SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066921571.1 NCBI__GCF_001579945.1:WP_066921571.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

D2Z027 O-ureido-L-serine synthase; Cysteine synthase homolog DscD; O-acetylserine sulfhydrylase; EC 2.6.99.3; EC 2.5.1.47 from Streptomyces lavendulae (see paper)
59% identity, 98% coverage: 2:310/314 of query aligns to 3:311/324 of D2Z027

query
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D2Z027

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K43 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Loss of catalytic activity, no longer binds N6-(pyridoxal phosphate)lysine.
V74 (≠ T73) mutation to T: KM for OAS is 61 mM, KM for H(2)S is unchanged.
Y97 (≠ F96) mutation to F: KM for OAS is unchanged, KM for H(2)S is 0.073 mM.; mutation to M: KM for OAS is 330 mM, KM for H(2)S is 0.084.
S121 (≠ A120) mutation to A: KM for OAS is 140 mM, KM for H(2)S is 0.095 mM.; mutation to M: KM for OAS is 44 mM, KM for H(2)S is 0.20 mM.

3x43A Crystal structure of o-ureido-l-serine synthase (see paper)
59% identity, 98% coverage: 2:310/314 of query aligns to 2:310/316 of 3x43A

query
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active site: K42 (= K42), S264 (= S264)
binding pyridoxal-5'-phosphate: K42 (= K42), N72 (= N72), F175 (≠ W175), G176 (= G176), T177 (= T177), T178 (≠ G178), T180 (= T180), G220 (= G220), S264 (= S264), P290 (= P290), D291 (= D291)

3x44A Crystal structure of o-ureido-l-serine-bound k43a mutant of o-ureido- l-serine synthase (see paper)
58% identity, 98% coverage: 2:310/314 of query aligns to 2:310/321 of 3x44A

query
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3x44A

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active site: A42 (≠ K42), S264 (= S264)
binding (E)-O-(carbamoylamino)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: T69 (= T69), S70 (= S70), N72 (= N72), V73 (≠ T73), S120 (≠ A120), Q141 (= Q141), F175 (≠ W175), G176 (= G176), T177 (= T177), T178 (≠ G178), T180 (= T180), G220 (= G220), L221 (≠ W221), P223 (= P223), S264 (= S264), P290 (= P290), D291 (= D291)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 97% coverage: 2:307/314 of query aligns to 3:310/310 of P9WP55

query
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Q

Q

V

V

A

A

Q

R

G

R

A

P

P

E

Q

N

-

A

G

G

S

K

I

L

I

I

L

V

A

V

M

V

L

L

P

P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

E

A

G

D

I

V

N

A

E

D

K44 (= K42) modified: N6-(pyridoxal phosphate)lysine
N74 (= N72) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 176:180) binding pyridoxal 5'-phosphate
S266 (= S264) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
49% identity, 96% coverage: 2:302/314 of query aligns to 3:305/306 of 2q3dA

query
sites
2q3dA

I

I

Y

A

D

E

N

D

I

I

L

T

G

Q

T

L

I

I

G

G

R

R

T

T

P

P

I

L

V

V

K

R

I

L

N

R

R

R

L

V

A

T

P

D

A

G

H

A

V

V

-

A

E

D

M

I

Y

V

V

A

K

K

C

L

E

E

F

F

N

N

P

P

L

A

A

N

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

V

A

A

I

M

I

L

E

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

V

L

L

I

K

K

P

P

G

D

Q

T

T

I

V

I

V

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

C

C

A

A

K

R

G

G

Y

Y

P

R

F

C

V

V

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

L

E

E

R

R

K

M

V

L

M

L

R

R

M

A

L

Y

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

G

D

R

G

A

M

S

S

G

G

M

A

V

I

V

A

K

K

A

A

A

E

E

E

L

L

A

A

Q

K

-

T

K

D

H

Q

G

R

W

Y

F

F

L

V

A

P

R

Q

Q
|
Q

F

F

E

E

N

N

E

P

A

A

N

N

P

P

A

A

Y

I

H

H

R

R

N

V

T

T

G

A

P

E

E

E

I

V

L

W

S

R

D

D

F

T

A

D

G

G

R

-

R

K

L

V

D

D

F

I

W

V

V

V

S

A

G

G

W
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

A

V

G

A

E

Q

I

V

I

I

K

K

K

E

A

R

R

K

P

P

Q

S

T

A

R

R

I

F

I

V

A

A

A

V

E

E

P

P

A

A

N

A

A

S

S

P

L

V

L

L

T

S

G

G

A

G

E

Q

F

K

K

G

S

P

H

H

K

P

V

I

Q

Q

G
|
G

W
x
I

T

G

P

A

D

G

F

F

V

V

P

P

A

P

V

V

L

L

N

D

R

Q

S

D

V

L

C

V

D

D

Q

E

I

I

V

I

T

T

V

V

T

G

D

N

D

E

A

D

A

A

I

L

Q

N

Y

V

S

A

R

R

E

R

L

L

A

A

S

R

K

E

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

F

T

A

V

A

A

L

L

Q

Q

V

V

A

A

Q

R

G

R

A

P

P

E

Q

N

-

A

G

G

S

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K42), T71 (= T69), S72 (= S70), N74 (= N72), T75 (= T73), Q144 (= Q141), V177 (≠ W175), G178 (= G176), T179 (= T177), G180 (= G178), T182 (= T180), G222 (= G220), I223 (≠ W221), S266 (= S264), P293 (= P290), D294 (= D291)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
51% identity, 97% coverage: 2:305/314 of query aligns to 4:309/310 of 5xoqA

query
sites
5xoqA

I

I

Y

F

D

K

N

D

I

N

L

S

G

E

T

S

I

I

G

G

R

R

T

T

P

P

I

L

V

V

K

Q

I

I

N

N

R

R

L

L

-

T

A

A

P

G

A

L

H

S

V

S

E

R

M

V

Y

L

V

A

K

K

C

I

E

E

F

G

F

R

N

N

P

P

L

A

A

Y

S

S

V

V

K

K

D

C

R

R

L

I

A

G

I

A

A

A

I

M

I

I

E

W

D

D

A

A

E

E

Q

Q

R

S

G

G

V

K

L

L

K

K

P

P

G

G

Q

M

T

H

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

F

V

V

C

C

A

A

K

R

G

G

Y

Y

P

K

F

L

V

T

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

I

E

E

R

R

K

M

V

M

M

L

R

K

M

S

L

F

G

G

A

A

K

D

V

L

V

V

L

L

T

T

P

P

A

G

A

A

G

D

R

G

A
x
M

S

K

G

G

M

A

V

I

V

S

K

K

A

A

A

E

E

E

L

L

A

A

Q

A

K

Q

H

P

G

G

W

W

F

F

L

I

A

P

R

Q

Q
|
Q

F

F

E

K

N

N

E

P

A

A

N

N

P

P

A

A

Y

I

H

H

R

V

N

K

T

T

G

G

P

P

E

E

I

I

L

W

S

N

D

D

F

T

A

E

G

G

R

-

R

Q

L

V

D

D

F

V

W

F

V

V

S

A

G

G

W

V

G

G

T

T

G

G

T

T

L

I

T

T

G

G

A

V

G

A

E

R

I

F

I

L

K

K

K

H

A

E

R

K

P

K

Q

H

-

P

T

V

R

H

I

V

A

A

A

V

E

E

P

P

A

A

N

A

A

S

S

P

L

V

L

L

T

A

G

G

A

G

E

P

F

A

K

G

S
x
R

H
|
H

K

K

V

I

Q
|
Q

G
|
G

W

I

T

G

P
x
A

D

G

F

F

V

V

P

P

A

D

V

T

L

F

N

D

R

R

S

S

V

V

C

V

D

D

Q

E

I

I

V

L

T

S

V

V

T

T

D

D

A

E

A

A

I

I

Q

E

Y

T

S

A

R

R

E

K

L

L

A

A

S

M

K

E

E

E

G

G

I

I

F

S

C

C

G

G

I

I

S

S

S

C

G

G

A

A

T

A

F

M

A

A

A

G

A

A

L

L

Q

K

V

V

A

A

Q

-

G

A

A

R

P

P

Q

E

-

F

-

A

G

G

S

K

I

T

I

I

L

V

A

T

M

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

E

E

G

N

I

L

binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), M123 (≠ A120), Q144 (= Q141), R218 (≠ S215), H219 (= H216), Q222 (= Q219), G223 (= G220), A226 (≠ P223)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
48% identity, 94% coverage: 2:297/314 of query aligns to 3:300/300 of 3zeiA

query
sites
3zeiA

I

I

Y

A

D

E

N

D

I

I

L

T

G

Q

T

L

I

I

G

G

R

R

T

T

P

P

I

L

V

V

K

R

I

L

N

R

R

R

L

V

A

T

P

D

A

G

H

A

V

V

-

A

E

D

M

I

Y

V

V

A

K

K

C

L

E

E

F

F

N

N

P

P

L

A

A

N

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

V

A

A

I

M

I

L

E

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

V

L

L

I

K

K

P

P

G

D

Q

T

T

I

V

I

V

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

C

C

A

A

K

R

G

G

Y

Y

P

R

F

C

V

V

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

L

E

E

R

R

K

M

V

L

M

L

R

R

M

A

L

Y

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

G

D

R

G

A

M

S

S

G

G

M

A

V
x
I

V

A

K

K

A

A

A

E

E

E

L

L

A

A

Q

K

-

T

K

D

H

Q

G

R

W

Y

F

F

L

V

A

P

R

Q

Q
|
Q

F
|
F

E

E

N

N

E

P

A

A

N

N

P

P

A

A

Y

I

H

H

R

R

N

V

T

T

G

A

P

E

E

E

I

V

L

W

S

R

D

D

F

T

A

D

G

G

R

-

R

K

L

V

D

D

F

I

W

V

V

V

S

A

G

G

W
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

A

V

G

A

E

Q

I

V

I

I

K

K

K

E

A

R

R

K

P

P

Q

S

T

A

R

R

I

F

I

V

A

A

A

V

E

E

P

P

A

A

N

A

A

S

S

P

L

V

L

L

T

S

G

G

A

G

E

Q

F
x
K

K

G

S

P

H

H

K

P

V

I

Q

Q

G
|
G

W

I

T

G

P
x
A

D

G

F
|
F

V

V

P

P

A

P

V

V

L

L

N

D

R

Q

S

D

V

L

C

V

D

D

Q

E

I

I

V

I

T

T

V

V

T

G

D

N

D

E

A

D

A

A

I

L

Q

N

Y

V

S

A

R

R

E

R

L

L

A

A

S

R

K

E

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

F

T

A

V

A

A

L

L

Q

Q

V

V

A

A

Q

R

G

R

A

P

P

E

Q

N

-

A

G

G

S

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T69), S72 (= S70), I126 (≠ V124), Q144 (= Q141), F145 (= F142), K215 (≠ F213), G222 (= G220), A225 (≠ P223), F227 (= F225)
binding pyridoxal-5'-phosphate: K44 (= K42), N74 (= N72), V177 (≠ W175), G178 (= G176), T179 (= T177), G180 (= G178), T182 (= T180), G222 (= G220), S266 (= S264), P293 (= P290), D294 (= D291)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
48% identity, 94% coverage: 2:297/314 of query aligns to 3:300/300 of 2q3cA

query
sites
2q3cA

I

I

Y

A

D

E

N

D

I

I

L

T

G

Q

T

L

I

I

G

G

R

R

T

T

P

P

I

L

V

V

K

R

I

L

N

R

R

R

L

V

A

T

P

D

A

G

H

A

V

V

-

A

E

D

M

I

Y

V

V

A

K

K

C

L

E

E

F

F

N

N

P

P

L

A

A

N

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

V

A

A

I

M

I

L

E

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

V

L

L

I

K

K

P

P

G

D

Q

T

T

I

V

I

V

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

C

C

A

A

K

R

G

G

Y

Y

P

R

F

C

V

V

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

L

E

E

R

R

K

M

V

L

M

L

R

R

M

A

L

Y

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

G

D

R

G

A
x
M

S

S

G

G

M

A

V

I

V

A

K

K

A

A

A

E

E

E

L

L

A

A

Q

K

-

T

K

D

H

Q

G

R

W

Y

F

F

L

V

A

P

R

Q

Q
|
Q

F

F

E

E

N

N

E

P

A

A

N

N

P

P

A

A

Y

I

H

H

R

R

N

V

T

T

G

A

P

E

E

E

I

V

L

W

S

R

D

D

F

T

A

D

G

G

R

-

R

K

L

V

D

D

F

I

W

V

V

V

S

A

G

G

W

V

G

G

T

T

G

G

T

T

L

I

T

T

G

G

A

V

G

A

E

Q

I

V

I

I

K

K

K

E

A

R

R

K

P

P

Q

S

T

A

R

R

I

F

I

V

A

A

A

V

E

E

P

P

A

A

N

A

A

S

S

P

L

V

L

L

T

S

G

G

A

G

E

Q

F
x
K

K

G

S

P

H

H

K

P

V

I

Q

Q

G
|
G

W

I

T

G

P
x
A

D

G

F

F

V

V

P

P

A

P

V

V

L

L

N

D

R

Q

S

D

V

L

C

V

D

D

Q

E

I

I

V

I

T

T

V

V

T

G

D

N

D

E

A

D

A

A

I

L

Q

N

Y

V

S

A

R

R

E

R

L

L

A

A

S

R

K

E

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

F

T

A

V

A

A

L

L

Q

Q

V

V

A

A

Q

R

G

R

A

P

P

E

Q

N

-

A

G

G

S

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding : T71 (= T69), S72 (= S70), G73 (= G71), T75 (= T73), M122 (≠ A120), Q144 (= Q141), K215 (≠ F213), G222 (= G220), A225 (≠ P223)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
43% identity, 97% coverage: 2:307/314 of query aligns to 3:313/318 of 4lmaA

query
sites
4lmaA

I

I

Y

A

D

H

N

D

I

V

L

T

G

E

T

L

I

V

G

G

R

R

T

T

P

P

I

L

V

V

K

Q

I

L

N

N

R

R

L

I

A

P

P

Q

A

A

H

E

-

G

-

C

-

L

V

G

E

R

M

I

Y

V

V

M

K

K

C

L

E

E

F

G

F

M

N

N

P

P

L

A

A

A

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

T

A

H

I

M

I

I

E

N

D

S

A

A

E

E

Q

K

R

A

G

G

V

L

L

I

K

N

P

P

G

E

Q

T

T

T

V

V

-

L

V

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

T

C

A

A

A

K

K

G

G

Y

Y

P

R

F

L

V

I

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

L

E

E

R

R

K

A

V

M

M

L

R

K

M

A

L

Y

G

G

A

A

K

T

V

L

V

E

L

L

T

T

P

P

A

G

A

S

G

Q

R

G

A

M

S

K

G

G

M

A

V

I

V

L

K

R

A

A

A

Q

E

Q

L

I

A

V

Q

D

K

S

-

I

H

P

G

G

W

A

F

Y

L

M

A

L

R

Q

Q

Q

F

F

E

R

N

N

E

P

A

S

N

N

P

P

A

E

Y

I

H

H

R

R

N

L

T

T

G

A

P

E

E

E

I

I

L

W

S

Q

D

D

F

T

A

E

G

G

R

-

R

Q

L

V

D

D

F

F

W

I

V

V

S

A

G

G

W

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

A

V

G

A

E

E

I

V

I

I

K

K

K

S

A

R

R

K

P

P

Q

S

T

F

R

Q

I

V

A

A

A

V

E

E

P

P

A

F

N

N

A

S

S

P

L

V

L

I

T

S

G

G

A

G

E

N

F

P

K

G

S

P

H

H

K

K

V

I

Q

Q

G
|
G

W

I

T

G

P

A

D

G

F

F

V

I

P

P

A

E

V

V

L

L

N

R

R

T

S

D

V

L

C

I

D

D

Q

E

I

V

V

I

T

T

V

V

T

S

D

D

D

E

A

E

A

A

I

F

Q

Q

Y

F

S

G

R

R

E

R

L

L

A

A

S

K

K

E

E

E

G

G

I

L

F

L

C

S

G

G

I

I

S
|
S

S

S

G

G

A

A

T

N

F

L

A

C

A

A

L

I

Q

Q

V

L

A

A

Q

Q

G

R

A

P

P

E

-

N

Q

E

G

G

S

K

I

L

I

I

L

V

A

V

M

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

L

L

M

F

F

E

Q

G

N

I

I

N

E

E

E

active site: K46 (= K42), S269 (= S264)
binding pyridoxal-5'-phosphate: K46 (= K42), N77 (= N72), G181 (= G176), T182 (= T177), G183 (= G178), T185 (= T180), G225 (= G220), S269 (= S264), P296 (= P290)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
44% identity, 97% coverage: 2:307/314 of query aligns to 13:321/323 of 4aecA

query
sites
4aecA

I

I

Y

A

D

D

N

N

I

V

L

S

G

Q

T

L

I

I

G

G

R

K

T

T

P

P

I

M

V

V

K

Y

I

L

N

N

R

S

L

I

A

A

P

K

A

G

H

C

V

V

-

A

E

N

M

I

Y

A

V

A

K

K

C

L

E

E

F

I

F

M

N

E

P

P

L

C

A

C

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

Y

A

S

I

M

I

V

E

T

D

D

A

A

E

E

Q

Q

R

K

G

G

V

F

L

I

K

S

P

P

G

G

Q

K

T

S

V

V

-

L

V

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

C

A

A

A

A

S

K

R

G

G

Y

Y

P

R

F

L

V

I

A

L

V

T

M

M

A

P

E

A

S

S

F

M

S

S

V

M

E

E

R

R

K

V

V

L

M

L

R

K

M

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

D

A

P

A

A

G

K

R

G

A

M

S

T

G

G

M

A

V

V

V

Q

K

K

A

A

A

E

E

E

-

I

L

L

A

K

Q

N

K

T

H

P

G

D

W

A

F

Y

L

M

A

L

R

Q

Q

Q

F

F

E

D

N

N

E

P

A

A

N

N

P

P

A

K

Y

I

H

H

R

Y

N

E

T

T

G

G

P

P

E

E

I

I

L

W

S

D

D

D

F

T

A

K

G

G

R

-

R

K

L

V

D

D

F

I

W

F

V

V

S

A

G

G

W
x
I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

T

T

G

G

A

V

G

G

E

R

I

F

I

I

K

K

K

E

A

K

R

N

P

P

Q

K

T

T

R

Q

I

V

I

I

A

G

A

V

E

E

P

P

A

T

N

E

A

S

S

D

L

I

L

L

T

S

G

G

A

G

E

K

F

P

K

G

S

P

H

H

K

K

V

I

Q

Q

G
|
G

W

I

T

G

P

A

D

G

F

F

V

I

P

P

A

K

V

N

L

L

N

D

R

Q

S

K

V

I

C

M

D

D

Q

E

I

V

V

I

T

A

V

I

T

S

D

S

D

E

A

E

A

A

I

I

Q

E

Y

T

S

A

R

K

E

Q

L

L

A

A

S

L

K

K

E

E

G

G

I

L

F

M

C

V

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

F

A

A

A

L

I

Q

K

V

V

A

A

Q

K

G

-

A

R

P

P

Q

E

-

N

-

A

G

G

S

K

I

L

I

I

L

A

A

V

M

V

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

E

Q

G

S

I

I

N

R

E

E

active site: K54 (= K42), S277 (= S264)
binding pyridoxal-5'-phosphate: K54 (= K42), N85 (= N72), I188 (≠ W175), G189 (= G176), T190 (= T177), G191 (= G178), G192 (= G179), T193 (= T180), G233 (= G220), S277 (= S264), P304 (= P290)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
44% identity, 96% coverage: 1:303/314 of query aligns to 2:309/310 of 4lmbA

query
sites
4lmbA

M

L

I

I

Y

A

D

R

N

D

I

I

L

T

G

Q

T

L

I

V

G

G

R

R

T

T

P

P

I

L

V

V

K

Q

I

L

N

N

R

R

L

I

A

P

P

V

A

A

H

E

-

G

-

V

-

K

V

A

E

R

M

I

Y

V

V

V

K

K

C

L

E

E

F

S

F

M

N

N

P

P

L

A

A

A

S

S

V

V

K
|
K

D

D

R

R

L

I

A

G

I

V

A

S

I

M

I

V

E

E

D

D

A

A

E

E

Q

A

R

A

G

G

V

L

L

I

K

H

P

P

G

D

Q

K

T

T

V

I

-

L

V

V

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

C

A

A

A

K

K

G

G

Y

Y

P

R

F

L

V

V

A

L

V

T

M

M

A

P

E

E

S

T

F
x
M

S

S

V

L

E

E

R

R

K

A

V

M

M

L

R

K

M

A

L

Y

G

G

A

A

K

Q

V

L

V

E

L

L

T

T

P

P

A

G

A

S

G

Q

R

G

A
x
M

S

E

G

G

M

A

V

I

V

T

K

R

A

A

A

E

E

E

L

I

A

V

Q

E

K

N

H

T

-

P

G

N

W

A

F

Y

L

S

A

L

R

Q

Q
|
Q

F
|
F

E

R

N

N

E

P

A

A

N

N

P

P

A

K

Y

I

H

H

R

R

N

E

T

T

G

A

P

E

E

E

I

I

L

W

S

A

D

D

F

T

A

D

G

G

R

-

R

L

L

V

D

D

F

I

W

V

V

I

S

G

G

G

W
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

A

I

G

A

E

E

I

T

I

I

K

K

K

P

A

R

R

R

P

P

Q

Q

T

F

R

Q

I

A

I

I

A

A

A

V

E

E

P

P

A

S

N

N

A

S

S

P

L

V

L

L

T

S

G

G

A

G

E

Q

F

P

K

G

S

P

H

H

K

K

V

I

Q
|
Q

G
|
G

W
x
I

T

G

P
x
A

D

G

F

F

V

I

P

P

A

A

V

I

L

F

N

R

R

P

S

E

V

L

C

I

D

D

Q

E

I

V

V

I

T

I

V

V

T

D

D

D

D

T

A

E

A

A

I

F

Q

A

Y

Y

S

A

R

R

E

R

L

L

A

A

S

R

K

Q

E

E

G

G

I

L

F

L

C

S

G

G

I

I

S
|
S

S

A

G

G

A

A

T

A

F

L

A

W

A

A

L

I

Q

Q

V

V

A

G

Q

K

G

R

A

P

P

E

Q

N

G

E

S

D

I

K

I

L

L

I

A

V

M

M

L

I

-

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

E

K

active site: K46 (= K42), S269 (= S264)
binding cysteine: K46 (= K42), T74 (= T69), S75 (= S70), N77 (= N72), T78 (= T73), M101 (≠ F96), M125 (≠ A120), M125 (≠ A120), Q147 (= Q141), F148 (= F142), Q224 (= Q219), G225 (= G220), G225 (= G220), I226 (≠ W221), A228 (≠ P223)
binding pyridoxal-5'-phosphate: K46 (= K42), N77 (= N72), V180 (≠ W175), G181 (= G176), T182 (= T177), G183 (= G178), T185 (= T180), G225 (= G220), S269 (= S264), P296 (= P290)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 98% coverage: 2:310/314 of query aligns to 75:386/392 of P47999

query
sites
P47999

I

I

Y

A

D

D

N

N

I

A

L

A

G

Q

T

L

I

I

G

G

R

K

T

T

P

P

I

M

V

V

K

Y

I

L

N

N

R

N

L

V

A

V

P

K

A

G

H

C

V

V

-

A

E

S

M

V

Y

A

V

A

K

K

C

L

E

E

F

I

F

M

N

E

P

P

L

C

A

C

S

S

V

V

K

K

D

D

R

R

L

I

A

G

I

Y

A

S

I

M

I

I

E

T

D

D

A

A

E

E

Q

E

R

K

G

G

V

L

L

I

K

T

P

P

G

G

Q

K

T

S

V

V

-

L

V

V

E

E

A

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

C

A

A

A

A

S

K

K

G

G

Y

Y

P

K

F

L

V

I

A

L

V

T

M

M

A

P

E

A

S

S

F

M

S

S

V

L

E

E

R

R

K

V

V

L

M

L

R

R

M

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

E

A

P

A

A

G

K

R

G

A

M

S

T

G

G

M

A

V

I

V

Q

K

K

A

A

A

E

E

E

L

I

A

L

Q

K

K

K

H

T

-

P

G

N

W

S

F

Y

L

M

A

L

R

Q

Q

Q

F

F

E

D

N

N

E

P

A

A

N

N

P

P

A

K

Y

I

H

H

R

Y

N

E

T

T

G

G

P

P

E

E

I

I

L

W

S

E

D

D

F

T

A

R

G

G

R

-

R

K

L

I

D

D

F

I

W

L

V

V

S

A

G

G

W

I

G

G

T

T

G

G

T

T

L

I

T

T

G

G

A

V

G

G

E

R

I

F

I

I

K

K

K

E

A

R

R

K

P

P

Q

E

T

L

R

K

I

V

I

I

A

G

A

V

E

E

P

P

A

T

N

E

A

S

S

A

L

I

L

L

T

S

G

G

A

G

E

K

F

P

K

G

S

P

H

H

K

K

V

I

Q

Q

G

G

W

I

T

G

P

A

D

G

F

F

V

V

P

P

A

K

V

N

L

L

N

D

R

L

S

A

V

I

C

V

D

D

Q

E

I

Y

V

I

T

A

V

I

T

S

D

S

D

E

A

E

A

A

I

I

Q

E

Y

T

S

S

R

K

E

Q

L

L

A

A

S

L

K

Q

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S

S

G

G

A

A

T

A

F

A

A

A

L

I

Q

Q

V

V

A

A

Q

K

G

R

A

P

P

E

Q

N

-

A

G

G

S

K

I

L

I

I

L

A

A

V

M

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

F

F

E

Q

G

S

I

I

N

R

E

E

A

E

S

C

D

E

Sites not aligning to the query:

61 modified: N-acetylalanine

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
41% identity, 94% coverage: 9:302/314 of query aligns to 7:292/294 of 2bhtA

query
sites
2bhtA

T

T

I

I

G

G

R

N

T

T

P

P

I

L

V

V

K

K

I

L

N

Q

R

R

L

M

A

G

P

P

A

D

H

N

-

G

V

S

E

E

M

V

Y

W

V

L

K

K

C

L

E

E

F

G

F

N

N

N

P

P

L

A

A

G

S

S

V

V

K
|
K

D

D

R

R

L

A

A

A

I

L

A

S

I

M

I

I

E

V

D

E

A

A

E

E

Q

K

R

R

G

G

V

R

L

I

K

K

P

P

G

G

Q

D

T

V

V

L

V

I

E

E

A

A

T

T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

I

C

A

A

A

A

L

K

K

G

G

Y

Y

P

R

F

M

V

K

A

L

V

L

M

M

A

P

E

D

S

N

F

M

S

S

V

Q

E

E

R

R

K

A

V

A

M

M

R

R

M

A

L

Y

G

G

A

A

K

E

V

L

V

I

L

L

T

V

P

T

A

K

A

E

G

Q

R

G

A

M

S

E

G

G

M

A

V

R

V

D

K

L

A

A

A

L

E

E

L

M

A

A

Q

N

K

R

H

G

G

E

W

G

F

K

L

L

A

L

R

D

Q

Q

F

F

E

N

N

N

E

P

A

D

N

N

P

P

A

K

Y

A

H

H

R

Y

N

T

T

T

G

G

P

P

E

E

I

I

L

W

S

Q

D

Q

F

T

A

G

G

G

R

-

R

R

L

I

D

T

F

H

W

F

V

V

S

S

G

S

W
x
M

G
|
G

T
|
T

G
x
T

G

G

T
|
T

L

I

T

T

G

G

A

V

G

S

E

E

I

F

I

M

K

R

K

E

A

Q

R

S

P

K

Q

P

T

V

R

T

I

I

I

V

A

G

A

L

E
x
Q

P

P

A

E

N

E

A
x
G

S

S

L

S

L

I

T

P

G
|
G

A

-

E

-

F

-

K

-

S

-

H

-

K

-

V

I

Q

R

G

R

W

W

T

P

P

T

D

E

F

Y

V

L

P

P

A

G

V

I

L

F

N

N

R

A

S

S

V

L

C

V

D

D

Q

E

I

V

V

L

T

D

V

I

T

H

D

Q

D

R

A

D

A

A

I

E

Q

N

Y

T

S

M

R

R

E

E

L

L

A

A

S

V

K

R

E

E

G

G

I

I

F

F

C

C

G

G

I

V

S
|
S

S

S

G

G

A

G

T

A

F

V

A

A

A

G

A

A

L

L

Q

R

V

V

A

A

Q

K

G

A

A

N

P

P

Q

D

G

-

S

A

I

V

L

V

A

A

M

I

L

I

P
x
C

D

D

T

R

G

G

E

D

R

R

Y

Y

L

L

S

S

T

T

P

G

L

V

F

F

active site: K41 (= K42), S69 (= S70), Q199 (≠ E201), G203 (≠ A205), S255 (= S264), C280 (≠ P290)
binding pyridoxal-5'-phosphate: K41 (= K42), N71 (= N72), M173 (≠ W175), G174 (= G176), T175 (= T177), T176 (≠ G178), T178 (= T180), G208 (= G210), S255 (= S264), C280 (≠ P290)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
46% identity, 96% coverage: 2:302/314 of query aligns to 4:312/323 of P0A1E3

query
sites
P0A1E3

I

I

Y

Y

D

E

N

D

I

N

L

S

G

L

T

T

I

I

G

G

R

H

T

T

P

P

I

L

V

V

K

R

I

L

N

N

R

R

L

I

A

G

P

N

A

G

H

R

V

I

E

-

M

-

Y

L

V

A

K

K

C

V

E

E

F

S

F

R

N

N

P

P

L

S

A

F

S

S

V

V

K

K

D

C

R

R

L

I

A

G

I

A

A

N

I

M

I

I

E

W

D

D

A

A

E

E

Q

K

R

R

G

G

V

V

L

L

K

K

P

P

G

G

Q

V

T

E

V

L

V

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

Y

V

V

C

A

A

A

K

R

G

G

Y

Y

P

K

F

L

V

T

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S

V

I

E

E

R

R

K

K

V

L

M

L

R

K

M

A

L

L

G

G

A

A

K

N

V

L

V

V

L

L

T

T

P

E

A

G

A

A

G

K

R

G

A

M

S

K

G

G

M

A

V

I

V

Q

K

K

A

A

A

E

E

E

L

I

-

V

-

A

A

S

Q

D

K

P

H

Q

G

K

W

Y

F

L

L

L

A

L

R

Q

Q

Q

F

F

E

S

N

N

E

P

A

A

N

N

P

P

A

E

Y

I

H

H

R

E

N

K

T

T

G

G

P

P

E

E

I

I

L

W

S

E

D

D

F

T

A

D

G

G

R

-

R

Q

L

V

D

D

F

V

W

F

V

I

S

S

G

G

W

V

G

G

T

T

G

G

T

T

L

L

T

T

G

G

A

V

G

T

E

R

I

Y

I

I

K

K

K

G

A

T

R

K

P

G

Q

K

T

T

R

D

I

L

I

I

-

T

-

V

A

A

A

V

E

E

P

P

A

T

N

D

-

S

-

P

-

V

-

I

A

A

S

Q

L

A

L

L

T

A

G

G

A

E

E

E

F

I

K

K

-

P

-

G

S

P

H

H

K

K

V

I

Q

Q

G

G

W

I

T

G

P

A

D

G

F

F

V

I

P

P

A

G

V

N

L

L

N

D

R

L

S

K

V

L

C

I

D

D

Q

K

I

V

V

V

T

G

V

I

T

T

D

N

D

E

A

E

A

A

I

I

Q

S

Y

T

S

A

R

R

E

R

L

L

A

M

S

E

K

E

E

E

G

G

I

I

F

L

C

A

G

G

I

I

S
|
S

S

S

G

G

A

A

T

A

F

V

A

A

L

L

Q

K

V

L

A

Q

Q

E

G

D

A

E

P

S

-

F

Q

T

G

N

S

K

I

N

I

I

L

V

A

V

M

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

N72 (= N72) binding pyridoxal 5'-phosphate
S273 (= S264) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
46% identity, 96% coverage: 2:302/314 of query aligns to 5:313/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

Y

Y

D

E

N

D

I

N

L

S

G

L

T

T

I

I

G

G

R

H

T

T

P

P

I

L

V

V

K

R

I

L

N

N

R

R

L

I

A

G

P

N

A

G

H

R

V

I

E

-

M

-

Y

L

V

A

K

K

C

V

E

E

F

S

F

R

N

N

P

P

L

S

A

F

S

S

V

V

K
|
K

D

C

R

R

L

I

A

G

I

A

A

N

I

M

I

I

E

W

D

D

A

A

E

E

Q

K

R

R

G

G

V

V

L

L

K

K

P

P

G

G

Q

V

T

E

V

L

V

V

E

E

A

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

Y

V

V

C

A

A

A

K

R

G

G

Y

Y

P

K

F

L

V

T

A

L

V

T

M

M

A

P

E

E

S

T

F

M

S

S