SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_067473665.1 NCBI__GCF_001659785.1:WP_067473665.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
35% identity, 98% coverage: 8:453/457 of query aligns to 11:460/467 of 7qh2C

query
sites
7qh2C

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binding flavin-adenine dinucleotide: V73 (≠ P71), G75 (= G73), S76 (≠ A74), G77 (= G75), T78 (≠ S76), G79 (= G77), L80 (= L78), A83 (≠ G81), C84 (≠ A82), P137 (= P134), G138 (≠ S135), E139 (≠ S136), A142 (≠ C140), T143 (≠ S141), G146 (= G144), N147 (= N145), S149 (≠ A147), T150 (= T148), A152 (= A150), G153 (= G151), E203 (= E201), G204 (= G202), I209 (= I207), E422 (= E415), H423 (= H416)
binding fe (iii) ion: H377 (= H371), H384 (= H378), E422 (= E415)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 98% coverage: 11:457/457 of query aligns to 8:456/459 of P9WIT1

query
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P9WIT1

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K354 (≠ Q350) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:455/455 of 8jdxA

query
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8jdxA

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L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding flavin-adenine dinucleotide: E32 (≠ F34), P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W322 (vs. gap), E413 (= E415), H414 (= H416), N450 (= N452)
binding 3-methyl-2-oxobutanoic acid: R318 (≠ A322), H369 (= H371), H376 (= H378), H414 (= H416)
binding manganese (ii) ion: H369 (= H371), H376 (= H378), E413 (= E415)

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:456/456 of 8jdsA

query
sites
8jdsA

I

V

D

E

A

A

L

L

R

K

E

A

R

V

I

V

P

G

S

S

S

P

P

H

I

V

L

S

T

T

D

A

P

S

D

A

V

V

T

R

E

E

G

Q

Y

H

R

G

R

H

D

D

F
x
E

A

S

K

M

D

H

P

-

D

R

A

C

G

Q

R

P

P

P

L

D

A

A

V

V

R

W

A

P

T

Q

S

N

T

V

A

D

D

Q

V

V

Q

S

E

R

V

V

M

A

C

S

W

L

A

C

S

Y

E

N

N

Q

A

G

I

V

A

P

V

I

I

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

S
x
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

A

A

V

V

T

Q

G

G

I

V

V

C

L

I

T

N

-
x
L

T

T

E

H

L

M

M

D

Q

Q

D

I

I

T

E

E

V

L

D

N

P

T

V

E

T

D

R

F

T

S

A

V

V

V

Q

E

P

P

G

G

L

V

L

T

N

R

A

K

E

A

V

L

K

N

K

T

A

H

V

L

A

R

E

D

H

S

G

G

L

L

W

W

Y

F

P

P

P

V

D

D

P
|
P

S
x
G

S
x
A

F

-

E

-

I

D

C

A

S

S

I

L

G

C

G
|
G

N
x
M

A

A

A
|
A

T
|
T

N

G

A
|
A

G
x
S

G

G

L

T

C

N

C

A

V

V

K

R

Y

Y

G

G

V

T

T

M

T

R

D

D

Y

N

I

V

L

I

G

N

M

L

E

E

V

V

L

L

A

P

D

D

G

G

K

R

A

L

V

L

R

H

L

T

G

A

G

G

-

R

-

G

-

R

P

H

R

Y

L

R

K

K

D

S

S

A

A

A

G

G

F

Y

S

N

L

L

T

T

K

G

L

L

F

F

V

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

I
|
I

T

T

E

S

V

T

I

T

I

L

R

R

L

L

I

H

P

P

K

-

Q

-

A

A

P

P

A

E

S

A

T

T

V

V

V

A

G

A

T

T

-

C

-

A

F

F

P

P

T

S

V

V

A

Q

D

A

S

A

T

V

Q

D

A

S

I

T

L

V

D

Q

I

I

T

L

R

Q

V

A

M

A

R

V

P

P

-

V

A

A

M

R

L

I

E

E

F

F

M

L

D

D

R

D

A

V

S

M

I

M

R

D

A

A

V

C

E

N

D

R

V

H

K

S

R

K

M

L

G

N

L

C

D

P

V

V

D

A

A

P

H

-

G

-

M

T

L

L

I

F

A

L

Q

E

S

F

D

H

S

G

P

S

G

Q

A

Q

D

T

C

L

S

A

R

E

E

Q

V

L

E

Q

F

-

M

R

A

T

E

E

A

A

F

I

R

T

N

Q

N

D

N

N

A

G

D

S

V

H

F

F

T

S

T

W

D

A

P

E

E

K

E

R

G

N

E

E

A

L

F

W

A

A

A
x
R

R

H

R

N

-

A

-
x
W

F

Y

A

A

I

A

P

L

A

A

V

L

E

S

R

P

K

G

G

S

T

K

L

A

L

Y

L

S

E

T

D

D

V

V

G

C

V

V

P

P

L

I

P

S

A

R

L

L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding flavin-adenine dinucleotide: E32 (≠ F34), P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W323 (vs. gap), E414 (= E415), H415 (= H416), N451 (= N452)
binding manganese (ii) ion: H370 (= H371), H377 (= H378), E414 (= E415)
binding pyruvic acid: R319 (≠ A322), H370 (= H371), H377 (= H378), H415 (= H416)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:454/454 of 8jdvA

query
sites
8jdvA

I

V

D

E

A

A

L

L

R

K

E

A

R

V

I

V

P

G

S

S

S

P

P

H

I

V

L

S

T

T

D

A

P

S

D

A

V

V

T

R

E

E

G

Q

Y

H

R

G

R

H

D

D

F

E

A

S

K

M

D

H

P

-

D

R

A

C

G

Q

R

P

P

P

L

D

A

A

V

V

R

W

A

P

T

Q

S

N

T

V

A

D

D

Q

V

V

Q

S

E

R

V

V

M

A

C

S

W

L

A

C

S

Y

E

N

N

Q

A

G

I

V

A

P

V

I

I

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

S
x
T

G
|
G

L
x
V

S

E

G

G

G
|
G

A
x
V

T

C

A

A

V

V

T

Q

G

G

I

V

V

C

L

I

T

N

-
x
L

T

T

E

H

L

M

M

D

Q

Q

D

I

I

T

E

E

V

L

D

N

P

T

V

E

T

D

R

F

T

S

A

V

V

V

Q

E

P

P

G

G

L

V

L

T

N

R

A

K

E

A

V

L

K

N

K

T

A

H

V

L

A

R

E

D

H

S

G

G

L

L

W

W

Y

F

P

P

P

V

D

D

P
|
P

S
x
G

S
x
A

F

-

E

-

I

D

C

A

S

S

I

L

G

C

G
|
G

N
x
M

A

A

A
|
A

T
|
T

N

G

A
|
A

G
x
S

G

G

L

T

C

N

C

A

V

V

K

R

Y

Y

G

G

V

T

T

M

T

R

D

D

Y

N

I

V

L

I

G

N

M

L

E

E

V

V

L

L

A

P

D

D

G

G

K

R

A

L

V

L

R

H

L

T

G

A

G

G

-

R

-

G

-

R

P

H

R

Y

L

R

K

K

D

S

S

A

A

A

G

G

F

Y

S

N

L

L

T

T

K

G

L

L

F

F

V

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

I
|
I

T

T

E

S

V

T

I

T

I

L

R

R

L

L

I

H

P

P

K

-

Q

-

A

A

P

P

A

E

S

A

T

T

V

V

V

A

G

A

T

T

-

C

-

A

F

F

P

P

T

S

V

V

A

Q

D

A

S

A

T

V

Q

D

A

S

I

T

L

V

D

Q

I

I

T

L

R

Q

V

A

M

A

R

V

P

P

-

V

A

A

M

R

L

I

E

E

F

F

M

L

D

D

R

D

A

V

S

M

I

M

R

D

A

A

V

C

E

N

D

R

V

H

K

S

R

K

M

L

G

N

L

C

D

P

V

V

D

A

A

P

H

-

G

-

M

T

L

L

I

F

A

L

Q

E

S

F

D

H

S

G

P

S

G

Q

A

Q

D

T

C

L

S

A

R

E

E

Q

V

L

E

Q

F

-

M

R

A

T

E

E

A

A

F

I

R

T

N

Q

N

D

N

N

A

G

D

S

V

H

F

F

T

S

T

A

D

E

D

K

P

R

E

N

E

E

G

L

E

W

A

A

F

A

A
x
R

A

H

A

N

R

A

R
x
W

F
x
Y

A

A

I

A

P

L

A

A

V

L

E

S

R

P

K

G

G

S

T

K

L

A

L

Y

L

S

E

T

D

D

V

V

G

C

V

V

P

P

L

I

P

S

A

R

L

L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding 2-Ketohexanoic acid: V75 (≠ L78), R317 (≠ A320), W321 (≠ R324), H368 (= H371), H375 (= H378), H413 (= H416)
binding flavin-adenine dinucleotide: P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W321 (≠ R324), Y322 (≠ F325), E412 (= E415), H413 (= H416), N449 (= N452)
binding manganese (ii) ion: H368 (= H371), H375 (= H378), E412 (= E415)

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:455/455 of 8jdeA

query
sites
8jdeA

I

V

D

E

A

A

L

L

R

K

E

A

R

V

I

V

P

G

S

S

S

P

P

H

I

V

L

S

T

T

D

A

P

S

D

A

V

V

T

R

E

E

G

Q

Y

H

R

G

R

H

D

D

F

E

A

S

K

M

D

H

P

-

D

R

A

C

G

Q

R

P

P

P

L

D

A

A

V

V

R

W

A

P

T

Q

S

N

T

V

A

D

D

Q

V

V

Q

S

E

R

V

V

M

A

C

S

W

L

A

C

S

Y

E

N

N

Q

A

G

I

V

A

P

V

I

I

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

S
x
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

A

A

V

V

T

Q

G

G

I

V

V

C

L

I

T

N

-
x
L

T

T

E

H

L

M

M

D

Q

Q

D

I

I

T

E

E

V

L

D

N

P

T

V

E

T

D

R

F

T

S

A

V

V

V

Q

E

P

P

G

G

L

V

L

T

N

R

A

K

E

A

V

L

K

N

K

T

A

H

V

L

A

R

E

D

H

S

G

G

L

L

W

W

Y

F

P

P

P

V

D

D

P
|
P

S
x
G

S
x
A

F

-

E

-

I

D

C

A

S

S

I

L

G

C

G
|
G

N
x
M

A

A

A
|
A

T
|
T

N

G

A
|
A

G
x
S

G

G

L

T

C

N

C

A

V

V

K

R

Y

Y

G

G

V

T

T

M

T

R

D

D

Y

N

I

V

L

I

G

N

M

L

E

E

V

V

L

L

A

P

D

D

G

G

K

R

A

L

V

L

R

H

L

T

G

A

G

G

-

R

-

G

-

R

P

H

R

Y

L

R

K

K

D

S

S

A

A

A

G

G

F

Y

S

N

L

L

T

T

K

G

L

L

F

F

V

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

I
|
I

T

T

E

S

V

T

I

T

I

L

R

R

L

L

I

H

P

P

K

-

Q

-

A

A

P

P

A

E

S

A

T

T

V

V

V

A

G

A

T

T

-

C

-

A

F

F

P

P

T

S

V

V

A

Q

D

A

S

A

T

V

Q

D

A

S

I

T

L

V

D

Q

I

I

T

L

R

Q

V

A

M

A

R

V

P

P

-

V

A

A

M

R

L

I

E

E

F

F

M

L

D

D

R

D

A

V

S

M

I

M

R

D

A

A

V

C

E

N

D

R

V

H

K

S

R

K

M

L

G

N

L

C

D

P

V

V

D

A

A

P

H

-

G

-

M

T

L

L

I

F

A

L

Q

E

S

F

D

H

S

G

P

S

G

Q

A

Q

D

T

C

L

S

A

R

E

E

Q

V

L

E

Q

F

-

M

R

A

T

E

E

A

A

F

I

R

T

N

Q

N

D

N

N

A

G

D

S

V

H

F

F

T

S

T

W

D

A

D

-

P

A

E

E

E

K

G

N

E

E

A

L

F

W

A

A

A
x
R

R

H

R

N

-

A

-
x
W

F

Y

A

A

I

A

P

L

A

A

V

L

E

S

R

P

K

G

G

S

T

K

L

A

L

Y

L

S

E

T

D

D

V

V

G

C

V

V

P

P

L

I

P

S

A

R

L

L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding flavin-adenine dinucleotide: P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W322 (vs. gap), E413 (= E415), H414 (= H416), N450 (= N452)
binding lactic acid: R318 (≠ A322), H369 (= H371), H376 (= H378), H414 (= H416)
binding manganese (ii) ion: H369 (= H371), H376 (= H378), E413 (= E415)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:455/455 of 8jdtA

query
sites
8jdtA

I

V

D

E

A

A

L

L

R

K

E

A

R

V

I

V

P

G

S

S

S

P

P

H

I

V

L

S

T

T

D

A

P

S

D

A

V

V

T

R

E

E

G

Q

Y

H

R

G

R

H

D

D

F

E

A

S

K

M

D

H

P

-

D

R

A

C

G

Q

R

P

P

P

L

D

A

A

V

V

R

W

A

P

T

Q

S

N

T

V

A

D

D

Q

V

V

Q

S

E

R

V

V

M

A

C

S

W

L

A

C

S

Y

E

N

N

Q

A

G

I

V

A

P

V

I

I

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

S
x
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

A

A

V

V

T

Q

G

G

I

V

V

C

L

I

T

N

-
x
L

T

T

E

H

L

M

M

D

Q

Q

D

I

I

T

E

E

V

L

D

N

P

T

V

E

T

D

R

F

T

S

A

V

V

V

Q

E

P

P

G

G

L

V

L

T

N

R

A

K

E

A

V

L

K

N

K

T

A

H

V

L

A

R

E

D

H

S

G

G

L

L

W

W

Y

F

P

P

P

V

D

D

P
|
P

S
x
G

S
x
A

F

-

E

-

I

D

C

A

S

S

I

L

G

C

G
|
G

N
x
M

A

A

A
|
A

T
|
T

N

G

A
|
A

G
x
S

G

G

L

T

C

N

C

A

V

V

K

R

Y

Y

G

G

V

T

T

M

T

R

D

D

Y

N

I

V

L

I

G

N

M

L

E

E

V

V

L

L

A

P

D

D

G

G

K

R

A

L

V

L

R

H

L

T

G

A

G

G

-

R

-

G

-

R

P

H

R

Y

L

R

K

K

D

S

S

A

A

A

G

G

F

Y

S

N

L

L

T

T

K

G

L

L

F

F

V

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

I
|
I

T

T

E

S

V

T

I

T

I

L

R

R

L

L

I

H

P

P

K

-

Q

-

A

A

P

P

A

E

S

A

T

T

V

V

V

A

G

A

T

T

-

C

-

A

F

F

P

P

T

S

V

V

A

Q

D

A

S

A

T

V

Q

D

A

S

I

T

L

V

D

Q

I

I

T

L

R

Q

V

A

M

A

R

V

P

P

-

V

A

A

M

R

L

I

E

E

F

F

M

L

D

D

R

D

A

V

S

M

I

M

R

D

A

A

V

C

E

N

D

R

V

H

K

S

R

K

M

L

G

N

L

C

D

P

V

V

D

A

A

P

H

-

G

-

M

T

L

L

I

F

A

L

Q

E

S

F

D

H

S

G

P

S

G

Q

A

Q

D

T

C

L

S

A

R

E

E

Q

V

L

E

Q

F

-

M

R

A

T

E

E

A

A

F

I

R

T

N

Q

N

D

N

N

A

G

D

S

V

H

F

F

T

S

T

W

D

A

D

E

P

-

E

K

E

R

G

N

E

E

A

L

F

W

A

A

A
x
R

R

H

R

N

-

A

-
x
W

F

Y

A

A

I

A

P

L

A

A

V

L

E

S

R

P

K

G

G

S

T

K

L

A

L

Y

L

S

E

T

D

D

V

V

G

C

V

V

P

P

L

I

P

S

A

R

L

L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding 2-ketobutyric acid: R318 (≠ A322), H369 (= H371), H376 (= H378), H414 (= H416)
binding flavin-adenine dinucleotide: P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W322 (vs. gap), E413 (= E415), H414 (= H416), N450 (= N452)
binding manganese (ii) ion: H369 (= H371), H376 (= H378), E413 (= E415)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:455/455 of 8jduA

query
sites
8jduA

I

V

D

E

A

A

L

L

R

K

E

A

R

V

I

V

P

G

S

S

S

P

P

H

I

V

L

S

T

T

D

A

P

S

D

A

V

V

T

R

E

E

G

Q

Y

H

R

G

R

H

D

D

F

E

A

S

K

M

D

H

P

-

D

R

A

C

G

Q

R

P

P

P

L

D

A

A

V

V

R

W

A

P

T

Q

S

N

T

V

A

D

D

Q

V

V

Q

S

E

R

V

V

M

A

C

S

W

L

A

C

S

Y

E

N

N

Q

A

G

I

V

A

P

V

I

I

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

S
x
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

A

A

V

V

T

Q

G

G

I

V

V

C

L

I

T

N

-
x
L

T

T

E

H

L

M

M

D

Q

Q

D

I

I

T

E

E

V

L

D

N

P

T

V

E

T

D

R

F

T

S

A

V

V

V

Q

E

P

P

G

G

L

V

L

T

N

R

A

K

E

A

V

L

K

N

K

T

A

H

V

L

A

R

E

D

H

S

G

G

L

L

W

W

Y

F

P

P

P

V

D

D

P
|
P

S
x
G

S
x
A

F

-

E

-

I

D

C

A

S

S

I

L

G

C

G
|
G

N
x
M

A

A

A
|
A

T
|
T

N

G

A
|
A

G
x
S

G

G

L

T

C

N

C

A

V

V

K

R

Y

Y

G

G

V

T

T

M

T

R

D

D

Y

N

I

V

L

I

G

N

M

L

E

E

V

V

L

L

A

P

D

D

G

G

K

R

A

L

V

L

R

H

L

T

G

A

G

G

-

R

-

G

-

R

P

H

R

Y

L

R

K

K

D

S

S

A

A

A

G

G

F

Y

S

N

L

L

T

T

K

G

L

L

F

F

V

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

I
|
I

T

T

E

S

V

T

I

T

I

L

R

R

L

L

I

H

P

P

K

-

Q

-

A

A

P

P

A

E

S

A

T

T

V

V

V

A

G

A

T

T

-

C

-

A

F

F

P

P

T

S

V

V

A

Q

D

A

S

A

T

V

Q

D

A

S

I

T

L

V

D

Q

I

I

T

L

R

Q

V

A

M

A

R

V

P

P

-

V

A

A

M

R

L

I

E

E

F

F

M

L

D

D

R

D

A

V

S

M

I

M

R

D

A

A

V

C

E

N

D

R

V

H

K

S

R

K

M

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

D

F

V

L

D

E

A

F

H

H

G

G

M

-

L

-

I

-

A

-

Q

-

S

S

D

Q

S

Q

P

T

G

L

A

A

D

E

C

Q

S

L

R

Q

E

R

V

T

E

E

F

A

M

I

A

T

E

Q

A

-

F

-

R

-

N

-

N

N

A

G

D

S

V

H

F

F

T

S

T

W

D

A

P

E

E

K

E

R

G

N

E

E

A

L

F

W

A

A

A
x
R

R

H

R

N

-

A

-
x
W

F

Y

A

A

I

A

P

L

A

A

V

L

E

S

R

P

K

G

G

S

T

K

L

A

L

Y

L

S

E

T

D

D

V

V

G

C

V

V

P

P

L

I

P

S

A

R

L

L

P

P

Q

E

-

I

L

L

I

V

E

E

R

T

I

K

E

E

I

I

A

K

A

A

E

S

R

K

D

-

V

L

L

T

I

G

A

A

V

I

I

V

A

G

H
|
H

A

V

G

G

D

D

G

G

N

N

T

F

H
|
H

P

C

L

I

I

L

V

L

H

V

D

D

P

P

N

D

D

D

A

A

E

E

T

Q

A

R

R

R

A

V

N

K

L

-

A

A

F

F

G

A

E

E

I

N

M

L

D

G

F

R

-

R

A

A

I

L

S

A

L

L

G

G

T

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

R

L

L

G

K

K

K

R

N

Q

W

L

L

L

P

Q

S

E

Q

E

V

V

G

G

E

P

D

V

V

G

M

V

D

E

L

T

T

M

R

R

R

Q

I

L

K

K

S

N

A

T

L

L

D

D

P

P

Q

R

N

G

L

L

L

M

N
|
N

P

P

G

G

A

K

M

V

F

L

binding 2-oxopentanoic acid: R318 (≠ A322), W322 (vs. gap), H369 (= H371), H376 (= H378), H414 (= H416)
binding flavin-adenine dinucleotide: P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W322 (vs. gap), E413 (= E415), N450 (= N452)
binding manganese (ii) ion: H369 (= H371), H376 (= H378), E413 (= E415)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:454/454 of 8jdzA

query
sites
8jdzA

I

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E

A

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L

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H

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L

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L

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Q

R

N

G

L

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N
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N

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P

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G

A

K

M

V

F

L

binding (3S)-3-methyl-2-oxopentanoic acid: R318 (≠ A322), W322 (vs. gap), H369 (= H371), H376 (= H378), H413 (= H416)
binding flavin-adenine dinucleotide: E32 (≠ F34), P68 (= P71), G70 (= G73), T71 (≠ A74), G72 (= G75), T73 (≠ S76), G74 (= G77), G78 (= G81), V79 (≠ A82), L90 (vs. gap), P132 (= P134), G133 (≠ S135), A134 (≠ S136), G140 (= G144), M141 (≠ N145), A143 (= A147), T144 (= T148), A146 (= A150), S147 (≠ G151), E200 (= E201), G201 (= G202), I206 (= I207), W322 (vs. gap), E412 (= E415), H413 (= H416), N449 (= N452)
binding manganese (ii) ion: H369 (= H371), H376 (= H378), E412 (= E415)

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
34% identity, 98% coverage: 8:457/457 of query aligns to 6:454/454 of 8jdyA

query
sites
8jdyA

I

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G
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A
x
V

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A

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