SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_066921978.1 NCBI__GCF_001579945.1:WP_066921978.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
27% identity, 85% coverage: 14:272/304 of query aligns to 12:266/284 of Q58761

query
sites
Q58761

L

L

A

A

G

F

T

K

I

V

A

A

Q

K

P

L

L

L

Q

N

A

T

R

K

I

L

S

T

P

R

V

V

A

E

W

Y

R

K

H

R

F

F

P

P

D
|
D

G
x
N

E
|
E

S

I

L

Y

V

V

T

R

L

I

D

V

D

D

T

E

L

I

A

N

G

D

A

D

D

E

V

A

A

V

I

I

L

I

A

N

S

T

L

Q

R

K

S

N

A

Q

D

N

A

D

L

A

A

I

L

V

P

E

L

T

R

I

F

L

A

L

A

C

L

D

T

A

A

L

R

R

E

D

F

E

G

G

A

V

R

K

S

K

V

I

G

T

L

L

I

V

A

A

P

P

Y

Y

L

L

G

A

Y

Y

M

A

R

R

Q

Q

D

D

T

K

R

K

F

F

H

N

R

P

G

G

E

E

A

A

V

I

S

S

T

I

P

R

L

A

F

L

A

A

Q

K

Y

I

L

Y

E

S

E

N

A

I

F

V

D

D

W

K

L

L

V

I

T

T

V

I

D

N

P

P

H

H

L

E

H

T

R

H

V

I

E

-

R

-

L

-

Q

K

S

D

L

F

Y

F

R

T

I

I

P

P

T

F

G

I

H

Y

V

G

S

D

A

A

T

V

P

P

A

K

V

L

A

A

D

E

W

Y

I

V

A

K

K

D

T

K

V

L

P

N

D

D

A

P

I

I

L

V

I

L

G

A

P

P

D

D

S

K

E

G

S

A

E

L

Q

E

W

F

V

A

A

K

G

T

I

A

A

S

A

K

R

I

S

L

G

N

M

A

P

E

Y

Y

Q

D

V

Y

L

L

A

E

K

K

E

T

R

R

H

L

G

S

D

P

Y

T

D

E

V

I

R

Q

V

I

S

A

L

-

P

P

D

K

P

T

A

L

A

D

V

A

A

K

G

D

R

R

T

D

P

V

V

F

L

I

I

V

D
|
D

D

D

I

I

V

I

S
|
S

S
x
T

G
|
G

H
x
G

T
|
T

I

M

L

A

E

T

T

A

L

V

A

K

H

L

L

L

R

K

Q

E

L

Q

S

G

L

A

P

K

A

K

P

I

M

I

L

A

V

A

A

C

I

V

H

H

P

P

V

V

F

L

A

I

G

G

D

D

A

A

Y

L

A

N

R

K

L

L

Q

Y

E

S

A

A

G

G

L

V

A

E

R

E

I

V

V

V

S

G

T

T

D

D

T

T

DNE 34:36 (≠ DGE 36:38) binding ATP
D212 (= D218) binding D-ribose 5-phosphate
STGGT 216:220 (≠ SSGHT 222:226) binding D-ribose 5-phosphate

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
27% identity, 85% coverage: 14:272/304 of query aligns to 12:256/274 of 1u9zA

query
sites
1u9zA

L

L

A

A

G

F

T

K

I

V

A

A

Q

K

P

L

L

L

Q

N

A

T

R

K

I

L

S

T

P

R

V

V

A

E

W

Y

R

K

H

R

F
|
F

P

P

D
|
D

G

N

E
|
E

S

I

L

Y

V

V

T

R

L

I

D

V

D

D

T

E

L

I

A

N

G

D

A

D

D

E

V

A

A

V

I

I

L

I

A

N

S

T

L

Q

R

K

S

N

A

Q

D

N

A

D

L

A

A

I

L

V

P

E

L

T

R

I

F

L

A

L

A

C

L

D

T

A

A

L

R

R

E

D

F

E

G

G

A

V

R

K

S

K

V

I

G

T

L

L

I

V

A

A

P

P

Y

Y

L

L

G

A

Y

Y

M

A

R
|
R

Q
|
Q

D

D

T

K

R

K

F
|
F

H

N

R

P

G

G

E

E

A

A

V

I

S

S

T

I

P

R

L

A

F

L

A

A

Q

K

Y

I

L

Y

E

S

E

N

A

I

F

V

D

D

W

K

L

L

V

I

T

T

V

I

D

N

P

P

H
|
H

L

E

H

T

R

H

V

I

E

-

R

-

L

-

Q

K

S

D

L

F

Y

F

R

T

I

I

P

P

T

F

G

I

H

Y

V

G

S

D

A

A

T

V

P

P

A

K

V

L

A

A

D

E

W

Y

I

V

A

K

K

D

T

K

V

L

P

N

D

D

A

P

I

I

L

V

I

L

G

A

P

P

D
|
D

S

K

E

G

S

A

E

L

Q

E

W

F

V

-

A

-

G

-

I

-

A

-

A

A

R

K

S

T

G

A

M

-

P

-

Y

-

Q

-

V

-

L

-

A

S

K

K

E

I

R

L

H

N

G

A

D

E

Y

Y

D

D

V

Y

R

L

V

E

S

I

L

A

P

P

D

K

P

T

A

L

A

D

V

A

A

K

G

D

R

R

T

D

P

V

V

F

L

I

I

V

D
|
D

D

D

I

I

V
x
I

S
|
S

S
x
T

G
|
G

H

G

T
|
T

I

M

L

A

E

T

T

A

L

V

A

K

H

L

L

L

R

K

Q

E

L

Q

S

G

L

A

P

K

A

K

P

I

M

I

L

A

V

A

A

C

I

V

H

H

P

P

V

V

F

L

A

I

G

G

D

D

A

A

Y

L

A

N

R

K

L

L

Q

Y

E

S

A

A

G

G

L

V

A

E

R

E

I

V

V

V

S

G

T

T

D

D

T

T

binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R92 (= R94), Q93 (= Q95), F97 (= F99), H125 (= H127)
binding ribose-5-phosphate: D163 (= D168), D202 (= D218), I205 (≠ V221), S206 (= S222), T207 (≠ S223), G208 (= G224), T210 (= T226)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
30% identity, 87% coverage: 11:273/304 of query aligns to 13:271/291 of Q97Z86

query
sites
Q97Z86

D

D

A

E

V

S

L

L

A

S

G

K

T

I

I

L

A

S

Q

I

P

P

L

L

Q

-

A

-

R

-

I

-

S

V

P

K

V

V

A

E

W

N

R

K

H

I

F

F

P

P

D

D

G

G

E

E

S

S

L

Y

V

I

T

R

L

V

D

P

D

S

T

S

L

I

A

R

G

D

A

E

D

E

V

V

A

L

I

L

L

V

A

Q

S

T

L

T

R

D

S

Y

-

P

A

Q

D

D

A

K

L

H

A

L

L

I

P

E

L

L

R

F

F

L

A

I

A

A

L

E

T

T

A

I

R

R

E

D

F

L

G

G

A

A

R

K

S

K

V

L

G

T

L

A

I

I

A

V

P

P

Y

Y

L

L

G

A

Y

Y

M

S

R

R

Q

Q

D

D

T

R

R

R

F

F

H

K

R

D

G

G

E

E

A

A

V

I

S

S

T

I

P

K

L

T

F

I

A

L

Q

H

Y

I

L

L

E

S

E

E

-

V

A

G

F

V

D

N

W

T

L

L

V

V

T

V

V

V

D

E

P

P

H

H

L

-

H

-

R

K

V

P

E

E

R

E

L

L

Q

-

S

S

L

Y

Y

F

R

K

I

G

P

E

T

L

G

K

H

I

V

V

S

H

A

P

T

Y

P

H

A

Q

V

I

A

A

D

R

W

K

I

I

A

K

K

E

T

I

V

I

P

E

D

D

A

P

I

F

L

I

I

L

G

A

P

P

D

D

S

R

E

G

S

A

E

L

Q

D

W

R

V

A

A

R

G

K

I

I

A

A

A

E

R

E

S

I

G

N

M

A

P

P

Y

Y

Q

S

V

Y

L

I

A

E

K

K

E

E

R

R

-

D

H

R

G

T

D

T

Y

G

D

E

V

V

R

R

V

I

S

K

L

E

P

A

D

P

P

N

A

I

A

N

V

L

A

K

G

G

R

K

T

D

P

V

V

V

L

I

I

I

D

D

I

I

V

I

S
|
S

S
x
T

G
|
G

H
x
G

T
|
T

I

I

L

V

E

Q

T

A

L

T

A

R

H

L

L

A

R

Y

Q

S

L

L

S

G

L

A

P

K

A

S

P

V

M

T

L

A

V

A

A

A

I

I

H

H

P

L

V

L

F

L

A

V

G

G

D

G

A

A

Y

K

A

E

R

R

L

L

Q

R

E

E

A

V

G

G

L

V

A

K

R

T

I

L

V

I

S

G

T

T

D

N

T

T

I

I

STGGT 220:224 (≠ SSGHT 222:226) binding D-ribose 5-phosphate

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
28% identity, 91% coverage: 5:282/304 of query aligns to 5:275/287 of 3mbiA

query
sites
3mbiA

L

I

M

I

P

A

F

L

P

R

S

S

D

S

A

L

V

K

L

L

A

A

G

A

T

R

I

I

A

A

Q

E

P

E

L

L

Q

K

A

T

R

E

I

-

S

-

P

P

V

V

A

M

-

P

-

D

W

E

R

R

H

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

L

Y

V

L

T

R

L

Y

D

D

D

E

T

D

L

L

A

T

G

G

A

H

D

N

V

I

A

F

I

I

L

I

A

G

S

N

L

T

R

H

S

S

-

D

A

A

D

E

A

V

L

M

A

E

L

M

P

I

L

L

R

T

F

L

A

S

A

A

L

I

T

-

A

-

R

Q

E

D

F

Y

G

R

A

T

R

K

S

S

V

V

G

N

L

I

I

I

A

A

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

T

Q

R

R

F
x
Y

H

K

R

N

G

G

E

E

A

P

V

I

S

S

T

S

P

Q

L

I

F

L

A

T

Q

E

Y

I

L

Y

E

S

A

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

V

D

E

E

R

K

L

T

Q

L

S

S

L

Y

Y

S

R

K

I

V

P

K

T

F

G

S

H

D

V

L

S

H

A

A

T

N

P

D

A

A

V

I

A

V

D

R

W

Y

I

Y

A

-

K

K

T

N

V

V

P

D

D

V

A

D

I

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

A

A

G

D

I

I

A

S

A

A

R

K

S

L

G

G

M

K

P

K

Y

H

Q

F

V

F

L

I

A

E

K
|
K

E

K

R
|
R

H

I

G

D

D

D

Y

R

D

T

V

V

R

E

V

M

S

K

L

V

P

P

D

N

P

-

A

V

A

D

V

V

A

N

G

G

R

K

T

K

P

L

V

L

L

I

I

V

D

D

I

I

V

I

S
|
S

S
x
T

G
|
G

H

G

T
|
T

I

I

L

A

E

K

T

S

L

S

A

G

H

L

L

L

R

R

Q

E

L

K

S

G

L

A

P

S

A

K

P

I

M

Y

L

V

V

S

A

A

I

V

H

H

P

G

V

L

F

F

A

V

G

N

D

G

A

S

Y

E

A

N

R

K

-

I

L

L

Q

Q

E

N

A

A

G

D

L

-

A

-

R

E

I

I

V

H

S

V

T

T

D

D

T

T

I

V

A

E

H

S

P

K

S

F

N

S

A

D

I

I

A

S

L

V

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R93 (= R94), Q94 (= Q95), Y98 (≠ F99), H126 (= H127), K186 (= K191), R188 (= R193)
binding magnesium ion: H95 (≠ D96), H126 (= H127)
binding phosphate ion: R188 (= R193), S216 (= S222), T217 (≠ S223), G218 (= G224), T220 (= T226)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
28% identity, 91% coverage: 5:282/304 of query aligns to 3:273/286 of Q97CA5

query
sites
Q97CA5

L

I

M

I

P

A

F

L

P

R

S

S

D

S

A

L

V

K

L

L

A

A

G

A

T

R

I

I

A

A

Q

E

P

E

L

L

Q

K

A

T

R

E

I

-

S

-

P

P

V

V

A

M

-

P

-

D

W

E

R

R

H

R

F

F

P

P

D
|
D

G
|
G

E
|
E

S

L

L

Y

V

L

T

R

L

Y

D

D

D

E

T

D

L

L

A

T

G

G

A

H

D

N

V

I

A

F

I

I

L

I

A

G

S

N

L

T

R

H

S

S

-

D

A

A

D

E

A

V

L

M

A

E

L

M

P

I

L

L

R

T

F

L

A

S

A

A

L

I

T

-

A

-

R

Q

E

D

F

Y

G

R

A

T

R

K

S

S

V

V

G

N

L

I

I

I

A

A

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

T

Q

R

R

F

Y

H

K

R

N

G

G

E

E

A

P

V

I

S

S

T

S

P

Q

L

I

F

L

A

T

Q

E

Y

I

L

Y

E

S

A

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H

H

L

-

H

-

R

-

V

D

E

E

R

K

L

T

Q

L

S

S

L

Y

Y

S

R

K

I

V

P

K

T

F

G

S

H

D

V

L

S

H

A

A

T

N

P

D

A

A

V

I

A

V

D

R

W

Y

I

Y

A

-

K

K

T

N

V

V

P

D

D

V

A

D

I

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

A

A

G

D

I

I

A

S

A

A

R

K

S

L

G

G

M

K

P

K

Y

H

Q

F

V

F

L

I

A

E

K

K

E

K

R
|
R

H

I

G

D

D

D

Y

R

D

T

V

V

R

E

V

M

S

K

L

V

P

P

D

N

P

-

A

V

A

D

V

V

A

N

G

G

R

K

T

K

P

L

V

L

L

I

I

V

D
|
D

D

D

I

I

V

I

S
|
S

S
x
T

G
|
G

H
x
G

T
|
T

I

I

L

A

E

K

T

S

L

S

A

G

H

L

L

L

R

R

Q

E

L

K

S

G

L

A

P

S

A

K

P

I

M

Y

L

V

V

S

A

A

I

V

H

H

P

G

V

L

F

F

A

V

G

N

D

G

A

S

Y

E

A

N

R

K

-

I

L

L

Q

Q

E

N

A

A

G

D

L

-

A

-

R

E

I

I

V

H

S

V

T

T

D

D

T

T

I

V

A

E

H

S

P

K

S

F

N

S

A

D

I

I

A

S

L

V

DGE 34:36 (= DGE 36:38) binding ATP
RQH 91:93 (≠ RQD 94:96) binding ATP
R186 (= R193) binding D-ribose 5-phosphate
D210 (= D218) binding D-ribose 5-phosphate
STGGT 214:218 (≠ SSGHT 222:226) binding D-ribose 5-phosphate

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
28% identity, 93% coverage: 5:287/304 of query aligns to 3:278/284 of 3lpnA

query
sites
3lpnA

L

I

M

I

P

A

F

L

P
x
R

S

S

D

S

A

L

V
x
K

L

L

A

A

G

A

T
x
R

I

I

A

A

Q

E

P

E

L

L

Q

K

A

T

R

E

I

-

S

-

P

P

V

V

A

M

-

P

-

D

W

E

R

R

H

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

L

Y

V

L

T

R

L

Y

D

D

D

E

T

D

L

L

A

T

G

G

A

H

D

N

V

I

A

F

I

I

L

I

A

G

S

N

L

T

R

H

S
|
S

-

D

A

A

D

E

A

V

L

M

A

E

L

M

P

I

L

L

R

T

F

L

A

S

A

A

L

I

T

-

A

-

R

Q

E

D

F

Y

G

R

A

T

R

K

S

S

V

V

G

N

L

I

I

I

A

A

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

T

Q

R

R

F
x
Y

H

K

R

N

G

G

E

E

A

P

V

I

S

S

T

S

P

Q

L

I

F

L

A

T

Q

E

Y

I

L

Y

E

S

A

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

V
x
D

E

E

R

K

L

T

Q

L

S

S

L

Y

Y

S

R

K

I

V

P

K

T

F

G

S

H

D

V

L

S

H

A

A

T

N

P

D

A

A

V

I

A

V

D

R

W

Y

I

-

A

Y

K

K

T

N

V

V

P

D

D

V

A

D

I

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

A

A

G

D

I

I

A

S

A

A

R

K

S

L

G

G

M

K

P

K

Y

H

Q

F

V

F

L

I

A

E

K

K

E

K

R

R

H

I

G

D

D

D

Y

R

D

T

V

V

R

E

V

M

S

K

L

V

P

P

D

N

P

-

A

V

A

D

V

V

A

N

G

G

R

K

T

K

P

L

V

L

L

I

I

V

D

D

I

I

V

I

S
|
S

S
x
T

G
|
G

H

G

T
|
T

I

I

L

A

E

K

T

S

L

S

A

G

H

L

L

L

R

R

Q

E

L

K

S

G

L

A

P

S

A

K

P

I

M

Y

L

V

V

S

A

A

I

V

H

H

P

G

V

L

F

F

A

V

G

N

D

G

A

S

Y

E

A

N

R

K

-

I

L

L

Q

Q

E

N

A

A

G

D

L

-

A

-

R

E

I

I

V

H

S

V

T

T

D

D

T

T

I

V

A

E

H

S

P

K

S

F

N

S

A

D

I

I

A

S

L

V

G

Y

A

Q

D

E

L

V

A

C

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), H93 (≠ D96), Y96 (≠ F99), H124 (= H127), D125 (≠ V131)
binding sulfate ion: R7 (≠ P9), K11 (≠ V13), R15 (≠ T17), S58 (= S60), S214 (= S222), T215 (≠ S223), G216 (= G224), T218 (= T226)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
28% identity, 91% coverage: 5:282/304 of query aligns to 3:273/285 of 3mbiD

query
sites
3mbiD

L

I

M

I

P

A

F

L

P

R

S

S

D

S

A

L

V

K

L

L

A

A

G

A

T

R

I

I

A

A

Q

E

P

E

L

L

Q

K

A

T

R

E

I

-

S

-

P

P

V

V

A

M

-

P

-

D

W

E

R

R

H

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

L

Y

V

L

T

R

L

Y

D

D

D

E

T

D

L

L

A

T

G

G

A

H

D

N

V

I

A

F

I

I

L

I

A

G

S

N

L

T

R

H

S

S

-

D

A

A

D

E

A

V

L

M

A

E

L

M

P

I

L

L

R

T

F

L

A

S

A

A

L

I

T

-

A

-

R

Q

E

D

F

Y

G

R

A

T

R

K

S

S

V

V

G

N

L

I

I

I

A

A

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

T

Q

R

R

F
x
Y

H

K

R

N

G

G

E

E

A

P

V

I

S

S

T

S

P

Q

L

I

F

L

A

T

Q

E

Y

I

L

Y

E

S

A

Y

F

S

D

N

W

S

L

I

V

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

V

D

E

E

R

K

L

T

Q

L

S

S

L

Y

Y

S

R

K

I

V

P

K

T

F

G

S

H

D

V

L

S

H

A

A

T

N

P

D

A

A

V

I

A

V

D

R

W

Y

I

-

A

Y

K

K

T

N

V

V

P

D

D

V

A

D

I

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

A

A

G

D

I

I

A

S

A

A

R

K

S

L

G

G

M

K

P

K

Y

H

Q

F

V

F

L

I

A

E

K
|
K

E

K

R
|
R

H

I

G

D

D

D

Y

R

D

T

V

V

R

E

V

M

S

K

L

V

P

P

D

N

P

-

A

V

A

D

V

V

A

N

G

G

R

K

T

K

P

L

V

L

L

I

I

V

D
|
D

I
|
I

V

I

S
|
S

S
x
T

G
|
G

H

G

T
|
T

I

I

L

A

E

K

T

S

L

S

A

G

H

L

L

L

R

R

Q

E

L

K

S

G

L

A

P

S

A

K

P

I

M

Y

L

V

V

S

A

A

I

V

H

H

P

G

V

L

F

F

A

V

G

N

D

G

A

S

Y

E

A

N

R

K

-

I

L

L

Q

Q

E

N

A

A

G

D

L

-

A

-

R

E

I

I

V

H

S

V

T

T

D

D

T

T

I

V

A

E

H

S

P

K

S

F

N

S

A

D

I

I

A

S

L

V

binding adenosine-5'-diphosphate: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), Y96 (≠ F99), H124 (= H127), K184 (= K191), R186 (= R193)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R94), H124 (= H127), K184 (= K191), R186 (= R193), D210 (= D218), D211 (= D219), I212 (= I220), S214 (= S222), T215 (≠ S223), G216 (= G224), T218 (= T226)

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
29% identity, 85% coverage: 17:273/304 of query aligns to 15:258/278 of 4twbA

query
sites
4twbA

T

S

I

L

A

S

Q

K

P

I

L

L

Q

S

A

I

R

P

I

L

S

V

P

K

V

V

A

E

W

N

R

K

H

I

F
|
F

P

P

D
|
D

G

G

E
|
E

S

S

L

Y

V

I

T

R

L

V

D

P

D

S

T

S

L

I

A

R

G

D

A

E

D

E

V

V

A

L

I

L

L

V

A

Q

S

T

L

T

R

D

S

Y

-

P

A

Q

D

D

A

K

L

H

A

L

L

I

P

E

L

L

R

F

F

L

A

I

A

A

L

E

T

T

A

I

R

R

E

D

F

L

G

G

A

A

R

K

S

K

V

L

G

T

L

A

I

I

A

V

P

P

Y

Y

L

L

G

A

Y

Y

M

S

R
|
R

Q

Q

D

D

T

R

R

R

F
|
F

H

K

R

D

G

G

E

E

A

A

V

I

S

S

T

I

P

K

L

T

F

I

A

L

Q

H

Y

I

L

L

E

S

E

E

-

V

A

G

F

V

D

N

W

T

L

L

V

V

T

V

V

V

D

E

P

P

H
|
H

L

-

H

-

R

K

V

P

E

E

R

E

L

L

Q

-

S

S

L

Y

Y

F

R

K

I

G

P

E

T

L

G

K

H

I

V

V

S

H

A

P

T

Y

P

H

A

Q

V

I

A

A

D

R

W

K

I

I

A

K

K

E

T

I

V

I

P

E

D

D

A

P

I

F

L

I

I

L

G

A

P

P

D

D

S

R

E

G

S

A

E

L

Q

D

W

R

V

A

A

R

G

K

I

I

A

A

A

E

R

E

S

I

G

N

M

A

P

P

Y

Y

Q

S

V

Y

L

I

A

E

K

K

E

E

R

R

H

N

G

-

D

-

Y

-

D

-

V

-

R

-

V

-

S

-

L

-

P

-

D

-

P

-

A

I

A

N

V

L

A

K

G

G

R

K

T

D

P

V

V

V

L

I

I

I

D

D

I

I

V

I

S

S

S

T

G

G

H

G

T

T

I

I

L

V

E

Q

T

A

L

T

A

R

H

L

L

A

R

Y

Q

S

L

L

S

G

L

A

P

K

A

S

P

V

M

T

L

A

V

A

A

A

I

I

H

H

P

L

V

L

F

L

A

V

G

G

D

G

A

A

Y

K

A

E

R

R

L

L

Q

R

E

E

A

V

G

G

L

V

A

K

R

T

I

L

V

I

S

G

T

T

D

N

T

T

I

I

binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R93 (= R94), F98 (= F99), H127 (= H127)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
24% identity, 90% coverage: 5:277/304 of query aligns to 12:283/317 of P14193

query
sites
P14193

L

I

M

F

P

S

F

L

P

N

S

S

D

N

A

P

V

E

L

L

A

A

G

K

T

E

I

I

A

A

Q

D

P

I

L

V

Q

G

A

V

R

Q

I

L

S

G

P

K

V

C

A

S

W

V

R

T

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

Q

V

I

T

N

L

I

D

E

T

S

L

I

A

R

G

G

A

C

D

D

V

C

A

Y

I

I

L

I

A

Q

S

S

L

T

R

S

S

D

-

P

A

V

D

N

A

E

L

H

A

I

L

M

P

E

L

L

R

L

F

I

A

M

A

V

L

D

T

A

A

L

R

K

E

R

F

A

G

S

A

A

R

K

S

T

V

I

G

N

L

I

I

V

A

I

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

T

R

R

K

F

A

H

R

R

S

G

R

E

E

A

P

V

I

S

T

T

A

P

K

L

L

F

F

A

A

Q

N

Y

L

L

L

E

E

E

T

A

A

-

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H

H

L

-

H

-

R

-

V

A

E

P

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

I

P

P

T

I

G

D

H

H

V

L

S

M

A

G

T

V

P

P

A

I

V

L

A

G

D

E

W

Y

I

F

-

E

A

G

K

K

T

N

V

L

P

E

D

D

A

I

I

V

L

I

I

V

G

S

P

P

D

D

S

H

E

G

S

G

E

V

Q

T

W

R

V

A

A

R

G

K

I

L

A

A

A

D

R

R

S

L

G

K

M

A

P

P

Y

I

Q

A

V

I

L

I

A

D

K
|
K

E

R

R
|
R

H

P

G
x
R

D

P

Y

-

D
x
N

V

V

R

A

V
x
E

S

V

L

M

P

N

D

I

P

V

A

G

A

N

V

I

A

E

G

G

R

K

T

T

P

A

V

I

L

L

I

I

D

D

I

I

V

I

S
x
D

S
x
T

G
x
A

H
x
G

T
|
T

I

I

L

T

E

L

T

A

L

A

A

N

H

A

L

L

R

V

Q

E

L

N

S

G

L

A

P

K

A

E

P

V

M

Y

L

A

V

C

A

C

I

T

H

H

P

P

V

V

F

L

A

S

G

G

D

P

A

A

Y

V

A

E

R

R

L

I

Q

N

E

N

A

S

G

T

L

I

A

K

R

E

I

L

V

V

S

V

T

T

D

N

T

S

I

I

A

K

H

L

P

P

S

E

RQ 102:103 (= RQ 94:95) binding ATP
K198 (= K191) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R193) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ G195) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ D198) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ V201) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ SSGHT 222:226) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
24% identity, 88% coverage: 5:273/304 of query aligns to 4:262/295 of 1dkuA

query
sites
1dkuA

L

I

M

F

P

S

F

L

P

N

S

S

D

N

A

P

V

E

L

L

A

A

G

K

T

E

I

I

A

A

Q

D

P

I

L

V

Q

G

A

V

R

Q

I

L

S

G

P

K

V

C

A

S

W

V

R

T

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

Q

V

I

T

N

L

I

D

E

T

S

L

I

A

R

G

G

A

C

D

D

V

C

A

Y

I

I

L

I

A

Q

S

S

L

T

R

S

S

D

-

P

A

V

D

N

A

E

L

H

A

I

L

M

P

E

L

L

R

L

F

I

A

M

A

V

L

D

T

A

A

L

R

K

E

R

F

A

G

S

A

A

R

K

S

T

V

I

G

N

L

I

I

V

A

I

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q

Q

D

D

T

R

R
x
K

F

A

H

R

R
x
S

G
x
R

E

E

A

P

V

I

S

T

T

A

P

K

L

L

F

F

A

A

Q

N

Y

L

L

L

E

E

E

T

A

A

-

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H
|
H

L

-

H

-

R

-

V

A

E

P

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

I

P

P

T

I

G
x
D

H

H

V

L

S

M

A

G

T

V

P

P

A

I

V

L

A

G

D

E

W

Y

I

F

-

E

A

G

K

K

T

N

V

L

P

E

D

D

A

I

I

V

L

I

I

V

G

S

P

P

D

D

S

H

E

G

S

G

E

V

Q

T

W

R

V

A

A

R

G

K

I

L

A

A

A

D

R

R

S

L

G

K

M

A

P

P

Y

I

Q

A

V

I

L

I

A

-

K

-

E

-

R

-

H

-

G

-

D

-

Y

-

D

D

V

K

R

R

V

M

S

N

L

I

P

-

D

-

P

V

A

G

A

N

V

I

A

E

G

G

R

K

T

T

P

A

V

I

L

L

I

I

D

D

I

I

V

I

S

D

S

T

G

A

H

G

T

T

I

I

L

T

E

L

T

A

L

A

A

N

H

A

L

L

R

V

Q

E

L

N

S

G

L

A

P

K

A

E

P

V

M

Y

L

A

V

C

A

C

I

T

H

H

P

P

V

V

F

L

A

S

G

G

D

P

A

A

Y

V

A

E

R

R

L

I

Q

N

E

N

A

S

G

T

L

I

A

K

R

E

I

L

V

V

S

V

T

T

D

N

T

S

I

I

binding methyl phosphonic acid adenosine ester: R94 (= R94), H128 (= H127)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R98), S101 (≠ R101), R102 (≠ G102), D141 (≠ G143)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 290, 291, 292, 295

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
24% identity, 90% coverage: 5:277/304 of query aligns to 4:264/297 of 1ibsA

query
sites
1ibsA

L

I

M

F

P

S

F

L

P

N

S

S

D

N

A

P

V

E

L

L

A

A

G

K

T

E

I

I

A

A

Q

D

P

I

L

V

Q

G

A

V

R

Q

I

L

S

G

P

K

V

C

A

S

W

V

R

T

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

Q

V

I

T

N

L

I

D

E

T

S

L

I

A

R

G

G

A

C

D

D

V

C

A

Y

I

I

L

I

A

Q

S

S

L

T

R

S

S

D

-

P

A

V

D

N

A

E

L

H

A

I

L

M

P

E

L

L

R

L

F

I

A

M

A

V

L

D

T

A

A

L

R

K

E

R

F

A

G

S

A

A

R

K

S

T

V

I

G

N

L

I

I

V

A

I

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

T
x
R

R

K

F

S

H

R

R

-

G

-

E

E

A

P

V

I

S

T

T

A

P

K

L

L

F

F

A

A

Q

N

Y

L

L

L

E

E

E

T

A

A

-

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H
|
H

L

-

H

-

R

-

V

A

E

P

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

I

P

P

T

I

G

D

H

H

V

L

S

M

A

G

T

V

P

P

A

I

V

L

A

G

D

E

W

Y

I

F

-

E

A

G

K

K

T

N

V

L

P

E

D

D

A

I

I

V

L

I

I

V

G

S

P

P

D

D

S

H

E

G

S

G

E

V

Q

T

W

R

V

A

A

R

G

K

I

L

A

A

A

D

R

R

S

L

G

K

M

A

P

P

Y

I

Q

A

V

I

L

I

A

-

K

-

E

-

R

-

H

-

G

-

D

-

Y

-

D

D

V

K

R

R

V

M

S

N

L

I

P

-

D

-

P

V

A

G

A

N

V

I

A

E

G

G

R

K

T

T

P

A

V

I

L

L

I

I

D

D

I

I

V

I

S
x
D

S
x
T

G
x
A

H

G

T
|
T

I

I

L

T

E

L

T

A

L

A

A

N

H

A

L

L

R

V

Q

E

L

N

S

G

L

A

P

K

A

E

P

V

M

Y

L

A

V

C

A

C

I

T

H

H

P

P

V

V

F

L

A

S

G

G

D

P

A

A

Y

V

A

E

R

R

L

I

Q

N

E

N

A

S

G

T

L

I

A

K

R

E

I

L

V

V

S

V

T

T

D

N

T

S

I

I

A

K

H

L

P

P

S

E

binding methyl phosphonic acid adenosine ester: R94 (= R94), Q95 (= Q95), R97 (≠ T97), H126 (= H127)
binding sulfate ion: D209 (≠ S222), T210 (≠ S223), A211 (≠ G224), T213 (= T226)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
24% identity, 90% coverage: 5:277/304 of query aligns to 6:266/299 of 1ibsB

query
sites
1ibsB

L

I

M

F

P

S

F

L

P

N

S

S

D

N

A

P

V

E

L

L

A

A

G

K

T

E

I

I

A

A

Q

D

P

I

L

V

Q

G

A

V

R

Q

I

L

S

G

P

K

V

C

A

S

W

V

R

T

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

Q

V

I

T

N

L

I

D

E

T

S

L

I

A

R

G

G

A

C

D

D

V

C

A

Y

I

I

L

I

A

Q

S

S

L

T

R

S

S

D

-

P

A

V

D

N

A

E

L

H

A

I

L

M

P

E

L

L

R

L

F

I

A

M

A

V

L

D

T

A

A

L

R

K

E

R

F

A

G

S

A

A

R

K

S

T

V

I

G

N

L

I

I

V

A

I

P

P

Y

Y

L

Y

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

T
x
R

R

K

F

S

H

R

R

-

G

-

E

E

A

P

V

I

S

T

T

A

P

K

L

L

F

F

A

A

Q

N

Y

L

L

L

E

E

E

T

A

A

-

G

F

A

D

T

W

R

L

V

V

I

T

A

V

L

D

D

P

L

H

H

L

-

H

-

R

-

V

A

E

P

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

I

P

P

T

I

G

D

H

H

V

L

S

M

A

G

T

V

P

P

A

I

V

L

A

G

D

E

W

Y

I

F

-

E

A

G

K

K

T

N

V

L

P

E

D

D

A

I

I

V

L

I

I

V

G

S

P

P

D

D

S

H

E

G

S

G

E

V

Q

T

W

R

V

A

A

R

G

K

I

L

A

A

A

D

R

R

S

L

G

K

M

A

P

P

Y

I

Q

A

V

I

L

I

A

-

K

-

E

-

R

-

H

-

G

-

D

-

Y

-

D

D

V

K

R

R

V

M

S

N

L

I

P

-

D

-

P

V

A

G

A

N

V

I

A

E

G

G

R

K

T

T

P

A

V

I

L

L

I

I

D

D

I

I

V

I

S

D

S

T

G

A

H

G

T

T

I

I

L

T

E

L

T

A

L

A

A

N

H

A

L

L

R

V

Q

E

L

N

S

G

L

A

P

K

A

E

P

V

M

Y

L

A

V

C

A

C

I

T

H

H

P

P

V

V

F

L

A

S

G

G

D

P

A

A

Y

V

A

E

R

R

L

I

Q

N

E

N

A

S

G

T

L

I

A

K

R

E

I

L

V

V

S

V

T

T

D

N

T

S

I

I

A

K

H

L

P

P

S

E

binding methyl phosphonic acid adenosine ester: R96 (= R94), Q97 (= Q95), R99 (≠ T97)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
26% identity, 86% coverage: 14:273/304 of query aligns to 15:275/315 of P0A717

query
sites
P0A717

L

L

A

A

G

Q

T

R

I

I

A

A

Q

N

P

R

L

L

Q

Y

A

T

R

S

I

L

S

G

P

D

V

A

A

A

W

V

R

G

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

T

Q

L

I

D

N

D

E

T

N

L

V

A

R

G

G

A

G

D

D

V

I

A

F

I

I

L

I

A

Q

S

S

L

T

-

C

-

A

-

P

R

T

S

N

A

D

D

N

A

L

L

M

A

E

L

L

P

V

L

V

R

M

F

V

A

D

A

A

L

L

T

-

A

-

R

R

E

R

F

A

G

S

A

A

R

G

S

R

V

I

G

T

L

A

I

V

A

I

P

P

Y

Y

L

F

G

G

Y

Y

M

A

R

R

Q

Q

D

D

T

R

R

R

F

V

H

R

R

S

G

A

E

R

A

V

-

P

V

I

S

T

T

A

P

K

L

V

F

V

A

A

Q

D

Y

F

L

L

E

S

-

V

A

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D
|
D

P

L

H

H

L

-

H

-

R

-

V

A

E

E

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

V

P

P

T

V

G

D

H

N

V

V

S

F

A

G

T

S

P

P

A

I

V

L

-

L

A

E

D

D

W

M

I

L

A

Q

K

L

T

N

V

L

P

D

D

N

A

P

I

I

L

V

I

V

G

S

P

P

D

D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

A

R

G

A

I

I

A

A

A

K

R

L

S

L

G

N

M

D

P

T

Y

D

Q

M

V

A

L

I

A

I

K

D

E

K

R

R

H

R

G

P

D

R

Y

A

D

N

V

V

R

S

V

Q

S

V

L

M

P

H

D

I

P

I

A

G

A

D

V

V

A

A

G

G

R

R

T

D

P

C

V

V

L

L

I

V

D
|
D

I

M

V

I

S
x
D

S

T

G

G

H

G

T

T

I

L

L

C

E

K

T

A

L

A

A

E

H

A

L

L

R

K

Q

E

L

R

S

G

L

A

P

K

A

R

P

V

M

F

L

A

V

Y

A

A

I

T

H

H

P

P

V

I

F

F

A

S

G

G

D

N

A

A

Y

A

A

N

R

N

L

L

Q

R

E

N

A

S

G

V

L

I

A

D

R

E

I

V

V

V

S

V

T

C

D

D

T

T

I

I

D129 (= D125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D218) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D219) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S222) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
26% identity, 86% coverage: 14:273/304 of query aligns to 14:274/308 of 4s2uA

query
sites
4s2uA

L

L

A

A

G

Q

T

R

I

I

A

A

Q

N

P

R

L

L

Q

Y

A

T

R

S

I

L

S

G

P

D

V

A

A

A

W

V

R

G

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

T

Q

L

I

D

N

D

E

T

N

L

V

A

R

G

G

A

G

D

D

V

I

A

F

I

I

L

I

A

Q

S

S

L

T

-

C

-

A

-

P

R

T

S

N

A

D

D

N

A

L

L

M

A

E

L

L

P

V

L

V

R

M

F

V

A

D

A

A

L

L

T

-

A

-

R

R

E

R

F

A

G

S

A

A

R

G

S

R

V

I

G

T

L

A

I

V

A

I

P

P

Y

Y

L

F

G

G

Y

Y

M

A

R

R

Q

Q

D

D

T

R

R

R

F

V

H

R

R

S

G

A

E

R

A

V

-

P

V

I

S

T

T

A

P

K

L

V

F

V

A

A

Q

D

Y

F

L

L

E

S

-

V

A

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D

D

P

L

H

H

L

-

H

-

R

-

V

A

E

E

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

V

P

P

T

V

G

D

H

N

V

V

S

F

A

G

T

S

P

P

A

I

V

L

-

L

A

E

D

D

W

M

I

L

A

Q

K

L

T

N

V

L

P

D

D

N

A

P

I

I

L

V

I

V

G

S

P

P

D

D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

A

R

G

A

I

I

A

A

A

K

R

L

S

L

G

N

M

D

P

T

Y

D

Q

M

V

A

L

I

A

I

K

D

E

K

R

R

H

R

G

P

D

R

Y

A

D

N

V

V

R

S

V

Q

S

V

L

M

P

H

D

I

P

I

A

G

A

D

V

V

A

A

G

G

R

R

T

D

P

C

V

V

L

L

I

V

D

D

I

M

V

I

S
x
D

S

T

G

G

H

G

T
|
T

I

L

L

C

E

K

T

A

L

A

A

E

H

A

L

L

R

K

Q

E

L

R

S

G

L

A

P

K

A

R

P

V

M

F

L

A

V

Y

A

A

I

T

H

H

P

P

V

I

F

F

A

S

G

G

D

N

A

A

Y

A

A

N

R

N

L

L

Q

R

E

N

A

S

G

V

L

I

A

D

R

E

I

V

V

V

S

V

T

C

D

D

T

T

I

I

binding magnesium ion: D223 (≠ S222), T227 (= T226)

6asvC E. Coli prpp synthetase (see paper)
26% identity, 86% coverage: 14:273/304 of query aligns to 13:273/311 of 6asvC

query
sites
6asvC

L

L

A

A

G

Q

T

R

I

I

A

A

Q

N

P

R

L

L

Q

Y

A

T

R

S

I

L

S

G

P

D

V

A

A

A

W

V

R

G

H

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

T

Q

L

I

D

N

D

E

T

N

L

V

A

R

G

G

A

G

D

D

V

I

A

F

I

I

L

I

A

Q

S

S

L

T

-

C

-

A

-

P

R

T

S

N

A

D

D

N

A

L

L

M

A

E

L

L

P

V

L

V

R

M

F

V

A

D

A

A

L

L

T

-

A

-

R

R

E

R

F

A

G

S

A

A

R

G

S

R

V

I

G

T

L

A

I

V

A

I

P

P

Y

Y

L

F

G

G

Y

Y

M

A

R

R

Q

Q

D

D

T

R

R

R

F

V

H

R

R

S

G

A

E

R

A

V

-

P

V

I

S

T

T

A

P

K

L

V

F

V

A

A

Q

D

Y

F

L

L

E

S

-

V

A

G

F

V

D

D

W

R

L

V

V

L

T

T

V

V

D

D

P

L

H

H

L

-

H

-

R

-

V

A

E

E

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

V

P

P

T

V

G

D

H

N

V

V

S

F

A

G

T

S

P

P

A

I

V

L

-

L

A

E

D

D

W

M

I

L

A

Q

K

L

T

N

V

L

P

D

D

N

A

P

I

I

L

V

I

V

G

S

P

P

D
|
D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

A

R

G

A

I

I

A

A

A

K

R

L

S

L

G

N

M

D

P

T

Y

D

Q

M

V

A

L

I

A

I

K

D

E

K

R

R

H

R

G

P

D

R

Y

A

D

N

V

V

R

S

V

Q

S

V

L

M

P

H

D

I

P

I

A

G

A

D

V

V

A

A

G

G

R

R

T

D

P

C

V

V

L

L

I

V

D
|
D

I

M

V

I

S
x
D

S
x
T

G
|
G

H

G

T
|
T

I
x
L

L

C

E

K

T

A

L

A

A

E

H

A

L

L

R

K

Q

E

L

R

S

G

L

A

P

K

A

R

P

V

M

F

L

A

V

Y

A

A

I

T

H

H

P

P

V

I

F

F

A

S

G

G

D

N

A

A

Y

A

A

N

R

N

L

L

Q

R

E

N

A

S

G

V

L

I

A

D

R

E

I

V

V

V

S

V

T

C

D

D

T

T

I

I

binding phosphate ion: D168 (= D168), D218 (= D218), D219 (= D219), D222 (≠ S222), T223 (≠ S223), G224 (= G224), T226 (= T226), L227 (≠ I227)

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
28% identity, 91% coverage: 5:282/304 of query aligns to 2:274/300 of 3dahC

query
sites
3dahC

L

L

M

M

P

V

F

F

P

T

S

G

D

N

A

A

-

N

-

P

V

A

L

L

A

A

G

Q

T

E

I

V

A

V

Q

K

P

I

L

L

Q

G

A

I

R

P

I

L

S

G

P

K

V

A

A

M

W

V

R

S

H

R

F
|
F

P

S

D
|
D

G

G

E
|
E

S

I

L

Q

V

V

T

E

L

I

D

Q

D

E

T

N

L

V

A

R

G

G

A

K

D

D

V

V

A

F

I

V

L

L

A

Q

S

S

L

T

-

C

-

A

-

P

R

T

S

N

A

D

D

N

A

L

L

M

A

E

L

L

P

M

L

I

R

M

F

V

A

D

A

A

L

L

T

K

A

R

R

A

E

S

F

A

G

G

A

R

R

I

S

T

V

A

G

A

L

I

I

-

A

-

P

P

Y

Y

L

F

G

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

T

R

R

R

F

P

H

R

R

S

G

A

E

R

-

V

A

A

V

I

S

S

T

A

P

K

L

V

F

V

A

A

Q

N

Y

M

L

L

E

E

E

I

A

A

-

G

F

V

D

E

W

R

L

I

V

I

T

T

V

M

D

D

P

L

H

H

L

-

H

-

R

-

V

A

E

D

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

I

P

P

T

V

G

D

H

N

V

I

S

Y

A

A

T

T

P

P

A

I

V

L

-

L

A

G

D

D

W

L

I

R

A

K

K

Q

T

N

V

Y

P

P

D

D

A

L

I

L

L

V

I

V

G

S

P

P

D

D

S

-

E

-

S

-

E

-

Q

-

W

-

V

V

A

G

G

G

I

V

A

-

R

-

S

-

G

-

M

V

P

R

Y

A

Q

R

V

A

L

L

A

A

K

K

E

Q

R

L

H

N

G

C

D

D

Y

L

-

A

-

I

D

D

V

K

R

R

V

R

S

V

L

M

P

N

D

I

P

I

A

G

A

E

V

V

A

E

G

G

R

R

T

T

P

C

V

V

L

I

I

M

D

D

I

M

V

V

S

D

S

T

G

A

H

G

T

T

I

L

L

C

E

K

T

A

L

A

A

Q

H

V

L

L

R

K

Q

E

L

R

S

G

L

A

P

K

A

Q

P

V

M

F

L

A

V

Y

A

A

I

T

H

H

P

P

V

V

F

L

A

S

G

G

D

G

A

A

Y

A

A

D

R

R

L

I

Q

A

E

A

A

S

G

A

L

L

A

D

R

E

I

L

V

V

S

V

T

T

D

D

T

T

I

I

A

P

H

L

P

S

S

A

N

E

A

S

I

L

A

A

L

C

binding adenosine monophosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R94), Q95 (= Q95)

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 86% coverage: 14:273/304 of query aligns to 23:285/326 of P9WKE3

query
sites
P9WKE3

L

L

A

A

G

E

T

Q

I

V

A

A

Q
x
K

P

E

L

L

Q

D

A

V

R

H

I

V

S

T

P

S

V

Q

A

D

W

A

R

R

H

E

F

F

P

A

D

N

G

G

E

E

S

I

L

F

V

V

T

R

L

F

D

H

D

E

T

S

L

V

A

R

G

G

A

C

D

D

V

A

A

F

I

V

L

L

A

Q

S

S

L

C

R

P

S

A

A

P

D

V

A

N

L

R

A

W

L

L

P

M

L

E

R

Q

F

L

A

I

A

M

L

I

T

D

A

A

R

L

E

K

F

R

G

G

-

S

A

A

R

K

S

R

V

I

G

T

L

A

I

V

A

M

P

P

Y

F

L

Y

G

P

Y

Y

M

A

R

R

Q

Q

D

D

T

K

R

K

F

-

H

H

R

R

G

G

-

R

E

E

A

P

V

I

S

S

T

A

P

R

L

L

F

I

A

A

Q

D

Y

L

L

L

E

K

E

T

A

A

F

G

-

A

D

D

W

R

L

I

V

V

T

T

V

V

D

D

P

L

H

H

L

-

H

-

R

-

V

T

E

D

R

Q

L

I

Q

Q

S

G

L

F

Y

F

R

D

I

G

P

P

T

V

G

D

H

H

V

M

S

R

A

G

T

Q

P

N

A

L

V

L

A

T

D

G

W

Y

I

I

A

R

K

D

T

N

V

Y

P

P

D

D

A

G

-

N

-

M

I

V