SitesBLAST

VDAPLL 278:283 (≠ VDKPIV 15:20) binding
N295 (= N32) mutation to A: Reduces the binding of L-methionine to undetectable levels.
NI 295:296 (= NI 32:33) binding

Sites not aligning to the query:

40:46 binding
166 E→Q: Exhibits little ATPase activity.

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 47% coverage: 5:72/146 of query aligns to 269:339/344 of 6cvlD

query
sites
6cvlD

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E

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19, 41, 42, 43, 44, 45, 46, 47, 141, 143, 146, 200

2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
45% identity, 29% coverage: 90:131/146 of query aligns to 143:184/337 of 2ivfB

query
sites
2ivfB

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binding fe3-s4 cluster: C148 (= C95), T150 (= T97), I153 (≠ L100), C169 (= C116), K170 (≠ S117), G171 (≠ A118), H172 (≠ C119), R173 (≠ E120), H174 (≠ L121), C175 (= C122)
binding protoporphyrin ix containing fe: T150 (= T97), K170 (≠ S117), H172 (≠ C119)
binding iron/sulfur cluster: C144 (= C91), V162 (= V109), C179 (= C126), A183 (= A130), I184 (≠ M131)

Sites not aligning to the query:

binding fe3-s4 cluster: 193
binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41, 135, 136, 137, 138, 139, 142, 195, 196, 197, 198, 199, 209, 211, 215, 219, 220

8a8oD Paps reductase from methanothermococcus thermolithotrophicus refined to 1.45 a (see paper)
39% identity, 37% coverage: 78:131/146 of query aligns to 2:55/102 of 8a8oD

query
sites
8a8oD

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binding iron/sulfur cluster: I2 (= I78), C9 (= C85), I10 (≠ Y86), G11 (≠ Q87), C12 (= C88), G13 (= G89), C15 (= C91), C19 (= C95), P20 (= P96), S32 (≠ A108), C40 (= C116), W41 (≠ S117), D42 (≠ A118), C43 (= C119), A44 (≠ E120), C46 (= C122), C50 (= C126), I55 (≠ M131)

8f61B Dihydropyrimidine dehydrogenase (dpd) c671s mutant soaked with dihydrothymine quasi-anaerobically (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 925:1010/1016 of 8f61B

query
sites
8f61B

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binding iron/sulfur cluster: A944 (≠ Q76), C951 (= C85), I952 (≠ Y86), N953 (≠ Q87), C954 (= C88), G955 (= G89), C957 (= C91), C961 (= C95), Y966 (≠ A99), I969 (= I102), P978 (≠ L110), C984 (= C116), T985 (≠ S117), G986 (≠ A118), C987 (= C119), T988 (≠ E120), C990 (= C122), C994 (= C126), P995 (= P127), C999 (≠ M131), I1000 (= I132)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 229, 233, 258, 259, 281, 341, 344, 478, 479, 486, 487, 490
binding flavin mononucleotide: 548, 549, 572, 573, 607, 666, 707, 734, 764, 765, 791, 792, 793, 814, 815
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 93, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154, 488
binding (5S)-5-methyl-1,3-diazinane-2,4-dione: 607, 609, 734, 735

7m32A Dihydropyrimidine dehydrogenase (dpd) c671a mutant soaked with uracil and NADPH anaerobically (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 919:1004/1011 of 7m32A

query
sites
7m32A

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binding iron/sulfur cluster: A938 (≠ Q76), C945 (= C85), I946 (≠ Y86), N947 (≠ Q87), C948 (= C88), G949 (= G89), C951 (= C91), C955 (= C95), I963 (= I102), P972 (≠ L110), C978 (= C116), T979 (≠ S117), G980 (≠ A118), C981 (= C119), T982 (≠ E120), C984 (= C122), C988 (= C126), P989 (= P127), I994 (= I132)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 233, 258, 259, 281, 282, 340, 341, 478, 479, 486, 487, 490
binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: 548, 549, 572, 573, 588, 607, 666, 701, 758, 759, 786, 787, 808, 809, 812
binding leucine: 577, 615
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154
binding uracil: 607, 609, 728, 729

1h7xA Dihydropyrimidine dehydrogenase (dpd) from pig, ternary complex of a mutant enzyme (c671a), NADPH and 5-fluorouracil (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 926:1011/1016 of 1h7xA

query
sites
1h7xA

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binding iron/sulfur cluster: A945 (≠ Q76), C952 (= C85), I953 (≠ Y86), N954 (≠ Q87), C955 (= C88), G956 (= G89), C958 (= C91), C962 (= C95), I970 (= I102), C985 (= C116), T986 (≠ S117), G987 (≠ A118), C988 (= C119), T989 (≠ E120), C991 (= C122), C995 (= C126), P996 (= P127), I1001 (= I132)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 673, 708, 734
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 234, 259, 260, 282, 283, 285, 342, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 708, 765, 766, 792, 793, 794, 815, 816, 819
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 338, 339, 342, 363, 364, 370, 436, 437, 480, 483, 484, 485, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 134, 135, 139, 140, 141, 149, 151, 155
binding 5-fluorouracil: 608, 610, 735, 736

7m31A Dihydropyrimidine dehydrogenase (dpd) c671s mutant soaked with thymine and NADPH anaerobically (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 921:1006/1011 of 7m31A

query
sites
7m31A

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binding iron/sulfur cluster: A940 (≠ Q76), C947 (= C85), I948 (≠ Y86), N949 (≠ Q87), C950 (= C88), G951 (= G89), C953 (= C91), C957 (= C95), I965 (= I102), P974 (≠ L110), C980 (= C116), T981 (≠ S117), G982 (≠ A118), C983 (= C119), T984 (≠ E120), C986 (= C122), C990 (= C126), P991 (= P127), C995 (≠ M131), I996 (= I132)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 228, 229, 233, 259, 281, 341, 478, 479, 486, 487, 490
binding flavin mononucleotide: 548, 549, 572, 573, 588, 607, 666, 703, 760, 761, 787, 788, 789, 810, 811, 814
binding nadp nicotinamide-adenine-dinucleotide phosphate: 338, 339, 340, 341, 362, 363, 435, 436, 437, 485
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 93, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154
binding thymine: 607, 610, 730, 731

7ljuA Porcine dihydropyrimidine dehydrogenase (dpd) crosslinked with 5- ethynyluracil (5eu) (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 926:1011/1016 of 7ljuA

query
sites
7ljuA

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binding iron/sulfur cluster: C952 (= C85), I953 (≠ Y86), N954 (≠ Q87), C955 (= C88), G956 (= G89), K957 (≠ A90), C958 (= C91), C962 (= C95), I970 (= I102), P979 (≠ L110), C985 (= C116), T986 (≠ S117), G987 (≠ A118), C988 (= C119), T989 (≠ E120), C991 (= C122), C995 (= C126), P996 (= P127), C1000 (≠ M131), I1001 (= I132)

Sites not aligning to the query:

active site: 573, 670, 708
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 230, 234, 260, 282, 342, 479, 480, 487, 488, 491
binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: 549, 550, 573, 574, 589, 608, 667, 708, 765, 766, 792, 793, 794, 815, 816, 819
binding nadp nicotinamide-adenine-dinucleotide phosphate: 339, 340, 341, 342, 363, 364, 370, 436, 437, 438, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 134, 135, 139, 140, 141, 149, 151, 155
binding 5-ethynylpyrimidine-2,4(1H,3H)-dione: 608, 612, 667, 735, 736

7ljtA Porcine dihydropyrimidine dehydrogenase (dpd) soaked with 5- ethynyluracil (5eu), NADPH - 20 minutes (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 926:1011/1016 of 7ljtA

query
sites
7ljtA

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binding iron/sulfur cluster: C952 (= C85), I953 (≠ Y86), C955 (= C88), G956 (= G89), C958 (= C91), C962 (= C95), I970 (= I102), P979 (≠ L110), C985 (= C116), T986 (≠ S117), G987 (≠ A118), C988 (= C119), T989 (≠ E120), C991 (= C122), C995 (= C126), P996 (= P127), C1000 (≠ M131), I1001 (= I132)

Sites not aligning to the query:

active site: 573, 670, 708
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 229, 230, 234, 260, 282, 283, 342, 479, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 608, 667, 708, 735, 765, 766, 792, 793, 794, 815, 816
binding nadp nicotinamide-adenine-dinucleotide phosphate: 288, 339, 340, 341, 342, 363, 364, 370, 436, 437, 438, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 86, 90, 94, 96, 129, 135, 139, 140, 141, 149, 151, 155
binding 5-ethynylpyrimidine-2,4(1H,3H)-dione: 608, 611, 612, 667, 735, 736

7ljsA Porcine dihydropyrimidine dehydrogenase (dpd) complexed with 5- ethynyluracil (5eu) - open form (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 915:1000/1005 of 7ljsA

query
sites
7ljsA

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q

A

G

V

I

I

R

-

D

D

E

E

D

E

K

M

C
|
C

Y
x
I

Q

N

C
|
C

G
|
G

A

K

C
|
C

T

Y

A

M

V

T

C
|
C

-

N

P

D

T

S

G

G

A

Y

L

Q

H

A

I
|
I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L
x
P

F

T

E

V

S

T

E

D

R

T

C
|
C

S
x
T

A
x
G

C
|
C

E
x
T

L

L

C
|
C

V

L

K

S

T

V

C
|
C

P
|
P

A

I

R

I

A

D

M
x
C

I

I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

binding iron/sulfur cluster: C941 (= C85), I942 (≠ Y86), C944 (= C88), G945 (= G89), C947 (= C91), C951 (= C95), I959 (= I102), P968 (≠ L110), C974 (= C116), T975 (≠ S117), G976 (≠ A118), C977 (= C119), T978 (≠ E120), C980 (= C122), C984 (= C126), P985 (= P127), C989 (≠ M131)

Sites not aligning to the query:

active site: 573, 670, 703
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 230, 234, 260, 282, 341, 342, 479, 480, 486, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 608, 667, 703, 760, 761, 787, 788, 789, 810, 811
binding iron/sulfur cluster: 78, 80, 81, 86, 90, 94, 96, 129, 135, 139, 140, 141, 149, 155
binding 5-ethynylpyrimidine-2,4(1H,3H)-dione: 608, 611, 612, 730, 731

1gthD Dihydropyrimidine dehydrogenase (dpd) from pig, ternary complex with NADPH and 5-iodouracil (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 926:1011/1019 of 1gthD

query
sites
1gthD

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q
x
A

G

V

I
|
I

R

-

D

D

E

E

D

E

K

M

C
|
C

Y
x
I

Q
x
N

C
|
C

G
|
G

A
x
K

C
|
C

T

Y

A

M

V

T

C
|
C

-

N

P

D

T

S

G

G

A

Y

L

Q

H

A

I
|
I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L

P

F

T

E

V

S

T

E

D

R

T

C
|
C

S
x
T

A
x
G

C
|
C

E
x
T

L

L

C
|
C

V

L

K

S

T

V

C
|
C

P
|
P

A

I

R

I

A

D

M
x
C

I
|
I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

binding iron/sulfur cluster: A945 (≠ Q76), I947 (= I78), C952 (= C85), I953 (≠ Y86), N954 (≠ Q87), C955 (= C88), G956 (= G89), K957 (≠ A90), C958 (= C91), C962 (= C95), I970 (= I102), C985 (= C116), T986 (≠ S117), G987 (≠ A118), C988 (= C119), T989 (≠ E120), C991 (= C122), C995 (= C126), P996 (= P127), C1000 (≠ M131), I1001 (= I132)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 673, 708, 734
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 229, 230, 234, 259, 260, 281, 282, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 708, 765, 766, 792, 793, 794, 815, 816, 819
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 130, 338, 339, 340, 341, 342, 363, 364, 370, 436, 437, 438, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 131, 134, 135, 139, 140, 141, 149, 151, 151, 155
binding uracil: 608, 610, 667, 670, 671, 735, 736

1gthA Dihydropyrimidine dehydrogenase (dpd) from pig, ternary complex with NADPH and 5-iodouracil (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 926:1011/1019 of 1gthA

query
sites
1gthA

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q

A

G

V

I
|
I

R

-

D

D

E

E

D

E

K

M

C
|
C

Y
x
I

Q
x
N

C
|
C

G
|
G

A

K

C
|
C

T

Y

A

M

V

T

C
|
C

-

N

P

D

T

S

G

G

A

Y

L

Q

H

A

I
|
I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L
x
P

F

T

E

V

S

T

E

D

R

T

C
|
C

S
x
T

A
x
G

C
|
C

E
x
T

L

L

C
|
C

V

L

K

S

T

V

C
|
C

P
|
P

A

I

R

I

A

D

M
x
C

I
|
I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

binding iron/sulfur cluster: I947 (= I78), C952 (= C85), I953 (≠ Y86), N954 (≠ Q87), C955 (= C88), G956 (= G89), C958 (= C91), C962 (= C95), I970 (= I102), P979 (≠ L110), C985 (= C116), T986 (≠ S117), G987 (≠ A118), C988 (= C119), T989 (≠ E120), C991 (= C122), C995 (= C126), P996 (= P127), C1000 (≠ M131), I1001 (= I132)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 673, 708, 734
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 229, 230, 234, 259, 260, 282, 342, 479, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 708, 736, 765, 766, 792, 793, 794, 815, 816, 819
binding (5s)-5-iododihydro-2,4(1h,3h)-pyrimidinedione: 608, 610, 667, 670, 672, 735, 736
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 130, 339, 340, 341, 342, 363, 364, 370, 436, 437, 438, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 125, 129, 131, 134, 135, 139, 140, 141, 149, 151, 151, 155

1gteA Dihydropyrimidine dehydrogenase (dpd) from pig, binary complex with 5- iodouracil (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 915:1000/1005 of 1gteA

query
sites
1gteA

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q
x
A

G

V

I
|
I

R

-

D

D

E

E

D

E

K

M

C
|
C

Y
x
I

Q
x
N

C
|
C

G
|
G

A
x
K

C
|
C

T

Y

A

M

V

T

C
|
C

-

N

P

D

T

S

G

G

A

Y

L

Q

H

A

I
|
I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L

P

F

T

E
x
V

S

T

E

D

R

T

C
|
C

S
x
T

A
x
G

C
|
C

E
x
T

L

L

C
|
C

V

L

K

S

T

V

C
|
C

P
|
P

A

I

R

I

A

D

M

C

I
|
I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

binding iron/sulfur cluster: A934 (≠ Q76), I936 (= I78), C941 (= C85), I942 (≠ Y86), N943 (≠ Q87), C944 (= C88), G945 (= G89), K946 (≠ A90), C947 (= C91), C951 (= C95), I959 (= I102), V970 (≠ E112), C974 (= C116), T975 (≠ S117), G976 (≠ A118), C977 (= C119), T978 (≠ E120), C980 (= C122), C984 (= C126), P985 (= P127), I990 (= I132)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 703, 729
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 234, 259, 260, 282, 283, 285, 341, 342, 479, 480, 486, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 703, 730, 760, 761, 787, 788, 789, 810, 811, 814
binding 5-iodouracil: 608, 611, 612, 667, 730, 731
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 131, 134, 135, 139, 140, 141, 149, 151, 155

1gt8A Dihydropyrimidine dehydrogenase (dpd) from pig, ternary complex with NADPH and uracil-4-acetic acid (see paper)
32% identity, 58% coverage: 59:142/146 of query aligns to 924:1009/1017 of 1gt8A

query
sites
1gt8A

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q

A

G

V

I

I

R

-

D

D

E

E

D

E

K

M

C
|
C

Y
x
I

Q
x
N

C
|
C

G
|
G

A
x
K

C
|
C

T

Y

A

M

V

T

C
|
C

-

N

P

D

T

S

G

G

A

Y

L

Q

H
x
A

I
|
I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L
x
P

F

T

E
x
V

S

T

E

D

R

T

C
|
C

S
x
T

A
x
G

C
|
C

E
x
T

L

L

C
|
C

V

L

K

S

T

V

C
|
C

P
|
P

A

I

R

I

A

D

M

C

I
|
I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

binding iron/sulfur cluster: C950 (= C85), I951 (≠ Y86), N952 (≠ Q87), C953 (= C88), G954 (= G89), K955 (≠ A90), C956 (= C91), C960 (= C95), A967 (≠ H101), I968 (= I102), P977 (≠ L110), V979 (≠ E112), C983 (= C116), T984 (≠ S117), G985 (≠ A118), C986 (= C119), T987 (≠ E120), C989 (= C122), C993 (= C126), P994 (= P127), I999 (= I132)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 673, 706, 732
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 229, 230, 234, 259, 260, 282, 342, 479, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 608, 667, 706, 734, 763, 764, 790, 791, 813, 814
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 130, 339, 340, 341, 342, 363, 370, 436, 437, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 134, 135, 139, 140, 149, 151, 155, 489
binding uracil-6-acetic acid: 608, 610, 611, 612, 733, 761

Q28943 Dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; EC 1.3.1.2 from Sus scrofa (Pig) (see 3 papers)
32% identity, 58% coverage: 59:142/146 of query aligns to 927:1012/1025 of Q28943

query
sites
Q28943

K

K

G

A

L

L

K

Q

Y

Y

L

L

K

G

D

T

I

F

G

G

-

E

-

L

V

S

K

N

V

I

E

E

S

Q

I

V

A

V

Q

A

G

V

I

I

R

-

D

D

E

E

D

E

K

M

C

C

Y

I

Q

N

C

C

G

G

A

K

C

C

T

Y

A

M

V

T

C

C

-

N

P

D

T

S

G

G

A

Y

L

Q

H

A

I

I

R

Q

-

F

R

D

P

P

E

E

M

T

A

H

V

L

L

P

F

T

E

V

S

T

E

D

R

T

C

C

S

T

A

G

C

C

E

T

L

L

C

C

V

L

K

S

T

V

C

C

P

P

A

I

R

I

A

D

M

C

I

I

V

R

M

M

F

V

D

S

R

R

K

T

V

T

E

P

L

Y

E

E

Sites not aligning to the query:

126 C→A: No effect on enzyme activity. Reduced iron content.
129 binding
156 Q→E: Loss of enzyme activity. Reduces iron content.
194:198 binding
218:226 binding
235 binding ; mutation R->A,K: Loss of enzyme activity. Loss of FAD binding.
261 binding
340:343 binding
364:365 binding
371 binding
437:439 binding
480:489 binding
481:487 binding
550 binding
574:575 binding
670 S→A: Strongly reduced affinity for uracil. Reduces enzyme activity by 30%.
671 active site, Proton acceptor; C→A: Reduces catalytic activity by 99%.
709 binding
767 binding
793:795 binding
816:817 binding

5t61L Tungsten formylmethanofuran dehydrogenase subunit fwdF (see paper)
31% identity, 49% coverage: 74:145/146 of query aligns to 182:269/348 of 5t61L

query
sites
5t61L

I

V

A

A

Q

T

G

D

I
|
I

R

R

R

V

D

D

E

E

D

D

K

K

C
|
C

Y

V

Q
x
H

C
|
C

G
|
G

A

I

C
|
C

T

K

A

R

V

I

C
|
C

P
|
P

T

V

G

D

A

A

L
x
I

-

M

-

Q

-

V

-
x
C

-

R

-

I

-
x
C

-

P

-

Y

-

G

-

E

-

Y

H

E

I

I

R

K

R

T

P

P

E

E

M

V

-

T

-

G

A

T

V

S

L

Y

F

I

E

D

S

P

E

E

R

L

C
|
C

S
x
V

A
x
N

C
|
C

E
x
G

L

W

C
|
C

V

Q

K

E

T

I

C
|
C

P
|
P

A

V

R

D

A

A

M

A

I

T

V

V

M

T

-

K

-

P

F

F

D

E

R

G

K

E

V

L

E

I

L

I

E

D

E

Q

D

D

V

T

binding iron/sulfur cluster: I186 (= I78), C193 (= C85), H195 (≠ Q87), C196 (= C88), G197 (= G89), C199 (= C91), C203 (= C95), P204 (= P96), I208 (≠ L100), C212 (vs. gap), C215 (vs. gap), C238 (= C116), V239 (≠ S117), N240 (≠ A118), C241 (= C119), G242 (≠ E120), C244 (= C122), C248 (= C126), P249 (= P127)

Sites not aligning to the query:

binding iron/sulfur cluster: 30, 31, 32, 33, 34, 36, 40, 41, 70, 71, 72, 73, 74, 76, 80, 85, 114, 117, 118, 120, 124, 129, 146, 153, 156, 159, 163, 164, 168, 270, 272, 273, 274, 276, 280, 281, 284, 307, 308, 309, 310, 311, 313, 317

3egwB The crystal structure of the narghi mutant narh - c16a
23% identity, 53% coverage: 58:135/146 of query aligns to 139:236/509 of 3egwB

query
sites
3egwB

E

E

K

K

G

G

L

P

K

N

Y

W

L

E

K

D

D

D

I

L

G

G

V

G

K

E

V

F

E

D

S

K

I

L

A

A

Q

K

G

D

I

K

R

N

R

F

D

D

E

N

D

I

K

Q

-

K

-

A

-

M

-

Y

-

S

-

Q

-

F

-

E

-

N

-

T

-

F

-

M

-

Y

-

L

-
x
P

-

R

-

L

C
|
C

Y
x
E

Q
x
H

C
|
C

-

L

-

N

G
x
P

A

A

C
|
C

T

V

A

A

V

T

C
|
C

P

P

T
x
S

G

G

A

A

L
x
I

H

Y

I

K

R

R

R

E

P

E

E

D

M

G

A

I

V

V

L

L

F

I

E

D

S

Q

E

D

R

K

C
|
C

S
x
R

A
x
G

C
x
W

E
x
R

L

M

C
|
C

V

I

K

T

T

G

C
|
C

P

P

A

Y

R

K

A

K

M
x
I

I

Y

V

F

M

N

F

W

binding fe3-s4 cluster: P181 (vs. gap), C196 (= C95), S198 (≠ T97), I201 (≠ L100), C217 (= C116), R218 (≠ S117), G219 (≠ A118), W220 (≠ C119), R221 (≠ E120), C223 (= C122)
binding protoporphyrin ix containing fe: W220 (≠ C119), R221 (≠ E120)
binding iron/sulfur cluster: C184 (= C85), E185 (≠ Y86), H186 (≠ Q87), C187 (= C88), P190 (≠ G89), C192 (= C91), C227 (= C126), I232 (≠ M131)

Sites not aligning to the query:

binding fe3-s4 cluster: 17, 18, 19, 20, 22, 241, 263, 265, 268
binding protoporphyrin ix containing fe: 88, 89
binding iron/sulfur cluster: 26, 30, 41, 42, 243, 244, 246, 247, 257, 259

Query Sequence

>WP_073036654.1 NCBI__GCF_900129305.1:WP_073036654.1
MFARMLVLRFPKEVVDKPIVTNLVRNYNLSFNILKAQIFPRKEGLMVLELRGNRKDYEKG
LKYLKDIGVKVESIAQGIRRDEDKCYQCGACTAVCPTGALHIRRPEMAVLFESERCSACE
LCVKTCPARAMIVMFDRKVELEEDVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory