SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_068166475.1 NCBI__GCF_001592305.1:WP_068166475.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
25% identity, 79% coverage: 47:498/575 of query aligns to 11:443/502 of P07117

query
sites
P07117

F

F

A

C

L

V

F

Y

V

I

V

F

G

G

T

M

L

I

F

L

I

I

T

G

K

F

W

I

A

A

A

W

A

R

K

S

T

T

K

K

S

N

A

F

S

D

D

D

F

Y

Y

I

T

L

A

G

G

G

G

R

G

S

I

L

T

G

G

P

F

F

Q

V

N

T

G

A

L

L

A

S

I

A

A

G

G

A

D

S

Y

D

M

M

S

S

A

G

A

W

S

L

F

L

L

M

G

G

I

L

S

P

A

G

A

A

V

V

M

F

A

L

S

S

G

G

F

I

D

S

G

E

L

S

I

W

Y

I

S

A

I

I

G

G

F

L

L

T

V

L

G

G

W

A

P

W

V

I

I

N

T

W

F

K

L

L

M

V

A

A

E

G

R

R

L

L

R

R

-

V

-

H

-

T

-

E

-

Y

N

N

L

N

G

N

K

A

F

L

T

T

F

L

A

P

D

D

V

Y

A

F

A

T

F

G

R

R

F

F

N

E

Q

D

T

K

P

S

-

R

-

I

I

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R

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I

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A

A

S

L

G

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I

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F

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Y

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C

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M

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G

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A

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G

A

Q

R

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I

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K

E

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F

F

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M

E

S

Y

Y

Y

E

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A

A

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I

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G

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A

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M

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L

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F

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G

G

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F

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A

A

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W

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D

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Q

A

A

C

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L

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G

A

A

L

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F

L

M

T

A

P

L

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I

V

V

L

I

W

I

H

S

F

V

G

G

F

-

S

-

P

-

E

-

A

-

M

G

F

F

A

G

S

D

A

S

V

L

Q

E

I

V

K

-

T

-

D

-

L

-

A

-

L

I

K

K

D

Q

G

K

K

S

I

I

A

E

E

N

A

V

A

D

T

M

A

L

A

K

G

G

Q

L

S

-

I

-

M

-

G

-

P

-

G

-

N

N

F

F

V

V

K

A

D

I

P

-

I

I

S

S

A

L

I

M

S

G

F

W

G

G

M

L

A

-

L

-

M

-

F

-

G

G

T

Y

A

F

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G

L

Q

P

P

H

H

I

I

L

L

M

A

R
|
R

F

F

F

M

T

-

V

-

P

-

S

-

A

A

K

A

E

D

A

S

R

H

K

H

S

S

V

I

M

V

W

H

A

A

T

R

G

R

W

-

I

I

G

S

Y

M

F

T

Y

W

L

M

L

I

T

L

F
x
C

I

L

I

A

G

G

F

A

G

V

A

A

I

V

T

G

F

F

V

F

L

G

I

I

N

-

P

-

Q

A

F

Y

L

F

D

N

A

D

K

H

G

P

G

A

L

L

L

A

G

G

G

A

N

V

N

N

M

Q

A

N

A

A

V

E

H

R

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A

F

N

I

A

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G

A

G

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N

I

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P

F

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I

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A

A

G

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I

A

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F

L

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A

I

I

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L

A

A

V

A

V

V

A

M

G

S

L

-

T

T

L

L

S

S

-
x
C

-

Q

-

L

-

L

-

V

G
x
C

A

S

S

S

A

A

V

I

S

T

H

E

D

D

L

L

Y

Y

A

K

T

A

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F

I

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K

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K

K

G

-

Q

H

A

A

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S

S

Q

A

K

S

E

E

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L

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W

V

V

S

G

K
x
R

A

V

T

M

T

V

I

L

A

V

L

V

G

A

I

L

I

V

A

A

V

I

V

A

L

L

G

A

I

A

A

N

F

P

E

E

K

N

Q

R

N

V

I

L

A

G

F

-

M

L

V

V

S

S

L

Y

A

A

F

W

A

A

I

G

A

F

A

G

S

A

A

A

N

F

F

G

P

P

V

V

L

V

F

L

M

F

S

S

V

V

L

M

W

W

K

S

D

R

C

M

T

T

T

R

K

N

G

G

A

A

V

L

I

A

G

G

G

M

F

I

L

I

G

G

L

A

I

L

S

T

S

V

V

I

V

V

R257 (= R307) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ F336) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (vs. gap) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ G402) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (≠ K430) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
23% identity, 32% coverage: 45:226/575 of query aligns to 18:201/643 of Q92911

query
sites
Q92911

G

G

M

V

F

F

A

A

L

L

F

M

V

L

V

L

G

V

T

S

L

T

F

G

I

I

T

G

K

L

W

W

-

V

-

G

-

L

A

A

A

R

A

G

K

G

T

Q

K

R

S

S

A

A

S

E

D

D

F

F

Y

F

T

T

A

G

G

G

G

R

G

R

I

L

T

A

G

A

F

L

Q

P

N

V

G

G

L

L

A

S

I

L

A

S

G

A

D

S

Y

F

M

M

S

S

A

A

A

V

S

Q

F

V

L

L

G

G

I

V

S

P

A

S

A

E

V

A

M

Y

A

R

S

Y

G

G

F

L

D

K

G

F

L

L

I

W

Y

M

S

C

I

L

G

G

F

Q

L

L

V

L

G

N

W

S

P

V

V

L

I

T

T
x
A

F

L

L

L

M

F

A

M

E

P

R

V

L

F

R

Y

N

R

L

L

G

G

K

L

F

T

T

S

F

T

A

Y

D

E

V

Y

A

L

A

E

F

M

R

R

F

F

N

S

Q

R

T

A

P

-

I

V

R

R

I

L

F

C

A

G

A

T

S

L

G

Q

T

Y

L

I

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V

V

A

V

T

A

M

F

L

Y

Y

L

T

I

G

A

I

Q

V

M

I

V

Y

G

A

A

P

G

A

Q

L

L

I

I

L

K

N

L

Q

L

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F

T

G

G

L

L

E

D

Y

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Y

W

I

A

A

S

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L

I

L

I

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T

G

G

A

I

L

I

M

C

M

T

V

F

Y

Y

V

T

L

A

F

V

G

G

G

G

M

M

T

K

A

A

T

V

T

V

W

W

V

T

Q

D

I

V

I

F

K

Q

A

V

C

V

L

V

L

M

L

L

G

S

G

G

A102 (≠ T126) natural variant: A -> P

Sites not aligning to the query:

226 mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
237 D→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

B4EZY7 Sodium/sialic acid symporter SiaT; Na(+)-coupled sialic acid symporter; Sialic acid transporter from Proteus mirabilis (strain HI4320) (see paper)
18% identity, 54% coverage: 48:360/575 of query aligns to 20:316/496 of B4EZY7

query
sites
B4EZY7

A

A

L

M

F

L

V

L

V

V

G

G

T

V

L

Y

F

F

I

-

T

-

K

-

W

-

A

-

A

S

A

K

K

R

T

Q

K

K

S

T

A

A

S

D

D

D

F

Y

Y

F

T

R

A

G

G

G

G

G

G

R

I

V

T

P

G

G

F

W

Q

A

N

A

G

G

L

V

A

S

I

V

A

F

G
x
A

D

T

Y
x
T

M
x
L

S
|
S

A

S

A

I

S
x
T

F

F

L

M

G

S

I

I

S

P

A

A

A

K

V

A

M

Y

A

T

S

S

G

D

F

W

D

T

G

F

L

I

I

I

Y

G

S
x
Q

I

Y

G

L

F

A

L

I

V

A

G

I

W

L

P

P

V

L

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V

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F

F

Y

L

F

M

Y

A

I

E

P

R

F

L

F

R

R

N

K

L

L

G

K

K

I

F

T

T

S

F

A

A

Y

D

E

V

Y

A

L

A

E

F

A

R

R

F

F

N

D

Q

V

T

R

P

S

I

-

R

R

I

L

F

F

A

A

A

S

-

L

-

S

-

F

-

M

-

L

-

F

-

H

S

I

G

G

T
x
R

L

V

V

A

V

I

V

I

A

T

F

Y

Y

L

L

T

I

V

A

L

Q

A

M

L

V

-

G

-

A

-

G

-

Q

-

L

-

I

-

K

R

L

P

L

F

F

M

G

G

L

I

E

D

Y

P

Y

V

I

V

A

L

V

I

I

V

I

L

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I

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S

A

L

L

L

M

C

M

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I

Y

Y

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T

L

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F

M

G

G

G

G

M

I

T

E

A

G

T

V

T

I

W

W

V

T

Q
x
D

I

V

I

I

K

Q

A

G

C

L

L

L

L

L

L

S

G

G

G

G

A

A

T

V

F

L

M

I

A

F

L

I

S

M

V

I

L

C

W

F

H

K

F

V

G

D

F

G

S

G

P

I

E

S

A

E

M

I

F

F

A

T

S

T

A

T

V

A

Q

Q

I

A

K

D

T

K

D

F

L

F

A

P

L

T

K

T

D

Q

G

W

K

R

I

W

A

S

E

W

A

T

A

D

T

S

A

T

A

I

G

-

Q

-

S

-

I

-

M

-

G

-

P

-

G

-

N

-

F

-

V

-

K

-

D

-

P

P

I

V

S

L

A

M

I

I

S

G

F

F

G

L

M

F

A

A

L

N

M

I

F

Q

G

Q

T

F

A

T

G

A

L

S

P

Q

H

D

I

V

L

V

M

Q

R

R

F

Y

F

I

T

V

V

T

P

D

S

S

A

I

K

K

E

E

A

T

R

K

K

R

S

T

V

L

M

I

W

T

A

N

T

A

G

K

W

L

I

V

G

A

Y

I

F

I

Y

P

L

I

L

F

T

F

F

F

I

A

I

I

G

G

F

S

G

A

A

L

I

F

T

V

F

Y

V

Y

L

Q

I

Q

N

N

P

P

Q

S

F

L

L

L

D

P

A

A

-

G

-

F

-

N

K

T

G

G

G

G

L

I

L

L

A56 (≠ G89) binding Na(+)
T58 (≠ Y91) mutation to A: 2-fold increase in Neu5Ac transport.
L59 (≠ M92) binding Na(+)
S60 (= S93) mutation to A: Abolishes Neu5Ac transport.
T63 (≠ S96) mutation to A: Abolishes Neu5Ac transport.
Q82 (≠ S115) mutation to D: Abolishes Neu5Ac transport.
R135 (≠ T162) mutation to E: Abolishes Neu5Ac transport.
D182 (≠ Q216) binding Na(+); mutation to A: Abolishes Neu5Ac transport.

Sites not aligning to the query:

339 binding Na(+)
342 binding Na(+); binding Na(+); S→A: Abolishes Neu5Ac transport.
343 binding Na(+); S→A: Abolishes Neu5Ac transport.
345 binding Na(+); S→A: Reduces Neu5Ac transport.
346 binding Na(+); S→A: Slightly increases Neu5Ac transport.

5nv9A Substrate-bound outward-open state of a na+-coupled sialic acid symporter reveals a novel na+-site (see paper)
18% identity, 54% coverage: 48:360/575 of query aligns to 16:312/480 of 5nv9A

query
sites
5nv9A

A

A

L

M

F

L

V

L

V

V

G

G

T

V

L

Y

F

F

I

-

T

-

K

-

W

-

A

-

A

S

A

K

K

R

T

Q

K

K

S

T

A

A

S

D

D

D

F

Y

Y

F

T

R

A

G

G

G

G

G

G

R

I

V

T

P

G

G

F

W

Q

A

N

A

G

G

L

V

A

S

I

V

A

F

G
x
A

D
x
T

Y
x
T

M
x
L

S
|
S

A

S

A
x
I

S
x
T

F

F

L

M

G

S

I

I

S

P

A

A

A

K

V

A

M

Y

A

T

S

S

G

D

F

W

D

T

G

F

L

I

I

I

Y
x
G

S
x
Q

I

Y

G

L

F

A

L

I

V

A

G

I

W

L

P

P

V

L

I

V

T

F

F

Y

L

F

M

Y

A

I

E

P

R

F

L

F

R

R

N

K

L

L

G

K

K

I

F

T

T

S

F

A

A

Y

D

E

V

Y

A

L

A

E

F

A

R

R

F

F

N

D

Q

V

T

R

P

S

I

-

R

R

I

L

F

F

A

A

A

S

-

L

-

S

-

F

-

M

-

L

-

F

-

H

S

I

G

G

T
x
R

L

V

V

A

V

I

V

I

A

T

F

Y

Y

L

L

T

I

V

A

L

Q

A

M

L

V

-

G

-

A

-

G

-

Q

-

L

-

I

-

K

R

L

P

L

F

F

M

G

G

L

I

E

D

Y

P

Y

V

I

V

A

L

V

I

I

V

I

L

V

I

G

S

A

L

L

L

M

C

M

I

V

I

Y

Y

V

T

L

W

F

M

G

G

G

G

M

I

T

E

A

G

T
x
V

T

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W

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x
D

I

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A

G

C

L

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S

G

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A

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Q

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|
F

G

L

M

F

A

A

L

N

M

I

F

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G

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-

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L

binding sodium ion: A52 (≠ G89), T53 (≠ D90), L55 (≠ M92), S56 (= S93), V174 (≠ T212), D178 (≠ Q216)
binding N-acetyl-beta-neuraminic acid: T54 (≠ Y91), S56 (= S93), I58 (≠ A95), T59 (≠ S96), G77 (≠ Y114), Q78 (≠ S115), R131 (≠ T162), F239 (= F290)

Sites not aligning to the query:

binding sodium ion: 335, 338, 338, 339, 341, 342

Query Sequence

>WP_068166475.1 NCBI__GCF_001592305.1:WP_068166475.1
MNFLHAHTRRLLTLLALFGVSAVAWAAGADLGQAEKQATNWTAIGMFALFVVGTLFITKW
AAAKTKSASDFYTAGGGITGFQNGLAIAGDYMSAASFLGISAAVMASGFDGLIYSIGFLV
GWPVITFLMAERLRNLGKFTFADVAAFRFNQTPIRIFAASGTLVVVAFYLIAQMVGAGQL
IKLLFGLEYYIAVIIVGALMMVYVLFGGMTATTWVQIIKACLLLGGATFMALSVLWHFGF
SPEAMFASAVQIKTDLALKDGKIAEAATAAGQSIMGPGNFVKDPISAISFGMALMFGTAG
LPHILMRFFTVPSAKEARKSVMWATGWIGYFYLLTFIIGFGAITFVLINPQFLDAKGGLL
GGNNMAAVHLANAVGGNVFLGFISAVAFATILAVVAGLTLSGASAVSHDLYATVIKKGQA
DSASELRVSKATTIALGIIAVVLGIAFEKQNIAFMVSLAFAIAASANFPVLFMSVLWKDC
TTKGAVIGGFLGLISSVVLTVVSPSVWEVTLGNPKGSALFPYTSPALFSMTIGFVGIWLF
SILDNSQRAKVDRGGFLAQQVRSETGIGASGASGH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory