SitesBLAST
Comparing WP_067075467.1 NCBI__GCF_001602375.1:WP_067075467.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
38% identity, 98% coverage: 5:388/391 of query aligns to 10:399/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: R23 (≠ K18), G40 (= G35), L41 (= L36)
- binding 2-oxoglutaric acid: D213 (= D204), P214 (≠ A205), Y215 (≠ F206), G216 (= G207), E217 (= E208), G241 (= G231), T242 (≠ S232), I246 (= I236)
- binding (2E)-pent-2-enedioic acid: G40 (= G35), Y130 (= Y126), N184 (= N175), R376 (= R365)
- binding glutamic acid: S20 (= S15), V22 (≠ L17), L131 (= L127), V360 (≠ L349), A364 (≠ P353), R369 (≠ G358)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (≠ F206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
38% identity, 98% coverage: 5:388/391 of query aligns to 10:399/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: S20 (= S15), V22 (≠ L17), R23 (≠ K18), L131 (= L127), Q135 (≠ E131), A364 (≠ P353), R369 (≠ G358)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: R23 (≠ K18), G40 (= G35), Y130 (= Y126), L131 (= L127), A132 (≠ G128), N184 (= N175), R376 (= R365)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (≠ I170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (≠ F206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
38% identity, 98% coverage: 5:388/391 of query aligns to 10:399/404 of 3aowC
- binding 2-oxoglutaric acid: R23 (≠ K18), Y70 (= Y65), Y130 (= Y126), L275 (= L265)
- binding pyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (≠ I170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (≠ F206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
38% identity, 98% coverage: 5:388/391 of query aligns to 10:399/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (≠ I170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (≠ F206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
40% identity, 99% coverage: 5:390/391 of query aligns to 10:393/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I22 (≠ L17), R23 (≠ K18), G39 (= G34), G40 (= G35), G99 (= G100), S100 (= S101), Q101 (= Q102), Y125 (= Y126), N174 (= N175), D202 (= D203), Y205 (≠ F206), S235 (= S232), S237 (= S234), K238 (= K235), R245 (= R242), R368 (= R365)
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
40% identity, 99% coverage: 5:390/391 of query aligns to 10:393/397 of Q72LL6
- S20 (= S15) mutation to E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- R23 (≠ K18) mutation to A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; mutation to Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
- G40 (= G35) binding substrate
- Y70 (= Y65) binding pyridoxal 5'-phosphate
- N174 (= N175) binding pyridoxal 5'-phosphate; binding substrate
- R245 (= R242) binding pyridoxal 5'-phosphate
- R368 (= R365) binding substrate
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
40% identity, 99% coverage: 5:390/391 of query aligns to 6:389/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: I18 (≠ L17), R19 (≠ K18), G35 (= G34), G36 (= G35), G95 (= G100), S96 (= S101), Q97 (= Q102), Y121 (= Y126), N170 (= N175), D198 (= D203), Y201 (≠ F206), S231 (= S232), S233 (= S234), K234 (= K235), R241 (= R242), R364 (= R365)
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
40% identity, 99% coverage: 5:390/391 of query aligns to 6:389/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G100), S96 (= S101), Q97 (= Q102), Y121 (= Y126), N170 (= N175), D198 (= D203), A200 (= A205), Y201 (≠ F206), S231 (= S232), S233 (= S234), K234 (= K235), R241 (= R242)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
39% identity, 98% coverage: 5:388/391 of query aligns to 7:397/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G100), S103 (= S101), Q104 (= Q102), Y128 (= Y126), V177 (≠ I170), N182 (= N175), D210 (= D203), P212 (≠ A205), Y213 (≠ F206), T240 (≠ S232), S242 (= S234), K243 (= K235), R250 (= R242)
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
40% identity, 99% coverage: 5:390/391 of query aligns to 7:385/389 of 2z1yA
- binding leucine: G32 (= G35), Y117 (= Y126), R360 (= R365)
- binding pyridoxal-5'-phosphate: G91 (= G100), S92 (= S101), Q93 (= Q102), Y117 (= Y126), N166 (= N175), D194 (= D203), Y197 (≠ F206), S227 (= S232), S229 (= S234), K230 (= K235), R237 (= R242)
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
35% identity, 97% coverage: 7:387/391 of query aligns to 17:410/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G100), S113 (= S101), Q114 (= Q102), Y138 (= Y126), N194 (= N175), D222 (= D203), P224 (≠ A205), Y225 (≠ F206), T252 (≠ S232), S254 (= S234), K255 (= K235), R262 (= R242)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
34% identity, 92% coverage: 29:388/391 of query aligns to 36:402/405 of 2zc0A