SitesBLAST

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
27% identity, 80% coverage: 160:780/781 of query aligns to 38:577/690 of Q8GR64

query
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Q8GR64

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Q

E81 (≠ Q211) binding pyrroloquinoline quinone
C127 (= C259) modified: Disulfide link with 128
C128 (vs. gap) modified: Disulfide link with 127
R133 (= R260) binding pyrroloquinoline quinone
T177 (= T326) binding pyrroloquinoline quinone
GA 193:194 (≠ FS 346:347) binding pyrroloquinoline quinone
E195 (≠ V348) binding Ca(2+)
T252 (≠ S395) binding pyrroloquinoline quinone
N272 (= N414) binding Ca(2+)
D317 (= D458) binding Ca(2+)
K344 (= K485) binding pyrroloquinoline quinone
NW 404:405 (= NW 583:584) binding pyrroloquinoline quinone
V547 (≠ S761) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:22 signal peptide
23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
613 binding covalent
616 binding covalent
617 binding axial binding residue
655 binding axial binding residue

O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
26% identity, 77% coverage: 159:757/781 of query aligns to 51:584/757 of O05542

query
sites
O05542

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Y

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E

G

N

G

W

L

A

P

N

E

G

E

L

S

D

P

P

A

V

P

T

T

G

Q

R

P

P

T

-

S

-

A

-

L

-

S

N

F

Y

K

V

P

P

A

D

A

A

L

L

R

-

E

-

K

-

D

-

M

-

W

W

G

T

V

L

T

T

I

G

Y

K

D

P

Q

W

L

L

A

G

C

I

R

-

I

-

Q

-

Y

-

H

-

R

-

L

-

N

-

Y

-

E

-

G

-

R

-

Y

-

T

-

P

-

S

-

L

-

N

-

G

-

T

-

I

-

V

-

Y

-

P

P

G

G

N

E

F

L

G

G

T

G

F

H

N

N

W

F

G

A

S

A

V

M

A

A

V

Y

D

S

P

P

E

K

R

T

Q

K

V

L

M

V

F

Y

G

-

M

-

P

-

T

-

Y

-

L

-

P

-

F

-

T

-

V

-

E

-

L

-

V

I

P

P

A

A

E

Q

D

Q

I

I

P

P

P

L

G

Y

A

D

N

G

E

Q

T

K

A

G

S

G

E

F

Q

K

G

A

L

Y

N

H

R

D

N

A

E

W

G

N

A

-

P

-

Y

-

G

-

V

-

I

-

M

L

G

G

P

L

F

D

L

M

G

N

P

K

L

I

G

G

V

L

P

F

C

D

S

D

A

N

P

D

P

P

W

E

G

H

Y

V

V

A

A

A

G

K

A

K

D

D

L

F

R

L

T

K

G

-

D

-

I

-

A

-

Y

-

M

V

H

L

R

K

N

G

G

W

T

T

V

V

-

A

-

W

-

D

-

P

R

E

D

K

M

M

T

A

P

P

L

-

P

A

L

F

P

T

L

I

K

N

V

H

G

K

V

G

P

P

G

W

I

N

G

G

P

L

I

L

V

A

T

T

R

A

G

G

G

N

V

V

A

I

F

F

L

Q

G

G

A

L

A

A

V

N

D

G

D

E

Y

F

L

-

R

H

A

A

Y

Y

D

D

V

A

T

T

T

N

G

G

E

N

Q

D

L

L

W

Y

Q

S

A

F

R

P

L

A

P

Q

A

S

G

A

G

I

Q

I

S

A

T

P

P

P

M

V

T

T

Y

Y

E

T

Q

A

D

N

G

G

R

K

Q

Q

F

Y

V

V

V

A

I

V

V

E

A

V

G

G

Sites not aligning to the query:

1:34 signal peptide
35 modified: Pyrrolidone carboxylic acid

8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
26% identity, 77% coverage: 159:757/781 of query aligns to 17:550/723 of 8gy2A

query
sites
8gy2A

P

P

G

G

E

D

W

W

H

L

Q

S

Y

Y

G

G

R

R

T

S

Q

Y

Y

S

G

E

Q

Q

R

R

Y

Y

S

S

P

P

L

L

E

D

Q

Q

I

I

N

N

I

T

Q

E

N

N

V

V

A

G

E

K

L

L

E

K

Q

L

V

A

W

W

Q

H

Y

Y

Q

D

T

L

G

D

D

-

V

-

K

-

L

-

P

-

Q

-

D

-

V

-

T

T

E

N

T

R

T

G

Y

Q

Q

E

V

G

T

T

P

P

L

L

K

I

V

V

D

N

D

G

R

V

L

M

Y

Y

I

A

C

T

T

T

P

N

H

W

N

S

L

K

A

M

I

K

A

A

L

L

D

D

A

A

T

T

G

G

K

K

E

L

A

L

W

W

R

S

F

Y

D

D

A

P

N

K

S

V

-

P

G

G

L

N

E

I

P

A

D
|
D

R

R

Q

G

-
x
C

H

D

Q

T

T

V

C

S

R

R

G

G

V

A

T

A

Y

Y

W

W

R

N

D

G

P

-

A

-

R

-

A

-

E

-

G

-

E

-

L

-

C

-

A

-

E

-

R

K

V

V

Y

Y

L

F

P

G

T

T

A

F

D

D

A

G

R

R

L

L

I

I

A

A

L

L

D

D

A

A

R

K

S

T

G

G

A

K

-

L

V

V

C

W

T

S

F

V

F

Y

A

T

D

I

E

P

G

K

T

E

L

A

H

Q

L

L

E

G

S

H

G

Q

M

R

P

S

Y

Y

T

T

P

V

E
x
D

G

G

F

-

Y

-

S

-

T

-

S

-

P

A

P

P

V

R

A

I

V

A

G

K

G

G

R

K

I

V

I

L

I

I

G

G

G

-

A

-

V

-

N

-

D

N

N

G

F
x
G

S
x
A

V
x
E

F

F

S

G

Q

A

S

R

G

G

V

F

I

V

R

S

A

A

F

F

D

D

A

A

N

E

T

T

G

G

A

K

L

L

L

D

W

W

N

R

W

F

D

F

S

T

-

V

-

P

-

N

-

P

-

E

G

N

N

K

P

P

D

D

E

G

T

A

A

A

P

S

I

D

D

D

W

I

M

L

N

M

G

S

E

K

T

A

Y

Y

T

P

A

T

N

W

S

G

P

K

N

N

S

G

-

A

-

W

-

K

-

Q

-

G

-

T

-

V

W
|
W

S

D

V

S

F

L

S

V

V

Y

D

D

E

P

E

V

R

T

G

D

L

L

V

V

Y

Y

I

L

P

G

L

V

G

G

N
|
N

Q

G

V

S

P

P

D

W

Q

N

L

Y

G

K

F

F

D

-

R

R

S

S

P

E

A

G

V

K

E

G

E

D

H

N

-

L

-

F

S

L

S

G

S

S

V

I

V

V

A

A

L

I

D

N

V

P

T

D

T

T

G

G

Q

K

K

Y

A

V

W

W

V

H

F

F

Q

Q

T

E

V

T

H

P

H

M

D

D

L

E

W

W

D
|
D

M

Y

D

T

V

S

P

V

A

Q

Q

Q

P

I

V

M

L

T

I

L

D

D

L

M

D

P

I

V

D

N

G

G

Q

E

T

M

V

R

P

H

A

V

L

I

V

V

Q

H

P

A

T

P

K
|
K

Q

N

G

G

D

F

I

F

Y

Y

V

I

L

I

N

D

R

A

E

K

T

T

G

G

E

K

P

F

I

I

L

-

P

-

V

-

T

T

E

G

E

K

P

P

A

Y

P

T

Q

Y

E

E

G

N

G

W

L

A

P

N

E

G

E

L

S

D

P

P

A

V

P

T

T

G

Q

R

P

P

T

-

S

-

A

-

L

-

S

N

F

Y

K

V

P

P

A

D

A

A

L

L

R

-

E

-

K

-

D

-

M

-

W

W

G

T

V

L

T

T

I

G

Y

K

D

P

Q

W

L

L

A

G

C

I

R

-

I

-

Q

-

Y

-

H

-

R

-

L

-

N

-

Y

-

E

-

G

-

R

-

Y

-

T

-

P

-

S

-

L

-

N

-

G

-

T

-

I

-

V

-

Y

-

P

P

G

G

N

E

F

L

G

G

T

G

F

H

N

N

W
x
F

G

A

S

A

V

M

A

A

V

Y

D

S

P

P

E

K

R

T

Q

K

V

L

M

V

F

Y

G

-

M

-

P

-

T

-

Y

-

L

-

P

-

F

-

T

-

V

-

E

-

L

-

V

I

P

P

A

A

E

Q

D

Q

I

I

P

P

P

L

G

Y

A

D

N

G

E

Q

T

K

A

G

S

G

E

F

Q

K

G

A

L

Y

N

H

R

D

N

-

E

-

G

-

A

-

P

-

Y

-

G

A

V

W

I

N

M

L

G

G

P

L

F

D

L

M

G

N

P

K

L

I

G

G

V

L

P

F

C

D

S

D

A

N

P

D

P

P

W

E

G

H

Y

V

V

A

A

A

G

K

A

K

D

D

-

F

-

L

-

K

-

V

L

L

R

K

T

G

G

W

D

T

I

V

A

A

Y

W

M

-

H

-

R

-

N

-

G

-

T

D

V

P

R

E

D

K

M

M

T

A

P

P

L

-

P

A

L

F

P

T

L

I

K

N

V

H

G

K

V

G

P

P

G
x
W

I

N

G

G

P

L

I

L

V

A

T

T

R

A

G

G

G

N

V

V

A

I

F

F

L

Q

G

G

A

L

A

A

V

N

D

G

D

E

Y

F

L

-

R

H

A

A

Y

Y

D

D

V

A

T

T

T

N

G

G

E

N

Q

D

L

L

W

Y

Q

S

A

F

R

P

L

A

P

Q

A

S

G

A

G

I

Q

I

S

A

T

P

P

P

M

V

T

T

Y

Y

E

T

Q

A

D

N

G

G

R

K

Q

Q

F

Y

V

V

V

A

I

V

V

E

A

V

G

G

binding calcium ion: E181 (≠ V348), N263 (= N414), D308 (= D458)
binding heme c: D104 (= D253)
binding pyrroloquinoline quinone: C107 (vs. gap), C108 (vs. gap), D163 (≠ E320), G179 (≠ F346), A180 (≠ S347), E181 (≠ V348), W245 (= W396), N263 (= N414), D308 (= D458), K335 (= K485), F398 (≠ W584), W489 (≠ G695)

Sites not aligning to the query:

binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
31% identity, 49% coverage: 117:498/781 of query aligns to 8:369/718 of Q4W6G0

query
sites
Q4W6G0

A
x
S

L
|
L

P
|
P

L
|
L

L
x
K

A
x
S

A
x
L

I
x
A

G
x
V

V
x
A

A
x
V

I
x
L

W
x
L

S
|
S

W
x
L

T
x
A

G
|
G

D
x
T

E
x
P

A
|
A

G

-

H
x
L

A
|
A

G
x
A

S
x
D

L
x
I

P
|
P

T
x
A

E
x
N

V
|
V

V
x
D

S
x
G

A
|
A

E
x
R

P
x
I

D

-

L
x
I

G
x
A

P
x
A

T
x
D

A
x
K

P
x
E

P
|
P

G
|
G

E
x
N

W
|
W

H
x
M

Q
x
S

Y
x
T

G
|
G

R
|
R

T
|
T

Q
x
Y

Y
x
D

G
x
E

Q
|
Q

R
|
R

Y
|
Y

S
|
S

P
|
P

L
|
L

E
x
K

Q
|
Q

I
|
I

N
x
S

I
x
D

Q
|
Q

N
|
N

V
|
V

A
x
G

E
x
Q

L
|
L

E
x
G

Q
x
L

V
x
A

W
|
W

Q
x
S

Y
|
Y

Q
x
K

T

-

G

-

D

-

V
x
L

K
x
D

L
|
L

P
x
D

Q
x
R

D
x
G

V
|
V

T

-

E

-

T

-

Y

-

Q
x
E

V
x
A

T
|
T

P
|
P

L
x
I

K
x
V

V
|
V

D
|
D

D
x
G

R
x
A

L
x
M

Y
|
Y

I
x
T

C
x
T

T
x
G

P
|
P

H
x
F

N
x
S

L
x
V

A
x
V

I
x
Y

A
|
A

L
|
L

D
|
D

A
|
A

A
x
R

T
x
D

G
|
G

K
x
R

E
x
L

A
x
I

W
|
W

R
x
K

F
x
Y

D
|
D

A
x
P

N
x
Q

S
|
S

G

-

L

-

E
x
D

P
x
R

D
x
H

R
|
R

Q
x
A

H
x
G

Q
x
E

T
x
A

C
|
C

-
x
C

-
x
D

-
x
A

-
x
V

-
x
N

R
|
R

G
|
G

V
|
V

T
x
A

Y
x
V

W
|
W

R
x
K

D
x
G

P

-

A

-

R

-

A

-

E

-

G

-

E

-

L

-

C

-

A

-

E

-

R
x
K

V
|
V

Y
|
Y

L
x
V

P
x
G

T
x
V

A
x
L

D
|
D

A
x
G

R
|
R

L
|
L

I
x
E

A
|
A

L
x
I

D
|
D

A
|
A

R
x
K

S
x
T

G
|
G

A
x
Q

V
x
R

C
x
A

T

-

F
x
W

F
x
S

A
x
V

D
|
D

E
x
T

G
x
R

T
x
A

L
x
D

H
|
H

L
x
K

E
x
R

S
|
S

G

-

M

-

P

-

Y

-

T

-

P

-

E

-

G

-

F

-

Y
|
Y

Y
x
T

S
x
I

T
|
T

S
x
G

P
x
A

P
|
P

V
x
R

A
x
V

V
|
V

G
x
N

G
|
G

R
x
K

I
x
V

I
|
I

G
|
G

G

-

A

-

V

-

N

-

D
x
N

N
x
G

F
x
G

S
x
A

V
x
E

F
|
F

S
x
G

Q
x
V

S
x
R

G
|
G

V
x
Y

I
x
V

R
x
T

A
|
A

F
x
Y

D
|
D

A
|
A

N
x
E

T
|
T

G
|
G

A
x
K

L
x
E

L
x
A

W
|
W

N
x
R

W
x
F

D
x
Y

S
x
T

-
x
V

-
x
P

G
|
G

N
x
D

P
|
P

D
x
K

-
x
L

-
x
P

-
x
E

-
x
G

-
x
K

-
x
G

-
x
M

E
|
E

T
x
I

A
|
A

P
x
A

I
x
K

D
x
T

W
|
W

M
x
F

N

-

G
|
G

E
x
D

T
x
A

Y
|
Y

T
x
V

A
x
E

N
x
Q

S
x
G

P
x
G

-
x
G

N
x
T

S
x
A

W
|
W

S
x
D

V
x
S

F
|
F

S
x
A

V
x
Y

D
|
D

E
x
P

E
|
E

R
x
L

G
x
N

L
|
L

V
x
L

Y
|
Y

I
|
I

P
x
G

L
x
V

G
|
G

N
|
N

-
x
G

Q
x
S

V
x
L

P
x
W

D
|
D

Q
x
P

L
x
K

G
x
W

F
x
R

D
x
S

R
x
Q

S
x
A

P
x
K

A
x
G

V
x
D

E
x
N

E
x
L

H
x
F

S
x
L

S
|
S

V
x
I

V
|
V

A
|
A

L
x
V

D
x
N

V
x
A

T
x
D

T
|
T

G
|
G

Q
x
E

K
x
Y

A
x
V

W
|
W

V
x
H

F
x
Y

Q
|
Q

T
|
T

V
x
T

H
x
P

H
x
G

D
|
D

L
x
A

W
|
W

D
|
D

M
x
Y

D
x
T

V
x
A

P
x
T

A
x
Q

Q
x
H

P
x
M

V
x
I

L
|
L

I
x
A

D
x
E

L
|
L

D
x
P

I
|
I

D
|
D

G
|
G

Q
x
K

T
x
P

V
x
R

P
x
K

A
x
V

L
|
L

V
x
M

Q
|
Q

P
x
A

T
x
P

K
|
K

Q
x
N

G
|
G

D
x
F

I
x
F

Y
|
Y

V
|
V

L
x
I

N
x
D

R
|
R

E
x
A

T
|
T

G
|
G

E
|
E

C138 (= C259) modified: Disulfide link with 139
C139 (vs. gap) modified: Disulfide link with 138
R144 (= R260) binding pyrroloquinoline quinone
T189 (= T326) binding pyrroloquinoline quinone
GA 205:206 (≠ FS 346:347) binding pyrroloquinoline quinone
E207 (≠ V348) binding Ca(2+)
T264 (≠ N394) binding pyrroloquinoline quinone
N284 (= N414) binding Ca(2+)
D329 (= D458) binding Ca(2+)
K356 (= K485) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
415 binding substrate
419:420 binding pyrroloquinoline quinone
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
32% identity, 44% coverage: 159:498/781 of query aligns to 19:340/684 of 1yiqA

query
sites
1yiqA

P

P

G

G

E

N

W

W

H

M

Q

S

Y

T

G

G

R

R

T

T

Q

Y

Y

D

G

E

Q

Q

R

R

Y

Y

S

S

P

P

L

L

E

K

Q

Q

I

I

N

S

I

D

Q

Q

N

N

V

V

A

G

E

Q

L

L

E

G

Q

L

V

A

W

W

Q

S

Y

Y

Q

K

T

-

G

-

D

-

V

L

K

D

L

L

P

D

Q

R

D

G

V

V

T

-

E

-

T

-

Y

-

Q
x
E

V

A

T

T

P

P

L

I

K

V

V

V

D

D

D

G

R

V

L

M

Y

Y

I

T

C

T

T

G

P

P

H

F

N

S

L

V

A

V

I

Y

A

A

L

L

D

D

A

A

A

R

T

D

G

G

K

R

E

L

A

I

W

W

R

K

F

Y

D

D

A

P

N

Q

S

S

G

-

L

-

E

D

P

R

D

H

R

R

Q

A

H

G

Q

E

T

A

C
|
C

-
x
C

-

D

-

A

-

V

-

N

R
|
R

G

G

V

V

T

A

Y

V

W

W

R

K

D

G

P

-

A

-

R

-

A

-

E

-

G

-

E

-

L

-

C

-

A

-

E

-

R

K

V

V

Y

Y

L

V

P

G

T

V

A

L

D

D

A

G

R

R

L

L

I

E

A

A

L

I

D

D

A

A

R

K

S

T

G

G

A

Q

V

R

C

A

T

-

F

W

F

S

A

V

D

D

E

T

G

R

T

A

L

D

H

H

L

K

E

R

S

S

G

-

M

-

P

-

Y

-

T

-

P

-

E

-

G

-

F

-

Y

Y

Y

T

S

I

T
|
T

S

G

P

A

P

P

V

R

A

V

V

V

G

N

G

G

R

K

I

V

I

I

G

G

G

-

A

-

V

-

N

-

D

N

N
x
G

F
x
G

S
x
A

V
x
E

F

F

S

G

Q

V

S

R

G

G

V

Y

I

V

R

T

A

A

F

Y

D

D

A

A

N

E

T

T

G

G

A

K

L

E

L

A

W

W

N

R

W

F

D

Y

S

T

-

V

-

P

G

G

N

D

P

P

D

K

-

L

-

P

-

E

-

G

-

K

-

G

-

M

E

E

T

I

A

A

P

A

I

K

D

T

W

W

M

F

N

-

G

G

E

D

T

A

Y

Y

T

V

A

E

N

Q

S

G

P

G

-

G

N
x
T

S

A

W
|
W

S

D

V

S

F

F

S

A

V

Y

D

D

E

P

E

E

R

L

G

N

L

L

V

L

Y

Y

I

I

P

G

L

V

G

G

N
|
N

-

G

Q

S

V

L

P

W

D

D

Q

P

L

K

G

W

F

R

D

S

R

Q

S

A

P

K

A

G

V

D

E

N

E

L

H

F

S

L

S

S

V

I

V

V

A

A

L

V

D

N

V

A

T

D

T

T

G

G

Q

E

K

Y

A

V

W

W

V

H

F

Y

Q

Q

T

T

V

T

H

P

H

G

D

D

L

A

W

W

D
|
D

M

Y

D

T

V

A

P

T

A

Q

Q

H

P

M

V

I

L

L

I

A

D

E

L

L

D

P

I

I

D

D

G

G

Q

K

T

P

V

R

P

K

A

V

L

L

V

M

Q

Q

P

A

T

P

K
|
K

Q

N

G

G

D

F

I

F

Y

Y

V

V

L

I

N

D

R

R

E

A

T

T

G

G

E

E

active site: E178 (≠ V348), N255 (= N414), D300 (= D458)
binding calcium ion: E178 (≠ V348), N255 (= N414), D300 (= D458)
binding pyrroloquinoline quinone: E63 (≠ Q211), C109 (= C259), C110 (vs. gap), R115 (= R260), T160 (= T326), G175 (≠ N345), G176 (≠ F346), A177 (≠ S347), E178 (≠ V348), T235 (≠ N394), W237 (= W396), K327 (= K485)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
binding pyrroloquinoline quinone: 390, 391, 477, 542

C5AXV8 Lanthanide-dependent ethanol dehydrogenase; Lanthanide-dependent EtDH; Ln-dependent EtDH; Lanthanide-dependent formaldehyde dehydrogenase; PQQ-dependent ethanol dehydrogenase; EC 1.1.2.-; EC 1.2.2.- from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) (see paper)
26% identity, 85% coverage: 99:762/781 of query aligns to 3:555/587 of C5AXV8

query
sites
C5AXV8

M

M

R

R

R

N

Q

H

L

F

L

L

T

T

E

A

D

V

G

G

H

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A

A

A

P

L

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S

L

V

P

G

L

L

A

A

L

L

P

P

L

A

L

I

A

A

A

E

I

V

G

-

V

-

A

-

I

-

W

-

S

-

W

-

T

T

G

E

D

Q

E

D

A

I

G

L

H

N

A

D

G

A

S

K

L

T

P

T

T

D

E

D

V

V

S

T

A

Y

E

-

P

-

D

G

L

L

G

G

P

P

T

Q

A

A

P

-

G

-

E

-

W

-

H

-

Q

-

Y

-

G

-

R

-

T

-

Q

-

Y

-

G

-

Q

Q

R

R

Y

F

S

S

P

P

L

I

E

K

Q

T

I

L

N

N

I

R

Q

D

N

N

V

I

A

K

E

G

L

L

E

V

Q

P

V

A

W

W

Q

S

Y

F

Q

S

T

F

G

G

D

G

V

E

K

K

L

-

P

-

Q

-

D

-

V

-

T

-

E

Q

T

R

T

G

Y

Q

Q

E

V

S

T

Q

P

A

L

L

K

V

V

K

D

D

D

G

R

V

L

L

Y

Y

I

V

C

T

T

G

P

S

H

Y

N

S

L

R

A

L

I

Y

A

A

L

V

D

D

A

A

A

R

T

T

G

G

K

E

E

K

A

K

W

W

R

E

F

Y

D

N

A

A

N

R

S

L

G

-

L

-

E

-

P

P

D

E

R

G

Q

I

H

L

Q

P

T

C

C

C

R

-

G

-

V

-

T

-

Y

-

W

-

R

-

D

D

P

V

A

V

R

N

A

R

E

G

G

A

E

A

L

L

C

Y

A

K

E

D

R

K

V

V

Y

Y

L

F

P

G

T

T

A

L

D

D

A

A

R

K

L

L

I

V

A

A

L

L

D

D

A

Q

R

K

S

T

G

G

A

K

V

V

C

V

T

W

F

-

A

-

D

R

E

K

G

D

T

I

L

D

H

D

L

F

E

K

S

S

G

G

M

Y

P

S

Y

Y

T

T

P

-

E

-

G

-

F

-

Y

-

S

-

T

-

S

A

P

A

P

P

V

M

A

I

V

V

G

K

G

G

R

K

I

I

I

T

G

G

G

V

A

S

V

G

N

G

D

E

N

-

F

-

S

-

V

-

F

F

S

G

Q

V

S

V

G

G

V

R

I

V

R

E

A

A

F

R

D

D

A

A

N

E

T

T

G

G

A

E

L

V

W

W

N

K

W

R

D

P

-

V

-

I

-

E

-

G

-

H

-

M

-

G

-

E

-

L

-

N

-

G

-

K

-

P

-

S

-

T

-

M

S

T

G

G

N

K

P

A

D

N

E

E

T

S

A

W

P

P

I

-

D

-

W

-

M

-

N

-

G

G

E

D

T

T

Y

W

T

K

A

F

N

G

S

G

P

G

N

A

S

T

W

W

S

L

V

G

F

G

S

T

V

Y

D

D

E

P

E

E

R

L

G

N

L

L

V

I

Y

Y

I

F

P

G

L

T

G

G

N

N

Q

P

V

G

P

P

D

W

Q

N

L

S

G

A

F

L

D

-

R

-

S

R

P

P

A

G

V

D

E

N

E

K

H

W

S

S

S

A

S

S

V

R

V

L

A

A

L

I

D

N

V

P

T

D

T

N

G

G

Q

E

K

I

A

V

W

W

V

G

F

F

Q

Q

T

T

V

T

H

P

H

H

D

D

L

G

W

W

D

D

M

Y

D
|
D

V

G

P

V

A

N

Q

E

P

F

V

V

L

P

I

F

D

D

L

M

D

Q

I

K

D

D

G

G

Q

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T

T

V

V

P

K

A

A

L

G

V

A

Q

T

P

A

T

D

K

R

Q

N

G

G

D

F

I

F

Y

Y

V

V

L

L

N

D

R

R

E

T

T

N

G

G

E

A

P

F

I

L

L

-

P

-

V

-

T

-

E

-

P

-

A

-

P

-

Q

-

E

-

G

-

L

-

P

-

E

-

S

-

P

-

A

S

P

A

T

T

Q

P

P

F

T

V

S

S

A

N

L

I

S

N

F

W

K

A

P

K

A

G

A

I

L

-

R

-

E

-

K

-

D

D

M

E

W

N

G

G

V

R

T

P

I

I

Y

Y

D

D

Q

D

L

-

A

A

C

N

R

R

I

P

Q

G

Y

D

H

P

R

A

L

K

N

S

Y

E

E

D

G

G

R

K

Y

-

T

-

P

-

S

-

L

-

N

K

G

G

T

K

I

S

V

V

Y

Y

-

A

-

T

P

P

G

G

N

F

F

L

G

G

T

G

F

K

N

N

W

W

G

N

S

P

V

I

A

A

V

Y

D

S

P

P

E

D

R

T

Q

R

V

L

M

F

F

Y

G

-

M

-

P

-

T

-

Y

-

L

-

P

-

F

-

T

-

V

-

E

-

L

-

V

V

P

P

A

S

E

N

D

E

I

W

P

G

P

M

P

D

G

I

A

W

N

N

E

E

T

P

A

-

S

-

E

-

Q

-

G

-

L

V

N

N

R

Y

N

K

E

K

G

G

A

A

P

A

Y

Y

G

-

V

-

I

-

M

L

G

G

P

A

F

-

L

-

G

G

P

F

L

T

G

I

V

K

P

P

C

T

S

F

A

E

P

D

P

H

W

I

G

G

Y

S

V

L

A

K

G

A

A

I

D

D

L

P

R

A

T

T

G

K

D

K

I

V

A

V

Y

W

M

E

H

Y

R

K

N

N

G

-

T

-

V

-

R

-

D

-

M

-

T

-

P

-

L

-

P

K

L

A

P

P

L

L

K

W

V

A

G

G

V

V

P

-

G

-

I

-

G

-

P

-

I

L

V

T

T

T

R

A

G

G

G

N

V

L

A

V

F

F

L

T

G

G

A

T

A

P

V

-

D

E

D

G

Y

F

L

L

R

K

A

A

Y

F

D

D

V

A

T

K

T

T

G

G

E

E

Q

E

L

V

W

W

Q

K

A

F

R

Q

L

V

P

G

A

T

G

G

G

V

Q

V

S

A

T

S

P

P

M

I

T

T

Y

W

E

E

Q

Q

D

D

G

G

R

E

Q

Q

F

W

V

I

V

G

I

V

V

A

A

A

G

G

G

W

H

G

G

G

S

A

V

V

D319 (= D460) mutation to S: Loss of efficient ethanol oxidation with La(3+).

7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
25% identity, 78% coverage: 153:763/781 of query aligns to 5:547/588 of 7o6zB

query
sites
7o6zB

L

L

G

K

P

L

T

T

A

E

P

N

P

P

G

K

E

N

W

W

H

A

Q

A

Y

P

G

G

R

K

T

D

Q

Y

Y

A

G

N

Q

T

R

R

Y

H

S

S

P

P

L

L

E

K

Q

Q

I

I

N

N

I

T

Q

Q

N

N

V

V

A

K

E

G

L

L

E

H

Q

M

V

A

W

W

Q

S

Y

F

Q

S

T

T

G

G

D

V

V

L

K

R

L

-

P

-

Q

-

D

-

V

-

T

-

E

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G

Y

H

Q
x
E

V

G

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P

P

L

L

K

V

V

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D

G

D

D

R

R

L

M

Y

Y

I

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C

V

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P

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N

N

L

I

A

V

I

W

A

A

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L

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D

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G

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V

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N

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G

G

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T

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x
G

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x
G

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x
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D
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V

I

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G

G

Q

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K

A

A

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W

W

V

G

F

Y

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Q

T

M

V

T

H

P

H

F

D

D

L

Q

W

W

D
|
D

M

Y

D
|
D

V

G

P

I

A

N

Q

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P

D

V

V

L

L

I

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D

D

L

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I

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D

G

G

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S

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K

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K

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P

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L

L

V

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x
R

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G

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C

Y

Y

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V

L

L

N

N

R

R

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L

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N

H

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N

N

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G

A

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P

V

Y

Y

G

-

V

-

I

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M

-

G

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P

V

F

F

L

A

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N

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L

L

G

M

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K

P

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C

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A

P

P

G

P

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W

M

G

G

Y

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F

A

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G

A

A

F

D

D

L

V

R

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T

T

G

G

D

K

I

A

A

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Y

W

M

-

H

-

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N

-

G

-

T

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V

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D

-

M

-

T

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-

L

-

P

D

L

I

P

P

L

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K

R

V

-

G

-

V

F

P

P

G

T

I
x
W

G

S

G

G

P

A

I

L

V

V

T

T

R

D

G

G

V

L

A

A

F

F

L

Y

G

G

A

-

A

T

V

L

D

D

D

G

Y

W

L

F

R

K

A

A

Y

V

D

D

V

R

T

K

T

T

G

G

E

K

Q

V

L

L

W

W

Q

Q

A

Q

R

K

L

L

P

G

A

S

G

G

G

I

Q

I

S

G

T

N

P

P

M

I

T

S

Y

Y

E

E

Q

V

D

G

G

G

R

K

Q

Q

F

Y

V

I

V

S

I

V

V

L

A

S

G

G

G

I

H

G

G
|
G

S
x
W

V

I

G

G

binding methanol: E173 (≠ D344), W263 (≠ D418), D314 (= D458)
binding Neodymium Ion: E173 (≠ D344), N259 (= N414), D314 (= D458), D316 (= D460)
binding pyrroloquinoline quinone: E55 (≠ Q211), C105 (≠ R260), C106 (≠ G261), R111 (= R266), T155 (= T318), G170 (≠ A341), G171 (≠ V342), D172 (≠ N343), E173 (≠ D344), W241 (= W396), D316 (= D460), R341 (≠ K485), D403 (≠ N583), W481 (≠ I696), G544 (= G760), W545 (≠ S761)

7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
25% identity, 78% coverage: 153:763/781 of query aligns to 5:547/588 of 7o6zA

query
sites
7o6zA

L

L

G

K

P

L

T

T

A

E

P

N

P

P

G

K

E

N

W

W

H

A

Q

A

Y

P

G

G

R

K

T

D

Q

Y

Y

A

G

N

Q

T

R

R

Y

H

S

S

P

P

L

L

E

K

Q

Q

I

I

N

N

I

T

Q

Q

N

N

V

V

A

K

E

G

L

L

E

H

Q

M

V

A

W

W

Q

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Y

F

Q

S

T

T

G

G

D

V

V

L

K

R

L

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P

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Q

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D

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Y

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L

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V

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D

G

D

D

R

R

L

M

Y

Y

I

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C

V

T

T

P

P

H

Y

-

P

N

N

L

I

A

V

I

W

A

A

L

L

D

D

A

I

A

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T

K

G

G

K

N

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Y

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A

L

W

W

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F

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D

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N

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A

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D

D

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K

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G

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F

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W

W

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W

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K

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L

G

D

L

L

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Y

Y

I

Y

P

N

L

T

G

G

N
|
N

Q

P

V

G

P

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x
W

Q

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L

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F

Y

D

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H

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W

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D

L

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|
D

M

Y

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|
D

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L

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G

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L

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W

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L

L

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G

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A

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L

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N

H

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N

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G

A

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P

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Y

Y

G

-

V

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I

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M

-

G

-

P

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F

F

L

A

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N

P

V

L

L

G

M

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K

P

P

C

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A

P

P

G

P

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W

M

G

G

Y

R

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F

A

K

G

A

A

F

D

D

L

V

R

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T

T

G

G

D

K

I

A

A

R

Y

W

M

-

H

-

R

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N

-

G

-

T

-

V

-

R

-

D

-

M

-

T

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P

-

L

-

P

D

L

I

P

P

L

E

K

R

V

-

G

-

V

F

P

P

G

T

I

W

G

S

G

G

P

A

I

L

V

V

T

T

R

D

G

G

V

L

A

A

F

F

L

Y

G

G

A

-

A

T

V

L

D

D

D

G

Y

W

L

F

R

K

A

A

Y

V

D

D

V

R

T

K

T

T

G

G

E

K

Q

V

L

L

W

W

Q

Q

A

Q

R

K

L

L

P

G

A

S

G

G

G

I

Q

I

S

G

T

N

P

P

M

I

T

S

Y

Y

E

E

Q

V

D

G

G

G

R

K

Q

Q

F

Y

V

I

V

S

I

V

V

L

A

S

G

G

G

I

H

G

G

G

S
x
W

V

I

G

G

binding methanol: E173 (≠ D344), W263 (≠ D418), D314 (= D458), W545 (≠ S761)
binding Neodymium Ion: E173 (≠ D344), N259 (= N414), D314 (= D458), D316 (= D460)

6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
26% identity, 80% coverage: 156:776/781 of query aligns to 8:562/563 of 6damA

query
sites
6damA

T

T

A

K

P

N

P

P

G

A

E

N

W

W

H

A

Q

T

Y

W

G

G

R

G

T

N

Q

Y

Y

H

G

G

Q

T

R

R

Y

Y

S

S

P

E

L

L

E

K

Q

Q

I

I

N

N

I

T

Q

S

N

N

V

V

A

K

E

N

L

L

E

Q

Q

P

V

A

W

W

Q

T

Y

F

Q

S

T

T

G

G

D

V

V

L

K

R

L

-

P

-

Q

-

D

-

V

-

T

-

E

-

T

-

T

G

Y

H

Q
x
E

V

G

T

G

P

P

L

L

K

V

V

V

D

N

D

D

R

V

L

M

Y

Y

I

I

C

H

T

T

P

P

H

F

-

P

N

N

L

T

A

V

I

Y

A

A

L

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D

D

A

Q

A

K

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T

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K

A

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W

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D

L

A

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D

T

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I

Q

P

H

V

Q

M

T
x
C

C
|
C

R

D

G

T

V

V

T

N

Y

-

W

-

R
|
R

D

G

P

L

A

A

R

Y

A

G

E

D

G

G

E

-

L

-

C

-

A

-

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K

V

I

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F

L

L

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Q

T

Q

A

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D

D

A

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L

L

I

T

A

A

L

L

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D

A

A

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K

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G

G

A

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-

C

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L

W

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L

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N

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G

M

-

P

-

Y

-

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D

P

P

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K

G

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F

G

Y

M

Y

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N

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|
T

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Q

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A

P

P

V

L

A

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V

V

G

K

G

D

R

K

I

V

I

I

I

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G

G

G

I

A
x
S

V
x
G

N
x
G

D
x
E

N

-

F

-

S

-

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-

F

F

S

G

Q

V

S

R

G

G

V

F

I

L

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A

A

A

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Y

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N

A

I

N

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T

G

G

A

E

L

L

L

D

W

W

N

K

W

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D

Y

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S

G

M

N

G

P

P

D

D

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A

T

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A

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L

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-

P

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K

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W

M

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G

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N

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N

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-

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D

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K

-

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x
T

-

T

W
|
W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

E

E

R

L

G

N

L

L

V

V

Y

Y

I

Y

P

G

L

S

G

G

N
|
N

Q

-

V

-

P

P

D

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L

W

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F

P

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A

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G

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T

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P

H

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D

D

L

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W

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|
D

M

Y

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|
D

V

G

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N

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L

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D

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K

A

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A

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x
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G

G

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Y

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L

L

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G

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|
N

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P

-

L

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P

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L

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K

K

V

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G

Q

V

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P

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x
W

G

S

G

G

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S

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L

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A

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A

G

G

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V

A

V

F

F

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A

-

A

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D

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D

G

Y

Y

L

L

R

K

A

A

Y

V

D

D

V

A

T

K

T

T

G

G

E

K

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E

L

L

W

Y

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A

F

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K

L

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P

A

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G

G

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I

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P

V

M

N

T

T

Y

W

E

E

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Y

D

E

G

G

R

K

Q

Q

F

Y

V

V

V

G

I

V

V

L

A

S

G

G

G

V

H

G

G
|
G

-
x
W

-

S

-

L

S

S

V

S

G

Y

T

T

K

K

P

L

G

G

D

G

Y

T

V

L

I

T

A

V

Y

F

A

A

L

L

P

P

active site: E171 (≠ D344), N259 (= N414), D301 (= D458)
binding pyrroloquinoline quinone: E55 (≠ Q211), C103 (≠ T258), C104 (= C259), R109 (= R266), T153 (= T326), S168 (≠ A341), G169 (≠ V342), G170 (≠ N343), E171 (≠ D344), T239 (vs. gap), W241 (= W396), D303 (= D460), R328 (≠ K485), N394 (= N583), W480 (≠ I696), G543 (= G760), W544 (vs. gap)

A0A3F2YLY8 Lanthanide-dependent methanol dehydrogenase XoxF; Lanthanide-dependent MDH; Ln(3+)-dependent MDH; EC 1.1.2.10 from Methylotuvimicrobium buryatense (Methylomicrobium buryatense) (see paper)
25% identity, 78% coverage: 156:763/781 of query aligns to 34:572/617 of A0A3F2YLY8

query
sites
A0A3F2YLY8

T

T

A

K

P

N

P

P

G

A

E

N

W

W

H

A

Q

T

Y

W

G

G

R

G

T

N

Q

Y

Y

H

G

G

Q

T

R

R

Y

Y

S

S

P

E

L

L

E

K

Q

Q

I

I

N

N

I

T

Q

S

N

N

V

V

A

K

E

N

L

L

E

Q

Q

P

V

A

W

W

Q

T

Y

F

Q

S

T

T

G

G

D

V

V

L

K

R

L

-

P

-

Q

-

D

-

V

-

T

-

E

-

T

-

T

G

Y

H

Q

E

V

G

T

G

P

P

L

L

K

V

V

V

D

N

D

D

R

V

L

M

Y

Y

I

I

C

H

T

T

P

P

H

F

-

P

N

N

L

T

A

V

I

Y

A

A

L

I

D

D

A

Q

A

K

T

T

G

Q

K

A

E

V

A

I

W

W

R

E

F

Y

D

T

A

P

N

Q

S

Q

G

D

L

A

E

D

P

V

D

T

R

I

Q

P

H

V

Q

M

T
x
C

C
|
C

R

D

G

T

V

V

T

N

Y

-

W

-

R
|
R

D

G

P

L

A

A

R

Y

A

G

E

D

G

G

E

-

L

-

C

-

A

-

E

-

R

K

V

I

Y

F

L

L

P

Q

T

Q

A

S

D

D

A

T

R

V

L

L

I

T

A

A

L

L

D

D

A

A

R

K

S

T

G

G

A

-

V

-

C

-

T

-

F

-

A

-

D

-

E

K

G

R

T

V

L

W

H

S

L

V

E

Q

S

N

G

G

M

-

P

-

Y

-

T

D

P

P

E

K

G

L

F

G

Y

M

Y

T

S

N

T
|
T

S

Q

P

A

P

P

V

L

A

V

V

V

G

K

G

D

R

K

I

V

I

I

I

T

G

G

G

I

A
x
S

V
x
G

N
x
G

D
x
E

N

-

F

-

S

-

V

-

F

F

S

G

Q

V

S

R

G

G

V

F

I

L

R

A

A

A

F

Y

D

N

A

I

N

R

T

T

G

G

A

E

L

L

L

D

W

W

N

K

W

G

D

Y

S

S

G

M

N

G

P

P

D

D

E

A

T

D

A

T

P

L

I

I

D

N

-

P

-

T

-

K

-

T

W

W

M

K

N

D

G

G

E

K

T

V

Y

Q

T

P

A

V

N

G

S

K

P

D

N

S

S

S

-

T

-

S

-

T

-

W

-

E

-

G

-

D

-

Q

-

W

-

K

-

I

-

G

-
x
T

-

T

W
|
W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

E

E

R

L

G

N

L

L

V

V

Y

Y

I

Y

P

G

L

S

G

G

N
|
N

Q

-

V

-

P

P

D

S

Q

T

L

W

G

N

F

P

D

A

R

Q

S

R

P

P

A

G

V

D

E

N

E

K

H

W

S

S

S

M

S

S

V

L

V

W

A

A

L

R

D

N

V

A

T

D

T

T

G

G

Q

E

K

V

A

K

W

W

V

V

F

Y

Q

Q

T

M

V

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

Y

D
|
D

V

G

P

I

A

N

Q

E

P

V

V

A

L

L

I

V

D

D

L

Q

D

E

I

I

D

K

G

G

Q

Q

T

M

V

R

P

K

A

T

L

A

V

V

Q

H

P

F

T

D

K
x
R

Q

N

G

G

D

F

I

G

Y

Y

V

T

L

L

N

D

R

R

E

V

T

T

G

G

E

E

P

L

I

L

L

V

P

-

V

-

T

-

E

-

P

-

A

-

P

-

Q

-

E

-

G

-

L

-

P

-

E

-

S

-

P

-

A

-

P

-

T

-

Q

-

P

-

T

-

S

-

A

A

L

E

S

K

F

F

K

D

P

K

A

A

A

V

L

-

R

-

E

-

K

-

D

-

M

N

W

W

G

A

V

S

T

H

I

V

Y

-

D

D

Q

M

L

K

A

S

C

G

R

R

I

P

Q

Q

Y

V

H

V

R

S

L

-

N

Q

Y

Y

E

S

G

T

R

E

Y

Y

T

N

P

G

P

E

S

D

L

V

N

N

G

T

T

E

I

G

V

V

Y
x
C

P

P

G

A

N

A

F

L

G

G

T

S

F

K

N
|
N

W

Q

G

Q

S

P

V

V

A

S

V

Y

D

S

P

P

E

Q

R

T

Q

G

V

Y

M

F

F

Y

G

I

M

S

P

G

T

N

-

H

-

V

-
x
C

-

M

-

D

Y

Y

L

E

P

P

F

F

T

E

V

V

E

E

L

Y

V

T

P

A

A

G

E

Q

D

-

I

-

P

P

P

Y

P

V

G

G

A

A

N

T

E

L

T

S

A

M

S

F

E

P

Q

A

G

G

L

K

N

D

R

-

N

-

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G

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A

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-

Y

-

G

-

V

V

I

I

M

T

G

G

P

K

F

E

L

D

G

G

P

S

L

N

G

N

V

L

P

-

C

-

S

-

A

-

P

-

W

-

G

G

Y

Q

V

F

A

T

G

A

A

W

D

D

L

A

R

T

T

T

G

G

D

K

I

I

A

I

Y

W

M

S

H

N

R

-

N

-

G

-

T

-

V

-

R

-

D

-

M

-

T

-

P

-

L

-

P

-

L

-

P

-

L

-

K

K

V

E

G

Q

V

F

P

S

G

V

I
x
W

G

S

G

G

P

S

I

L

V

A

T

T

R

A

G

G

V

V

A

V

F

F

L

Y

G

G

A

-

A

T

V

L

D

E

D

G

Y

Y

L

L

R

K

A

A

Y

V

D

D

V

A

T

K

T

T

G

G

E

K

Q

E

L

L

W

Y

Q

R

A

F

R

K

L

T

P

P

A

S

G

G

G

I

Q

I

S

G

T

N

P

V

M

N

T

T

Y

W

E

E

Q

Y

D

E

G

G

R

K

Q

Q

F

Y

V

V

V

G

I

V

V

L

A

S

G

G

G

V

H

G

G

G

S
x
W

V

A

G

G

C129 (≠ T258) modified: Disulfide link with 130
C130 (= C259) modified: Disulfide link with 129
R135 (= R266) binding pyrroloquinoline quinone
T179 (= T326) binding pyrroloquinoline quinone
S194 (≠ A341) binding pyrroloquinoline quinone
G195 (≠ V342) binding pyrroloquinoline quinone
G196 (≠ N343) binding pyrroloquinoline quinone
E197 (≠ D344) binding La(3+)
T265 (vs. gap) binding pyrroloquinoline quinone
W267 (= W396) binding pyrroloquinoline quinone
N285 (= N414) binding La(3+)
D327 (= D458) binding La(3+)
D329 (= D460) binding La(3+)
R354 (≠ K485) binding pyrroloquinoline quinone
C412 (≠ Y575) modified: Disulfide link with 441
N420 (= N583) binding pyrroloquinoline quinone
C441 (vs. gap) modified: Disulfide link with 412
W506 (≠ I696) binding pyrroloquinoline quinone
W570 (≠ S761) binding pyrroloquinoline quinone

1lrwA Crystal structure of methanol dehydrogenase from p. Denitrificans (see paper)
23% identity, 77% coverage: 159:757/781 of query aligns to 11:536/600 of 1lrwA

query
sites
1lrwA

P

P

G

A

E

N

W

W

H

V

Q

M

Y

T

G

G

R

R

T

D

Q

Y

Y

N

G

A

Q

Q

R

N

Y

Y

S

S

P

E

L

M

E

T

Q

D

I

I

N

N

I

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N

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L

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W

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G

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L

-

P

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-

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-

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-

T

-

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G

Y

H

Q
x
E

V

G

T

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P

P

L

L

K

V

V

V

D

G

D

D

R

R

L

M

Y

F

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I

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H

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P

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N

N

L

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A

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A

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L

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D

D

A

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-

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|
C

-
x
C

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|
R

G

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Y

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L

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-

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-

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|
T

S

I

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A

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P

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Y

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V

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K

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R

L

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A
x
S

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N
x
A

D
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-

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-

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-

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W

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D

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-

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P

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H

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Y

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|
W

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P

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D

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|
D

M

Y

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G

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L

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-

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R

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G

Q

A

F

P

F

Y

V

G

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A

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L

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M

F

Y

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L

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A

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L

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L

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-

L

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K

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x
W

G

G

P

T

I

M

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A

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T

R

A

G

G

V

L

A

T

F

F

L

Y

G

-

A

A

A

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V

L

D

D

D

G

Y

F

L

I

R

K

A

A

Y

R

D

D

V

S

T

D

T

T

G

G

E

D

Q

L

L

L

W

W

Q

K

A

F

R

K

L

L

P

P

A

S

G

G

G

V

Q

I

S

G

T

H

P

P

M

M

T

T

Y

Y

E

K

Q

H

D

D

G

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R

R

Q

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F

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V

V

A

I

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V

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A

Y

G

G

active site: E177 (≠ D344), N261 (= N414), D303 (= D458)
binding calcium ion: E177 (≠ D344), N261 (= N414), D303 (= D458)
binding pyrroloquinoline quinone: E55 (≠ Q211), C103 (= C259), C104 (vs. gap), R109 (= R260), T159 (= T326), S174 (≠ A341), G175 (≠ V342), A176 (≠ N343), E177 (≠ D344), T241 (≠ N394), W243 (= W396), R331 (≠ K485), W476 (≠ I696)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 540

6oc6A Lanthanide-dependent methanol dehydrogenase xoxf from methylobacterium extorquens, in complex with lanthanum and pyrroloquinoline quinone (see paper)
24% identity, 76% coverage: 173:763/781 of query aligns to 25:539/579 of 6oc6A

query
sites
6oc6A

R

R

Y

Y

S

S

P

K

L

L

E

D

Q

Q

I

I

N

N

I

A

Q

S

N

N

V

V

A

K

E

N

L

L

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V

V

A

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W

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G

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L

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Y

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x
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N

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A

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-

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Q

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C
|
C

R
x
C

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D

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-

T

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Y

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N

R
|
R

D

G

P

L

A

A

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A

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A

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C

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D

A

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G

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N

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G

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G

-

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Y

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|
T

S

A

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A

P

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G

K

G

D

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K

I

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I

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G

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A
x
S

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x
G

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x
G

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x
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-

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-

F

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D

A

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N

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A

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W

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Y

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G

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C

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W

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W

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E

K

R

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L

L

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Y

I

Y

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G

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G

G

N
|
N

Q

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V

-

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W

G

N

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H

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D

D

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|
D

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D

V

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A

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R

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A

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x
D

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A

A

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E

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R

K

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A

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N

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A

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Y

G

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-

I

-

M

V

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A

F

T

L

L

G

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P

M

L

Y

G

P

V

A

P

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C

G

S

S

A

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P

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M

G

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Y

N

V

F

A

-

G

-

A

-

D

-

L

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R

-

T

-

G

-

D

-

I

I

A

A

Y

W

M

D

H

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R

L

N

Q

G

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V

I

R

K

D

W

M

S

T

N

P

P

L

E

P

Q

L

F

P

S

L

A

K
x
W

V

G

G

G

V

A

P

L

G

A

I

T

G

A

G

G

P

D

I

V

V

V

T

F

R

Y

G

G

G

-

V

-

A

-

F

-

L

-

G

-

A

-

A

T

V

L

D

E

D

G

Y

F

L

L

R

K

A

A

Y

V

D

D

V

S

T

K

T

T

G

G

E

K

Q

E

L

L

W

Y

Q

K

A

F

R

K

L

T

P

P

A

S

G

G

G

I

Q

I

S

G

T

N

P

V

M

M

T

T

Y

Y

E

E

Q

H

D

K

G

G

R

K

Q

Q

F

H

V

V

V

A

I

V

V

L

A

S

G

G

G

V

H

G

G
|
G

S
x
W

V

A

G

G

active site: E171 (≠ D344), N255 (= N414), D297 (= D458)
binding pyrroloquinoline quinone: E55 (≠ Q211), C103 (= C259), C104 (≠ R260), R109 (= R266), T153 (= T326), S168 (≠ A341), G169 (≠ V342), G170 (≠ N343), E171 (≠ D344), W237 (= W396), D299 (= D460), R324 (≠ K485), D395 (≠ N583), W473 (≠ K690), G536 (= G760), W537 (≠ S761)

C5B120 Lanthanide-dependent methanol dehydrogenase; Lanthanide-dependent MDH; Ln(3+)-dependent MDH; La(3+)- and PQQ-dependent MDH; La(3+)-dependent methanol dehydrogenase; La(3+)-dependent MDH; EC 1.1.2.10 from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) (see 2 papers)
24% identity, 76% coverage: 173:763/781 of query aligns to 46:560/601 of C5B120

query
sites
C5B120

R

R

Y

Y

S

S

P

K

L

L

E

D

Q

Q

I

I

N

N

I

A

Q

S

N

N

V

V

A

K

E

N

L

L

E

Q

Q

V

V

A

W

W

Q

T

Y

F

Q

S

T

T

G

G

D

V

V

L

K

R

L

-

P

-

Q

-

D

-

V

-

T

-

E

-

T

-

T

G

Y

H

Q

E

V

G

T

S

P

P

L

L

K

V

V

V

D

G

D

N

R

I

L

M

Y

Y

I

V

C

H

T

T

P

P

H

F

-

P

N

N

L

I

A

V

I

Y

A

A

L

L

D

D

A

L

A

D

T

Q

G

G

-

A

K

K

E

I

A

V

W

W

R

K

F

Y

D

E

A

P

N

K

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Q

G

-

L

-

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D

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P

D

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I

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M

C

C

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C

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-

T

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Y

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|
R

D

G

P

L

A

A

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Y

A

A

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D

G

G

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A

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C

-

A

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-

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-

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I

Y

L

L

L

P

H

T

Q

A

A

D

D

A

T

R

T

L

L

I

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A

S

L

L

D

D

A

A

R

K

S

S

G

G

A

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V

V

C

-

T

-

F

-

A

-

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-

G

-

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N

L

W

H

S

L

V

E

K

S

N

G

G

M

D

P

P

Y

S

T

K

P

G

E

E

G

-

F

-

Y

-

Y

T

S

N

T
|
T

S

A

P

T

P

V

V

L

A

P

V

V

G

K

G

D

R

K

I

V

I

I

I

V

G

G

G

I

A
x
S

V
x
G

N
x
G

D
x
E

N

-

F

-

S

-

V

-

F

F

S

G

Q

V

S

Q

G

C

V

H

I

V

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T

A

A

F

Y

D

D

A

L

N

K

T

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G

G

A

K

L

K

L

V

W

W

N

R

W

G

D

Y

S

S

G

I

N

G

P

P

D

D

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D

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Q

A

L

P

I

I

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D

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P

-

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-

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-

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-

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-

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-

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W

W

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N

E

G

G

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Q

Y

W

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K

A

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N

G

S

G

P

G

N

C

S

T

W
|
W

S

G

V

W

F

F

S

S

V

Y

D

D

E

P

E

K

R

L

G

D

L

L

V

M

Y

Y

I

Y

P

G

L

S

G

G

N
|
N

Q

-

V

-

P

P

D

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Q

T

L

W

G

N

F

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D

K

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N

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K

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M

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V

I

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W

A

A

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N

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P

T

D

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G

G

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M

K

A

A

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W

V

V

F

Y

Q

Q

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M

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T

H

P

H

H

D

D

L

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W

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|
D

M

F

D
|
D

V

G

P

I

A

N

Q

E

P

M

V

I

L

L

I

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D

D

L

Q

D

K

I

F

D

D

G

G

Q

K

T

D

V

R

P

P

A

L

L

L

V

T

Q

H

P

F

T

D

K
x
R

Q

N

G

G

D

F

I

G

Y

Y

V

T

L

L

N

D

R

R

E

A

T

T

G

G

E

E

P

V

I

L

L

V

P

A

V

E

T

K

E

F

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D

P

P

A

V

P

-

Q

-

E

-

G

-

L

V

P

N

E

W

E

A

S

T

P

K

A

V

P

D

T

L

Q

D

P

K

T

G

S

S

A

K

L

T

S

Y

F

G

K

R

P

P

A

L

A

V

L

V

R

S

E

K

K

-

D

-

M

-

W

-

G

-

V

-

T

-

I

-

Y

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D

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Q

-

L

-

A

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C

-

R

-

I

-

Q

-

Y

-

H

-

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-

L

-

N

-

Y

Y

E

S

G

T

R

E

Y

Q

T

N

P

G

P

E

S

D

L

V

N

N

G

S

T

K

I

G

V

I

Y

C

P

P

G

A

N

A

F

L

G

G

T

T

F

K

N

D

W

Q

G

Q

S

P

V

A

A

A

V

F

D

S

P

P

E

-

R

K

Q

T

V

G

M

L

F

F

G

Y

M

V

P

P

T

T

-

N

-

H

-

V

-

C

-

M

-

D

Y

Y

L

E

P

P

F

F

T

R

V

V

E

T

L

Y

V

T

P

P

A

-

E

-

D

-

I

-

P

-

G

-

A

-

N

-

E

-

T

-

A

-

S

-

E

-

Q

-

G

-

L

-

N

-

R

-

N

-

E

-

G

G

A

Q

P

P

Y

Y

G

-

V

-

I

-

M

V

G

G

P

A

F

T

L

L

G

S

P

M

L

Y

G

P

V

A

P

P

C

G

S

S

A

H

P

G

W

M

G

G

Y

N

V

F

A

-

G

-

A

-

D

-

L

-

R

-

T

-

G

-

D

-

I

I

A

A

Y

W

M

D

H

N

R

L

N

Q

G

G

T

K

V

I

R

K

D

W

M

S

T

N

P

P

L

E

P

Q

L

F

P

S

L

A

K
x
W

V

G

G

G

V

A

P

L

G

A

I

T

G

A

G

G

P

D

I

V

V

V

T

F

R

Y

G

G

G

-

V

-

A

-

F

-

L

-

G

-

A

-

A

T

V

L

D

E

D

G

Y

F

L

L

R

K

A

A

Y

V

D

D

V

S

T

K

T

T

G

G

E

K

Q

E

L

L

W

Y

Q

K

A

F

R

K

L

T

P

P

A

S

G

G

G

I

Q

I

S

G

T

N

P

V

M

M

T

T

Y

Y

E

E

Q

H

D

K

G

G

R

K

Q

Q

F

H

V

V

V

A

I

V

V

L

A

S

G

G

G

V

H

G

G

G

S
x
W

V

A

G

G

R130 (= R266) binding pyrroloquinoline quinone
T174 (= T326) binding pyrroloquinoline quinone
S189 (≠ A341) binding pyrroloquinoline quinone
G190 (≠ V342) binding pyrroloquinoline quinone
G191 (≠ N343) binding pyrroloquinoline quinone
E192 (≠ D344) binding La(3+)
W258 (= W396) binding pyrroloquinoline quinone
N276 (= N414) binding La(3+)
D318 (= D458) binding La(3+)
D320 (= D460) binding La(3+); mutation to A: Loss of methanol dehydrogenase activity. In contrast to wild-type, the mutant cells are incapable of growth with methanol and La(3+). The mutant protein is unable to bind La(3+) and is loaded with Ca(2+) regardless of whether or not La(3+) is included in the growth medium, but is inactive.
R345 (≠ K485) binding pyrroloquinoline quinone
W494 (≠ K690) binding pyrroloquinoline quinone
W558 (≠ S761) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:21 signal peptide

4aahA Methanol dehydrogenase from methylophilus w3a1 (see paper)
23% identity, 75% coverage: 170:752/781 of query aligns to 22:522/571 of 4aahA

query
sites
4aahA

Y

Y

G

S

Q

Q

R

H

Y

N

S

S

P

P

L

L

E

A

Q

Q

I

I

N

N

I

K

Q

S

N

N

V

V

A

K

E

N

L

V

E

K

Q

A

V

A

W

W

Q

S

Y

F

Q

S

T

T

G

G

D

V

V

L

K

-

L

-

P

-

Q

-

D

-

V

-

T

-

E

-

T

N

T

G

Y

H

Q
x
E

V

G

T

A

P

P

L

L

K

V

V

I

D

G

D

D

R

M

L

M

Y

Y

I

V

C

H

T

S

-

A

-

F

P

P

H

N

N

N

L

T

A

Y

I

A

A

L

L

N

D

L

A

N

A

D

T

P

G

G

K

K

E

I

A

V

W

W

R

Q

F

H

D

K

A

P

N

K

S

Q

G

-

L

-

E

D

P

A

D

S

R

T

Q

K

H

A

Q

V

T

M

C
|
C

R
x
C

G

D

V

V

T

V

Y

D

W

-

R
|
R

D

G

P

L

A

A

R

Y

A

G

E

A

G

G

E

Q

L

I

C

V

A

K

E

K

R

Q

V

-

Y

-

L

-

P

-

T

-

A

A

D

N

A

G

R

H

L

L

I

L

A

A

L

L

D

D

A

A

R

K

S

T

G

G

A

K

V

I

C

-

T

-

F

-

A

-

D

-

E

-

G

-

T

N

L

W

H

E

L

V

E

E

S

V

G

C

M

D

P

P

Y

K

T

V

P

G

E

S

G

T

F

L

Y

-

S

-

T

T

S

Q

P

A

P

P

V

F

A

V

V

A

G

K

G

D

R

T

I

V

I

L

I

M

G

G

G

C

A
x
S

V

G

N
x
A

D
x
E

N

-

F

-

S

-

V

-

F

L

S

G

Q

V

S

R

G

G

V

A

I

V

R

N

A

A

F

F

D

D

A

L

N

K

T

T

G

G

A

E

L

L

L

K

W

W

-

R

-

A

-

F

-

A

-

T

-

G

-

S

-

D

-

S

-

V

-

R

-

L

-

A

-

K

N

D

W

F

D

N

S

S

G

A

N

N

P

P

D

H

E

Y

-

G

-

Q

-

F

-

G

T

L

A

G

P

T

I

K

D

T

W

W

M

-

N

E

G

G

E

D

T

A

Y

W

T

K

A

I

N

G

S

G

P

G

N

T

S

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

E

K

R

L

G

N

L

L

V

F

Y

Y

I

Y

P

G

L

S

G

G

N
|
N

Q

P

V

A

P

P

D

-

Q

-

L

W

G

N

F

E

D

T

R

M

S

R

P

P

A

G

V

D

E

N

E

K

H

W

S

T

S

M

S

T

V

I

V

W

A

G

L

R

D

D

V

L

T

D

T

T

G

G

Q

M

K

A

A

K

W

W

V

G

F

Y

Q

Q

T

K

V

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

F

D

A

V

G

P

V

A

N

Q

Q

P

M

V

V

L

L

I

T

D

D

L

Q

D

P

I

V

D

N

G

G

Q

K

T

M

V

T

P

P

A

L

L

L

V

S

Q

H

P

I

T

D

K
x
R

Q

N

G

G

D

I

I

L

Y

Y

V

T

L

L

N

N

R

R

E

E

T

N

G

G

E

N

P

L

I

I

L

V

P

A

V

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T

K

E

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E

D

P

P

A

A

P

V

Q

N

E

-

G

-

L

-

P

-

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-

S

-

P

-

A

-

P

-

T

-

Q

-

P

-

T

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S

-

A

-

L

-

S

V

F

F

K

K

P

K

A

V

A

D

L

L

R

K

E

T

K

-

D

-

M

-

W

-

G

G

V

T

T

P

I

V

Y

R

D

D

-

P

Q

E

L

F

A

A

C

T

R

R

I

M

Q

D

Y

H

H

-

R

-

L

-

N

-

Y

-

E

-

G

-

R

-

Y

-

T

-

P

-

S

-

L

-

N

K

G

G

T

T

I

N

V

I

Y

C

P

P

G

S

N

A

F

M

G

G

T

F

F

H

N
|
N

W

Q

G

G

S

V

V

D

A

S

V

Y

D

D

P

P

E

E

R

S

Q

R

V

T

M

L

F

Y

G

A

M

G

P

L

T

N

Y

H

L

I

P

C

F

M

T

D

V

W

E

E

-

P

-

F

L

M

V

L

P

P

A

Y

E

-

D

-

I

-

P

-

G

-

A

-

N

-

E

-

T

-

A

-

S

-

E

-

Q

-

G

-

L

-

N

-

R

R

N

A

E

G

G

Q

A

F

P

F

Y

V

G

G

V

A

I

T

M

L

G

A

P

M

F

Y

L

P

G

G

P

P

L

N

G

G

V

P

P

-

C

-

S

T

A

K

P

K

P

E

W

M

G

G

Y

Q

V

I

A

R

G

A

A

F

D

D

L

L

R

T

T

T

G

G

D

K

I

A

A

K

Y

W

M

T

H

K

R

W

N

E

G

-

T

-

V

-

R

-

D

-

M

-

T

-

P

-

L

-

P

-

L

-

P

-

L

-

K

-

V

-

G

K

V

F

P

A

G

A

I
x
W

G

G

P

T

I

L

V

Y

T

T

R

K

G

G

V

L

A

V

F

W

L

Y

G

-

A

A

A

T

V

L

D

D

D

G

Y

Y

L

L

R

K

A

A

Y

L

D

D

V

N

T

K

T

D

G

G

E

K

Q

E

L

L

W

W

Q

N

A

F

R

K

L

M

P

P

A

S

G

G

Q

I

S

G

T

S

P

P

M

M

T

T

Y

Y

E

S

Q

F

D

K

G

G

R

K

Q

Q

F

Y

V

I

active site: E171 (≠ D344), N255 (= N414), D297 (= D458)
binding calcium ion: E171 (≠ D344), N255 (= N414)
binding pyrroloquinoline quinone: E55 (≠ Q211), C103 (= C259), C104 (≠ R260), R109 (= R266), S168 (≠ A341), A170 (≠ N343), E171 (≠ D344), W237 (= W396), R324 (≠ K485), N387 (= N583), W467 (≠ I696)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 531

Q88JH0 Quinoprotein alcohol dehydrogenase PedH; Lanthanide-dependent pyrroloquinoline quinone-dependent alcohol dehydrogenase; Lanthanide-dependent PQQ-ADH; EC 1.1.2.- from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
28% identity, 42% coverage: 171:500/781 of query aligns to 53:365/595 of Q88JH0

query
sites
Q88JH0

G

G

Q

Q

R

R

Y

Y

S

S

P

P

L

L

E

D

Q

L

I

L

N

N

I

V

Q

N

N

N

V

V

A

K

E

E

L

L

E

R

Q

P

V

V

W

W

Q

A

Y

F

Q

S

T

F

G

G

D

G

V

E

K

K

L

-

P

-

Q

-

D

-

V

-

T

-

E

Q

T

R

T

G

Y

Q

Q
|
Q

V

A

T

Q

P

P

L

L

K

I

V

K

D

D

D

G

R

V

L

M

Y

Y

I

L

C

T

T

G

P

S

H

Y

N

S

L

R

A

V

I

F

A

A

L

V

D

D

A

A

A

R

T

T

G

G

K

K

E

K

A

L

W

W

R

Q

F

Y

D

D

A

A

N

R

S

L

G

-

L

-

E

-

P

P

D

D

R

D

Q

I

H

R

Q

P

T
x
C

C
|
C

-

D

-

V

-

I

-

N

R
|
R

G

G

V

V

T

A

Y

-

W

-

R

-

D

-

P

-

A

-

R

-

A

-

E

-

G

-

E

-

L

L

C

Y

A

G

E

N

R

L

V

V

Y

F

L

F

P

G

T

T

A

L

D

D

A

A

R

K

L

L

I

V

A

A

L

L

D

N

A

K

R

D

S

T

G

G

A

K

V

V

C

V

T

-

F

-

F

W

A

S

D

K

E

K

G

V

T

A

L

D

H

H

L

K

E

E

S

G

G

-

M

-

P

-

Y

-

T

-

P

-

E

-

G

-

F

-

Y

Y

Y

S

S

I

T
x
S

S

A

P

A

P

P

V

M

A

I

V

V

G

N

G

G

R

K

I

L

I

I

I

T

G

G

G

V

A

A

V
x
G

N
x
G

D
x
E

N

-

F

-

S

-

V

-

F

F

S

G

Q

V

S

V

G

G

V

K

I

I

R

Q

A

A

F

Y

D

N

A

P

N

E

T

N

G

G

A

E

L

L

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W

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M

-

R

-

P

-

T

-

V

-

E

-

G

-

H

-

M

-

G

-

Y

-

V

W

Y

D

K

S

D

G

G

N

K

P

A

D

I

E

E

T

N

A

G

P

I

I

S

D

G

W

G

M

E

N

A

G

G

E

K

T

T

Y

W

T

P

A

G

N

D

S

L

P

W

N

K

S

T

-

G

-

A

-

P

W
|
W

S

L

V

G

F

G

S

Y

V

Y

D

D

E

P

E

E

R

T

G

N

L

L

V

I

Y

L

I

F

P

G

L

T

G

G

N
|
N

Q

P

V

A

P

P

D

-

Q

-

L

W

G

N

F

S

D

H

R

L

S

R

P

P

A

G

V

D

E

N

E

L

H

Y

S

S

V

R

V

L

A

A

L

L

D

N

V

P

T

D

G

G

Q

T

K

I

A

K

W

W

V

H

F

F

Q

Q

T

S

V

T

H

P

H

H

D

D

L

G

W

W

D
|
D

M

F

D
|
D

V

G

P

V

A

N

Q

E

P

L

V

I

L

S

I

F

D

N

L

Y

D

K

I

D

D

G

G

G

Q

K

T

E

V

V

P

K

A

A

L

A

V

A

Q

T

P

A

T

D

K
x
R

Q

N

G

G

D

F

I

F

Y

Y

V

V

L

L

N

D

R

R

E

T

T

N

G

G

E

K

P

F

I

I

Q87 (= Q211) binding pyrroloquinoline quinone
C131 (≠ T258) modified: Disulfide link with 132
C132 (= C259) modified: Disulfide link with 131
R137 (= R260) binding pyrroloquinoline quinone
S181 (≠ T326) binding pyrroloquinoline quinone
G197 (≠ V342) binding pyrroloquinoline quinone
G198 (≠ N343) binding pyrroloquinoline quinone
E199 (≠ D344) binding Pr(3+)
W263 (= W396) binding pyrroloquinoline quinone
N281 (= N414) binding Pr(3+)
D323 (= D458) binding Pr(3+)
D325 (= D460) binding Pr(3+)
R350 (≠ K485) binding pyrroloquinoline quinone

Sites not aligning to the query:

412 F→I: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ser-561 or Gln-561.; F→V: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Ala-561.
417 binding pyrroloquinoline quinone
493 binding pyrroloquinoline quinone
557 binding pyrroloquinoline quinone
561 W→A: High decrease in affinity for ethanol, and in contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA) and can also oxidize 5-(hydroxymethyl)furfural (HMF) and 5-formylfurfural (FFF); when associated with Val-412.; mutation W->S,Q: In contrast to wild-type, this mutant is able to oxidize 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA); when associated with Ile-412.

6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
28% identity, 42% coverage: 171:500/781 of query aligns to 26:338/562 of 6zcvA

query
sites
6zcvA

G

G

Q

Q

R

R

Y

Y

S

S

P

P

L

L

E

D

Q

L

I

L

N

N

I

V

Q

N

N

N

V

V

A

K

E

E

L

L

E

R

Q

P

V

V

W

W

Q

A

Y

F

Q

S

T

F

G

G

D

G

V

E

K

K

L

-

P

-

Q

-

D

-

V

-

T

-

E

Q

T

R

T

G

Y

Q

Q
|
Q

V

A

T

Q

P

P

L

L

K

I

V

K

D

D

D

G

R

V

L

M

Y

Y

I

L

C

T

T

G

P

S

H

Y

N

S

L

R

A

V

I

F

A

A

L

V

D

D

A

A

A

R

T

T

G

G

K

K

E

K

A

L

W

W

R

Q

F

Y

D

D

A

A

N

R

S

L

G

-

L

-

E

-

P

P

D

D

R

D

Q

I

H

R

Q

P

T
x
C

C
|
C

-

D

-

V

-
x
I

-

N

R
|
R

G

G

V

V

T

A

Y

-

W

-

R

-

D

-

P

-

A

-

R

-

A

-

E

-

G

-

E

-

L

L

C

Y

A

G

E

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R

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F

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F

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G

T

T

A

L

D

D

A

A

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A

A

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N

A

K

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G

A

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-

F

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W

A

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K

E

K

G

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G

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M

-

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Y

-

T

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P

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F

-

Y

Y

Y

S

S

I

T
x
S

S

A

P

A

P

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V

M

A

I

V

V

G
x
N

G

G

R
x
K

I

L

I

I

I

T

G

G

G

V

A

A

V
x
G

N
x
G

D
x
E

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-

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-

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-

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-

F

F

S

G

Q

V

S

V

G

G

V

K

I

I

R

Q

A

A

F

Y

D

N

A

P

N

E

T

N

G

G

A

E

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L

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M

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R

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P

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T

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V

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-

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W

Y

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S

D

G

G

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I

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N

A

G

P

I

I

S

D

G

W

G

M

E

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A

G

G

E

K

T

T

Y

W

T

P

A

G

N

D

S

L

P

W

N

K

S

T

-

G

-

A

-

P

W
|
W

S

L

V

G

F

G

S

Y

V

Y

D

D

E

P

E

E

R

T

G

N

L

L

V

I

Y

L

I

F

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G

L

T

G

G

N
|
N

Q

P

V

A

P

P

D

-

Q

-

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W

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F

S

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H

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L

S

R

P

P

A

G

V

D

E

N

E

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H

Y

S

S

V

R

V

L

A

A

L

L

D

N

V
x
P

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x
D

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D

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G

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I

A

K

W

W

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H

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F

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H

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D

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L

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G

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E

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L

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I

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I

F

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N

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Y

D

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I

D

D

G

G

G

Q

K

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V

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P

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A

A

L

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x
R

Q

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G

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R

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T

N

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I

active site: E172 (≠ D344), N254 (= N414), D296 (= D458)
binding calcium ion: N161 (≠ G333), K163 (≠ R335), P278 (≠ V440), D279 (≠ T441)
binding pyrroloquinoline quinone: Q60 (= Q211), C104 (≠ T258), C105 (= C259), I108 (vs. gap), R110 (= R260), S154 (≠ T326), G170 (≠ V342), G171 (≠ N343), E172 (≠ D344), W236 (= W396), D298 (= D460), R323 (≠ K485)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 390, 466, 529, 530

1kb0A Crystal structure of quinohemoprotein alcohol dehydrogenase from comamonas testosteroni (see paper)
27% identity, 44% coverage: 161:500/781 of query aligns to 28:350/670 of 1kb0A

query
sites
1kb0A

E

D

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W

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Y

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L

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D

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Q

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I

N

N

I

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Q

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L

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W

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L

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x
E

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A

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T

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K

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V

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D

D

D

G

R

I

L

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Y

Y

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S

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V

A

V

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H

A

A

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I

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D

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A

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T

G

G

K

N

E

R

A

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-

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C
|
C

-
x
C

-

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|
R

G

G

V

V

T

A

Y

L

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W

R

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D

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P

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A

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R

-

A

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E

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G

-

E

-

L

-

C

-

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Y

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A

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A

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R

L

L

I

I

A

A

L

L

D

D

A

A

R

A

S

T

G

G

A

K

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E

C

V

-

W

-

H

-

Q

-

N

T

T

F

F

F

E

A

G

D

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E

K

G

G

T

S

L

L

H

T

L

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E

-

S

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M

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-

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-

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-

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|
T

S

G

P

A

P

P

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R

A

V

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F

G

K

G

G

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K

I

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I

I

G

G

G

-

A

-

V

-

N

-

D

N

N
x
G

F
x
G

S
x
A

V
x
E

F

Y

S

G

Q

V

S

R

G

G

V

Y

I

I

R

T

A

A

F

Y

D

D

A

A

N

E

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G

G

A

E

L

R

L

K

W

W

N

R

W

W

D

F

S

S

G

V

N

P

P

G

D

D

E

P

T

S

A

K

P

P

I

F

D

E

-

D

-

E

-

S

-

M

-

K

-

R

-

A

-

R

-

T

W

W

M

D

-

P

N

S

G

G

E

K

T

W

Y

W

T

E

A

A

N

G

S

G

P

G

N

G

S
x
T

-

M

W
|
W

S

D

V

S

F

M

S

T

V

F

D

D

E

A

E

E

R

L

G

N

L

T

V

M

Y

Y

I

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G

L

T

G

G

N
|
N

Q

G

V

S

P

P

D

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Q

S

L

H

G

K

F

V

D

-

R

R

S

S

P

P

A

K

V

G

E

G

E

D

-

N

-

L

H

Y

S

L

S

A

S

S

V

I

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V

A

A

L

L

D

D

V

P

T

D

T

T

G

G

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K

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A

K

W

W

V

H

F

Y

Q

Q

T

E

V

T

H

P

H

G

D

D

L

N

W

W

D
|
D

M

Y

D

T

V

S

P

T

A

Q

Q

P

P

M

V

I

L

L

I

A

D

D

L

I

D

K

I

I

D

A

G

G

Q

K

T

P

V

R

P

K

A

V

L

I

V

L

Q

H

P

A

T

P

K
|
K

Q

N

G

G

D

F

I

F

Y

F

V

V

L

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N

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I

I

active site: E185 (≠ V348), N263 (= N414), D308 (= D458)
binding calcium ion: E185 (≠ V348), N263 (= N414), D308 (= D458)
binding pyrroloquinoline quinone: E70 (≠ Q211), C116 (= C259), C117 (vs. gap), R122 (= R260), T167 (= T326), G182 (≠ N345), G183 (≠ F346), A184 (≠ S347), E185 (≠ V348), T243 (≠ S395), W245 (= W396), D308 (= D458), K335 (= K485)
binding tetrahydrofuran-2-carboxylic acid: C116 (= C259), C117 (vs. gap), E185 (≠ V348), D308 (= D458)

Sites not aligning to the query:

binding heme c: 598, 599, 602, 603, 617, 620, 631, 637, 640, 642, 643, 645
binding pyrroloquinoline quinone: 394, 395, 479, 543, 544
binding tetrahydrofuran-2-carboxylic acid: 389

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
27% identity, 44% coverage: 161:500/781 of query aligns to 59:381/708 of Q46444

query
sites
Q46444

E

D

W

W

H

P

Q

T

Y

I

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Q

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N

I

A

Q

A

N

N

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V

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L

-

P

-

Q

-

D

-

V

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V

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x
E

V

A

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T

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P

L

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D

D

G

R

I

L

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Y

I

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A

V

I

H

A

A

L

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D

D

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A

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T

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G

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E

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A

I

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W

R

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Y

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-

G

-

L

I

E

D

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R

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G

H

F

Q

K

T

G

C
|
C

-
x
C

-

D

-

V

-

N

R
|
R

G

G

V

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A

Y

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W

R

K

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P

-

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-

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C

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A

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A

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L

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D

A

A

R

A

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G

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W

-

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-

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-

N

T

T

F

F

F

E

A

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G

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T

S

L

L

H

T

L

I

E

-

S

-

G

-

M

-

P

-

Y

-

T

-

P

-

E

-

G

-

F

-

Y

-

S

-

T
|
T

S

G

P

A

P

P

V

R

A

V

V

F

G

K

G

G

R

K

I

V

I

I

G

G

G

-

A

-

V

-

N

-

D

K

N

R

F
x
G

S
x
A

V
x
E

F

Y

S

G

Q

V

S

R

G

G

V

Y

I

I

R

T

A

A

F

Y

D

D

A

A

N

E

T

T

G

G

A

E

L

R

L

K

W

W

N

R

W

W

D

F

S

S

G

V

N

P

P

G

D

D

E

P

T

S

A

K

P

P

I

F

D

E

-

D

-

E

-

S

-

M

-

K

-

R

-

A

-

R

-

T

W

W

M

D

-

P

N

S

G

G

E

K

T

W

Y

W

T

E

A

A

N

G

S

G

P

G

N

G

S
x
T

-

M

W

W

S

D

V

S

F

M

S

T

V

F

D

D

E

A

E

E

R

L

G

N

L

T

V

M

Y

Y

I

V

P

G

L

T

G

G

N
|
N

Q

G

V

S

P

P

D

W

Q

S

L

H

G

K

F

V

D

-

R

R

S

S

P

P

A

K

V

G

E

G

E

D

-

N

-

L

H

Y

S

L

S

A

S

S

V

I

V

V

A

A

L

L

D

D

V

P

T

D

T

T

G

G

Q

K

K

Y

A

K

W

W

V

H

F

Y

Q

Q

T

E

V

T

H

P

H

G

D

D

L

N

W

W

D
|
D

M

Y

D

T

V

S

P

T

A

Q

Q

P

P

M

V

I

L

L

I

A

D

D

L

I

D

K

I

I

D

A

G

G

Q

K

T

P

V

R

P

K

A

V

L

I

V

L

Q

H

P

A

T

P

K
|
K

Q

N

G

G

D

F

I

F

Y

F

V

V

L

L

N

D

R

R

E

T

T

N

G

G

E

K

P

F

I

I

E101 (≠ Q211) binding pyrroloquinoline quinone
C147 (= C259) modified: Disulfide link with 148
C148 (vs. gap) modified: Disulfide link with 147
R153 (= R260) binding pyrroloquinoline quinone
T198 (= T326) binding pyrroloquinoline quinone
GA 214:215 (≠ FS 346:347) binding pyrroloquinoline quinone
E216 (≠ V348) binding Ca(2+)
T274 (≠ S395) binding pyrroloquinoline quinone
N294 (= N414) binding Ca(2+)
D339 (= D458) binding Ca(2+)
K366 (= K485) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:31 signal peptide
425:426 binding pyrroloquinoline quinone
575 binding pyrroloquinoline quinone
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

Q9Z4J7 Quinoprotein ethanol dehydrogenase; QEDH; Quinoprotein alcohol dehydrogenase (cytochrome c); Quinoprotein alcohol dehydrogenase (cytochrome c550); EC 1.1.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
26% identity, 43% coverage: 164:498/781 of query aligns to 54:391/623 of Q9Z4J7

query
sites
Q9Z4J7

Q

Q

Y

Y

G

G

R

M

T

G

Q

T

Y

H

G

A

Q

Q

R

R

Y

W

S

S

P

P

L

L

E

K

Q

Q

I

V

N

N

I

A

Q

D

N

N

V

V

A

F

E

K

L

L

E

T

Q

P

V

A

W

W

Q

S

Y

Y

Q

S

T

F

G

G

D

D

V

E

K

K

L

-

P

-

Q

Q

D

R

V

G

T

Q

E
|
E

T

S

T

Q

Y

A

Q

I

V

V

T

S

P

-

L

-

K

-

V

-

D

D

D

G

R

V

L

I

Y

Y

I

V

C

T

T

A

P

S

H

Y

N

S

L

R

A

L

I

F

A

A

L

L

D

D

A

A

A

K

T

T

G

G

K

K

E

R

A

L

W

W

R

T

F

Y

D

N

A

H

N

R

S

L

G

-

L

-

E

-

P

P

D

D

R

D

Q

I

H

R

Q

P

T
x
C

C
|
C

R

-

G

-

V

-

T

-

Y

-

W

-

R

-

D

D

P

V

A

V

R

N

A
x
R

E

G

G

A

E

A

L

I

C

Y

A

G

E

D

R

K

V

V

Y

F

L

F

P

G

T

T

A

L

D

D

A

A

R

S

L

V

I

V

A

A

L

L

D

N

A

K

R

N

S

T

G

G

A

K

V

V

C

V

-

W

-

K

T

K

F

K

F

F

A

A

D

D

E

H

G

G

T

A

L

G

H

Y

L

T

E

M

S
x
T

G

G

M

A

P

P

Y

-

T

-

P

-

E

-

G

-

F

-

Y

-

S

T

T

I

S

V

P

K

P

D

V

G

A

K

V

T

G

G

G

K

R

V

I

L

I

I

G
x
H

G
|
G

A
x
S

V

S

N

G

D

D

N
x
E

F

F

S

G

V

V

F

V

S

-

Q

-

S

-

G

G

V

R

I

L

R

F

A

A

F

R

D

D

A

P

N

D

T

T

G

G

A

E

L

E

L

I

W

W

N

M

W

-

D

-

S

-

G

-

N

R

P

P

D

F

E

V

T

E

A

G

P

H

I

M

D

G

W

R

M

L

N

N

G

G

E

K

T

D

Y

S

T

T

A

V

-

T

-

G

-

D

-

V

-

K

-

A

-

P

-

S

-

W

-

P

-

D

-

R

N

N

S

S

P

P

N

T

S

G

-

K

-

V

-

E

-

S

-

W

-

S

-

H

-

G

-

A

-

P

W

W

S

Q

V

S

F

A

S

S

V

F

D

D

E

A

E

E

R

T

G

N

L

T

V

I

Y

I

I

V

P

G

L

A

G

G

N
|
N

Q

P

V

G

P

P

D

W

Q

N

L

T

G

W

F

A

D

R

R

T

S

A

P

K

A

G

V

G

E

N

E

P

H

H

-

D

-

Y

-

D

-

S

-

L

-

Y

S

T

S

S

S

G

V

Q

V

V

A

G

L

V

D

D

V

P

T

S

G

G

Q

E

K

V

A

K

W

W

V

F

F

Y

Q

Q

T

H

V

T

H

P

H

N

D

D

L

A

W

W

D
|
D

M

F

D

S

V

G

P

N

A

N

Q

E

P

L

V

V

L

L

I

F

D

D

L

Y

D

K

I

A

-

K

D

D

G

G

Q

K

T

I

V

V

P

K

A

A

L

T

V

A

Q

H

P

A

T

D

K
x
R

Q

N

G

G

D

F

I

F

Y

Y

V

V

L

V

N

D

R

R

E

S

T

N

G

G

E

K

E95 (= E207) binding pyrroloquinoline quinone
C139 (≠ T258) modified: Disulfide link with 140
CC 139:140 (≠ TC 258:259) mutation to AA: 15-fold decrease in catalytic activity with the natural electron acceptor cytochrome c550. Does not affect, or even increases, catalytic activity with artificial electron acceptors. Shows high decreased affinity for primary alcohols, while the affinity for the secondary alcohol 2-propanol is unaltered.
C140 (= C259) modified: Disulfide link with 139
R145 (≠ A271) binding pyrroloquinoline quinone
T189 (≠ S313) binding pyrroloquinoline quinone
HGS 207:209 (≠ GGA 339:341) binding pyrroloquinoline quinone
E213 (≠ N345) binding Ca(2+)
N300 (= N414) binding Ca(2+)
D350 (= D458) binding Ca(2+)
R378 (≠ K485) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:34 signal peptide
45 binding Ca(2+)
48 binding Ca(2+)
51 binding Ca(2+)
523 binding pyrroloquinoline quinone
587 binding pyrroloquinoline quinone

Query Sequence

>WP_069333644.1 NCBI__GCF_003046325.1:WP_069333644.1
MTATLTAIVLALAGLGLLIPGAWLAVLGGSWFYLVAGALMLLTGWQVFRRSSSAEWIYGG
LILGALVWSVWEVGFDWWELIPRGGIIAVLGIWLYLPFMRRQLLTEDGHPAPGLPLALPL
LAAIGVAIWSWTGDEAGHAGSLPTEVVSAEPDLGPTAPPGEWHQYGRTQYGQRYSPLEQI
NIQNVAELEQVWQYQTGDVKLPQDVTETTYQVTPLKVDDRLYICTPHNLAIALDAATGKE
AWRFDANSGLEPDRQHQTCRGVTYWRDPARAEGELCAERVYLPTADARLIALDARSGAVC
TFFADEGTLHLESGMPYTPEGFYYSTSPPVAVGGRIIIGGAVNDNFSVFSQSGVIRAFDA
NTGALLWNWDSGNPDETAPIDWMNGETYTANSPNSWSVFSVDEERGLVYIPLGNQVPDQL
GFDRSPAVEEHSSSVVALDVTTGQKAWVFQTVHHDLWDMDVPAQPVLIDLDIDGQTVPAL
VQPTKQGDIYVLNRETGEPILPVTEEPAPQEGGLPEESPAPTQPTSALSFKPAALREKDM
WGVTIYDQLACRIQYHRLNYEGRYTPPSLNGTIVYPGNFGTFNWGSVAVDPERQVMFGMP
TYLPFTVELVPAEDIPPPGANETASEQGLNRNEGAPYGVIMGPFLGPLGVPCSAPPWGYV
AGADLRTGDIAYMHRNGTVRDMTPLPLPLKVGVPGIGGPIVTRGGVAFLGAAVDDYLRAY
DVTTGEQLWQARLPAGGQSTPMTYEQDGRQFVVIVAGGHGSVGTKPGDYVIAYALPGGAE
G

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory