SitesBLAST

C9 (≠ F9) mutation to S: No effect.
C20 (= C20) mutation to S: Loss of oligomerization; no alteration of water permeability.
T183 (= T182) mutation to C: No effect.
R189 (= R188) mutation R->V,S: Loss of function.

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
64% identity, 99% coverage: 1:229/231 of query aligns to 3:232/232 of 2o9eA

query
sites
2o9eA

M

M

F

F

K

R

K

K

L

L

V

A

A

A

E

E

F

S

I

F

G

G

T

T

F

F

W

W

L

L

V

V

L

F

G

G

C

S

G

G

S

S

A

A

V

V

L

L

A

A

A

G

F

F

P

P

E

E

V

L

G

G

I

I

G

G

L

F

L

A

G

G

V

V

S

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

V

L

T

T

M

M

A

A

Y

F

A

A

I

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

L

F

N

N

P

P

A

A

V

V

T

T

V

I

G

G

L

L

W

W

S

A

G

G

R

R

F

F

P

P

A

A

A

K

D

E

V

V

P

G

Y

Y

I

V

L

I

A

A

Q

Q

V

V

L

V

G

G

A

G

I

I

V

V

G

A

A

A

F

A

V

L

L

L

Y

Y

I

L

I

I

A

A

S

S

G

G

Q

K

A

T

G

G

F

F

E

D

L

A

A

A

G

A

-

S

G

G

L

F

A

A

S

S

N

N

G

G

Y

Y

G

G

E

E

H

H

S

S

P

P

G

G

Y

Y

T

S

L

M

M

L

S

S

G

A

F

L

V

V

T

V

E
|
E

F

L

V

V

M

L

T

S

F

A

M

G

F

F

L

L

I

L

V

V

I

I

L

H

G

G

A

A

T

T

H

D

K

K

L

F

A

A

T

P

P

A

A

G

M

F

A

A

G

P

L

I

A

A

I

I

G

G

L

L

A

A

L

L

T

T

L

L

I

I

H

H

L

L

I

I

S
|
S

I

I

P

P

V

V

T

T

N

N

T

C

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

P

V

A

A

L

I

M

F

V

Q

G

G

D

G

W

W

A

A

V

L

A

E

Q

Q

L

L

W

W

L

F

F

F

W

W

L

V

A

V

P

P

L

I

L

V

G

G

A

G

L

I

V

I

A

G

G

G

I

L

V

I

Y

Y

R

R

W

T

L

L

S

L

P

E

Q

K

D

R

binding mercury (ii) ion: E140 (= E137), S179 (= S176)

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
65% identity, 97% coverage: 1:225/231 of query aligns to 3:228/230 of 3nkaA

query
sites
3nkaA

M

M

F

F

K

R

K

K

L

L

V

A

A

A

E

E

F

C

I

F

G

G

T

T

F

F

W

W

L

L

V

V

L

F

G

G

C

C

G

G

S

S

A
|
A

V

V

L

L

A

A

A

G

F

F

P

P

E

E

V

L

G

G

I

I

G

G

L

F

L

A

G

G

V
|
V

S

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

V

L

T

T

M

M

A

A

Y

F

A

A

I

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

L

F

N

N

P

P

A

A

V

V

T

T

V

I

G

G

L

L

W

W

S

A

G

G

R

R

F

F

P

P

A

A

A

K

D

E

V

V

P

G

Y

Y

I

V

L

I

A

A

Q

Q

V

V

L

V

G

G

A

G

I

I

V

V

G

A

A

A

F

A

V

L

L

L

Y

Y

I

L

I

I

A

A

S

S

G

G

Q

K

A

T

G

G

F

F

E

D

L

A

A

A

G

A

-

S

G

G

L

F

A

A

S

S

N

N

G

G

Y

Y

G

G

E

E

H

H

S

S

P

P

G

G

Y

Y

T

S

L

M

M

L

S

S

G

A

F

L

V

V

T

V

E

E

F

L

V

V

M

L

T

S

F

A

M

G

F

F

L

L

I

L

V

V

I

I

L

H

G

G

A

A

T

T

H

D

K

K

L

F

A

A

T

P

P

A

A

G

M

F

A

A

G

P

L

I

A

A

I

I

G

G

L

L

A

A

L

L

T

T

L

L

I

I

H

G

L

L

I

I

S

S

I
|
I

P

P

V

V

T

T

N
|
N

T

F

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

P

V

A

A

L

I

M

F

V

Q

G

G

D

G

W

W

A

A

V

L

A

E

Q

Q

L

L

W

W

L

F

F

F

W

W

L

V

A

V

P

P

L

I

L

V

G

G

A

G

L

I

V

I

A

G

G

G

I

L

V

I

Y

Y

R

R

W

T

L

L

binding glycerol: A25 (= A23), V41 (= V39), I180 (= I177), N184 (= N181)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
38% identity, 99% coverage: 3:230/231 of query aligns to 38:252/271 of P08995

query
sites
P08995

K

Q

K

K

L

L

V

V

A

A

E

E

F

A

I

V

G

G

T

T

F

Y

W

F

L

L

V

I

L

F

G

A

G

G

C

C

G

A

S

S

A

L

V

V

L

V

A

N

A

E

A

N

F

Y

P

Y

E

N

V

M

G

-

I

I

G

T

L

F

L

P

G

G

V

I

S

A

L

I

A

V

F

W

G

G

L

L

T

V

V

L

V

T

T

V

M

L

A

V

Y

Y

A

T

I

V

G

G

H

H

I

I

S

S

G

G

C

G

H

H

L

F

N

N

P

P

A

A

V

V

T

T

V

I

G

A

L

F

W

A

S

S

G

T

G

R

R

R

F

F

P

P

A

L

A

I

D

Q

V

V

V

P

P

A

Y

Y

I

V

L

V

A

A

Q

Q

V

L

L

L

G

G

A

S

I

I

V

L

G

A

A

S

F

G

V

T

L

L

Y

R

I

L

A

F

S

M

G

G

Q

N

A

-

G

-

F

H

E

D

L

Q

A

F

G

S

G

G

L

T

A

V

S

P

N

N

G

G

Y

-

G

-

E

-

H

-

S

-

P

-

G

-

Y

-

T

T

L

N

M

L

S

Q

G

A

F

F

V

V

T

F

E

E

F

F

V

I

M

M

T

T

F

F

M

F

F

L

L

M

I

F

V

V

I

I

L

C

G

G

-

V

A

A

T

T

H

D

K

N

L

R

A

A

T

V

P

G

A

E

M

F

A

A

G

G

L

I

A

A

I

I

G

G

L

S

A

T

L

L

T

L

L

L

I

N

H

V

L

I

I

I

S

G

I

G

P

P

V

V

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

P

A

A

L

F

M

V

V

H

G

G

D

E

W

Y

A

-

V

-

A

E

Q

G

L

I

W

W

L

I

F

Y

W

L

L

L

A

A

P

P

L

V

G

G

A

A

L

I

V

A

A

G

G

A

I

W

V

V

Y

Y

R

N

W

I

L

V

S

R

P

Y

Q

T

D

D

D

K

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
36% identity, 99% coverage: 2:229/231 of query aligns to 35:255/323 of P55088

query
sites
P55088

F

W

K

K

K

A

L

V

V

S

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

I

L

F

V

V

L

L

G

L

G

G

C

V

G

G

S

S

A

T

V

I

L

N

-

W

A

G

A

S

F

E

P

N

E

P

V

L

G

P

I

V

G

D

L

M

L

V

G

L

V

I

S

S

L

L

A

C

F

F

G

G

L

L

T

S

V

I

V

A

T

T

M

M

A

V

Y

Q

A

C

I

F

G

G

H

H

I

I

S

S

G

G

C

G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

V

G

A

L

M

W

V

S

C

G

T

G

R

R

K

F

I

P
x
S

A

I

A

A

D

K

V

S

V

V

P

F

Y

Y

I

I

L

I

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

V

I

G

G

A

A

F

G

V

I

L

L

Y

Y

I

L

I

V

A

T

S

P

G

P

Q

S

A

-

G

-

F

-

E

-

L

V

A

V

G

G

L

L

A

G

S

V

N

T

G

T

Y

V

G

-

E

-

H

-

S

-

P

H

G

G

G

N

Y

L

T

T

L

A

M

G

S

H

G

G

F

L

V

L

T

V

E

E

F

L

V

I

M

I

T

T

F

F

M

Q

F

L

L

V

I

F

V

T

I

I

L

F

G

A

A

S

T

C

H

D

K

S

L

K

A

R

T

T

P

D

A

V

M

T

A

G

G

S

-

I

-

A

L

L

A

A

I

I

G

G

L

F

A

S

L

V

T

A

L

I

I

G

H

H

L

L

I

F

S

A

I

I

P

N

V

Y

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

M

I

V

M

G

G

D

N

W

W

A

-

V

-

A

A

Q

N

L

H

W

W

L

I

F

Y

W

W

L

V

A

G

P

P

L

I

L

M

G

G

A

A

L

V

V

L

A

A

G

G

I

A

V

L

Y

Y

R

E

W

Y

L

V

S

F

P

C

Q

P

D

D

S111 (≠ P77) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
36% identity, 97% coverage: 3:226/231 of query aligns to 6:244/245 of 2evuA

query
sites
2evuA

K

K

K

R

L

C

V

I

A

A

E

E

F

F

I

I

G

G

T

T

F

F

W

I

L

L

V

V

L

F

G

F

G

G

C

A

G

G

S

S

A

A

V

A

L

V

A

T

A

L

A

M

F

I

P

A

E

S

V

G

G

G

I

T

G

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

-

G

-

D

L
x
W

L

V

G

A

V

I

S

G

L

L

A

A

F

F

G

G

L

F

T

A

V
x
I

V

A

T

A

M

S

A

I

Y

Y

A

A

I

L

G

G

H

N

I

I

S

S

G

G

C

C

H

H

L
x
I

N
|
N

P

P

A

A

V

V

T

T

V

I

G

G

L

L

W

W

S

S

G

V

G

K

R

K

F

F

P

P

A

G

A

R

D

E

V

V

P

P

Y

Y

I

I

L

I

A

A

Q

Q

V

L

L

L

G

G

A

A

I

A

V

F

G

G

A

S

F

F

V

I

L

F

Y

L

I

Q

I

C

A

A

S

G

G

-

Q

-

A

-

G

-

F

-

E

-

L

-

A

I

G

G

G

A

L

A

A

T

S

V

N

G

G

G

Y

L

G
|
G

E
x
A

H

T

S
x
A

P
|
P

-
x
F

G

P

G

G

Y

I

T

S

L

Y

M

W

S

Q

G

A

F

M

V

L

T

A

E

E

F

V

V

V

M

G

T

T

F

F

M

L

F

L

L

M

I

I

V

T

I

I

L

M

G

G

-

I

A

A

T

V

H

D

K

E

L

R

A

A

T

P

P

K

A

G

M

F

A

A

G

G

L

I

A

I

I

I

G

G

L

L

A

T

L

V

T

A

L

G

I

I

H

I

L

T

I

T

S

L

I

G

P

N

V

I

T

S

N

G

T

S

S

S

V

L

N

N

P

P

A

A

R

R

S

T

T

F

G

G

P

P

A

Y

L

L

M

N

V

D

G

M

D

I

W

F

A

A

V

G

A

T

Q

D

L

L

W

W

L

N

F

Y

W

Y

-

S

-

I

-

Y

-

V

L

I

A

G

P

P

L

I

L

V

G

G

A

A

L

V

V

L

A

A

G

A

I

L

V

T

Y

Y

R

Q

W

Y

L

L

S

T

binding glycerol: I44 (vs. gap), W55 (≠ L36), I66 (≠ V47), I81 (≠ L62), N82 (= N63), G133 (= G121), A134 (≠ E122), A136 (≠ S124), P137 (= P125), F138 (vs. gap)

P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
35% identity, 99% coverage: 2:229/231 of query aligns to 35:255/323 of P47863

query
sites
P47863

F

W

K

K

K

A

L

V

V

T

A

A

E

E

F

F

I

L

G

A

T

M

F

L

W

I

L

F

V

V

L

L

G

L

G

S

C

V

G

G

S

S

A

T

V

I

L

N

-

W

A

G

A

S

F

E

P

N

E

P

V

L

G

P

I

V

G

D

L

M

L

V

G

L

V

I

S

S

L

L

A

C

F

F

G

G

L

L

T

S

V

I

V

A

T

T

M

M

A

V

Y

Q

A

C

I

F

G

G

H

H

I

I

S

S

G

G

C

G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

V

G

A

L

M

W

V

S

C

G

T

G

R

R

K

F

I

P

S

A

I

A

A

D

K

V

S

V

V

P

F

Y

Y

I

I

L

T

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

V

I

G

G

A

A

F

G

V

I

L

L

Y

Y

I

L

I

V

A

T

S

P

G

P

Q

S

A

-

G

-

F

-

E

-

L

V

A

V

G

G

L

L

A

G

S

V

N

T

G

T

Y

V

G

-

E

-

H

-

S

-

P

H

G

G

G

N

Y

L

T

T

L

A

M

G

S

H

G

G

F

L

V

L

T

V

E

E

F

L

V

I

M

I

T

T

F

F

M

Q

F

L

L

V

I

F

V

T

I

I

L

F

G

A

A

S

T

C

H

D

K
x
S

L

K

A

R

T

T

P

D

A

V

M

T

A

G

G

S

-

V

-

A

L

L

A

A

I

I

G

G

L

F

A

S

L

V

T

A

L

I

I

G

H
|
H

L

L

I

F

S

A

I

I

P

N

V

Y

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

M

I

V

M

G

G

D

N

W

W

A

-

V

-

A

E

Q

N

L

H

W

W

L

I

F

Y

W

W

L

V

A

G

P

P

L

I

G

G

A

A

L

V

V

L

A

A

G

G

I

A

V

L

Y

Y

R

E

W

Y

L

V

S

F

P

C

Q

P

D

D

S180 (≠ K154) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.
H201 (= H173) mutation to P: Partial loss of transport activity.

Sites not aligning to the query:

13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
285 modified: Phosphoserine

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
34% identity, 97% coverage: 3:225/231 of query aligns to 11:222/271 of P56402

query
sites
P56402

K

R

K

A

L

V

V

L

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

L

L

F

V

V

L

F

G

F

G

G

C

L

G

G

S

S

A

A

V

L

L

Q

A

W

A

A

A

S

F

S

P

P

E

P

V

-

G

-

I

-

G

S

L

V

L

L

G

Q

V

I

S

A

L

V

A

A

F

F

G

G

L

L

T

G

V

I

V

G

T

T

M

L

A

V

Y

Q

A

A

I

L

G

G

H

H

I

V

S

S

G

G

C

A

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

V

G

A

L

C

W

L

S

V

G

G

G

C

R

H

F

V

P

S

A

F

A

L

D

R

V

A

P

F

Y

Y

I

V

L

A

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

V

A

G

G

A

A

F

A

V

I

L

L

Y

H

I

E

I

I

A

T

S

P

G

-

Q

-

A

-

G

-

F

V

E

E

L

I

A

R

G

G

G

D

L

L

A

A

S

V

N

N

G

A

Y

L

G

H

E

N

H

N

S

A

P
x
T

G

A

G

G

Y

Q

T

A

L

V

M

-

S

-

G

-

F

-

V

T

T

V

E

E

F

L

V

F

M

L

T

T

F

M

M

Q

F

L

L

V

I

L

V

C

I

I

L

F

G

A

A

S

T

T

H

D

K

E

L

R

A

R

T

S

P

D

A

N

M

L

A

G

G

S

-

P

-

A

L

L

A

S

I

I

G

G

L

F

A

S

L

V

T

T

L

L

I

G

H

H

L

L

I

L

S

G

I

I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

A

P

P

A

A

L

V

M

V

V

T

G

G

D

K

W

F

A

-

V

-

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

I

A

G

P

P

L

L

L

V

G

G

A

A

L

V

V

I

A

G

G

S

I

L

V

L

Y

Y

R

N

W

Y

L

L

T126 (≠ P125) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

4nefA X-ray structure of human aquaporin 2 (see paper)
33% identity, 97% coverage: 3:225/231 of query aligns to 10:221/239 of 4nefA

query
sites
4nefA

K

R

K

A

L

V

V

F

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

L

L

F

V

V

L

F

G

F

G

G

C

L

G

G

S

S

A

A

V

L

L

-

A

-

A

N

F

W

P

P

E

Q

V

A

G

L

I

P

G

S

L

V

L

L

G

Q

V

I

S

A

L

M

A

A

F

F

G

G

L

L

T

G

V

I

V

G

T

T

M

L

A

V

Y

Q

A

A

I

L

G

G

H

H

I

I

S

S

G

G

C

A

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

V

G

A

L
x
C

W

L

S

V

G

G

G

C

R
x
H

F

V

P

S

A

V

A

L

D

R

V

A

P

F

Y

Y

I

V

L

A

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

V

A

G

G

A

A

F

A

V

L

L

L

Y

H

I

E

I

I

A

T

S

P

G

A

Q

-

A

-

G

-

F

-

E

D

L

I

A

R

G

G

G

D

L

L

A

A

S

V

N

N

G

A

Y

L

G

S

E

N

H

S

S

T

P

T

G

A

G

G

Y

Q

T

A

L

V

M

-

S

-

G

-

F

-

V

T

T

V

E

E

F

L

V

F

M

L

T

T

F

L

M

Q

F

L

L

V

I

L

V

C

I

I

L

F

G

A

A

S

T

T

H

D

K

E

L

R

-

R

-

G

A

E

T

N

P

P

A

G

M

T

A

P

G

A

L

L

A

S

I

I

G

G

L

F

A

S

L

V

T

A

L

L

I

G

H

H

L

L

I

L

S

G

I

I

P

H

V

Y

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

A

P

P

A

A

L

V

M

V

V

T

G

G

D

K

W

F

A

-

V

-

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

I

A

G

P

P

L

L

L

V

G

G

A

A

L

I

V

L

A

G

G

S

I

L

V

L

Y

Y

R

N

W

Y

L

V

binding zinc ion: C74 (≠ L70), H79 (≠ R75)

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 2 papers)
37% identity, 97% coverage: 3:225/231 of query aligns to 12:224/265 of P55064

query
sites
P55064

K

K

K

A

L

V

V

F

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

I

L

F

V

V

L

F

G

F

G

G

C

L

G

G

S

S

A

A

V

L

L

-

A

-

A

K

F

W

P

P

E

S

V
x
A

G

L

I

P

G

T

L

I

L

L

G

Q

V
x
I

S

A

L

L

A

A

F

F

G

G

L

L

T

A

V

I

V

G

T

T

M

L

A

A

Y

Q

A

A

I

L

G

G

H

P

I

V

S

S

G

G

C

G

H

H

L

I

N

N

P

P

A

A

V

I

T

T

V

L

G

A

L

L

W

L

S

V

G

G

G

N

R

Q

F

I

P

S

A

L

D

R

V

A

V

F

P

F

Y

Y

I

V

L

A

A

A

Q

Q

V

L

L

V

G

G

A

A

I

I

V

A

G

G

A

A

F

G

V

I

L

L

Y

Y

I

-

A

-

S

-

G

G

Q

V

A

A

G

P

F

L

E

N

L

A

A

R

G

G

G

N

L

L

A

A

S

V

N

N

G

A

Y

L

G
x
N

E

N

H

N

S

T

P

T

G

Q

G

G

Y

Q

T

A

L

M

M

-

S

-

G

-

F

-

V

V

T

V

E

E

F

L

V

I

M

L

T

T

F

F

M

Q

F

L

L

A

I

L

V

C

I

I

L

F

G

A

A

S

T

T

H

D

K

S

L

R

A

R

T

T

P
x
S

A

P

M

V

A

G

G

S

-

P

-

A

L

L

A

S

I

I

G

G

L

L

A

S

L

V

T

T

L

L

I

G

H

H

L

L

I

V

S

G

I
|
I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R
|
R

S

S

T

F

G

G

P

P

A

A

L

V

M

V

V

M

G

N

D

R

W

F

A

S

V

P

A

A

Q

H

L

-

W

W

L

V

F

F

W

W

L

V

A

G

P

P

L

I

L

V

G

G

A

A

L

V

V

L

A

A

G

A

I

I

V

L

Y

Y

R

F

W

Y

L

L

A38 (≠ V32) to E: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123054
I45 (≠ V39) to S: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123055
N123 (≠ G121) to D: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123057
S156 (≠ P158) mutation to A: No effect on location at the cell membrane.; mutation to E: Increased location at the cell membrane.
I177 (= I177) to F: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123056
R188 (= R188) to C: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs368292687

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
33% identity, 97% coverage: 3:225/231 of query aligns to 11:222/271 of P41181

query
sites
P41181

K

R

K

A

L

V

V

F

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

L

L

F

V

V

L

F

G

F

G

G

C

L

G

G

S

S

A

A

V

L

L

-

A

-

A

N

F

W

P

P

E

Q

V

A

G

L

I

P

G

S

L

V

L

L

G

Q

V

I

S

A

L

M

A

A

F

F

G

G

L

L

T

G

V

I

V

G

T

T

M

L

A

V

Y

Q

A

A

I

L

G

G

H

H

I

I

S

S

G
|
G

C

A

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

V

G

A

L

C

W

L

S

V

G
|
G

G
x
C

R

H

F

V

P

S

A

V

A

L

D

R

V

A

P

F

Y

Y

I

V

L

A

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

V

A

G

G

A

A

F

A

V

L

L

L

Y

H

I

E

I

I

A

T

S

P

G

A

Q

-

A

-

G

-

F

-

E

D

L

I

A

R

G

G

G

D

L

L

A

A

S

V

N

N

G

A

Y

L

G

S

E

N

H

S

S

T

P

T

G

A

G

G

Y

Q

T

A

L

V

M

-

S

-

G

-

F

-

V

T

T

V

E

E

F

L

V

F

M

L

T

T

F

L

M

Q

F

L

L

V

I

L

V

C

I

I

L

F

G

A

A
x
S

T

T

H

D

K

E

L

R

-

R

-

G

A

E

T

N

P

P

A

G

M

T

A

P

G

A

L

L

A

S

I

I

G

G

L

F

A

S

L

V

T

A

L

L

I

G

H

H

L

L

I

L

S

G

I

I

P

H

V

Y

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R
|
R

S

S

T

L

G
x
A

P

P

A

A

L

V

M
x
V

V

T

G

G

D

K

W

F

A

-

V

-

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

I

A

G

P

P

L

L

L

V

G

G

A

A

L

I

V

L

A

G

G
x
S

I
x
L

V

L

Y

Y

R

N

W
x
Y

L

V

G64 (= G59) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (= G73) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ G74) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
S148 (≠ A151) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R188) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G191) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ M195) to I: in dbSNP:rs772051028
S216 (≠ G219) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
L217 (≠ I220) mutation to A: Abolishes interaction with MIAC; when associated with A-221.
Y221 (≠ W224) mutation to A: Abolishes interaction with MIAC; when associated with A-217.

Sites not aligning to the query:

229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
232 E→A: Reduces interaction with MIAC.
244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
37% identity, 97% coverage: 3:225/231 of query aligns to 11:223/253 of 5dyeD

query
sites
5dyeD

K

K

K

A

L

V

V

F

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

I

L

F

V

V

L

F

G

F

G

G

C

L

G

G

S

S

A

A

V

L

L

-

A

-

A

K

F

W

P

P

E

S

V

A

G

L

I

P

G

T

L

I

L

L

G

Q

V

I

S

A

L

L

A

A

F

F

G

G

L

L

T

A

V

I

V

G

T

T

M

L

A

A

Y

Q

A

A

I

L

G

G

H

P

I

V

S

S

G

G

C

G

H

H

L

I

N

N

P

P

A

A

V

I

T

T

V

L

G

A

L

L

W

L

S

V

G

G

G

N

R

Q

F

I

P

S

A

L

D

R

V

A

V

F

P

F

Y

Y

I

V

L

A

A

A

Q

Q

V

L

L

V

G

G

A

A

I

I

V

A

G

G

A

A

F

G

V

I

L

L

Y

Y

I

-

A

-

S

-

G

G

Q

V

A

A

G

P

F

L

E

N

L

A

A

R

G

G

G

N

L

L

A

A

S

V

N

N

G

A

Y

L

G

N

E

N

H

N

S

T

P

T

G

Q

G

G

Y

Q

T

A

L

M

M

-

S

-

G

-

F

-

V

V

T

V

E

E

F

L

V

I

M

L

T

T

F

F

M

Q

F

L

L

A

I

L

V

C

I

I

L

F

G

A

A

S

T

T

H

D

K

S

L

R

A

R

T

T

P

E

A

P

M

V

A
x
G

G
x
S

-

P

-

A

L

L

A

S

I

I

G

G

L

L

A

S

L

V

T

T

L

L

I

G

H

H

L

L

I

V

S

G

I

I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

M

V

V

M

G

N

D

R

W

F

A

S

V

P

A

A

Q

H

L

-

W

W

L

V

F

F

W

W

L

V

A

G

P

P

L

I

L

V

G

G

A

A

L

V

V

L

A

A

G

A

I

I

V

L

Y

Y

R

F

W

Y

L

L

binding O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine: G158 (≠ A161), S159 (≠ G162)

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 100% coverage: 1:231/231 of query aligns to 78:295/305 of Q9SAI4

query
sites
Q9SAI4

M

L

F

Y

K

R

K

K

L

L

V

G

A

A

E

E

F

F

I

V

G

G

T

T

F

L

W

I

L

L

V

I

L

F

G

A

G

G

C

T

G

A

S

T

A

A

V

I

L

V

A

N

A

Q

A

K

F

T

P

-

E

D

V

G

G

A

I

E

G

T

L

L

L

I

G

G

V

C

S

A

L

A

A

S

F
x
A

G

G

L

L

T

A

V

V

V

M

T

I

M

V

A

I

Y

L

A

S

I

T

G

G

H

H

I

I

S

S

G

G

C

A

H

H

L

L

N

N

P

P

A

A

V

V

T

T

V

I

G

A

L

F

W

A

S

A

G

L

G

K

R

H

F

F

P

P

A

W

A

K

D

H

V

V

V

P

P

V

Y

Y

I

I

L

G

A

A

Q

Q

V

V

L

M

G

A

A

S

I

V

V

S

G

A

A

A

F

F

V

A

L

L

Y

-

I

-

A

-

S

-

G

-

Q

K

A

A

G

V

F

F

E

E

-

P

-

T

L

M

A

S

G

G

L

V

A

T

S

V

N

P

G

T

Y

V

G

G

E

-

H

-

S

-

P

-

G

-

Y

-

T

-

L

L

M

S

S

Q

G

A

F

F

V

A

T

L

E

E

F

F

V

I

M

I

T

S

F

F

M

N

F

L

L

M

I

F

V

V

I

V

L

T

G

A

-

V

A

A

T

T

H

D

K

T

L

R

A

A

T

V

P

G

A

E

M

L

A

A

G

G

L

I

A

A

I

V

G

G

L

A

A

T

L

V

T

M

L

L

I

N

H

I

L

L

I

I

S

A

I

G

P

P

V

A

T

T

N

S

T

A

S

S

V

M

N

N

P

P

A
x
V

R

R

S

T

T

L

G

G

P

P

A

A

L

I

M

A

V

A

G

N

D

N

W

Y

A

R

V

A

A

-

Q

-

L

I

W

W

L

V

F

Y

W

L

L

T

A

A

P

P

L

I

L

L

G

G

A

A

L

L

V

I

A

G

G

A

I

G

V

T

Y

Y

R

T

W

I

L

V

S

K

-

L

P

P

Q

E

D

E

D

D

D

E

A119 (≠ F43) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.
V252 (≠ A187) mutation to A: No effect.

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
31% identity, 97% coverage: 1:225/231 of query aligns to 35:269/285 of Q9XF58

query
sites
Q9XF58

M

L

F

Y

K

R

K

A

L

V

V

I

A

A

E

E

F

F

I

V

G

A

T

T

F

L

W

L

L

F

-

L

-

Y

-

I

-

T

-

V

-

A

-

T

V

V

L

I

G

G

G

Y

C

K

G

H

S

Q

A

T

V

D

L

A

A

A

A

S

F

G

P

P

E

D

V

A

-

A

-

C

-

G

G

G

I

V

G

G

L

V

L

L

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

V

F

T

I

M

L

A

V

Y

Y

A

C

I

T

G

A

H

G

I

V

S

S

G

G

C
x
G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

F

G

G

L

L

W

F

S

L

G

A

G

R

R

K

F

V

P

S

A

L

A

V

D

R

V

A

V

L

P

L

Y

Y

I

I

L

V

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

V

C

G

G

A

V

F

G

V

L

L

V

Y

-

I

-

A

-

S

-

G

-

Q

-

A

K

G

G

F

F

E

Q

L

S

A

A

G

F

G

Y

L

V

A

R

S

Y

N

G

G

G

Y

G

G

A

E

N

H

E

S

L

P

S

G

A

G

G

Y

Y

T

S

L

K

M

G

S

T

G

G

F

L

V

A

T

A

E

E

F

I

V

I

M

G

T

T

F

F

M

V

F

L

L

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

H

D

K

P

L

K

A

R

T

N

-

A

-

R

-

D

-

S

-

H

-

V

P

P

A

V

M

L

A

A

G

P

L

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

M

I

V

H

H

L

L

I

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

L

V

M

I

V

Y

-

N

G

N

D

D

W

K

A

A

V

W

A

D

Q

D

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

L

F

L

I

G

G

A

A

L

A

V

I

A

A

G

A

I

A

V

Y

Y

H

R

Q

W

Y

L

V

G104 (≠ C60) mutation to W: Loss of water transport.

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
35% identity, 99% coverage: 3:231/231 of query aligns to 10:235/247 of 8ct2D

query
sites
8ct2D

K

R

K

A

L

V

V

V

A

A

E

E

F

F

I

L

G

A

T

T

F

T

W

L

L

F

V

V

L

F

G

I

G

S

C

I

G

G

S

S

A

A

V

-

L

L

A

G

A

F

A

K

F

Y

P

P

E

-

V

V

G

G

I

N

G

N

L

Q

-

T

-

A

-

V

-

Q

-

D

-

N

L

V

G

K

V

V

S

S

L

L

A

A

F

F

G

G

L

L

T

S

V

I

V

A

T

T

M

L

A

A

Y

Q

A

S

I

V

G

G

H

H

I

I

S

S

G

G

C

A

H

H

L

L

N

N

P

P

A

A

V

V

T

T

V

L

G

G

L

L

W

L

S

L

G

S

G

C

R

Q

F

I

P

S

A

I

A

F

D

R

V

A

V

L

P

M

Y

Y

I

I

L

I

A

A

Q

Q

V

C

L

V

G

G

A

A

I

I

V

V

G

A

A

T

F

A

V

I

L

L

Y

S

I

G

I

I

A

T

S

S

G

S

Q

L

A

T

G

G

F

N

E

S

L

L

A

G

G

R

G

N

L

D

A

L

S

A

N

D

G

G

Y

V

G

N

E

S

H
x
G

S

-

P

-

G

-

Y

-

T

-

L

-

M

-

S

Q

G

G

F

L

V
x
G

T

I

E

E

F

I

V

I

M

G

T

T

F

L

M

Q

F

L

L

V

I

L

V

C

I

V

L

L

G

A

A

T

T

T

H

D

K

R

L

R

A

R

T

R

P

D

-

L

-

G

A

G

M

S

A

A

G

P

L

L

A

A

I

I

G

G

L

L

A

S

L

V

T

A

L

L

I

G

H

H

L

L

I

L

S

A

I

I

P

D

V

Y

T

T

N

G

T

C

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

S

A

A

L

V

M

I

V

T

G

H

D

N

W

F

A

-

V

-

A

S

Q

N

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

L

F

L

I

G

G

A

G

L

A

V

L

A

A

G

V

I

L

V

I

Y

Y

R

D

W

F

-

I

L

L

S

A

P

P

Q

R

D

S

D

D

binding cholesterol: G134 (≠ H123), G138 (≠ V135)

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 94% coverage: 6:222/231 of query aligns to 55:273/286 of Q06611

query
sites
Q06611

V

I

A

A

E

E

F

F

I

I

G

A

T

T

F

F

W

L

L

F

-

L

-

Y

-

I

-

T

-

V

-

L

-

T

V

V

L

M

G

G

G

V

C

K

G

R

S

S

A

P

V

N

L

M

A

C

A

A

A

S

F

-

P

-

E

-

V

-

G

-

I

V

G

G

L

I

L

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

V

F

T

A

M

L

A

V

Y

Y

A

C

I

T

G

A

H

G

I

I

S

S

G

G

C

G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

V

F

G

G

L

L

W

F

S

L

G

A

G

R

R

K

F

L

P

S

A

L

A

T

D

R

V

A

V

V

P

Y

Y

Y

I

I

L

V

A

M

Q

Q

V

C

L

L

G

G

A

A

I

I

V

C

G

G

A

A

F

G

V

V

L

V

Y

-

I

-

A

-

S

-

G

-

Q

-

A

K

G

G

F

F

E

Q

L

P

A

K

G

Q

G

Y

L

Q

A

A

S

L

N

G

G

G

Y

G

G

A

E

N

H

T

S

I

P

A

G

H

G

G

Y

Y

T

T

L

K

M

G

S

S

G

G

F

L

V

G

T

A

E

E

F

I

V

I

M

G

T

T

F

F

M

V

F

L

L

V

I

Y

V

T

I

V

L

F

G

S

A

A

T

T

H

D

K

A

L

K

A

R

T

N

-

A

-

R

-

D

-

S

-

H

-

V

P

P

A

I

M

L

A

A

G

P

L

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

L

I

V

H

H

L

L

I

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

L

I

M

I

V

F

G

N

-

K

D

D

W

N

A

A

V

W

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

V

A

G

P

P

L

F

L

I

G

G

A

A

L

A

V

L

A

A

G

A

I

L

V

Y

Y

H

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 99% coverage: 1:229/231 of query aligns to 47:261/298 of Q6Z2T3

query
sites
Q6Z2T3

M

L

F

L

K

K

L

V

V

V

A

S

E

E

F

V

I

V

G

A

T

T

F

F

W

L

L

L

V

V

L

F

G

M

G

T

C

C

G

G

S

A

A

A

V

G

L

I

A

S

A

G

A

S

F

-

P

D

E

L

V

S

G

R

I

I

G

S

L

Q

L

L

G

G

V

Q

S

S

L

I

A

A

F

G

G

G

L

L

T

I

V

V

V

T

T

V

M

M

A

I

Y

Y

A

A

I

V

G

G

H

H

I

I

S

S

G

G

C

A

H

H

L

M

N

N

P

P

A

A

V

V

T

T

V

L

G

A

L

F

W

A

S

V

G

F

G

R

R

H

F

F

P

P

A

W

A

I

D

Q

V

V

V

P

P

F

Y

Y

I

W

L

A

A
|
A

Q

Q

V

F

L

T

G

G

A

A

I

I

V

C

G

A

A

S

F

F

V

V

L

L

Y

K

I

A

I

V

A

I

S

H

G

-

Q

-

A

-

G

P

F

V

E

D

L

V

A

I

G

G

G

T

L

T

A

T

S

P

N

V

G

G

Y

P

G

H

E

W

H

H

S

S

P

-

G

-

Y

-

T

-

L

-

M

-

S

-

G

-

F

L

V

V

T

V

E

E

F

V

V

I

M

V

T

T

F

F

M

N

F

M

L

M

I

F

V

V

I

T

L

L

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A132 (= A87) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 97% coverage: 1:223/231 of query aligns to 50:274/286 of Q08733

query
sites
Q08733

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Sites not aligning to the query:

1 modified: N-acetylmethionine

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 94% coverage: 6:222/231 of query aligns to 55:273/286 of P61837

query
sites
P61837

V

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Sites not aligning to the query:

1 modified: N-acetylmethionine

8sjxA Structure of lens aquaporin-0 array in sphingomyelin/cholesterol bilayer (2sm:1chol)
32% identity, 97% coverage: 3:225/231 of query aligns to 6:217/220 of 8sjxA

query
sites
8sjxA

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binding cholesterol: I82 (≠ V82), F193 (≠ W199), W200 (= W208)
binding [(E,2S,3R)-2-(hexadecanoylamino)-3-oxidanyl-octadec-4-enyl] 2-(trimethylazaniumyl)ethyl phosphate: F9 (≠ V6), F9 (≠ V6), F12 (= F9), V86 (≠ L86), V86 (≠ L86), L89 (≠ V89), L90 (= L90), L90 (= L90), L90 (= L90), Y100 (= Y100), Y100 (= Y100), S101 (≠ I101), I188 (≠ L194), L189 (≠ M195), R191 (≠ G197), F193 (≠ W199)

Sites not aligning to the query:

binding [(E,2S,3R)-2-(hexadecanoylamino)-3-oxidanyl-octadec-4-enyl] 2-(trimethylazaniumyl)ethyl phosphate: 5, 5

Query Sequence

>WP_068332332.1 NCBI__GCF_001650345.1:WP_068332332.1
MFKKLVAEFIGTFWLVLGGCGSAVLAAAFPEVGIGLLGVSLAFGLTVVTMAYAIGHISGC
HLNPAVTVGLWSGGRFPAADVVPYILAQVLGAIVGAFVLYIIASGQAGFELAGGLASNGY
GEHSPGGYTLMSGFVTEFVMTFMFLIVILGATHKLATPAMAGLAIGLALTLIHLISIPVT
NTSVNPARSTGPALMVGDWAVAQLWLFWLAPLLGALVAGIVYRWLSPQDDD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory