SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_068330536.1 NCBI__GCF_001650345.1:WP_068330536.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
79% identity, 98% coverage: 3:498/506 of query aligns to 2:497/502 of P0A6F3

query
sites
P0A6F3

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T14 (= T15) binding ADP; binding sn-glycerol 3-phosphate
R18 (= R19) binding ADP
S59 (= S60) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (= A67) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (= R85) binding glycerol; binding sn-glycerol 3-phosphate
E85 (= E86) binding glycerol; binding sn-glycerol 3-phosphate
Y136 (= Y137) binding glycerol; binding sn-glycerol 3-phosphate
G231 (= G232) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (= K234) modified: N6-malonyllysine
G235 (= G236) binding beta-D-fructose 1,6-bisphosphate
R237 (= R238) binding beta-D-fructose 1,6-bisphosphate; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D247) binding glycerol; binding sn-glycerol 3-phosphate
Q247 (= Q248) binding glycerol
T268 (= T269) binding ADP
G305 (= G306) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G312) binding ADP
G412 (= G413) binding ADP
N416 (= N417) binding ADP
I475 (≠ A476) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (≠ K480) binding Zn(2+)
R480 (= R481) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1glfO Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
79% identity, 98% coverage: 4:498/506 of query aligns to 1:495/498 of 1glfO

query
sites
1glfO

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binding adenosine-5'-diphosphate: R16 (= R19), G265 (= G268), T266 (= T269), G309 (= G312), G410 (= G413), A411 (= A414)
binding glycerol: R82 (= R85), E83 (= E86), Y134 (= Y137), D244 (= D247)
binding phosphate ion: G232 (= G235), G233 (= G236), R235 (= R238)

1bo5O Crystal structure of the complex between escherichia coli glycerol kinase and the allosteric regulator fructose 1,6-bisphosphate. (see paper)
79% identity, 98% coverage: 4:498/506 of query aligns to 1:495/498 of 1bo5O

query
sites
1bo5O

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L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

G

G

G

K

K

G
|
G

G
|
G

T

T

R
|
R

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G

G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A

A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G

G

A

A

V

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding 1,6-di-O-phosphono-beta-D-fructofuranose: G232 (= G235), G233 (= G236), R235 (= R238)
binding glycerol: R82 (= R85), E83 (= E86), W102 (= W105), Y134 (= Y137), D244 (= D247), Q245 (= Q248), F269 (= F272)

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
79% identity, 98% coverage: 4:498/506 of query aligns to 1:495/499 of 1bu6Y

query
sites
1bu6Y

E

E

Q

K

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

S

T

T

L

L

V

V

E

E

A

V

L

L

A

T

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q
|
Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

G

G

G

K

K

G

G

G

G

T

T

R

R

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G

G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A

A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G

G

A

A

V

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding glycerol: Q81 (= Q84), R82 (= R85), E83 (= E86), W102 (= W105), Y134 (= Y137), D244 (= D247)

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
78% identity, 98% coverage: 4:498/506 of query aligns to 1:491/494 of 1gllO

query
sites
1gllO

E

E

Q

K

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

W

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

G

G

-

K

-

G

-

G

-

T

T

R

R

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding phosphomethylphosphonic acid adenylate ester: T12 (= T15), T13 (= T16), G261 (= G268), T262 (= T269), G305 (= G312), I308 (= I315), Q309 (= Q316), A321 (= A328), G406 (= G413), N410 (= N417)
binding glycerol: R82 (= R85), E83 (= E86), Y134 (= Y137), D240 (= D247), Q241 (= Q248), F265 (= F272)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
78% identity, 98% coverage: 4:498/506 of query aligns to 1:491/494 of 1gljO

query
sites
1gljO

E

E

Q

K

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

W

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

G

G

-

K

-

G

-

G

-

T

T

R

R

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I

I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T15), T13 (= T16), G261 (= G268), T262 (= T269), G305 (= G312), Q309 (= Q316), A321 (= A328), G406 (= G413), A407 (= A414)
binding glycerol: R82 (= R85), E83 (= E86), W102 (= W105), Y134 (= Y137), D240 (= D247), F265 (= F272)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
78% identity, 98% coverage: 4:498/506 of query aligns to 1:491/494 of 1bwfO

query
sites
1bwfO

E

E

Q

K

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

W

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

G

G

-

K

-

G

-

G

-

T

T

R

R

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T15), T13 (= T16), T262 (= T269), G305 (= G312), I308 (= I315), Q309 (= Q316), A321 (= A328), G406 (= G413), N410 (= N417)
binding glycerol: R82 (= R85), E83 (= E86), W102 (= W105), Y134 (= Y137), D240 (= D247), Q241 (= Q248), F265 (= F272)

1gldG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
77% identity, 97% coverage: 6:498/506 of query aligns to 1:486/489 of 1gldG

query
sites
1gldG

K

K

Y

Y

I

I

V

V

A

A

L

L

D
|
D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

S

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

-

G

-

K

-

G

-

G

-

T

-

R

-

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding adenosine-5'-diphosphate: R14 (= R19), G256 (= G268), T257 (= T269), G300 (= G312), A316 (= A328), G401 (= G413), A402 (= A414), N405 (= N417)
binding glyceraldehyde-3-phosphate: T10 (= T15), R80 (= R85), E81 (= E86), Y132 (= Y137), D235 (= D247), F260 (= F272)
binding manganese (ii) ion: D7 (= D12), R14 (= R19)

1glcG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
77% identity, 97% coverage: 6:498/506 of query aligns to 1:486/489 of 1glcG

query
sites
1glcG

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

S

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

-

G

-

K

-

G

-

G

-

T

-

R

-

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding adenosine-5'-diphosphate: G256 (= G268), T257 (= T269), G300 (= G312), A316 (= A328), G401 (= G413), A402 (= A414), N405 (= N417)
binding glyceraldehyde-3-phosphate: T10 (= T15), R80 (= R85), E81 (= E86), W100 (= W105), Y132 (= Y137), D235 (= D247), F260 (= F272)

1glbG Structure of the regulatory complex of escherichia coli iiiglc with glycerol kinase (see paper)
77% identity, 97% coverage: 6:498/506 of query aligns to 1:486/489 of 1glbG

query
sites
1glbG

K

K

Y

Y

I

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R
|
R

A

A

V

V

V

V

L

M

D

D

H

H

N

D

A

A

N

N

I

I

V

I

C

S

T

V

A

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

I

I

Y

Y

P

P

K

K

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

M

E

E

I

I

Y

W

A

A

T

T

Q

Q

S

S

S

S

T

T

L

L

V

V

E

E

A

V

L

L

A

A

K

K

A

A

G

D

I

I

R

S

S

S

D

D

Q

Q

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

K

K

N

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

I

C

C

E

E

E

H

L

L

K

K

K

R

Q

D

D

G

L

L

E

E

E

D

Y

Y

I

I

R

R

D

S

N

N

T

T

G

G

L

L

V

V

V

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

S

R

R

E

E

E

R

A

A

E

R

A

R

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

M

T

T

Q

Q

G

G

R

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

T

L

L

Q

D

W

W

D

D

E

D

K

K

L

M

L

L

K

E

A

V

L

L

D

D

I

I

P

P

L

R

S

E

M

M

M

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Q

T

T

N

N

I

I

G

-

G

-

K

-

G

-

G

-

T

-

R

-

I

I

P

P

I

I

S

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

V

V

E

K

Q

E

G

G

Q

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

K

E

D

K

K

A

V

V

T

K

S

S

H

E

N

N

G

G

L

L

L

L

T

T

T

T

L

I

A

A

C

C

G

G

P

P

K

T

G

G

D

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

A

A

V

V

F

F

M

M

G

A

G
|
G

A

A

S

S

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

E

V

M

K

K

L

L

L

I

S

N

D

D

A
|
A

S

Y

D

D

S

S

E

E

Y

Y

F

F

A

A

E

T

K

K

V

V

G

Q

S

N

A

T

N

N

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

Y

Y

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

V

N

N

S

A

N

N

H

H

I

I

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

I

A

A

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

L

D

E

A

A

M

M

Q

Q

A

A

D

D

S

S

G

G

I

I

K

R

L

L

A

H

N

A

L

L

R

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

T

T

E

R

V

V

H

E

R

R

P

P

V

E

V

V

T

R

E

E

V

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

Q

S

N

I

L

D

D

E

E

L

L

K

Q

D

E

K

K

A

A

V

V

L

I

D

E

R

R

S

E

F

F

E

R

P

P

C

G

A

I

N

E

D

T

K

T

K

E

R

R

D

N

R

Y

R

R

Y

Y

R

A

G

G

W

W

Q

K

R

K

A

A

V

V

E

K

C

R

A

A

K

M

G

A

W

W

binding adenosine-5'-diphosphate: R14 (= R19), G256 (= G268), T257 (= T269), G300 (= G312), I303 (= I315), A316 (= A328), G401 (= G413), A402 (= A414), N405 (= N417)
binding glycerol: R80 (= R85), E81 (= E86), W100 (= W105), Y132 (= Y137), D235 (= D247), F260 (= F272)

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
61% identity, 97% coverage: 5:495/506 of query aligns to 2:492/496 of P18157

query
sites
P18157

Q

E

K

T

Y

Y

I

I

V

L

A

S

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

V

I

V

L

L

F

D

N

H

K

N

E

A

G

N

K

I

I

V

V

C

H

T

S

A

A

Q

Q

R

K

E

E

F

F

T

T

Q

Q

I

Y

Y

F

P

P

K

H

A

P

G

G

W

W

V

V

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E

H

H

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N

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Y

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K

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G

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I

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D

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Q

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A

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G

I

I

G

G

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N

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Q

R

R

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T

T

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V

V

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W

D

D

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K

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G

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W

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N

N

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H

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C

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N

N

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F

L

L

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M

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F

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D

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W

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L

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D

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F

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P

C

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D

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K

E

K

K

R

R

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R

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G

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W

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Q

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A

H230 (≠ G233) mutation to R: Increased activity.
F232 (≠ G235) mutation to S: Increased activity.

3ge1A 2.7 angstrom crystal structure of glycerol kinase (glpk) from staphylococcus aureus in complex with adp and glycerol
59% identity, 97% coverage: 5:496/506 of query aligns to 3:494/499 of 3ge1A

query
sites
3ge1A

Q

E

K

K

Y

Y

I

I

V

L

A

S

L

I

D

D

Q

Q

G

G

T

T

T

T

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S

S

S

R

R

A

A

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L

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N

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Q

R

R

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F

T

K

Q

Q

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Y

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P

P

K

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A

S

G

G

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W

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V

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E

H

H

D

D

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A

L

N

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E

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I

Y

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A

T

T

S

Q

V

S

L

S

A

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L

M

V

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A

N

K

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D

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R

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A

D

D

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Q

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A

A

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G

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I

G

G

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I

T

T

N

N

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Q

R

R

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E

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T

T

T

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V

V

V

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W

D

D

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K

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G

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R

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P

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D

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P

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Y

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F

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G

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T

K

K

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K

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W

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L

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D

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N

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V

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G

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A

R

R

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E

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A

A

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G

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L

L

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D

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W

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G

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A

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A

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H

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D

D

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Y

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N

N

A

A

S

S

R

R

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T

M

L

V

M

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F

N

N

I

I

N

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T

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L

L

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W

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D

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L

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M

M

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K

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S

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V

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Y

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G

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K

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R

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G

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D

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C

V

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A

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K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

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G

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D

D

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K

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L

L

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G

A

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V

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F

F

M

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S

G
|
G

A

S

S

A

I
|
I

Q
|
Q

W

W

L

L

R

R

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D

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V

L

K

R

L

M

L

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S

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A
|
A

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P

D

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L

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R

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V

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D

G

G

G
|
G

A
|
A

V

V

A

K

N
|
N

N

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

V

N

N

T

T

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V

V

H

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R

R

P

P

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A

L

L

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G

A

A

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F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

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S

S

I

K

D

D

E

D

L

I

K

A

D

K

K

N

A

W

V

K

L

L

D

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R

E

S

K

F

F

E

D

P

P

C

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A

M

N

D

D

E

K

G

K

E

R

R

D

E

R

K

R

L

Y

Y

R

R

G

G

W

W

Q

K

R

K

A

A

V

V

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E

C

A

A

T

K

Q

binding adenosine-5'-diphosphate: G266 (= G268), T267 (= T269), G310 (= G312), I313 (= I315), Q314 (= Q316), A326 (= A328), P327 (≠ S329), G411 (= G413), A412 (= A414), N415 (= N417)

Q5HGD2 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Staphylococcus aureus (strain COL)
59% identity, 97% coverage: 5:496/506 of query aligns to 2:493/498 of Q5HGD2

query
sites
Q5HGD2

Q

E

K

K

Y

Y

I

I

V

L

A

S

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

A

A

V

I

V

L

L

F

D

N

H

Q

N

K

A

G

N

E

I

I

V

A

C

G

T

V

A

A

Q

Q

R

R

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F

F

T

K

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Q

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Y

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P

P

K

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A

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G

G

W

W

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V

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H

H

D

D

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A

L

N

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W

A

T

T

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L

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A

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L

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A

N

K

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A

N

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D

I

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R

R

S

A

D

D

Q

Q

V

I

A

A

G

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

K

K

N

H

T

T

G

G

K

R

P

P

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Y

Y

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H

A

A

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W

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C

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L

L

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K

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R

R

D

D

N

K

T

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G

G

L

L

V

L

V

L

D

D

P

P

Y
|
Y

F

F

S

A

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

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G

A

A

R

R

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E

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K

A

A

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A

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G

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L

L

L

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W

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L

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W

W

K

K

M

L

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G

K

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A

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H

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D

Y

Y

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N

N

A

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M

L

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M

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F

N

N

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L

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W

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D

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A

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G

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A

A

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G

D
|
D

Q
|
Q

Q

Q

A

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L

L

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Q

M

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C

C

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G

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A

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K

N

N

T

T

Y

Y

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G

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|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

T

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G

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D

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K

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|
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|
Q

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W

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L

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E

G

G

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

P

S

Y

E

A

A

R

R

G

G

A

A

I

I

V

F

G

G

L

L

T

T

R

R

G

G

V

T

N

E

S

K

N

E

H

H

I

F

I

I

R

R

A

A

T

T

L

L

E

E

S

S

V

L

A

C

Y

Y

Q

Q

T

T

R

R

D

D

V

V

L

M

D

E

A

A

M

M

Q

S

A

K

D

D

S

S

G

G

I

I

K

D

L

V

A

Q

N

S

L

L

R

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

K

N
|
N

N

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

V

N

N

T

T

E

S

V

V

H

E

R

R

P

P

V

E

V

I

T

Q

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

E

S

S

I

K

D

D

E

D

L

I

K

A

D

K

K

N

A

W

V

K

L

L

D

E

R

E

S

K

F

F

E

D

P

P

C

K

A

M

N

D

D

E

K

G

K

E

R

R

D

E

R

K

R

L

Y

Y

R

R

G

G

W

W

Q

K

R

K

A

A

V

V

E

E

C

A

A

T

K

Q

T12 (= T15) binding ADP
R16 (= R19) binding ADP
R82 (= R85) binding glycerol
E83 (= E86) binding glycerol
Y134 (= Y137) binding glycerol
D244 (= D247) binding glycerol
Q245 (= Q248) binding glycerol
T266 (= T269) binding ADP
G309 (= G312) binding ADP
Q313 (= Q316) binding ADP
G410 (= G413) binding ADP
N414 (= N417) binding ADP

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
59% identity, 98% coverage: 5:501/506 of query aligns to 2:495/495 of 6udeB

query
sites
6udeB

Q

E

K

K

Y

L

I

I

V

L

A

A

L

L

D

D

Q

Q

G
|
G

T
|
T

T
|
T

S
|
S

S

S

R
|
R

A

A

V

I

V

L

L

F

D

N

H

K

N

S

A

G

N

E

I

I

V

K

C

F

T

V

A

S

Q

Q

R

K

E

S

F

F

T

E

Q

Q

I

I

Y

F

P

P

K

T

A

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

L

N

E

E

I

I

Y

W

A

S

T

S

Q

Q

S

I

S

S

T

V

L

A

V

A

E

E

A

V

L

I

A

A

K

K

A

A

G

G

I

I

R

S

S

G

D

L

Q

E

V

V

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

V

V

W

W

D

D

K

R

N

H

T

T

G

S

K

E

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

D

R

R

R

R

T

T

A

S

D

K

I

Y

C

C

E

D

E

E

L

L

K

K

K

S

Q

Q

D

G

L

H

E

T

E

D

Y

E

I

I

R

K

D

Q

N

K

T

T

G

G

L

L

V

V

V

L

D

D

P

A

Y

Y

F

F

S

S

G

A

T

T

K

K

I

L

K

K

W

W

I

I

L

L

D

D

N

N

V

V

E

E

G

G

A

A

R

R

E

E

E

K

A

A

E

E

A

A

G

G

N

D

L

L

L

C

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

I

I

W

W

K

K

M

L

T

T

Q

R

G

G

R

K

V

M

H

F

V

I

T

T

D

D

Y

V

T

S

N

N

A

A

S

S

R

R

T

T

M

M

V

M

F

F

N

N

I

I

N

R

T

T

L

M

Q

D

W

W

D

D

E

D

K

D

L

L

L

L

K

K

A

L

L

F

D

N

I

I

P

P

L

R

S

A

M

I

M

L

P

P

E

E

V

V

K

K

P

Q

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

E

T

T

N

S

I

-

G

-

G

-

K

T

G

T

G

S

T

T

R

K

I

I

P

P

I

I

S

A

G

G

I

I

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

C

V

T

E

K

Q

P

G

G

Q

M

A

V

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F

F

L

L

L

L

M

M

N

N

T

T

G

G

K

N

D

E

K

A

V

V

T

Y

S

S

H

K

N

N

G

N

L

L

L

L

T

T

T

T

L

V

A

A

C

W

G

K

P

I

K

N

G

G

D

E

V

V

A

S

Y

Y

A

A

L

L

E

E

G

G

A

S

V

V

F

F

M

V

G

G

G
|
G

A

A

S

A

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

G

V

L

K

K

L

I

L

I

S

H

D

D

A

S

S
|
S

D

E

S

V

E

S

Y

T

F

L

A

A

E

E

K

T

V

V

G

E

S

D

A

N

N

G

G

G

V

V

Y

Y

V

F

V

V

P

P

A

A

F

L

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

Q

Y

Y

A

A

R

R

G

G

A

T

I

I

V

I

G

G

L

V

T

T

R

R

G

G

V

T

N

T

S

D

N

G

H

H

I

I

I

A

R

R

A

A

T

T

L

L

E

E

S

G

V

I

A

A

Y

F

Q

Q

T

V

R

Y

D

D

V

I

L

V

D

K

A

A

M

M

Q

E

A

A

D

D

S

A

G

E

I

T

K

Q

L

S

A

T

N

E

L

L

R

R

V

V

D

D

G
|
G

G
|
G

A
|
A

V

S

A

A

N

S

N

N

F

L

L

L

M

M

Q

Q

F

I

Q

Q

S

S

D

D

I

L

L

F

N

G

T

F

E

K

V

I

H

I

R

R

P

P

V

K

V

T

T

L

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

E

S

S

I

I

D

D

E

E

L

I

K

Q

D

S

K

Q

A

W

V

I

L

I

D

E

R

K

S

E

F

F

E

T

P

P

C

K

A

E

N

D

D

K

K

T

K

K

R

I

D

D

R

N

R

M

Y

V

R

S

G

F

W

W

Q

H

R

K

A

A

V

V

E

K

C

R

A

S

K

Q

G

A

W

W

A

I

V

E

D

D

binding adenosine-5'-diphosphate: R16 (= R19), G262 (= G268), T263 (= T269), G306 (= G312), I309 (= I315), S323 (= S329), G406 (= G412), G407 (= G413), A408 (= A414)
binding magnesium ion: G11 (= G14), T12 (= T15), T13 (= T16), S14 (= S17)

O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
56% identity, 97% coverage: 4:496/506 of query aligns to 3:495/506 of O34153

query
sites
O34153

E

E

Q

K

K

N

Y

Y

I

V

V

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

V

I

V

I

L

F

D

D

H

R

N

N

A

G

N

K

I

K

V

I

C

G

T

S

A

S

Q

Q

R

K

E

E

F

F

T

P

Q

Q

I

Y

Y

F

P

P

K

K

A

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

L

N

E

E

I

I

Y

W

A

N

T

S

Q

V

S

Q

S

S

T

V

L

I

V

A

E

G

A

A

L

F

A

I

K

E

A

S

G

G

I

I

R

R

S

P

D

E

Q

A

V

I

A

A

G

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

K

K

N

T

T

T

G

G

K

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

S

A

S

D

P

I

I

C

A

E

D

E

Q

L

L

K

K

K

V

Q

D

D

G

L

H

E

T

E

E

Y

M

I

I

R

H

D

E

N

K

T

T

G

G

L

L

V

V

V

I

D

D

P

A

Y
|
Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

E

E

G

G

A

A

R

Q

E

E

E

K

A

A

E

D

A

N

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

I

V

W

W

K

K

M

L

T

T

Q

D

G

G

R

Q

V

V

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

Y

N

N

I

I

N

H

T

K

L

L

Q

E

W

W

D

D

E

Q

K

E

L

I

L

L

K

D

A

L

L

L

D

N

I

I

P

P

L

S

S

S

M

M

M

L

P

P

E

E

V

V

K

K

P

S

S

N

S

S

E

E

V

V

Y

Y

G

G

Q

H

T

T

N

R

I

S

G

Y

G
x
H

K

F

G

Y

G

G

T

S

R

E

I

V

P

P

I

I

S

A

G

G

I

M

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

V

F

E

E

Q

K

G

G

Q

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

A

F

F

L

I

L

V

M

M

N

N

T

T

G

G

K

E

D

E

K

P

V

Q

T

L

S

S

H

D

N

N

G

D

L

L

L

L

T

T

T

T

L

I

A

G

C

Y

G

G

P

I

K

N

G

G

D

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

A

S

V

I

F

F

M

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

V

L

K

R

L

M

L

I

S

E

D

T

A

S

S

P

D

Q

S

S

E

E

Y

E

F

L

A

A

E

A

K

K

V

A

G

K

S

G

A

D

N

N

G

E

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

Y

E

A

A

R

R

G

G

A

A

I

V

V

F

G

G

L

L

T

T

R

R

G

G

V

T

N

T

S

K

N

E

H

D

I

F

I

V

R

R

A

A

T

T

L

L

E

Q

S

A

V

V

A

A

Y

Y

Q

Q

T

S

R

K

D

D

V

V

L

I

D

D

A

T

M

M

Q

K

A

K

D

D

S

S

G

G

I

I

K

D

L

I

A

P

N

L

L

L

R

K

V

V

D

D

G

G

G

G

A

A

V

A

A

K

N

N

N

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

D

T

I

E

D

V

V

H

Q

R

R

P

A

V

A

V

N

T

L

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

K

S

D

I

L

D

D

E

E

L

L

K

K

D

S

K

M

A

A

V

E

L

E

D

G

R

Q

S

M

F

F

E

T

P

P

C

E

A

M

N

P

D

A

K

E

K

E

R

R

D

D

R

N

R

L

Y

Y

R

E

G

G

W

W

Q

K

R

Q

A

A

V

V

E

A

C

A

A

T

K

Q

R84 (= R85) binding glycerol
E85 (= E86) binding glycerol
Y136 (= Y137) binding glycerol
H232 (≠ G233) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
D246 (= D247) binding glycerol
Q247 (= Q248) binding glycerol

3h3nX Glycerol kinase h232r with glycerol (see paper)
56% identity, 97% coverage: 4:496/506 of query aligns to 2:494/501 of 3h3nX

query
sites
3h3nX

E

E

Q

K

K

N

Y

Y

I

V

V

M

A

A

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

A

A

V

I

V

I

L

F

D

D

H

R

N

N

A

G

N

K

I

K

V

I

C

G

T

S

A

S

Q

Q

R

K

E

E

F

F

T

P

Q

Q

I

Y

Y

F

P

P

K

K

A

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

L

N

E

E

I

I

Y

W

A

N

T

S

Q

V

S

Q

S

S

T

V

L

I

V

A

E

G

A

A

L

F

A

I

K

E

A

S

G

G

I

I

R

R

S

P

D

E

Q

A

V

I

A

A

G

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

K

K

N

T

T

T

G

G

K

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

S

R

R

R

Q

T

S

A

S

D

P

I

I

C

A

E

D

E

Q

L

L

K

K

K

V

Q

D

D

G

L

H

E

T

E

E

Y

M

I

I

R

H

D

E

N

K

T

T

G

G

L

L

V

V

V

I

D

D

P

A

Y
|
Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

E

E

G

G

A

A

R

Q

E

E

E

K

A

A

E

D

A

N

G

G

N

E

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

I

V

W

W

K

K

M

L

T

T

Q

D

G

G

R

Q

V

V

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

Y

N

N

I

I

N

H

T

K

L

L

Q

E

W

W

D

D

E

Q

K

E

L

I

L

L

K

D

A

L

L

L

D

N

I

I

P

P

L

S

S

S

M

M

M

L

P

P

E

E

V

V

K

K

P

S

S

N

S

S

E

E

V

V

Y

Y

G

G

Q

H

T

T

N

R

I

S

G

Y

G

R

K

F

G

Y

G

G

T

S

R

E

I

V

P

P

I

I

S

A

G

G

I

M

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

V

F

E

E

Q

K

G

G

Q

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

A

F
|
F

L

I

L

V

M

M

N

N

T

T

G

G

K

E

D

E

K

P

V

Q

T

L

S

S

H

D

N

N

G

D

L

L

L

L

T

T

T

T

L

I

A

G

C

Y

G

G

P

I

K

N

G

G

D

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

A

S

V

I

F

F

M

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

V

L

K

R

L

M

L

I

S

E

D

T

A

S

S

P

D

Q

S

S

E

E

Y

E

F

L

A

A

E

A

K

K

V

A

G

K

S

G

A

D

N

N

G

E

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

Y

E

A

A

R

R

G

G

A

A

I

V

V

F

G

G

L

L

T

T

R

R

G

G

V

T

N

T

S

K

N

E

H

D

I

F

I

V

R

R

A

A

T

T

L

L

E

Q

S

A

V

V

A

A

Y

Y

Q

Q

T

S

R

K

D

D

V

V

L

I

D

D

A

T

M

M

Q

K

A

K

D

D

S

S

G

G

I

I

K

D

L

I

A

P

N

L

L

L

R

K

V

V

D

D

G

G

G

G

A

A

V

A

A

K

N

N

N

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

D

T

I

E

D

V

V

H

Q

R

R

P

A

V

A

V

N

T

L

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

K

S

D

I

L

D

D

E

E

L

L

K

K

D

S

K

M

A

A

V

E

L

E

D

G

R

Q

S

M

F

F

E

T

P

P

C

E

A

M

N

P

D

A

K

E

K

E

R

R

D

D

R

N

R

L

Y

Y

R

E

G

G

W

W

Q

K

R

Q

A

A

V

V

E

A

C

A

A

T

K

Q

binding glycerol: R83 (= R85), E84 (= E86), W103 (= W105), Y135 (= Y137), D245 (= D247), F270 (= F272)
binding phosphate ion: T13 (= T15), T267 (= T269)

O34154 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus faecalis (strain ATCC 700802 / V583) (see 2 papers)
56% identity, 97% coverage: 4:496/506 of query aligns to 3:494/501 of O34154

query
sites
O34154

E

E

Q

E

K

K

Y

Y

I

I

V

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

V

I

V

I

L

F

D

D

H

K

N

K

A

G

N

N

I

K

V

I

C

G

T

S

A

S

Q

Q

R

K

E

E

F

F

T

T

Q

Q

I

Y

Y

F

P

P

K

N

A

A

G

G

W

W

V

V

E

E

H

H

D

N

P

A

L

N

E

E

I

I

Y

W

A

N

T

S

Q

V

S

Q

S

S

T

V

L

I

V

A

E

G

A

S

L

L

A

I

K

E

A

S

G

G

I

V

R

K

S

P

D

T

Q

D

V

I

A

A

G

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

V

V

W

W

D

D

K

K

N

A

T

T

G

G

K

L

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

T

A

T

D

P

I

I

C

A

E

D

E

Q

L

L

K

K

K

E

Q

D

D

G

L

Y

E

S

E

E

Y

M

I

I

R

H

D

E

N

K

T

T

G

G

L

L

V

I

V

I

D

D

P

A

Y

Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

I

L

L

D

D

N

H

V

V

E

E

G

G

A

A

R

Q

E

E

E

R

A

A

E

E

A

N

G

G

N

E

L

L

L

M

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

I

V

W

W

K

K

M

L

T

T

Q

-

G

G

R

D

V

T

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

H

T

D

L

L

Q

D

W

W

D

D

E

Q

K

E

L

I

L

L

K

D

A

L

L

L

D

N

I

I

P

P

L

R

S

V

M

M

M

L

P

P

E

K

V

V

K

V

P

S

S

N

S

S

E

E

V

V

Y

Y

G

G

Q

L

T

T

N

K

I

N

G

Y

G
x
H

K

F

G

Y

G

G

T

S

R

E

I

V

P

P

I

I

S

A

G

G

I

M

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

V

F

E

E

Q

P

G

G

Q

M

A

V

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

S

F

F

L

I

L

V

M

M

N

N

T

T

G

G

K

E

D

E

K

P

V

Q

T

L

S

S

H

K

N

N

G

N

L

L

L

L

T

T

T

T

L

I

A

G

C

Y

G

G

P

I

K

N

G

G

D

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

A

S

V

I

F

F

M

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

V

L

K

K

L

M

L

L

S

Q

D

T

A

A

S

A

D

E

S

S

E

E

Y

A

F

V

A

A

E

K

K

A

V

S

G

T

S

G

A

H

N

N

G

E

V

V

Y

Y

V

V

V

V

P

P

A

A

F

F

T

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

Y

Q

A

A

R

R

G

G

A

A

I

V

V

F

G

G

L

L

T

T

R

R

G

G

V

T

N

T

S

R

N

E

H

D

I

F

I

V

R

K

A

A

T

T

L

L

E

Q

S

A

V

V

A

A

Y

Y

Q

Q

T

V

R

R

D

D

V

I

L

I

D

D

A

T

M

M

Q

K

A

E

D

D

S

T

G

G

I

I

K

D

L

I

A

P

N

V

L

L

R

K

V

V

D

D

G

G

G

G

A

A

V

A

A

N

N

N

N

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

N

T

T

E

A

V

V

H

Q

R

R

P

A

V

H

V

N

T

L

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

T

K

S

D

I

L

D

E

E

E

L

I

K

K

D

A

K

F

A

Q

V

E

L

E

D

G

R

Q

S

Q

F

F

E

E

P

P

C

I

A

M

N

A

D

E

K

E

K

E

R

R

D

E

R

D

R

L

Y

Y

R

E

G

G

W

W

Q

Q

R

Q

A

A

V

V

E

A

C

A

A

T

K

Q

H231 (≠ G233) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6k76A Glycerol kinase form thermococcus kodakarensis, complex structure with substrate.
56% identity, 98% coverage: 8:503/506 of query aligns to 1:485/485 of 6k76A

query
sites
6k76A

I

V

V

L

A

S

L

L

D

D

Q

E

G

G

T
|
T

T

T

S

S

S

A

R

R

A

A

V

I

V

I

L

F

D

D

H

R

N

E

A

S

N

N

I

I

V

H

C

G

T

I

A

G

Q

Q

R

Y

E

E

F

F

T

P

Q

Q

I

H

Y

Y

P

P

K

R

A

P

G

G

W

W

V

V

E

E

H

H

D

N

P

P

L

E

E

E

I

I

Y

W

A

D

T

A

Q

Q

S

L

S

R

T

A

L

I

V

K

E

D

A

A

L

I

A

Q

K

S

A

A

G

R

I

-

R

E

S

P

D

N

Q

Q

V

I

A

A

G

A

I

I

G

G

I

V

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

L

V

V

W

W

D

D

K

K

N

D

T

-

G

G

K

K

P

P

V

L

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

I

M

C

V

E

E

E

E

L

I

K

K

K

R

Q

E

D

-

L

Y

E

G

E

T

Y

M

I

I

R

K

D

E

N

K

T

T

G

G

L

L

V

V

V

P

D

D

P

A

Y

Y

F

F

S

S

G

A

T

S

K

K

I

L

K

K

W

W

I

L

L

L

D

D

N

N

V

V

E

P

G

G

A

L

R

R

E

E

E

K

A

A

E

E

A

K

G

G

N

E

L

V

L

M

F

F

G

G

T

T

V

V

D

D

T

T

W

F

L

L

I

I

W

Y

K

R

M

L

T

T

Q

G

G

E

R

-

V

-

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

V

L

F

F

N

N

I

I

N

K

T

K

L

L

Q

D

W

W

D

D

E

D

K

E

L

L

L

L

K

E

A

L

L

F

D

D

I

I

P

P

L

E

S

S

M

V

M

L

P

P

E

E

V

V

K

R

P

E

S

S

S

S

E

E

V

V

Y

Y

G

G

Q

Y

T

T

N

K

I

-

G

-

G

-

K

K

G

E

G

L

T

A

R

E

I

I

P

P

I

V

S

S

G

G

I

D

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

A

C

A

V

F

E

E

Q

A

G

G

Q

M

A

V

K

K

N

A

T

T

Y

Y

G

G

T
|
T

G

G

C

S

F

F

L

I

L

L

M

V

N

N

T

T

G

D

K

K

D

M

K

V

V

L

T

Y

S

S

H

D

N

N

G

-

L

L

L

L

T

T

T

T

L

I

A

A

C

W

G

G

P

L

K

N

G

G

D

R

V

V

A

S

Y

Y

A

A

L

L

E

E

G

G

A

S

V

I

F

F

M

V

G

T

G
|
G

A

A

S

A

I
x
V

Q
|
Q

W

W

L

L

R

R

D

D

E

G

V

I

K

K

L

I

L

I

S

K

D

H

A

A

S

S

D

E

S

T

E

E

Y

E

F

L

A

A

E

T

K

K

V

L

G

E

S

S

A

N

N

E

G

G

V

V

Y

Y

V

F

V

V

P

P

A

A

F

F

T

V

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

Q

Y

F

A

A

R

R

G

G

A

I

I

I

V

I

G

G

L

I

T

T

R

R

G

G

V

T

N

G

S

R

N

E

H

H

I

L

I

A

R

R

A

A

T

T

L

L

E

E

S

A

V

I

A

A

Y

Y

Q

L

T

T

R

R

D

D

V

V

L

V

D

D

A

E

M

M

Q

E

A

-

D

-

S

K

G

L

I

V

K

Q

L

I

A

K

N

E

L

L

R

R

V

V

D

D

G

G

G
|
G

A
|
A

V

T

A

A

N
|
N

N

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

N

T

R

E

K

V

V

H

I

R

R

P

P

V

V

V

V

T

K

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

D

F

Y

W

W

T

A

S

D

I

T

D

R

E

E

L

I

K

A

D

E

K

L

A

W

V

K

L

A

D

E

R

R

S

I

F

F

E

E

P

P

C

K

A

M

N

D

D

E

K

K

K

T

R

R

D

E

R

R

R

L

Y

Y

R

K

G

G

W

W

Q

K

R

E

A

A

V

V

E

K

C

R

A

A

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M

G

G

W

W

A

A

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K

D

V

I

V

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D

binding phosphoaminophosphonic acid-adenylate ester: T8 (= T15), T254 (= T269), G296 (= G312), V299 (≠ I315), Q300 (= Q316), G395 (= G413), A396 (= A414), N399 (= N417)

O86033 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
52% identity, 96% coverage: 7:493/506 of query aligns to 4:491/497 of O86033

query
sites
O86033

Y

Y

I

I

V

L

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

T

R

R

A

A

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I

V

V

L

F

D

D

H

G

N

N

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Q

N

K

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I

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A

C

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A

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Q

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E

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F

F

T

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Y

F

P

P

K

K

A

S

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G

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W

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V

E

E

H

H

D

D

P

P

L

E

E

E

I

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A

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Q

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L

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V

K

E

E

A

A

L

I

A

E

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K

A

S

G

G

I

I

R

T

S

A

D

N

Q

D

V

I

A

A

G

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

V

I

V

V

V

W

W

D

D

K

R

N

E

T

T

G

G

K

K

P

P

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Y

H

N

N

A

A

I

I

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V

W

W

Q

Q

C

D

R

R

R

R

T

T

A

A

D

A

I

F

C

C

E

D

E

K

L

L

K

K

K

K

Q

K

D

G

L

L

E

E

E

K

Y

T

I

F

R

V

D

K

N

K

T

T

G

G

L

L

V

L

V

L

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

L

K

N

W

W

I

L

L

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D

S

N

N

V

V

E

K

G

G

A

A

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E

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E

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A

A

E

A

A

K

G

G

N

E

L

L

L

C

F

F

G

G

T

T

V

I

D

D

T

T

W

F

L

L

I

I

W

W

K

R

M

L

T

T

Q

G

G

G

R

E

V

C

H

F

V

C

T

T

D

D

Y

A

T

T

N

N

A

A

S

S

R

R

T

T

M

L

V

L

F

Y

N

N

I

I

N

A

T

E

L

N

Q

A

W

W

D

D

E

D

K

E

L

L

L

T

K

E

A

V

L

L

D

R

I

V

P

P

L

K

S

E

M

M

M

L

P

P

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E

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V

K

K

P

D

S

C

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A

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A

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Y

F

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Q

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T

N

D

I

P

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G

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G

G

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-

I

I

P

P

I

I

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L

G

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A

A

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D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

T

F

I

G

G

Q

Q

M

A

C

C

V

F

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K

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P

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G

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A

L

K

K

N

S

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

A

L

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L

N

N

T

T

G

G

K

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D

D

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M

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N

N

G

R

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L

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I

A

A

C

Y

G

R

P

L

K

D

G

G

D

E

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A

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A

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A

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A

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F

E

E

P

P

C

H

A

M

N

D

D

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K

A

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K

R82 (= R85) binding glycerol
E83 (= E86) binding glycerol
Y134 (= Y137) binding glycerol
D243 (= D247) binding glycerol
Q244 (= Q248) binding glycerol

8y1tA Crystal structure of trypanosoma brucei gambiense glycerol kinase complex with adp and g3p
42% identity, 98% coverage: 6:499/506 of query aligns to 5:513/514 of 8y1tA

query
sites
8y1tA

K

K

Y

Y

I

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A

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R

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E

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I

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A

W

W

D

D

K

R

N

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E

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C

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A

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W

Q

N

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D

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L

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R

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A

Y

D

D

I

I

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E

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-

K

-

V

-

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-

A

E

E

L

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K

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Q

G

D

D

L

-

E

S

E

M

Y

F

I

A

R

S

D

K

N

I

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T

G

G

L

L

V

P

V

V

D

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P

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Y

Y

F

F

S

A

G

A

T

F

K

K

I

M

K

R

W

W

I

M

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L

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E

N

N

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V

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P

G

A

A

V

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A

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E

A

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A

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N

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L

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W

W

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L

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W

Y

K

K

M

L

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Q

G

G

G

R

K

V

A

H

F

V

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T

T

D

D

Y

V

T

T

N

N

A

A

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R
|
R

T

T

M

F

V

L

F

M

N

D

I

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N

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T

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W

W

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E

L

L

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E

A

K

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K

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M

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M

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R

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N

S

S

E

E

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L

Y

F

G

G

-

Y

-

V

-

E

-

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-

D

Q

E

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N

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G

A

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K

A

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G

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G

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A

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|
D

Q
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Q

Q

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A

S

A

A

L

L

F

F

G

G

Q

N

M

M

C

C

V

F

E

E

Q

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G

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A

A

K

K

N

N

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Y

Y

G
|
G

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|
T

G

G

C

C

F

F

L

L

L

L

M

M

N

N

T

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E

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E

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N

H

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F

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D

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A

Y

Y

Y

A

A

L

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A

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A

L

L

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A

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A

A

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Q

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N

D

D

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V

D

G

A

S

M

M

Q

K

A

R

D

D

S

A

G

G

I

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N

L

L

A

S

N

S

L

L

R

R

V

V

D

D

G

G

G
|
G

A
x
L

V

S

A

K

N

N

N

G

F

L

L

L

M

M

Q

E

F

I

Q

Q

S

A

D

S

I

L

L

L

N

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E

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L

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M

T

H

E

E

V

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

L

L

C

A

A

G

G

L

L

A

A

V

A

G

G

F

V

W

W

T

T

S

S

I

L

D

E

E

E

L

V

K

K

D

A

K

V

A

S

V

R

L

R

D

E

R

N

S

S

F

W

E

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-

T

-

V

-

S

P

P

C

S

A

G

N

S

D

A

K

M

K

E

R

R

D

E

R

A

R

M

Y

I

R

A

G

E

W

W

Q

R

R

E

A

A

V

L

E

K

C

R

A

T

K

K

G

-

W

W

A

A

binding adenosine-5'-diphosphate: G278 (= G268), T279 (= T269), G425 (= G413), L426 (≠ A414)
binding sn-glycerol-3-phosphate: R87 (= R85), E88 (= E86), W107 (= W105), R195 (= R190), D257 (= D247), Q258 (= Q248)

Query Sequence

>WP_068330536.1 NCBI__GCF_001650345.1:WP_068330536.1
MTTEQKYIVALDQGTTSSRAVVLDHNANIVCTAQREFTQIYPKAGWVEHDPLEIYATQSS
TLVEALAKAGIRSDQVAGIGITNQRETTIVWDKNTGKPVYNAIVWQCRRTADICEELKKQ
DLEEYIRDNTGLVVDPYFSGTKIKWILDNVEGAREEAEAGNLLFGTVDTWLIWKMTQGRV
HVTDYTNASRTMVFNINTLQWDEKLLKALDIPLSMMPEVKPSSEVYGQTNIGGKGGTRIP
ISGIAGDQQAALFGQMCVEQGQAKNTYGTGCFLLMNTGKDKVTSHNGLLTTLACGPKGDV
AYALEGAVFMGGASIQWLRDEVKLLSDASDSEYFAEKVGSANGVYVVPAFTGLGAPYWDP
YARGAIVGLTRGVNSNHIIRATLESVAYQTRDVLDAMQADSGIKLANLRVDGGAVANNFL
MQFQSDILNTEVHRPVVTEVTALGAAYLAGLAVGFWTSIDELKDKAVLDRSFEPCANDKK
RDRRYRGWQRAVECAKGWAVDIDDEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory