SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_067643566.1 NCBI__GCF_001632775.1:WP_067643566.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
33% identity, 97% coverage: 10:670/682 of query aligns to 4:687/707 of 6yswA

query
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active site: A66 (= A71), I71 (≠ F76), A84 (≠ I89), Q88 (= Q93), G112 (= G117), E115 (= E120), P136 (= P141), E137 (= E142), G145 (= G150), D264 (= D269), S422 (= S420), H443 (= H441), E455 (= E453), N493 (= N491)
binding coenzyme a: E23 (≠ S29), M25 (≠ V31), A66 (= A71), D67 (= D72), I68 (≠ L73), P136 (= P141), E137 (= E142), L140 (= L145), T290 (≠ E295), K293 (= K298)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
31% identity, 96% coverage: 18:670/682 of query aligns to 48:745/763 of P40939

query
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P40939

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L

D

T

T

Q

Q

M

L

V

E

S

A

K

G

G

H

F

R

L

G

G

K

R

K

K

T

S

G

G

Q

K

G

G

F

F

Y

Y

R

I

W

Y

E

Q

N

E

G

G

-

V

-

K

-

R

K

K

P

D

V

L

K

N

P

S

E

D

V

M

D

D

-

S

-

I

-

L

-

A

-

S

-

L

-

K

-

L

-

P

P

P

K

K

Y

S

V

E

A

V

P

S

A

S

-

D

-

E

D

D

L

I

E

Q

D

F

R

R

L

L

I

V

L

T

P

R

L

F

L

V

N

N

E

E

A

A

V

V

A

M

C

C

L

L

H

Q

D

E

R

G

V

I

V

L

D

A

D

T

A

P

D

A

L

E

L

G

D

D

A

I

G

G

V

A

I

V

F

F

G

G

T

L

G

G

F

F

A

P

P

P

F

C

R

L

G

G

G

G

P

P

I

F

A

R

H

F

I

V

R

D

A

L

V

Y

G

G

A

A

A

Q

T

K

L

I

E

V

A

D

R

R

L

L

G

K

A

K

L

Y

A

E

A

A

R

A

H

Y

G

G

P

K

R

Q

F

F

T

T

V282 (≠ L230) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
I305 (= I254) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
L342 (≠ V290) to P: in LCHAD deficiency; dbSNP:rs137852772
E510 (= E453) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
34% identity, 94% coverage: 19:661/682 of query aligns to 17:696/715 of 1wdlA

query
sites
1wdlA

I

I

A

V

T

E

L

L

T

K

L

F

D

D

R

L

A

K

G

G

S

E

P

S

V

V

N

N

A

K

L

F

S

N

R

R

A

L

V

T

L

L

D

N

E

E

F

L

D

R

Q

Q

I

A

V

V

E

D

R

A

L

I

S

K

F

A

E

D

P

A

P

S

-

V

R

K

G

G

L

V

V

I

I

V

R

S

S

S

G

G

K

K

S

D

A

V

G

-

F

F

V

I

V

V

G

G

A
|
A

D

D

L

I

K

T

E

E

F

F

A

V

G

E

Y

N

E

F

Q

K

-

L

-

P

G

D

G

A

S

E

V

L

I

I

D

A

Q

G

I
x
N

R

L

N

E

G

A

Q
x
N

A

K

V

I

F

F

E

S

R

D

L

F

A

E

R

D

L

L

R

N

C

V

P

P

T

T

A

V

V

A

A

A

I

I

H

N

G

G

H

I

C

A

M

L

G

G

G
|
G

G

G

T

L

E
|
E

L

M

S

C

L

L

A

A

C

A

N

D

Y

F

R

R

I

V

A

M

S

A

D

D

D

-

A

-

S

S

T

A

R

K

I

I

G

G

L

L

P
|
P

E
|
E

V

V

K

K

L

L

G

G

I

I

F

Y

P
|
P

G
|
G

W

F

G

G

G

G

S

T

A

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

A

V

P

D

K

N

A

A

L

V

E

E

F

W

M

I

L

A

T

S

G

G

R

K

T

E

A

N

S

R

A

A

A

E

S

D

A

A

R

L

A

K

L

V

G

S

L

A

V

V

D

D

A

A

I

V

A

V

T

T

P

A

E

D

R

K

L

L

-

G

-

A

-

A

-

A

L

L

D

D

V

L

A

I

K

K

E

R

W

A

V

I

R

S

R

G

R

E

P

L

A

D

P

Y

P

K

F

A

G

K

R

R

R

Q

A

P

L

K

A

L

W

E

A

K

T

L

N

K

L

L

W

N

P

A

V

I

R

E

Q

Q

A

M

L

M

A

A

P

F

V

E

L

T

R

A

K

K

Q

G

T

F

A

V

A

A

K

G

A

Q

R

A

P

G

E

P

H

N

Y

Y

P

P

A

A

P

P

F

V

A

E

L

A

I

I

E

K

T

T

W

I

R

Q

R

K

G

A

G

A

S

N

S

F

I

G

R

R

Q

D

R

K

-

A

L

L

D

E

L

V

E

E

A

A

R

A

A

G

V

F

A

A

K

K

L

L

A

A

Q

K

T

T

P

S

T

A

C

S

R

N

N

C

L

L

I

I

R

G

V

L

F

F

F

L

L

N

Q

D

E

Q

R

E

L

L

K

K

A

K

Q

K

G

A

A

K

-

V

-

Y

-

D

G

K

V

I

E

A

H

K

G

D

I

V

V

K

R

Q

I

A

H

A

V

V

V

L

G

G

A
|
A

G

G

V
x
I

M
|
M

G

G

G

G

D

G

I

I

A

A

A

Y

W

Q

A

S

A

A

L

S

R

K

G

G

F

T

E

P

V

I

T

L

L

M

Q

K

D
|
D

R
x
I

E

N

D

E

A

-

Y

-

V

-

R

-

P

H

A

G

I

I

E

E

R

Q

G

G

R

L

A

A

L

E

F

A

A

A

K

K

K

L

L

L

-

V

-

G

-

R

-

V

-

D

-

K

-

G

-

R

R

M

A

T

P

P

E

A

R

K

V

M

E

A

A

E

A

V

S

L

R

N

R

G

L

I

V

R

A

P

D

T

V

L

E

S

G

Y

A

G

G

D

V

F

A

G

E

N

A

V

D

D

L

L

V

V

I

V

E

E

A
|
A

I
x
V

F

V

E
|
E

N

N

L

P

E

K

A

V

K

K

Q

Q

A

A

L

V

Y

L

R

A

Q

E

L

V

E

E

P

N

R

H

M

V

K

R

P

E

G

D

A

A

L

I

L

L

A

A

S

S

N
|
N

T
|
T

S
|
S

S

T

I

I

P

S

L

I

D

S

E

L

L

L

R

A

A

K

A

A

V

L

A

K

T

R

P

P

G

E

R

N

F

F

L

V

G

G

L

M

H
|
H

Y

F

F

F

N

N

P

P

V

V

A

H

L

M

M

M

P

P

L

L

V

V

E
|
E

I

V

V

I

R

R

H

G

D

E

R

K

L

S

D

S

P

D

A

L

A

A

E

V

K

A

R

T

A

T

A

V

A

A

L

Y

C

A

K

K

A

K

L

M

D

G

K

K

L

N

P

P

I

I

A

V

V

V

A

N

G

D

T

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

M

F

P

P

Y

Y

L

F

L

G

E

G

A

F

I

A

R

K

I

L

L

V

K

S

E

A

G

G

V

V

P

D

A

F

P

V

V

R

I

I

D

D

K

K

V

V

A

M

R

E

K

K

F

F

G

G

M

W

P

P

M

M

G

G

P

P

I

A

E

Y

L

L

A

M

D

D

T

V

I

V

G

G

L

I

D

D

V

-

C

T

A

G

S

H

V

H

G

G

R

R

E

D

L

V

A

M

P

A

F

E

-

G

-

F

-

P

-

D

-

R

L

M

G

K

L

D

D

D

L

R

P

R

E

S

G

A

I

I

D

D

T

A

Q

L

L

Y

E

E

A

A

G

K

H

R

R

L

G

G

K

Q

K

K

T

N

G

G

Q

K

G

G

F

F

Y

Y

R

A

W

Y

E

E

N

A

G

D

K

K

P

K

-

G

-

K

-

Q

-

K

-

K

-

L

-

V

-

D

-

S

-

S

-

V

-

L

-

E

-

V

V

L

K

K

P

P

E

I

V

V

-

Y

D

E

P

Q

K

R

Y

D

V

V

A

T

P

D

A

E

D

D

L

I

E

I

D

N

R

W

L

M

I

M

L

I

P

P

L

L

L

C

N

L

E

E

A

T

V

V

A

R

C

C

L

L

H

E

D

D

R

G

V

I

V

V

D

E

D

T

A

A

D

A

L

E

L

A

D

D

A

M

G

G

V

L

I

V

F

Y

G

G

T

I

G

G

F

F

A

P

P

L

F

F

R

R

G

G

G

G

P

A

I

L

A

R

H

Y

I

I

R

D

A

S

V

I

G

G

A

V

A

A

T

E

L

F

-

V

-

A

-

L

-

A

-

D

-

Q

E

Y

A

A

R

E

L

L

G

G

A

A

L

L

active site: A69 (= A71), N89 (≠ I89), N93 (≠ Q93), G117 (= G117), E120 (= E120), P139 (= P141), E140 (= E142), P147 (= P149), G148 (= G150), S430 (= S420), H451 (= H441), E463 (= E453), N501 (= N491)
binding nicotinamide-adenine-dinucleotide: A322 (= A316), I324 (≠ V318), M325 (= M319), D344 (= D338), I345 (≠ R339), A400 (= A390), V401 (≠ I391), E403 (= E393), N428 (= N418), T429 (= T419), S430 (= S420)

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
34% identity, 94% coverage: 19:661/682 of query aligns to 17:696/715 of P28793

query
sites
P28793

I

I

A

V

T

E

L

L

T

K

L

F

D

D

R

L

A

K

G

G

S

E

P

S

V

V

N

N

A

K

L

F

S

N

R

R

A

L

V

T

L

L

D

N

E

E

F

L

D

R

Q

Q

I

A

V

V

E

D

R

A

L

I

S

K

F

A

E

D

P

A

P

S

-

V

R

K

G

G

L

V

V

I

I

V

R

S

S

S

G

G

K

K

S

D

A

V

G

-

F

F

V

I

V

V

G

G

A

A

D

D

L

I

K

T

E

E

F

F

A

V

G

E

Y

N

E

F

Q

K

-

L

-

P

G

D

G

A

S

E

V

L

I

I

D

A

Q

G

I

N

R

L

N

E

G

A

Q

N

A

K

V

I

F

F

E

S

R

D

L

F

A

E

R

D

L

L

R

N

C

V

P

P

T

T

A

V

V

A

A

A

I

I

H

N

G

G

H

I

C

A

M

L

G

G

G

G

G

G

T

L

E

E

L

M

S

C

L

L

A

A

C

A

N

D

Y

F

R

R

I

V

A

M

S

A

D

D

D

-

A

-

S

S

T

A

R

K

I

I

G

G

L

L

P

P

E

E

V

V

K

K

L

L

G

G

I

I

F

Y

P

P

G

G

W

F

G

G

G

G

S

T

A

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

A

V

P

D

K

N

A

A

L

V

E

E

F

W

M

I

L

A

T

S

G

G

R

K

T

E

A

N

S

R

A

A

A

E

S

D

A

A

R

L

A

K

L

V

G

S

L

A

V

V

D

D

A

A

I

V

A

V

T

T

P

A

E

D

R

K

L

L

-

G

-

A

-

A

-

A

L

L

D

D

V

L

A

I

K

K

E

R

W

A

V

I

R

S

R

G

R

E

P

L

A

D

P

Y

P

K

F

A

G

K

R

R

R

Q

A

P

L

K

A

L

W

E

A

K

T

L

N

K

L

L

W

N

P

A

V

I

R

E

Q

Q

A

M

L

M

A

A

P

F

V

E

L

T

R

A

K

K

Q

G

T

F

A

V

A

A

K

G

A

Q

R

A

P

G

E

P

H

N

Y

Y

P

P

A

A

P

P

F

V

A

E

L

A

I

I

E

K

T

T

W

I

R

Q

R

K

G

A

G

A

S

N

S

F

I

G

R

R

Q

D

R

K

-

A

L

L

D

E

L

V

E

E

A

A

R

A

A

G

V

F

A

A

K

K

L

L

A

A

Q

K

T

T

P

S

T

A

C

S

R

N

N

C

L

L

I

I

R

G

V

L

F

F

F

L

L

N

Q
x
D

E

Q

R

E

L

L

K

K

A

K

Q

K

G

A

A

K

-

V

-

Y

-

D

G

K

V

I

E

A

H

K

G

D

I

V

V

K

R

Q

I

A

H

A

V

V

V

L

G

G

A

A

G

G

V

I

M
|
M

G

G

G

G

D

G

I

I

A

A

A

Y

W

Q

A

S

A

A

L

S

R

K

G

G

F

T

E

P

V

I

T

L

L

M

Q

K

D
|
D

R

I

E

N

D

E

A

-

Y

-

V

-

R

-

P

H

A

G

I

I

E

E

R

Q

G

G

R

L

A

A

L

E

F

A

A

A

K

K

K

L

L

L

-

V

-

G

-

R

-

V

-

D

-

K

-

G

-

R

R

M

A

T

P

P

E

A

R

K

V

M

E

A

A

E

A

V

S

L

R

N

R

G

L

I

V

R

A

P

D

T

V

L

E

S

G

Y

A

G

G

D

V

F

A

G

E

N

A

V

D

D

L

L

V

V

I

V

E

E

A

A

I
x
V

F
x
V

E
|
E

N

N

L

P

E

K

A

V

K
|
K

Q

Q

A

A

L

V

Y

L

R

A

Q

E

L

V

E

E

P

N

R

H

M

V

K

R

P

E

G

D

A

A

L

I

L

L

A

A

S

S

N

N

T

T

S
|
S

S

T

I

I

P

S

L

I

D

S

E

L

L

L

R

A

A

K

A

A

V

L

A

K

T

R

P

P

G

E

R

N

F

F

L

V

G

G

L

M

H

H

Y

F

F

F

N
|
N

P

P

V

V

A

H

L

M

M

M

P

P

L

L

V

V

E

E

I

V

V

I

R

R

H

G

D

E

R

K

L

S

D

S

P

D

A

L

A

A

E

V

K

A

R

T

A

T

A

V

A

A

L

Y

C

A

K

K

A

K

L

M

D

G

K

K

L

N

P

P

I

I

A

V

V

V

A

N

G

D

T

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

M

F

P

P

Y

Y

L

F

L

G

E

G

A

F

I

A

R

K

I

L

L

V

K

S

E

A

G

G

V

V

P

D

A

F

P

V

V

R

I

I

D

D

K

K

V

V

A

M

R

E

K

K

F

F

G

G

M

W

P

P

M

M

G

G

P

P

I

A

E

Y

L

L

A

M

D

D

T

V

I

V

G

G

L

I

D

D

V

-

C

T

A

G

S

H

V

H

G

G

R

R

E

D

L

V

A

M

P

A

F

E

-

G

-

F

-

P

-

D

-

R

L

M

G

K

L

D

D

D

L

R

P

R

E

S

G

A

I

I

D

D

T

A

Q

L

L

Y

E

E

A

A

G

K

H

R

R

L

G

G

K

Q

K

K

T

N

G

G

Q

K

G

G

F

F

Y

Y

R

A

W

Y

E

E

N

A

G

D

K

K

P

K

-

G

-

K

-

Q

-

K

-

K

-

L

-

V

-

D

-

S

-

S

-

V

-

L

-

E

-

V

V

L

K

K

P

P

E

I

V

V

-

Y

D

E

P

Q

K

R

Y

D

V

V

A

T

P

D

A

E

D

D

L

I

E

I

D

N

R

W

L

M

I

M

L

I

P

P

L

L

L

C

N

L

E

E

A

T

V

V

A

R

C

C

L

L

H

E

D

D

R

G

V

I

V

V

D

E

D

T

A

A

D

A

L

E

L

A

D

D

A

M

G

G

V

L

I

V

F
x
Y

G

G

T

I

G

G

F

F

A

P

P

L

F

F

R

R

G

G

G

G

P

A

I

L

A

R

H

Y

I

I

R

D

A

S

V

I

G

G

A

V

A

A

T

E

L

F

-

V

-

A

-

L

-

A

-

D

-

Q

E

Y

A

A

R

E

L

L

G

G

A

A

L

L

D297 (≠ Q294) binding substrate
M325 (= M319) binding NAD(+)
D344 (= D338) binding NAD(+)
VVE 401:403 (≠ IFE 391:393) binding NAD(+)
K408 (= K398) binding NAD(+)
S430 (= S420) binding NAD(+)
N454 (= N444) binding NAD(+)
N501 (= N491) binding substrate
Y660 (≠ F631) binding substrate

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
34% identity, 94% coverage: 19:661/682 of query aligns to 17:688/707 of 1wdmA

query
sites
1wdmA

I

I

A

V

T

E

L

L

T

K

L

F

D

D

R

L

A

K

G

G

S

E

P

S

V

V

N

N

A

K

L

F

S

N

R

R

A

L

V

T

L

L

D

N

E

E

F

L

D

R

Q

Q

I

A

V

V

E

D

R

A

L

I

S

K

F

A

E

D

P

A

P

S

-

V

R

K

G

G

L

V

V

I

I

V

R

S

S

S

G

G

K

K

S

D

A

V

G

-

F

F

V

I

V

V

G

G

A
|
A

D

D

L

I

K

T

E

E

F

F

A

V

G

E

Y

N

E

F

Q

K

-

L

-

P

G

D

G

A

S

E

V

L

I

I

D

A

Q

G

I
x
N

R

L

N

E

G

A

Q
x
N

A

K

V

I

F

F

E

S

R

D

L

F

A

E

R

D

L

L

R

N

C

V

P

P

T

T

A

V

V

A

A

A

I

I

H

N

G

G

H

I

C

A

M

L

G

G

G
|
G

G

G

T

L

E
|
E

L

M

S

C

L

L

A

A

C

A

N

D

Y

F

R

R

I

V

A

M

S

A

D

D

D

-

A

-

S

S

T

A

R

K

I

I

G

G

L

L

P
|
P

E
|
E

V

V

K
|
K

L

L

G

G

I

I

F

Y

P
|
P

G
|
G

W

F

G

G

G

G

S

T

A

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

A

V

P

D

K

N

A

A

L

V

E

E

F

W

M

I

L

A

T

S

G

G

R

K

T

E

A

N

S

R

A

A

A

E

S

D

A

A

R

L

A

K

L

V

G

S

L

A

V

V

D

D

A

A

I

V

A

V

T

T

P

A

E

D

R

K

L

L

-

G

-

A

-

A

-

A

L

L

D

D

V

L

A

I

K

K

E

R

W

A

V

I

R

S

R

G

R

E

P

L

A

D

P

Y

P

K

F

A

G

K

R

R

R

Q

A

P

L

K

A

L

W

E

A

K

T

L

N

K

L

L

W

N

P

A

V

I

R

E

Q

Q

A

M

L

M

A

A

P

F

V

E

L

T

R

A

K

K

Q

G

T

F

A

V

A

A

K

G

A

Q

R

A

P

G

E

P

H

N

Y

Y

P

P

A

A

P

P

F

V

A

E

L

A

I

I

E

K

T

T

W

I

R

Q

R

K

G

A

G

A

S

N

S

F

I

G

R

R

Q

D

R

K

-

A

L

L

D

E

L

V

E

E

A

A

R

A

A

G

V

F

A

A

K

K

L

L

A

A

Q

K

T

T

P

S

T

A

C

S

R

N

N

C

L

L

I

I

R

G

V

L

F

F

F

L

L

N

Q
x
D

E

Q

R

E

L

L

K

K

A

K

Q

K

G

A

A

K

-

V

-

Y

-

D

G

K

V

I

E

A

H

K

G

D

I

V

V

K

R

Q

I

A

H

A

V

V

V

L

G
|
G

A
|
A

G

G

V
x
I

M
|
M

G

G

G

G

D

G

I

I

A

A

A

Y

W

Q

A

S

A

A

L

S

R

K

G

G

F

T

E

P

V

I

T

L

L

M

Q

K

D
|
D

R

I

E

N

D

E

A

-

Y

-

V

-

R

-

P

H

A

G

I

I

E

E

R

Q

G

G

R

L

A

A

L

E

F

A

A

A

K

K

K

L

L

L

-

V

-

G

-

R

-

V

-

D

-

K

-

G

-

R

R

M

A

T

P

P

E

A

R

K

V

M

E

A

A

E

A

V

S

L

R

N

R

G

L

I

V

R

A

P

D

T

V

L

E

S

G

Y

A

G

G

D

V

F

A

G

E

N

A

V

D

D

L

L

V

V

I

V

E

E

A

A

I
x
V

F

V

E
|
E

N

N

L

P

E

K

A

V

K

K

Q

Q

A

A

L

V

Y

L

R

A

Q

E

L

V

E

E

P

N

R

H

M

V

K

R

P

E

G

D

A

A

L

I

L

L

A

A

S

S

N
|
N

T

T

S
|
S

S

T

I

I

P

S

L

I

D

S

E

L

L

L

R

A

A

K

A

A

V

L

A

K

T

R

P

P

G

E

R

N

F

F

L

V

G

G

L

M

H
|
H

Y

F

F

F

N
|
N

P

P

V

V

A

H

L

M

M
|
M

P

P

L

L

V

V

E
|
E

I

V

V

I

R

R

H

G

D

E

R

K

L

S

D

S

P

D

A

L

A

A

E

V

K

A

R

T

A

T

A

V

A

A

L

Y

C

A

K

K

A

K

L

M

D

G

K

K

L

N

P

P

I

I

A

V

V

V

A

N

G

D

T

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

M

F

P

P

Y

Y

L

F

L

G

E

G

A

F

I

A

R

K

I

L

L

V

K

S

E

A

G

G

V

V

P

D

A

F

P

V

V

R

I

I

D

D

K

K

V

V

A

M

R

E

K

K

F

F

G

G

M

W

P
|
P

M

M

G

G

P

P

I

A

E

Y

L

L

A

M

D

D

T

V

I

V

G

G

L

I

D

D

V

-

C

T

A

G

S

H

V

H

G

G

R

R

E

D

L

V

A

M

P

A

F

E

-

G

-

F

-

P

-

D

-

R

L

M

G

K

L

D

D

D

L

R

P

R

E

S

G

A

I

I

D

D

T

A

Q

L

L

Y

E

E

A

A

G

K

H

R

R

L

G

G

K

Q

K

K

T

N

G

G

Q

K

G

G

F

F

Y

Y

R

A

W

Y

E

K

N

K

-

L

-

V

-

D

-

S

-

S

-

V

G

L

K

E

P

V

V

L

K

K

P

P

E

I

V

V

-

Y

D

E

P

Q

K

R

Y

D

V

V

A

T

P

D

A

E

D

D

L

I

E

I

D

N

R

W

L

M

I

M

L

I

P

P

L

L

L

C

N

L

E

E

A

T

V

V

A

R

C

C

L

L

H

E

D

D

R

G

V

I

V

V

D

E

D

T

A

A

D

A

L

E

L

A

D

D

A

M

G

G

V

L

I

V

F
x
Y

G

G

T

I

G

G

F

F

A

P

P
x
L

F

F

R

R

G

G

G

G

P

A

I

L

A

R

H

Y

I

I

R

D

A

S

V

I

G

G

A

V

A

A

T

E

L

F

-

V

-

A

-

L

-

A

-

D

-

Q

E

Y

A

A

R

E

L

L

G

G

A

A

L

L

active site: A69 (= A71), N89 (≠ I89), N93 (≠ Q93), G117 (= G117), E120 (= E120), P139 (= P141), E140 (= E142), P147 (= P149), G148 (= G150), S430 (= S420), H451 (= H441), E463 (= E453), N501 (= N491)
binding acetyl coenzyme *a: K142 (= K144), D297 (≠ Q294), M459 (= M449), N501 (= N491), P534 (= P524), Y652 (≠ F631), L658 (≠ P637)
binding nicotinamide-adenine-dinucleotide: G321 (= G315), A322 (= A316), I324 (≠ V318), M325 (= M319), D344 (= D338), V401 (≠ I391), E403 (= E393), N428 (= N418), S430 (= S420), N454 (= N444)

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
33% identity, 93% coverage: 19:652/682 of query aligns to 17:681/729 of P21177

query
sites
P21177

I

I

A

A

T

E

L

L

T

V

L

F

D

D

R

A

A

P

G

G

S

S

P

-

V

V

N

N

A

K

L

L

S

D

R

T

A

A

V

T

L

V

D

A

E

S

F

L

D

G

Q

E

I

A

V

I

E

G

R

V

L

L

S

E

F

Q

E

Q

P

S

P

D

-

L

R

K

G

G

L

L

V

L

I

L

R

R

S

S

G

N

K

K

S

A

A

A

G

-

F

F

V

I

V

V

G

G

A

A

D

D

L

I

K

T

E

E

F

F

A

L

G

S

Y

L

-

F

-

L

-

V

-

P

-

E

E

E

Q

Q

G

-

G

-

S

-

V

L

I

S

D

Q

Q

W

I

L

R

H

N

F

G

A

Q

N

A

S

V

V

F

F

E

N

R

R

L

L

A

E

R

D

L

L

R

P

C

V

P

P

T

T

A

I

V

A

A

A

I

V

H

N

G

G

H

Y

C

A

M

L

G

G

G
|
G

G

G

T

C

E

E

L

C

S

V

L

L

A

A

C

T

N

D

Y

Y

R

R

I

L

A

A

S

T

D

P

D

D

A

-

S

-

T

L

R

R

I

I

G

G

L

L

P

P

E

E

V

T

K

K

L

L

G

G

I

I

F

M

P

P

G

G

W

F

G

G

G

G

S

S

A

V

R

R

L

M

P

P

R

R

L

M

I

L

G

G

A

A

P

D

K

S

A

A

L

L

E

E

F

I

M

I

L

A

T

A

G

G

R

K

T

D

A

V

S

G

A

A

A

D

S

Q

A

A

R

L

A

K

L

I

G

G

L

L

V

V

D

D

A

G

I

V

A

V

T

K

P

A

E

E

R

K

L

L

L

V

D

E

V

G

A

A

K

K

E

A

W

V

V

L

R

R

R

Q

R

A

P

I

A

N

P

G

P

D

F

L

G

D

-

W

-

K

-

A

-

K

R

R

R

Q

A

P

L

K

A

L

W

E

A

P

T

L

N

K

L

L

W

S

P

K

V

I

R

E

Q

A

A

T

L

M

A

S

P

F

V

T

L

I

R

A

K

K

Q

G

T

M

A

V

A

A

K

Q

A

T

R

A

P

G

E

K

H

H

Y

Y

P

P

A

A

P

P

F

I

A

T

L

A

I

V

E

K

T

T

W

I

R

E

R

A

G

A

G

A

S

R

S

F

I

G

R

R

Q

E

R

E

-

A

L

L

D

N

L

L

E

E

A

N

R

K

A

S

V

F

A

V

K

P

L

L

A

A

Q

H

T

T

P

N

T

E

C

A

R

R

N

A

L

L

I

V

R

G

V

I

F

F

F

L

L

N

Q

D

E

Q

R

Y

L

V

K

K

A

G

Q

K

G

A

A

K

G

K

V

L

E

T

H

K

G

D

I

V

-

E

-

T

-

P

V

K

R

Q

I

A

H

A

V

V

V

L

G

G

A

A

G
|
G

V

I

M

M

G

G

G

G

D

G

I

I

A

A

A

Y

W

Q

A

S

A

A

L

W

R

K

G

G

F

V

E

P

V

V

T

V

L

M

Q

K

D

D

R

I

E

N

D

D

A

K

Y

S

V

L

R

T

P

L

A

G

I

M

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A

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A

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K

L

L

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N

K

K

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L

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A

G116 (= G117) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
G322 (= G317) mutation to A: 10-fold increase in KM for NADH.
H450 (= H441) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
33% identity, 93% coverage: 19:652/682 of query aligns to 17:681/719 of 6tnmA

query
sites
6tnmA

I

I

A

A

T

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L

L

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L

F

D

D

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A

A

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