SitesBLAST

5exdH Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
30% identity, 81% coverage: 6:161/192 of query aligns to 10:167/291 of 5exdH

query
sites
5exdH

E

Q

I

I

R

T

W

V

H

W

A

A

R

R

G

G

-

V

-

M

G

N

Q

K

G

D

A

A

K

R

T

D

A

I

A

V

L

V

M

A

L

L

A

T

E

E

S

A

V

A

A

A

E

K

M

E

G

G

K

K

Y

Y

V

V

Q

Q

G

A

F

W

P

E

E

N

Y

Y

G

V

-

D

-

L

P

P

E

D

R

R

M

I

G

Y

A

V

P

P

I

V

L

R

A

A

F

Y

N

A

R

R

I

I

S

S

D

S

E

D

P

P

I

I

-

E

-

S

-

K

F

Y

V

I

H

Y

S

E

N

N

V

E

T

T

N

-

P

P

N

D

I

I

V

V

I

V

V

L

L

V

D

E

P

E

T

S

L

L

L

I

G

K

K

G

A

V

K

P

I

I

T

L

D

K

G

G

V

I

P

R

E

P

D

G

G

S

I

T

Y

L

I

V

N

N

T

T

S

K

K

R

T

S

P

I

Q

D

E

T

I

I

R

L

K

E

I

F

L

L

G

G

I

D

T

T

-

G

-

N

T

L

G

A

K

Q

I

I

F

V

T

T

I

V

D

D

A

A

T

N

Q

S

I

M

S

A

I

G

E

I

T

A

I

A

G

P

R

-

P

-

I

-

P

-

N

-

T

-

P

-

M

I

M

A

G

G

A

A

F

V

I

V

R

K

A

A

T

T

G

G

I

I

I

V

P

D

F

V

E

E

T

N

F

L

M

A

E

A

H

V

M

V

K

K

Sites not aligning to the query:

binding iron/sulfur cluster: 230, 237, 238, 239, 240, 241, 243, 247, 259, 266, 267, 268, 269, 270, 272, 276, 278, 281

5c4iE Structure of an oxalate oxidoreductase (see paper)
31% identity, 64% coverage: 6:127/192 of query aligns to 13:143/312 of 5c4iE

query
sites
5c4iE

E

Q

I

I

R

T

W

V

H

W

A

A

R

R

G

G

-

V

-

M

G

N

Q

K

G

D

A

A

K

R

T

D

A

I

A

V

L

V

M

A

L

L

A

T

E

E

S

A

V

A

A

A

E

K

M

E

G

G

K

K

Y

Y

V

V

Q

Q

G

A

F

W

P

E

E

N

Y

Y

G

V

-

D

-

L

P

P

E

D

R

R

M

I

G

Y

A

V

P

P

I

V

L

R

A

A

F

Y

N

A

R

R

I

I

S

S

D

S

E

D

P

P

I

I

-

E

-

S

-

K

F

Y

V

I

H

Y

S

E

N

N

V

E

T

T

N

-

P

P

N

D

I

I

V

V

I

V

V

L

L

V

D

E

P

E

T

S

L

L

L

I

G

K

K

G

A

V

K

P

I

I

T

L

D

K

G

G

V

I

P

R

E

P

D

G

G

S

I

T

Y

L

I

V

N

N

T

T

S

K

K

R

T

S

P

I

Q

D

E

T

I

I

R

L

K

E

I

F

L

L

G

G

I

D

T

T

-

G

-

N

T

L

G

A

K

Q

I

I

F

V

T

T

I

V

D

D

A

A

T

N

Q

S

I

M

S

A

Sites not aligning to the query:

binding iron/sulfur cluster: 258, 259, 260, 261, 262, 264, 268, 272, 287, 288, 289, 290, 291, 293, 297, 298, 299, 302

2c3uA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus, oxygen inhibited form (see paper)
24% identity, 79% coverage: 34:185/192 of query aligns to 447:608/1231 of 2c3uA

query
sites
2c3uA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: 28, 29, 63, 87, 816, 837, 839, 868, 869, 961, 962, 963, 964, 992, 993, 994, 995, 996
binding calcium ion: 982, 984, 1055, 1056, 1058, 1060, 1062
binding magnesium ion: 962, 990, 992
binding pyruvic acid: 30, 113, 122, 995
binding iron/sulfur cluster: 681, 683, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 811, 814, 816, 839, 1070, 1071

2c3pA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
24% identity, 79% coverage: 34:185/192 of query aligns to 447:608/1231 of 2c3pA

query
sites
2c3pA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: 28, 29, 30, 63, 87, 113, 816, 837, 838, 839, 868, 869, 961, 962, 963, 992, 993, 994, 995, 996
binding calcium ion: 982, 983, 984, 1055, 1056, 1058, 1060, 1062
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839, 994, 1070, 1071

2c3oA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
24% identity, 79% coverage: 34:185/192 of query aligns to 447:608/1231 of 2c3oA

query
sites
2c3oA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1056, 1058, 1060
binding magnesium ion: 962, 990, 992
binding pyruvic acid: 30, 113, 996
binding iron/sulfur cluster: 681, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 746, 747, 748, 749, 750, 754, 760, 761, 810, 811, 814, 816, 839, 1070, 1071
binding thiamine diphosphate: 28, 29, 63, 87, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996

1kekA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase (see paper)
24% identity, 79% coverage: 34:185/192 of query aligns to 447:608/1231 of 1kekA

query
sites
1kekA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1055, 1056, 1058, 1062
binding 2-acetyl-thiamine diphosphate: 29, 63, 87, 113, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 703, 737, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839, 1070, 1071

1b0pA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
24% identity, 79% coverage: 34:185/192 of query aligns to 447:608/1231 of 1b0pA

query
sites
1b0pA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1055, 1056, 1058, 1060
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 737, 744, 745, 747, 748, 750, 754, 755, 760, 811, 814, 816, 839, 994, 1070, 1071
binding thiamine diphosphate: 28, 29, 63, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996

P94692 Pyruvate:ferredoxin oxidoreductase; PFOR; POR; Pyruvate synthase; EC 1.2.7.1 from Desulfocurvibacter africanus (Desulfovibrio africanus) (see 3 papers)
24% identity, 79% coverage: 34:185/192 of query aligns to 448:609/1232 of P94692

query
sites
P94692

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R

K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

V

-

G

I

L

G

G

R

G

P

R

I

I

P

-

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

P

F

F

E

E

T

K

F

A

M

V

E

D

H

L

M

L

K

K

E

K

Q

S

L

I

G

H

K

K

K

A

F

Y

K

G

S

K

K

K

S

G

E

E

-

K

V

I

I

V

E

K

G

M

N

N

L

T

K

D

A

A

I

V

E

D

R

Q

A

A

Sites not aligning to the query:

31 binding pyruvate
64 binding thiamine diphosphate
114 binding pyruvate
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
695 binding [4Fe-4S] cluster
699 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
751 binding [4Fe-4S] cluster
755 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
815 binding [4Fe-4S] cluster
817 binding thiamine diphosphate
840 binding [4Fe-4S] cluster; binding thiamine diphosphate
962:965 binding thiamine diphosphate
963 binding Mg(2+)
983 binding Ca(2+)
985 binding Ca(2+)
991 binding Mg(2+)
991:996 binding thiamine diphosphate
993 binding Mg(2+)
1056 binding Ca(2+)
1059 binding Ca(2+)
1061 binding Ca(2+)
1063 binding Ca(2+)
1071 binding [4Fe-4S] cluster

7plmA Cryoem reconstruction of pyruvate ferredoxin oxidoreductase (pfor) in anaerobic conditions (see paper)
22% identity, 63% coverage: 34:154/192 of query aligns to 447:573/1177 of 7plmA

query
sites
7plmA

Y

F

V

A

Q

Q

G

G

F

Y

P

F

E

S

Y

Y

G

D

P

S

E

K

R
x
K

M

S

G

G

A

G

P

I

I

T

L

I

A

S

F

H

N

L

R

R

I

F

S

G

D

E

E

K

P

P

I

I

F

Q

V

S

H

T

S

Y

N

L

V

V

T

N

N

R

P

A

N

D

I

Y

V

V

I

A

V

C

L

H

D

N

P

P

T

A

L

Y

L

V

G

G

K

I

A

Y

K

D

I

I

T

L

D

E

G

G

V

I

P

K

E

D

D

G

G

G

I

T

Y

F

I

V

I

L

N

N

T

S

-

P

-

W

-

S

-

L

-

E

-

D

-

M

-

D

S

K

K

H

T

L

P

P

Q

S

E

G

I

I

R

K

K

R

I

T

L

I

G

A

I

N

T

K

G

L

K

K

I

F

F

Y

T

N

I

I

D

D

A

A

T

V

Q

K

I

I

S

A

I

T

E

D

T

-

I

V

G

G

R

R

P

-

I

-

P

I

N

N

T

M

P

I

M

M

M

Q

G

T

A

A

F

F

I

F

R

K

A

L

T

A

G

G

I

V

I

L

P

A

F

V

E

D

binding iron/sulfur cluster: K458 (≠ R45)

Sites not aligning to the query:

binding calcium ion: 950, 952, 1026, 1028
binding magnesium ion: 930, 958, 960, 962
binding iron/sulfur cluster: 657, 659, 664, 665, 666, 667, 668, 670, 674, 675, 679, 708, 715, 716, 717, 718, 719, 721, 725, 726, 731, 732, 781, 782, 785, 787, 810, 962, 1038, 1039
binding thiamine diphosphate: 27, 28, 29, 63, 787, 808, 809, 810, 839, 929, 930, 931, 960, 961, 962, 963, 964

Query Sequence

>WP_068748417.1 NCBI__GCF_001601575.1:WP_068748417.1
MAKVLEIRWHARGGQGAKTAALMLAESVAEMGKYVQGFPEYGPERMGAPILAFNRISDEP
IFVHSNVTNPNIVIVLDPTLLGKAKITDGVPEDGIYIINTSKTPQEIRKILGITTGKIFT
IDATQISIETIGRPIPNTPMMGAFIRATGIIPFETFMEHMKEQLGKKFKSKSEVIEGNLK
AIERAYMEVKSE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory