SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_068112853.1 NCBI__GCF_001653335.1:WP_068112853.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

Q28615 Solute carrier family 13 member 2; Na(+)/dicarboxylate cotransporter 1; NaDC-1; Renal sodium/dicarboxylate cotransporter from Oryctolagus cuniculus (Rabbit) (see paper)
33% identity, 93% coverage: 27:521/535 of query aligns to 9:556/593 of Q28615

query
sites
Q28615

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L83 (≠ A102) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity. Decreases Km value for succinate.
T86 (≠ I105) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
Y228 (≠ W226) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
Y432 (≠ L397) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Decreases Km value for succinate. More sensitive to inhibition by lithium.
T474 (= T439) mutation to C: Does not affect cell membrane localization. Abolishes succinate transport activity.
N525 (= N490) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity.
M539 (= M504) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Insensitive to inhibition by lithium.

Q86YT5 Na(+)/citrate cotransporter; NaCT; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter; Solute carrier family 13 member 5 from Homo sapiens (Human) (see 5 papers)
35% identity, 87% coverage: 56:519/535 of query aligns to 38:540/568 of Q86YT5

query
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Q86YT5

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T142 (= T161) to M: in DEE25; no loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs761917087
G219 (= G231) to E: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150024888; to R: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs144332569
T227 (≠ S239) to M: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs587777577
D243 (= D256) to N: no effect on localization to plasma membrane; no effect on its function in citrate transport; dbSNP:rs142262032
G409 (= G388) mutation to Q: No effect on its function in citrate transport.
I410 (≠ V389) mutation I->A,F: Significant loss of function in citrate transport.; mutation to V: No effect on its function in citrate transport.
L420 (= L399) to P: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150738356
S427 (≠ T406) to L: in DEE25; loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs548065551
L485 (≠ V464) to R: no effect on localization to plasma membrane; reduced function in citrate transport; increased Km and Vmax values compared with that of wild type with citrate as substrate; dbSNP:rs148049520
L488 (≠ V467) to P: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs587777578
D524 (= D503) to H: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs863225448

Sites not aligning to the query:

341:568 natural variant: Missing (in DEE25; loss of localization to plasma membrane; loss of function in citrate transport)
562 modified: carbohydrate, N-linked (GlcNAc...) asparagine

7jsjA Structure of the nact-pf2 complex (see paper)
35% identity, 87% coverage: 56:519/535 of query aligns to 26:446/468 of 7jsjA

query
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7jsjA

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Q

Y

Y

A

M

D

K

P

D

I

T

V

N

F

M

L

L

F

F

L

L

G

G

F

L

L

I

I

V

A

A

I

V

A

A

M

V

Q

E

K

R

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

R

T

T

L

L

R

L

R

W

V

V

G

G

T

A

H

K

P

P

R

A

R

R

I

L

I

M

L

L

G

G

M

F

M

M

I

G

A

V

T

T

A

A

F

L

L

L

S
|
S

M

M

W

W

V
x
I

S
|
S

N
|
N

T
|
T

A

A

T

T

L

A

M

M

L

V

P

P

I

I

A

V

L

E

S

A

V

I

L

L

A

-

L

-

V

-

E

-

N

-

S

-

A

-

G

-

S

-

N

-

Q

-

S

-

T

-

D

-

A

-

E

Q

L

Q

T

R

E

K

R

R

L

L

G

C

G

K

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

A

L

M

L

T

L

L

S

C

V

I

A

C

W

Y

S

A

A

A

S

S

I

I

G

G

L

T

G

A

T

T

L

L

L

T

G
|
G

S
x
T

P

G

P

P

N

N

A

V

I

V

V

L

A

L

G

G

Y

Q

L

M

A

-

S

N

E

E

L

L

D

F

R

P

D

D

-

S

-

K

-

D

-

L

I

V

G

N

F

F

A

A

E

S

W

W

M

F

M

A

L

F

G

A

L

F

P

P

L

N

V

M

V

L

V

V

F

M

L

L

A

L

L

F

A

A

W

W

L

L

L

W

I

L

T

Q

R

F

V

V

I

Y

F

M

R

R

F

S

R

K

L

-

E

K

S

N

I

E

P

K

G

A

A

L

E

K

L

V

I

L

D

Q

T

E

Q

E

I

Y

A

R

E

K

L

L

G

G

A

P

P

L

S

S

R

F

G

A

E

E

K

I

V

N

V

V

L

L

A

I

V

C

F

F

A

F

T

L

A

L

A

V

F

I

F

L

W

W

V

F

V

-

P

-

G

-

V

-

L

-

A

-

S

-

I

-

G

-

D

-

L

-

G

-

D

S

R

R

A

D

P

P

W

E

L

T

G

K

G

Y

F

V

D

S

D

D

T

A

V

T

I

V

A

A

I

I

T

F

A

V

G

A

L

T

V

L

M

L

F

F

L

I

I

V

P

P

G

S

D

Q

E

K

R

P

G

K

R

F

M

Y

T

P

-

P

-

L

L

L

E

D

W

W

H

K

D

V

A

T

E

Q

K

E

G

K

L

V

P

P

W

W

G
|
G

V
x
I

L

V

L

L

F

L

G

G

L

F

S

A

L

L

A

A

S

K

A

G

V

S

A

E

G

A

T

S

G

G

L

L

D

S

A

V

W

W

F

M

G

G

E

K

Q

Q

V

M

G

E

A

P

L

L

G

H

A

A

L

V

P

P

-

P

-

A

I

I

V

T

L

L

L

I

L

L

A

S

A

L

V

L

V

V

A

A

L

V

V

-

L

-

M

-

L

F

T
|
T

E

E

V

C

T
|
T

S
|
S

N
|
N

T

V

A

A

T

T

A

T

T

L

F

F

I

L

P

P

V

I

L

F

G

A

G

S

V

M

A

S

G

R

G

S

L

I

G

G

V

L

D

N

P

P

V

L

T

Y

L

I

L

M

I

L

P

P

A

C

A

T

L

L

A

S

A

A

T

S

C

F

A

A

F

F

M

M

L

L

P

P

V

V

G
x
A

T
|
T

P

P

N

N

A

A

I

I

V

V

F

F

G

T

T

Y

G

G

A

H

V

L

R

K

I

V

A

A

D

D

M

M

A

V

R

K

G

T

G

G

A

V

V

I

L

M

N

N

V

I

G

G

V

V

V

F

L

C

I

V

binding sodium ion: S124 (= S155), I127 (≠ V158), S128 (= S159), N129 (= N160), G172 (= G238), T366 (= T439), T369 (= T442), S370 (= S443), N371 (= N444), A413 (≠ G486), T414 (= T487)
binding (2R)-2-[2-(4-tert-butylphenyl)ethyl]-2-hydroxybutanedioic acid: N129 (= N160), T130 (= T161), T173 (≠ S239), G315 (= G388), I316 (≠ V389), N371 (= N444)

8w6cA Cryoem structure of nadc1 with citrate (see paper)
35% identity, 93% coverage: 27:521/535 of query aligns to 9:505/530 of 8w6cA

query
sites
8w6cA

W

W

G

A

V

Y

R

R

A

S

L

Y

G

L

V

I

V

V

L

F

A

F

A

V

A

P

V

I

Y

L

V

L

A

L

L

P

G

L

G

P

A

I

E

L

L

V

S

P

Q

S

D

K

G

E

R

A

V
x
Y

V

C

A

A

A
x
Y

V

A

G

I

A

I

L

L

M

M

A

A

A

L

W

F

W

W

M

C

T

T

E

E

A

A

L

L

P

P

L

L

A

A

A

V

T

T

A

A

L

L

L

F

P

P

I

L

A
x
I

L

L

L

F

P

P

A
x
M

L

M

T

-

D

-

A

-

L

-

G

G

I

I

A

V

D

D

A

A

T

S

A

E

-

V

-

A

-

V

P

E

Y

Y

A

L

D

K

P

D

I

S

V

N

F

L

L

L

F

F

L

F

G

G

F

L

L

L

I

V

A

A

I

I

A

A

M

V

Q

E

K

H

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

R

T

V

L

L

R

L

R

I

V

V

G

G

T

V

H

R

P

P

R

A

R

P

I

L

I

I

L

L

G

G

M

F

M

M

I

L

A

V

T

T

A

A

F

F

L

L

S
|
S

M

M

W

W

V
x
I

S
|
S

N
|
N

T

T

A

A

T

T

T

S

L

A

M

M

L

V

P

P

I

I

A

A

L

H

S

A

V

V

L

L

A

-

L

-

V

-

E

-

N

D

S

Q

A

L

G

H

S

S

N

S

Q

Q

S

A

T

-

D

-

A

-

E

-

L

-

T

-

E

K

R

H

L

L

G

H

G

L

R

T

E

Q

T

C

M

M

S

S

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

-

L

-

L

L

S

C

V

V

A

C

W

Y

S

S

A

A

S

S

I

I

G

G

L

I

G

A

T

T

L

L

L

T

G
|
G

S

T

P

A

P

P

N

N

A

L

I

V

V

L

A

Q

G

G

Y

Q

L

I

A

N

S

S

E

L

L

F

D

P

R

Q

D

N

-

G

-

N

-

V

I

V

G

N

F

F

A

A

E

S

W

W

M

F

M

S

L

F

G

A

L

F

P

P

L

T

V

M

V

V

V

I

F

L

L

L

A

L

L

L

A

A

W

W

L

L

L

W

I

L

T

Q

R

I

V

L

I

F

-

L

-

G

F

F

R

N

F

F

R

R

-

K

-

N

-

F

-

G

-

I

-

G

-

E

-

K

-

M

L

Q

E

E

S

Q

I

Q

P

Q

G

A

A

Y

E

C

L

V

I

I

D

Q

T

T

Q

E

I

H

A

R

E

L

L

L

G

G

A

P

P

M

S

T

R

F

G

A

E

E

K

K

V

A

V

I

L

S

A

I

V

L

F

F

A

V

T

I

A

L

A

V

F

L

W

W

V

F

V

T

-

R

-

E

P

P

G

G

V

F

L

F

A

L

S

G

I

W

G

G

D

N

L

L

G

A

-

F

D

P

R

N

A

A

P

K

W

G

L

E

G

S

G

M

F

V

D

S

D

D

T

G

V

T

I

V

A

A

I

I

T

F

A

I

G

G

L

I

V

I

M

M

F

F

L

I

I

I

P

P

G

S

-

K

-

F

-

P

-

G

-

L

-

T

-

Q

-

D

-

P

D

E

E

N

R

P

G

G

R

K

M

L

T

K

-

A

-

P

-

L

-

G

-

L

L

L

E

D

W

W

H

K

D

T

A

V

E

N

K

Q

G

K

L

M

P

P

W

W

G

N

V

I

L

V

L

L

F

L

G

G

L

Y

S

A

L

L

A

A

S

K

A

G

V

S

A

E

G

R

T

S

G

G

L

L

D

S

A

E

W

W

F

L

G

G

E

N

Q

K

V

L

G

T

A

P

L

L

G

Q

A

S

L

V

P

P

I

A

V

P

L

A

L

I

L

A

A

I

V

L

V

S

A

L

L

L

V

V

L

A

M

T

L

F

T
|
T

E

E

V

C

T
|
T

S
|
S

N
|
N

T

V

A

A

T

T

A

T

T

I

F

F

I

L

P

P

V

I

L

L

G

A

G

S

V

M

A

A

G

Q

G

A

L

I

G

C

V

L

D

H

P

P

V

L

T

Y

L

V

L

M

I

L

P

P

A

C

A

T

L

L

A

A

A

T

T

S

C

L

A

A

F

F

M

M

L

L

P

P

V

V

G
x
A

T

T

P

P

N

N

A

A

I

I

V

V

F

F

G

S

T

F

G

G

A

D

V

L

R

K

I

V

A

L

D

D

M

M

A

A

R

R

G

A

G

G

A

F

V

L

L

L

N

N

V

I

G

G

V

V

V

L

L

V

I

I

T

A

M

L

binding sodium ion: S136 (= S155), I139 (≠ V158), S140 (= S159), N141 (= N160), G191 (= G238), T423 (= T439), T426 (= T442), S427 (= S443), N428 (= N444), A470 (≠ G486)
binding cholesterol hemisuccinate: Y37 (≠ V55), Y40 (≠ A58), I65 (≠ A83), M69 (≠ A87)

8w6gA Nadc1 with inhibitor aca (see paper)
34% identity, 93% coverage: 27:521/535 of query aligns to 9:502/527 of 8w6gA

query
sites
8w6gA

W

W

G

A

V

Y

R

R

A

S

L

Y

G

L

V

I

V

V

L

F

A

F

A

V

A

P

V

I

Y

L

V

L

A

L

L

P

G

L

G

P

A

I

E

L

L

V

S

P

Q

S

D

K

G

E

R

A

V
x
Y

V

C

A

A

A
x
Y

V

A

G

I

A

I

L

L

M

M

A

A

A

L

W

F

W

W

M

C

T

T

E

E

A

A

L

L

P

P

L

L

A

A

A

V

T

T

A

A

L

L

L

F

P

P

I

L

A

I

L
|
L

L

F

P

P

A
x
M

L

M

T

-

D

-

A

-

L

-

G

G

I

I

A

V

D

D

A

A

T

S

A

E

-

V

-

A

-

V

P

E

Y

Y

A

L

D

K

P

D

I

S

V

N

F

L

L

L

F

F

L

F

G

G

F

L

L

L

I

V

A

A

I

I

A

A

M

V

Q

E

K

H

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

R

T

V

L

L

R

L

R

I

V

V

G

G

T

V

H

R

P

P

R

A

R

P

I

L

I

I

L

L

G

G

M

F

M

M

I

L

A

V

T

T

A

A

F

F

L

L

S

S

M

M

W

W

V

I

S

S

N

N

T

T

A

A

T

T

T

S

L

A

M

M

L

V

P

P

I

I

A

A

L

H

S

A

V

V

L

L

A

-

L

-

V

-

E

-

N

-

S

-

A

-

G

-

S

D

N

Q

Q

L

S

H

T

S

D

S

A

Q

A

A

E

H

L

L

T

T

E

Q

R

C

L

M

G

S

G

-

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

-

L

-

L

L

S

C

V

V

A

C

W

Y

S

S

A

A

S

S

I

I

G

G

L

I

G

A

T

T

L

L

L

T

G

G

S

T

P

A

P

P

N

N

A

L

I

V

V

L

A

Q

G

G

Y

Q

L

I

A

N

S

S

E

L

L

F

D

P

R

Q

D

N

-

G

-

N

-

V

I

V

G

N

F

F

A

A

E

S

W

W

M

F

M

S

L

F

G

A

L

F

P

P

L

T

V

M

V

V

V

I

F

L

L

L

A

L

L

L

A

A

W

W

L

L

L
x
W

I

L

T

Q

R

I

V
x
L

I

F

-

L

-

G

F

F

R

N

F

F

R

R

-

K

-

N

-

F

-

G

-

I

-

G

-

E

-

K

-

M

L

Q

E

E

S

Q

I

Q

P

Q

G

A

A

Y

E

C

L

V

I

I

D

Q

T

T

Q

E

I

H

A

R

E

L

L

L

G

G

A

P

P

M

S

T

R

F

G

A

E

E

K

K

V

A

V

I

L

S

A

I

V

L

F

F

A

V

T

I

A

L

A

V

F

L

W

W

V

F

V

T

-

R

-

E

P

P

G

G

V

F

L

F

A

L

S

G

I

W

G

G

D

N

L

L

G

A

-

F

D

P

R

N

A

A

P

K

W

G

L

E

G

S

G

M

F

V

D

S

D

D

T

G

V

T

I

V

A

A

I

I

T

F

A

I

G

G

L

I

V

I

M

M

F

F

L

I

I

I

P

P

G

S

-

K

-

F

-

P

-

G

-

L

-

T

-

Q

-

D

-

P

D

E

E

N

R

P

G

G

R

K

M

L

T

K

-

A

-

P

-

L

-

G

-

L

L

L

E

D

W

W

H

K

D

T

A

V

E

N

K

Q

G

K

L

M

P

P

W

W

G

N

V

I

L

V

L

L

F

L

G

G

L

Y

S

A

L

L

A

A

S

K

A

G

V

S

A

E

G

R

T

S

G

G

L

L

D

S

A

E

W

W

F

L

G

G

E

N

Q

K

V

L

G

T

A

P

L

L

G

Q

A

S

L

V

P

P

I

A

V

P

L

A

L

I

L

A

A

I

V

L

V

S

A

L

L

L

V

V

L

A

M

T

L

F

T

T

E

E

V

C

T

T

S

S

N

N

T

V

A

A

T

T

A

T

T

I

F

F

I

L

P

P

V

I

L

L

G

A

G

S

V

M

A

A

G

Q

G

A

L

I

G

C

V

L

D

H

P

P

V

L

T

Y

L

V

L

M

I

L

P

P

A

C

A

T

L

L

A

A

A

T

T

S

C

L

A

A

F

F

M

M

L

L

P

P

V

V

G

A

T

T

P

P

N

N

A

A

I

I

V

V

F

F

G

S

T

F

G

G

A

D

V

L

R

K

I

V

A

L

D

D

M

M

A
|
A

R
|
R

G

A

G

G

A
x
F

V

L

L

L

N

N

V

I

G

G

V

V

V

L

L

V

I

I

T

A

M

L

binding 2-[[(~{E})-3-(4-pentylphenyl)prop-2-enoyl]amino]benzoic acid: W233 (≠ L280), L237 (≠ V284), A486 (= A505), R487 (= R506), F490 (≠ A509)
binding cholesterol hemisuccinate: Y37 (≠ V55), Y40 (≠ A58), L66 (= L84), M69 (≠ A87)

8w6nB Nas1 with sulfate in in/out state (see paper)
34% identity, 86% coverage: 62:521/535 of query aligns to 36:460/481 of 8w6nB

query
sites
8w6nB

L

V

M

V

A

A

A

T

W

F

W

W

M

L

T

T

E

E

A

A

L

L

P

P

L

L

A

S

A

V

T

T

A

A

L

L

P

P

I

S

A

L

L

M

L

L

P

P

A

M

L

F

T

G

D

I

A

M

L

P

G

S

I

K

A

K

D

V

A

A

T

S

A

A

P

Y

Y

F

A

K

D

D

P

-

I

F

V

H

F

L

L

L

F

L

L

I

G

G

G

V

F

I

L

C

I

L

A

A

I

T

A

S

M

I

Q

E

K

K

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

K

T

M

L

V

R

M

V

V

G

G

T

V

H

N

P

P

R

A

R

W

I

L

I

T

L

L

G

G

M

F

M

M

I

S

A

S

T

T

A

A

F

F

L

L

S

S

M

M

W

W

V

L

S

S

N
|
N

T
|
T

A

S

T

T

T

A

L

A

M

M

M

V

L

M

P

P

I

I

A

A

L

E

S

A

V

V

L

V

A

-

L

-

V

-

E

-

N

-

S

-

A

-

G

-

S

-

N

Q

Q

Q

S

I

T

I

D

N

A

A

A

G

E

H

L

V

T

T

E

R

R

K

L

L

G

T

G

-

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

-

L

-

L

C

L

L

S

C

V

I

A

A

W

Y

S

S

A

S

S

T

I

I

G

G

L

L

G

T

T

T

L

I

L

T

G

G

S
x
T

P
x
S

P

T

N

N

A

L

I

I

V

F

A

A

G

E

Y

Y

L

F

A

N

S

T

E

R

L

Y

D

P

-

D

-

C

R

R

D

C

I

L

G

N

F

F

A

G

E

S

W

W

M

F

M

T

L

F

G

S

L

F

P

P

L

A

V

A

V

L

V

I

F

I

L

L

A

L

L

L

A

S

W

W

L

I

L

W

I

L

T

Q

R

W

V

L

I

F

F

-

R

-

F

-

R

-

L

L

E

G

S

Q

I

Q

P

K

G

A

G

C

A

A

E

E

L

V

I

I

D

K

T

Q

Q

E

I

Y

A

Q

E

K

L

L

G

G

A

P

P

I

S

R

R

Y

G

Q

E

E

K

I

V

V

V

T

L

L

A

V

V

L

F

F

A

I

T

I

A

M

A

A

F

L

W

W

V

F

V

S

-

R

-

D

P

P

G

G

V

F

L

V

A

P

S

G

I

W

G

S

D

A

L

L

G

F

D

S

R

E

A

Y

P

P

W

-

L

-

G

G

G

F

F

A

D

T

D

D

T

S

V

T

I

V

A

A

I

L

T

L

A

I

G

G

L

L

V

L

M

F

F

F

L

L

I

I

P

P

G

A

D

K

E

T

R

Y

G

S

R

P

M

L

T

-

L

I

E

T

W

W

H

K

D

E

A

F

E

Q

K

S

G

F

L

M

P

P

W

W

G

D

V

I

L

A

L

I

L

L

F

V

G

G

L

F

S

A

L

L

A

A

S

D

A

G

V

C

A

E

G

E

T

S

G

G

L

L

D

S

A

K

W

W

F

I

G

G

E

N

Q

K

V

L

G

S

A

P

L

L

G

G

A

S

L

L

P

P

I

A

V

W

L

L

L

I

A

L

A

I

V

S

A

L

L

M

V

V

L

T

M

S

L

L

T

T

E

E

V

V

T

A

S

S

N

N

T

P

A

A

T

T

A

I

A

T

T

L

F

F

I

L

P

P

V

I

L

L

G

S

G

P

V

L

A

A

G

E

G

A

L

I

G

H

V

V

D

N

P

P

V

L

T

Y

L

I

L

L

I

I

P

P

A

S

A

T

L

L

A

C

A

T

T

S

C

F

A

A

F

F

M

L

L

L

P

P

V

V

G

A

T

N

P

P

N

N

A

A

I

I

V

V

F

F

G

S

T

Y

G

G

A

H

V

L

R

K

I

V

A

I

D

D

M

M

A

V

R

K

G

A

G

G

A

L

V

G

L

V

N

N

V

I

V

V

G

G

V

V

V

A

L

V

I

V

T

M

M

L

binding sulfate ion: N133 (= N160), T134 (= T161), T181 (≠ S239), S182 (≠ P240)

8w6nA Nas1 with sulfate in in/out state (see paper)
34% identity, 86% coverage: 62:521/535 of query aligns to 40:472/494 of 8w6nA

query
sites
8w6nA

L

V

M

V

A

A

A

T

W

F

W

W

M

L

T

T

E

E

A

A

L

L

P

P

L

L

A

S

A

V

T

T

A

A

L

L

P

P

I

S

A

L

L

M

L

L

P

P

A

M

L

F

T

G

D

I

A

M

L

P

G

S

I

K

A

K

D

V

A

A

T

S

A

A

P

Y

Y

F

A

K

D

D

P

-

I

F

V

H

F

L

L

L

F

L

L

I

G

G

G

V

F

I

L

C

I

L

A

A

I

T

A

S

M

I

Q

E

K

K

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

K

T

M

L

V

R

M

V

V

G

G

T

V

H

N

P

P

R

A

R

W

I

L

I

T

L

L

G

G

M

F

M

M

I

S

A

S

T

T

A

A

F

F

L

L

S
|
S

M

M

W

W

V

L

S

S

N
|
N

T

T

A

S

T

T

T

A

L

A

M

M

M

V

L

M

P

P

I

I

A

A

L

E

S

A

V

V

L

V

A

Q

L

Q

V

I

E

N

N

A

S

E

A

A

G

K

S

G

N

H

Q

-

S

-

T

-

D

-

A

-

E

-

L

V

T

T

E

R

R

K

L

L

G

T

G

-

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

-

L

-

L

C

L

L

S

C

V

I

A

A

W

Y

S

S

A

S

S

T

I

I

G

G

G
|
G

L

L

G

T

T

T

L

I

L

T

G
|
G

S
x
T

P

S

P

T

N

N

A

L

I

I

V

F

A

A

G

E

Y

Y

L

F

A

N

S

T

E

R

L

Y

D

P

-

D

-

C

R

R

D

C

I

L

G

N

F

F

A

G

E

S

W

W

M

F

M

T

L

F

G

S

L

F

P

P

L

A

V

A

V

L

V

I

F

I

L

L

A

L

L

L

A

S

W

W

L

I

L

W

I

L

T

-

R

Q

V

W

I

L

F

F

R

L

F

G

R

T

L

V

E

Q

S

Q

I

-

P

K

G

A

G

C

A

A

E

E

L

V

I

I

D

K

T

Q

Q

E

I

Y

A

Q

E

K

L

L

G

G

A

P

P

I

S

R

R

Y

G

Q

E

E

K

I

V

V

V

T

L

L

A

V

V

L

F

F

A

I

T

I

A

M

A

A

F

L

W

W

V

F

V

S

-

R

-

D

P

P

G

G

V

F

L

V

A

P

S

G

I

W

G

S

D

A

L

L

G

F

D

S

R

E

A

Y

P

P

W

-

L

-

G

G

G

F

F

A

D

T

D

D

T

S

V

T

I

V

A

A

I

L

T

L

A

I

G

G

L

L

V

L

M

F

F

F

L

L

I

I

P

P

G

A

D

K

E

T

R

L

G

F

R

D

M

Y

T

S

-

P

-

L

L

I

E

T

W

W

H

K

D

E

A

F

E

Q

K

S

G

F

L

M

P

P

W

W

G

D

V

I

L

A

L

I

L

L

F

V

G

G

L

F

S

A

L

L

A

A

S

D

A

G

V

C

A

E

G

E

T

S

G

G

L

L

D

S

A

K

W

W

F

I

G

G

E

N

Q

K

V

L

G

S

A

P

L

L

G

G

A

S

L

L

P

P

I

A

V

W

L

L

L

I

A

L

A

I

V

S

A

L

L

M

V

V

L

T

M

S

L

L

T

T

E

E

V

V

T

A

S

S

N

N

T

P

A

A

T

T

A

I

A

T

T

L

F

F

I

L

P

P

V

I

L

L

G

S

G

P

V

L

A

A

G

E

G

A

L

I

G

H

V

V

D

N

P

P

V

L

T

Y

L

I

L

L

I

I

P

P

A

S

A

T

L

L

A

C

A

T

T

S

C

F

A

A

F

F

M

L

L

L

P

P

V

V

G

A

T

N

P

P

N

N

A

A

I

I

V

V

F

F

G

S

T

Y

G

G

A

H

V

L

R

K

I

V

A

I

D

D

M

M

A

V

R

K

G

A

G

G

A

L

V

G

L

V

N

N

V

I

V

V

G

G

V

V

V

A

L

V

I

V

T

M

M

L

binding sodium ion: S132 (= S155), N137 (= N160), G181 (= G232), G187 (= G238), T188 (≠ S239)

8w6hA Nas1 with sulfate - in/in state (see paper)
33% identity, 86% coverage: 62:521/535 of query aligns to 35:441/462 of 8w6hA

query
sites
8w6hA

L

V

M

V

A

A

A

T

W

F

W

W

M

L

T

T

E

E

A

A

L

L

P

P

L

L

A

S

A

V

T

T

A

A

L

L

P

P

I

S

A

L

L

M

L

L

P

P

A

M

L

F

T

G

D

I

A

M

L

P

G

S

I

K

A

K

D

V

A

A

T

S

A

A

P

Y

Y

F

A

K

D

D

P

-

I

F

V

H

F

L

L

L

F

L

L

I

G

G

G

V

F

I

L

C

I

L

A

A

I

T

A

S

M

I

Q

E

K

K

W

W

N

N

L

L

H

H

R

K

R

R

I

I

A

A

L

L

L

K

T

M

L

V

R

M

V

V

G

G

T

V

H

N

P

P

R

A

R

W

I

L

I

T

L

L

G

G

M

F

M

M

I

S

A

S

T

T

A

A

F

F

L

L

S
|
S

M

M

W

W

V
x
L

S
|
S

N
|
N

T
|
T

A

S

T

T

T

A

L

A

M

M

M

V

L

M

P

P

I

I

A

A

L

E

S

A

V

V

L

V

A

-

L

-

V

-

E

-

N

-

S

-

A

-

G

-

S

-

N

Q

Q

Q

S

I

T

I

D

N

A

A

A

G

E

H

L

V

T

T

E

R

R

K

L

L

G

T

G

C

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

-

A

-

L

-

L

L

S

C

V

I

A

A

W

Y

S

S

A

S

S

T

I

I

G

G

L

L

G

T

T

T

L

I

L

T

G
|
G

S
x
T

P
x
S

P

T

N

N

A

L

I

I

V

F

A

A

G

E

Y

Y

L

F

A

N

S

T

E

R

L

Y

D

P

-

D

-

C

R

R

D

C

I

L

G

N

F

F

A

G

E

S

W

W

M

F

M

T

L

F

G

S

L

F

P

P

L

A

V

A

V

L

V

I

F

I

L

L

A

L

L

L

A

S

W

W

L

I

L

W

I

L

T

Q

R

W

V

L

I

F

F

L

R

G

F

-

R

-

L

-

E

-

S

-

I

-

P

-

G

-

A

-

E

-

L

-

I

I

D

K

T

Q

Q

E

I

Y

A

Q

E

K

L

L

G

G

A

P

P

I

S

R

R

Y

G

Q

E

E

K

I

V

V

V

T

L

L

A

V

V

L

F

F

A

I

T

I

A

M

A

A

F

L

W

W

V

F

V

-

P

-

G

-

V

-

L

-

A

-

S

-

I

-

G

-

D

-

L

-

G

-

D

S

R

R

A

P

P

-

W

-

L

-

G

G

G

F

F

A

D

T

D

D

T

S

V

T

I

V

A

A

I

L

T

L

A

I

G

G

L

L

V

L

M

F

F

F

L

L

I

I

P

P

G

A

D

K

E

T

R

L

G

T

R

A

M

F

T

D

-

Y

-

S

-

P

-

L

L

I

E

T

W

W

H

K

D

E

A

F

E

Q

K

S

G

F

L

M

P

P

W

W

G

D

V

I

L

A

L

I

L

L

F

V

G

G

L

F

S

A

L

L

A

A

S

D

A

G

V

C

A

E

G

E

T

S

G

G

L

L

D

S

A

K

W

W

F
x
I

G

G

E

N

Q

K

V

L

G

S

A

P

L

L

G

G

A

S

L

L

P

P

I

A

V

W

L

L

L

I

A

L

A

I

V

S

A

L

L

M

V

V

L

T

M

S

L

L

T

T

E

E

V

V

T

A

S

S

N

N

T

P

A

A

T

T

A

I

A

T

T

L

F

F

I

L

P
|
P

V

I

L

L

G

S

G

P

V

L

A

A

G

E

G

A

L

I

G

H

V

V

D

N

P

P

V

L

T

Y

L

I

L

L

I

I

P

P

A

S

A

T

L

L

A

C

A

T

T

S

C

F

A

A

F

F

M

L

L

L

P

P

V

V

G

A

T

N

P

P

N

N

A

A

I

I

V

V

F

F

G

S

T

Y

G

G

A

H

V

L

R

K

I

V

A

I

D

D

M

M

A

V

R

K

G

A

G

G

A

L

V

G

L

V

N

N

V

I

V

V

G

G

V

V

V

A

L

V

I

V

T

M

M

L

binding cholesterol: I332 (≠ F412), P373 (= P453)
binding sodium ion: S127 (= S155), L130 (≠ V158), S131 (= S159), N132 (= N160), G179 (= G238), T180 (≠ S239)
binding sulfate ion: T133 (= T161), S181 (≠ P240)

7t9gA Structure of vcindy-na+ (see paper)
36% identity, 86% coverage: 61:520/535 of query aligns to 36:437/445 of 7t9gA

query
sites
7t9gA

A

A

L

F

M

I

A

A

A

V

W

L

W

W

M

L

T

T

E

E

A

A

L

L

P

H

L

V

A

T

A

V

T

T

A

A

L

I

L

L

P

-

I

-

A

-

L

-

L

V

P

P

A

V

L

M

T

A

D

V

A

F

L

F

G

G

I

I

A

F

D

E

A

T

T

Q

A

A

P

A

-

L

-

N

Y

F

A

A

D

N

P

S

I

I

V

I

F

F

L

L

F

F

L

L

G

G

F

F

L

A

I

L

A

A

I

A

A

A

M

M

Q

H

K

H

W

Q

N

G

L

L

H

D

R

K

R

V

I

I

A

A

L

D

L

K

T

V

L

L

R

A

R

M

V

A

G

Q

T

G

H

K

P

M

R

S

R

V

I

A

I

V

L

F

G

M

M

L

M

F

I

G

A

V

T

T

A

A

F

L

L

L

S
|
S

M

M

W

W

V

I

S
|
S

N
|
N

T

T

A

A

T

T

T

A

L

A

M

M

L

L

P

P

I

L

A

V

L

L

S

G

V

V

L

L

A

S

L

K

V

V

V

-

E

-

N

-

S

D

A

A

G

D

S

K

N

Q

Q

R

S

S

T

T

D

Y

A

-

E

-

L

-

T

-

E

-

R

-

L

-

G

-

R

-

E

-

T

-

M

-

S

-

D

-

V

-

I

-

D

-

V

-

D

-

V

-

R

-

L

-

F

-

G

-

V

V

A

F

L

V

L

L

S

G

V

V

A

A

W

Y

S

S

A

A

S

S

I

I

G

G

L

I

G

A

T

T

L

L

L

V

G
|
G

S

S

P

P

N

N

A

A

I

I

V

A

A

A

G

A

Y

-

L

-

A

-

S

-

E

-

L

-

D

E

R

V

D

G

I

L

G

S

F

F

A

T

E

D

W

W

M

M

M

K

L

F

G

G

L

L

P

P

L

T

V

A

V

M

F

M

L

L

A

P

L

M

A

A

W

-

L

-

I

-

T

-

R

I

V

A

I

I

F

L

R

Y

F

F

R

L

L

L

E

K

S

P

I

T

P

L

G

N

G

G

A

M

E

F

L

E

I

L

D

D

T

R

Q

-

I

-

A

-

E

-

L

-

G

-

A

A

P

P

-

V

-

N

-

W

S

D

R

K

G

G

E

K

K

V

V

V

V

T

L

L

A

G

V

I

F

F

A

G

T

L

A

T

A

V

F

F

F

L

W

W

V

I

V

F

P

S

G

S

V

P

L

I

A

N

S

A

I

A

G

-

D

-

L

-

G

-

D

-

R

-

A

-

P

-

W

-

L

L

G

G

F

F

D

K

-

S

-

F

D

D

T

T

V

L

I

V

A

A

I

L

T

G

A

A

G

I

L

L

V

M

M

L

F

-

L

-

I

-

P

-

G

-

D

-

E

S

R

F

G

A

R

R

M

V

T

-

L

V

E

H

W

W

H

K

D

E

A

I

E

Q

K

K

G

T

L

A

P

D

W

W

G

G

V

V

L

L

F

F

G

G

L

L

S

C

L

L

A

S

S

N

A

V

V

L

A

K

G

Q

T

T

G

G

L

T

D

S

A

V

W

F

F

L

-

A

-

N

-

A

-

L

G

S

E

D

Q

M

V

V

G

S

A

H

L

M

G

G

A

I

L

F

P

V

I

V

V

I

L

L

L

V

A

A

A

T

V

-

A

-

L

F

V

V

L

V

M

F

L

L

T
|
T

E

E

V

F

T
x
A

S
|
S

N
|
N

T

T

A

A

T

S

A

A

T

L

F

L

I

I

P

P

V

V

L

F

G

A

G

T

V

V

A

A

G

E

G

A

L

F

G

G

V

M

D

S

P

P

V

V

T

L

L

L

L

S

I

V

P

L

A

I

A

A

L

V

A

A

T

S

C

C

A

A

F

F

M

M

L

L

P

P

V

V

G
x
A

T

T

P

P

N

N

A

A

I

I

V

V

F

F

G

A

T

S

G

G

A

H

V

I

R

K

I

Q

A

S

D

E

M

M

A

M

R

R

G

V

G

G

A

L

V

Y

L

L

N

N

V

I

V

A

G

C

V

I

V

G

L

L

I

L

T

T

binding sodium ion: S129 (= S155), S133 (= S159), N134 (= N160), G182 (= G238),