SitesBLAST
Comparing WP_066922393.1 NCBI__GCF_001579945.1:WP_066922393.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 79% coverage: 21:298/351 of query aligns to 11:274/291 of 6n2oD
- binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
- binding succinyl-coenzyme a: I57 (= I66), R62 (≠ K71), L134 (= L145), K136 (= K147)
- binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), C59 (= C68), C208 (= C218), T210 (= T220), F211 (= F221)
- binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), G133 (= G144), L134 (= L145), T135 (= T146)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 79% coverage: 21:298/351 of query aligns to 11:274/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (≠ K71), L134 (= L145)
- binding coenzyme a: K136 (= K147), Y150 (≠ G157)
- binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
- binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), C59 (= C68), C208 (= C218), T210 (= T220), F211 (= F221)
- binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), Y132 (= Y143), G133 (= G144), L134 (= L145), T135 (= T146)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
33% identity, 79% coverage: 21:298/351 of query aligns to 11:274/291 of 6n2nB
- binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
- binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), H30 (= H39), C59 (= C68), C208 (= C218), T210 (= T220), F211 (= F221)
- binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G100 (= G111), D101 (= D112), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), Y132 (= Y143), G133 (= G144), L134 (= L145), T135 (= T146)
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
38% identity, 67% coverage: 34:267/351 of query aligns to 9:251/301 of 5b46B
- binding magnesium ion: D87 (= D112), N115 (= N140), V117 (= V142)
- binding iron/sulfur cluster: C9 (= C34), C12 (= C37), C43 (= C68), C194 (= C218), T196 (= T220), Y197 (≠ F221)
- binding thiamine diphosphate: I41 (= I66), G42 (= G67), C43 (= C68), S44 (= S69), H62 (= H87), G86 (= G111), G88 (= G113), D89 (= D114), N115 (= N140), V117 (= V142), Y118 (= Y143), G119 (= G144), L120 (= L145), T121 (= T146)
Sites not aligning to the query:
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
38% identity, 67% coverage: 34:267/351 of query aligns to 12:254/304 of Q96XT4
- C12 (= C34) binding [4Fe-4S] cluster
- C15 (= C37) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 66:69) binding thiamine diphosphate
- C46 (= C68) binding [4Fe-4S] cluster
- D90 (= D112) binding Mg(2+)
- GD 91:92 (= GD 113:114) binding thiamine diphosphate
- N118 (= N140) binding Mg(2+)
- V120 (= V142) binding Mg(2+)
- GL 122:123 (= GL 144:145) binding thiamine diphosphate
- C197 (= C218) binding [4Fe-4S] cluster
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
36% identity, 77% coverage: 26:294/351 of query aligns to 4:276/305 of Q96Y68
- C12 (= C34) binding [4Fe-4S] cluster
- C15 (= C37) binding [4Fe-4S] cluster
- C46 (= C68) binding [4Fe-4S] cluster
- K49 (= K71) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D112) binding Mg(2+)
- GD 91:92 (= GD 113:114) binding thiamine diphosphate
- N118 (= N140) binding Mg(2+)
- V120 (= V142) binding Mg(2+)
- GL 122:123 (= GL 144:145) binding thiamine diphosphate
- K125 (= K147) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (≠ E194) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C218) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
36% identity, 75% coverage: 30:294/351 of query aligns to 8:276/305 of P72579
- K49 (= K71) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (= L145) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
35% identity, 67% coverage: 34:267/351 of query aligns to 5:225/275 of 5b47B
- binding magnesium ion: D83 (= D112), N111 (= N140)
- binding iron/sulfur cluster: C5 (= C34), C8 (= C37), C39 (= C68), C179 (= C218)
- binding thiamine diphosphate: I37 (= I66), G38 (= G67), C39 (= C68), S40 (= S69), H58 (= H87), G84 (= G113), D85 (= D114), N111 (= N140), V113 (= V142), Y114 (= Y143), L116 (= L145)
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
40% identity, 61% coverage: 30:242/351 of query aligns to 4:209/286 of 5b48B
- binding magnesium ion: D84 (= D112), N112 (= N140), V114 (= V142)
- binding iron/sulfur cluster: C8 (= C34), C11 (= C37), C42 (= C68), C183 (= C218), T185 (= T220)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I66), G41 (= G67), C42 (= C68), S43 (= S69), H59 (= H87), G85 (= G113), D86 (= D114), N112 (= N140), V114 (= V142), Y115 (= Y143), G116 (= G144), L117 (= L145)
Query Sequence
>WP_066922393.1 NCBI__GCF_001579945.1:WP_066922393.1
MSFIAKPKVAHPSLPRNALGLTRRDYEGGMSTLCAGCGHDSVTAAIVEAIWEMSIRPEQL
VKLSGIGCSSKTTAYFDSGGHGFNSVHGRMPSIATGAISANRDLHYVGVSGDGDTLSIGF
GQFAHAIRRNLNMLYIIENNGVYGLTKGQFSASADIGTKAKRGEENRQPPVDPVLTALTL
GGSFIARSFSGDKEQLVPLIMAGLRHRGFALIDVLSPCVTFNDHQGSTKSYSFIRENYDA
VVHADFIAPAQEITASYAAGEAMSVAMHDGSRILLRKLDPSYDPTDRTRAYAYLRSRLAQ
GEYVTGLIHIDESSPDFHEVSGSSTTPMNQLPYARLSPGSEGLKKILNRYR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory