SitesBLAST
Comparing WP_068865780.1 NCBI__GCF_001677335.1:WP_068865780.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
38% identity, 97% coverage: 4:409/418 of query aligns to 7:421/425 of O59010
- S65 (= S56) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ A269) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ ASS 269:271) binding L-aspartate
- M311 (= M305) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T308) binding L-aspartate
- V355 (≠ I349) binding L-aspartate
- D394 (= D382) binding L-aspartate
- M395 (= M383) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R385) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N389) binding L-aspartate
- D405 (= D393) mutation to N: Strongly decreased affinity for aspartate.
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
40% identity, 97% coverage: 9:414/418 of query aligns to 12:423/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K188), G195 (≠ V192), R282 (= R280)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A269), S272 (= S270), S273 (= S271), M307 (= M305), T310 (= T308), G353 (= G351), A354 (≠ S352), R394 (= R385), T395 (= T386)
Sites not aligning to the query:
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
40% identity, 97% coverage: 9:414/418 of query aligns to 13:424/425 of 6zgbA
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
40% identity, 97% coverage: 9:414/418 of query aligns to 15:426/426 of 6xwnB
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
40% identity, 97% coverage: 9:414/418 of query aligns to 15:426/427 of 5e9sA
- binding aspartic acid: R274 (≠ A269), S275 (= S270), S276 (= S271), T313 (= T308), G353 (= G348), V354 (≠ I349), A357 (≠ S352), G358 (= G353), D394 (= D382), R397 (= R385), T398 (= T386)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K188), G198 (≠ V192), Y202 (≠ L196)
- binding sodium ion: Y87 (≠ F81), T90 (= T84), S91 (≠ G85), S276 (= S271), G305 (= G300), A306 (= A301), T307 (= T302), N309 (= N304), N309 (= N304), M310 (= M305), D311 (≠ N306), S348 (≠ A343), I349 (= I344), G350 (= G345), T351 (≠ A346), N401 (= N389), V402 (≠ I390), D405 (= D393)
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
39% identity, 97% coverage: 9:414/418 of query aligns to 8:415/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ A269), S265 (= S271), M299 (= M305), T302 (= T308), T340 (≠ A346), G342 (= G348), V343 (≠ I349), G347 (= G353), D383 (= D382), R386 (= R385), T387 (= T386), N390 (= N389)
- binding decyl-beta-d-maltopyranoside: H23 (≠ Y25), V212 (≠ L217), A216 (≠ G221)
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
38% identity, 97% coverage: 4:407/418 of query aligns to 7:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y7 (≠ W4), F46 (≠ I37), F46 (≠ I37), P75 (≠ D66), L91 (≠ V83), F95 (≠ M87), L130 (≠ F130), I133 (≠ M133), I159 (≠ L159), Y167 (vs. gap), K196 (= K188), G200 (≠ V192), I207 (≠ F199), F210 (= F202), L250 (≠ M242), I262 (≠ F255), M269 (= M262), T334 (≠ N328), V335 (≠ W329), G336 (= G330), T340 (= T334), L343 (≠ I337), M399 (≠ T387)
- binding aspartic acid: S277 (= S270), S278 (= S271), T314 (= T308), G354 (= G348), A358 (≠ S352), G359 (= G353), D394 (= D382), R397 (= R385), T398 (= T386)
- binding sodium ion: Y89 (≠ F81), T92 (= T84), S93 (≠ G85), G306 (= G300), T308 (= T302), N310 (= N304), N310 (= N304), M311 (= M305), D312 (≠ N306), S349 (≠ A343), I350 (= I344), T352 (≠ A346), N401 (= N389), V402 (≠ I390), D405 (= D393)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
38% identity, 93% coverage: 4:393/418 of query aligns to 4:402/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ W4), G66 (= G60), V83 (≠ M78), I157 (≠ L160), Y164 (vs. gap), K193 (= K188), T305 (= T302), I306 (= I303), I347 (= I344)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V13), M199 (= M194), S275 (= S271), T311 (= T308), G356 (= G353), L384 (≠ A375), D391 (= D382), R394 (= R385)
2nwwA Crystal structure of gltph in complex with tboa (see paper)
38% identity, 92% coverage: 8:393/418 of query aligns to 2:396/407 of 2nwwA
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
38% identity, 95% coverage: 8:405/418 of query aligns to 3:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
38% identity, 92% coverage: 8:393/418 of query aligns to 3:397/408 of 6bauA