SitesBLAST
Comparing WP_068105332.1 NCBI__GCF_001653335.1:WP_068105332.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P18159 Phosphoglucomutase; PGM; Alpha-phosphoglucomutase; Glucose phosphomutase; EC 5.4.2.2 from Bacillus subtilis (strain 168) (see paper)
37% identity, 89% coverage: 29:523/556 of query aligns to 19:549/581 of P18159
- G162 (= G178) mutation to D: Very low enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
- T240 (≠ V244) mutation to I: Impaired enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
- G407 (= G394) mutation to D: Loss of enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
- D418 (= D406) mutation to N: Impaired enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
Q96G03 Phosphopentomutase; Glucose phosphomutase 2; Phosphodeoxyribomutase; Phosphoglucomutase-2; EC 5.4.2.7; EC 5.4.2.2 from Homo sapiens (Human) (see 2 papers)
36% identity, 87% coverage: 42:526/556 of query aligns to 41:575/612 of Q96G03
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 10 G → D: in dbSNP:rs17856324
O74478 Probable phosphoribomutase; PRM; Phosphoglucomutase 3 homolog; PGM 3 homolog; EC 5.4.2.7 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 91% coverage: 17:521/556 of query aligns to 12:551/587 of O74478
- S149 (= S162) modified: Phosphoserine
Q03262 Phosphoribomutase; PRM; Phosphoglucomutase 3; PGM 3; EC 5.4.2.7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
35% identity, 93% coverage: 5:522/556 of query aligns to 8:586/622 of Q03262
- S158 (= S162) mutation to T: Loss of function.
- P326 (= P308) mutation to G: No effect.
1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
27% identity, 84% coverage: 58:522/556 of query aligns to 5:432/455 of 1wqaA
- active site: R11 (= R64), S101 (= S162), H102 (= H163), K111 (= K172), D243 (= D307), D245 (= D309), D247 (= D311), R248 (= R312), G330 (= G394), R340 (≠ K405)
- binding magnesium ion: S101 (= S162), D243 (= D307), D245 (= D309), D247 (= D311)
O74374 Phosphoglucomutase; PGM; Glucose phosphomutase; EC 5.4.2.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 83% coverage: 59:522/556 of query aligns to 16:511/554 of O74374
- T111 (= T160) modified: Phosphothreonine
- S113 (= S162) modified: Phosphoserine
P36871 Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; EC 5.4.2.2 from Homo sapiens (Human) (see 11 papers)
26% identity, 82% coverage: 59:513/556 of query aligns to 18:507/562 of P36871
- T19 (= T60) to A: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs1320810473
- N38 (≠ Y89) to Y: in CDG1T; strongly reduces solubility; increases aggregation; dbSNP:rs587777402
- Q41 (≠ D92) to R: in CDG1T; reduces solubility; increases aggregation; dbSNP:rs1300651770
- D62 (= D109) to H: in CDG1T; reduces solubility; reduces strongly phosphoglucomutase activity; dbSNP:rs587777403
- K68 (≠ E115) to M: in allele PGM1*7+, allele PGM1*7-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs200390982
- T115 (= T160) to A: in CDG1T; reduces mildly phosphoglucomutase activity; dbSNP:rs121918371
- S117 (= S162) active site, Phosphoserine intermediate; binding via phosphate groupe; modified: Phosphoserine
- G121 (vs. gap) to R: in CDG1T; there is 7% enzyme residual phosphoglucomutase activity; dbSNP:rs398122912
- R221 (≠ V237) to C: in allele PGM1*2+, allele PGM1*2-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs1126728
- D263 (≠ N281) to G: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs1465877146; to Y: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs587777404
- D288 (= D307) binding Mg(2+)
- D290 (= D309) binding Mg(2+)
- G291 (≠ A310) to R: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs772768778
- D292 (= D311) binding Mg(2+)
- G330 (= G346) to R: in CDG1T; decreases mildly solubility; dbSNP:rs777164338
- E377 (= E391) to K: in CDG1T; decreases strongly solubility
- E388 (≠ D404) to K: in CDG1T; decreases strongly solubility; dbSNP:rs1301021797
- Y420 (≠ H439) to H: in allele PGM1*1-, allele PGM1*2-, allele PGM1*3- and allele PGM1*7-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs11208257
- T467 (≠ A476) modified: Phosphothreonine; by PAK1
Sites not aligning to the query:
- 516 L → P: in CDG1T; decreases strongly solubility; dbSNP:rs587777401
2fuvA Phosphoglucomutase from salmonella typhimurium.
27% identity, 84% coverage: 56:522/556 of query aligns to 39:519/545 of 2fuvA
6snqA Crystal structures of human pgm1 isoform 2 (see paper)
25% identity, 77% coverage: 103:531/556 of query aligns to 69:536/566 of 6snqA
- active site: S130 (= S162), H131 (= H163), K143 (= K172), D301 (= D307), D303 (= D309), D305 (= D311), R306 (= R312), G393 (= G394)
- binding 6-O-phosphono-alpha-D-glucopyranose: S130 (= S162), T370 (= T374), G371 (= G375), E389 (= E390), S391 (≠ A392), R512 (= R509), S514 (= S511), R519 (≠ K516)
- binding zinc ion: S130 (= S162), D301 (= D307), D303 (= D309), D305 (= D311)
Sites not aligning to the query:
5jn5A Crystal structure of the d263y missense variant of human pgm1 (see paper)
25% identity, 82% coverage: 59:512/556 of query aligns to 19:507/559 of 5jn5A