SitesBLAST

H247 (= H164) binding Zn(2+)
C257 (= C174) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
C259 (= C176) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
C264 (= C181) binding Zn(2+)
R306 (≠ E227) mutation to G: Forms dimers but not tetramers; when associated with G-310.
L310 (≠ I231) mutation to G: Forms dimers but not tetramers; when associated with G-306.

Sites not aligning to the query:

52 R→H: Shows increased expression and forms larger colonies.
86 H→R: Can be bound and inactivated by MtfA.
136 F→A: Decreases association with PtsG EIIB domain.

1z6rA Crystal structure of mlc from escherichia coli (see paper)
28% identity, 67% coverage: 93:313/332 of query aligns to 152:366/382 of 1z6rA

query
sites
1z6rA

V

M

P

P

F

L

I

G

D

E

M

A

F

L

K

E

E

Q

Y

H

F

T

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G

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P

P

M

V

V

Y

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N

H

D

D

A

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S

A

A

A

W

A

T

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M

G

A

E

E

K

A

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F

F

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G

G

A

A

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M

A

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D

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H

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C

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C

L

L

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E

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T

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A

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-

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N

R

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-

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A

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G

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D

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A

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M

A

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Q

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K

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L

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K

A

A

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A

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D

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A

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Y

Q

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A

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A

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G

Q

K

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T

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Q

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F

M

-

D

-

R

S

N

N

A

Q

A

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M

G

A

A

G

S

A

A

A

L

L

V

V

binding zinc ion: H223 (= H164), C233 (= C174), C235 (= C176), C240 (= C181)

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
28% identity, 79% coverage: 4:266/332 of query aligns to 408:672/722 of O35826

query
sites
O35826

T
x
S

A
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A

I
x
L

G
x
A

I
x
V

D
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D

I
x
L

G
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G

T
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T

F
x
N

T
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K
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R

F
x
V

G
x
A

L
x
I

T
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M

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K

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G

N
x
E

V
x
I

L

-

M

-

E

-

G

-

S

-

I
x
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Y
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Y

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T

H
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Q

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E
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N

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K

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T

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S

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A
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I
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E
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M

S
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C

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V

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E
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A

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E
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E

P
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A

I
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E
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K

-
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-
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N

-
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C

-
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I

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G

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V

G
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G

I
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I

G
x
S

A
x
T

P
x
G

-
x
G

N
x
R

G
x
V

N
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N

Y
x
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Y
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Q

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G
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G

T
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I
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x
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H

A
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P
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L

-
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Q

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W
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W

K
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-
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M
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L

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P

M
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V
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W

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T
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D

N
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N

D
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D

A
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G

N
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N

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C

A
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A

I
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M

G
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E

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F

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A
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K

D
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F

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G

D
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P
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A

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A
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L

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K
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G

L
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V

A
x
V

D
x
N

A
x
I

A
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L

A
x
H

V
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T
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M

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A

D413 (= D9) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
R420 (≠ K16) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 18 papers)
28% identity, 79% coverage: 4:266/332 of query aligns to 408:672/722 of Q9Y223

query
sites
Q9Y223

T

S

A

A

I

L

G

A

I

V

D
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D

I

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G

G

G
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G

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M

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C

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G

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G

G

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I

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A

T

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G

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x
G

N
x
R

G

V

N

N

Y

P

Y

R

K

E

G

G

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I

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H

A

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P
x
T

N

K

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L

-

I

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Q

H

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W

W

K

N

G

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D

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M

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E

-

Y

-

F

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D

-

L

L

P

P

M

V

V

W

L

V

T

D

N
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N

D
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D

A

G

N
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N

A

C

A

A

I

L

G
x
A

E

E

K

R

V

K

F
|
F

G

G

G

Q

A

G

K

K

D

G

M

L

D

E

D

N

F

F

I

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V

T

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T

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G

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T

G
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G

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G

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G

G

G

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A

A

G

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E

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G

H
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H

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V

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D

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G

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C

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C

G

G

K

S

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H

G

G

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C

L
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E

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Y

A

A

S

S

A

G

S

M

G

A

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R

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A

F

K

K

K

-

L

-

H

-

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-

E

-

D

L

L

A

L

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V

N

E

N

G

V

M

D

S

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P

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R

-

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-

Y

-

T

K

Y

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E

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G

S

A

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K

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M
x
A

A
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A

L

K

E

L

G

G

D

N

P

A

I

K

A

A

L

Q

E

S

A

I

Y

L

T

R

Y

T

T

A

S

G

D

T

I

A

L

L

G

G

L

L

K

G

L

V

A

V

D

N

A

I

A

L

A

H

V

T

T

M

S

N

P

P

Q

S

A

L

I

V

F

I

L

L

F

S

G

G

G

V

L

L

A

A

D413 (= D9) binding Mg(2+)
G416 (= G12) binding an N-acyl-D-mannosamine 6-phosphate
T417 (= T13) binding ADP
N418 (≠ F14) binding ADP
R420 (≠ K16) binding ADP
I472 (= I69) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 50% of the wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity
G476 (vs. gap) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
R477 (≠ N73) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
T489 (≠ P85) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
N516 (= N111) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
D517 (= D112) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (= N114) to S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910; mutation to S: Decreased N-acylmannosamine kinase activity.
A524 (≠ G119) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (= F123) to C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986; mutation to C: Decreased N-acylmannosamine kinase activity.
G545 (= G140) binding an N-acyl-D-mannosamine 6-phosphate
E566 (= E161) binding an N-acyl-D-mannosamine
H569 (= H164) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
V572 (= V167) to L: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70-80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G171) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
C579 (= C174) binding Zn(2+)
C581 (= C176) binding Zn(2+)
C586 (= C181) binding Zn(2+)
I587 (≠ L182) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603; mutation to T: Decreased N-acylmannosamine kinase activity.
E588 (= E183) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
A630 (≠ M224) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
A631 (= A225) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs121908626; to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs62541771; mutation A->V,T: Decreased N-acylmannosamine kinase activity.

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding UDP
23 binding UDP
113 binding UDP
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; impaired homohexamers formation
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 90% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding UDP
253 binding UDP
259 binding CMP-N-acetyl-beta-neuraminate
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding CMP-N-acetyl-beta-neuraminate
280 binding CMP-N-acetyl-beta-neuraminate
281 binding CMP-N-acetyl-beta-neuraminate
282 binding UDP
301 binding UDP
302 binding UDP
303 C → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to 60% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding UDP
321 binding UDP
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 10-30% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs199877522
708 G → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; severely decreased; dbSNP:rs1554657922
712 M→T: Decreased N-acylmannosamine kinase activity.

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
27% identity, 79% coverage: 4:266/332 of query aligns to 4:263/308 of 2yi1A

query
sites
2yi1A

T

S

A

A

I

L

G

A

I

V

D

D

I

L

G
|
G

T
|
T

F
x
N

T

L

K
x
R

F

V

G

A

L

I

T

V

D

S

V

M

E

K

G

G

N

E

V

I

L

-

M

-

E

-

G

-

S

-

I

V

P

K

T

K

Y

Y

T

T

H

Q

K

F

E

N

I

P

K

K

S

T

F

Y

L

E

D

E

A

R

L

I

S

N

Q

L

A

I

I

L

N

Q

E

M

S

C

L

V

D

E

E

A

L

A

N

A

E

E

P

A

I

V

E

K

-

L

-

N

-

C

-

R

I

I

L

L

G

G

V

V

G

G

I

I

G

S

A

T

P
x
G

N
x
G

G
x
R

-

V

N

N

Y

P

Y

R

K

E

G

G

T

I

I

V

E

L

H

H

A
x
S

P
x
T

N

K

L
|
L

-

I

-

Q

H

E

W

W

K

N

G

S

I

-

V

V

P

D

F

L

I

R

D

T

M

P

F

L

K

S

E

D

Y

T

F

L

D

H

L

L

P

P

M

V

V

W

L

V

T

D

N
|
N

D
|
D

A

G

N
|
N

A

C

A

A

I

L

G

A

E

E

K

R

V

K

F

F

G

G

G

Q

A

G

K

K

D

G

M

L

D

E

D

N

F

F

I

V

V

T

I

L

T

I

L

T

G
|
G

T
|
T

G
|
G

L
x
I

G

G

S
x
G

G

G

L

I

V

I

T

H

R

Q

G

H

K

E

L

L

I

I

Y

H

G

G

H

S

D

S

G

F

L

C

A

A

G
x
A

E
|
E

L

L

G

G

H
|
H

T

L

N

V

V

V

Y

S

P

L

D

D

G

G

R

P

E

D

C
|
C

N

S

C
|
C

G

G

K

S

R

H

G

G

C
|
C

L

I

E
|
E

T

A

Y

Y

A

A

S

S

A
x
G

S

M

G

A

I

L

K

Q

R

R

T

E

V

A

F

K

K

K

L

L

L

-

A

-

T

-

N

-

N

H

V

D

D

E

T

D

P

L

L

L

R

L

T

V

Y

E

T

G

Y

M

E

S

S

A

L

V

T

G

S

A

K
x
L

K

H

I

L

T

I

E

Q

M

A

A

A

L

K

E

L

G

G

D

N

P

A

I

K

A

A

L

Q

E

S

A

I

Y

L

T

R

Y

T

T

A

S

G

D

T

I

A

L

L

G

G

L

L

K

G

L

V

A

V

D

N

A

I

A

L

A

H

V

T

T

M

S

N

P

P

Q

S

A

L

I

V

F

I

L

L

F

S

G

G

G
x
V

L

L

A

A

binding adenosine-5'-diphosphate: G11 (= G11), T13 (= T13), N14 (≠ F14), R16 (≠ K16), T140 (= T139), G189 (≠ A188), L216 (≠ K219), V261 (≠ G264)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (= G12), G71 (≠ P72), G72 (≠ N73), R73 (≠ G74), S84 (≠ A84), T85 (≠ P85), L87 (= L87), N112 (= N111), D113 (= D112), G139 (= G138), T140 (= T139), G141 (= G140), I142 (≠ L141), E162 (= E161), H165 (= H164), E184 (= E183)
binding calcium ion: N112 (= N111), N115 (= N114), G144 (≠ S143), A161 (≠ G160)
binding zinc ion: H165 (= H164), C175 (= C174), C177 (= C176), C182 (= C181)

2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
27% identity, 79% coverage: 4:266/332 of query aligns to 4:263/308 of 2yhyA

query
sites
2yhyA

T

S

A

A

I

L

G

A

I

V

D

D

I

L

G
|
G

T
|
T

F
x
N

T

L

K
x
R

F

V

G

A

L

I

T

V

D

S

V

M

E

K

G

G

N

E

V

I

L

-

M

-

E

-

G

-

S

-

I

V

P

K

T

K

Y

Y

T

T

H

Q

K

F

E

N

I

P

K

K

S

T

F

Y

L

E

D

E

A

R

L

I

S

N

Q

L

A

I

I

L

N

Q

E

M

S

C

L

V

D

E

E

A

L

A

N

A

E

E

P

A

I

V

E

K

-

L

-

N

-

C

-

R

I

I

L

L

G

G

V

V

G

G

I

I

G

S

A

T

P

G

N

G

G

R

-

V

N

N

Y

P

Y

R

K

E

G

G

T

I

I

V

E

L

H

H

A

S

P

T

N

K

L

L

-

I

-

Q

H

E

W

W

K

N

G

S

I

-

V

V

P

D

F

L

I

R

D

T

M

P

F

L

K

S

E

D

Y

T

F

L

D

H

L

L

P

P

M

V

V

W

L

V

T

D

N
|
N

D

D

A

G

N
|
N

A

C

A

A

I

L

G

A

E

E

K

R

V

K

F

F

G

G

G

Q

A

G

K

K

D

G

M

L

D

E

D

N

F

F

I

V

V

T

I

L

T

I

L

T

G

G

T
|
T

G

G

L

I

G

G

S
x
G

G

G

L

I

V

I

T

H

R

Q

G

H

K

E

L

L

I

I

Y

H

G

G

H

S

D

S

G

F

L

C

A

A

G
x
A

E

E

L

L

G

G

H
|
H

T

L

N

V

V

V

Y

S

P

L

D

D

G

G

R

P

E

D

C
|
C

N

S

C
|
C

G

G

K

S

R

H

G

G

C
|
C

L

I

E

E

T

A

Y

Y

A

A

S

S

A
x
G

S

M

G

A

I

L

K

Q

R

R

T

E

V

A

F

K

K

K

L

L

L

-

A

-

T

-

N

-

N

H

V

D

D

E

T

D

P

L

L

L

R

L

T

V

Y

E

T

G

Y

M

E

S

S

A

L

V

T

G

S

A

K
x
L

K

H

I

L

T

I

E

Q

M

A

A

A

L

K

E

L

G

G

D

N

P

A

I

K

A

A

L

Q

E

S

A

I

Y

L

T

R

Y

T

T

A

S

G

D

T

I

A

L

L

G

G

L

L

K

G

L

V

A

V

D

N

A

I

A

L

A

H

V

T

T

M

S

N

P

P

Q

S

A

L

I

V

F

I

L

L

F

S

G

G

G
x
V

L

L

A

A

binding adenosine-5'-diphosphate: G11 (= G11), G12 (= G12), T13 (= T13), N14 (≠ F14), R16 (≠ K16), T140 (= T139), G189 (≠ A188), L216 (≠ K219), V261 (≠ G264)
binding calcium ion: N112 (= N111), N115 (= N114), G144 (≠ S143), A161 (≠ G160)
binding zinc ion: H165 (= H164), C175 (= C174), C177 (= C176), C182 (= C181)

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
27% identity, 79% coverage: 4:266/332 of query aligns to 4:264/309 of 2yhwA

query
sites
2yhwA

T

S

A

A

I

L

G

A

I

V

D

D

I

L

G

G

T

T

F

N

T

L

K

R

F

V

G

A

L

I

T

V

D

S

V

M

E

K

G

G

N

E

V

I

L

-

M

-

E

-

G

-

S

-

I

V

P

K

T

K

Y

Y

T

T

H

Q

K

F

E

N

I

P

K

K

S

T

F

Y

L

E

D

E

A

R

L

I

S

N

Q

L

A

I

I

L

N

Q

E

M

S

C

L

V

D

E

E

A

L

A

N

A

E

E

P

A

I

V

E

K

-

L

-

N

-

C

-

R

I

I

L

L

G

G

V

V

G

G

I

I

G

S

A

T

P

G

N

G

G

R

-

V

N

N

Y

P

Y

R

K

E

G

G

T

I

I

V

E

L

H

H

A

S

P

T

N

K

L

L

-

I

-

Q

H

E

W

W

K

N

G

S

I

-

V

V

P

D

F

L

I

R

D

T

M

P

F

L

K

S

E

D

Y

T

F

L

D

H

L

L

P

P

M

V

V

W

L

V

T

D

N
|
N

D

D

A

G

N
|
N

A

C

A

A

I

L

G

A

E

E

K

R

V

K

F

F

G

G

G

Q

A

G

K

K

D

G

M

L

D

E

D

N

F

F

I

V

V

T

I

L

T

I

L

T

G

G

T

T

G

G

L

I

G

G

S
x
G

G

G

L

I

V

I

T

H

R

Q

G

H

K

E

L

L

I

I

Y

H

G

G

H

S

D

S

G

F

L

C

A

A

G
x
A

E

E

L

L

G

G

H
|
H

T

L

N

V

V

V

Y

S

P

L

D

N

G

G

R

P

E

D

C
|
C

N

S

C
|
C

G

G

K

S

R

H

G

G

C
|
C

L

I

E

E

T

A

Y

Y

A

A

S

S

A

G

S

M

G

A

I

L

K

Q

R

R

T

E

V

A

F

K

K

K

L

L

L

-

A

-

T

-

N

H

N

D

V

E

D

D

T

L

P

L

L

L

R

V

T

E

Y

G

T

M

Y

S

E

V

S

A

L

V

T

G

S

A

K

L

K

H

I

L

T

I

E

Q

M

A

A

A

L

K

E

L

G

G

D

N

P

A

I

K

A

A

L

Q

E

S

A

I

Y

L

T

R

Y

T

T

A

S

G

D

T

I

A

L

L

G

G

L

L

K

G

L

V

A

V

D

N

A

I

A

L

A

H

V

T

T

M

S

N

P

P

Q

S

A

L

I

V

F

I

L

L

F

S

G

G

G

V

L

L

A

A

binding calcium ion: N112 (= N111), N115 (= N114), G144 (≠ S143), A161 (≠ G160)
binding zinc ion: H165 (= H164), C175 (= C174), C177 (= C176), C182 (= C181)

3eo3A Crystal structure of the n-acetylmannosamine kinase domain of human gne protein (see paper)
26% identity, 79% coverage: 4:266/332 of query aligns to 3:243/288 of 3eo3A

query
sites
3eo3A

T

S

A

A

I

L

G

A

I

V

D

D

I

L

G

G

T

T

F

N

T

L

K

R

F

V

G

A

L

I

T

V

D

S

V

M

E

K

G

G

N

E

V

I

L

-

M

-

E

-

G

-

S

-

I

V

P

K

T

K

Y

Y

T

T

H

Q

K

F

E

N

I

P

K

K

S

T

F

Y

L

E

D

E

A

R

L

I

S

N

Q

L

A

I

I

L

N

Q

E

M

S

C

L

V

D

E

E

A

L

A

N

A

E

E

P

A

I

V

E

K

-

L

-

N

-

C

-

R

I

I

L

L

G

G

V

V

G

G

I

I

G

S

A

-

P

-

N

-

G

-

N

-

Y

-

K

-

G

-

T

T

I

D

E

L

H

R

A

T

P

P

N

-

L

-

H

-

W

-

K

-

G

-

I

-

V

-

P

-

F

-

I

-

D

-

M

-

F

L

K

S

E

D

Y

T

F

L

D

H

L

L

P

P

M

V

V

W

L

V

T

D

N

N

D

D

A

G

N

N

A

C

A

A

I

L

G

A

E

E

K

R

V

K

F

F

G

G

G

Q

A

G

K

K

D

G

M

L

D

E

D

N

F

F

I

V

V

T

I

L

T

I

L

T

G

G

T

T

G

G

L

I

G

G

S

G

G

G

L

I

V

I

T

H

R

Q

G

H

K

E

L

L

I

I

Y

H

G

G

H

S

D

S

G

F

L

C

A

A

G

A

E

E

L

L

G

G

H
|
H

T

L

N

V

V

V

Y

S

P

L

D

D

G

G

R

P

E

D

C
|
C

N

S

C
|
C

G

G

K

S

R

H

G

G

C
|
C

L

I

E

E

T

A

Y

Y

A

A

S

S

A

G

S

M

G

A

I

L

K

Q

R

R

T

E

V

A

F

K

K

K

-

L

-

H

-

D

-

E

-

D

L

L

A

L

T

V

N

E

N

G

V

M

D

S

T

V

P

P

L

-

R

-

T

-

Y

-

T

K

Y

D

E

E

S

A

L

V

T

G

S

A

K

L

K

H

I

L

T

I

E

Q

M

A

A

A

L

K

E

L

G

G

D

N

P

A

I

K

A

A

L

Q

E

S

A

I

Y

L

T

R

Y

T

T

A

S

G

D

T

I

A

L

L

G

G

L

L

K

G

L

V

A

V

D

N

A

I

A

L

A

H

V

T

T

M

S

N

P

P

Q

S

A

L

I

V

F

I

L

L

F

S

G

G

G

V

L

L

A

A

binding zinc ion: H140 (= H164), C150 (= C174), C152 (= C176), C157 (= C181)

2aa4A Crystal structure of escherichia coli putative n-acetylmannosamine kinase, new york structural genomics consortium
32% identity, 82% coverage: 4:274/332 of query aligns to 2:254/289 of 2aa4A

query
sites
2aa4A

T

T

A

T

I

L

G

A

I

I

D

D

I

I

G

G

T

T

F

K

T

L

K

A

F

A

G

A

L

L

T

I

D

G

V

A

E

D

G

G

N

Q

V

I

L

R

M

D

E

R

G

R

S

E

I

L

P

P

T

T

Y

P

T

A

H

S

K

Q

E

T

I

P

K

E

S

A

F

L

L

R

D

D

A

A

L

L

S

S

Q

-

A

A

I

L

N

V

E

S

S

P

L

L

D

Q

E

A

L

H

N

A

E

Q

P

R

I

V

E

A

I

I

L

A

G

S

V

T

G

G

I

I

G

-

A

-

P

-

N

-

G

-

N

-

Y

I

Y

R

K

D

G

G

T

S

I

L

E

L

H

-

A

A

P

L

N

N

L

P

H

H

W

N

K

L

G

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