SitesBLAST
Comparing WP_068464614.1 NCBI__GCF_001541235.1:WP_068464614.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
37% identity, 96% coverage: 1:436/455 of query aligns to 1:459/485 of Q8DLI5
- R6 (= R6) binding L-glutamate
- Y192 (= Y194) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
37% identity, 96% coverage: 2:436/455 of query aligns to 1:458/484 of 2cfoA
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
34% identity, 99% coverage: 1:450/455 of query aligns to 1:457/468 of 8i9iA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
34% identity, 99% coverage: 1:450/455 of query aligns to 1:457/471 of P04805
- C98 (= C97) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (≠ E99) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (vs. gap) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ A123) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ L125) mutation to Q: No change in activity or in zinc content.
- H131 (≠ I127) mutation to Q: No change in activity or in zinc content.
- H132 (≠ T128) mutation to Q: No change in activity or in zinc content.
- C138 (≠ T134) mutation to S: No change in activity or in zinc content.
- S239 (= S252) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
36% identity, 71% coverage: 3:324/455 of query aligns to 3:296/380 of 4g6zA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
33% identity, 73% coverage: 2:332/455 of query aligns to 1:333/485 of 4griB
- active site: S9 (= S10), K253 (= K253)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (≠ D42), Y194 (= Y194), R212 (= R212), W216 (≠ H216)
- binding zinc ion: C105 (= C97), C107 (≠ E99), Y128 (= Y120), C132 (≠ A124)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
34% identity, 74% coverage: 3:338/455 of query aligns to 103:420/564 of 3al0C
- active site: S110 (= S10), K335 (= K253)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R6), A108 (= A8), P109 (= P9), G118 (= G18), T122 (= T22), E142 (≠ D42), Y276 (= Y194), R294 (= R212), G295 (= G213), D297 (= D215), H298 (= H216), L324 (= L242), I325 (≠ L243), L333 (= L251)
- binding : T144 (= T44), D145 (= D45), R148 (= R48), Y208 (= Y120), P213 (≠ E131), K252 (≠ R170), M255 (≠ Q173), I266 (≠ V184), K269 (≠ R187), S270 (≠ A188), Y276 (= Y194), D297 (= D215), H298 (= H216), L299 (≠ V217), S300 (≠ T218), N301 (= N219), K304 (≠ V222), R330 (≠ G248), P332 (≠ A250), G363 (= G281), W364 (≠ T282), R365 (≠ S283), E370 (≠ A288), S387 (= S305), K389 (≠ A307), V391 (≠ A309), I392 (≠ R310), K397 (≠ E315), W400 (≠ S318), R407 (≠ H325)
Sites not aligning to the query:
- binding : 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid
28% identity, 99% coverage: 2:450/455 of query aligns to 2:494/502 of 6brlA
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 2cv2A
- active site: K246 (= K253)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R6), A7 (= A8), S9 (= S10), G17 (= G18), I21 (≠ T22), E41 (≠ D42), Y187 (= Y194), R205 (= R212), A206 (≠ G213), E208 (≠ D215), W209 (≠ H216), L235 (= L242), L236 (= L243)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (≠ R145), R163 (= R170), Y168 (≠ V175), E172 (≠ S179), V177 (= V184), K180 (≠ R187), S181 (≠ A188), Y187 (= Y194), E207 (= E214), E208 (≠ D215), W209 (≠ H216), V211 (≠ T218), R237 (≠ V244), K241 (≠ G248), L272 (= L279), M273 (≠ I280), G274 (= G281), E282 (= E287), S299 (= S305), P303 (≠ A309), V304 (≠ R310), K309 (≠ E315), W312 (≠ S318), R319 (≠ H325), P357 (≠ G353), R358 (≠ N354), R417 (≠ A404), Q432 (≠ H419), R435 (= R422), L442 (≠ N429), E443 (≠ D430), T444 (≠ G431), G446 (≠ E433), L447 (= L434), F448 (≠ K435)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 2cv1A
- active site: K246 (= K253)
- binding adenosine-5'-triphosphate: P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ N19), I21 (≠ T22), R47 (= R48), A206 (≠ G213), W209 (≠ H216), L235 (= L242), L236 (= L243)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R6), A7 (= A8), E41 (≠ D42), Y187 (= Y194), R205 (= R212), W209 (≠ H216)
- binding : S9 (= S10), E41 (≠ D42), T43 (= T44), D44 (= D45), R47 (= R48), V145 (≠ R145), R163 (= R170), V166 (≠ Q173), E172 (≠ S179), V177 (= V184), K180 (≠ R187), S181 (≠ A188), Y187 (= Y194), E207 (= E214), E208 (≠ D215), W209 (≠ H216), V211 (≠ T218), R237 (≠ V244), K241 (≠ G248), K243 (≠ A250), M273 (≠ I280), G274 (= G281), S276 (= S283), E282 (= E287), S299 (= S305), P303 (≠ A309), V304 (≠ R310), K309 (≠ E315), W312 (≠ S318), R319 (≠ H325), P357 (≠ G353), R358 (≠ N354), R417 (≠ A404), L427 (≠ G414), Q432 (≠ H419), R435 (= R422), L442 (≠ N429), E443 (≠ D430), T444 (≠ G431), G446 (≠ E433), L447 (= L434), F448 (≠ K435)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 1n78A
- active site: K246 (= K253)
- binding glutamol-amp: R5 (= R6), A7 (= A8), P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ N19), I21 (≠ T22), E41 (≠ D42), Y187 (= Y194), N191 (≠ S198), R205 (= R212), A206 (≠ G213), E208 (≠ D215), W209 (≠ H216), L235 (= L242), L236 (= L243)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (≠ R145), R163 (= R170), V166 (≠ Q173), Y168 (≠ V175), E172 (≠ S179), V177 (= V184), K180 (≠ R187), S181 (≠ A188), Y187 (= Y194), E207 (= E214), E208 (≠ D215), W209 (≠ H216), L210 (≠ V217), V211 (≠ T218), R237 (≠ V244), K241 (≠ G248), M273 (≠ I280), G274 (= G281), E282 (= E287), R297 (≠ K303), P303 (≠ A309), V304 (≠ R310), K309 (≠ E315), W312 (≠ S318), R319 (≠ H325), P357 (≠ G353), R358 (≠ N354), R417 (≠ A404), L427 (≠ G414), Q432 (≠ H419), R435 (= R422), L442 (≠ N429), E443 (≠ D430), T444 (≠ G431), G446 (≠ E433), L447 (= L434), F448 (≠ K435)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 1j09A
- active site: K246 (= K253)
- binding adenosine-5'-triphosphate: H15 (= H16), E208 (≠ D215), L235 (= L242), L236 (= L243), K243 (≠ A250), I244 (≠ L251), S245 (= S252), K246 (= K253), R247 (= R254)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (≠ D42), Y187 (= Y194), N191 (≠ S198), R205 (= R212), W209 (≠ H216)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of P27000
- R358 (≠ N354) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
30% identity, 98% coverage: 3:450/455 of query aligns to 2:463/468 of 1g59A
- binding : D44 (= D45), R45 (≠ L46), A46 (≠ Q47), R47 (= R48), P109 (≠ E101), V145 (≠ R145), R163 (= R170), V166 (≠ Q173), E172 (≠ S179), V177 (= V184), K180 (≠ R187), S181 (≠ A188), D182 (= D189), E207 (= E214), E208 (≠ D215), R237 (≠ V244), K241 (≠ G248), T242 (≠ A249), K243 (≠ A250), M273 (≠ I280), G274 (= G281), E282 (= E287), S299 (= S305), L300 (≠ T306), P303 (≠ A309), V304 (≠ R310), K309 (≠ E315), W312 (≠ S318), R319 (≠ H325), P357 (≠ G353), R358 (≠ N354), R417 (≠ A404), K426 (= K413), L427 (≠ G414), Q432 (≠ H419), R435 (= R422), L442 (≠ N429), E443 (≠ D430), T444 (≠ G431), P445 (= P432), G446 (≠ E433), L447 (= L434), F448 (≠ K435)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
31% identity, 79% coverage: 2:362/455 of query aligns to 3:361/488 of 8vc5A
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
30% identity, 62% coverage: 2:285/455 of query aligns to 10:282/455 of 3aiiA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
32% identity, 56% coverage: 6:258/455 of query aligns to 7:234/290 of 4a91A
- active site: S11 (= S10), K229 (= K253)
- binding glutamic acid: R7 (= R6), A9 (= A8), S11 (= S10), E43 (≠ D42), Y170 (= Y194), R188 (= R212), L192 (≠ H216)
- binding zinc ion: C99 (= C97), C101 (≠ E99), Y113 (= Y120), C117 (≠ A124)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
32% identity, 55% coverage: 6:254/455 of query aligns to 19:242/308 of P27305
- E55 (≠ D42) binding L-glutamate
- Y182 (= Y194) binding L-glutamate
- R200 (= R212) binding L-glutamate
4h3sA The structure of glutaminyl-tRNA synthetase from saccharomyces cerevisiae (see paper)
34% identity, 25% coverage: 3:117/455 of query aligns to 38:148/585 of 4h3sA
Sites not aligning to the query:
Query Sequence
>WP_068464614.1 NCBI__GCF_001541235.1:WP_068464614.1
MSVKVRFAPSPTGRLHVGNVRTALLNWLFARNSGGTFMLRMDDTDLQRSTAEFADGIRVD
LNWLGLRWDEEKRQSDRTDRYAAAAEALKAQGRLYACYETEDELDRKRKRQRARGMPPIY
DRAALKITPDEHATLAAEGRRPHWRFRLDNSENGSLTPLPTIVSWNDLIRGDQTVDVGSL
SDPVVIRADGSFLYTFTSVIDDAEFGITHIIRGEDHVTNTGVQLQLFEALGAVPPAFGHH
SLLVGADGAALSKRLGALSIESLREDGIEPMAVSSYTALIGTSDAIEAVADIDDLAARFA
FSKISTAPARFDPEELKSLNARLLHATPYDDVAAHLADLGVGGDAAFWLAVRGNISVLAD
AAIWWRVVADRIDPVIEDRELTDIAAGLLPAEPWCDTTWDDWIAKVKAASGRKGRGLFHP
LRMALTGRNDGPELKALLPLIGRARAEARLRGQQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory