SitesBLAST
Comparing WP_072908932.1 NCBI__GCF_900142125.1:WP_072908932.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
30% identity, 53% coverage: 385:832/838 of query aligns to 4:455/455 of 1wqaA
- active site: R11 (≠ K392), S101 (= S478), H102 (≠ P479), K111 (≠ I488), D243 (≠ G621), D245 (≠ S623), D247 (≠ E625), R248 (≠ K626), G330 (≠ R708), R340 (≠ F718)
- binding magnesium ion: S101 (= S478), D243 (≠ G621), D245 (≠ S623), D247 (≠ E625)
P74285 UTP--glucose-1-phosphate uridylyltransferase; Cyanobacterial UDP-glucose pyrophosphorylase; UDP-glucose pyrophosphorylase; UDP-Glc PPase; EC 2.7.7.9 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
33% identity, 37% coverage: 1:308/838 of query aligns to 1:322/388 of P74285
- A8 (≠ G8) mutation to G: Two-fold decrease in affinity for UTP. No effect on affinity for Glc-1P and on catalytic activity rate.
7whsA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gtp (see paper)
31% identity, 44% coverage: 1:365/838 of query aligns to 1:355/366 of 7whsA
- binding guanosine-5'-triphosphate: L6 (≠ M6), V7 (≠ A7), G8 (= G8), G9 (= G9), G11 (= G11), R13 (= R13), A52 (≠ L52), G85 (= G84), N108 (≠ S104), D110 (= D106)
- binding magnesium ion: D110 (= D106), D218 (= D217)
7x8kA Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
30% identity, 44% coverage: 1:370/838 of query aligns to 1:354/365 of 7x8kA
- binding guanosine-5'-diphosphate-alpha-d-mannose: V7 (≠ A7), G8 (= G8), E80 (≠ P79), G85 (= G84), P89 (≠ A88), N109 (≠ S104), D111 (= D106), G146 (= G141), E162 (= E156), N172 (= N169), G174 (= G171), E194 (≠ S192)
7x8kB Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
30% identity, 44% coverage: 1:370/838 of query aligns to 1:355/367 of 7x8kB
O22287 Mannose-1-phosphate guanylyltransferase 1; GDP-mannose pyrophosphorylase 1; Protein CYTOKINESIS DEFECTIVE 1; Protein EMBRYO DEFECTIVE 101; Protein HYPERSENSITIVE TO AMMONIUM ION 1; Protein SENSITIVE TO OZONE 1; Protein VITAMIN C DEFECTIVE 1; EC 2.7.7.13 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
30% identity, 44% coverage: 1:370/838 of query aligns to 1:355/361 of O22287
- L6 (≠ M6) binding GDP-alpha-D-mannose
- V7 (≠ A7) binding GDP-alpha-D-mannose
- G9 (= G9) binding diphosphate
- G11 (= G11) binding diphosphate; mutation to S: In hsn1; reduced enzyme activity, ascorbate concentrations and N-glycosylation, and increased sensitivity to ammonium.
- T12 (= T12) binding diphosphate
- R13 (= R13) binding diphosphate
- P22 (= P22) mutation to S: In vtc1-1 and vtc1-2; reduced enzyme activity and ascorbate concentrations, and ozone-sensitive.
- K23 (= K23) binding diphosphate
- D27 (≠ P27) mutation to E: Abolishes interaction with CSN5B and subsequent degradation in the dark by the 26S proteasome, and increases ascorbate accumulation in seedlings.
- G85 (= G84) binding GDP-alpha-D-mannose
- P89 (≠ A88) mutation to L: In cyt1-1; deficient in N-glycosylation and cellulose, and embryo lethal.
- N109 (≠ S104) binding GDP-alpha-D-mannose
- D111 (= D106) binding GDP-alpha-D-mannose
- G146 (= G141) binding GDP-alpha-D-mannose
- N173 (= N169) binding GDP-alpha-D-mannose
Sites not aligning to the query:
- 223:361 mutation Missing: Reduces catalytic activity 3-fold.
7d73E Cryo-em structure of gmppa/gmppb complex bound to gtp (state i) (see paper)
29% identity, 44% coverage: 1:368/838 of query aligns to 1:352/360 of 7d73E
- binding guanosine-5'-triphosphate: L6 (≠ M6), V7 (≠ A7), G8 (= G8), G9 (= G9), G11 (= G11), T12 (= T12), R13 (= R13), A52 (≠ L52), S54 (≠ Y54), E80 (≠ P79), P83 (≠ D82), G85 (= G84), N108 (≠ S104)
7d72K Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
29% identity, 44% coverage: 1:368/838 of query aligns to 1:352/360 of 7d72K
- binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (≠ M6), V7 (≠ A7), G8 (= G8), G85 (= G84), T86 (= T85), N108 (≠ S104), S109 (≠ G105), D110 (= D106), N172 (= N169), E194 (≠ S192), D218 (= D217)
7d72E Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
29% identity, 44% coverage: 1:368/838 of query aligns to 1:352/360 of 7d72E
Q9Y5P6 Mannose-1-phosphate guanylyltransferase catalytic subunit beta; GDP-mannose pyrophosphorylase B; GTP-mannose-1-phosphate guanylyltransferase beta; EC 2.7.7.13 from Homo sapiens (Human) (see 7 papers)
29% identity, 44% coverage: 1:368/838 of query aligns to 1:352/360 of Q9Y5P6
- 2:222 (vs. 2:221, 35% identical) Substrate-binding domain
- P22 (= P22) to S: in MDDGC14; causes protein aggregation; reduces catalytic activity; dbSNP:rs397509424
- D27 (≠ P27) to H: in MDDGC14; the protein remains distributed in the cytoplasm and has no discernable changes compared to wild-type; reduces catalytic activity; dbSNP:rs142336618
- P32 (= P32) to L: in MDDGB14; causes protein aggregation; reduces catalytic activity; fails to rescue phenotype when expressed in a zebrafish disease model; dbSNP:rs397509426
- D110 (= D106) binding GDP-alpha-D-mannose; binding Mg(2+)
- Q184 (≠ D181) to R: in dbSNP:rs1466685
- R185 (≠ L182) to C: in MDDGB14; the protein remains distributed in the cytoplasm and has no discernable changes compared to wild-type; dbSNP:rs397509425
- I193 (≠ W191) mutation to T: Reduces enzymatic activity.
- D218 (= D217) binding GDP-alpha-D-mannose; binding Mg(2+); mutation to A: Reduces GDP-alpha-D-mannose binding affinity and inhibits catalytic activity but does not affect assembly of GMPPA-GMPPB complex. Does not rescue the knockdown phenotype in a zebrafish disease model.; mutation to Q: Abrogates enzyme activity.
- I219 (= I218) to T: in MDDGC14; reduces catalytic activity; dbSNP:rs761714818
- C266 (= C287) mutation to Y: Reduces interaction with GMPPB but not with GMPPA.
- R287 (≠ E302) to Q: in MDDGB14 and MDDGC14; dbSNP:rs202160208; mutation to E: Disrupts interaction with other GMPPB molecules but not with GMPPA.
- R293 (≠ D308) to W: in MDDGC14; slight reduction in protein abundance; reduces interaction with GMPPB but not with GMPPA; dbSNP:rs756682220
- L303 (≠ I318) mutation to F: Reduces interaction with GMPPB but not with GMPPA.
- N322 (≠ Q337) to K: in MDDGC14; no change in protein abundance; dbSNP:rs781114909
- V330 (≠ S345) to I: in MDDGC14; causes protein aggregation; dbSNP:rs199922550
- D334 (≠ K349) to N: in MDDGA14; causes protein aggregation; reduces interaction with GMPPB but not with GMPPA; dbSNP:rs397509422
- E335 (= E350) mutation to R: Disrupted interaction with GMPPA and other GMPPB molecules.
- G340 (≠ A355) to R: in MDDGC14; slight reduction in protein abundance; shows an increased propensity to form punctate aggregates; dbSNP:rs1064796834
- LPHK 344:347 (≠ WPSK 360:363) mutation to AAAA: Does not disrupt the interaction with GMPPA or other GMPPB molecules.
Sites not aligning to the query:
- 245:360 Hexapeptide repeat domain
- 357 R → H: in MDDGC14; no change in protein abundance; shows an increased propensity to form punctate aggregates; reduces interaction with GMPPA but not with GMPPB; dbSNP:rs771861177
- 358:360 mutation Missing: Reduced efficiency of allosteric inhibition by GMPPA but interaction with GMPPA or other GMPPB molecules is not disrupted.
7whtA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gdp-mannose (see paper)
30% identity, 44% coverage: 1:365/838 of query aligns to 1:349/360 of 7whtA