SitesBLAST

8a6tA Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
38% identity, 74% coverage: 3:182/243 of query aligns to 6:207/571 of 8a6tA

query
sites
8a6tA

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binding fe2/s2 (inorganic) cluster: C36 (= C33), D37 (≠ H34), C47 (= C44), R48 (= R45), C50 (= C47), C63 (= C63)
binding iron/sulfur cluster: H95 (≠ A95), C99 (vs. gap), C102 (vs. gap), C108 (≠ I101), C146 (= C124), C149 (= C127), G150 (= G128), K151 (≠ L129), C152 (= C130), C156 (= C134), C189 (= C164), C192 (= C167), C195 (= C170), C199 (= C174), G202 (= G177)

Sites not aligning to the query:

binding 2 iron/2 sulfur/5 carbonyl/2 water inorganic cluster: 229, 230, 231, 298, 323, 352, 353, 357, 412, 500
binding iron/sulfur cluster: 299, 354, 496, 500

8a5eA Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
34% identity, 79% coverage: 1:192/243 of query aligns to 4:217/583 of 8a5eA

query
sites
8a5eA

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binding fe2/s2 (inorganic) cluster: T34 (= T31), C36 (= C33), G45 (= G42), C47 (= C44), C50 (= C47), C64 (= C63)
binding iron/sulfur cluster: H96 (≠ A95), N97 (vs. gap), C100 (vs. gap), C103 (vs. gap), S106 (≠ P96), C109 (≠ E99), C148 (= C124), C151 (= C127), K152 (≠ G128), C154 (= C130), C158 (= C134), V166 (≠ A141), C191 (= C164), C194 (= C167), G195 (= G168), C197 (= C170), C201 (= C174), P202 (= P175), V203 (≠ T176)

Sites not aligning to the query:

7vw6A Cryo-em structure of formate dehydrogenase 1 from methylorubrum extorquens am1 (see paper)
30% identity, 96% coverage: 1:233/243 of query aligns to 2:265/913 of 7vw6A

query
sites
7vw6A

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binding fe2/s2 (inorganic) cluster: H32 (≠ T31), C34 (= C33), H35 (= H34), G45 (= G42), C47 (= C44), R48 (= R45), C50 (= C47), C64 (= C63)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: K263 (= K231)
binding iron/sulfur cluster: C145 (= C124), I146 (= I125), Q147 (≠ L126), C148 (= C127), N149 (≠ G128), C151 (= C130), C155 (= C134), N161 (≠ Y140), V163 (≠ A141), I164 (= I142), V175 (≠ I153), C188 (= C164), V189 (≠ I165), A190 (≠ G166), C191 (= C167), G192 (= G168), C194 (= C170), C198 (= C174), P199 (= P175), T200 (= T176), A202 (= A178), L203 (≠ I179), C227 (= C200), C230 (= C203), C234 (vs. gap), C261 (= C229), K263 (= K231), G264 (≠ C232)

Sites not aligning to the query:

binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 339, 364, 368, 402, 404, 408, 431, 432, 433, 447, 450, 452, 525, 526, 527, 530, 531, 563, 564, 630, 632, 636, 656, 657, 658, 805, 807, 807, 808, 809, 811, 812, 813, 813, 814, 817, 879, 887, 903, 904
binding iron/sulfur cluster: 411

7p8na Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
34% identity, 75% coverage: 1:182/243 of query aligns to 1:204/642 of 7p8na

query
sites
7p8na

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binding fe2/s2 (inorganic) cluster: N32 (≠ T31), C34 (= C33), Y42 (≠ Q41), G43 (= G42), C45 (= C44), R46 (= R45), C48 (= C47), C60 (= C63)
binding iron/sulfur cluster: H92 (vs. gap), D95 (vs. gap), C96 (vs. gap), C99 (vs. gap), C105 (vs. gap), Q108 (vs. gap), C143 (= C124), I144 (= I125), L145 (= L126), C146 (= C127), G147 (= G128), D148 (≠ L129), C149 (= C130), C153 (= C134), V161 (≠ A141), C186 (= C164), V187 (≠ I165), L188 (≠ G166), C189 (= C167), G190 (= G168), C192 (= C170), C196 (= C174), P197 (= P175), T198 (= T176), A200 (= A178), L201 (≠ I179)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 575, 577, 579, 580, 612, 613, 615, 616
binding iron/sulfur cluster: 295, 350, 480, 481, 482, 486

7q5yA Structure of nadh:ubichinon oxidoreductase (complex i) of the hyperthermophilic eubacterium aquifex aeolicus
32% identity, 84% coverage: 1:203/243 of query aligns to 2:232/626 of 7q5yA

query
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7q5yA

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D

K

F

G

L

E

E

-

Y

-

S

-

N

K

R

C
|
C

I
x
V

L
x
V

C
|
C

G
x
Y

L

R

C
|
C

V

T

R

R

A

A

C
|
C

-

D

E

E

A

V

V

V

G

G

V
x
T

Y

R

A

A

I

L

S

Y

S

V

V

E

N

D

R

R

G

G

I

F

T

H

K

S

K

N

I

I

S

V

P

P

P

A

F

V

E

R

E

-

P

-

P

P

E

M

D

D

-

T

-

S

-

T

C
|
C

I

E

G
x
M

C
|
C

G
|
G

A

I

C
|
C

A

V

Q

H

V

V

C
|
C

P
|
P

T

V

G

G

A
|
A

I
|
I

A

I

L

S

K

K

E

P

F

F

E

K

G

-

K

-

R

-

V

-

I

Y

W

W

D

S

K

R

T

S

F

W

E

L

L

L

V

E

K

K

-

G

-

R

-

T

-

V

C
|
C

S

N

V
x
L

C
|
C

binding fe2/s2 (inorganic) cluster: C34 (= C33), Y35 (≠ H34), G43 (= G42), C45 (= C44), R46 (= R45), C48 (= C47), C62 (= C63)
binding iron/sulfur cluster: H96 (≠ A95), D99 (vs. gap), C100 (vs. gap), C103 (vs. gap), A106 (vs. gap), C109 (vs. gap), Q112 (vs. gap), C151 (= C124), V152 (≠ I125), V153 (≠ L126), C154 (= C127), Y155 (≠ G128), C157 (= C130), C161 (= C134), T167 (≠ V139), C193 (= C164), M195 (≠ G166), C196 (= C167), G197 (= G168), C199 (= C170), C203 (= C174), P204 (= P175), A207 (= A178), I208 (= I179), C229 (= C200), L231 (≠ V202), C232 (= C203)

Sites not aligning to the query:

binding iron/sulfur cluster: 234, 236, 264, 265, 382

7qsoG Bovine complex i in lipid nanodisc, state 3 (slack) (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 5:264/700 of 7qsoG

query
sites
7qsoG

M

I

K

E

I

V

K

F

I

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D

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G

L

Q

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C

V

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M

A

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N

E

Y

P

G

G

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Y

T

I

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L

L

E

Q

V

A

A

C

K

E

R

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Q

I

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x
R

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C
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C

H
x
Y

T

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A

R

L

L

P

S

G

V

Q

A

G
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G

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C

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R

L

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C
|
C

I

L

V

V

E

E

V

I

I

E

E

K

G

A

G

-

K

-

N

P

K

K

V

V

V

A

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A

C
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C

L

A

Y

M

P

P

V

V

T

M

N

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E

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I

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E

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I

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L

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T

N

N

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S

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E

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K

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M

A

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N

G

I

V

I

M

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E

L

F

L

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A

L

A

A

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A
x
H

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P

-

L

-
x
D

-
x
C

-

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-

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-
x
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-
x
Q

-
x
G

-

G

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x
C

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-

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x
Q

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Q

H

S

I

M

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M

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D

E

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F

P

L

E

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E

G

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A

N

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E

D

D

K

K

G

N

-

I

-

G

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P

-

L

-

V

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K

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-

I

-

M

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T

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C
|
C

I
|
I

L
x
Q

C
|
C

G
x
T

L
x
R

C
|
C

V

I

R

R

-

F

A

A

C

S

E

E

A

I

V

A

G

G

V

V

Y

D

A

D

I

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T

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T

N

G

R

R

G

G

I

N

T

D

K

M

K

Q

I

V

S

G

P

T

P

Y

F

I

E

E

E

K

P

M

P

F

E

M

D

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C

E

I

L

G

-

C

S

G

G

A

N

C

I

A

I

Q

D

V

I

C
|
C

P
|
P

T
x
V

G
|
G

A
|
A

I

L

A

T

L

S

K

K

E

P

F

Y

E

A

G

F

K

T

R

A

V

R

I

P

W

W

D

E

-

T

-

R

K

K

T

T

F

E

E

S

L

I

V

D

K

V

C

M

S

D

V

A

C

V

G

G

N

S

Y

N

F

I

T

V

-

V

-

S

-

T

-

R

T

T

R

G

E

E

Q

V

Y

M

E

R

F

I

L

L

K

P

Q

R

K

M

Y

H

G

E

L

D

E

I

A

N

E

E

F

W

L

I

C

S

D

D

K

K

C

T

R

R

binding fe2/s2 (inorganic) cluster: R35 (≠ T31), C37 (= C33), Y38 (≠ H34), G46 (= G42), C48 (= C44), R49 (= R45), C51 (= C47), C65 (= C63)
binding iron/sulfur cluster: H97 (≠ A95), D100 (vs. gap), C101 (vs. gap), C104 (vs. gap), Q106 (vs. gap), G107 (vs. gap), C110 (vs. gap), Q113 (≠ E97), C149 (= C124), I150 (= I125), Q151 (≠ L126), C152 (= C127), T153 (≠ G128), R154 (≠ L129), C155 (= C130), C199 (= C174), P200 (= P175), V201 (≠ T176), G202 (= G177), A203 (= A178)

Q91VD9 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; Complex I-75kD; CI-75kD; EC 7.1.1.2 from Mus musculus (Mouse) (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 32:291/727 of Q91VD9

query
sites
Q91VD9

M

I

K

E

I

V

K

F

I

V

D

D

G

G

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A

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G

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C

H

Y

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H

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A

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A

G

G

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C

R

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C
|
C

I

L

V

V

E

E

V

I

I

E

E

K

G

A

G

-

K

-

N

P

K

K

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V

V

A

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A

C
|
C

L

A

Y

M

P

P

V

V

T

M

N

K

E

G

I

W

E

N

V

I

L

L

T

T

N

N

S

S

D

E

K

K

I

S

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K

M

A

R

R

K

E

N

G

I

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I

M

R

E

L

F

L

L

A

L

A

A

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N

A
x
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-

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x
C

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P

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x
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x
C

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-

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E

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E

Q

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P

L

E

E

E

G

K

K

R

R

F

A

N

V

V

E

D

D

K

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N

-

I

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G

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P

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C

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C
|
C

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T

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|
C

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I

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R

-

F

A

A

C

S

E

E

A

I

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A

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G

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Y

D

A

D

I

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S

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V

T

N

G

R

R

G

G

I

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T

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Q

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G

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T

P

Y

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I

E

E

E

K

P

M

P

F

E

M

D

S

C

E

I

L

G

-

C

S

G

G

A

N

C

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A

I

Q

D

V

I

C
|
C

P

P

T

V

G

G

A

A

I

L

A

T

L

S

K

K

E

P

F

Y

E

A

G

F

K

T

R

A

V

R

I

P

W

W

D

E

-

T

-

R

K

K

T

T

F

E

E

S

L

I

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D

K

V

C

M

S

D

V

A

C

V

G

G

N

S

Y

N

F

I

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V

-

V

-

S

-

T

-

R

T

T

R

G

E

E

Q

V

Y

M

E

R

F

I

L

L

K

P

Q

R

K

M

Y

H

G

E

L

D

E

I

A

N

E

E

F

W

L

I

C

S

D

D

K

K

C

T

R

R

C64 (= C33) binding [2Fe-2S] cluster
C75 (= C44) binding [2Fe-2S] cluster
C78 (= C47) binding [2Fe-2S] cluster
C92 (= C63) binding [2Fe-2S] cluster
H124 (≠ A95) binding [4Fe-4S] cluster
C128 (vs. gap) binding [4Fe-4S] cluster
C131 (vs. gap) binding [4Fe-4S] cluster
C137 (vs. gap) binding [4Fe-4S] cluster
C176 (= C124) binding [4Fe-4S] cluster
C179 (= C127) binding [4Fe-4S] cluster
C182 (= C130) binding [4Fe-4S] cluster
C226 (= C174) binding [4Fe-4S] cluster

7v2cM Active state complex i from q10 dataset (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 4:263/690 of 7v2cM

query
sites
7v2cM

M

I

K

E

I

V

K

F

I

V

D

D

G

G

L

Q

E

S

C

V

E

M

A

V

N

E

Y

P

G

G

E

T

Y

T

I

V

L

L

E

Q

V

A

A

C

K

E

R

K

N

V

G

G

I

M

H

Q

I

I

P

P

T

R

L

F

C
|
C

H
x
Y

T

H

D

E

A

R

L

L

P

S

G

V

Q

A

G
|
G

S

N

C
|
C

R
|
R

L

M

C
|
C

I

L

V

V

E

E

V

I

I

E

E

K

G

A

G

-

K

-

N

P

K

K

V

V

V

A

S

A

C
|
C

L

A

Y

M

P

P

V

V

T

M

N

K

E

G

I

W

E

N

V

I

L

L

T

T

N

N

S

S

D

E

K

K

I

S

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K

M

A

R

R

K

E

N

G

I

V

I

M

R

E

L

F

L

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A

L

A

A

R

N

A
x
H

P

P

-

L

-
x
D

-
x
C

-

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I

-
x
C

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D

-
x
Q

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G

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x
C

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-

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E
x
Q

S

D

E

Q

H

S

I

M

R

M

K

F

L

G

K

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E

D

E

R

Y

S

K

R

V

F

P

L

E

E

E

G

K

K

R

R

F

A

N

V

V

E

D

D

K

K

G

N

-

I

-

G

-

P

-

L

-

V

-

K

-

T

-

I

-

M

E

T

K

R

C
|
C

I

I

L

Q

C
|
C

G
x
T

L
x
R

C
|
C

V

I

R

R

-

F

A

A

C

S

E

E

A

I

V

A

G

G

V

V

Y

D

A

D

I

L

S

G

S

T

V

T

N

G

R

R

G

G

I

N

T

D

K

M

K

Q

I

V

S

G

P

T

P

Y

F

I

E

E

E

K

P

M

P

F

E

M

D

S

C

E

I

L

G

-

C

S

G

G

A

N

C

I

A

I

Q

D

V

I

C
|
C

P

P

T
x
V

G

G

A
|
A

I
x
L

A

T

L

S

K

K

E

P

F

Y

E

A

G

F

K

T

R

A

V

R

I

P

W

W

D

E

-

T

-

R

K

K

T

T

F

E

E

S

L

I

V

D

K

V

C

M

S

D

V

A

C

V

G

G

N

S

Y

N

F

I

T

V

-

V

-

S

-

T

-

R

T

T

R

G

E

E

Q

V

Y

M

E

R

F

I

L

L

K

P

Q

R

K

M

Y

H

G

E

L

D

E

I

A

N

E

E

F

W

L

I

C

S

D

D

K

K

C

T

R

R

binding fe2/s2 (inorganic) cluster: C36 (= C33), Y37 (≠ H34), G45 (= G42), C47 (= C44), R48 (= R45), C50 (= C47), C64 (= C63)
binding magnesium ion: Q105 (vs. gap), C198 (= C174), V200 (≠ T176)
binding iron/sulfur cluster: H96 (≠ A95), D99 (vs. gap), C100 (vs. gap), C103 (vs. gap), Q105 (vs. gap), C109 (vs. gap), Q112 (≠ E97), C148 (= C124), C151 (= C127), T152 (≠ G128), R153 (≠ L129), C154 (= C130), C198 (= C174), V200 (≠ T176), A202 (= A178), L203 (≠ I179)

5gupG structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 3:262/673 of 5gupG

query
sites
5gupG

M

I

K

E

I

V

K

F

I

V

D

D

G

G

L

Q

E

S

C

V

E

M

A

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G

G

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Y

T

I

V

L

L

E

Q

V

A

A

C

K

E

R

K

N

V

G

G

I

M

H

Q

I

I

P

P

T

R

L

F

C
|
C

H
x
Y

T

H

D

E

A

R

L

L

P

S

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A

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x
N

C
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C

R

R

L

M

C
|
C

I

L

V

V

E

E

V

I

I

E

E

K

G

A

G

-

K

-

N

P

K

K

V

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V

A

S

A

C
|
C

L

A

Y

M

P

P

V

V

T

M

N

K

E

G

I

W

E

N

V

I

L

L

T

T

N

N

S

S

D

E

K

K

I

S

R

K

M

A

R

R

K

E

N

G

I

V

I

M

R

E

L

F

L

L

A

L

A

A

R

N

A
x
H

P

P

-

L

-

D

-
x
C

-

P

-

I

-
x
C

-

D

-
x
Q

-

G

-

E

-
x
C

-

D

-

L

E
x
Q

S

D

E

Q

H

S

I

M

R

M

K

F

L

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E

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K

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V

F

P

L

E

E

E

G

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D

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K

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N

-

I

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I

-

M

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x
R

C
|
C

I
|
I

L

Q

C
|
C

G
x
T

L

R

C
|
C

V

I

R

R

-

F

A

A

C

S

E

E

A

I

V

A

G

G

V

V

Y

D

A

D

I

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S

G

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V

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N

G

R

R

G

G

I

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x
V

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T

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F

I

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E

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K

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M

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F

E

M

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E

I

L

G

-

C

S

G

G

A

N

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I

A

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Q

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C
|
C

P
|
P

T

V

G

G

A
|
A

I
x
L

A

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S

K

K

E

P

F

Y

E

A

G

F

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V

C

M

S

D

V

A

C

V

G

G

N

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N

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-

V

-

S

-

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-

R

T

T

R

G

E

E

Q

V

Y

M

E

R

F

I

L

L

K

P

Q

R

K

M

Y

H

G

E

L

D

E

I

A

N

E

E

F

W

L

I

C

S

D

D

K

K

C

T

R

R

binding fe2/s2 (inorganic) cluster: C35 (= C33), Y36 (≠ H34), N45 (≠ S43), C46 (= C44), C49 (= C47), C63 (= C63)
binding iron/sulfur cluster: H95 (≠ A95), C99 (vs. gap), C102 (vs. gap), Q104 (vs. gap), C108 (vs. gap), Q111 (≠ E97), R146 (≠ K123), C147 (= C124), I148 (= I125), C150 (= C127), T151 (≠ G128), C153 (= C130), V177 (≠ I153), C197 (= C174), P198 (= P175), A201 (= A178), L202 (≠ I179)

Sites not aligning to the query:

binding : 269, 270, 271, 272, 279, 284, 285, 287

5lc5G Structure of mammalian respiratory complex i, class2 (see paper)
25% identity, 74% coverage: 1:180/243 of query aligns to 2:202/685 of 5lc5G

query
sites
5lc5G

M

I

K

E

I

V

K

F

I

V

D

D

G

G

L

Q

E

S

C

V

E

M

A

V

N

E

Y

P

G

G

E

T

Y

T

I

V

L

L

E

Q

V

A

A

C

K

E

R

K

N

V

G

G

I

M

H

Q

I

I

P

P

T
x
R

L

F

C
|
C

H

Y

T

H

D

E

A

R

L

L

P

S

G

V

Q

A

G
|
G

S

N

C
|
C

R

R

L

M

C
|
C

I

L

V

V

E

E

V

I

I

E

E

K

G

A

G

-

K

-

N

P

K

K

V

V

V

A

S

A

C
|
C

L

A

Y

M

P

P

V

V

T

M

N

K

E

G

I

W

E

N

V

I

L

L

T

T

N

N

S

S

D

E

K

K

I

T

R

K

M

A

R

R

K

E

N

G

I

V

I

M

R

E

L

F

L

L

A

L

A

A

R

N

A
x
H

P

P

-

L

-
x
D

-
x
C

-

P

-

I

-
x
C

-

D

-

Q

-

G

-

E

-
x
C

-

D

-

L

E
x
Q

S

D

E

Q

H

S

I

M

R

M

K

F

L

G

K

S

E

D

E

R

Y

S

K

R

V

F

P

L

E

E

E

G

K

K

R

R

F

A

N

V

V

E

D

D

K

K

G

N

-

I

-

G

-

P

-

L

-

V

-

K

-

T

-

I

-

M

E

T

K

R

C
|
C

I
|
I

L

Q

C
|
C

G

T

L

R

C
|
C

V

I

R

R

-

F

A

A

C

S

E

E

A

I

V

A

G

G

V

V

Y

D

A

D

I

L

S

G

S

T

V

T

N

G

R

R

G

G

I

N

T

D

K

M

K

Q

I

V

S

G

P

T

P

Y

F

I

E

E

E

K

P

M

P

F

E

M

D

S

C

E

I

L

G

S

C

-

G

G

A

N

C

I

A

I

Q

D

V

I

C
|
C

P

P

T

V

G
|
G

A

A

I

L

A

T

binding fe2/s2 (inorganic) cluster: R32 (≠ T31), C34 (= C33), G43 (= G42), C45 (= C44), C48 (= C47), C62 (= C63)
binding iron/sulfur cluster: H94 (≠ A95), D97 (vs. gap), C98 (vs. gap), C101 (vs. gap), C107 (vs. gap), Q110 (≠ E97), C146 (= C124), I147 (= I125), C149 (= C127), C152 (= C130), C196 (= C174), G199 (= G177)

5xf9B Crystal structure of NAD+-reducing [nife]-hydrogenase in the air- oxidized state (see paper)
26% identity, 75% coverage: 15:196/243 of query aligns to 17:220/234 of 5xf9B

query
sites
5xf9B

G

G

E

Q

Y

T

I

V

L

L

E

E

V

A

A

A

K

L

R

A

N

A

G

G

I

R

H

Y

I

I

P

P

T

H

L

L

C
|
C

H

W

T

H

D

P

A

E

L

M

P

G

G

N

Q

H

G
|
G

S

S

C
|
C

R
|
R

L

L

C
|
C

I

V

V

V

E

E

V

A

I

-

E

-

G

-

K

N

N

G

K

R

V

I

V

Q

V

A

S

S

C
|
C

L

A

Y

L

P

P

V

A

T

Q

N

P

E

G

I

L

E

Q

V

V

L

V

T

S

N

K

S

S

D

E

K

T

I

L

R

T

R

R

M

V

R

R

K

R

N

T

I

L

R

E

L

M

L

L

A
x
F

A

A

R

E

A

G

-

N

-
x
H

-
x
F

-
x
C

P

P

E

G

S
x
C

E

E

-

K

-
x
S

-

G

-

D

-
x
C

-

L

-

Q

-

A

-

L

-

A

H

Y

I

A

R

H

K

G

L

M

K

T

E

A

E

S

Y

H

K

F

V

D

P

P

E

F

E

Y

K

P

R

Q

F

R

N

R

V

I

D

D

K

A

G

S

E

H

-

P

-

D

-

L

-

W

-

L

-

D

-

P

-

N

K

R

C
|
C

I
|
I

L
|
L

C
|
C

G
|
G

L

L

C
|
C

V

V

R

R

A

A

C

S

E

L

A

A

V

E

G

G

V

K

Y

E

A

A

I

L

S

V

S

I

V

G

N

G

R

R

G

G

I

I

T

A

K

S

K

R

I
x
L

-

L

-

A

-

T

S

S

P

A

P

S

F

G

E

R

E

L

P

G

P

D

E

T

D

A

C

L

I

A

G

A

C

T

G

D

A

R

C

A

A

A

Q

R

V

I

C
|
C

P

P

T

V

G
|
G

A
|
A

I

L

A

N

L

F

K

K

E

A

F

A

E

G

G

F

K

T

R

T

V

P

I

I

W

G

D

K

K

R

T

R

F

F

E

D

binding fe2/s2 (inorganic) cluster: C35 (= C33), G44 (= G42), C46 (= C44), R47 (= R45), C49 (= C47), C61 (= C63)
binding iron/sulfur cluster: F90 (≠ A92), H95 (vs. gap), F96 (vs. gap), C97 (vs. gap), C100 (≠ S98), S103 (vs. gap), C106 (vs. gap), C145 (= C124), I146 (= I125), L147 (= L126), C148 (= C127), G149 (= G128), C151 (= C130), L174 (≠ I153), C198 (= C174), G201 (= G177), A202 (= A178)

7b0nG 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
24% identity, 79% coverage: 1:192/243 of query aligns to 3:217/694 of 7b0nG

query
sites
7b0nG

M

I

K

E

I

L

K

T

I

I

D

D

G

G

L

H

E

K

C

V

E

S

A

I

N

E

Y

A

G

G

E

S

Y

A

I

L

L

I

E

Q

V

A

A

C

K

E

R

K

N

A

G

G

I

V

H

T

I

V

P

P

T

R

L

Y

C
|
C

H

Y

T

H

D

D

A

K

L

L

P

A

G

I

Q

A

G
|
G

S

N

C
|
C

R
|
R

L

M

C
|
C

I

L

V

V

E

D

V

V

I

E

E

R

G

A

G

-

K

-

N

P

K

K

V

P

V

V

V

A

S

S

C
|
C

L

A

Y

Y

P

P

V

V

T

A

N

P

E

G

I

M

E

V

V

V

L

R

T

T

N

D

S

T

D

E

K

R

I

V

R

K

R

Q

M

A

R

R

K

E

N

N

I

V

I

M

R

E

L

M

A

L

A

Q

R

N

A
x
H

P

P

-

L

-

D

-
x
C

-

P

-

V

-
x
C

-

D

-

Q

-

G

-

E

-
x
C

-

D

-

L

E
x
Q

S

D

E

Q

H

S

I

M

R

R

K

Y

L

G

K

R

E

D

E

R

Y

G

K

R

V

F

P

T

E

E

-

I

-

T

E

G

K

K

R

R

F

S

N

T

V

E

D

D

K

K

G

N

-

I

-

G

-

P

-

L

-

V

-

K

-

T

-

S

-

M

E

N

K

R

C
|
C

I

I

L
x
H

C
|
C

G
x
T

L

R

C
|
C

V

V

R

R

-

F

A

A

C

N

E

D

A

I

V

A

G

G

V

A

Y

P

A

E

I

L

S

G

S

S

V

S

N

G

R

R

G

G

I

N

T

D

K

M

K

Q

I

I

S

G

P

T

P

Y

F

L

E

E

E

K

P

N

P

L

E

N

D

T

C

E

I

L

G

-

C

S

G

G

A

N

C

V

A

I

Q

D

V

L

C
|
C

P

P

T
x
V

G

G

A

A

I

L

A

T

L

N

K

K

E

P

F

Y

E

A

G

F

K

R

R

A

V

R

I

P

W

W

D

E

binding fe2/s2 (inorganic) cluster: C35 (= C33), G44 (= G42), C46 (= C44), R47 (= R45), C49 (= C47), C63 (= C63)
binding iron/sulfur cluster: H95 (≠ A95), C99 (vs. gap), C102 (vs. gap), C108 (vs. gap), Q111 (≠ E97), C149 (= C124), H151 (≠ L126), C152 (= C127), T153 (≠ G128), C155 (= C130), C199 (= C174), V201 (≠ T176)

8oh5C Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
44% identity, 34% coverage: 122:204/243 of query aligns to 611:696/1172 of 8oh5C

query
sites
8oh5C

E

D

K

K

C
|
C

I
x
V

L
|
L

C
|
C

G
|
G

L

L

C
|
C

V

V

R

R

A

V

C
|
C

-

D

E

E

A

V

V

V

G

G

V

A

Y

T

A

A

I

I

S

G

S

L

V

V

N

G

R

R

G

G

I

F

T

D

K

S

K

V

I
|
I

S

M

P

P

P

E

F

F

E

K

E

L

P

P

P

L

E

S

D

E

-

T

-

A

C
|
C

I
|
I

G
x
S

C
|
C

G
|
G

A

Q

C
|
C

A

V

Q

D

V

V

C
|
C

P

P

T

T

G
|
G

A
|
A

I

C

A

M

L

E

K

K

E

Q

F

V

E

S

G

Y

K

K

R

Q

V

I

I

P

W

A

D

N

K

M

T

D

F

S

E

M

L

A

V

S

K

V

C
|
C

S

G

V
x
Y

C
|
C

G

G

binding iron/sulfur cluster: C613 (= C124), V614 (≠ I125), L615 (= L126), C616 (= C127), G617 (= G128), C619 (= C130), C623 (= C134), I643 (= I153), C656 (= C164), I657 (= I165), S658 (≠ G166), C659 (= C167), G660 (= G168), C662 (= C170), C666 (= C174), G669 (= G177), A670 (= A178), C692 (= C200), Y694 (≠ V202), C695 (= C203)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 199, 201, 202, 203, 222, 223, 229, 230, 235, 239, 264, 265, 285, 288, 332, 471, 477, 479
binding fe2/s2 (inorganic) cluster: 36, 45, 47, 48, 50, 64
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 330, 331, 332, 333, 353, 354, 355, 432, 433, 434, 477
binding iron/sulfur cluster: 96, 98, 100, 102, 104, 108, 147, 149, 151, 155, 156, 164, 170, 480, 562, 563, 565, 567, 573, 699, 725, 727, 728, 853

7nyrG Respiratory complex i from escherichia coli - conformation 1 (see paper)
25% identity, 95% coverage: 3:233/243 of query aligns to 4:267/907 of 7nyrG

query
sites
7nyrG

I

I

K

H

I

V

D

D

G

G

L

K

E

E

C

Y

E

E

A

V

N

N

Y

G

G

A

E

D

Y

N

I

L

L

L

E

E

V

A

A

C

K

L

R

S

N

L

G

G

I

L

H

D

I

I

P

P

T

Y

L

F

C
|
C

H
x
W

T

H

D

P

A

A

L

L

P

G

G

S

Q

V

G

G

S

A

C
|
C

R
|
R

L

Q

C
|
C

I

A

V

V

-

K

-

Q

-

Y

E

Q

V

N

I

A

E

E

G

D

G

T

K

R

N

G

K

R

V

L

V

V

V
x
M

S

S

C
|
C

L

M

Y

T

P

P

V

A

T

S

N

D

E

G

I

T

E

F

V

I

L

S

T

I

N

D

S

D

D

E

K

E

I

A

R

K

R

Q

M

F

R

R

K

E

N

S

I

V

R

E

L

W

L

L

A

M

A

T

R

N

A
x
H

P

P

E

H

-
x
D

-
x
C

-

P

-

V

-
x
C

-

E

-
x
G

-

G

S

N

E
x
C

H

H

I

L

R
x
Q

K

D

L

M

K

T

-

V

-

M

-

T

-

G

-

H

-

S

-

F

E

R

Y

Y

K

R

V

F

P

T

E

K

E

R

K

T

R

H

F

R

N

N

V

Q

D

D

K

L

G

G

-

P

-

F

-

I

-

S

-

H

-

E

-

M

E

N

K

R

C
|
C

I
|
I

L
x
A

C
|
C

G
x
Y

L

R

C
|
C

V

V

R

R

A

Y

C

Y

E

K

A

D

V

-

G

-

V

-

Y

Y

A

A

I

-

S

D

S

G

V

T

N

D

R

L

G

G

I

V

T

Y

K

G

K

A

I

H

S

D

P

N

P

V

F

Y

E
x
F

E

G

P

R

P

P

E

E

D

D

C

G

I

T

-

L

-

E

-

S

-

E

G

F

C

S

G

G

A

N

C

L

A

V

Q

E

V

I

C
|
C

P

P

T

T

G
|
G

A
x
V

I
x
F

A

T

L

D

K

K

E

T

F

H

-

S

E

E

G

R

K

Y

R

N

V

R

I

K

W

W

D

D

K

M

T

Q

F

F

E

A

L

P

V

S

K

I

C
|
C

S

Q

V

Q

C
|
C

-

S

-
x
I

G

G

N
x
C

Y

N

F

I

T

S

T

P

R

G

E

E

Q

R

Y

Y

E

G

F

E

L

L

K

R

Q

R

-

I

-

E

-

N

K

R

Y

Y

-

N

G

G

L

T

E

V

A

N

E

H

E

Y

F

F

L

L

C
|
C

D

D

K

R

C
x
G

R

R

binding fe2/s2 (inorganic) cluster: C34 (= C33), W35 (≠ H34), C45 (= C44), R46 (= R45), C48 (= C47), M65 (≠ V61), C67 (= C63)
binding iron/sulfur cluster: H99 (≠ A95), D102 (vs. gap), C103 (vs. gap), C106 (vs. gap), G109 (vs. gap), C112 (≠ E99), Q115 (≠ R102), C151 (= C124), I152 (= I125), A153 (≠ L126), C154 (= C127), Y155 (≠ G128), C157 (= C130), F181 (≠ E158), C201 (= C174), G204 (= G177), V205 (≠ A178), F206 (≠ I179), C228 (= C200), C231 (= C203), I233 (vs. gap), C235 (≠ N205), C263 (= C229), G266 (≠ C232)

Sites not aligning to the query:

binding calcium ion: 617, 632, 647, 731

7awtG E. Coli nadh quinone oxidoreductase hydrophilic arm (see paper)
25% identity, 95% coverage: 3:233/243 of query aligns to 3:266/907 of 7awtG

query
sites
7awtG

I

I

K

H

I

V

D

D

G

G

L

K

E

E

C

Y

E

E

A

V

N

N

Y

G

G

A

E

D

Y

N

I

L

L

L

E

E

V

A

A

C

K

L

R

S

N

L

G

G

I

L

H

D

I

I

P

P

T
x
Y

L

F

C
|
C

H
x
W

T

H

D

P

A

A

L

L

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G

G

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G

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R

L

Q

C
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C

I

A

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V

-

K

-

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-

Y

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A

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E

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C

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A
x
H

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-
x
D

-
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C

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P

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x
C

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-

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C

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x
Q

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Y

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N

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x
Y

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C

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V

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R

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Y

E

K

A

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G

-

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-

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Y

A

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x
F

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x
V

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C

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T

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G

A
x
V

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F

A

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N

V

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A

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C

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x
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C

-

S

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x
I

G

G

N
x
C

Y

N

F

I

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T

P

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E

Q

R

Y

Y

E

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F

E

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I

-

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K

R

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Y

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N

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G

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C

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binding fe2/s2 (inorganic) cluster: Y31 (≠ T31), C33 (= C33), W34 (≠ H34), G42 (= G42), C44 (= C44), R45 (= R45), C47 (= C47), C66 (= C63)
binding magnesium ion: V197 (≠ A171), C200 (= C174), T202 (= T176)
binding iron/sulfur cluster: H98 (≠ A95), D101 (vs. gap), C102 (vs. gap), C105 (vs. gap), C111 (≠ E99), Q114 (≠ R102), C150 (= C124), I151 (= I125), A152 (≠ L126), C153 (= C127), Y154 (≠ G128), C156 (= C130), F180 (≠ E158), C200 (= C174), P201 (= P175), T202 (= T176), T202 (= T176), G203 (= G177), V204 (≠ A178), C227 (= C200), Q229 (≠ V202), C230 (= C203), I232 (vs. gap), C234 (≠ N205), C262 (= C229)

Sites not aligning to the query:

8bedG Cryo-em structure of the arabidopsis thaliana i+iii2 supercomplex (ci peripheral tip) (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 18:277/687 of 8bedG

query
sites
8bedG

M

I

K

E

I

V

K

F

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D

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G

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C

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C

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E

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G

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C

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A

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F

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binding fe2/s2 (inorganic) cluster: R48 (≠ T31), C50 (= C33), Y51 (≠ H34), G59 (= G42), C61 (= C44), R62 (= R45), C64 (= C47), C78 (= C63)
binding iron/sulfur cluster: H110 (≠ A95), D113 (vs. gap), C114 (vs. gap), C117 (vs. gap), Q119 (vs. gap), G120 (vs. gap), C123 (vs. gap), L125 (vs. gap), Q126 (≠ E97), C162 (= C124), I163 (= I125), Q164 (≠ L126), C165 (= C127), T166 (≠ G128), C168 (= C130), I192 (= I153), C212 (= C174), V214 (≠ T176), A216 (= A178), L217 (≠ I179)
binding : I116 (vs. gap), F224 (≠ G186)

Sites not aligning to the query:

binding : 305, 306, 589, 590, 595, 596, 599, 600, 602, 603, 606

7a23C Plant mitochondrial respiratory complex i (see paper)
25% identity, 96% coverage: 1:233/243 of query aligns to 25:284/693 of 7a23C

query
sites
7a23C

M

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x
V

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G

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A

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K

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F

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A

G

F

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R

A

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N

W

W

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-

L

K

K

T

A

F

T

E

E

L

T

V

I

K

D

C

V

S

S

-

D

-

A

V

V

C

G

G

S

N

N

Y

I

F

R

T

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T

D

R

S

E

R

Q

G

Y

P

E

E

F

V

L

M

K

R

Q

I

K

I

Y

P

G

R

L

L

E

N

-

E

-

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-

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A

N

E

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C

S

D

D

K

K

C

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R

R

binding fe2/s2 (inorganic) cluster: R55 (≠ T31), C57 (= C33), Y58 (≠ H34), C68 (= C44), C71 (= C47), C85 (= C63)
binding iron/sulfur cluster: H117 (≠ A95), D120 (vs. gap), C121 (vs. gap), C124 (vs. gap), G127 (vs. gap), C130 (vs. gap), L132 (vs. gap), Q133 (≠ E97), C169 (= C124), I170 (= I125), Q171 (≠ L126), C172 (= C127), T173 (≠ G128), R174 (≠ L129), C175 (= C130), I199 (= I153), C219 (= C174), P220 (= P175), V221 (≠ T176), G222 (= G177)

P29915 NADH-quinone oxidoreductase chain 3; NADH dehydrogenase I, chain 3; NDH-1, chain 3; EC 7.1.1.- from Paracoccus denitrificans (see 2 papers)
24% identity, 95% coverage: 2:233/243 of query aligns to 6:273/673 of P29915

query
sites
P29915

K

K

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D

D

G

D

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E

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D

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R

H106 (≠ A95) mutation to A: Very little incorporation of iron-sulfur centers into protein in E.coli.; mutation to C: Alters the EPR signal of the N5 cluster; all 3 iron-sulfur clusters are less stable in E.coli.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_068747956.1 NCBI__GCF_001601575.1:WP_068747956.1
MKIKIDGLECEANYGEYILEVAKRNGIHIPTLCHTDALPGQGSCRLCIVEVIEGGKNKVV
VSCLYPVTNEIEVLTNSDKIRRMRKNIIRLLAARAPESEHIRKLKEEYKVPEEKRFNVDK
GEKCILCGLCVRACEAVGVYAISSVNRGITKKISPPFEEPPEDCIGCGACAQVCPTGAIA
LKEFEGKRVIWDKTFELVKCSVCGNYFTTREQYEFLKQKYGLEAEEFLCDKCRKKSTAQK
LVF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory