SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_068115344.1 NCBI__GCF_001653335.1:WP_068115344.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
51% identity, 88% coverage: 17:151/153 of query aligns to 2:140/141 of 6wf6A

query
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active site: H7 (= H22), E43 (= E58), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding cobalt (ii) ion: H7 (= H22), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
51% identity, 88% coverage: 17:151/153 of query aligns to 2:140/144 of 6xbqA

query
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6xbqA

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active site: H7 (= H22), E43 (= E58), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding carboxymethyldethia coenzyme *a: Q39 (= Q54), Q60 (= Q71), A70 (≠ T81), K73 (= K84), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), F122 (= F133), P125 (= P136), K126 (= K137), L132 (= L143)
binding cobalt (ii) ion: H7 (= H22), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
51% identity, 88% coverage: 17:151/153 of query aligns to 2:140/144 of 6wfiA

query
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6wfiA

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active site: H7 (= H22), E43 (= E58), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding cobalt (ii) ion: H7 (= H22), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H22), Q39 (= Q54), Q60 (= Q71), A70 (≠ T81), K73 (= K84), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), G114 (= G125), S115 (≠ T126), I120 (= I131), F122 (= F133), P125 (= P136), L132 (= L143), E134 (= E145)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
51% identity, 88% coverage: 17:151/153 of query aligns to 2:140/144 of 6wf7A

query
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6wf7A

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active site: H7 (= H22), E43 (= E58), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (= Q54), E43 (= E58), Q60 (= Q71), A70 (≠ T81), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), G114 (= G125), S115 (≠ T126), F122 (= F133), P125 (= P136), K126 (= K137), L132 (= L143), E134 (= E145)
binding methylmalonyl-coenzyme a: Q39 (= Q54), Q60 (= Q71), A70 (≠ T81), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), G114 (= G125), S115 (≠ T126), I120 (= I131), F122 (= F133), P125 (= P136), K126 (= K137), L132 (= L143), E134 (= E145)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
51% identity, 88% coverage: 17:150/153 of query aligns to 2:139/139 of 6wfhA

query
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active site: H7 (= H22), E43 (= E58), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding cobalt (ii) ion: H7 (= H22), Q60 (= Q71), H84 (≠ Q95), E134 (= E145)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H22), Q39 (= Q54), Q60 (= Q71), A70 (≠ T81), K73 (= K84), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), G114 (= G125), S115 (≠ T126), F122 (= F133), P125 (= P136), K126 (= K137), L132 (= L143), E134 (= E145)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
50% identity, 88% coverage: 17:151/153 of query aligns to 2:140/140 of 6xbtA

query
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6xbtA

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active site: H7 (= H22), E43 (= E58), A60 (≠ Q71), H84 (≠ Q95), E134 (= E145)
binding cobalt (ii) ion: H7 (= H22), H84 (≠ Q95), E134 (= E145)
binding coenzyme a: Q39 (= Q54), A70 (≠ T81), K73 (= K84), W74 (≠ F85), K77 (≠ R88), H83 (≠ Q94), L107 (= L118), F122 (= F133), P125 (= P136), K126 (= K137), L132 (= L143)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H22), Q39 (= Q54), A70 (≠ T81), K73 (= K84), W74 (≠ F85), H83 (≠ Q94), H84 (≠ Q95), L107 (= L118), G114 (= G125), S115 (≠ T126), P125 (= P136), K126 (= K137), L132 (= L143), E134 (= E145)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
37% identity, 86% coverage: 18:148/153 of query aligns to 3:131/138 of 3oa4A

query
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V

L

L

V

Y

E
|
E

I

F

V

C

E

E

active site: H7 (= H22), I43 (≠ E58), E54 (≠ Q71), H78 (≠ Q95), E128 (= E145)
binding zinc ion: H7 (= H22), E54 (≠ Q71), H78 (≠ Q95), E128 (= E145)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
35% identity, 83% coverage: 20:146/153 of query aligns to 48:173/176 of Q96PE7

query
sites
Q96PE7

I

L

D

N

H
|
H

V

V

G

A

M

I

A

A

V

V

P

P

D

D

L

L

D

E

V

K

A

A

I

A

A

A

F

F

Y

Y

R

K

D

N

T

I

F

L

G

G

M

A

T

Q

L

V

A

S

H

E

E

A

E

V

V

P

N

L

E

P

E

E

Q

H

G

G

V

V

R

S

E

V

A

V

M

F

M

V

A

N

V

L

G

G

D

N

S

T

T

K

S

-

C

-

V

M

Q

E

L

L

L

L

A

H

P

P

L

L

S

G

P
x
R

E

D

S

S

T

P

I

I

A

A

K

G

F

F

I

L

D

Q

R

K

N

N

G

K

P

A

G

G

-

G

C

M

Q

H

Q
x
H

I

I

A

C

Y

I

R

E

V

V

T

D

D

N

V

I

D

N

A

A

V

A

S

V

A

M

T

D

L

L

R

K

E

K

R

K

G

K

L

I

R

R

M

S

L

L

Y

S

D

E

E

E

P

V

R

K

R

I

G

G

T

A

S

H

D

G

S

K

R

P

I

V

N

I

F

F

V

L

H

H

P

P

K

K

D

D

A

C

G

G

G

G

V

V

L

L

V

V

E
|
E

I

L

H50 (= H22) binding Co(2+)
R104 (≠ P78) to L: in dbSNP:rs6748672
H122 (≠ Q95) binding Co(2+)
E172 (= E145) binding Co(2+)

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
34% identity, 83% coverage: 20:146/153 of query aligns to 12:135/138 of 6qh4C

query
sites
6qh4C

I

L

D

N

H
|
H

V

V

G

A

M

I

A

A

V

V

P

P

D

D

L

L

D

E

V

K

A

A

I

A

A

A

F

F

Y

Y

R

K

D

N

T

I

F

L

G

G

M

A

T

Q

L

V

A

S

H

E

E

A

E

V

V

P

N

L

E

P

E

E

Q

H

G

G

V

V

R

S

E
x
V

A

V

M

F

M

V

A

N

V

L

G

G

D

N

S

T

T

K

S

-

C

-

V

M

Q
x
E

L

L

L

L

A

H

P

P

L

L

S

G

P

L

E

D

S

S

T

P

I

I

A

A

K

G

F

F

I

L

D

Q

R

K

N

N

G

K

P

A

-

G

G

G

C

M

Q

H

Q
x
H

I

I

A

C

Y

I

R

E

V

V

T

D

D

N

V

I

D

N

A

A

V

A

S

V

A

M

T

D

L

L

R

K

E

K

R

K

G

K

L

I

R

C

M

S

L

L

Y

-

D

-

E

S

P

V

R

K

R

I

G

G

T

A

S

H

D

G

S

K

R

P

I

V

N

I

F

F

V

L

H

H

P

P

K

K

D

D

A

C

G

G

G

G

V

V

L

L

V

V

E
|
E

I

L

active site: H14 (= H22), V50 (≠ E58), E61 (≠ Q71), H86 (≠ Q95), E134 (= E145)
binding cobalt (ii) ion: H14 (= H22), H86 (≠ Q95), E134 (= E145)

A1YPR3 2-epi-5-epi-valiolone epimerase; EVE; EC 5.1.3.33 from Actinomyces sp. (see paper)
32% identity, 72% coverage: 11:120/153 of query aligns to 5:125/183 of A1YPR3

query
sites
A1YPR3

G

G

V

I

P

P

G

G

H

A

L

V

F

-

T

-

A

A

I

V

D

H

H
|
H

V

V

G

A

M

Y

A

T

V

V

P

P

D

D

L

L

D

D

V

Q

A

A

I

V

A

E

F

F

Y

F

R

T

D

E

T

V

F

I

G

G

M

A

T

E

L

L

A

A

H

Y

E

T

E

L

V

V

N

Q

E

D

E

A

Q

A

G

G

-

D

-

W

-

M

-

T

-

R

-

K

-

L

-

D

-

V

-

D

-

A

-

T

-

A

-

T

V

A

R

R

E

I

A

A

M

M

M

L

A

R

V

L

G

G

D

P

S

V

T

T

S

N

C

-

V

L

Q
x
E

L

L

L

F

A

E

P

Y

L

A

S

A

P

P

E

D

S

Q

T

R

I

-

A

-

K

R

F

Q

I

L

D

P

R

R

N

N

G

S

-

D

P

W

G

G

C

G

Q

H

Q
x
H

I

L

A

A

Y

I

R

H

V

V

T

A

D

D

V

V

D

D

A

A

V

A

S

A

A

E

T

Y

L

L

R

R

E

A

R

Q

-

P

G

G

L

V

R

R

M

V

L

L

Y

G

D

D

H14 (= H22) mutation to G: Lack of activity; when associated with G-76; G-99 or G-151.
E76 (≠ Q71) mutation to G: Lack of activity; when associated with G-14; G-99 or G-151.
H99 (≠ Q95) mutation to G: Lack of activity; when associated with G-14; G-76 or G-151.

Sites not aligning to the query:

151 E→G: Lack of activity; when associated with G-14; G-76 or G-99.

Query Sequence

>WP_068115344.1 NCBI__GCF_001653335.1:WP_068115344.1
MTSSAAPGPLGVPGHLFTAIDHVGMAVPDLDVAIAFYRDTFGMTLAHEEVNEEQGVREAM
MAVGDSTSCVQLLAPLSPESTIAKFIDRNGPGCQQIAYRVTDVDAVSATLRERGLRMLYD
EPRRGTSDSRINFVHPKDAGGVLVEIVEPAAHH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory