
GHALVADGGWVAR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory