SitesBLAST
Comparing WP_068004602.1 NCBI__GCF_001623255.1:WP_068004602.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
49% identity, 97% coverage: 17:755/760 of query aligns to 7:750/759 of P76558
- K56 (≠ R66) modified: N6-acetyllysine
6zngF Maeb full-length acetyl-coa bound state (see paper)
48% identity, 95% coverage: 16:739/760 of query aligns to 5:723/753 of 6zngF
- active site: Y38 (= Y49), A74 (= A85), K93 (= K104), E135 (= E146), D136 (= D147), D160 (= D171), D161 (= D172), N286 (= N297)
- binding acetyl coenzyme *a: R511 (≠ L526), K514 (= K529), Y552 (= Y568), A553 (≠ S569), R557 (≠ E573), L560 (≠ S576), P571 (≠ V587), T590 (≠ A606), V591 (= V607), N592 (≠ H608), L593 (≠ D609), Y625 (= Y641), Q659 (≠ G676), L690 (≠ F706), N694 (≠ S710)
Sites not aligning to the query:
6zn4A Maeb malic enzyme domain apoprotein (see paper)
62% identity, 53% coverage: 16:417/760 of query aligns to 4:405/406 of 6zn4A
6zn7A Maeb malic enzyme domain apoprotein (see paper)
62% identity, 53% coverage: 16:417/760 of query aligns to 4:405/405 of 6zn7A
- active site: Y37 (= Y49), A73 (= A85), K92 (= K104), E134 (= E146), D135 (= D147), D159 (= D171), D160 (= D172), N285 (= N297)
- binding magnesium ion: E134 (= E146), D135 (= D147), D160 (= D172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T176), N191 (= N203), A193 (= A205), G194 (= G206), A195 (= A207), S196 (≠ A208), D218 (= D230), S219 (≠ T231), K235 (= K247), L260 (≠ V272), S261 (= S273), V262 (= V274), M283 (= M295), N285 (= N297), V315 (= V327)
5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
48% identity, 49% coverage: 44:416/760 of query aligns to 29:387/387 of 5ceeA
- active site: Y34 (= Y49), A70 (= A85), K89 (= K104), E131 (= E146), D132 (= D147), D156 (= D171), D157 (= D172), N283 (= N297)
- binding magnesium ion: E131 (= E146), D132 (= D147), D157 (= D172)
- binding nicotinamide-adenine-dinucleotide: T161 (= T176), N188 (= N203), G189 (= G204), G191 (= G206), A193 (= A208), D213 (= D230), K214 (≠ T231), V258 (= V272), S259 (= S273), I263 (≠ A277), L281 (≠ M295), N283 (= N297), V312 (= V327), N314 (= N329)
2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
51% identity, 45% coverage: 29:367/760 of query aligns to 18:354/383 of 2a9fA
2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
45% identity, 53% coverage: 17:416/760 of query aligns to 3:398/438 of 2dvmA
- active site: Y37 (= Y49), R73 (≠ A85), K92 (= K104), E134 (= E146), D135 (= D147), D159 (= D171), D160 (= D172), N296 (= N297)
- binding nicotinamide-adenine-dinucleotide: T164 (= T176), G194 (= G206), A195 (= A207), A196 (= A208), V217 (≠ C229), E218 (≠ D230), L219 (≠ I235), P224 (≠ E240), F269 (= F270), T270 (≠ G271), L294 (≠ M295), N296 (= N297), N327 (= N329)
2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 52% coverage: 18:409/760 of query aligns to 1:373/373 of 2haeD
2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
44% identity, 52% coverage: 18:409/760 of query aligns to 1:373/373 of 2haeB
- active site: Y31 (= Y49), A67 (= A85), K86 (= K104), E128 (= E146), D129 (= D147), D153 (= D171), D154 (= D172), N280 (= N297)
- binding nicotinamide-adenine-dinucleotide: T158 (= T176), N185 (= N203), G188 (= G206), A189 (= A207), A190 (= A208), D210 (= D230), R211 (≠ T231), V255 (= V272), S256 (= S273), R257 (≠ V274), L278 (≠ M295), A279 (= A296), N280 (= N297), N311 (= N329)
6ioxA Crystal structure of porphyromonas gingivalis phosphotransacetylase in complex with acetyl-coa (see paper)
34% identity, 42% coverage: 440:756/760 of query aligns to 8:331/339 of 6ioxA
1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
30% identity, 42% coverage: 436:756/760 of query aligns to 3:321/325 of 1xcoD
Q8ZND6 Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.222; EC 2.3.1.8 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 41% coverage: 448:756/760 of query aligns to 400:706/714 of Q8ZND6
Sites not aligning to the query:
- 252 R→H: Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH.
- 273 G→D: Increases speed of forward and back reactions by 2-3 fold.
- 294 M→I: Slightly decreases speed of forward and back reactions.
2af3C Phosphotransacetylase from methanosarcina thermophila soaked with coenzyme a (see paper)
27% identity, 41% coverage: 440:754/760 of query aligns to 4:322/332 of 2af3C
- binding coenzyme a: R86 (≠ L526), S127 (≠ Y568), L131 (= L572), V135 (≠ S576), L146 (≠ V587), A147 (≠ I588), G172 (≠ A606), M173 (≠ V607), M173 (≠ V607), V174 (≠ H608), E175 (≠ D609), N278 (≠ S710), Y281 (≠ T713), K282 (= K714), Q285 (≠ E717), G294 (= G726), P295 (= P727), T297 (≠ L729), D306 (≠ I738), L307 (≠ V739), S308 (≠ P740)
P38503 Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.8 from Methanosarcina thermophila (see 2 papers)
27% identity, 41% coverage: 440:754/760 of query aligns to 5:323/333 of P38503
- C159 (vs. gap) mutation to A: Loss of activity.; mutation to S: No loss of activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P16243 NADP-dependent malic enzyme, chloroplastic; NADP-ME; EC 1.1.1.40 from Zea mays (Maize) (see 3 papers)
27% identity, 47% coverage: 69:425/760 of query aligns to 221:622/636 of P16243
- R237 (≠ A85) mutation to L: Decreases kcat 530-fold. Increases Km for NADP 36-fold and Km for malate 10-fold.
- K255 (= K104) mutation to I: Increases Km for malate 10-fold, and Km for NADP 15-fold. Decreases kcat 200-fold.
- E339 (= E157) mutation to A: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
- A387 (= A208) mutation to G: Decreases kcat 48-fold. Increases Km for NADP 4-fold. Increases Km for malate 6-fold.
- A392 (≠ I213) mutation to G: No effect on kcat. Increases Km for NADP 3.5-fold. Increases Km for malate 2.5-fold.
- KK 435:436 (≠ KS 247:248) mutation to LL: No effect on kcat and on Km for malate. Increases Km for NADP 9-fold.
- Q503 (≠ E306) mutation to E: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
- L544 (≠ I336) mutation to F: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
Sites not aligning to the query:
- 140 F→I: Decreases kcat 8-fold. Decreases Km for NADP 2-fold, increases Km for malate 2-fold.
9e6mA Crystal structure of the g200r mutant from the maize chloroplastic photosynthetic NADP(+)-dependent malic enzyme
28% identity, 39% coverage: 69:367/760 of query aligns to 150:504/565 of 9e6mA
Sites not aligning to the query:
1gq2A Malic enzyme from pigeon liver (see paper)
27% identity, 38% coverage: 79:367/760 of query aligns to 137:480/555 of 1gq2A
- active site: R143 (≠ A85), K161 (= K104), E233 (= E146), D234 (= D147), D256 (= D171), D257 (= D172), N396 (= N297)
- binding manganese (ii) ion: K161 (= K104), E233 (= E146), D234 (= D147), D257 (= D172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R143 (≠ A85), G146 (= G88), N237 (≠ A150), T261 (= T176), G289 (= G204), A290 (= A205), G291 (= G206), E292 (≠ A207), A293 (= A208), V322 (≠ C229), D323 (= D230), S324 (≠ T231), A368 (vs. gap), I370 (≠ V274), L394 (≠ M295), N396 (= N297), G440 (≠ V327), N441 (= N328), N442 (= N329)
- binding oxalate ion: R143 (≠ A85), L145 (= L87), D257 (= D172), N396 (= N297), N442 (= N329)
Sites not aligning to the query:
P40927 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Columba livia (Rock dove) (see 3 papers)
27% identity, 38% coverage: 79:367/760 of query aligns to 138:481/557 of P40927
- D141 (≠ N82) mutation to N: Increases Km for manganese 14-fold. Increases Km for malate 5-fold.
- R144 (≠ A85) binding NADP(+); binding substrate
- K162 (= K104) binding substrate; mutation to A: Decreases kcat 235-fold. no effect on Km for NADP.
- D194 (vs. gap) mutation to N: No effect on Km for manganese. Increases Km for malate 8-fold.
- E234 (= E146) binding Mn(2+)
- D235 (= D147) binding Mn(2+)
- N238 (≠ A150) binding NADP(+)
- D258 (= D172) binding Mn(2+)
- AGEA 291:294 (≠ AGAA 205:208) binding NADP(+)
- S325 (≠ T231) binding NADP(+)
- K340 (≠ W246) mutation to A: Increases Km for NADP 65-fold. No effect on kcat.
- N397 (= N297) binding NADP(+)
- N443 (= N329) binding NADP(+); binding substrate
- D464 (= D350) mutation to N: No effect.
7x11A Crystal structure of me1 in complex with NADPH (see paper)
28% identity, 37% coverage: 79:362/760 of query aligns to 144:482/564 of 7x11A
- binding 6-[(7-methyl-2-propyl-imidazo[4,5-b]pyridin-4-yl)methyl]-2-[2-(1H-1,2,3,4-tetrazol-5-yl)phenyl]-1,3-benzothiazole: N244 (≠ A150), F248 (= F154), I265 (≠ Q173), Q266 (≠ H174), L302 (≠ I210), G303 (≠ A211), H306 (≠ E214), E345 (≠ W246), D470 (= D350)
- binding manganese (ii) ion: E240 (= E146), D241 (= D147), D264 (= D172)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R150 (≠ A85), N244 (≠ A150), T268 (= T176), A297 (= A205), G298 (= G206), E299 (≠ A207), A300 (= A208), D330 (= D230), S331 (≠ T231), K332 (= K232), K346 (= K247), V374 (= V272), A375 (vs. gap), A376 (≠ S273), I377 (≠ V274), L401 (≠ M295), S402 (≠ A296), N403 (= N297), G447 (≠ V327)
6w29A Trypanosoma cruzi malic enzyme in complex with inhibitor (mec013) (see paper)
27% identity, 38% coverage: 77:366/760 of query aligns to 133:483/545 of 6w29A
Sites not aligning to the query:
Query Sequence
>WP_068004602.1 NCBI__GCF_001623255.1:WP_068004602.1
MPTTDKTASSQVSFTDQDALNFHQEGKPGKLEISPTKPMATQRDLSLAYSPGVAVPVLAI
AEDPSRAYDYTTKGNMVAVISNGTAILGLGNLGALASKPVMEGKSVLFKRFADVDSIDLE
VDTANVEEFIDSVKHLGPSFGGINLEDIKAPDCFIIEQRLRELMDIPVFHDDQHGTAIIA
AAGLINAVHMTDRNIGDVKVVCNGAGAAGIACIELVKSMGVPHENVLLCDTKGVIYQGRE
AGMNQWKSAHAVKTDKRTLEDALDGADVFFGVSVKGALTPDMVKTMAPNPIIFAMANPDP
EITPEEAKAVRPDAIIATGRSDYPNQVNNVLGFPYIFRGALDVHATTINDEMKIACANAL
AELAREDVPDEVAAAYRGSRPRFGPEYIIPVPFDPRLISRIPQAVAQAAMDSGVARRPIV
DMEAYGAQLQGRRDPIAGTLQRIISKVRQNPKRIVFAEGEEPAVIRAASAFVSQGLGEAI
LVGREDEILANATSAGIDVSRPGIRLESARSSTRNADYAEYLYSRLQRKGYLQRDCQRLV
NNDRNYWASIMVALGDADGVVTGTTRNYSVALETVSRCIDTKPGHRVIGVSIVITPGKTV
LVADTAVHDMPTSEELADIAEEAAHVARRLGSEPRIAMLAYSTFGHPRGERTERLQEAVK
ILDRRRVDFEYDGEMGADIALNMEKMSAYPFCRLNGPANVLVMPAFHSASIATKLLEELG
GSTLIGPLLVGMDKPVQIVPMGAKDSEIFNMAAIAAYNIT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory