SitesBLAST

Comparing WP_068330987.1 NCBI__GCF_001650345.1:WP_068330987.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
35% identity, 97% coverage: 8:418/424 of query aligns to 13:427/427 of 5e9sA

• binding aspartic acid: R274 (≠ A271), S275 (= S272), S276 (= S273), T313 (≠ V310), G353 (= G350), V354 (= V351), A357 (≠ G354), G358 (= G355), D394 (= D384), R397 (≠ C387), T398 (= T388)
• binding decyl-beta-d-maltopyranoside: L194 (≠ K190), G198 (≠ L194), Y202 (≠ L198)
• binding sodium ion: Y87 (= Y88), T90 (≠ N91), S91 (≠ T92), S276 (= S273), G305 (= G302), A306 (= A303), T307 (= T304), N309 (= N306), N309 (= N306), M310 (= M307), D311 (≠ S308), S348 (= S345), I349 (= I346), G350 (= G347), T351 (≠ A348), N401 (= N391), V402 (= V392), D405 (= D395)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
35% identity, 97% coverage: 8:418/424 of query aligns to 11:425/425 of 6zgbA

• binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A271), S273 (= S272), S274 (= S273), M308 (= M307), D392 (= D384), R395 (≠ C387), T396 (= T388)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
35% identity, 97% coverage: 8:417/424 of query aligns to 13:426/426 of 6xwnB

• binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S272), S276 (= S273), M310 (= M307), T313 (≠ V310), D394 (= D384), R397 (≠ C387)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
35% identity, 97% coverage: 8:418/424 of query aligns to 10:424/424 of 6zl4A

• binding decyl-beta-d-maltopyranoside: L191 (≠ K190), G195 (≠ L194), R282 (= R282)
• binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A271), S272 (= S272), S273 (= S273), M307 (= M307), T310 (≠ V310), G353 (= G353), A354 (≠ G354), R394 (≠ C387), T395 (= T388)

Sites not aligning to the query:

• binding decyl-beta-d-maltopyranoside: 3

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
35% identity, 97% coverage: 8:418/424 of query aligns to 6:416/416 of 6r7rA

• binding d-aspartic acid: R263 (≠ A271), S265 (= S273), M299 (= M307), T302 (≠ V310), T340 (≠ A348), G342 (= G350), V343 (= V351), G347 (= G355), D383 (= D384), R386 (≠ C387), T387 (= T388), N390 (= N391)
• binding decyl-beta-d-maltopyranoside: H23 (≠ L26), V212 (≠ L219), A216 (= A223)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
34% identity, 94% coverage: 8:406/424 of query aligns to 7:408/408 of 6bauA

• binding cysteine: S270 (= S273), M303 (= M307), T306 (≠ V310), A345 (≠ G349), G346 (= G350), V347 (= V351), G351 (= G355), D386 (= D384), C389 (= C387), T390 (= T388), N393 (= N391)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
34% identity, 94% coverage: 8:406/424 of query aligns to 7:408/409 of 6bavA

• binding benzylcysteine: R268 (≠ A271), S270 (= S273), T306 (≠ V310), Y309 (= Y313), D382 (= D380), D386 (= D384), C389 (= C387), T390 (= T388)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
34% identity, 94% coverage: 8:406/424 of query aligns to 6:407/407 of 2nwwA

• binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S273), T305 (≠ V310), A349 (≠ G354), G350 (= G355), D385 (= D384), R388 (≠ C387), T389 (= T388), N392 (= N391)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
33% identity, 95% coverage: 8:410/424 of query aligns to 15:420/425 of O59010

• S65 (= S63) mutation to V: Strongly decreased chloride conductance.
• R276 (≠ A271) mutation to S: Increased rate of aspartate transport; when associated with R-395.
• RSS 276:278 (≠ ASS 271:273) binding L-aspartate
• M311 (= M307) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
• T314 (≠ V310) binding L-aspartate
• V355 (= V351) binding L-aspartate
• D394 (= D384) binding L-aspartate
• M395 (= M385) mutation to R: Increased rate of aspartate transport; when associated with S-276.
• R397 (≠ C387) mutation to A: Strongly decreased affinity for aspartate.
• N401 (= N391) binding L-aspartate
• D405 (= D395) mutation to N: Strongly decreased affinity for aspartate.

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
34% identity, 94% coverage: 8:406/424 of query aligns to 12:413/413 of 6x14A

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G67), V83 (≠ F85), I157 (≠ L162), Y164 (≠ A169), K193 (= K190), T305 (= T304), I306 (≠ M305), I347 (= I346)
• binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I9), M199 (= M196), S275 (= S273), T311 (≠ V310), G356 (= G355), L384 (≠ A377), D391 (= D384), R394 (≠ C387)

Sites not aligning to the query:

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
34% identity, 94% coverage: 8:406/424 of query aligns to 15:416/419 of 6x15A