SitesBLAST
Comparing WP_068005521.1 NCBI__GCF_001623255.1:WP_068005521.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
34% identity, 94% coverage: 10:494/515 of query aligns to 4:481/501 of P04983
- K43 (= K49) mutation to R: Loss of transport.
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 43% coverage: 9:232/515 of query aligns to 1:217/241 of 4u00A
3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 45% coverage: 10:240/515 of query aligns to 3:228/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 45% coverage: 10:240/515 of query aligns to 3:228/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
31% identity, 45% coverage: 10:240/515 of query aligns to 3:228/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
31% identity, 45% coverage: 10:240/515 of query aligns to 3:228/242 of 2oljA
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 43% coverage: 10:232/515 of query aligns to 4:231/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 43% coverage: 10:232/515 of query aligns to 4:231/253 of 1g9xB
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
32% identity, 42% coverage: 13:230/515 of query aligns to 6:220/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
32% identity, 42% coverage: 13:230/515 of query aligns to 6:220/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
32% identity, 42% coverage: 13:230/515 of query aligns to 6:220/353 of 1oxuA
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
32% identity, 42% coverage: 13:230/515 of query aligns to 6:220/353 of Q97UY8
- S142 (≠ A153) mutation to A: Decrease in ATPase activity. Can form dimers.
- G144 (= G155) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
- E166 (= E177) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 4:212/369 of P19566
- L86 (= L101) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P178) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ T183) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
30% identity, 43% coverage: 11:229/515 of query aligns to 4:212/371 of P68187
- A85 (≠ T100) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ R121) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ R129) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L132) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E134) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ S139) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G155) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D176) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 3:211/374 of 2awnB
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 1:209/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F20), S35 (≠ N45), G36 (= G46), C37 (≠ A47), G38 (= G48), K39 (= K49), S40 (≠ T50), T41 (= T51), R126 (≠ L147), A130 (≠ K151), S132 (≠ A153), G134 (= G155), Q135 (≠ E156)
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 3:211/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F20), S37 (≠ N45), G38 (= G46), C39 (≠ A47), G40 (= G48), K41 (= K49), S42 (≠ T50), T43 (= T51), Q81 (= Q97), R128 (≠ L147), A132 (≠ K151), S134 (≠ A153), G136 (= G155), Q137 (≠ E156), E158 (= E177), H191 (= H209)
- binding magnesium ion: S42 (≠ T50), Q81 (= Q97)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 3:211/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F20), G38 (= G46), C39 (≠ A47), G40 (= G48), K41 (= K49), S42 (≠ T50), T43 (= T51), R128 (≠ L147), S134 (≠ A153), Q137 (≠ E156)
- binding beryllium trifluoride ion: S37 (≠ N45), G38 (= G46), K41 (= K49), Q81 (= Q97), S134 (≠ A153), G136 (= G155), H191 (= H209)
- binding magnesium ion: S42 (≠ T50), Q81 (= Q97)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
30% identity, 43% coverage: 11:229/515 of query aligns to 3:211/371 of 3puwA