SitesBLAST
Comparing WP_067643618.1 NCBI__GCF_001632775.1:WP_067643618.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3ozbA Crystal structure of 5'-methylthioinosine phosphorylase from psedomonas aeruginosa in complex with hypoxanthine (see paper)
52% identity, 97% coverage: 8:248/249 of query aligns to 5:241/241 of 3ozbA
- active site: T10 (= T13), P33 (= P36), F56 (≠ V62), P57 (≠ A63), A83 (= A89), M182 (= M188), T183 (= T189), N206 (= N212), A208 (= A214), M218 (≠ L225)
- binding hypoxanthine: A83 (= A89), V84 (= V90), G85 (= G91), L163 (= L169), E164 (= E170), V180 (= V186), G181 (= G187), M182 (= M188), N206 (= N212)
Q8U2I1 6-oxopurine nucleoside phosphorylase; Purine nucleoside phosphorylase; PNP; PfPNP; EC 2.4.2.1 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 2 papers)
44% identity, 96% coverage: 7:246/249 of query aligns to 4:245/265 of Q8U2I1
- C136 (≠ S137) modified: Disulfide link with 202
- C162 (≠ A163) modified: Disulfide link with 190
- E169 (= E170) mutation to S: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with D-211, D-213 and A-223.
- C190 (≠ M191) modified: Disulfide link with 162
- C202 (≠ D203) modified: Disulfide link with 136
- N211 (= N212) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-213 and A-223.
- A213 (= A214) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and A-223.
- H223 (≠ L225) mutation to A: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and D-213.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 254 modified: Disulfide link with 256; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-256.
- 256 modified: Disulfide link with 254; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-254.
3t94A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (mtap) ii complexed with 5'-deoxy-5'-methylthioadenosine and sulfate (see paper)
43% identity, 83% coverage: 7:213/249 of query aligns to 10:215/270 of 3t94A
- active site: S16 (≠ T13), P39 (= P36), H63 (= H60), I65 (≠ V62), P66 (≠ A63), A92 (= A89), M190 (= M188), T191 (= T189), D214 (≠ N212)
- binding 5'-deoxy-5'-methylthioadenosine: G94 (= G91), F170 (≠ L169), I188 (≠ V186), M190 (= M188), D214 (≠ N212)
Sites not aligning to the query:
Q97W94 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPII; EC 2.4.2.28 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
43% identity, 83% coverage: 7:213/249 of query aligns to 10:215/270 of Q97W94
- C138 (≠ S137) modified: Disulfide link with 205
- C200 (≠ R198) modified: Disulfide link with 262
- C205 (≠ D203) modified: Disulfide link with 138
Sites not aligning to the query:
- 259 modified: Disulfide link with 261; C→S: Reduces thermostability of the enzyme; when associated with S-261.
- 261 modified: Disulfide link with 259; C→S: Reduces thermostability of the enzyme; when associated with S-259.
- 262 modified: Disulfide link with 200; C→S: Reduces thermostability of the enzyme.
4gljA Crystal structure of methylthioadenosine phosphorylase in complex with rhodamine b (see paper)
41% identity, 88% coverage: 6:224/249 of query aligns to 5:224/287 of 4gljA
- active site: D212 (≠ N212), D214 (≠ A214)
- binding N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium: S12 (≠ T13), Y15 (= Y16), H55 (= H56), A88 (= A89), G90 (= G91), F169 (≠ L169), F169 (≠ L169), I186 (≠ V186), G187 (= G187), M188 (= M188), D212 (≠ N212), F213 (≠ W213), D214 (≠ A214), N219 (≠ D219)
Sites not aligning to the query:
9jd2A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase from aeropyrum pernix complex with 5'-deoxy-5'-methylthioadenosine 353k
44% identity, 83% coverage: 7:213/249 of query aligns to 12:218/273 of 9jd2A
Sites not aligning to the query:
1wtaA Crystal structure of 5'-deoxy-5'-methylthioadenosine from aeropyrum pernix (r32 form)
44% identity, 83% coverage: 7:213/249 of query aligns to 12:218/273 of 1wtaA
Sites not aligning to the query:
Q8U4Q8 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; PfMTAP; EC 2.4.2.28 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
40% identity, 87% coverage: 7:223/249 of query aligns to 4:218/257 of Q8U4Q8
- C130 (≠ S137) modified: Disulfide link with 195
- C195 (≠ D203) modified: Disulfide link with 130
Sites not aligning to the query:
- 246 modified: Disulfide link with 248
- 248 modified: Disulfide link with 246
5eubA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with 2-amino-mta and sulfate
33% identity, 97% coverage: 5:246/249 of query aligns to 1:246/272 of 5eubA
- active site: T9 (= T13), P32 (= P36), H56 (= H60), I58 (≠ V62), M59 (≠ A63), A85 (= A89), M187 (= M188), T188 (= T189), D211 (≠ N212), D213 (≠ A214), V224 (≠ L225)
- binding (2~{R},3~{R},4~{S},5~{S})-2-[2,6-bis(azanyl)purin-9-yl]-5-(methylsulfanylmethyl)oxolane-3,4-diol: A85 (= A89), C86 (≠ V90), G87 (= G91), F168 (≠ L169), I185 (≠ V186), N186 (≠ G187), T210 (≠ A211), D211 (≠ N212), D213 (≠ A214), V227 (≠ I228)
6dz0A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((pent-4-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
33% identity, 97% coverage: 5:246/249 of query aligns to 4:249/274 of 6dz0A
- active site: T12 (= T13), P35 (= P36), H59 (= H60), I61 (≠ V62), M62 (≠ A63), A88 (= A89), M190 (= M188), T191 (= T189), D214 (≠ N212), D216 (≠ A214), V227 (≠ L225)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(pent-4-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T12 (= T13), H59 (= H60), A88 (= A89), C89 (≠ V90), G90 (= G91), F171 (≠ L169), I188 (≠ V186), N189 (≠ G187), M190 (= M188), T213 (≠ A211), D214 (≠ N212), D216 (≠ A214), V227 (≠ L225), V230 (≠ I228)
- binding phosphate ion: G11 (= G12), T12 (= T13), R54 (= R55), H55 (= H56), T87 (≠ N88), T191 (= T189)
5tc5A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with butylthio-dadme-immucillin-a and chloride
33% identity, 98% coverage: 2:246/249 of query aligns to 12:260/286 of 5tc5A
- active site: T23 (= T13), P46 (= P36), H70 (= H60), I72 (≠ V62), M73 (≠ A63), A99 (= A89), M201 (= M188), T202 (= T189), D225 (≠ N212), D227 (≠ A214), V238 (≠ L225)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol: A99 (= A89), C100 (≠ V90), G101 (= G91), F182 (≠ L169), I199 (≠ V186), M201 (= M188), D225 (≠ N212), D227 (≠ A214), V241 (≠ I228)
6dz3A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-(((3-(1-butyl-1h-1,2,3-triazol-4-yl) propyl)thio)methyl)pyrrolidin-3-ol (see paper)
33% identity, 97% coverage: 5:246/249 of query aligns to 2:247/273 of 6dz3A
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M188 (= M188), T189 (= T189), D212 (≠ N212), D214 (≠ A214), V225 (≠ L225)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-({[3-(1-butyl-1H-1,2,3-triazol-4-yl)propyl]sulfanyl}methyl)pyrrolidin-3-ol: T84 (≠ V87), A86 (= A89), C87 (≠ V90), G88 (= G91), F169 (≠ L169), I186 (≠ V186), N187 (≠ G187), M188 (= M188), D212 (≠ N212), D214 (≠ A214), V228 (≠ I228), L232 (= L232)
6dyzA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((prop-2-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
33% identity, 97% coverage: 5:246/249 of query aligns to 2:247/273 of 6dyzA
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M188 (= M188), T189 (= T189), D212 (≠ N212), D214 (≠ A214), V225 (≠ L225)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(prop-2-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T10 (= T13), H57 (= H60), A86 (= A89), C87 (≠ V90), G88 (= G91), F169 (≠ L169), I186 (≠ V186), N187 (≠ G187), M188 (= M188), D212 (≠ N212), D214 (≠ A214), V228 (≠ I228)
- binding phosphate ion: G9 (= G12), T10 (= T13), R52 (= R55), H53 (= H56), T85 (≠ N88), A86 (= A89), T189 (= T189)
5tc8A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with methylthio-dadme-immucillin-a
33% identity, 97% coverage: 5:246/249 of query aligns to 2:247/273 of 5tc8A
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M188 (= M188), T189 (= T189), D212 (≠ N212), D214 (≠ A214), V225 (≠ L225)
- binding (3r,4s)-1-[(4-amino-5h-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol: T10 (= T13), A86 (= A89), C87 (≠ V90), G88 (= G91), F169 (≠ L169), I186 (≠ V186), N187 (≠ G187), M188 (= M188), T211 (≠ A211), D212 (≠ N212), D214 (≠ A214), V228 (≠ I228)
5tc6A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with propylthio-immucillin-a
33% identity, 97% coverage: 5:246/249 of query aligns to 2:247/273 of 5tc6A
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M188 (= M188), T189 (= T189), D212 (≠ N212), D214 (≠ A214), V225 (≠ L225)
- binding (2S,3S,4R,5S)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(propylsulfanyl)methyl]pyrrolidine-3,4-diol: T10 (= T13), A86 (= A89), C87 (≠ V90), G88 (= G91), F169 (≠ L169), N187 (≠ G187), M188 (= M188), D212 (≠ N212), V225 (≠ L225), V228 (≠ I228), L229 (≠ F229)
3ozcA Crystal structure of human 5'-deoxy-5'-methyladenosine phosphorylase in complex with pcl-phenylthiodadmeimma
33% identity, 97% coverage: 5:246/249 of query aligns to 2:247/273 of 3ozcA
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M188 (= M188), T189 (= T189), D212 (≠ N212), D214 (≠ A214), V225 (≠ L225)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(4-chlorophenyl)sulfanyl]methyl}pyrrolidin-3-ol: T10 (= T13), H57 (= H60), A86 (= A89), C87 (≠ V90), G88 (= G91), F169 (≠ L169), I186 (≠ V186), N187 (≠ G187), M188 (= M188), D212 (≠ N212), D214 (≠ A214), V228 (≠ I228), L229 (≠ F229)
1sd2A Structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase complexed with 5'-methylthiotubercidin (see paper)
36% identity, 84% coverage: 5:213/249 of query aligns to 2:207/262 of 1sd2A
- active site: T10 (= T13), P33 (= P36), H57 (= H60), I59 (≠ V62), M60 (≠ A63), A86 (= A89), M182 (= M188), T183 (= T189), D206 (≠ N212)
- binding 2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-methylsulfanylmethyl-tetrahydro-furan-3,4-diol: C87 (≠ V90), G88 (= G91), F163 (≠ L169), I180 (≠ V186), N181 (≠ G187), M182 (= M188), D206 (≠ N212)
Sites not aligning to the query:
1k27A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with a transition state analogue (see paper)
36% identity, 84% coverage: 5:213/249 of query aligns to 2:213/270 of 1k27A