SitesBLAST

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
26% identity, 83% coverage: 107:712/734 of query aligns to 59:569/708 of Q46444

query
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E101 (= E149) binding pyrroloquinoline quinone
C147 (≠ S204) modified: Disulfide link with 148
C148 (= C205) modified: Disulfide link with 147
R153 (= R206) binding pyrroloquinoline quinone
T198 (= T272) binding pyrroloquinoline quinone
GA 214:215 (≠ QA 292:293) binding pyrroloquinoline quinone
E216 (= E294) binding Ca(2+)
T274 (≠ N339) binding pyrroloquinoline quinone
N294 (= N359) binding Ca(2+)
D339 (= D403) binding Ca(2+)
K366 (= K426) binding pyrroloquinoline quinone
NW 425:426 (≠ DW 526:527) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:31 signal peptide
575 binding pyrroloquinoline quinone
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
27% identity, 83% coverage: 103:713/734 of query aligns to 35:542/690 of Q8GR64

query
sites
Q8GR64

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Q

D
x
E

V
|
V

P
|
P

N
x
G

Y
x
V

N
x
Y

R
|
R

L
x
N

V
x
E

P
x
G

R
x
K

D
|
D

E
x
F

V
|
V

E
x
T

S
x
R

V

-

P

-

I

-

H

-

E

-

Q

-

E

-

S

-

G

-

A

-

Q
x
K

A
|
A

G
x
F

E
x
N

N
x
T

A
|
A

E
x
A

G
|
G

A
x
F

A
|
A

D
|
D

P
x
A

Q

-

A

-

G

-

S

-

P

-

Y

-

G

-

I

-

S

-

V

-

N

-

A

-

G

-

W

-

R

-

N

-

E

-

M

-

T
|
T

G
x
D

I
x
V

P
|
P

C
x
A

V
x
A

R
x
V

P
x
V

P
x
S

Y

-

G
|
G

H
x
A

I
x
L

R
x
L

A
|
A

I
x
W

D
|
D

L
x
P

E
x
V

T
x
K

G
x
Q

E
x
K

T
x
A

V
x
A

W
|
W

D
x
K

R
x
V

P
|
P

F
x
Y

G

-

T

-

A

-

R

-

N

-

G

-

P

-

F

-

G

-

I

-

P
|
P

S
x
T

Y
x
H

L
x
W

P

-

F

-

D

-

I

-

G

-

T

-

P

-

N

-

N
|
N

G
|
G

S
x
T

V
x
L

V
x
S

T
|
T

A
|
A

G
|
G

G
x
N

V
x
L

I
x
V

F
|
F

M
x
Q

G
|
G

A
x
T

A
|
A

T
x
A

D
x
G

N
x
Q

L
x
M

F

-

R
x
H

A
|
A

I
x
Y

D
x
S

V
x
A

E
x
D

T
x
K

G
|
G

E
|
E

T
x
A

L
|
L

W
|
W

Q
|
Q

D
x
F

V
x
E

L
x
A

P
x
Q

A
x
S

G
|
G

A
x
I

Q
x
V

A
|
A

N
x
A

P
|
P

I
x
M

T
|
T

Y
x
F

E
|
E

V
x
L

D
x
A

G
|
G

R
|
R

Q
|
Q

Y
|
Y

V
|
V

M
x
A

I
|
I

A
x
M

A
|
A

E81 (= E157) binding pyrroloquinoline quinone
C127 (≠ P200) modified: Disulfide link with 128
C128 (≠ Y201) modified: Disulfide link with 127
R133 (= R206) binding pyrroloquinoline quinone
T177 (= T272) binding pyrroloquinoline quinone
GA 193:194 (≠ QA 292:293) binding pyrroloquinoline quinone
E195 (= E294) binding Ca(2+)
T252 (≠ N339) binding pyrroloquinoline quinone
N272 (= N359) binding Ca(2+)
D317 (= D403) binding Ca(2+)
K344 (= K426) binding pyrroloquinoline quinone
NW 404:405 (≠ DW 526:527) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:22 signal peptide
23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
547 binding pyrroloquinoline quinone
613 binding covalent
616 binding covalent
617 binding axial binding residue
655 binding axial binding residue

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
27% identity, 83% coverage: 103:713/734 of query aligns to 13:520/664 of 1kv9A

query
sites
1kv9A

E

Q

V

A

G

G

A

G

D

E

W

W

P

L

F

S

W

H

G

G

G

R

D

T

A

Y

Q

A

A

E

T

Q

R

R

Y

F

S

S

P

P

L

L

D

K

E

Q

I

I

T

D

P

A

E

S

N

N

V

V

A

R

G

S

L

L

E

G

R

L

A

A

F

W

V

Y

Y

-

R

-

T

-

G

-

D

-

M

-

P

-

S

-

A

M

E

D

A

L

D

D

G

N

K

T

Y

R

S

G

P

L

E
|
E

T

A

T

T

P

P

L

L

K

F

I

H

G

D

D

G

D

V

L

I

L

Y

M

T

C

S

S

M

A

S

M

W

N

S

I

R

L

V

I

I

S

A

I

V

D

D

A

A

T

S

G

G

E

K

E

E

N

L

W

W

R

R

Y

Y

D

D

P

P

-

E

-

V

-

A

G

K

V

V

P

K

A
|
A

E
x
R

A

T

I

S

P
x
C

Y
x
C

G

D

A

A

S

V

C

N

R
|
R

G

G

V

V

S

A

I

L

Y

W

T

G

D

D

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R

K

V

V

I

Y

E

V

G

G

T

T

L

L

D

D

A

G

K

R

L

L

V

I

A

A

V

L

D

D

A

A

F

K

T

T

G

G

D

K

P

A

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

-

D

-

L

I

W

W

Q

S

D

Q

I

Q

G

T

E

T

R

D

V

P

P

A

G

K

W

P

Y

Y

S

S

V

I

T
|
T

A

G

P

A

P

P

A

R

V

V

R

K

G

G

I

K

L

V

V

I

T

I

G

G

A

-

Q

-

V

-

K

-

D

N

G
|
G

Q

G

A
|
A

E
|
E

D

Y

A

G

P

V

S

R

G

G

V

F

V

V

R

S

G

A

Y

Y

D

D

A

A

V

D

T

T

G

G

D

K

L

L

V

A

W

W

-

R

-

F

-

Y

-

T

-

V

-

P

-

G

-

D

-

P

A

A

W

L

D

P

L

Y

A

E

A

H

P

P

E

E

A

L

N

R

R

E

A

A

G

A

P

K

P

T

-

W

A

Q

G

G

E

D

V

Q

Y

Y

T

W

R

K

-

L

-

G

G

G

T

G

P

G

N
x
T

M

V

W
|
W

T

D

T

S

A

M

V

A

A

Y

D

D

E

P

E

E

L

L

G

D

Y

L

V

L

Y

Y

L

V

P

G

L

T

G

G

N
|
N

S

G

S

S

V

-

D

P

Y

W

Y

N

G

R

S

E

N

V

R

R

S

S

-

P

-

G

E

G

A

G

E

D

N

N

A

L

F

Y

A

L

T

S

S

S

L

I

V

L

A

A

L

I

D

R

A

P

T

D

T

T

G

G

E

K

E

L

V

A

W

W

H

H

F

Y

Q

Q

T

V

V

T

R

P

H

G

D

D

V

S

W

W

D
|
D

Y

F

D

T

L

A

G

T

S

Q

Q

Q

P

I

T

T

L

L

-

A

-

E

L

L

D

N

W

I

Q

D

G

G

T

K

P

P

A

R

I

K

L

V

L

L

P

M

S

Q

-

A

-

P

K
|
K

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

L

D

D

R

R

A

T

T

N

G

G

E

K

P

-

L

L

T

I

P

S

M

A

E

E

E

K

L

F

T

-

G

-

L

-

P

-

Q

-

G

G

D

K

V

V

E

-

P

-

D

-

Y

-

I

-

S

-

P

-

T

-

Q

-

P

-

A

-

S

-

A

T

W

W

A

A

-

E

T

K

L

V

R

D

M

L

P

A

P

T

L

G

E

R

P

P

R

V

D

E

A

A

W

P

G

G

F

-

T

-

A

-

I

-

D

-

Q

-

L

-

W

-

C

-

R

-

I

-

Q

-

F

-

H

-

R

-

A

V

N

R

Y

Y

E

E

G

-

Y

-

F

-

T

-

P

-

S

-

D

K

R

E

P

P

W

I

I

V

Q

M

Y

W

P

P

G

S

Y

P

N

F

G

G

G

A

S

H

D
x
N

W
|
W

G

H

S

S

V

M

A

S

V

F

D

N

S

P

Q

G

R

T

G

G

L

L

I

V

I

Y

A

I

N

P

Y

Y

N

Q

D

E

V

V

P

P

N

G

Y

V

N

Y

R

R

L

N

V

E

P

G

R

K

D

D

E

F

V

V

E

T

S

R

V

-

P

-

I

-

H

-

E

-

Q

-

E

-

S

-

G

-

A

-

Q

K

A

A

G

F

E

N

N

T

A

A

E

A

G

G

A

F

A

A

D

D

P

A

Q

-

A

-

G

-

S

-

P

-

Y

-

G

-

I

-

S

-

V

-

N

-

A

-

G

-

W

-

R

-

N

-

E

-

M

-

T

T

G

D

I

V

P

P

C

A

V

A

R

V

P

V

P

S

Y

-

G

G

H

A

I

L

R

L

A

A

I

W

D

D

L

P

E

V

T

K

G

Q

E

K

T

A

V

A

W

W

D

K

R

V

P

P

F

Y

G

-

T

-

A

-

R

-

N

-

G

-

P

-

F

-

G

-

I

-

P

P

S

T

Y

H

L
x
W

P

-

F

-

D

-

I

-

G

-

T

-

P

-

N

-

N

N

G

G

S

T

V

L

V

S

T

T

A

A

G

G

G

N

V

L

I

V

F

F

M

Q

G

G

A

T

A

A

T

A

D

G

N

Q

L

M

F

-

R

H

A

A

I

Y

D

S

V

A

E

D

T

K

G

G

E

E

T

A

L

L

W

W

Q

Q

D

F

V

E

L

A

P

Q

A

S

G

G

A

I

Q

V

A

A

N

A

P

P

I

M

T

T

Y

F

E

E

V

L

D

A

G

G

R

R

Q

Q

Y

Y

V

V

M

A

I

I

A

M

A

A

active site: E173 (= E294), N250 (= N359), D295 (= D403)
binding acetone: E173 (= E294), D295 (= D403)
binding calcium ion: E173 (= E294), N250 (= N359), D295 (= D403)
binding heme c: A101 (= A196), R102 (≠ E197)
binding pyrroloquinoline quinone: E59 (= E157), C105 (≠ P200), C106 (≠ Y201), R111 (= R206), T155 (= T272), G170 (= G291), A172 (= A293), E173 (= E294), T230 (≠ N339), W232 (= W341), K322 (= K426), N382 (≠ D526), W383 (= W527), W460 (≠ L644)

Sites not aligning to the query:

binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
binding pyrroloquinoline quinone: 525

C5AXV8 Lanthanide-dependent ethanol dehydrogenase; Lanthanide-dependent EtDH; Ln-dependent EtDH; Lanthanide-dependent formaldehyde dehydrogenase; PQQ-dependent ethanol dehydrogenase; EC 1.1.2.-; EC 1.2.2.- from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) (see paper)
25% identity, 81% coverage: 116:713/734 of query aligns to 51:549/587 of C5AXV8

query
sites
C5AXV8

Q

Q

A

A

T

Q

R

R

Y

F

S

S

P

P

L

I

D

K

E

T

I

L

T

N

P

R

E

D

N

N

V

I

A

K

G

G

L

L

E

V

R

P

A

A

F

W

V

S

Y

F

R

S

T

F

G

G

D

-

M

-

P

-

S

-

A

-

E

-

A

G

D

E

G

K

K

Q

Y

R

S

G

P

Q

E

E

T

S

T

Q

P

A

L

L

K

V

I

K

G

D

D

G

D

V

L

L

L

Y

M

V

C

T

S

G

A

S

M

Y

N

S

I

R

L

L

I

Y

S

A

I

V

D

D

A

A

A

R

T

T

G

G

E

E

E

K

N

K

W

W

R

E

Y

Y

D

N

P

A

G

R

V

L

P

P

A

E

E

G

A

I

I

L

P

P

Y

-

G

-

A

-

S

C

C

C

-

D

-

V

-

N

R

R

G

G

V

A

S

A

I

L

Y

Y

T

K

D

D

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R

K

V

V

I

Y

E

F

G

G

T

T

L

L

D

D

A

A

K

K

L

L

V

V

A

A

V

L

D

D

A

Q

F

K

T

T

G

G

D

K

P

-

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

V

D

V

L

W

W

R

Q

K

D

D

I

I

G

D

E

D

R

F

V

K

P

S

G

G

W

-

Y

Y

S

S

V

Y

T

T

A

A

P

A

P

P

A

M

V

I

V

V

R

K

G

G

I

K

L

I

V

I

T

T

G

G

A

V

Q

S

V

-

K

-

D

-

G

-

Q

G

A

G

E

E

D

F

A

G

P

V

S

V

G

G

V

R

V

V

R

E

G

A

Y

R

D

D

A

A

V

E

T

T

G

G

D

E

L

V

V

V

W

W

A

K

W

R

-

P

-

V

-

I

-

E

-

G

-

H

-

M

-

G

-

E

-

L

-

N

-

G

-

K

-

P

D

S

L

T

A

M

A

T

P

G

E

K

A

A

N

N

R

E

A

S

G

W

P

P

P

-

A

-

G

G

E

D

V

T

Y

W

T

K

R

F

G

G

T

G

P

G

N

A

M

T

W

W

T

L

T

G

A

G

V

T

A

Y

D

D

E

P

E

E

L

L

G

N

Y

L

V

I

Y

Y

L

F

P

G

L

T

G

G

N

N

S

P

S

G

V

P

D

-

Y

-

Y

W

G

N

S

S

N

A

R

L

S

R

E

P

A

G

E

D

N

N

A

K

F

W

A

S

T

A

S

S

L

R

V

L

A

A

L

I

D

N

A

P

T

D

T

N

G

G

E

E

E

I

V

V

W

W

H

G

F

F

Q

Q

T

T

V

T

R

P

H

H

D

D

V

G

W

W

D

D

Y

Y

D
|
D

L

G

G

V

S

N

Q

E

P

F

T

V

L

P

L

F

D

D

W

M

Q

Q

G

K

-

D

-

G

-

K

-

T

T

V

P

K

A

A

I

G

L

A

L

T

P

A

S

D

K

R

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

L

D

D

R

R

A

T

T

N

G

G

E

A

P

F

L

L

-

S

-

A

T

T

P

P

M

F

E

V

E

S

L

N

T

I

G

N

L

W

P

A

Q

K

G

G

D

I

V

D

E

E

-

N

-

G

-

R

P

P

D

I

Y

Y

I

D

S

D

P

A

T

N

Q

R

P

P

A

G

S

D

A

-

W

-

A

-

T

-

L

-

R

-

M

-

P

-

P

P

L

A

E

K

P

S

R

E

D

D

A

G

W

-

G

-

F

-

T

-

A

-

I

-

D

-

Q

-

L

-

W

-

C

-

R

-

I

-

Q

-

F

-

H

-

R

K

A

K

N

G

Y

K

E

S

G

V

Y

Y

F

A

T

T

P

-

S

-

D

-

R

-

P

-

W

-

I

-

Q

-

Y

-

P

P

G

G

Y

F

N

L

G

G

S

K

D

N

W

W

G

N

S

P

V

I

A

A

V

Y

D

S

S

P

Q

D

R

T

G

R

L

L

I

F

I

-

A

-

N

-

Y

-

N

-

D

-

V

-

P

-

N

-

Y

-

N

-

R

-

L

Y

V

V

P

P

R

S

D

N

E

E

-

W

-

G

-

M

-

D

V

I

E

W

S

N

V

E

P

P

I

V

H

N

E

Y

Q

K

E

K

S

G

G

A

A

A

Q

Y

A

L

G

G

E

A

N

-

A

-

E

-

G

-

A

-

D

-

P

-

Q

-

A

-

G

-

S

-

P

-

Y

-

G

G

I

F

S

T

V

I

N

K

A

-

G

-

W

-

R

-

N

-

E

-

M

-

T

-

G

-

I

-

P

P

C

T

V

F

R

E

P

D

P

H

Y

I

G

G

H

S

I

L

R

K

A

A

I

I

D

D

L

P

E

A

T

T

G

K

E

K

T

V

V

V

W

W

D

E

R

-

P

-

F

-

G

-

T

-

A

-

R

-

N

-

G

-

P

-

F

-

G

-

I

-

P

-

S

-

Y

-

L

-

P

-

F

Y

D

K

I

N

G

K

T

A

P

P

N

L

N

W

G

A

G

G

S

V

V

L

V

T

T

T

A

A

G

G

G

N

V

L

I

V

F

F

M

T

G

G

A

T

A

P

T

-

D

E

N

G

L

F

F

L

R

K

A

A

I

F

D

D

V

A

E

K

T

T

G

G

E

E

T

E

L

V

W

W

Q

K

D

F

V

Q

L

V

P

G

A

T

G

G

A

V

Q

V

A

A

N

S

P

P

I

I

T

T

Y

W

E

E

V

Q

D

D

G

G

R

E

Q

Q

Y

W

V

I

M

G

I

V

A

A

D319 (= D405) mutation to S: Loss of efficient ethanol oxidation with La(3+).

8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
26% identity, 82% coverage: 107:711/734 of query aligns to 19:547/723 of 8gy2A

query
sites
8gy2A

D

D

W

W

P

L

F

S

W

Y

G

G

G

R

D

S

A

Y

Q

S

A

E

T

Q

R

R

Y

Y

S

S

P

P

L

L

D

D

E

Q

I

I

T

N

P

T

E

E

N

N

V

V

A

G

G

K

L

L

E

K

R

L

A

A

F

W

V

H

Y

Y

R

-

T

-

G

-

D

-

M

-

P

-

S

-

A

-

E

D

A

L

D

D

G

T

K

N

Y

R

S

G

P

Q

E

E

T

G

T

T

P

P

L

L

K

I

I

V

G

N

D

G

D

V

L

M

L

Y

M

A

C

T

S

T

A

N

M

W

N

S

I

K

L

M

I

K

S

A

I

L

D

D

A

A

T

T

G

G

E

K

E

L

N

L

W

W

R

S

Y

Y

D

D

P

P

G

K

V

V

P

P

A

G

E

N

A

I

I

A

P
x
D

Y

R

G

G

A
x
C

S
x
C

C

-

R

-

G

-

V

-

S

-

I

-

Y

-

T

-

D

D

P

T

A

V

A

S

A

R

E

G

G

A

T

A

L

Y

C

W

A

N

T

G

R

K

V

V

I

Y

E

F

G

G

T

T

L

F

D

D

A

G

K

R

L

L

V

I

A

A

V

L

D

D

A

A

F

K

T

T

G

G

D

K

P

L

C

V

E

W

G

S

F

V

G

Y

E

-

N

-

G

-

T

T

V

I

D

P

L

K

W

E

Q

A

D

Q

I

L

G

G

E

H

R

Q

V

R

P

S

G

-

W

-

Y

Y

S

T

V

V

T
x
D

A

G

P

A

P

P

A

R

V

I

V

A

R

K

G

G

I

K

L

V

V

L

T

I

G

G

A

-

Q

-

V

-

K

-

D

N

G

G

Q
x
G

A
|
A

E
|
E

D

F

A

G

P

A

S

R

G

G

V

F

V

V

R

S

G

A

Y

F

D

D

A

A

V

E

T

T

G

G

D

K

L

L

V

D

W

W

A

R

W

F

-

F

D

T

L

V

A

P

A

N

P

P

E

E

A

N

N

K

R

P

A

D

G

G

P

A

A

S

G

D

E

D

V

I

Y

L

T

M

R

S

-

K

-

A

-

Y

-

P

-

T

-

W

-

G

-

K

-

N

-

G

-

A

-

W

-

K

-

Q

-

G

G

G

T

G

P

G

N

T

M

V

W
|
W

T

D

T

S

A

L

V

V

A

Y

D

D

E

P

E

V

L

T

G

D

Y

L

V

V

Y

Y

L

L

P

G

L

V

G

G

N
|
N

S

G

S

S

-

P

V

W

D

N

Y

Y

Y

K

G

F

S

R

N

S

R

E

S

G

E

K

A

G

E

D

N

N

A

L

F

F

A

L

T

G

S

S

L

I

V

V

A

A

L

I

D

N

A

P

T

D

T

T

G

G

E

K

E

Y

V

V

W

W

H

H

F

F

Q

Q

T

E

V

T

R

P

H

M

D

D

V

E

W

W

D
|
D

Y

Y

D

T

L

S

G

V

S

Q

Q

Q

P

I

T

M

L

T

L

L

D

D

W

M

-

P

-

V

-

N

-

G

Q

E

G

M

T

R

P

H

A

V

I

I

L

V

L

H

P

A

S

P

K
|
K

Q

N

G

G

D

F

L

F

Y

Y

I

I

L

I

D

D

R

A

A

K

T

T

G

G

E

-

P

-

L

-

T

-

P

-

M

-

E

K

E

F

L

I

T

T

G

G

L

K

P

P

Q

Y

G

-

D

-

V

T

E

Y

P

E

D

N

Y

W

I

A

S

N

P

G

T

L

Q

D

P

P

A

V

S

T

A

G

W

-

A

-

T

-

L

-

R

-

M

R

P

P

P

N

L

Y

E

V

P

P

R

D

D

A

A

L

W

W

G

T

F

L

T

T

A

G

I

-

D

-

Q

-

L

-

W

-

C

-

R

-

I

-

Q

-

F

-

H

-

R

-

A

-

N

-

Y

-

E

-

G

-

Y

-

F

-

T

-

P

-

S

-

D

-

R

K

P

P

W

W

I

L

Q

G

Y

I

P

P

G

G

Y

E

N

L

G

G

S

H

D

N

W
x
F

G

A

S

A

V

M

A

A

V

Y

D

S

S

P

Q

K

R

T

G

K

L

L

I

V

I

Y

A

I

N

P

Y

A

N

Q

D

Q

V

I

P

P

N

-

Y

-

N

-

R

-

L

-

V

-

P

-

R

-

D

-

E

-

V

-

E

-

S

-

V

L

P

L

I

Y

H

D

E

G

Q

Q

E

K

S

G

G

G

A

F

Q

K

A

A

G

-

E

-

N

-

A

-

E

-

G

-

A

-

D

-

P

-

Q

-

A

-

G

-

S

-

P

-

Y

Y

G

H

I

D

S

A

V

W

N

N

A

L

G

G

W

L

R

D

N

M

E

N

M

K

T

I

G

G

I

L

P

F

C

D

V

D

R

N

P

D

P

P

Y

-

G

E

H

H

I

V

R

A

A

A

-

K

-

D

-

F

I

L

D

K

L

V

E

L

T

K

G

G

E

W

T

T

V

V

-

A

W

W

D

D

-

P

-

E

-

K

-

M

R

A

P

P

F

A

G

F

T

T

A

I

R

N

R

H

N

K

G

G

P

P

F
x
W

G

-

I

-

P

-

S

-

Y

-

L

-

P

-

F

-

D

-

I

-

G

-

T

-

P

-

N

-

N

N

G

G

S

L

V

L

V

A

T

T

A

A

G

G

G

N

V

V

I

I

F

F

M

Q

G

G

A

L

A

A

T

-

D

N

N

G

L

E

F

F

R

H

A

A

I

Y

D

D

V

A

E

T

T

N

G

G

E

N

T

D

L

L

W

Y

Q

S

D

F

V

P

L

A

P

Q

A

S

G

A

A

I

Q

I

A

A

N

P

P

P

I

V

T

T

Y

Y

E

T

V

A

D

N

G

G

R

K

Q

Q

Y

Y

V

V

M

A

I

V

binding calcium ion: E181 (= E294), N263 (= N359), D308 (= D403)
binding heme c: D104 (≠ P200)
binding pyrroloquinoline quinone: C107 (≠ A203), C108 (≠ S204), D163 (≠ T272), G179 (≠ Q292), A180 (= A293), E181 (= E294), W245 (= W341), N263 (= N359), D308 (= D403), K335 (= K426), F398 (≠ W527), W489 (≠ F638)

Sites not aligning to the query:

binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
29% identity, 46% coverage: 107:442/734 of query aligns to 50:372/718 of Q4W6G0

query
sites
Q4W6G0

D
x
N

W
|
W

P
x
M

F
x
S

W
x
T

G
|
G

G
x
R

D
x
T

A
x
Y

Q
x
D

A
x
E

T
x
Q

R
|
R

Y
|
Y

S
|
S

P
|
P

L
|
L

D
x
K

E
x
Q

I
|
I

T
x
S

P
x
D

E
x
Q

N
|
N

V
|
V

A
x
G

G
x
Q

L
|
L

E
x
G

R
x
L

A
|
A

F
x
W

V
x
S

Y
|
Y

R
x
K

T
x
L

G

-

D

-

M

-

P

-

S

-

A

-

E

-

A

-

D
|
D

G
x
L

K
x
D

Y
x
R

S
x
G

P
x
V

E
|
E

T
x
A

T
|
T

P
|
P

L
x
I

K
x
V

I
x
V

G
x
D

D
x
G

D
x
A

L
x
M

L
x
Y

M
x
T

C
x
T

S
x
G

A
x
P

M
x
F

N
x
S

I
x
V

L
x
V

I
x
Y

S
x
A

I
x
L

D
|
D

A
|
A

A
x
R

T
x
D

G
|
G

E
x
R

E
x
L

N
x
I

W
|
W

R
x
K

Y
|
Y

D
|
D

P
|
P

G

-

V

-

P
x
Q

A
x
S

E
x
D

A
x
R

I
x
H

P
x
R

Y
x
A

G
|
G

A
x
E

S
x
A

C
|
C

-
x
C

-
x
D

-
x
A

-
x
V

-
x
N

R
|
R

G
|
G

V
|
V

S
x
A

I
x
V

Y
x
W

T
x
K

D
x
G

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R
x
K

V
|
V

I
x
Y

E
x
V

G
|
G

T
x
V

L
|
L

D
|
D

A
x
G

K
x
R

L
|
L

V
x
E

A
|
A

V
x
I

D
|
D

A
|
A

F
x
K

T
|
T

G
|
G

D
x
Q

P
x
R

C
x
A

E
x
W

G

-

F

-

G

-

E

-

N

-

G

-

T
x
S

V
|
V

D
|
D

L
x
T

W
x
R

Q
x
A

D
|
D

I
x
H

G
x
K

E
x
R

R
x
S

V

-

P

-

G

-

W

-

Y
|
Y

S
x
T

V
x
I

T
|
T

A
x
G

P
x
A

P
|
P

A
x
R

V
|
V

R
x
N

G
|
G

I
x
K

L
x
V

V
|
V

T
x
I

G
|
G

A

-

Q

-

V

-

K

-

D
x
N

G
|
G

Q
x
G

A
|
A

E
|
E

D
x
F

A
x
G

P
x
V

S
x
R

G
|
G

V
x
Y

V
|
V

R
x
T

G
x
A

Y
|
Y

D
|
D

A
|
A

V
x
E

T
|
T

G
|
G

D
x
K

L
x
E

V

-

W

-

A
|
A

W
|
W

D
x
R

L
x
F

A
x
Y

A
x
T

P
x
V

E
x
P

A
x
G

N
x
D

R
x
P

A
x
K

G
x
L

P
|
P

A
x
E

G
|
G

E
x
K

-
x
G

-
x
M

-
x
E

-
x
I

-
x
A

-
x
K

-
x
T

-
x
W

-
x
F

-
x
G

-
x
D

-
x
A

-
x
Y

V
|
V

Y
x
E

T
x
Q

R
x
G

G
|
G

T
x
G

P
x
G

N
x
T

M
x
A

W
|
W

T
x
D

T
x
S

A
x
F

V
x
A

A
x
Y

D
|
D

E
x
P

E
|
E

L
|
L

G
x
N

Y
x
L

V
x
L

Y
|
Y

L
x
I

P
x
G

L
x
V

G
|
G

N
|
N

S
x
G

S
|
S

V
x
L

-
x
W

D
|
D

Y
x
P

Y
x
K

G
x
W

S
x
R

N
x
S

R
x
Q

S
x
A

E
x
K

A
x
G

E
x
D

N
|
N

A
x
L

F
|
F

A
x
L

T
x
S

S
|
S

L
x
I

V
|
V

A
|
A

L
x
V

D
x
N

A
|
A

T
x
D

T
|
T

G
|
G

E
|
E

E
x
Y

V
|
V

W
|
W

H
|
H

F
x
Y

Q
|
Q

T
|
T

V
x
T

R
x
P

H
x
G

D
|
D

V
x
A

W
|
W

D
|
D

Y
|
Y

D
x
T

L
x
A

G
x
T

S
x
Q

Q
x
H

P
x
M

T
x
I

L
|
L

-
x
A

-
x
E

-
x
L

-
x
P

L
x
I

D
|
D

W
x
G

Q
x
K

G
x
P

T
x
R

P
x
K

A
x
V

I
x
L

L
x
M

L
x
Q

P
x
A

S
x
P

K
|
K

Q
x
N

G
|
G

D
x
F

L
x
F

Y
|
Y

I
x
V

L
x
I

D
|
D

R
|
R

A
|
A

T
|
T

G
|
G

E
|
E

P
x
L

L
|
L

T
x
S

C138 (= C205) modified: Disulfide link with 139
C139 (vs. gap) modified: Disulfide link with 138
R144 (= R206) binding pyrroloquinoline quinone
T189 (= T272) binding pyrroloquinoline quinone
GA 205:206 (≠ QA 292:293) binding pyrroloquinoline quinone
E207 (= E294) binding Ca(2+)
T264 (≠ N339) binding pyrroloquinoline quinone
N284 (= N359) binding Ca(2+)
D329 (= D403) binding Ca(2+)
K356 (= K426) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
415 binding substrate
419:420 binding pyrroloquinoline quinone
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
24% identity, 82% coverage: 107:711/734 of query aligns to 53:581/757 of O05542

query
sites
O05542

D

D

W

W

P

L

F

S

W

Y

G

G

G

R

D

S

A

Y

Q

S

A

E

T

Q

R

R

Y

Y

S

S

P

P

L

L

D

D

E

Q

I

I

T

N

P

T

E

E

N

N

V

V

A

G

G

K

L

L

E

K

R

L

A

A

F

W

V

H

Y

Y

R

-

T

-

G

-

D

-

M

-

P

-

S

-

A

-

E

D

A

L

D

D

G

T

K

N

Y

R

S

G

P

Q

E

E

T

G

T

T

P

P

L

L

K

I

I

V

G

N

D

G

D

V

L

M

L

Y

M

A

C

T

S

T

A

N

M

W

N

S

I

K

L

M

I

K

S

A

I

L

D

D

A

A

T

T

G

G

E

K

E

L

N

L

W

W

R

S

Y

Y

D

D

P

P

G

K

V

V

P

P

A

G

E

N

A

I

I

A

P

D

Y

R

G

G

A

C

S

C

C

-

R

-

G

-

V

-

S

-

I

-

Y

-

T

-

D

D

P

T

A

V

A

S

A

R

E

G

G

A

T

A

L

Y

C

W

A

N

T

G

R

K

V

V

I

Y

E

F

G

G

T

T

L

F

D

D

A

G

K

R

L

L

V

I

A

A

V

L

D

D

A

A

F

K

T

T

G

G

D

K

P

L

C

V

E

W

G

S

F

V

G

Y

E

-

N

-

G

-

T

T

V

I

D

P

L

K

W

E

Q

A

D

Q

I

L

G

G

E

H

R

Q

V

R

P

S

G

-

W

-

Y

Y

S

T

V

V

T

D

A

G

P

A

P

P

A

R

V

I

V

A

R

K

G

G

I

K

L

V

V

L

T

I

G

G

A

-

Q

-

V

-

K

-

D

N

G

G

Q

G

A

A

E

E

D

F

A

G

P

A

S

R

G

G

V

F

V

V

R

S

G

A

Y

F

D

D

A

A

V

E

T

T

G

G

D

K

L

L

V

D

W

W

A

R

W

F

-

F

D

T

L

V

A

P

A

N

P

P

E

E

A

N

N

K

R

P

A

D

G

G

P

A

A

S

G

D

E

D

V

I

Y

L

T

M

R

S

-

K

-

A

-

Y

-

P

-

T

-

W

-

G

-

K

-

N

-

G

-

A

-

W

-

K

-

Q

-

G

G

G

T

G

P

G

N

T

M

V

W

W

T

D

T

S

A

L

V

V

A

Y

D

D

E

P

E

V

L

T

G

D

Y

L

V

V

Y

Y

L

L

P

G

L

V

G

G

N

N

S

G

S

S

-

P

V

W

D

N

Y

Y

Y

K

G

F

S

R

N

S

R

E

S

G

E

K

A

G

E

D

N

N

A

L

F

F

A

L

T

G

S

S

L

I

V

V

A

A

L

I

D

N

A

P

T

D

T

T

G

G

E

K

E

Y

V

V

W

W

H

H

F

F

Q

Q

T

E

V

T

R

P

H

M

D

D

V

E

W

W

D

D

Y

Y

D

T

L

S

G

V

S

Q

Q

Q

P

I

T

M

L

T

L

L

D

D

W

M

-

P

-

V

-

N

-

G

Q

E

G

M

T

R

P

H

A

V

I

I

L

V

L

H

P

A

S

P

K

K

Q

N

G

G

D

F

L

F

Y

Y

I

I

L

I

D

D

R

A

A

K

-

T

-

G

-

K

-

F

-

I

T

T

G

G

E

K

P

P

L

Y

T

T

P

Y

M

E

E

N

E

W

L

A

T

N

G

G

L

L

P

D

Q

P

G

V

D

T

V

G

E

R

P

P

D

N

Y

Y

I

V

S

P

P

-

T

-

Q

-

P

-

A

-

S

-

A

-

W

-

A

-

T

-

L

-

R

-

M

-

P

-

L

-

E

-

P

-

R

-

D

-

A

-

W

-

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-

F

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T

-

A

-

I

-

D

D

Q

A

L

L

W

W

C

T

R

L

I

-

Q

-

F

-

H

-

R

-

A

-

N

-

Y

-

E

-

G

-

Y

-

F

-

T

-

P

-

S

-

S

T

D

G

R

K

P

P

W

W

I

L

Q

G

Y

I

P

P

G

G

Y

E

N

L

G

G

S

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D

N

W

F

G

A

S

A

V

M

A

A

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Y

D

S

S

P

Q

K

R

T

G

K

L

L

I

V

I

Y

A

I

N

P

Y

A

N

Q

D

Q

V

I

P

P

N

-

Y

-

N

-

R

-

L

-

V

-

P

-

R

-

D

-

E

-

V

-

E

-

S

-

V

L

P

L

I

Y

H

D

E

G

Q

Q

E

K

S

G

G

G

A

F

Q

K

A

A

G

-

E

-

N

-

A

-

E

-

G

-

A

-

D

-

P

-

Q

-

A

-

G

-

S

-

P

-

Y

Y

G

H

I

D

S

A

V

W

N

N

A

L

G

G

W

L

R

D

N

M

E

N

M

K

T

I

G

G

I

L

P

F

C

D

V

D

R

N

P

D

P

P

Y

E

G

H

H

V

I

A

R

A

A

K

I

K

D

D

L

F

E

L

T

K

G

V

E

L

T

K

V

G

W

W

D

T

R

V

P

A

F

W

G

D

T

P

A

E

R

K

R

M

N

A

G

P

P

A

F

F

G

T

I

I

P

N

S

H

Y

K

L

G

P

P

F

W

D

N

I

G

G

G

T

L

P

L

N

A

N

T

G

A

G

G

S

N

V

V

V

-

T

-

A

-

G

-

V

-

I

I

F

F

M

Q

G

G

A

L

A

A

T

N

D

G

N

E

L

-

F

F

R

H

A

A

I

Y

D

D

V

A

E

T

T

N

G

G

E

N

T

D

L

L

W

Y

Q

S

D

F

V

P

L

A

P

Q

A

S

G

A

A

I

Q

I

A

A

N

P

P

P

I

V

T

T

Y

Y

E

T

V

A

D

N

G

G

R

K

Q

Q

Y

Y

V

V

M

A

I

V

Sites not aligning to the query:

1:34 signal peptide
35 modified: Pyrrolidone carboxylic acid

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
29% identity, 46% coverage: 107:442/734 of query aligns to 21:343/684 of 1yiqA

query
sites
1yiqA

D

N

W

W

P

M

F

S

W

T

G

G

G

R

D

T

A

Y

Q

D

A

E

T

Q

R

R

Y

Y

S

S

P

P

L

L

D

K

E

Q

I

I

T

S

P

D

E

Q

N

N

V

V

A

G

G

Q

L

L

E

G

R

L

A

A

F

W

V

S

Y

Y

R

K

T

L

G

-

D

-

M

-

P

-

S

-

A

-

E

-

A

-

D

D

G

L

K

D

Y

R

S

G

P

V

E
|
E

T

A

T

T

P

P

L

I

K

V

I

V

G

D

D

G

D

V

L

M

L

Y

M

T

C

T

S

G

A

P

M

F

N

S

I

V

L

V

I

Y

S

A

I

L

D

D

A

A

A

R

T

D

G

G

E

R

E

L

N

I

W

W

R

K

Y

Y

D

D

P

P

G

-

V

-

P

Q

A

S

E

D

A

R

I

H

P

R

Y

A

G

G

A

E

S

A

C
|
C

-
x
C

-

D

-

A

-

V

-

N

R
|
R

G

G

V

V

S

A

I

V

Y

W

T

K

D

G

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R

K

V

V

I

Y

E

V

G

G

T

V

L

L

D

D

A

G

K

R

L

L

V

E

A

A

V

I

D

D

A

A

F

K

T

T

G

G

D

Q

P

R

C

A

E

W

G

-

F

-

G

-

E

-

N

-

G

-

T

S

V

V

D

D

L

T

W

R

Q

A

D

D

I

H

G

K

E

R

R

S

V

-

P

-

G

-

W

-

Y

Y

S

T

V

I

T
|
T

A

G

P

A

P

P

A

R

V

V

R

N

G

G

I

K

L

V

V

V

T

I

G

G

A

-

Q

-

V

-

K

-

D

N

G
|
G

Q
x
G

A
|
A

E
|
E

D

F

A

G

P

V

S

R

G

G

V

Y

V

V

R

T

G

A

Y

Y

D

D

A

A

V

E

T

T

G

G

D

K

L

E

V

-

W

-

A

A

W

W

D

R

L

F

A

Y

A

T

P

V

E

P

A

G

N

D

R

P

A

K

G

L

P

P

A

E

G

G

E

K

-

G

-

M

-

E

-

I

-

A

-

K

-

T

-

W

-

F

-

G

-

D

-

A

-

Y

V

V

Y

E

T

Q

R

G

G

G

T

G

P

G

N
x
T

M

A

W
|
W

T

D

T

S

A

F

V

A

A

Y

D

D

E

P

E

E

L

L

G

N

Y

L

V

L

Y

Y

L

I

P

G

L

V

G

G

N
|
N

S

G

S

S

V

L

-

W

D

D

Y

P

Y

K

G

W

S

R

N

S

R

Q

S

A

E

K

A

G

E

D

N

N

A

L

F

F

A

L

T

S

S

S

L

I

V

V

A

A

L

V

D

N

A

A

T

D

T

T

G

G

E

E

E

Y

V

V

W

W

H

H

F

Y

Q

Q

T

T

V

T

R

P

H

G

D

D

V

A

W

W

D
|
D

Y

Y

D

T

L

A

G

T

S

Q

Q

H

P

M

T

I

L

L

-

A

-

E

-

L

-

P

L

I

D

D

W

G

Q

K

G

P

T

R

P

K

A

V

I

L

L

M

L

Q

P

A

S

P

K
|
K

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

I

D

D

R

R

A

A

T

T

G

G

E

E

P

L

L

L

T

S

active site: E178 (= E294), N255 (= N359), D300 (= D403)
binding calcium ion: E178 (= E294), N255 (= N359), D300 (= D403)
binding pyrroloquinoline quinone: E63 (= E157), C109 (= C205), C110 (vs. gap), R115 (= R206), T160 (= T272), G175 (= G291), G176 (≠ Q292), A177 (= A293), E178 (= E294), T235 (≠ N339), W237 (= W341), K327 (= K426)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
binding pyrroloquinoline quinone: 390, 391, 477, 542

Q88JH0 Quinoprotein alcohol dehydrogenase PedH; Lanthanide-dependent pyrroloquinoline quinone-dependent alcohol dehydrogenase; Lanthanide-dependent PQQ-ADH; EC 1.1.2.- from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
23% identity, 86% coverage: 84:713/734 of query aligns to 25:552/595 of Q88JH0

query
sites
Q88JH0

A

A

Q

H

A

A

E

A

A

V

E

S

A

N

Q

E

A

E

L

I

V

L

E

Q

R

D

P

P

A

K

Q

N

P

P

Q

Q

P

Q

E

I

V

V

G

T

A

N

D

-

W

-

P

-

F

-

W

-

G

G

G

L

D

G

A

V

Q

Q

A

G

T

Q

R

R

Y

Y

S

S

P

P

L

L

D

D

E

L

I

L

T

N

P

V

E

N

N

N

V

V

A

K

G

E

L

L

E

R

R

P

A

V

F

W

V

A

Y

F

R

S

T

F

G

G

D

-

M

-

P

-

S

-

A

-

E

-

A

G

D

E

G

K

K

Q

Y

R

S

G

P

Q

E
x
Q

T

A

T

Q

P

P

L

L

K

I

I

K

G

D

D

G

D

V

L

M

L

Y

M

L

C

T

S

G

A

S

M

Y

N

S

I

R

L

V

I

F

S

A

I

V

D

D

A

A

A

R

T

T

G

G

E

K

N

L

W

W

R

Q

Y

Y

D

D

P

A

G

R

V

L

P

P

A

D

E

D

A

I

I

R

P

P

Y

-

G

-

A

-

S
x
C

C
|
C

-

D

-

V

-

I

-

N

R
|
R

G

G

V

V

S

A

I

L

Y

Y

T

-

D

-

P

-

A

-

E

-

G

G

T

N

L

L

C

-

A

-

T

-

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L

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W

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F

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A

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-

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N

Y

Y

L

A

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F

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D

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I

-

G

-

T

-

P

-

N

-

N
x
W

G

G

S

V

V

L

V

T

T

T

A

K

G

G

G

N

V

L

I

V

F

F

M

T

G

G

A

T

A

P

T

-

D

E

N

G

L

F

F

L

R

Q

A

A

I

F

D

N

V

A

E

K

T

T

G

G

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D

T

K

L

V

W

W

Q

E

D

F

V

Q

L

T

P

G

A

S

G

G

A

V

Q

L

A

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N

S

P

P

I

V

T

T

Y

W

E

E

V

M

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D

G

G

R

E

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Q

Y

Y

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V

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S

I

V

A

V

A

S

Q87 (≠ E157) binding pyrroloquinoline quinone
C131 (≠ S204) modified: Disulfide link with 132
C132 (= C205) modified: Disulfide link with 131
R137 (= R206) binding pyrroloquinoline quinone
S181 (≠ T272) binding pyrroloquinoline quinone
G197 (≠ Q292) binding pyrroloquinoline quinone
G198 (≠ A293) binding pyrroloquinoline quinone
E199 (= E294) binding Pr(3+)
W263 (≠ E349) binding pyrroloquinoline quinone
N281 (≠ S361) binding Pr(3+)
D323 (= D403) binding Pr(3+)
D325 (= D405) binding Pr(3+)
R350 (≠ K426) binding pyrroloquinoline quinone
F412 (≠ Y521) mutation to I: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ser-561 or Gln-561.; mutation to V: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Ala-561.
N417 (≠ D526) binding pyrroloquinoline quinone
W493 (≠ N653) binding pyrroloquinoline quinone

Sites not aligning to the query:

557 binding pyrroloquinoline quinone
561 W→A: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Val-412.; mutation W->S,Q: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ile-412.

4mh1B Crystal structure and functional studies of quinoprotein l-sorbose dehydrogenase from ketogulonicigenium vulgare y25 (see paper)
25% identity, 88% coverage: 90:734/734 of query aligns to 3:514/514 of 4mh1B

query
sites
4mh1B

A

A

Q

Q

A

T

L

A

V

I

E

T

R

D

P

E

A

M

Q

L

P

A

Q

N

P

P

E

P

V

A

G

G

A

-

D

E

W

W

P

I

F

S

W

Y

G

G

G

Q

D

N

A

Q

Q

E

A

N

T

Y

R

R

Y

H

S

S

P

P

L

L

D

T

E

Q

I

I

T

T

P

T

E

E

N

N

V

V

A

G

G

Q

L

L

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Q

R

-

A

-

F

L

V

V

Y

W

R

A

T

R

G

G

D

M

M

Q

P

P

S

-

A

-

E

-

A

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D

-

G

G

K

K

Y

V

S

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P

-

E
x
Q

T

V

T

T

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P

L

L

K

I

I

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G

D

D

G

D

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L

M

L

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M

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A

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L

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G

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W

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A

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F

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G

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|
R

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W

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|
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W

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F

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A

T

A

A

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N

-

G

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P

-

F

-

G

-

I

-

P

-

S

N

Y
|
Y

L

S

P

P

F

V

D

-

I

-

G

-

T

-

P

-

N

-

G

-

S

-

V

L

V

S

T

T

A

G

G

G

V

V

I

L

F

F

M

N

G

G

A

G

A

-

T

T

D

D

N

R

L

Y

F

F

R

R

A

A

I

L

D

S

V

Q

E

E

T

T

G

G

E

E

T

T

L

L

W

W

Q

Q

D

T

V

R

L

L

P

A

A

T

G

V

A

A

Q

S

A

G

N

Q

P

A

I

I

T

S

Y

Y

E

E

V

V

D

D

G

G

R

M

Q

Q

Y

Y

V

V

M

A

I

I

A

A

A

G

-

G

-

V

-

S

-
x
Y

-

G

-

S

-

G

-

L

-

N

-

S

-

A

-

L

V

A

G

G

H

E

H

R

F

V

M

D

E

S

T

T

G

A

V

I

G

G

D

N

W

A

M

V

I

Y

A

V

Y

F

A

A

L

L

P

P

Q

Q

active site: D240 (= D403)
binding pyrroloquinoline quinone: Q60 (≠ E157), F106 (≠ Y201), R111 (= R206), S154 (≠ T272), T169 (≠ P297), W196 (≠ A346), E242 (≠ D405), K284 (≠ E478), W346 (= W527), Y421 (= Y643), Y485 (vs. gap)

6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
25% identity, 51% coverage: 91:467/734 of query aligns to 5:369/562 of 6zcvA

query
sites
6zcvA

Q

E

A

E

L

I

V

L

E

Q

R

D

P

P

A

K

Q

N

P

P

Q

Q

P

Q

E

I

V

V

G

T

A

N

D

-

W

-

P

-

F

-

W

-

G

G

G

L

D

G

A

V

Q

Q

A

G

T

Q

R

R

Y

Y

S

S

P

P

L

L

D

D

E

L

I

L

T

N

P

V

E

N

N

N

V

V

A

K

G

E

L

L

E

R

R

P

A

V

F

W

V

A

Y

F

R

S

T

F

G

G

D

-

M

-

P

-

S

-

A

-

E

-

A

G

D

E

G

K

K

Q

Y

R

S

G

P

Q

E
x
Q

T

A

T

Q

P

P

L

L

K

I

I

K

G

D

D

G

D

V

L

M

L

Y

M

L

C

T

S

G

A

S

M

Y

N

S

I

R

L

V

I

F

S

A

I

V

D

D

A

A

A

R

T

T

G

G

E

K

N

L

W

W

R

Q

Y

Y

D

D

P

A

G

R

V

L

P

P

A

D

E

D

A

I

I

R

P

P

Y

-

G

-

A

-

S
x
C

C
|
C

-

D

-

V

-
x
I

-

N

R
|
R

G

G

V

V

S

A

I

L

Y

Y

T

-

D

-

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

G

T

N

R

L

V

V

I

F

E

F

G

G

T

T

L

L

D

D

A

A

K

K

L

L

V

V

A

A

V

L

D

N

A

K

F

-

T

-

G

-

D

-

P

-

C

-

E

-

G

-

F

-

G

-

E

D

N

T

G

G

T

K

V

V

D

V

L

W

W

S

Q

K

D

K

I

V

G

A

E

D

R

H

V

K

P

E

G

G

W

-

Y

Y

S

S

V

I

T
x
S

A

A

P

A

P

P

A

M

V

I

V

V

R
x
N

G

G

I
x
K

L

L

V

I

T

T

G

G

A

V

Q

-

V

-

K

-

D

-

G

A

Q
x
G

A
x
G

E
|
E

D

F

A

G

P

V

S

V

G

G

V

K

V

I

R

Q

G

A

Y

Y

D

N

A

P

V

E

T

N

G

G

D

E

L

L

V

L

W

W

-

M

-

R

-

P

-

T

-

V

-

E

-

G

-

H

-

M

A

G

W

Y

D

V

L

Y

A

K

A

D

P

G

E

K

A

A

N

I

R

E

A

N

G

G

P

I

P

S

A

G

G

G

E

E

V

A

Y

G

T

K

R

T

G

W

T

P

P

G

N

D

M

L

W

W

T

K

T

T

A

G

V

G

A

A

D

A

E

P

E
x
W

L

L

G

G

Y

G

V

Y

Y

Y

L

D

P

P

-

E

-

T

-

N

-

L

-

I

-

L

L

F

G

G

N

T

S

G

S
x
N

V

P

D

A

Y

P

Y

W

G

N

S

S

N

H

R

L

S

R

E

P

A

G

E

D

N

N

A

L

F

Y

A

S

T

S

S

S

L

R

V

L

A

A

L

L

D

N

A
x
P

T
x
D

T

D

G

G

E

T

E

I

V

K

W

W

H

H

F

F

Q

Q

T

S

V

T

R

P

H

H

D

D

V

G

W

W

D
|
D

Y

F

D
|
D

L

G

G

V

S

N

Q

E

P

L

T

I

L

S

L

F

D

N

W

Y

Q

K

G

D

-

G

-

K

-

E

T

V

P

K

A

A

I

A

L

A

L

T

P

A

S

D

K
x
R

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

L

D

D

R

R

A

T

T

N

G

G

E

K

P

F

L

I

T

R

-

G

-

F

-

P

-

F

-

V

-

D

P

K

M

I

E

T

E

W

L

A

T

T

G

G

L

L

P

D

Q

K

G

-

D

D

V

G

E

R

P

P

D

I

Y

Y

I

N

S

D

P

A

T

S

Q

R

P

P

A

G

S

A

active site: E172 (= E294), N254 (≠ S361), D296 (= D403)
binding calcium ion: N161 (≠ R279), K163 (≠ I281), P278 (≠ A385), D279 (≠ T386)
binding pyrroloquinoline quinone: Q60 (≠ E157), C104 (≠ S204), C105 (= C205), I108 (vs. gap), R110 (= R206), S154 (≠ T272), G170 (≠ Q292), G171 (≠ A293), E172 (= E294), W236 (≠ E349), D298 (= D405), R323 (≠ K426)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 390, 466, 529, 530

A0A3F2YLY8 Lanthanide-dependent methanol dehydrogenase XoxF; Lanthanide-dependent MDH; Ln(3+)-dependent MDH; EC 1.1.2.10 from Methylotuvimicrobium buryatense (Methylomicrobium buryatense) (see paper)
27% identity, 51% coverage: 68:441/734 of query aligns to 11:369/617 of A0A3F2YLY8

query
sites
A0A3F2YLY8

A

A

S

S

P

T

E

V

A

A

G

A

T

L

E

L

A

A

A

V

P

P

A

A

G

-

T

-

S

-

V

V

A

S

Q

Q

A

A

E

N

A

S

E

E

A

V

Q

E

A

K

L

L

V

T

E

K

R

N

P

P

A

A

Q

-

P

-

Q

-

P

-

E

-

V

-

G

-

A

-

D

N

W

W

P

A

F

T

W

W

G

G

D

N

A

Y

Q

H

A

G

T

T

R

R

Y

Y

S

S

P

E

L

L

D

K

E

Q

I

I

T

N

P

T

E

S

N

N

V

V

A

K

G

N

L

L

E

Q

R

P

A

A

F

W

V

T

Y

F

R

S

T

T

G

G

D

V

M

L

P

R

S

G

A

H

E

E

A

G

D

G

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

V

G

N

D

D

D

V

L

M

L

Y

M

I

C

H

S

T

A

P

M

F

-

P

N

N

I

T

L

V

I

Y

S

A

I

I

D

D

A

Q

A

K

T

T

G

Q

E

A

E

V

N

I

W

W

R

E

Y

Y

D

T

P

P

G

Q

V

Q

P

D

A

A

E

D

A

V

I

T

P

I

Y

P

G

V

A

M

S
x
C

C
|
C

R

D

G

T

V

V

S

N

I

-

Y

-

T
x
R

D

G

P

L

A

A

A

Y

A

G

E

D

G

G

T

-

L

-

C

-

A

-

T

-

R

K

V

I

I

F

E

L

G

Q

T

Q

L

S

D

D

A

T

K

V

L

L

V

T

A

A

V

L

D

D

A

A

F

K

T

T

G

G

D

K

P

R

C

V

E

W

G

S

F

V

G

-

E

Q

N

N

G

G

T

D

V

P

D

K

L

L

W

G

Q

M

D

-

I

-

G

-

E

-

R

-

V

-

P

-

G

-

W

-

Y

-

S

T

V

N

T
|
T

A

Q

P

A

P

P

A

L

V

V

R

K

G

D

I

K

L

V

V

I

T

T

G

G

A

I

Q
x
S

V

-

K

-

D

-

G

-

Q
x
G

A
x
G

E
|
E

D

F

A

G

P

V

S

R

G

G

V

F

V

L

R

A

G

A

Y

Y

D

N

A

I

V

R

T

T

G

G

D

E

L

L

V

D

W

W

A

K

W

G

D

Y

L

S

A

M

A

G

P

P

E

D

A

A

N

D

R

T

A

L

G

I

P

N

P

P

A

T

-

K

-

T

-

W

-

K

-

D

-

G

-

K

-

V

-

Q

-

P

-

V

-

G

-

K

-

D

-

S

-

T

-

S

-

T

-

W

-

E

G

G

E

D

V

Q

Y

W

T

K

R

I

G

G

T

G

P

G

N
x
T

M

T

W
|
W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

E

L

L

G

N

Y

L

V

V

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

S

P

S

S

V

T

D

-

Y

-

Y

W

G

N

S

P

N

A

R

Q

S

R

E

P

A

G

E

D

N

N

A

K

F

W

A

S

T

M

S

S

L

L

V

W

A

A

L

R

D

N

A

A

T

D

T

T

G

G

E

E

E

V

V

K

W

W

H

V

F

Y

Q

Q

T

M

V

T

R

P

H

H

D

D

V

E

W

W

D
|
D

Y

Y

D
|
D

L

G

G

I

S

N

Q

E

P

V

T

A

L

L

L

V

D

D

W

Q

-

E

-

I

-

K

-

G

Q

Q

G

M

T

R

P

K

A

T

I

A

L

V

L

H

P

F

S

D

K
x
R

Q

N

G

G

D

F

L

G

Y

Y

I

T

L

L

D

D

R

R

A

V

T

T

G

G

E

E

P

L

L

L

C129 (≠ S204) modified: Disulfide link with 130
C130 (= C205) modified: Disulfide link with 129
R135 (≠ T212) binding pyrroloquinoline quinone
T179 (= T272) binding pyrroloquinoline quinone
S194 (≠ Q287) binding pyrroloquinoline quinone
G195 (≠ Q292) binding pyrroloquinoline quinone
G196 (≠ A293) binding pyrroloquinoline quinone
E197 (= E294) binding La(3+)
T265 (≠ N339) binding pyrroloquinoline quinone
W267 (= W341) binding pyrroloquinoline quinone
N285 (= N359) binding La(3+)
D327 (= D403) binding La(3+)
D329 (= D405) binding La(3+)
R354 (≠ K426) binding pyrroloquinoline quinone

Sites not aligning to the query:

412 modified: Disulfide link with 441
420 binding pyrroloquinoline quinone
441 modified: Disulfide link with 412
506 binding pyrroloquinoline quinone
570 binding pyrroloquinoline quinone

7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 46% coverage: 112:448/734 of query aligns to 18:363/588 of 7o6zB

query
sites
7o6zB

G

G

G

K

D

D

A

Y

Q

A

A

N

T

T

R

R

Y

H

S

S

P

P

L

L

D

K

E

Q

I

I

T

N

P

T

E

Q

N

N

V

V

A

K

G

G

L

L

E

H

R

M

A

A

F

W

V

S

Y

F

R

S

T

T

G

G

D

V

M

L

P

R

S

G

A

H

E
|
E

A

G

D

Q

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

I

G

G

D

D

D

R

L

M

L

Y

M

V

C

V

S

T

A

P

M

Y

-

P

N

N

I

I

L

V

I

W

S

A

I

L

D

D

A

I

A

S

T

K

G

G

-

N

-

S

-

Y

E

E

E

V

N

L

W

W

R

K

Y

Y

D

A

P

P

G

R

V

Q

P

D

A

D

E

K

A

A

I

V

P

S

Y

-

G

T

A

A

S
x
C

C
|
C

R

D

G

T

V

V

S

N

I

-

Y

-

T
x
R

D

G

P

A

A

S

A

Y

A

A

E

D

G

G

T

-

L

-

C

-

A

-

T

-

R

K

V

I

I

V

E

F

G

N

T

T

L

L

D

D

A

G

K

Y

L

V

V

V

A

C

V

L

D

D

A

A

F

N

T

T

G

G

D

K

P

-

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

-

D

E

L

L

W

W

Q

K

D

T

I

K

G

F

E

A

R

D

V

V

P

N

G

K

W

G

Y

E

S

T

V

S

T
|
T

A

P

P

A

P

P

A

I

V

I

V

V

R

K

G

D

I

K

L

V

V

V

T

T

G

G

A

Y

Q

-

V

-

K

-

D

-

G
|
G

Q
x
G

A
x
D

E
|
E

D

F

A

G

P

A

S

R

G

G

V

R

V

F

R

A

G

A

Y

F

D

D

A

L

V

N

T

S

G

G

D

K

L

M

V

V

W

W

A

Q

-

A

-

Y

-

S

-

N

-

G

-

P

-

D

-

S

-

D

-

V

-

L

-

G

-

P

-

D

W

F

D

N

L

S

A

K

A

H

P

P

E

E

A

Y

N

G

R

Q

A

A

G

G

P

Q

P

D

A

L

G

G

-

V

-

K

-

T

-

Y

-

P

-

D

E

E

V

E

Y

W

T

K

R

R

G

G

T

G

P

G

N

C

M

A

W
|
W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

K

L

L

G

D

Y

L

V

I

Y

Y

L

Y

P

N

L

T

G

G

N
|
N

S

P

S

G

V

L

D

-

Y
x
W

Y

S

G

P

S

S

N

Y

R

R

S

T

E

E

A

A

-

K

-

T

-

H

-

E

-

A

-

N

-

E

-

P

-

W

-

K

-

W

E

D

N

N

A

K

F

W

A

S

T

M

S

T

L

I

V

F

A

A

L

R

D

K

A

P

T

D

T

T

G

G

E

E

E

A

V

V

W

W

H

G

F

Y

Q

Q

T

M

V

T

R

P

H

F

D

D

V

Q

W

W

D
|
D

Y

Y

D
|
D

L

G

G

I

S

N

Q

E

P

D

T

V

L

L

L

V

D

D

W

I

-

T

-

V

-

D

-

G

Q

S

G

K

T

K

P

P

A

C

I

L

L

V

L

H

P

F

S

D

K
x
R

Q

N

G

G

D

F

L

C

Y

Y

I

V

L

L

D

N

R

R

A

T

T

D

G

G

E

T

P

I

L

I

T

R

P

A

M

N

E

K

E

F

L

V

T

T

binding methanol: E173 (= E294), W263 (≠ Y364), D314 (= D403)
binding Neodymium Ion: E173 (= E294), N259 (= N359), D314 (= D403), D316 (= D405)
binding pyrroloquinoline quinone: E55 (= E149), C105 (≠ S204), C106 (= C205), R111 (≠ T212), T155 (= T272), G170 (= G291), G171 (≠ Q292), D172 (≠ A293), E173 (= E294), W241 (= W341), D316 (= D405), R341 (≠ K426)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 403, 481, 544, 545

7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 46% coverage: 112:448/734 of query aligns to 18:363/588 of 7o6zA

query
sites
7o6zA

G

G

G

K

D

D

A

Y

Q

A

A

N

T

T

R

R

Y

H

S

S

P

P

L

L

D

K

E

Q

I

I

T

N

P

T

E

Q

N

N

V

V

A

K

G

G

L

L

E

H

R

M

A

A

F

W

V

S

Y

F

R

S

T

T

G

G

D

V

M

L

P

R

S

G

A

H

E

E

A

G

D

Q

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

I

G

G

D

D

D

R

L

M

L

Y

M

V

C

V

S

T

A

P

M

Y

-

P

N

N

I

I

L

V

I

W

S

A

I

L

D

D

A

I

A

S

T

K

G

G

-

N

-

S

-

Y

E

E

E

V

N

L

W

W

R

K

Y

Y

D

A

P

P

G

R

V

Q

P

D

A

D

E

K

A

A

I

V

P

S

Y

-

G

T

A

A

S

C

C

C

R

D

G

T

V

V

S

N

I

-

Y

-

T

R

D

G

P

A

A

S

A

Y

A

A

E

D

G

G

T

-

L

-

C

-

A

-

T

-

R

K

V

I

I

V

E

F

G

N

T

T

L

L

D

D

A

G

K

Y

L

V

V

V

A

C

V

L

D

D

A

A

F

N

T

T

G

G

D

K

P

-

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

-

D

E

L

L

W

W

Q

K

D

T

I

K

G

F

E

A

R

D

V

V

P

N

G

K

W

G

Y

E

S

T

V

S

T

T

A

P

P

A

P

P

A

I

V

I

V

V

R

K

G

D

I

K

L

V

V

V

T

T

G

G

A

Y

Q

-

V

-

K

-

D

-

G

G

Q

G

A

D

E
|
E

D

F

A

G

P

A

S

R

G

G

V

R

V

F

R

A

G

A

Y

F

D

D

A

L

V

N

T

S

G

G

D

K

L

M

V

V

W

W

A

Q

-

A

-

Y

-

S

-

N

-

G

-

P

-

D

-

S

-

D

-

V

-

L

-

G

-

P

-

D

W

F

D

N

L

S

A

K

A

H

P

P

E

E

A

Y

N

G

R

Q

A

A

G

G

P

Q

P

D

A

L

G

G

-

V

-

K

-

T

-

Y

-

P

-

D

E

E

V

E

Y

W

T

K

R

R

G

G

T

G

P

G

N

C

M

A

W

W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

K

L

L

G

D

Y

L

V

I

Y

Y

L

Y

P

N

L

T

G

G

N
|
N

S

P

S

G

V

L

D

-

Y
x
W

Y

S

G

P

S

S

N

Y

R

R

S

T

E

E

A

A

-

K

-

T

-

H

-

E

-

A

-

N

-

E

-

P

-

W

-

K

-

W

E

D

N

N

A

K

F

W

A

S

T

M

S

T

L

I

V

F

A

A

L

R

D

K

A

P

T

D

T

T

G

G

E

E

E

A

V

V

W

W

H

G

F

Y

Q

Q

T

M

V

T

R

P

H

F

D

D

V

Q

W

W

D
|
D

Y

Y

D
|
D

L

G

G

I

S

N

Q

E

P

D

T

V

L

L

L

V

D

D

W

I

-

T

-

V

-

D

-

G

Q

S

G

K

T

K

P

P

A

C

I

L

L

V

L

H

P

F

S

D

K

R

Q

N

G

G

D

F

L

C

Y

Y

I

V

L

L

D

N

R

R

A

T

T

D

G

G

E

T

P

I

L

I

T

R

P

A

M

N

E

K

E

F

L

V

T

T

binding methanol: E173 (= E294), W263 (≠ Y364), D314 (= D403)
binding Neodymium Ion: E173 (= E294), N259 (= N359), D314 (= D403), D316 (= D405)

Sites not aligning to the query:

binding methanol: 545

6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
26% identity, 48% coverage: 87:441/734 of query aligns to 1:343/563 of 6damA

query
sites
6damA

E

N

A

S

E

E

A

V

Q

E

A

K

L

L

V

T

E

K

R

N

P

P

A

A

Q

-

P

-

Q

-

P

-

E

-

V

-

G

-

A

-

D

N

W

W

P

A

F

T

W

W

G

G

D

N

A

Y

Q

H

A

G

T

T

R

R

Y

Y

S

S

P

E

L

L

D

K

E

Q

I

I

T

N

P

T

E

S

N

N

V

V

A

K

G

N

L

L

E

Q

R

P

A

A

F

W

V

T

Y

F

R

S

T

T

G

G

D

V

M

L

P

R

S

G

A

H

E
|
E

A

G

D

G

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

V

G

N

D

D

D

V

L

M

L

Y

M

I

C

H

S

T

A

P

M

F

-

P

N

N

I

T

L

V

I

Y

S

A

I

I

D

D

A

Q

A

K

T

T

G

Q

E

A

E

V

N

I

W

W

R

E

Y

Y

D

T

P

P

G

Q

V

Q

P

D

A

A

E

D

A

V

I

T

P

I

Y

P

G

V

A

M

S
x
C

C
|
C

R

D

G

T

V

V

S

N

I

-

Y

-

T
x
R

D

G

P

L

A

A

A

Y

A

G

E

D

G

G

T

-

L

-

C

-

A

-

T

-

R

K

V

I

I

F

E

L

G

Q

T

Q

L

S

D

D

A

T

K

V

L

L

V

T

A

A

V

L

D

D

A

A

F

K

T

T

G

G

D

K

P

R

C

V

E

W

G

S

F

V

G

-

E

Q

N

N

G

G

T

D

V

P

D

K

L

L

W

G

Q

M

D

-

I

-

G

-

E

-

R

-

V

-

P

-

G

-

W

-

Y

-

S

T

V

N

T
|
T

A

Q

P

A

P

P

A

L

V

V

R

K

G

D

I

K

L

V

V

I

T

T

G

G

A

I

Q
x
S

V

-

K

-

D

-

G

-

Q
x
G

A
x
G

E
|
E

D

F

A

G

P

V

S

R

G

G

V

F

V

L

R

A

G

A

Y

Y

D

N

A

I

V

R

T

T

G

G

D

E

L

L

V

D

W

W

A

K

W

G

D

Y

L

S

A

M

A

G

P

P

E

D

A

A

N

D

R

T

A

L

G

I

P

N

P

P

A

T

-

K

-

T

-

W

-

K

-

D

-

G

-

K

-

V

-

Q

-

P

-

V

-

G

-

K

-

D

-

S

-

T

-

S

-

T

-

W

-

E

G

G

E

D

V

Q

Y

W

T

K

R

I

G

G

T

G

P

G

N
x
T

M

T

W
|
W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

E

L

L

G

N

Y

L

V

V

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

S

P

S

S

V

T

D

-

Y

-

Y

W

G

N

S

P

N

A

R

Q

S

R

E

P

A

G

E

D

N

N

A

K

F

W

A

S

T

M

S

S

L

L

V

W

A

A

L

R

D

N

A

A

T

D

T

T

G

G

E

E

E

V

V

K

W

W

H

V

F

Y

Q

Q

T

M

V

T

R

P

H

H

D

D

V

E

W

W

D
|
D

Y

Y

D
|
D

L

G

G

I

S

N

Q

E

P

V

T

A

L

L

L

V

D

D

W

Q

-

E

-

I

-

K

-

G

Q

Q

G

M

T

R

P

K

A

T

I

A

L

V

L

H

P

F

S

D

K
x
R

Q

N

G

G

D

F

L

G

Y

Y

I

T

L

L

D

D

R

R

A

V

T

T

G

G

E

E

P

L

L

L

active site: E171 (= E294), N259 (= N359), D301 (= D403)
binding pyrroloquinoline quinone: E55 (= E149), C103 (≠ S204), C104 (= C205), R109 (≠ T212), T153 (= T272), S168 (≠ Q287), G169 (≠ Q292), G170 (≠ A293), E171 (= E294), T239 (≠ N339), W241 (= W341), D303 (= D405), R328 (≠ K426)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 394, 480, 543, 544

I0JWN7 Lanthanide-dependent methanol dehydrogenase; Lanthanide-dependent MDH; Ln(3+)-dependent MDH; Ln-MDH; EC 1.1.2.10 from Methylacidiphilum fumariolicum (strain SolV) (see 2 papers)
25% identity, 49% coverage: 82:441/734 of query aligns to 30:375/611 of I0JWN7

query
sites
I0JWN7

S

S

V

L

A

A

Q

K

A

A

E

N

A

D

E

E

A

L

Q

I

A

K

L

L

V

E

E

K

R

E

P

P

A

G

Q

Q

P

-

Q

-

P

-

E

-

V

-

G

-

A

-

D

-

W

W

P

V

F

M

W

Q

G

N

G

K

D

N

A

Y

Q

A

A

N

T

T

R

R

Y

Y

S

S

P

E

L

L

D

N

E

Q

I

I

T

N

P

T

E

K

N

N

V

V

A

S

G

R

L

L

E

R

R

L

A

A

F

W

V

S

Y

F

R

S

T

T

G

G

D

A

M

L

P

R

S

G

A

H

E

E

A

G

D

G

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

V

G

G

D

T

L

M

L

Y

M

V

C

H

S

S

A

A

M

Y

-

P

N

N

I

H

L

V

I

Y

S

A

I

L

D

D

A

L

A

T

T

Q

G

K

-

P

-

Y

E

A

E

I

N

K

W

W

R

Q

Y

Y

D

T

P

P

G

V

V

Q

P

N

A

S

E

Q

A

A

I

V

P

A

Y

V

G

-

A

A

S
x
C

C
|
C

R

D

G

V

V

V

S

N

I

-

Y

-

T
x
R

D

G

P

L

A

A

A

Y

A

A

E

N

G

G

T

K

L

I

C

F

A

M

T

T

R

-

V

-

I

-

E

-

G

-

T

T

L

L

D

D

A

G

K

Q

L

I

V

I

A

A

V

L

D

D

A

A

F

N

T

T

G

G

D

K

P

-

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

-

D

E

L

L

W

W

Q

K

D

M

I

K

G

H

E

A

R

D

V

V

P

T

G

K

W

G

Y

E

S

T

V

I

T
|
T

A

G

P

A

P

P

A

L

V

V

R

K

G

D

I

K

L

V

V

L

T

V

G

G

A

V

Q
x
S

V

-

K

-

D

-

G

-

Q
x
G

A
x
G

E
|
E

D

F

A

G

P

V

S

R

G

G

V

R

V

V

R

G

G

A

Y

Y

D

D

A

I

V

N

T

T

G

G

D

N

L

R

V

V

W

W

A

L

W

A

D

Y

L

S

A

Q

A

G

P

P

E

D

A

E

-

E

-

V

-

L

-

D

-

S

-

D

-

F

N

N

R

K

A

E

G

F

P

P

P

Q

A

H

G

G

-

G

-

P

-

G

-

D

-

G

-

T

-

K

-

T

-

W

-

P

-

G

E

E

V

Q

Y

W

T

K

R

L

G

G

T

G

P

G

N
x
T

M

T

W
|
W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

A

L

L

G

D

Y

L

V

F

Y

Y

L

Y

P

G

L

T

G

S

N
|
N

S

P

S

G

V

T

D

-

Y

W

Y

N

G

A

S

E

N

Q

R

R

S

K

E

G

A

G

E

D

N

N

A

K

F

W

A

S

T

C

S

T

L

I

V

F

A

A

L

R

D

R

A

P

T

D

T

T

G

G

E

K

E

A

V

R

W

W

H

A

F

Y

Q

Q

T

M

V

T

R

P

H

W

D

D

V

S

W

W

D
|
D

Y

Y

D
|
D

L

G

G

V

S

N

Q

E

P

M

T

I

L

L

L

P

D

D

W

L

-

T

-

V

-

K

-

G

Q

K

G

K

T

T

P

P

A

C

I

L

L

V

L

H

P

F

S

D

K
x
R

Q

N

G

G

D

F

L

G

Y

Y

I

V

L

L

D

D

R

R

A

R

T

T

G

G

E

Q

P

L

L

I

C138 (≠ S204) modified: Disulfide link with 139
C139 (= C205) modified: Disulfide link with 138
R144 (≠ T212) binding pyrroloquinoline quinone
T188 (= T272) binding pyrroloquinoline quinone
S203 (≠ Q287) binding pyrroloquinoline quinone
G204 (≠ Q292) binding pyrroloquinoline quinone
G205 (≠ A293) binding pyrroloquinoline quinone
E206 (= E294) binding Ce(3+); binding Eu(3+)
T270 (≠ N339) binding pyrroloquinoline quinone
W272 (= W341) binding pyrroloquinoline quinone
N290 (= N359) binding Ce(3+); binding Eu(3+)
D333 (= D403) binding Ce(3+); binding Eu(3+)
D335 (= D405) binding Ce(3+); binding Eu(3+)
R360 (≠ K426) binding pyrroloquinoline quinone

Sites not aligning to the query:

414 modified: Disulfide link with 443
443 modified: Disulfide link with 414
501 binding pyrroloquinoline quinone
566 binding pyrroloquinoline quinone

1flgA Crystal structure of the quinoprotein ethanol dehydrogenase from pseudomonas aeruginosa (see paper)
23% identity, 45% coverage: 111:439/734 of query aligns to 21:357/582 of 1flgA

query
sites
1flgA

W

Y

G

G

G

M

D

G

A

T

Q

H

A

A

T

Q

R

R

Y

W

S

S

P

P

L

L

D

K

E

Q

I

V

T

N

P

A

E

D

N

N

V

V

A

F

G

K

L

L

E

T

R

P

A

A

F

W

V

S

Y

Y

R

S

T

F

G

G

D

D

M

E

P

-

S

-

A

-

E

-

A

-

D

-

G

-

K

K

Y

Q

S

R

P

G

E

Q

T
x
E

T

S

P

Q

L

A

K

I

I

V

G

S

D

D

D

G

L

V

L

I

M

Y

C

V

S

T

A

A

-

S

M

Y

N

S

I

R

L

L

I

F

S

A

I

L

D

D

A

A

A

K

T

T

G

G

E

K

E

R

N

L

W

W

R

T

Y

Y

D

N

P

H

G

R

V

L

P

P

A

D

E

D

A

I

I

R

P

P

Y

-

G

-

A

-

S
x
C

C
|
C

-

D

-

V

-

N

R
|
R

G

G

V

A

S

A

I

I

Y

Y

T

G

D

D

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R

K

V

V

I

F

E

F

G

G

T

T

L

L

D

D

A

A

K

S

L

V

V

V

A

A

V

L

D

N

A

K

F

N

T

T

G

G

D

K

P

V

C

V

-

W

-

K

E

K

G

K

F

F

G

A

E

D

N

H

G

G

T

A

V

G

D

-

L

-

W

-

Q

-

D

-

I

-

G

-

E

-

R

-

V

-

P

-

G

-

W

-

Y

Y

S

T

V

M

T
|
T

A

G

P

A

P

P

A

T

V

I

V

V

R

K

G

D

I

G

L

K

V

T

T

G

G

K

A

V

Q

L

V

L

K

I

D

H

G

G

Q

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A
x
S

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x
G

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|
D

A
x
E

P

F

S

G

G

V

V

V

V

G

R

R

G

L

Y

F

-

A

-

R

D

D

A

P

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D

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T

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G

D

E

L

E

V

I

W

W

-

M

-

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-

P

-

F

-

V

-

E

-

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-

H

-

M

-

G

-

R

-

L

-

N

-

G

-

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-

D

-

S

-

T

-

V

A

T

W

G

D

D

L

V

A

K

A

A

P

P

E

S

-

W

A

P

N

D

R

D

A

R

G

N

P

S

P

P

A

T

G

G

E

K

V

V

-

E

-

S

Y

W

T

S

R

H

G

G

T

G

P

G

N

A

M

P

W
|
W

T

Q

T

S

A

A

V

S

A

F

D

D

E

A

E

E

L

T

G

N

Y

T

V

I

Y

I

L

V

P

G

L

A

G

G

N
|
N

S

P

-

G

-

P

-

W

-

N

-

T

-

W

S

A

V

R

D

T

Y

A

Y

K

G

G

S

G

N

N

R

P

S

H

E

D

A

Y

E

D

N

S

A

L

F

Y

A

T

T

S

S

G

L

Q

V

V

A

G

L

V

D

D

A

P

T

S

G

G

E

E

E

V

V

K

W

W

H

F

F

Y

Q

Q

T

H

V

T

R

P

H

N

D

D

V

A

W

W

D
|
D

Y

F

D

S

L

G

G

N

S

N

Q

E

P

L

T

V

L

L

L

F

D

D

W

Y

Q

K

G

A

T

K

P

D

A

G

I

K

L

I

L

V

P

K

S

A

-

T

-

A

-

H

-

A

-

D

K
x
R

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

V

D

D

R

R

A

S

T

N

G

G

E

K

active site: E179 (≠ A296), N266 (= N359), D316 (= D403)
binding calcium ion: E179 (≠ A296), N266 (= N359), D316 (= D403)
binding pyrroloquinoline quinone: E61 (≠ T158), C105 (≠ S204), C106 (= C205), R111 (= R206), T155 (= T272), S176 (≠ A293), G177 (≠ E294), D178 (= D295), W248 (= W341), R344 (≠ K426)

Sites not aligning to the query:

binding calcium ion: 11, 14, 17
binding pyrroloquinoline quinone: 413, 414, 489, 552, 553

Q9Z4J7 Quinoprotein ethanol dehydrogenase; QEDH; Quinoprotein alcohol dehydrogenase (cytochrome c); Quinoprotein alcohol dehydrogenase (cytochrome c550); EC 1.1.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
23% identity, 45% coverage: 111:439/734 of query aligns to 55:391/623 of Q9Z4J7

query
sites
Q9Z4J7

W

Y

G

G

G

M

D

G

A

T

Q

H

A

A

T

Q

R

R

Y

W

S

S

P

P

L

L

D

K

E

Q

I

V

T

N

P

A

E

D

N

N

V

V

A

F

G

K

L

L

E

T

R

P

A

A

F

W

V

S

Y

Y

R

S

T

F

G

G

D

D

M

-

P

-

S

-

A

-

E

-

A

-

D

E

G

K

K

Q

Y

R

S

G

P

Q

E
|
E

T

S

T

Q

P

A

L

I

K

V

I

S

G

D

D

G

D

V

L

I

L

Y

M

V

C

T

S

A

A

S

M

Y

N

S

I

R

L

L

I

F

S

A

I

L

D

D

A

A

A

K

T

T

G

G

E

K

E

R

N

L

W

W

R

T

Y

Y

D

N

P

H

G

R

V

L

P

P

A

D

E

D

A

I

I

R

P

P

Y

-

G

-

A

-

S
x
C

C
|
C

-

D

-

V

-

N

R
|
R

G

G

V

A

S

A

I

I

Y

Y

T

G

D

D

P

-

A

-

E

-

G

-

T

-

L

-

C

-

A

-

T

-

R

K

V

V

I

F

E

F

G

G

T

T

L

L

D

D

A

A

K

S

L

V

V

V

A

A

V

L

D

N

A

K

F

N

T

T

G

G

D

K

P

V

C

V

-

W

-

K

E

K

G

K

F

F

G

A

E

D

N

H

G

G

T

A

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G

D

-

L

-

W

-

Q

-

D

-

I

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W

-

Y

Y

S

T

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M

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|
T

A

G

P

A

P

P

A

T

V

I

V

V

R

K

G

D

I

G

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K

V

T

T

G

G

K

A

V

Q

L

V

L

K

I

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x
H

G
|
G

Q
x
S

A

S

E

G

D

D

A
x
E

P

F

S

G

G

V

V

V

V

G

R

R

G

L

Y

F

-

A

-

R

D

D

A

P

V

D

T

T

G

G

D

E

L

E

V

I

W

W

-

M

-

R

-

P

-

F

-

V

-

E

-

G

-

H

-

M

-

G

-

R

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L

-

N

-

G

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-

D

-

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V

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W

G

D

D

L

V

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A

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D

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R

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P

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T

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G

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K

V

V

-

E

-

S

Y

W

T

S

R

H

G

G

T

G

P

G

N

A

M

P

W

W

T

Q

T

S

A

A

V

S

A

F

D

D

E

A

E

E

L

T

G

N

Y

T

V

I

Y

I

L

V

P

G

L

A

G

G

N
|
N

S

P

-

G

-

P

-

W

-

N

-

T

-

W

S

A

V

R

D

T

Y

A

Y

K

G

G

S

G

N

N

R

P

S

H

E

D

A

Y

E

D

N

S

A

L

F

Y

A

T

T

S

S

G

L

Q

V

V

A

G

L

V

D

D

A

P

T

S

G

G

E

E

E

V

V

K

W

W

H

F

F

Y

Q

Q

T

H

V

T

R

P

H

N

D

D

V

A

W

W

D
|
D

Y

F

D

S

L

G

G

N

S

N

Q

E

P

L

T

V

L

L

L

F

D

D

W

Y

Q

K

G

A

T

K

P

D

A

G

I

K

L

I

L

V

P

K

S

A

-

T

-

A

-

H

-

A

-

D

K
x
R

Q

N

G

G

D

F

L

F

Y

Y

I

V

L

V

D

D

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R

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T

N

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K

E95 (= E157) binding pyrroloquinoline quinone
C139 (≠ S204) modified: Disulfide link with 140
CC 139:140 (≠ SC 204:205) mutation to AA: 15-fold decrease in catalytic activity with the natural electron acceptor cytochrome c550. Does not affect, or even increases, catalytic activity with artificial electron acceptors. Shows high decreased affinity for primary alcohols, while the affinity for the secondary alcohol 2-propanol is unaltered.
C140 (= C205) modified: Disulfide link with 139
R145 (= R206) binding pyrroloquinoline quinone
T189 (= T272) binding pyrroloquinoline quinone
HGS 207:209 (≠ DGQ 290:292) binding pyrroloquinoline quinone
E213 (≠ A296) binding Ca(2+)
N300 (= N359) binding Ca(2+)
D350 (= D403) binding Ca(2+)
R378 (≠ K426) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:34 signal peptide
45 binding Ca(2+)
48 binding Ca(2+)
51 binding Ca(2+)
523 binding pyrroloquinoline quinone
587 binding pyrroloquinoline quinone

6fkwA Europium-containing methanol dehydrogenase (see paper)
26% identity, 44% coverage: 118:441/734 of query aligns to 24:341/576 of 6fkwA

query
sites
6fkwA

T

T

R

R

Y

Y

S

S

P

E

L

L

D

N

E

Q

I

I

T

N

P

T

E

K

N

N

V

V

A

S

G

R

L

L

E

R

R

L

A

A

F

W

V

S

Y

F

R

S

T

T

G

G

D

A

M

L

P

R

S

G

A

H

E
|
E

A

G

D

G

G

-

K

-

Y

-

S

-

P

-

E

-

T

-

P

P

L

L

K

V

I

V

G

G

D

T

L

M

L

Y

M

V

C

H

S

S

A

A

M

Y

-

P

N

N

I

H

L

V

I

Y

S

A

I

L

D

D

A

L

A

T

T

Q

G

K

-

P

-

Y

E

A

E

I

N

K

W

W

R

Q

Y

Y

D

T

P

P

G

V

V

Q

P

N

A

S

E

Q

A

A

I

V

P

A

Y

V

G

-

A

A

S
x
C

C
|
C

R

D

G

V

V

V

S

N

I

-

Y

-

T
x
R

D

G

P

L

A

A

A

Y

A

A

E

N

G

G

T

K

L

I

C

F

A

M

T

T

R

-

V

-

I

-

E

-

G

-

T

T

L

L

D

D

A

G

K

Q

L

I

V

I

A

A

V

L

D

D

A

A

F

N

T

T

G

G

D

K

P

-

C

-

E

-

G

-

F

-

G

-

E

-

N

-

G

-

T

-

V

-

D

E

L

L

W

W

Q

K

D

M

I

K

G

H

E

A

R

D

V

V

P

T

G

K

W

G

Y

E

S

T

V

I

T
|
T

A

G

P

A

P

P

A

L

V

V

R

K

G

D

I

K

L

V

V

L

T

V

G

G

A

V

Q
x
S

V

-

K

-

D

-

G

-

Q
x
G

A
x
G

E
|
E

D

F

A

G

P

V

S

R

G

G

V

R

V

V

R

G

G

A

Y

Y

D

D

A

I

V

N

T

T

G

G

D

N

L

R

V

V

W

W

A

L

W

A

D

Y

L

S

A

Q

A

G

P

P

E

D

A

E

-

E

-

V

-

L

-

D

-

S

-

D

-

F

N

N

R

K

A

E

G

F

P

P

P

Q

A

H

G

G

-

G

-

P

-

G

-

D

-

G

-

T

-

K

-

T

-

W

-

P

-

G

E

E

V

Q

Y

W

T

K

R

L

G

G

T

G

P

G

N
x
T

M

T

W
|
W

T

G

T

W

A

Y

V

S

A

Y

D

D

E

P

E

A

L

L

G

D

Y

L

V

F

Y

Y

L

Y

P

G

L

T

G

S

N
|
N

S

P

S

G

V

T

D

-

Y

W

Y

N

G

A

S

E

N

Q

R

R

S

K

E

G

A

G

E

D

N

N

A

K

F

W

A

S

T

C

S

T

L

I

V

F

A

A

L

R

D

R

A

P

T

D

T

T

G

G

E

K

E

A

V

R

W

W

H

A

F

Y

Q

Q

T

M

V

T

R

P

H

W

D

D

V

S

W

W

D
|
D

Y

Y

D
|
D

L

G

G

V

S

N

Q

E

P

M

T

I

L

L

L

P

D

D

W

L

-

T

-

V

-

K

-

G

Q

K

G

K

T

T

P

P

A

C

I

L

L

V

L

H

P

F

S

D

K
x
R

Q

N

G

G

D

F

L

G

Y

Y

I

V

L

L

D

D

R

R

A

R

T

T

G

G

E

Q

P

L

L

I

active site: E172 (= E294), N256 (= N359), D299 (= D403), D301 (= D405)
binding europium ion: E172 (= E294), N256 (= N359), D299 (= D403), D301 (= D405)
binding pyrroloquinoline quinone: E55 (= E149), C104 (≠ S204), C105 (= C205), R110 (≠ T212), T154 (= T272), S169 (≠ Q287), G170 (≠ Q292), G171 (≠ A293), E172 (= E294), T236 (≠ N339), W238 (= W341), D301 (= D405), R326 (≠ K426)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 388, 467, 531, 532

Query Sequence

>WP_069333494.1 NCBI__GCF_003046325.1:WP_069333494.1
MPIHPLRSLPLLALIAATPLAAQVQPAQGLPEADVAPQQAEPSPDAQPGGAGDAPQGQTE
QAAPEGAASPEAGTEAAPAGTSVAQAEAEAQALVERPAQPQPEVGADWPFWGGDAQATRY
SPLDEITPENVAGLERAFVYRTGDMPSAEADGKYSPETTPLKIGDDLLMCSAMNILISID
AATGEENWRYDPGVPAEAIPYGASCRGVSIYTDPAAAEGTLCATRVIEGTLDAKLVAVDA
FTGDPCEGFGENGTVDLWQDIGERVPGWYSVTAPPAVVRGILVTGAQVKDGQAEDAPSGV
VRGYDAVTGDLVWAWDLAAPEANRAGPPAGEVYTRGTPNMWTTAVADEELGYVYLPLGNS
SVDYYGSNRSEAENAFATSLVALDATTGEEVWHFQTVRHDVWDYDLGSQPTLLDWQGTPA
ILLPSKQGDLYILDRATGEPLTPMEELTGLPQGDVEPDYISPTQPASAWATLRMPPLEPR
DAWGFTAIDQLWCRIQFHRANYEGYFTPPSSDRPWIQYPGYNGGSDWGSVAVDSQRGLII
ANYNDVPNYNRLVPRDEVESVPIHEQESGAQAGENAEGAADPQAGSPYGISVNAGWRNEM
TGIPCVRPPYGHIRAIDLETGETVWDRPFGTARRNGPFGIPSYLPFDIGTPNNGGSVVTA
GGVIFMGAATDNLFRAIDVETGETLWQDVLPAGAQANPITYEVDGRQYVMIAAVGHHFME
TGVGDWMIAYALPQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory