SitesBLAST

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
36% identity, 75% coverage: 2:367/488 of query aligns to 4:375/384 of P75063

query
sites
P75063

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I14 (≠ V12) binding FAD
E33 (= E31) binding FAD
TS 42:43 (≠ TT 40:41) binding FAD
SGV 47:49 (≠ SAI 45:47) binding FAD
V177 (= V172) binding FAD
MK 346:347 (≠ IQ 335:336) binding FAD
T352 (≠ S341) binding FAD

3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
29% identity, 72% coverage: 3:354/488 of query aligns to 4:365/366 of 3dmeA

query
sites
3dmeA

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binding flavin-adenine dinucleotide: I8 (= I7), G9 (= G8), G11 (= G10), V12 (= V11), V13 (= V12), E32 (= E31), A33 (≠ K32), T41 (= T40), S42 (≠ T41), R44 (≠ A43), N45 (= N44), S46 (= S45), V48 (≠ I47), H50 (= H49), P170 (= P171), L171 (≠ V172), A203 (= A200), I313 (= I309), R314 (= R310), I346 (= I335), E347 (≠ Q336), S348 (= S337), P349 (= P338), G350 (= G339), L351 (= L340), T352 (≠ S341)

8w7fB Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase bound with fad and a sulfate ion (see paper)
26% identity, 68% coverage: 24:357/488 of query aligns to 27:408/412 of 8w7fB

query
sites
8w7fB

N

S

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L

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E
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A

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x
H

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x
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H

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x
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H

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K

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T

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I

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E

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N

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A

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V

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R

A

A

V

Q

A

A

E

M

-

D

-

L

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D

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G

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N

-

L

T

V

D

D

F

F

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V

I

F

N

D

A

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Q

Q

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G

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S

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L

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P

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x
A

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x
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E

binding flavin-adenine dinucleotide: E34 (= E31), K35 (= K32), H42 (≠ G39), Q43 (≠ T40), S44 (≠ T41), H46 (≠ A43), N47 (= N44), S48 (= S45), V50 (≠ I47), H52 (= H49), V173 (= V172), G205 (≠ A200), G206 (= G201), Q208 (≠ Y203), Y231 (= Y225), V350 (≠ I309), R351 (= R310), P389 (= P338), G390 (= G339), A391 (≠ L340), T392 (≠ S341)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 12, 13

8w78A Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase in complex with fad and 2-oxoglutarate (see paper)
26% identity, 68% coverage: 24:357/488 of query aligns to 27:406/410 of 8w78A

query
sites
8w78A

N

S

L

L

D

K

V

V

V

A

L

V

L

L

E
|
E

K
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K

G

E

D

C

D

K

V

L

A

A

S

K

G
x
H

T
x
Q

T
x
S

K

G

A

H

N
|
N

S
|
S

A

G

I
x
V

I

I

H
|
H

A

A

G

G

Y

I

D

Y

A

Y

K

K

P

P

G

G

T

T

L

L

K

K

A

A

K

R

L

L

N

C

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V

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E

G

G

N

M

F

H

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L

F

A

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Y

K

A

I

Y

C

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E

D

E

E

L

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D

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F

Y

K

K

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K

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T

G

G

S

K

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L

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I

L

V

A

A

F

T

N

D

D

E

E

K

E

E

I

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K

K

E

L

I

L

E

K

N

D

L

L

L

E

E

K

R

R

G

G

K

I

I

A

N

N

G

N

I

V

P

P

Q

D

I

L

E

R

I

M

I

I

G

E

K

G

E

S

D

E

I

I

L

Q

K

E

M

I

E

E

P

P

N

Y

V

C

N

Q

K

G

E

V

V

M

K

-

A

-

A

A

L

L

F

H

A

S

K

P

T

H

A

T

G

G

I

I

I

V

C

D

P

W

Y

G

E

L

L

V

A

T

Q

E

A

H

F

Y

G

G

E

Q

N

D

A

F

F

K

L

Q

N

C

G

G

-

G

-

D

V

I

E

Y

F

L

K

D

F

F

N

N

S

-

P

-

V

-

I

-

G

-

I
x
V

E

S

K

K

L

F

K

T

D

E

G

T

F

D

I

Y

V

P

K

V

T

T

T

I

H

H

-

G

-

A

-

K

-

P

-

G

E

Q

D

T

I

V

H

R

A

T

R

K

F

N

I

V

I

L

N

T

A

C

A
x
G

G
|
G

V

L

Y
x
Q

A

S

D

D

E

L

I

L

A

A

-

E

R

K

M

T

A

G

N

C

A

P

E

R

E

D

Y

P

K

R

I

I

I

V

P

P

R

F

K

R

G

G

E

E

Y

Y

L

L

F

L

D

T

K

K

S

E

V

K

G

Q

N

H

I

M

V

V

N

K

K

G

V

N

L

I

F
x
Y

P

P

T

V

P

P

T

D

K

P

I

R

S

F

K

P

-
x
F

-
x
L

G

G

I

V

L
x
H

V

F

S

T

P

P

T

R

V

M

D

D

G

G

N

S

F

I

F

W

I

L

G

G

P

P

N

N

S

A

N

V

N

L

Q

A

E

L

S

K

K

R

E

E

D

G

T

Y

S

T

-

W

-

G

-

D

-

I

-

N

-

L

-

F

-

E

-

L

-

F

-

D

-

A

-

L

-

R

-

Y

-

P

-

G

-

F

V

V

T

K

L

M

E

A

-

S

-

K

-

Y

-

I

-

G

-

F

G

G

I

L

E

S

E

E

I

M

-

S

-

K

-

S

-

W

-

F

-

I

-

N

-

L

-

Q

I

I

K

K

G

A

A

L

Q

Q

R

K

L

Y

V

I

P

P

N

D

I

I

P

T

L

E

K

Y

S

D

V

I

I

Q

T

R

S

G

F

P

A

A

G

G

I

V

R
|
R

A

A

V

Q

A

A

E

M

-

D

-

L

-

D

-

G

-

N

-

L

T

V

D

D

F

F

V

V

I

F

N

D

A

R

S

G

Q

Q

K

G

-

S

-

G

-

A

-

L

V

A

K

K

G

R

F

V

I

L

N

H

A

C

G

R

G

N

I

A

Q

P

S

S

P
|
P

G
|
G

L
x
A

S
x
T

A

S

P

L

A

A

I

I

A

A

E

K

M

M

V

I

I

A

E

D

I

K

L

I

K

E

D

N

E

E

binding 2-oxoglutaric acid: S48 (= S45), H52 (= H49), Y245 (≠ F241), F254 (vs. gap), L255 (vs. gap), H258 (≠ L252), R349 (= R310)
binding flavin-adenine dinucleotide: E34 (= E31), K35 (= K32), H42 (≠ G39), Q43 (≠ T40), S44 (≠ T41), N47 (= N44), S48 (= S45), V50 (≠ I47), H52 (= H49), V173 (≠ I175), G203 (≠ A200), G204 (= G201), Q206 (≠ Y203), R349 (= R310), P387 (= P338), G388 (= G339), A389 (≠ L340), T390 (≠ S341)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 8, 11, 12, 13

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
29% identity, 68% coverage: 3:335/488 of query aligns to 51:407/866 of Q9UI17

query
sites
Q9UI17

D

E

V

T

V

V

I

I

G

G

V
x
C

V
|
V

G

G

T

V

A

S

I

L

A

A

Y

Y

E

H

L

L

G

A

K

K

Y

A

N

G

L

M

-

K

D

D

V

V

L

L

E
|
E

K
|
K

G

S

D

E

D

L

V

T

A

A

S
x
G

G
x
S

T
|
T

T
x
W

K
x
H

A
|
A

N
x
A

-
x
G

-
x
L

S

T

A

T

I

Y

I

F

H

H

A

P

G

G

Y

I

D

N

A

L

K

K

P

-

G

-

T

-

L

-

K

-

A

-

K

K

L

I

N
x
H

V

Y

R

D

G

S

N

I

F

K

L

L

F

Y

S

E

K

K

I

L

C

E

E

E

L

T

D

G

-

Q

-

V

V

V

P

G

F

F

K

H

R

Q

I

P

G

G

S

S

L

I

V

R

L

L

A

A

F

T

N

T

D

P

E

V

E

R

I

V

K

D

E

E

I

F

E

K

N

Y

L

Q

L

M

E

T

R

R

G

T

K

G

I

W

N

H

G

A

I

T

P

E

Q

Q

I

Y

E

-

I

L

I

I

G

E

K

P

E

E

D

K

I

I

L

Q

K

E

M

M

E

F

P

P

-

L

N

N

V

M

N

N

K

K

E

-

V

V

K

L

A

A

A

G

L

L

F

Y

A

N

K

P

T

G

A

D

G

G

I

H

I

I

C

D

P

P

Y

Y

E

S

L

L

A

T

Q

M

A

A

F

L

G

A

E

A

N

G

A

A

F

R

L

K

N

C

G

G

V

A

E

L

F

L

K

K

F

Y

N

P

S

A

P

P

V
|
V

I

T

G

S

I

L

E

K

K

A

L

R

K

S

D

D

G

G

-

T

F

W

I

D

V

V

K

E

T

T

T

P

H

Q

E

G

D

S

I

M

H

R

A

A

R

N

F

R

I

I

I

V

N

N

A

A

G

G

V

F

Y

W

A

A

D

R

E

E

I

V

A

G

R

K

M

M

A

I

N

G

A

L

E

-

E

E

Y

H

K

P

I

L

I

I

P

P

R

V

K

Q

G

H

E

Q

Y

Y

L

V

F

T

D

S

K

T

S

I

V
x
S

G

E

N

V

I

K

V

A

N

L

K

K

V

R

L

E

F

L

P

P

T

V

P

L

T

R

K

D

I

L

S

E

K

G

G

S

I

Y

L

Y

V

L

S

R

P

Q

T

E

V

R

D

D

G

G

N

L

F

L

F

F

I

-

G

G

P

P

N

Y

S

E

N

S

N

Q

Q

E

E

K

S

M

K

K

-

V

E

Q

D

D

T

S

S

W

V

V

T

T

-

N

-

G

-

V

-

P

-

G

-

F

-

G

-

K

-

E

-

L

-

F

-

E

-

S

-

D

L

L

E

D

G

R

I

I

E

M

E

E

I

H

I

I

K

K

G

A

A

A

Q

M

R

E

L

M

V

V

P

P

N

V

I

L

P

K

L

K

K

A

S

D

V

I

I

I

T

N

S

V

F

V

A

N

G

G

I

P

R

I

A

T

V

Y

A

S

E

P

T

D

D

I

D

L

F

P

V

M

I

V

N

G

A

P

S

H

Q

Q

K

G

V

V

K

R

-

N

-

Y

-

W

-

V

-

A

-

I

-

G

-
x
F

-
x
G

-
x
Y

G
|
G

F
x
I

I
|
I

N

H

A

A

G

G

I

V

CV 59:60 (≠ VV 11:12) binding FAD
EK 80:81 (= EK 31:32) binding FAD
87:95 (vs. 38:44, 22% identical) binding FAD
H91 (≠ K42) modified: Tele-8alpha-FAD histidine
H109 (≠ N64) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V172) binding FAD
S279 (≠ V232) to P: in dbSNP:rs532964
FGYGII 397:402 (≠ ---GFI 328:330) binding FAD

Sites not aligning to the query:

530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
30% identity, 58% coverage: 3:283/488 of query aligns to 44:322/857 of Q63342

query
sites
Q63342

D

E

V

T

V

V

I

I

G

G

V
x
C

V
|
V

G

G

T

V

A

S

I

L

A

A

Y

Y

E

H

L

L

G

A

K

K

Y

A

N

G

L

M

-

R

D

D

V

V

L

L

E
|
E

K
|
K

G

S

D

E

D

L

V

T

A

A

S
x
G

G
x
S

T
|
T

T
x
W

K
x
H

A
|
A

N
x
A

-
x
G

-
x
L

S

T

A

T

I

Y

I

F

H

H

A

P

G

G

Y

I

D

N

A

L

K

K

P

-

G

-

T

-

L

-

K

-

A

-

K

K

L

I

N

H

V

Y

R

D

G

S

N

I

F

K

L

L

F

Y

S

E

K

R

I

L

C

E

E

E

L

T

D

G

-

Q

-

V

V

V

P

G

F

F

K

H

R

Q

I

P

G

G

S

S

L

I

V

R

L

L

A

A

F

T

N

T

D

P

E

E

E

R

I

V

K

D

E

E

I

F

E

K

N

Y

L

Q

L

M

E

T

R

R

G

T

K

N

I

W

N

H

G

A

I

T

P

E

Q

Q

I

Y

E

-

I

I

G

E

K

P

E

E

D

K

I

I

L

H

K

E

M

L

E

F

P

P

N

L

V

L

N

N

K

M

E

D

-

K

V

I

K

L

A

A

A

G

L

L

F

Y

A

N

K

P

T

G

A

D

G

G

I

H

I

I

C

D

P

P

Y

Y

E

S

L

L

A

T

Q

M

A

A

F

L

G

A

E

T

N

G

A

A

F

R

L

K

N

Y

G

G

V

V

E

L

F

L

K

K

F

Y

N

P

S

A

P

P

V
|
V

I

T

G

S

I

L

E

K

K

P

L

R

K

P

D

D

G

G

-

T

F

W

I

D

V

V

K

E

T

T

T

P

H

Q

E

G

D

S

I

V

H

R

A

A

R

N

F

R

I

I

I

V

N

N

A

A

G

G

V

F

Y
x
W

A

A

D

R

E

E

I

V

A

G

R

K

M

M

A

I

N

G

A

L

E

-

E

D

Y

H

K

P

I

L

I

I

P

P

R

V

K

Q

G

H

E

Q

Y

Y

L

V

F

T

D

S

K

T

S

I

V

P

G

E

N

V

I

K

V

A

N

L

K

K

V

R

L

E

F

L

P

P

T

V

P

L

T

R

K

D

I

L

S

E

K

G

G

S

I

Y

L

Y

V

L

S

R

P

Q

T

E

V

R

D

D

G

G

N

L

F

L

F

F

I

-

G

G

P

P

N

Y

S

E

N

S

N

Q

Q

E

E

K

S

M

K

K

E

L

D

Q

T

A

S

S

V

W

T

V

L

A

E

H

G

G

I

V

CV 52:53 (≠ VV 11:12) binding FAD
EK 73:74 (= EK 31:32) binding FAD
80:88 (vs. 38:44, 22% identical) binding FAD
H84 (≠ K42) modified: Tele-8alpha-FAD histidine
V212 (= V172) binding FAD
W244 (≠ Y203) binding FAD

Sites not aligning to the query:

390:395 binding FAD
573:575 binding (6S)-5,6,7,8-tetrahydrofolate
669 binding (6S)-5,6,7,8-tetrahydrofolate
676:678 binding (6S)-5,6,7,8-tetrahydrofolate
737 binding (6S)-5,6,7,8-tetrahydrofolate

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
30% identity, 58% coverage: 3:283/488 of query aligns to 7:285/824 of 4pabB

query
sites
4pabB

D

E

V

T

V

V

I

I

I
|
I

G
|
G

G

G

G
|
G

V
x
C

V
|
V

G

G

T

V

A

S

I

L

A

A

Y

Y

E

H

L

L

G

A

K

K

Y

A

N

G

L

M

-

R

D

D

V

V

L

L

L
|
L

E
|
E

K
|
K

G

S

D

E

D

L

V

T

A

A

S
x
G

G
x
S

T
|
T

T

W

K
x
H

A
|
A

N
x
A

-
x
G

-
x
L

S

T

A
x
T

I

Y

I

F

H

H

A

P

G

G

Y

I

D

N

A

L

K

K

P

-

G

-

T

-

L

-

K

-

A

-

K

K

L

I

N

H

V

Y

R

D

G

S

N

I

F

K

L

L

F

Y

S

E

K

R

I

L

C

E

E

E

L

T

D

G

-

Q

-

V

V

V

P

G

F

F

K

H

R

Q

I

P

G

G

S

S

L

I

V

R

L

L

A

A

F

T

N

T

D

P

E

E

E

R

I

V

K

D

E
|
E

I

F

E

K

N

Y

L

Q

L

M

E

T

R

R

G

T

K

N

I

W

N

H

G

A

I

T

P

E

Q

Q

I

Y

E

-

I

I

G

E

K

P

E

E

D

K

I

I

L

H

K

E

M

L

E

F

P

P

N

L

V

L

N

N

K

M

E

D

-

K

V

I

K

L

A

A

A

G

L

L

F

Y

A

N

K

P

T

G

A

D

G

G

I

H

I

I

C

D

P

P

Y

Y

E

S

L

L

A

T

Q

M

A

A

F

L

G

A

E

T

N

G

A

A

F

R

L

K

N

Y

G

G

V

V

E

L

F

L

K

K

F

Y

N

P

S

A

P

P

V
|
V

I

T

G

S

I

L

E

K

K

P

L

R

K

P

D

D

G

G

-

T

F

W

I

D

V

V

K

E

T

T

T

P

H

Q

E

G

D

S

I

V

H

R

A

A

R

N

F

R

I

I

I

V

N

N

A

A

A
|
A

G
|
G

V

F

Y
x
W

A

A

D

R

E

E

I

V

A

G

R

K

M

M

A

I

N

G

A

L

E

-

E

D

Y

H

K

P

I

L

I

I

P

P

R

V

K

Q

G
x
H

E

Q

Y
|
Y

L

V

F

T

D

S

K

T

S

I

V

P

G

E

N

V

I

K

V

A

N

L

K

K

V

R

L

E

F

L

P

P

T

V

P

L

T

R

K

D

I

L

S

E

K

G

G

S

I

Y

L
x
Y

V

L

S

R

P

Q

T

E

V

R

D

D

G

G

N

L

F

L

F

F

I

-

G

G

P

P

N

Y

S

E

N

S

N

Q

Q

E

E

K

S

M

K

K

E

L

D

Q

T

A

S

S

V

W

T

V

L

A

E

H

G

G

I

V

active site: T53 (≠ A46), E102 (= E99), H226 (≠ G223), Y255 (≠ L252)
binding flavin-adenine dinucleotide: I11 (= I7), G12 (= G8), G14 (= G10), C15 (≠ V11), V16 (= V12), L35 (= L30), E36 (= E31), K37 (= K32), G43 (≠ S38), S44 (≠ G39), T45 (= T40), H47 (≠ K42), A48 (= A43), A49 (≠ N44), G50 (vs. gap), L51 (vs. gap), V175 (= V172), A204 (= A200), G205 (= G201), W207 (≠ Y203), H226 (≠ G223), Y228 (= Y225)

Sites not aligning to the query:

active site: 536
binding flavin-adenine dinucleotide: 326, 328, 353, 355, 356, 357, 358
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
24% identity, 77% coverage: 14:390/488 of query aligns to 17:368/369 of S5FMM4

query
sites
S5FMM4

T

T

A

S

I

I

A

A

Y

Y

E

H

L

L

G

A

K

K

Y

A

N

G

L

K

D

K

V

T

V

A

L

V

L

F

E

E

K

S

G

G

D

E

D

-

V

V

A

G

S

K

G

K

T

A

T

T

K

S

A

A

N

A

S

A

A

G

I

M

I

L

-
x
G

-

A

H

H

A
|
A

G

E

Y

C

D

D

A

-

K

K

P

P

G

G

T

T

L

F

-

F

-

E

-

F

-

A

-

R

-

A

-

S

-

Q

K

K

A

A

K

Y

L

K

N

R

V

L

R

T

G

G

N

E

F

L

L

-

F

-

S

-

K
|
K

I

D

C

I

E

S

E

G

L

I

D

D

V

I

P

R

F

R

K

H

R

D

I

G

G

G

S

I

L

L

V

K

L

L

A

A

F

F

N

S

D

E

E

-

I

-

K

S

E

D

I

R

E

E

N

H

L

L

L

M

E

Q

R

M

G

G

K

A

I

L

N

D

G

S

I

V

P

E

Q

W

I

L

E

E

I

-

G

-

K

A

E

D

D

E

I

V

L

Y

K

K

M

L

E

E

P

P

N

N

V

A

N

G

K

K

E

G

V

I

K

L

A

G

A

A

L

N

F

F

A

I

K

R

T

D

A

D

G

V

I

H

I

V

C

E

P

P

Y

A

E

A

L

V

A

C

Q

R

A

A

F

F

G

A

E

R

N

G

A

A

F

R

L

M

N

L

G

G

V

A

E

D

F

V

K

F

F

E

N

Y

S

T

P

P

V

V

I

L

G

S

I

I

E

E

K

S

L

E

K

A

D

G

G

A

F

V

I

R

V

V

K

T

T

S

T

A

H

S

E

G

D

T

I

A

H

E

A

A

R

E

F

H

I

A

I

V

N

I

A

A

A
x
S

G

G

V

V

Y

W

A

S

D

G

E

A

I

L

A

F

R

K

M

Q

A

I

N

G

A

L

E

D

E

K

Y

-

K

R

I

F

I

Y

P

P

R

V

K

K

G

G

E

E

Y

C

L

L

L

-

F

-

D

-

K

-

S

-

V

-

G

-

N

S

I

V

V

W

N

N

K

D

V

G

L

I

F

S

P

L

T

T

P

R

T

T

K

L

I

Y

S

H

K

D

G

H

I

C

L

Y

V

I

S

V

P

P

T

R

V

H

D

S

G

G

N

R

F

L

F

V

I

V

G

G

P

A

N

T

S

M

N

K

N

P

Q

G

E

D

S

W

K

N

E

E

D

Q

T

P

S

E

V

-

T

-

L

L

E

G

G

G

I

I

E

E

I

L

I

I

K

R

G

K

A

A

Q

K

R

S

L

M

V

L

P

P

N

G

I

I

P

E

L

S

K

M

S

K

V

I

I

D

T

Q

S

C

F

W

A

A

G

G

I

L

R

R

A

P

V

E

A

T

E

G

T

D

D

G

D

N

F

P

V

Y

I

I

N

G

A

R

S

H

Q

P

K

E

V

N

K

D

G

R

F

I

I

L

N

F

A

A

G

A

G

G

I

H

Q

F

S

R

P

N

G

G

L
x
I

S

L

A

L

A

A

P

P

A

A

I

T

A

G

E

E

M

M

V
x
M

I

A

E

D

I

M

L

-

K

-

D

-

E

-

G

-

L

-

Q

-

L

-

N

-

Y

-

K

-

Q

-

N

-

Y

-

I

I

C

L

G

G

R

N

P

P

K

V

K

K

F

T

R

E

F

W

R

I

E

E

L

A

T

F

N

K

E

A

Q

E

R

R

K

K

K

E

L

A

I

V

E

H

G51 (vs. gap) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
A54 (= A50) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
K81 (= K73) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (≠ A200) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ L340) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ V350) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
21% identity, 73% coverage: 18:371/488 of query aligns to 21:363/364 of 3if9A

query
sites
3if9A

Y

Y

E

Y

L

L

G

A

K

K

Y

E

N

N

L

K

D

N

V

T

V

A

L

L

L

F

E
|
E

K
x
S

G

G

D

T

D

-

V

M

A

G

S

G

G

R

T
|
T

K

S

A

A

N
x
A

S
x
A

A
x
G

I
x
M

I

L

H

S

A

A

G

H

Y

R

D

E

A

C

K

E

P

E

G

R

T

D

L

A

K

F

A

F

K

D

L

F

N

A

V

M

R

H

G

S

N

Q

F

R

L

L

F

Y

S

K

K

G

I

L

C

G

E

E

L

L

-

Y

-

A

-

L

-

S

-

G

-

V

D

D

V

I

P

R

F

Q

K

H

R

N

I

G

G

G

S

M

L

F

V

K

L

L

A

A

F

F

N

S

D

E

E

E

E

D

I

V

K

-

E

-

I

-

E

-

N

-

L

-

L

L

E

Q

R

L

G

R

K

Q

I

M

N

D

G

D

I

L

P

D

Q

S

I

V

E

S

I

W

I

Y

G

S

K

K

E

E

D

E

I

V

L

L

K

E

M

K

E

E

P

P

N

Y

V

A

N

S

K

G

E

D

V

I

K

F

A

G

A

A

L

S

F

F

A

I

K

Q

T

D

A

D

G

V

I

H

I

V

C

E

P

P

Y

Y

E

F

L

V

A

C

Q

K

A

A

F

Y

G

V

E

K

N

A

A

A

F

K

L

M

N

L

G

G

V

A

E

E

F

I

K

F

F

E

N

H

S

T

P
|
P

V
|
V

I

L

G

H

I

V

E

E

K

R

L

D

K

G

D

E

G

A

F

L

I

F

V

I

K

K

T

T

T

P

H

S

E

G

D

D

I

V

H

W

A

A

R

N

F

H

I

V

N

V

A

A

A
x
S

G
|
G

V

V

Y
x
W

A

S

D

G

E

M

I
x
F

A

F

R

K

M

Q

A

L

N

G

A

L

E

N

Y

-

K

A

I

F

I

L

P

P

R

V

K

K

G

G

E

E

Y

C

L

L

L

-

F

-

D

-

K

-

S

-

V

-

G

-

N

S

I

V

V

W

N

N

K

D

V

D

L

I

F

P

P

L

T

T

P

K

T

T

K

L

I

Y

S

H

K

D

G

A

I

C

L
x
Y

V

I

S

V

P

P

T

R

V

K

D

S

G

G

N

R

F

L

F

V

I

V

G

G

P

A

N

T

S

M

N

K

N

P

Q

G

E

D

S

W

K

S

E

E

D

-

T

-

S

T

V

P

T

D

L

L

E

G

G

G

I

L

E

E

E

S

I

V

I

M

K

K

G

K

A

A

Q

K

R

T

L

M

V

L

P

P

N

A

I

I

P

Q

L

N

K

M

S

K

V

V

I

D

T

R

S

F

F

W

A

A

G
|
G

I

L

R
|
R

A

P

V

G

A

T

E

K

T

D

D

G

D

K

F

P

V

Y

I

I

N

G

A

R

S

H

Q

P

K

E

V

D

K

S

G

R

F

I

I

L

N

F

A

A

G

A

G

G

I
x
H

Q
x
F

S
x
R

P
x
N

G
|
G

L
x
I

S

L

A

L

A

A

P

P

A

A

I

T

A

G

E

A

M

L

V

I

I

S

E

D

I

L

L

I

K

M

D

N

E

K

G

E

L

V

Q

N

L

Q

N

D

Y

W

K

L

Q

H

N

A

Y

F

I

R

C

I

G

D

R

R

active site: A47 (≠ S45), G48 (≠ A46), M49 (≠ I47)
binding flavin-adenine dinucleotide: E34 (= E31), S35 (≠ K32), T42 (= T40), T43 (= T41), A46 (≠ N44), A47 (≠ S45), G48 (≠ A46), M49 (≠ I47), P173 (= P171), V174 (= V172), S202 (≠ A200), G203 (= G201), W205 (≠ Y203), F209 (≠ I207), G300 (= G308), R302 (= R310), H327 (≠ I335), F328 (≠ Q336), R329 (≠ S337), N330 (≠ P338), G331 (= G339), I332 (≠ L340)
binding glycolic acid: Y246 (≠ L252), R302 (= R310), R329 (≠ S337)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
21% identity, 73% coverage: 18:371/488 of query aligns to 21:363/364 of 1ng3A

query
sites
1ng3A

Y

Y

E

Y

L

L

G

A

K

K

Y

E

N

N

L

K

D

N

V

T

V

A

L

L

L
x
F

E
|
E

K
x
S

G

G

D

T

D

-

V

M

A

G

S

G

G
x
R

T
|
T

K

S

A

A

N
x
A

S
x
A

A
x
G

I
x
M

I

L

H

G

A

A

G

H

Y

A

D

E

A

C

K

E

P

E

G

R

T

D

L

A

K

F

A

F

K

D

L

F

N

A

V

M

R

H

G

S

N

Q

F

R

L

L

F

Y

S

K

K

G

I

L

C

G

E

E

L

L

-

Y

-

A

-

L

-

S

-

G

-

V

D

D

V

I

P
x
R

F

Q

K

H

R

N

I

G

G

G

S

M

L

F

V

K

L

L

A

A

F

F

N

S

D

E

E

E

E

D

I

V

K

-

E

-

I

-

E

-

N

-

L

-

L

L

E

Q

R

L

G

R

K

Q

I

M

N

D

G

D

I

L

P

D

Q

S

I

V

E

S

I

W

I

Y

G

S

K

K

E

E

D

E

I

V

L

L

K

E

M

K

E

E

P

P

N

Y

V

A

N

S

K

G

E

D

V

I

K

F

A

G

A

A

L

S

F

F

A

I

K

Q

T

D

A

D

G

V

I

H

I

V

C

E

P

P

Y

Y

E

F

L

V

A

C

Q

K

A

A

F

Y

G

V

E

K

N

A

A

A

F

K

L

M

N

L

G

G

V

A

E

E

F

I

K

F

F

E

N

H

S

T

P

P

V
|
V

I

L

G

H

I

V

E

E

K

R

L

D

K

G

D

E

G

A

F

L

I

F

V

I

K

K

T

T

T

P

H

S

E

G

D

D

I

V

H

W

A

A

R

N

F

H

I

V

N

V

A

A

A
x
S

G
|
G

V

V

Y
x
W

A

S

D

G

E

M

I
x
F

A

F

R

K

M

Q

A

L

N

G

A

L

E

N

Y

-

K

A

I

F

I

L

P

P

R

V

K

K

G

G

E

E

Y

C

L

L

L

-

F

-

D

-

K

-

S

-

V

-

G

-

N

S

I

V

V

W

N

N

K

D

V

D

L

I

F

P

P

L

T

T

P

K

T

T

K

L

I

Y

S

H

K

D

G

H

I

C

L
x
Y

V

I

S

V

P

P

T

R

V

K

D

S

G

G

N
x
R

F

L

F

V

I

V

G

G

P

A

N

T

S

M

N

K

N

P

Q

G

E

D

S

W

K

S

E

E

D

-

T

-

S

T

V

P

T

D

L

L

E

G

G

G

I

L

E

E

E

S

I

V

I

M

K

K

G

K

A

A

Q

K

R

T

L

M

V

L

P

P

N

P

I

I

P

Q

L

N

K

M

S

K

V

V

I

D

T

R

S

F

F

W

A

A

G
|
G

I

L

R
|
R

A

P

V

G

A

T

E

K

T

D

D

G

D

K

F

P

V

Y

I

I

N

G

A

R

S

H

Q

P

K

E

V

D

K

S

G

R

F

I

I

L

N

F

A

A

G

A

G

G

I
x
H

Q

F

S
x
R

P
x
N

G
|
G

L
x
I

S

L

A

L

A

A

P

P

A

A

I

T

A

G

E

A

M

L

V

I

I

S

E

D

I

L

L

I

K

M

D

N

E

K

G

E

L

V

Q

N

L

Q

N

D

Y

W

K

L

Q

H

N

A

Y

F

I

R

C

I

G

D

R

R

active site: A47 (≠ S45), G48 (≠ A46), M49 (≠ I47)
binding acetylamino-acetic acid: Y246 (≠ L252), R302 (= R310), R329 (≠ S337)
binding flavin-adenine dinucleotide: F33 (≠ L30), E34 (= E31), S35 (≠ K32), R41 (≠ G39), T42 (= T40), T43 (= T41), A46 (≠ N44), A47 (≠ S45), G48 (≠ A46), M49 (≠ I47), V174 (= V172), S202 (≠ A200), G203 (= G201), W205 (≠ Y203), F209 (≠ I207), G300 (= G308), R302 (= R310), H327 (≠ I335), R329 (≠ S337), N330 (≠ P338), G331 (= G339), I332 (≠ L340)
binding phosphate ion: R89 (≠ P81), R254 (≠ N260)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
21% identity, 73% coverage: 18:371/488 of query aligns to 21:363/369 of O31616

query
sites
O31616

Y

Y

E

Y

L

L

G

A

K

K

Y

E

N

N

L

K

D

N

V

T

V

A

L

L

L

F

E
|
E

K
x
S

G

G

D

T

D

-

V

M

A

G

S

G

G

R

T
|
T

K

S

A

A

N

A

S
x
A

A
x
G

I
x
M

I

L

H
x
G

A

A

G

H

Y
x
A

D

E

A

C

K

E

P

E

G

R

T

D

L

A

K

F

A

F

K

D

L

F

N

A

V

M

R

H

G

S

N

Q

F

R

L

L

F

Y

S

K

K

G

I

L

C

G

E

E

L

L

-

Y

-

A

-

L

-

S

-

G

-

V

D

D

V

I

P

R

F

Q

K

H

R

N

I

G

G

G

S

M

L

F

V

K

L

L

A

A

F

F

N

S

D

E

E

E

E

D

I

V

K

-

E

-

I

-

E

-

N

-

L

-

L

L

E

Q

R

L

G

R

K

Q

I

M

N

D

G

D

I

L

P

D

Q

S

I

V

E

S

I

W

I

Y

G

S

K

K

E

E

D

E

I

V

L

L

K

E

M

K

E

E

P

P

N

Y

V

A

N

S

K

G

E

D

V

I

K

F

A

G

A

A

L

S

F

F

A

I

K

Q

T

D

A

D

G

V

I

H

I

V

C

E

P

P

Y

Y

E

F

L

V

A

C

Q

K

A

A

F

Y

G

V

E

K

N

A

A

A

F

K

L

M

N

L

G

G

V

A

E

E

F

I

K

F

F

E

N

H

S

T

P

P

V
|
V

I

L

G

H

I

V

E

E

K

R

L

D

K

G

D

E

G

A

F

L

I

F

V

I

K

K

T

T

T

P

H

S

E

G

D

D

I

V

H

W

A

A

R

N

F

H

I

V

N

V

A

A

A

S

G

G

V

V

Y

W

A

S

D

G

E

M

I

F

A

F

R

K

M

Q

A

L

N

G

A

L

E

N

Y

-

K

A

I

F

I

L

P

P

R

V

K

K

G

G

E

E

Y

C

L

L

L

-

F

-

D

-

K

-

S

-

V

-

G

-

N

S

I

V

V

W

N

N

K

D

V

D

L

I

F

P

P

L

T

T

P

K

T

T

K

L

I

Y

S

H

K

D

G
x
H

I

C

L

Y

V

I

S

V

P

P

T

R

V

K

D

S

G

G

N

R

F

L

F

V

I

V

G

G

P

A

N

T

S

M

N

K

N

P

Q

G

E

D

S

W

K

S

E

E

D

-

T

-

S

T

V

P

T

D

L

L

E

G

G

G

I

L

E

E

E

S

I

V

I

M

K

K

G

K

A

A

Q

K

R

T

L

M

V

L

P

P

N

A

I

I

P

Q

L

N

K

M

S

K

V

V

I

D

T

R

S

F

F

W

A

A

G

G

I

L

R
|
R

A

P

V

G

A

T

E

K

T

D

D

G

D

K

F

P

V

Y

I

I

N

G

A

R

S

H

Q

P

K

E

V

D

K

S

G

R

F

I

I

L

N

F

A

A

G

A

G

G

I
x
H

Q
x
F

S
x
R

P
x
N

G
|
G

L
x
I

S
x
L

A

L

A

A

P

P

A

A

I

T

A

G

E

A

M

L

V

I

I

S

E

D

I

L

L

I

K

M

D

N

E

K

G

E

L

V

Q

N

L

Q

N

D

Y

W

K

L

Q

H

N

A

Y

F

I

R

C

I

G

D

R

R

ES 34:35 (≠ EK 31:32) binding FAD
TT 42:43 (= TT 40:41) binding FAD
AGM 47:49 (≠ SAI 45:47) binding FAD
G51 (≠ H49) mutation to R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; mutation to S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
A54 (≠ Y52) mutation to R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
V174 (= V172) binding FAD
H244 (≠ G250) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R310) binding substrate
327:333 (vs. 335:341, 14% identical) binding FAD
R329 (≠ S337) binding substrate

Sites not aligning to the query:

14:15 binding FAD

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
20% identity, 76% coverage: 3:371/488 of query aligns to 3:359/363 of 7cyxA

query
sites
7cyxA

D

D

V

V

V

A

I

I

I
|
I

G
|
G

G

G

G
|
G

V
|
V

V
x
I

G

G

T

S

A

S

I

V

A

A

Y

H

E

F

L

L

G

A

K

E

Y

R

N

G

L

H

D

K

V

V

V

A

L

I

L
x
V

E
|
E

K
|
K

G

-

D

Q

D

S

V

I

A

A

S

S

G
x
E

T
x
A

T
x
S

K

K

A

A

N
x
A

S

A

A
x
G

I
x
L

I

L

H

G

A

V

G

A

Y

Y

D

N

A

P

K

L

P

-

G

-

T

-

L

-

K

-

A

F

K

E

L

L

N

A

V

R

R

E

G

S

N

R

F

A

L

I

F

F

S

P

K

Q

I

L

C

A

E

A

E

V

L

L

-

R

-

E

-

K

-

T

-

G

-

V

D

D

V

I

P

G

F

Y

K

E

R

E

I

K

G

G

S

I

L

Y

V

R

L

I

A

A

F

Q

N

N

D

E

E

D

E

E

I

K

K

E

E

R

I

I

E

L

N

H

L

I

L

M

E

D

R

W

G

Q

K

Q

I

K

N

T

G

G

I

E

P

D

Q

S

I

Y

E

F

I

L

I

T

G

G

K

-

E

D

D

H

I

V

L

R

K

E

M

K

E

E

P

P

N

Y

V

L

N

S

K

E

E

S

V

I

K

I

A

G

A

A

L

V

F

Y

A

Y

K

P

T

K

A

D

G

G

I

H

I

V

C

I

P

A

Y

P

E

E

L

L

A

T

Q

K

A

A

F

F

G

A

E

H

N

S

A

A

F

A

L

I

N

S

G

G

V

A

E

D

F

I

K

Y

F

E

N

Q

S

T

P

E

V
|
V

I

F

G

D

I

I

E

-

K

R

L

I

K

E

D

N

G

N

F

K

I

V

-

T

-

G

V

V

K

I

T

T

T

S

H

E

E

G

D

I

I

V

H

T

A

C

R

E

F

K

I

V

N

I

A

A

A
x
G

G
|
G

V

S

Y
x
W

A

S

D

T

E

K

I

L

A

L

R

S

M

Y

A

F

N

H

A

-

E

R

E

D

Y

W

K

G

I

T

I

Y

P

P

R

V

K

K

G

G

E

E

Y

V

L

V

L

A

F

V

D

R

K

S

S

-

V

-

G

-

N

-

I

-

V

-

N

R

K

K

V

Q

L

L

F

L

P

K

T

A

P

P

T

I

K

-

I

F

S

Q

K

E

G

R

I

F

L

Y

V

I

S

T

P

P

T

K

V

R

D

G

G

G

N

R

F

Y

F

V

I

I

G

G

-

A

-

T

-

M

-

K

P

P

N

H

S

T

N

F

N

N

Q

K

E

-

S

-

K

-

E

-

D

-

T

-

S

T

V

V

T

Q

L

P

E

E

G

S

I

I

E

T

E

S

I

I

I

L

K

E

G

R

A

A

Q

Y

R

T

L

I

V

L

P

P

N

A

I

L

P

K

L

E

K

A

S

E

V

W

I

E

T

S

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A
x
P

V

Q

A

S

E

N

T

H

D

E

D

A

F

P

V

Y

I

M

N

G

A

E

S

H

Q

E

K

E

V

I

K

K

G

G

F

L

I

Y

N

A

A

C

G

T

G

G

I

H

Q
x
Y

S
x
R

P
x
N

G
|
G

L
x
I

S

L

A

L

A

S

P

P

A

I

I

S

A

G

E

Q

M

Y

V

M

I

A

E

D

I

L

L

I

K

-

D

-

E

E

G

G

L

K

Q

Q

L

E

N

N

Y

H

K

L

Q

L

N

D

Y

S

I

L

C

L

G

S

R

R

binding flavin-adenine dinucleotide: I7 (= I7), G8 (= G8), G10 (= G10), V11 (= V11), I12 (≠ V12), V30 (≠ L30), E31 (= E31), K32 (= K32), E38 (≠ G39), A39 (≠ T40), S40 (≠ T41), A43 (≠ N44), G45 (≠ A46), L46 (≠ I47), V171 (= V172), G200 (≠ A200), G201 (= G201), W203 (≠ Y203), G298 (= G308), R300 (= R310), P301 (≠ A311), Y326 (≠ Q336), R327 (≠ S337), N328 (≠ P338), G329 (= G339), I330 (≠ L340)

2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
31% identity, 43% coverage: 3:214/488 of query aligns to 21:238/403 of 2gagB

query
sites
2gagB

D

D

V

A

V

I

I

I

I

V

G
|
G

G

G

G
|
G

V
x
G

V
x
H

G

G

T

L

A

A

I

T

A

A

Y

Y

E

F

L

L

G

A

K

K

Y

N

N

H

-

G

-

I

L

T

D

N

V

V

V

A

L

V

L

L

E
|
E

K
|
K

G

G

D

W

D

-

V

L

A

A

S

G

G
|
G

T
x
N

T
x
M

K
x
A

A
x
R

N
|
N

S
x
T

A
x
T

I
|
I

I

I

H
x
R

A

S

G

N

Y

Y

-

L

-

W

D

D

A

E

K

S

P

A

G

G

T

I

L

Y

K

E

A

K

K

S

L

L

N

K

V

-

R

-

G

-

N

-

F

-

L

L

F

W

S

E

K

Q

I

L

C

P

E

E

E

D

L

L

-

E

-

Y

D

D

V

F

P

L

F

F

K

S

R

Q

I

R

G

G

S

V

L

L

V

N

L

L

A

A

F

H

N

T

D

L

E

G

E

D

I

V

K

R

E

E

I

S

E

V

N

R

L

R

L

V

E

E

R

A

G

N

K

K

I

L

N

N

G

G

I

V

P

-

Q

D

I

A

E

E

I

W

I

L

G

D

K

P

E

S

D

Q

I

V

L

K

K

E

M

A

E

C

P

P

N

I

V

I

N

N

K

T

E

S

-

D

-

I

-

R

-

Y

-

P

V

V

K

M

A

G

A

A

L

T

F

W

A

Q

K

P

T

R

A

A

G

G

I

I

I

A

C
x
K

P
x
H

Y

D

E

H

L

V

A

A

Q

W

A

A

F

F

G

A

E

R

N

K

A

A

F

N

L

E

N

M

G

G

V

V

E

D

F

I

K

I

F

Q

N

N

S

C

P

E

V
|
V

I

T

G

G

I

F

E

-

K

-

L

I

K

K

D

D

G

G

-

E

-

K

-

V

F

T

I

G

V

V

K

K

T

T

H

R

E

G

D

T

I

I

H

H

A

A

R

G

F

K

I

V

I

A

N

L

A

A

A
x
G

G
x
A

V

G

Y
x
H

A

S

D

S

E

V

I
x
L

A

A

R

E

M

M

A

A

N

G

A

F

E

E

active site: A61 (≠ K42), T64 (≠ S45), T65 (≠ A46)
binding flavin-adenine dinucleotide: G26 (= G8), G28 (= G10), G29 (≠ V11), H30 (≠ V12), E51 (= E31), K52 (= K32), G58 (= G39), N59 (≠ T40), M60 (≠ T41), R62 (≠ A43), N63 (= N44), T64 (≠ S45), I66 (= I47), V195 (= V172), G224 (≠ A200), A225 (≠ G201), H227 (≠ Y203), L231 (≠ I207)
binding flavin mononucleotide: A61 (≠ K42), R62 (≠ A43), H171 (≠ P148)
binding 2-furoic acid: T64 (≠ S45), I66 (= I47), R68 (≠ H49)
binding sulfite ion: K170 (≠ C147)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 246, 352, 353, 354, 355, 356, 357
binding flavin mononucleotide: 250, 278, 321, 323
binding 2-furoic acid: 263, 270, 357, 401
binding sulfite ion: 276

7rdfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f co-crystallized in the presence of d-arginine (see paper)
20% identity, 75% coverage: 3:366/488 of query aligns to 5:361/375 of 7rdfA

query
sites
7rdfA

D

D

V

Y

V

L

I

V

I
|
I

G
|
G

G

A

G
|
G

V
x
I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L
|
L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G
x
H

T
x
S

T
|
T

K

G

A
x
R

N
x
S

S
x
A

A

A

I
x
H

I

Y

H

T

A

V

G

A

Y
|
Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S
x
E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P
x
E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

A
|
A

G
|
G

V

A

Y
x
W

A

C

D

D

E

A

I

I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G
x
R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T

M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L
x
F

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I
x
Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: I9 (= I7), G10 (= G8), G12 (= G10), I13 (≠ V11), A14 (≠ V12), L31 (= L30), E32 (= E31), R33 (≠ K32), H40 (≠ G39), S41 (≠ T40), T42 (= T41), R44 (≠ A43), S45 (≠ N44), A46 (≠ S45), H48 (≠ I47), Y53 (= Y52), E87 (≠ S87), E170 (≠ P171), A171 (≠ V172), A199 (= A200), G200 (= G201), W202 (≠ Y203), R222 (≠ G223), F249 (≠ L252), G303 (= G308), R305 (= R310), Q330 (≠ I335), G331 (≠ Q336), G332 (≠ S337), Y333 (≠ P338), G334 (= G339), I335 (≠ L340), Q336 (≠ S341)
binding 6-hydroxy-flavin-adenine dinucleotide: I9 (= I7), G10 (= G8), G12 (= G10), I13 (≠ V11), A14 (≠ V12), E32 (= E31), R33 (≠ K32), H40 (≠ G39), S41 (≠ T40), T42 (= T41), S45 (≠ N44), A46 (≠ S45), H48 (≠ I47), E170 (≠ P171), A171 (≠ V172), A199 (= A200), G200 (= G201), W202 (≠ Y203), R222 (≠ G223), G303 (= G308), R305 (= R310), Q330 (≠ I335), G331 (≠ Q336), G332 (≠ S337), Y333 (≠ P338), G334 (= G339), I335 (≠ L340), Q336 (≠ S341)

6pldA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with 6-oh-fad - green fraction (see paper)
20% identity, 75% coverage: 3:366/488 of query aligns to 5:361/375 of 6pldA

query
sites
6pldA

D

D

V

Y

V

L

I

V

I
|
I

G
|
G

G

A

G
|
G

V
x
I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L
|
L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G
x
H

T
x
S

T
|
T

K

G

A
x
R

N
x
S

S
x
A

A

A

I
x
H

I

Y

H

T

A

V

G

A

Y

Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S

E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P
x
E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

A
|
A

G
|
G

V

A

Y
x
W

A

C

D

D

E

A

I

I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G

R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T

M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L

F

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I
x
Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

binding 6-hydroxy-flavin-adenine dinucleotide: I9 (= I7), G10 (= G8), G12 (= G10), I13 (≠ V11), A14 (≠ V12), L31 (= L30), E32 (= E31), R33 (≠ K32), H40 (≠ G39), S41 (≠ T40), T42 (= T41), R44 (≠ A43), S45 (≠ N44), A46 (≠ S45), H48 (≠ I47), E170 (≠ P171), A171 (≠ V172), A199 (= A200), G200 (= G201), W202 (≠ Y203), G303 (= G308), R305 (= R310), Q330 (≠ I335), G331 (≠ Q336), G332 (≠ S337), Y333 (≠ P338), G334 (= G339), I335 (≠ L340), Q336 (≠ S341)

6p9dA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with fad - yellow fraction (see paper)
20% identity, 75% coverage: 3:366/488 of query aligns to 5:361/375 of 6p9dA

query
sites
6p9dA

D

D

V

Y

V

L

I

V

I
|
I

G
|
G

G

A

G
|
G

V
x
I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L

L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G
x
H

T
x
S

T
|
T

K

G

A
x
R

N
x
S

S
x
A

A
|
A

I
x
H

I

Y

H

T

A

V

G

A

Y

Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S

E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P
x
E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

A
|
A

G
|
G

V

A

Y
x
W

A

C

D

D

E

A

I

I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G

R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T

M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L

F

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I
x
Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

binding dihydroflavine-adenine dinucleotide: I9 (= I7), G10 (= G8), G12 (= G10), I13 (≠ V11), A14 (≠ V12), E32 (= E31), R33 (≠ K32), H40 (≠ G39), S41 (≠ T40), T42 (= T41), R44 (≠ A43), S45 (≠ N44), A46 (≠ S45), A47 (= A46), H48 (≠ I47), E170 (≠ P171), A171 (≠ V172), A199 (= A200), G200 (= G201), W202 (≠ Y203), G303 (= G308), R305 (= R310), Q330 (≠ I335), G331 (≠ Q336), G332 (≠ S337), Y333 (≠ P338), G334 (= G339), I335 (≠ L340), Q336 (≠ S341)

Q9HXE3 FAD-dependent catabolic D-arginine dehydrogenase DauA; D-arginine dehydrogenase; DADH; D-arginine utilization protein A; Dau; EC 1.4.99.6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
20% identity, 75% coverage: 3:366/488 of query aligns to 5:361/375 of Q9HXE3

query
sites
Q9HXE3

D

D

V

Y

V

L

I

V

I

I

G

G

G

A

G

G

V

I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L

L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G

H

T
x
S

T
|
T

K
x
G

A
x
R

N
x
S

S
x
A

A
|
A

I
x
H

I

Y

H

T

A

V

G

A

Y

Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S
x
E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P

E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

G

G

V

A

Y

W

A

C

D

D

E

A

I

I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G

R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T

M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L

Y

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G

G

I

L

R

R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I

Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

A14 (≠ V12) binding FAD
ER 32:33 (≠ EK 31:32) binding FAD
41:48 (vs. 40:47, 25% identical) binding FAD
E87 (≠ S87) Important for specificity toward positively charged substrates
A171 (≠ V172) binding FAD
GGYGIQ 331:336 (≠ QSPGLS 336:341) binding FAD

3sm8A Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with an (n5) flavin adduct (see paper)
20% identity, 75% coverage: 3:366/488 of query aligns to 11:367/381 of 3sm8A

query
sites
3sm8A

D

D

V

Y

V

L

I

V

I
|
I

G
|
G

G

A

G
|
G

V
x
I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L
|
L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G
x
H

T
x
S

T
|
T

K

G

A
x
R

N
x
S

S
x
A

A

A

I
x
H

I

Y

H

T

A

V

G

A

Y
|
Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S

E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P
x
E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

A
|
A

G
|
G

V

A

Y
x
W

A

C

D

D

E

A

I
|
I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G
x
R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T
x
M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L
x
Y

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I
x
Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: I15 (= I7), G16 (= G8), G18 (= G10), I19 (≠ V11), A20 (≠ V12), L37 (= L30), E38 (= E31), R39 (≠ K32), H46 (≠ G39), S47 (≠ T40), T48 (= T41), R50 (≠ A43), S51 (≠ N44), A52 (≠ S45), H54 (≠ I47), Y59 (= Y52), E176 (≠ P171), A177 (≠ V172), A205 (= A200), G206 (= G201), W208 (≠ Y203), I212 (= I207), R228 (≠ G223), M246 (≠ T243), Y255 (≠ L252), G309 (= G308), R311 (= R310), Q336 (≠ I335), G337 (≠ Q336), G338 (≠ S337), Y339 (≠ P338), G340 (= G339), I341 (≠ L340), Q342 (≠ S341)

3nyfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with imino-histidine (see paper)
20% identity, 75% coverage: 3:366/488 of query aligns to 11:367/381 of 3nyfA

query
sites
3nyfA

D

D

V

Y

V

L

I

V

I
|
I

G
|
G

G

A

G
|
G

V
x
I

V
x
A

G

G

T

A

A

S

I

T

A

G

Y

Y

E

W

L

L

G

S

K

A

Y

H

N

G

L

-

D

R

V

V

L

V

L
|
L

E
|
E

K
x
R

G

E

D

A

D

Q

V

P

A

G

S

Y

G
x
H

T
x
S

T
|
T

K

G

A
x
R

N
x
S

S
x
A

A

A

I
x
H

I

Y

H
x
T

A

V

G

A

Y
|
Y

D

-

A

-

K

-

P

-

G

G

T

T

L

P

K

Q

A

V

K

R

L

A

N

L

V

T

R

A

G

A

N

S

F

R

L

A

F

F

-

F

-

D

-

N

-

P

S

A

K

G

I

F

C

C

E

E

E

H

-

P

L

L

D

L

V

S

P

P

F

R

K

P

R

-

I

-

G

-

S
x
E

L

M

V

V

L

V

A

D

F

F

N

S

D

D

E

D

E

P

I

-

K

E

E

E

I

L

E

R

N

R

L

Q

L

Y

E

E

R

S

G

G

K

K

I

A

N

L

G

-

I

V

P

P

Q

Q

I

M

E

R

I

L

G

D

K

A

E

E

D

Q

I

A

L

C

K

S

M

I

E

V

P

P

N

V

V

L

N

R

K

R

E

D

-

K

V

V

K

F

A

G

A

A

L

T

F

Y

A

D

K

P

T

T

A

G

G

A

I

D

I

I

C

D

P

T

Y

D

E

A

L

L

A

H

Q

Q

A

G

F

Y

G

L

E

R

N

G

A

I

F

R

L

R

N

N

G

Q

V

G

E

Q

F

V

K

L

F

C

N

N

S

H

P
x
E

V
x
A

I

L

G

E

I

I

E

R

K

R

L

V

K

D

D

G

G

A

F

W

I

E

V

V

K

R

T

C

T

D

H

A

E

G

D

S

I

Y

H

R

A

A

R

A

F

V

I

L

I

V

N

N

A

A

A
|
A

G
|
G

V

A

Y
x
W

A

C

D

D

E

A

I
|
I

A

A

R

G

M

L

A

A

N

G

A

V

E

R

E

P

Y

L

K

G

I

L

I

Q

P

P

R

K

K

R

G
x
R

E

S

Y

A

L

F

L

I

F

F

D

A

K

P

S

P

V

P

G

G

N

-

I

-

V

I

N

D

K

C

V

H

L

D

F

W

P

P

T
x
M

P

L

T

V

K

S

I

L

S

D

K

E

G

S

I

F

L
x
Y

V

L

S

K

P

P

T

-

V

-

D

D

G

A

N

G

F

M

F

L

I

L

G

G

P

S

N

P

S

A

N

N

N

A

-

D

-

P

-

V

-

E

-

A

-

H

-

D

-

V

Q

Q

E

P

S

E

K

Q

E

L

D

D

T

I

S

A

V

T

T

G

L

M

E

Y

G

L

I

I

E

E

I

A

I

T

K

-

G

-

A

-

Q

-

R

-

L

-

V

-

P

-

N

T

I

L

P

T

L

I

K

R

S

R

V

P

I

E

T

H

S

T

F

W

A

A

G
|
G

I

L

R
|
R

A

S

V

F

A

V

E

A

T

D

D

G

D

D

F

L

V

V

I

A

N

G

A

Y

S

A

Q

A

K

N

V

A

K

E

G

G

F

F

I

F

N

W

A

V

G

A

I
x
Q

Q
x
G

S
x
G

P
x
Y

G
|
G

L
x
I

S
x
Q

A

T

A

S

P

A

A

A

I

M

A

G

E

E

M

A

V

S

I

A

E

A

I

L

L

I

K

R

D

H

E

Q

G

P

L

L

Q

P

L

A

N

H

Y

L

K

R

Q

E

N

H

binding flavin-adenine dinucleotide: I15 (= I7), G16 (= G8), G18 (= G10), I19 (≠ V11), A20 (≠ V12), L37 (= L30), E38 (= E31), R39 (≠ K32), H46 (≠ G39), S47 (≠ T40), T48 (= T41), R50 (≠ A43), S51 (≠ N44), A52 (≠ S45), H54 (≠ I47), E176 (≠ P171), A177 (≠ V172), A205 (= A200), G206 (= G201), W208 (≠ Y203), I212 (= I207), G309 (= G308), R311 (= R310), Q336 (≠ I335), G337 (≠ Q336), G338 (≠ S337), Y339 (≠ P338), G340 (= G339), I341 (≠ L340), Q342 (≠ S341)
binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: H54 (≠ I47), T56 (≠ H49), Y59 (= Y52), E93 (≠ S87), R228 (≠ G223), M246 (≠ T243), Y255 (≠ L252), R311 (= R310), G338 (≠ S337)

Query Sequence

>WP_068748634.1 NCBI__GCF_001601575.1:WP_068748634.1
MKDVVIIGGGVVGTAIAYELGKYNLDVVLLEKGDDVASGTTKANSAIIHAGYDAKPGTLK
AKLNVRGNFLFSKICEELDVPFKRIGSLVLAFNDEEIKEIENLLERGKINGIPQIEIIGK
EDILKMEPNVNKEVKAALFAKTAGIICPYELAQAFGENAFLNGVEFKFNSPVIGIEKLKD
GFIVKTTHEDIHARFIINAAGVYADEIARMANAEEYKIIPRKGEYLLFDKSVGNIVNKVL
FPTPTKISKGILVSPTVDGNFFIGPNSNNQESKEDTSVTLEGIEEIIKGAQRLVPNIPLK
SVITSFAGIRAVAETDDFVINASQKVKGFINAGGIQSPGLSAAPAIAEMVIEILKDEGLQ
LNYKQNYICGRPKKFRFRELTNEQRKKLIEENPDFGHIVCRCETVTKAEIIDAIRRPLGA
KSLDAVKRRTRAGMGRCQGGFCSPRIAEILSKELNIEQTQVTKFGPGSEILKGRVKEFEI
EGGGHLAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory