SitesBLAST
Comparing WP_068106815.1 NCBI__GCF_001653335.1:WP_068106815.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 98% coverage: 5:283/286 of query aligns to 6:286/286 of P9WNP7
- S122 (= S119) mutation to A: Loss of fatty acyl dehydrogenase activity.
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
48% identity, 98% coverage: 5:283/286 of query aligns to 3:281/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
48% identity, 98% coverage: 5:283/286 of query aligns to 3:281/283 of 4pzdA
- active site: S118 (= S119), H139 (= H140), E151 (= E152), N189 (= N191)
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), T12 (≠ L14), M13 (= M15), D32 (≠ E34), A88 (= A88), A89 (≠ I89), T90 (≠ V90), E91 (= E91), I99 (≠ L99), N116 (= N117), S118 (= S119), N142 (= N143)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
42% identity, 98% coverage: 5:283/286 of query aligns to 28:313/314 of Q16836
- GGGLMG 34:39 (≠ GCGLMG 11:16) binding NAD(+)
- A40 (= A17) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (≠ E34) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (= S50) binding CoA
- K80 (≠ R57) binding CoA
- L86 (vs. gap) to P: in dbSNP:rs4956145
- E122 (= E91) binding NAD(+)
- K127 (= K96) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S119) binding CoA; binding NAD(+)
- Q152 (≠ P122) to H: in dbSNP:rs1051519
- N173 (= N143) binding NAD(+)
- Y226 (≠ F197) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (= P229) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K275) binding NAD(+)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
42% identity, 98% coverage: 5:283/286 of query aligns to 5:290/293 of 1f17A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G13), L14 (= L14), M15 (= M15), D34 (≠ E34), Q35 (≠ S35), A96 (= A88), I97 (= I89), E99 (= E91), K104 (= K96), N124 (= N117), S126 (= S119), H147 (= H140)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
42% identity, 98% coverage: 5:283/286 of query aligns to 5:290/293 of 1f12A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ R57), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), M155 (≠ L148), N197 (= N191), L200 (= L194), P232 (= P226), M233 (≠ Q227), L238 (= L232)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
42% identity, 98% coverage: 5:283/286 of query aligns to 5:290/291 of 1f0yA
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding acetoacetyl-coenzyme a: S50 (= S50), K57 (≠ R57), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), P151 (= P144), P153 (= P146), V154 (= V147), N197 (= N191), P232 (= P226), M233 (≠ Q227), L238 (= L232)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L14 (= L14), M15 (= M15), D34 (≠ E34), Q35 (≠ S35), A96 (= A88), I97 (= I89), E99 (= E91), K104 (= K96), N124 (= N117), S126 (= S119), H147 (= H140), N150 (= N143), V242 (≠ I236), T246 (= T240), K282 (= K275)
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
41% identity, 98% coverage: 5:283/286 of query aligns to 28:313/314 of P00348
- GGGLMG 34:39 (≠ GCGLMG 11:16) binding NAD(+)
- D57 (≠ E34) binding NAD(+)
- E122 (= E91) binding NAD(+)
- K127 (= K96) binding NAD(+)
- S149 (= S119) binding NAD(+)
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
42% identity, 98% coverage: 5:283/286 of query aligns to 1:279/281 of 6aa8E
- active site: S116 (= S119), H137 (= H140), E149 (= E152), N187 (= N191)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ V10), G9 (= G13), T10 (≠ L14), M11 (= M15), R29 (≠ V33), D30 (≠ E34), I31 (≠ S35), A86 (= A88), A87 (≠ I89), E89 (= E91), K94 (= K96), N114 (= N117), S116 (= S119)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
42% identity, 98% coverage: 5:283/286 of query aligns to 2:280/282 of 4kugA
- active site: S117 (= S119), H138 (= H140), E150 (= E152), N188 (= N191)
- binding nicotinamide-adenine-dinucleotide: G10 (= G13), T11 (≠ L14), M12 (= M15), R30 (≠ V33), D31 (≠ E34), A87 (= A88), A88 (≠ I89), E90 (= E91), N115 (= N117), S117 (= S119), H138 (= H140)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
42% identity, 98% coverage: 5:283/286 of query aligns to 2:280/280 of 4kuhA
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
34% identity, 98% coverage: 5:283/286 of query aligns to 362:639/763 of P40939
- E510 (= E152) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa (see paper)
33% identity, 99% coverage: 2:284/286 of query aligns to 299:587/725 of 3zwbA
Sites not aligning to the query:
- active site: 66, 81, 105, 108, 127, 128, 135, 136
- binding (2E)-Hexenoyl-CoA: 25, 26, 28, 64, 65, 66, 67, 68, 101, 103, 105, 108, 136, 161, 280
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
33% identity, 99% coverage: 2:284/286 of query aligns to 299:587/725 of 5omoA
Sites not aligning to the query:
- active site: 66, 71, 81, 105, 108, 127, 128, 135, 136, 254
- binding (s)-3-hydroxydecanoyl-coa: 25, 26, 28, 31, 64, 66, 67, 68, 103, 105, 108, 127, 128, 161, 260, 280
- binding 3-keto-decanoyl-coa: 658
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
33% identity, 99% coverage: 2:284/286 of query aligns to 299:587/725 of 5mgbA
- active site: S415 (= S119), H436 (= H140), E448 (= E152), N486 (= N191)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ V10), G308 (= G11), G310 (= G13), T311 (≠ L14), M312 (= M15), E331 (= E34), S332 (= S35), Q336 (≠ A39), V386 (≠ I89), F387 (≠ V90), E388 (= E91), N413 (= N117), S415 (= S119), H436 (= H140)
Sites not aligning to the query:
- active site: 66, 71, 81, 105, 108, 127, 128, 135, 136, 254
- binding acetoacetyl-coenzyme a: 25, 26, 64, 65, 66, 67, 68, 105, 128, 161
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
33% identity, 99% coverage: 2:284/286 of query aligns to 299:587/725 of 3zwcA