SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_068749270.1 NCBI__GCF_001601575.1:WP_068749270.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

Q21A54 Phosphate propanoyltransferase; Phosphate acyltransferase PduL; Phosphotransacylase PduL; PTAC; Propanediol utilization protein PduL; EC 2.3.1.222 from Rhodopseudomonas palustris (strain BisB18) (see paper)
53% identity, 87% coverage: 23:206/211 of query aligns to 40:223/226 of Q21A54

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Q21A54

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VSN 44:46 (= VSN 27:29) binding CoA
H48 (= H31) binding Zn(2+)
H50 (= H33) binding Zn(2+)
M72 (≠ L54) binding CoA
K90 (= K72) binding CoA
R97 (= R79) binding CoA
R103 (= R85) binding phosphate
E109 (= E91) binding Zn(2+)
F116 (≠ Y98) binding CoA
H157 (= H139) binding Zn(2+)
H159 (= H141) binding Zn(2+)
H204 (= H187) binding Zn(2+)
N211 (= N194) binding CoA

Sites not aligning to the query:

1:33 mutation Missing: Enzyme active, produces crystals.

5cupA Structure of rhodopseudomonas palustris pdul - phosphate bound form (see paper)
53% identity, 87% coverage: 23:206/211 of query aligns to 5:188/189 of 5cupA

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binding phosphate ion: S17 (= S35), T19 (≠ E37), D20 (≠ A38), R68 (= R85), S92 (= S109), H124 (= H141)
binding zinc ion: H13 (= H31), H15 (= H33), E74 (= E91), H122 (= H139), H124 (= H141), H169 (= H187)

5cuoB Structure of rhodopseudomonas palustris pdul - coa bound form (see paper)
53% identity, 87% coverage: 23:206/211 of query aligns to 5:188/189 of 5cuoB

query
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5cuoB

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binding coenzyme a: V9 (= V27), S10 (= S28), N11 (= N29), H13 (= H31), H15 (= H33), M37 (≠ L54), K55 (= K72), R62 (= R79), L64 (≠ V81), A78 (≠ S95), F81 (≠ Y98), V172 (= V190), N176 (= N194)
binding zinc ion: H13 (= H31), H15 (= H33), E74 (= E91), H122 (= H139), H124 (= H141), H169 (= H187)

Q9XDN5 Phosphate propanoyltransferase; Phosphate acyltransferase PduL; Phosphotransacylase PduL; PTAC; Propanediol utilization protein PduL; EC 2.3.1.222 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
45% identity, 95% coverage: 1:200/211 of query aligns to 1:199/210 of Q9XDN5

query
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Q9XDN5

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DKEL 2:5 (≠ SQEI 2:5) mutation Missing: Decreased amount of PduL in BMCs, enzyme has wild-type activity.
2:10 (vs. 2:10, 33% identical) mutation Missing: Considerably decreased amount of PduL in BMCs, enzyme has greater than wild-type activity.

Query Sequence

>WP_068749270.1 NCBI__GCF_001601575.1:WP_068749270.1
MSQEILVSKITQEIISYINRVPVPVTVSNRHIHLSKEAKEILFGGKPLTVKKYLGQPGQF
AAEEVVKIIGPKGQFDAVRVVGPERKKCQVELSISDCYHLGINPIVRDSGKHEGTPGVLV
IGPKGFIKMEEGVIVAQRHIHMTPEDAEKYGVKDLQLVRIRFDSGPRRGILGDVLIRVDE
QFALECHLDVEEANALGIKNNDVVYMEIGGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory