SitesBLAST
Comparing WP_068107728.1 NCBI__GCF_001653335.1:WP_068107728.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6jixA The cyrstal structure of taurine:2-oxoglutarate aminotransferase from bifidobacterium kashiwanohense, in complex with plp and glutamate (see paper)
41% identity, 98% coverage: 7:450/454 of query aligns to 1:443/447 of 6jixA
- active site: W21 (= W27), Y141 (= Y147), D250 (= D253), K279 (= K282)
- binding glutamic acid: S20 (= S26), W21 (= W27), P82 (= P89), A83 (≠ P90), A83 (≠ P90), Y84 (≠ F91), L310 (= L313)
- binding pyridoxal-5'-phosphate: G114 (= G120), A115 (= A121), Y141 (= Y147), H142 (= H148), E217 (= E220), D250 (= D253), V252 (= V255), M253 (= M256), K279 (= K282), L310 (= L313), T311 (= T314)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
38% identity, 93% coverage: 18:438/454 of query aligns to 11:439/448 of 6io1B
- active site: L20 (≠ W27), Y151 (= Y147), D257 (= D253), K286 (= K282)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G119), G118 (= G120), A119 (= A121), N122 (= N124), Y151 (= Y147), H152 (= H148), D257 (= D253), V259 (= V255), I260 (≠ M256), K286 (= K282)
6s54A Transaminase from pseudomonas fluorescens (see paper)
34% identity, 95% coverage: 15:445/454 of query aligns to 8:448/453 of 6s54A
- active site: M20 (≠ W27), Y152 (= Y147), D258 (= D253), K287 (= K282)
- binding pyridoxal-5'-phosphate: G119 (= G120), S120 (≠ A121), Y152 (= Y147), H153 (= H148), G154 (= G149), E225 (= E220), D258 (= D253), V260 (= V255), I261 (≠ M256), K287 (= K282)
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
32% identity, 94% coverage: 19:445/454 of query aligns to 7:435/443 of 6fyqA
- active site: F15 (≠ W27), Y147 (= Y147), D243 (= D253), K272 (= K282)
- binding pyridoxal-5'-phosphate: G114 (= G120), S115 (≠ A121), Y147 (= Y147), H148 (= H148), G149 (= G149), E210 (= E220), D243 (= D253), V245 (= V255), I246 (≠ M256), K272 (= K282)
5g2qA The crystal structure of a s-selective transaminase from arthrobacter sp. With alanine bound (see paper)
34% identity, 83% coverage: 13:388/454 of query aligns to 5:389/461 of 5g2qA
- active site: Y142 (= Y147), D256 (= D253), K285 (= K282)
- binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: G115 (= G120), S116 (≠ A121), Y142 (= Y147), H143 (= H148), V229 (≠ T225), D256 (= D253), V258 (= V255), K285 (= K282), V315 (≠ G312), T317 (= T314)
5g09D The crystal structure of a s-selective transaminase from bacillus megaterium bound with r-alpha-methylbenzylamine (see paper)
35% identity, 84% coverage: 7:388/454 of query aligns to 4:401/473 of 5g09D
- active site: Y147 (= Y147), D268 (= D253), K297 (= K282)
- binding [6-methyl-5-oxidanyl-4-[(~{E})-[(1~{R})-1-phenylethyl]iminomethyl]pyridin-3-yl]methyl dihydrogen phosphate: Y59 (≠ V62), G120 (= G120), S121 (≠ A121), Y147 (= Y147), H148 (= H148), E236 (= E220), A241 (≠ T225), D268 (= D253), V270 (= V255), K297 (= K282)
Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 94% coverage: 27:453/454 of query aligns to 63:496/504 of Q94CE5
- C98 (≠ N63) mutation to Y: In her1-1; loss of E2-hexenal responses.
- G103 (≠ Y68) mutation to N: In her1-2; loss of E2-hexenal responses.
- G271 (≠ N226) mutation to E: In pop2-6; Loss of activity and complete sterility.
- G312 (≠ A267) mutation to D: In pop2-7; loss of activity.
5g0aA The crystal structure of a s-selective transaminase from bacillus megaterium (see paper)
35% identity, 83% coverage: 13:388/454 of query aligns to 5:396/468 of 5g0aA
- active site: F19 (≠ W27), Y142 (= Y147), E231 (= E220), D263 (= D253), L266 (≠ M256), K292 (= K282)
- binding pyridoxal-5'-phosphate: G115 (= G120), S116 (≠ A121), Y142 (= Y147), H143 (= H148), G144 (= G149), E231 (= E220), D263 (= D253), V265 (= V255), L266 (≠ M256), K292 (= K282)
Sites not aligning to the query:
1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
34% identity, 92% coverage: 32:450/454 of query aligns to 20:425/425 of 1szkA
- active site: Y137 (= Y147), E205 (= E220), D238 (= D253), Q241 (≠ M256), K267 (= K282), T296 (= T314), R397 (≠ H422)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G120), S111 (≠ A121), Y137 (= Y147), H138 (= H148), E205 (= E220), D238 (= D253), V240 (= V255), Q241 (≠ M256), K267 (= K282)
Sites not aligning to the query:
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 92% coverage: 32:450/454 of query aligns to 21:426/426 of P22256
- I50 (≠ L61) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GA 120:121) binding pyridoxal 5'-phosphate
- E211 (≠ T225) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (= V255) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (≠ M256) binding pyridoxal 5'-phosphate
- K268 (= K282) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T314) binding pyridoxal 5'-phosphate
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
34% identity, 92% coverage: 32:450/454 of query aligns to 20:425/425 of 1sffA