SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_069663041.1 NCBI__GCF_001730305.1:WP_069663041.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

1nsjA Crystal structure of phosphoribosyl anthranilate isomerase from thermotoga maritima (see paper)
38% identity, 99% coverage: 1:196/198 of query aligns to 2:203/205 of 1nsjA

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active site: C7 (= C6), D126 (≠ F118)
binding phosphate ion: G133 (= G127), V179 (= V173), S180 (= S174), S181 (= S175)

1lbmA Crystal structure of phosphoribosyl anthranilate isomerase (prai) in complex with reduced 1-(o-carboxyphenylamino)-1-deoxyribulose 5- phosphate (rcdrp) (see paper)
36% identity, 99% coverage: 1:196/198 of query aligns to 2:192/194 of 1lbmA

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1lbmA

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active site: C7 (= C6), D126 (≠ Q123)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: C7 (= C6), S34 (= S32), R36 (= R34), H83 (= H81), D126 (≠ Q123), G147 (= G152), G148 (= G153), D167 (= D172), S169 (= S174), S170 (= S175)

1piiA Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from escherichia coli refined at 2.0 angstroms resolution (see paper)
30% identity, 96% coverage: 4:193/198 of query aligns to 258:450/452 of 1piiA

query
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1piiA

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active site: C260 (= C6), D379 (≠ F118)
binding phosphate ion: G386 (= G130), G407 (= G153), N427 (≠ S174), S428 (= S175)

Sites not aligning to the query:

active site: 53, 55, 114, 163, 184, 214, 215
binding phosphate ion: 55, 215, 235, 236, 237

Query Sequence

>WP_069663041.1 NCBI__GCF_001730305.1:WP_069663041.1
MKVKICGLTTREQVDTAVKKGADYLGFVFAESKRAIRPEKVKEITKEVPKNVKKVGVFVT
PSNQEAETAIREAGLDMIQIHGKLVTRSYSVPVIQAIPVGSYEQEKMMKETTAEYLLFDA
LPQKFIGGNGKPFDWQQLNISKLKNKKIFIAGGLTSENVQEARATFSPYAVDVSSGVETN
GIKDLAKISEFLKKAKEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory