SitesBLAST
Comparing WP_067650170.1 NCBI__GCF_001632775.1:WP_067650170.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7caxD Crystal structure of bacterial reductase
63% identity, 98% coverage: 4:242/243 of query aligns to 2:238/238 of 7caxD
- active site: G12 (= G14), S139 (= S141), Y152 (= Y154)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (= S12), R11 (= R13), I13 (= I15), H31 (= H33), R33 (= R35), S34 (= S36), R35 (= R37), D59 (= D61), V60 (= V62), N86 (= N88), A87 (= A89), G88 (= G90), S139 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), G183 (= G185), I185 (= I187)
4iiuA Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from escherichia coli strain cft073 complexed with NADP+ at 2.1 a resolution
56% identity, 99% coverage: 3:242/243 of query aligns to 2:243/243 of 4iiuA
- active site: G13 (= G14), S140 (= S141), Y153 (= Y154), K157 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), S11 (= S12), K12 (≠ R13), G13 (= G14), I14 (= I15), H32 (= H33), H34 (≠ R35), D36 (≠ R37), D60 (= D61), V61 (= V62), N87 (= N88), A88 (= A89), G89 (= G90), I90 (= I91), S140 (= S141), Y153 (= Y154), K157 (= K158), P183 (= P184), G184 (= G185), I186 (= I187), T188 (= T189), M190 (= M191), I191 (≠ V192)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
44% identity, 98% coverage: 4:241/243 of query aligns to 5:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
44% identity, 98% coverage: 4:241/243 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G14), S138 (= S141), Q148 (= Q151), Y151 (= Y154), K155 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (= S12), R11 (= R13), I13 (= I15), N31 (≠ H33), Y32 (≠ C34), A33 (≠ R35), G34 (≠ S36), S35 (≠ R37), A58 (≠ F60), N59 (≠ D61), V60 (= V62), N86 (= N88), A87 (= A89), T109 (= T111), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
43% identity, 98% coverage: 4:241/243 of query aligns to 6:241/244 of 6t77A
- active site: G16 (= G14), S138 (= S141), Y151 (= Y154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G10), S14 (= S12), R15 (= R13), T37 (≠ R35), L58 (≠ F60), N59 (≠ D61), V60 (= V62), A87 (= A89), G88 (= G90), I89 (= I91)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
42% identity, 98% coverage: 4:241/243 of query aligns to 9:240/243 of 4i08A
- active site: G19 (= G14), N113 (= N112), S141 (= S141), Q151 (= Q151), Y154 (= Y154), K158 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G10), S17 (= S12), R18 (= R13), I20 (= I15), T40 (≠ R35), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), G140 (≠ A140), S141 (= S141), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), T189 (= T189)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
42% identity, 98% coverage: 4:241/243 of query aligns to 9:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G10), S17 (= S12), R18 (= R13), I20 (= I15), T40 (≠ R35), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), I92 (= I91), V139 (≠ L139), S141 (= S141), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), I187 (= I187), T189 (= T189), M191 (= M191)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
43% identity, 98% coverage: 4:241/243 of query aligns to 6:241/244 of P0AEK2
- GASR 12:15 (≠ GSSR 10:13) binding NADP(+)
- T37 (≠ R35) binding NADP(+)
- NV 59:60 (≠ DV 61:62) binding NADP(+)
- N86 (= N88) binding NADP(+)
- Y151 (= Y154) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSAAK 154:158) binding NADP(+)
- A154 (= A157) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K158) mutation to A: Defect in the affinity for NADPH.
- I184 (= I187) binding NADP(+)
- E233 (≠ Q233) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
43% identity, 98% coverage: 4:241/243 of query aligns to 5:240/243 of 1q7bA
- active site: G15 (= G14), E101 (≠ D104), S137 (= S141), Q147 (= Q151), Y150 (= Y154), K154 (= K158)
- binding calcium ion: E232 (≠ Q233), T233 (≠ V234)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), S13 (= S12), R14 (= R13), T36 (≠ R35), N58 (≠ D61), V59 (= V62), N85 (= N88), A86 (= A89), G87 (= G90), I88 (= I91), S137 (= S141), Y150 (= Y154), K154 (= K158), P180 (= P184), G181 (= G185), I183 (= I187)
4ag3A Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with NADPH at 1.8a resolution (see paper)
44% identity, 99% coverage: 1:241/243 of query aligns to 10:251/254 of 4ag3A
- active site: G23 (= G14), S148 (= S141), Y161 (= Y154), K165 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G10), S21 (= S12), R22 (= R13), G23 (= G14), I24 (= I15), T44 (≠ S36), L68 (≠ F60), D69 (= D61), V70 (= V62), N96 (= N88), A97 (= A89), I146 (≠ L139), S148 (= S141), Y161 (= Y154), K165 (= K158), P191 (= P184), G192 (= G185), F193 (≠ L186), I194 (= I187), T196 (= T189), M198 (= M191), T199 (≠ V192)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
43% identity, 98% coverage: 4:241/243 of query aligns to 5:240/243 of 1q7cA
- active site: G15 (= G14), S137 (= S141), Q147 (= Q151), F150 (≠ Y154), K154 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), S13 (= S12), R14 (= R13), A35 (≠ C34), T36 (≠ R35), L57 (≠ F60), N58 (≠ D61), V59 (= V62), G87 (= G90), I88 (= I91)
4bo4C Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with n-(2-methoxyphenyl)-3,4- dihydro-2h-quinoline-1-carboxamide at 2.7a resolution (see paper)
44% identity, 99% coverage: 1:241/243 of query aligns to 16:252/255 of 4bo4C
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
46% identity, 100% coverage: 1:242/243 of query aligns to 3:238/240 of 4dmmB
- active site: G16 (= G14), S142 (= S141), Q152 (= Q151), Y155 (= Y154), K159 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G10), S14 (= S12), R15 (= R13), G16 (= G14), I17 (= I15), A37 (≠ R35), S38 (= S36), S39 (≠ R37), A62 (≠ F60), D63 (= D61), V64 (= V62), N90 (= N88), A91 (= A89), L113 (≠ T111), I140 (≠ L139), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), I188 (= I187), T190 (= T189), M192 (= M191)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
41% identity, 98% coverage: 4:241/243 of query aligns to 6:241/244 of P0A2C9
- M125 (≠ V128) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (≠ M223) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (≠ G224) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
9clyB Crystal structure of the 3-ketoacyl-(acyl-carrier-protein) reductase, cylg, from streptococcus agalactiae 2603v/r
39% identity, 98% coverage: 4:242/243 of query aligns to 4:240/242 of 9clyB
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), R13 (= R13), G14 (= G14), I15 (= I15), Y34 (≠ C34), N35 (≠ R35), S36 (= S36), C56 (≠ F60), N57 (≠ D61), I58 (≠ V62), N84 (= N88), I135 (≠ L139), S137 (= S141), Y150 (= Y154), K154 (= K158), G181 (= G185), I183 (= I187), T185 (= T189), M187 (= M191)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
43% identity, 100% coverage: 1:242/243 of query aligns to 4:245/247 of P73574
- A14 (≠ S11) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ R149) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K158) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ L186) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ V196) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
1uznA Maba from mycobacterium tuberculosis (see paper)
41% identity, 99% coverage: 1:241/243 of query aligns to 5:235/239 of 1uznA
- active site: G18 (= G14), S132 (= S141), Y145 (= Y154), K149 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), N16 (≠ S12), R17 (= R13), I19 (= I15), R39 (= R35), D53 (= D61), V54 (= V62), A81 (= A89), G82 (= G90)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
42% identity, 97% coverage: 6:241/243 of query aligns to 4:242/244 of 1edoA
- active site: G12 (= G14), S138 (= S141), Y151 (= Y154), K155 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (= S12), R11 (= R13), I13 (= I15), N31 (≠ H33), Y32 (≠ C34), A33 (≠ R35), R34 (≠ S36), S35 (≠ R37), D59 (= D61), V60 (= V62), N86 (= N88), A87 (= A89), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), I184 (= I187), S186 (≠ T189), M188 (= M191)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
41% identity, 98% coverage: 4:241/243 of query aligns to 5:240/243 of 7emgB
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 98% coverage: 4:242/243 of query aligns to 6:246/247 of 4jroC
- active site: G16 (= G14), S142 (= S141), Q152 (= Q151), Y155 (= Y154), K159 (= K158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G10), S14 (= S12), R15 (= R13), G16 (= G14), I17 (= I15), N35 (≠ H33), Y36 (≠ C34), N37 (≠ R35), G38 (≠ S36), S39 (≠ R37), N63 (≠ D61), V64 (= V62), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ T111), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), I188 (= I187), T190 (= T189)
Query Sequence
>WP_067650170.1 NCBI__GCF_001632775.1:WP_067650170.1
MTDKTILVTGSSRGIGKAIALRLARDGYDLVLHCRSRLDEATAVAADIAALGRGVRVLQF
DVGDRAAAAAALLADVEAHGCPYGVVCNAGIARDNAFPAMPPEDWDAVVHTNLDGFYNVL
HPLTMPLVRRRKPGRIVTLASVSGIIGNRGQVNYSAAKAGIIGATKALAVELASRAITVN
CVAPGLIDTEMVDARVLDEALKAIPAGRVGKPAEVAALVSFLMGEDAAYITRQVISVNGG
LVG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory