SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_072905141.1 NCBI__GCF_900142125.1:WP_072905141.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
50% identity, 97% coverage: 12:430/432 of query aligns to 7:424/427 of Q9S400

query
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Q9S400

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S21 (= S26) binding 3-phosphoshikimate
R25 (= R30) binding 3-phosphoshikimate
S166 (= S171) binding 3-phosphoshikimate
A167 (= A172) binding 3-phosphoshikimate
Q168 (= Q173) binding 3-phosphoshikimate
D312 (= D319) binding 3-phosphoshikimate
K339 (= K346) binding 3-phosphoshikimate

1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
49% identity, 97% coverage: 12:430/432 of query aligns to 7:424/427 of 1rf6A

query
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1rf6A

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active site: K20 (= K25), S21 (= S26), D47 (= D52), N90 (= N96), D115 (≠ Q121), R120 (= R126), D312 (= D319), E340 (= E347), H384 (= H391), R385 (= R392), T412 (= T418)
binding glyphosate: K20 (= K25), G92 (= G98), T93 (= T99), R120 (= R126), Q168 (= Q173), D312 (= D319), E340 (= E347), R343 (= R350), H384 (= H391), R385 (= R392)
binding shikimate-3-phosphate: S21 (= S26), R25 (= R30), S166 (= S171), Q168 (= Q173), R193 (= R198), I311 (= I318), D312 (= D319), K339 (= K346)

1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
49% identity, 97% coverage: 12:430/432 of query aligns to 7:424/427 of 1rf4A

query
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1rf4A

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V

V

T

E

I

L

E

D

D

R

T

A

G

E

C

A

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T

S

S

F

Y

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P

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F

W

F

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D

L

D

L

L

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S

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L

active site: K20 (= K25), S21 (= S26), D47 (= D52), N90 (= N96), D115 (≠ Q121), R120 (= R126), D312 (= D319), E340 (= E347), H384 (= H391), R385 (= R392), T412 (= T418)
binding (3r,4s,5r)-5-{[(1r)-1-carboxy-2-fluoro-1-(phosphonooxy)ethyl]oxy}-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K20 (= K25), S21 (= S26), R25 (= R30), G92 (= G98), T93 (= T99), R120 (= R126), S166 (= S171), A167 (= A172), Q168 (= Q173), R193 (= R198), D312 (= D319), K339 (= K346), E340 (= E347), R343 (= R350), H384 (= H391), R385 (= R392)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
50% identity, 97% coverage: 11:430/432 of query aligns to 7:425/438 of Q83E11

query
sites
Q83E11

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K21 (= K25) binding phosphoenolpyruvate
S22 (= S26) binding 3-phosphoshikimate
R26 (= R30) binding 3-phosphoshikimate
NSGT 93:96 (= NSGT 96:99) Phosphoenolpyruvate
G95 (= G98) binding phosphoenolpyruvate
T96 (= T99) binding phosphoenolpyruvate
R123 (= R126) binding phosphoenolpyruvate
S167 (= S171) binding 3-phosphoshikimate
A168 (= A172) binding 3-phosphoshikimate
Q169 (= Q173) binding 3-phosphoshikimate; binding phosphoenolpyruvate
D315 (= D319) binding 3-phosphoshikimate
K342 (= K346) binding 3-phosphoshikimate
R346 (= R350) binding phosphoenolpyruvate
R387 (= R392) binding phosphoenolpyruvate

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
50% identity, 97% coverage: 11:430/432 of query aligns to 9:427/440 of 3slhD

query
sites
3slhD

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active site: K23 (= K25), S24 (= S26), D50 (= D52), N95 (= N96), R125 (= R126), D317 (= D319), E345 (= E347), H388 (= H391), R389 (= R392), T415 (= T418)
binding glyphosate: K23 (= K25), G97 (= G98), T98 (= T99), R125 (= R126), Q171 (= Q173), D317 (= D319), E345 (= E347), R348 (= R350), H388 (= H391), R389 (= R392)
binding shikimate-3-phosphate: S24 (= S26), R28 (= R30), S169 (= S171), Q171 (= Q173), R196 (= R198), D317 (= D319), K344 (= K346)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S26), R28 (= R30), T98 (= T99), Q171 (= Q173), R196 (= R198), D317 (= D319), K344 (= K346)

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
50% identity, 97% coverage: 11:430/432 of query aligns to 9:423/434 of 4egrA

query
sites
4egrA

P

P

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S

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G

G

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K

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S

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H

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N

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R
|
R

I

I

A

A

M

M

C

V

D

D

T

G

L

L

G

Q

K

K

I

L

G

G

A

I

Q

A

I

A

E

E

P

S

L

L

E

P

D

D

G

G

M

V

R

I

V

I

T

Q

G

G

K

-

S

T

L

L

S

E

G

G

T

E

V

V

N

N

S

S

F

Y

G

D

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

A

M

F

A

A

V

V

A

A

A

G

L

T

R

L

A

A

S

K

G

G

E

P

V

V

T

R

I

I

E

R

D

N

T

C

G

D

C

N

T

V

S

K

T
|
T

S

S

F

F

P

P

N

N

F

F

W

V

E

E

L

L

L

A

E

N

N

E

I

V

active site: K23 (= K25), S24 (= S26), D50 (= D52), N95 (= N96), R125 (= R126), D313 (= D319), E341 (= E347), H384 (= H391), R385 (= R392), T411 (= T418)
binding phosphoenolpyruvate: K23 (= K25), G97 (= G98), T98 (= T99), R125 (= R126), D313 (= D319), E341 (= E347), R344 (= R350), R385 (= R392)

Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see paper)
47% identity, 97% coverage: 13:430/432 of query aligns to 16:443/455 of Q9R4E4

query
sites
Q9R4E4

T

S

S

G

V

L

K

S

G

G

E

T

I

V

V

R

V

I

P

P

G

G

D

D

K

K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

F

F

A

G

S

G

L

L

A

A

K

S

G

G

E

E

S

T

R

R

V

I

V

T

G

G

L

L

R

E

G

G

E

E

D

D

C

V

M

I

S

N

T

T

L

G

K

K

I

A

F

M

Q

Q

A

A

M

M

G

G

V

A

E

R

V

I

E

R

E

K

R

E

G

G

E

-

D

D

E

T

L

W

I

I

Q

D

G

G

R

V

G

G

I

N

D

G

G

G

L

L

E

A

P

P

D

E

D

A

V

P

L

L

D

D

C

F

G

G

N

N

S

A

G
x
A

T

T

T

G

M

C

R

R

L

L

M

T

S

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

Y

D

S

S

V

T

M

F

T

I

G

G

D

D

Q

A

Y

S

L

L

R

T

K

K

R

R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

R

R

E

E

M

M

G

G

A

V

K

Q

I

V

L

K

G

S

R

E

D

D

G

G

N

D

K

R

K

-

A

L

P

P

L

V

T

T

I

L

D

R

G

G

G

P

G

K

L

T

-

P

K

T

P

P

A

I

E

T

Y

Y

H

R

S

V

P

P

V

M

S

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

A

A

L

V

L

L

A

A

G

G

M

L

Q

N

I

T

E

P

G

G

T

I

T

T

V

V

Y

I

E

E

P

P

H

I

L

M

S

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

C

Q

Y

G

F

F

G

G

A

A

E

N

-

L

-

T

V

V

E

E

S

T

F

D

D

A

G

D

G

G

A

V

R

R

-

T

-

I

-

R

V

L

T

E

G

G

P

R

V

G

V

K

L

L

E

T

G

G

R

Q

E

V

V

I

F

D

V

V

P

P

G

G

D

D

I

P

S

S

A

T

A

A

F

F

F

P

L

L

V

V

A

A

G

A

L

L

I

L

V

V

P

P

D

G

S

S

E

D

L

V

L

T

L

I

K

L

N

N

V

V

G

L

V

M

N

N

P

P

T

T

R

R

S

T

G

G

I

L

I

I

D

L

I

T

L

L

Q

Q

Q

E

M

M

G

G

G

A

S

D

I

I

E

E

L

V

L

I

N

N

Q

P

R

R

E

L

L

A

S

G

G

G

E

E

P

D

V

V

A

A

D

D

L

L

L

R

V

V

K

R

S

S

Q

T

L

L

K

K

G

G

I

V

E

T

I

V

G

P

G

E

E

D

L

R

V

A

P

P

R

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

V

L

S

A

V

V

A

A

F

F

A

A

E

E

G

G

V

A

T

T

T

V

I

M

R

N

D

G

A

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E

E

T

S

D

D

R

R

I

L

A

S

A

A

M

V

C

A

D

N

T

G

L

L

G

K

K

L

I

N

G

G

A

V

Q

D

I

C

E

D

P

E

L

G

E

E

D

T

G

S

M

L

R

V

V

V

T

R

G

G

-

R

-

P

-

D

G

G

K

K

S

G

L

L

-

G

-

N

-

A

S

S

G

G

G

A

T

A

V

V

N

A

S

T

F

H

G

L

D

D

H

H

R

R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

A

G

L

L

R

V

A

S

S

E

G

N

E

P

V

V

T

T

I

V

E

D

D

D

T

A

G

T

C

M

T

I

S

A

T

T

S

S

F

F

P

P

N

E

F

F

W

M

E

D

L

L

L

M

E

A

N

G

I

L

S29 (= S26) binding 3-phosphoshikimate
R33 (= R30) binding 3-phosphoshikimate
A100 (≠ G98) mutation to G: Confers sensitivity to glyphosate allowing glyphosate to bind in its extended, inhibitory conformation.
S173 (= S171) binding 3-phosphoshikimate
A174 (= A172) binding 3-phosphoshikimate
Q175 (= Q173) binding 3-phosphoshikimate
D326 (= D319) binding 3-phosphoshikimate
K353 (= K346) binding 3-phosphoshikimate

2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
47% identity, 97% coverage: 13:430/432 of query aligns to 11:438/445 of 2pqcA

query
sites
2pqcA

T

S

S

G

V

L

K

S

G

G

E

T

I

V

V

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

F

F

A

G

S

G

L

L

A

A

K

S

G

G

E

E

S

T

R

R

V

I

V

T

G

G

L

L

R

E

G

G

E

E

D
|
D

C

V

M

I

S

N

T

T

L

G

K

K

I

A

F

M

Q

Q

A

A

M

M

G

G

V

A

E

R

V

I

E

R

E

K

R

E

G

G

E

-

D

D

E

T

L

W

I

I

Q

D

G

G

R

V

G

G

I

N

D

G

G

G

L

L

E

A

P

P

D

E

D

A

V

P

L

L

D

D

C

F

G

G

N
|
N

S

A

G

A

T
|
T

T

G

M

C

R

R

L

L

M

T

S

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

Y

D

S

S

V

T

M

F

T

I

G

G

D

D

Q

A

Y

S

L

L

R

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

R

R

E

E

M

M

G

G

A

V

K

Q

I

V

L

K

G

S

R

E

D

D

G

G

N

D

K

R

K

-

A

L

P

P

L

V

T

T

I

L

D

R

G

G

G

P

G

K

L

T

-

P

K

T

P

P

A

I

E

T

Y

Y

H

R

S

V

P

P

V

M

S

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

L

V

L

L

A

A

G

G

M

L

Q

N

I

T

E

P

G

G

T

I

T

T

V

V

Y

I

E

E

P

P

H

I

L

M

S

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

C

Q

Y

G

F

F

G

G

A

A

E

N

-

L

-

T

V

V

E

E

S

T

F

D

D

A

G

D

G

G

A

V

R

R

-

T

-

I

-

R

V

L

T

E

G

G

P

R

V

G

V

K

L

L

E

T

G

G

R

Q

E

V

V

I

F

D

V

V

P

P

G

G

D

D

I

P

S

S

A

T

A

A

F

F

F

P

L

L

V

V

A

A

G

A

L

L

I

L

V

V

P

P

D

G

S

S

E

D

L

V

L

T

L

I

K

L

N

N

V

V

G

L

V

M

N

N

P

P

T

T

R

R

S

T

G

G

I

L

I

I

D

L

I

T

L

L

Q

Q

Q

E

M

M

G

G

G

A

S

D

I

I

E

E

L

V

L

I

N

N

Q

P

R

R

E

L

L

A

S

G

G

G

E

E

P

D

V

V

A

A

D

D

L

L

L

R

V

V

K

R

S

S

Q

T

L

L

K

K

G

G

I

V

E

T

I

V

G

P

G

E

E

D

L

R

V

A

P

P

R

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

V

L

S

A

V

V

A

A

F

F

A

A

E

E

G

G

V

A

T

T

T

V

I

M

R

N

D

G

A

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E
|
E

T

S

D

D

R
|
R

I

L

A

S

A

A

M

V

C

A

D

N

T

G

L

L

G

K

K

L

I

N

G

G

A

V

Q

D

I

C

E

D

P

E

L

G

E

E

D

T

G

S

M

L

R

V

V

V

T

R

G

G

-

R

-

P

-

D

G

G

K

K

S

G

L

L

-

G

-

N

-

A

S

S

G

G

G

A

T

A

V

V

N

A

S

T

F

H

G

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

A

G

L

L

R

V

A

S

S

E

G

N

E

P

V

V

T

T

I

V

E

D

D

D

T

A

G

T

C

M

T

I

S

A

T
|
T

S

S

F

F

P

P

N

E

F

F

W

M

E

D

L

L

L

M

E

A

N

G

I

L

active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D319), E349 (= E347), H399 (= H391), R400 (= R392), T426 (= T418)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K25), S24 (= S26), R28 (= R30), T96 (= T99), R123 (= R126), S168 (= S171), Q170 (= Q173), D321 (= D319), K348 (= K346), E349 (= E347), R352 (= R350), R400 (= R392)

2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
47% identity, 97% coverage: 13:430/432 of query aligns to 11:438/445 of 2pqbA

query
sites
2pqbA

T

S

S

G

V

L

K

S

G

G

E

T

I

V

V

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

F

F

A

G

S

G

L

L

A

A

K

S

G

G

E

E

S

T

R

R

V

I

V

T

G

G

L

L

R

E

G

G

E

E

D
|
D

C

V

M

I

S

N

T

T

L

G

K

K

I

A

F

M

Q

Q

A

A

M

M

G

G

V

A

E

R

V

I

E

R

E

K

R

E

G

G

E

-

D

D

E

T

L

W

I

I

Q

D

G

G

R

V

G

G

I

N

D

G

G

G

L

L

E

A

P

P

D

E

D

A

V

P

L

L

D

D

C

F

G

G

N
|
N

S

A

G
x
A

T
|
T

T

G

M

C

R

R

L

L

M

T

S

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

Y

D

S

S

V

T

M

F

T

I

G

G

D

D

Q

A

Y

S

L

L

R

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

R

R

E

E

M

M

G

G

A

V

K

Q

I

V

L

K

G

S

R

E

D

D

G

G

N

D

K

R

K

-

A

L

P

P

L

V

T

T

I

L

D

R

G

G

G

P

G

K

L

T

-

P

K

T

P

P

A

I

E

T

Y

Y

H

R

S

V

P

P

V

M

S

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

L

V

L

L

A

A

G

G

M

L

Q

N

I

T

E

P

G

G

T

I

T

T

V

V

Y

I

E

E

P

P

H

I

L

M

S

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

C

Q

Y

G

F

F

G

G

A

A

E

N

-

L

-

T

V

V

E

E

S

T

F

D

D

A

G

D

G

G

A

V

R

R

-

T

-

I

-

R

V

L

T

E

G

G

P

R

V

G

V

K

L

L

E

T

G

G

R

Q

E

V

V

I

F

D

V

V

P

P

G

G

D

D

I

P

S

S

A

T

A

A

F

F

F

P

L

L

V

V

A

A

G

A

L

L

I

L

V

V

P

P

D

G

S

S

E

D

L

V

L

T

L

I

K

L

N

N

V

V

G

L

V

M

N

N

P

P

T

T

R

R

S

T

G

G

I

L

I

I

D

L

I

T

L

L

Q

Q

Q

E

M

M

G

G

G

A

S

D

I

I

E

E

L

V

L

I

N

N

Q

P

R

R

E

L

L

A

S

G

G

G

E

E

P

D

V

V

A

A

D

D

L

L

L

R

V

V

K

R

S

S

Q

T

L

L

K

K

G

G

I

V

E

T

I

V

G

P

G

E

E

D

L

R

V

A

P

P

R

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

V

L

S

A

V

V

A

A

F

F

A

A

E

E

G

G

V

A

T

T

T

V

I

M

R

N

D

G

A

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E
|
E

T

S

D

D

R
|
R

I

L

A

S

A

A

M

V

C

A

D

N

T

G

L

L

G

K

K

L

I

N

G

G

A

V

Q

D

I

C

E

D

P

E

L

G

E

E

D

T

G

S

M

L

R

V

V

V

T

R

G

G

-

R

-

P

-

D

G

G

K

K

S

G

L

L

-

G

-

N

-

A

S

S

G

G

G

A

T

A

V

V

N

A

S

T

F

H

G

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

A

G

L

L

R

V

A

S

S

E

G

N

E

P

V

V

T

T

I

V

E

D

D

D

T

A

G

T

C

M

T

I

S

A

T
|
T

S

S

F

F

P

P

N

E

F

F

W

M

E

D

L

L

L

M

E

A

N

G

I

L

active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D319), E349 (= E347), H399 (= H391), R400 (= R392), T426 (= T418)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K25), S24 (= S26), R28 (= R30), A95 (≠ G98), T96 (= T99), R123 (= R126), S168 (= S171), Q170 (= Q173), D321 (= D319), K348 (= K346), E349 (= E347), R352 (= R350), R400 (= R392)

2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
47% identity, 97% coverage: 13:430/432 of query aligns to 11:438/445 of 2ggaA

query
sites
2ggaA

T

S

S

G

V

L

K

S

G

G

E

T

I

V

V

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

F

F

A

G

S

G

L

L

A

A

K

S

G

G

E

E

S

T

R

R

V

I

V

T

G

G

L

L

R

E

G

G

E

E

D
|
D

C

V

M

I

S

N

T

T

L

G

K

K

I

A

F

M

Q

Q

A

A

M

M

G

G

V

A

E

R

V

I

E

R

E

K

R

E

G

G

E

-

D

D

E

T

L

W

I

I

Q

D

G

G

R

V

G

G

I

N

D

G

G

G

L

L

E

A

P

P

D

E

D

A

V

P

L

L

D

D

C

F

G

G

N
|
N

S
x
A

G
x
A

T
|
T

T

G

M

C

R

R

L

L

M

T

S

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

Y

D

S

S

V

T

M

F

T

I

G

G

D

D

Q

A

Y

S

L

L

R

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

R

R

E

E

M

M

G

G

A

V

K

Q

I

V

L

K

G

S

R

E

D

D

G

G

N

D

K

R

K

-

A

L

P

P

L

V

T

T

I

L

D

R

G

G

G

P

G

K

L

T

-

P

K

T

P

P

A

I

E

T

Y

Y

H

R

S

V

P

P

V

M

S

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

A

A

L

V

L

L

A

A

G

G

M

L

Q

N

I

T

E

P

G

G

T

I

T

T

V

V

Y

I

E

E

P

P

H

I

L

M

S

T

R
|
R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

C

Q

Y

G

F

F

G

G

A

A

E

N

-

L

-

T

V

V

E

E

S

T

F

D

D

A

G

D

G

G

A

V

R

R

-

T

-

I

-

R

V

L

T

E

G

G

P

R

V

G

V

K

L

L

E

T

G

G

R

Q

E

V

V

I

F

D

V

V

P

P

G

G

D

D

I

P

S

S

A

T

A

A

F

F

F

P

L

L

V

V

A

A

G

A

L

L

I

L

V

V

P

P

D

G

S

S

E

D

L

V

L

T

L

I

K

L

N

N

V

V

G

L

V

M

N

N

P

P

T

T

R

R

S

T

G

G

I

L

I

I

D

L

I

T

L

L

Q

Q

Q

E

M

M

G

G

G

A

S

D

I

I

E

E

L

V

L

I

N

N

Q

P

R

R

E

L

L

A

S

G

G

G

E

E

P

D

V

V

A

A

D

D

L

L

L

R

V

V

K

R

S

S

Q

T

L

L

K

K

G

G

I

V

E

T

I

V

G

P

G

E

E

D

L

R

V

A

P

P

R

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

V

L

S

A

V

V

A

A

F

F

A

A

E

E

G

G

V

A

T

T

T

V

I

M

R

N

D

G

A

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E
|
E

T

S

D

D

R
|
R

I

L

A

S

A

A

M

V

C

A

D

N

T

G

L

L

G

K

K

L

I

N

G

G

A

V

Q

D

I

C

E

D

P

E

L

G

E

E

D

T

G

S

M

L

R

V

V

V

T

R

G

G

-

R

-

P

-

D

G

G

K

K

S

G

L

L

-

G

-

N

-

A

S

S

G

G

G

A

T

A

V

V

N

A

S

T

F

H

G

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

A

G

L

L

R

V

A

S

S

E

G

N

E

P

V

V

T

T

I

V

E

D

D

D

T

A

G

T

C

M

T

I

S

A

T
|
T

S

S

F

F

P

P

N

E

F

F

W

M

E

D

L

L

L

M

E

A

N

G

I

L

active site: K23 (= K25), S24 (= S26), D50 (= D52), N93 (= N96), R123 (= R126), D321 (= D319), E349 (= E347), H399 (= H391), R400 (= R392), T426 (= T418)
binding glyphosate: K23 (= K25), A94 (≠ S97), A95 (≠ G98), T96 (= T99), R123 (= R126), D321 (= D319), E349 (= E347), R352 (= R350), R400 (= R392)
binding shikimate-3-phosphate: S24 (= S26), R28 (= R30), S168 (= S171), A169 (= A172), Q170 (= Q173), R195 (= R198), D321 (= D319), K348 (= K346)

2gg6A Cp4 epsp synthase liganded with s3p (see paper)
47% identity, 97% coverage: 13:430/432 of query aligns to 11:438/445 of 2gg6A

query
sites
2gg6A

T

S

S

G

V

L

K

S

G

G

E

T

I

V

V

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

F

F

A

G

S

G

L

L

A

A

K

S

G

G

E

E

S

T

R

R

V

I

V

T

G

G

L

L

R

E

G

G

E

E

D
|
D

C

V

M

I

S

N

T

T

L

G

K

K

I

A

F

M

Q

Q

A

A

M

M

G

G

V

A

E

R

V

I

E

R

E

K

R

E

G

G

E

-

D

D

E

T

L

W

I

I

Q

D

G

G

R

V

G

G

I

N

D

G

G

G

L

L

E

A

P

P

D

E

D

A

V

P

L

L

D

D

C

F

G

G

N
|
N

S

A

G

A

T
|
T

T

G

M

C

R

R

L

L

M

T

S

M

G

G

I

L

L

V

A

G

A</