SitesBLAST
Comparing WP_068174326.1 NCBI__GCF_001592305.1:WP_068174326.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
46% identity, 94% coverage: 12:652/684 of query aligns to 9:654/692 of 6iunB
- active site: A60 (≠ S63), F65 (= F68), E73 (= E74), H77 (≠ P78), G101 (= G102), E104 (= E105), E124 (= E125), G132 (= G133), K248 (≠ A246), S407 (= S404), H428 (= H425), E440 (= E437), N478 (= N475)
- binding nicotinamide-adenine-dinucleotide: G300 (= G297), T301 (= T298), M302 (= M299), E321 (= E318), T322 (≠ Q319), Y365 (≠ W362), A377 (= A374), V378 (= V375), E380 (= E377), V384 (= V381), V388 (= V385), N405 (= N402), S407 (= S404)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
39% identity, 96% coverage: 6:660/684 of query aligns to 4:679/723 of Q08426
- V40 (≠ R42) to G: in dbSNP:rs1062551
- I41 (≠ N43) to R: in dbSNP:rs1062552
- T75 (≠ Q76) to I: in dbSNP:rs1062553
- K165 (≠ E166) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ L172) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A270) to T: in dbSNP:rs2302819
- A325 (≠ L316) to G: in dbSNP:rs1062555
- K346 (≠ S337) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (vs. gap) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ A579) to T: in dbSNP:rs1042437
- T606 (≠ D587) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
6z5oAAA Peroxisomal bifunctional enzyme (see paper)
38% identity, 96% coverage: 6:660/684 of query aligns to 10:677/716 of 6z5oAAA
- active site: A67 (≠ S63), F72 (= F68), G82 (≠ P78), G106 (= G102), E109 (= E105), P128 (= P124), E129 (= E125), G137 (= G133), K255 (≠ A246), S409 (= S404), H430 (= H425), E442 (= E437), N480 (= N475)
- binding coenzyme a: P26 (= P22), V27 (≠ I23), A65 (= A61), D68 (= D64), I69 (≠ L65), P128 (= P124), Y162 (≠ R158), F277 (= F268), K281 (≠ R272)
- binding nicotinamide-adenine-dinucleotide: G309 (= G295), G311 (= G297), T312 (= T298), M313 (= M299), E332 (= E318), S333 (≠ Q319), Q337 (≠ A323), A379 (= A374), V380 (= V375), F381 (= F376), E382 (= E377), K387 (= K382), N407 (= N402), S409 (= S404), H430 (= H425)
- binding nicotinamide: A67 (≠ S63), E109 (= E105), E129 (= E125), P136 (= P132), F261 (= F252)
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
38% identity, 96% coverage: 6:660/684 of query aligns to 7:681/723 of 3zw9A
- active site: A64 (≠ S63), F69 (= F68), G79 (≠ P78), G103 (= G102), E106 (= E105), P125 (= P124), E126 (= E125), P133 (= P132), G134 (= G133), K252 (≠ A246), S413 (= S404), H434 (= H425), E446 (= E437), N484 (= N475)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ I294), G306 (= G295), G308 (= G297), T309 (= T298), M310 (= M299), E329 (= E318), Q334 (≠ A323), A383 (= A374), V384 (= V375), F385 (= F376), E386 (= E377), N411 (= N402), S413 (= S404), H434 (= H425)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (≠ I23), A62 (= A61), G63 (= G62), A64 (≠ S63), I66 (≠ L65), G102 (= G101), G103 (= G102), E106 (= E105), E126 (= E125), P133 (= P132), Y159 (≠ R158)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
38% identity, 96% coverage: 6:660/684 of query aligns to 9:683/725 of 5omoA
- active site: A66 (≠ S63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (≠ A246), S415 (= S404), H436 (= H425), E448 (= E437), N486 (= N475)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P22), V26 (≠ I23), A28 (= A25), P31 (≠ A28), A64 (= A61), A66 (≠ S63), D67 (= D64), I68 (≠ L65), L103 (≠ Q100), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), Y161 (≠ R158), F260 (= F252), K280 (≠ R272)
- binding 3-keto-decanoyl-coa: S415 (= S404), N486 (= N475), K519 (≠ A508), M520 (= M509), V525 (= V514), Y658 (= Y635)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
38% identity, 96% coverage: 6:660/684 of query aligns to 9:683/725 of 5mgbA
- active site: A66 (≠ S63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (≠ A246), S415 (= S404), H436 (= H425), E448 (= E437), N486 (= N475)
- binding acetoacetyl-coenzyme a: P25 (= P22), V26 (≠ I23), A64 (= A61), G65 (= G62), A66 (≠ S63), D67 (= D64), I68 (≠ L65), G105 (= G102), E128 (= E125), Y161 (≠ R158)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I294), G308 (= G295), G310 (= G297), T311 (= T298), M312 (= M299), E331 (= E318), S332 (≠ Q319), Q336 (≠ A323), V386 (= V375), F387 (= F376), E388 (= E377), N413 (= N402), S415 (= S404), H436 (= H425)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
38% identity, 96% coverage: 6:660/684 of query aligns to 9:683/725 of 3zwcA