SitesBLAST
Comparing WP_073037802.1 NCBI__GCF_900129305.1:WP_073037802.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
1aorA Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase (see paper)
49% identity, 100% coverage: 2:609/609 of query aligns to 3:603/605 of 1aorA
- binding fe (iii) ion: E332 (≠ K334), H383 (≠ V383)
- binding tungstopterin cofactor: R76 (= R75), A92 (≠ S91), N93 (= N92), G95 (= G94), R182 (= R180), A183 (= A181), G185 (= G183), R186 (= R184), E311 (= E313), E313 (= E315), D338 (≠ N340), D343 (= D345), T344 (= T346), R444 (≠ I444), H448 (= H448), I449 (≠ M449), K450 (≠ R450), D489 (= D488), C494 (= C493), L495 (≠ V494), F496 (= F495)
- binding iron/sulfur cluster: R76 (= R75), C288 (= C287), C291 (= C290), I293 (= I292), C295 (= C294), C494 (= C493), F496 (= F495)
8c0zB Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
48% identity, 100% coverage: 1:608/609 of query aligns to 1:616/616 of 8c0zB
- binding magnesium ion: N92 (= N92), A182 (= A181)
- binding iron/sulfur cluster: R75 (= R75), C295 (= C287), C298 (= C290), C302 (= C294), C509 (= C493)
- binding tungsten cofactor: R75 (= R75), S91 (= S91), N92 (= N92), G94 (= G94), R181 (= R180), A182 (= A181), A183 (= A182), G184 (= G183), E329 (= E313), E331 (= E315), N356 (= N340), P362 (≠ T346), L465 (≠ M449), D504 (= D488), V510 (= V494), F511 (= F495)
8c0zA Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
48% identity, 100% coverage: 1:608/609 of query aligns to 1:616/616 of 8c0zA
- binding iron/sulfur cluster: R181 (= R180), C295 (= C287), C298 (= C290), C302 (= C294), C509 (= C493)
- binding tungsten cofactor: R75 (= R75), N92 (= N92), G94 (= G94), R181 (= R180), A183 (= A182), G184 (= G183), R185 (= R184), E329 (= E313), E331 (= E315), N356 (= N340), P362 (≠ T346), L465 (≠ M449), D504 (= D488), V510 (= V494), F511 (= F495)
1b4nA Formaldehyde ferredoxin oxidoreductase from pyrococcus furiosus, complexed with glutarate (see paper)
39% identity, 100% coverage: 2:607/609 of query aligns to 3:597/611 of 1b4nA
- binding calcium ion: G179 (≠ D179), E304 (= E310), D306 (≠ E313)
- binding glutaric acid: Y307 (= Y314), E308 (= E315), Y416 (= Y427), H437 (= H448), R473 (≠ S489), R484 (≠ N500)
- binding tungstopterin cofactor: K75 (≠ R75), G91 (≠ S91), N92 (= N92), L93 (≠ A93), G94 (= G94), R180 (= R180), A181 (= A181), A182 (= A182), G183 (= G183), R184 (= R184), D306 (≠ E313), E308 (= E315), N309 (≠ T316), D333 (≠ N340), M337 (= M344), D338 (= D345), T339 (= T346), H436 (≠ S447), H437 (= H448), K438 (≠ M449), F477 (≠ C493), E478 (≠ V494), A482 (≠ V498), C483 (≠ R499), R484 (≠ N500), L485 (= L501)
- binding iron/sulfur cluster: K75 (≠ R75), G283 (≠ A286), C284 (= C287), C287 (= C290), M289 (≠ I292), C291 (= C294), C483 (≠ R499), L485 (= L501)
1b25A Formaldehyde ferredoxin oxidoreductase from pyrococcus furiosus (see paper)
39% identity, 100% coverage: 2:607/609 of query aligns to 3:597/611 of 1b25A
- binding tungstopterin: K75 (≠ R75), G91 (≠ S91), N92 (= N92), L93 (≠ A93), G94 (= G94), G179 (≠ D179), R180 (= R180), A181 (= A181), G183 (= G183), R184 (= R184), T240 (= T242), E304 (= E310), D306 (≠ E313), Y307 (= Y314), E308 (= E315), N309 (≠ T316), D333 (≠ N340), D338 (= D345), T339 (= T346), I340 (= I347), H436 (≠ S447), H437 (= H448), K438 (≠ M449), E478 (≠ V494), A482 (≠ V498), C483 (≠ R499), R484 (≠ N500), L485 (= L501)
- binding iron/sulfur cluster: K75 (≠ R75), R180 (= R180), C284 (= C287), C287 (= C290), M289 (≠ I292), C291 (= C294), C483 (≠ R499)
6x1oC Wor5 from pyrococcus furiosus, as crystallized (see paper)
31% identity, 100% coverage: 3:609/609 of query aligns to 4:610/624 of 6x1oC
- binding magnesium ion: M194 (≠ D179), D323 (≠ E310), D326 (≠ E313)
- binding phosphate ion: R249 (= R238), Y296 (≠ A284)
- binding tungstopterin cofactor: K77 (≠ R75), S93 (= S91), V94 (≠ N92), L95 (≠ A93), S96 (≠ G94), H195 (≠ R180), A196 (= A181), A197 (= A182), G198 (= G183), Y199 (≠ R184), D326 (≠ E313), E328 (= E315), D353 (≠ N340), D358 (= D345), G359 (≠ T346), I360 (= I347), H469 (≠ P467), T470 (≠ L468), Y489 (≠ I487), D490 (= D488), I494 (≠ L492), C495 (= C493), N496 (≠ V494), F497 (= F495)
- binding iron/sulfur cluster: S96 (≠ G94), D299 (≠ C287), C302 (= C290), C306 (= C294), C495 (= C493), F497 (= F495), V498 (≠ F496)
- binding (1R)-1-hydroxybutane-1-sulfonic acid: T253 (= T242), Y327 (= Y314), E328 (= E315), H446 (≠ S447), H469 (≠ P467)
4z3yA Active site complex bambc of benzoyl coenzyme a reductase in complex with benzoyl-coa (see paper)
30% identity, 99% coverage: 5:607/609 of query aligns to 7:637/653 of 4z3yA
- binding benzoyl coenzyme A: P249 (≠ T247), W259 (≠ Y255), F323 (vs. gap), L436 (≠ G426), S439 (≠ P429), M440 (≠ R430), Y445 (≠ M435), T458 (≠ S447), E461 (≠ R450), Q466 (vs. gap), R500 (≠ K473), S504 (vs. gap)
- binding magnesium ion: M94 (≠ N92), S183 (≠ A181)
- binding iron/sulfur cluster: R77 (= R75), R181 (≠ K178), C299 (= C287), C302 (= C290), M304 (≠ I292), K305 (≠ A293), C306 (= C294), C534 (= C493)
4z3wC Active site complex bambc of benzoyl coenzyme a reductase in complex with 1,5 dienoyl-coa (see paper)
30% identity, 99% coverage: 5:607/609 of query aligns to 7:637/653 of 4z3wC
- binding 1,5 Dienoyl-CoA: P249 (≠ T247), W259 (≠ Y255), H260 (≠ P256), F323 (vs. gap), L436 (≠ G426), L438 (≠ E428), S439 (≠ P429), M440 (≠ R430), Y445 (≠ M435), T458 (≠ S447), E461 (≠ R450), Q466 (vs. gap), P499 (≠ D472), R500 (≠ K473), S504 (vs. gap)
- binding magnesium ion: M94 (≠ N92), S183 (≠ A181)
- binding iron/sulfur cluster: R77 (= R75), R181 (≠ K178), C299 (= C287), C302 (= C290), M304 (≠ I292), C306 (= C294), C534 (= C493)
4z3zA Active site complex bambc of benzoyl coenzyme a reductase in complex with zinc (see paper)
30% identity, 99% coverage: 5:607/609 of query aligns to 7:637/652 of 4z3zA
- binding magnesium ion: M94 (≠ N92), S183 (≠ A181)
- binding iron/sulfur cluster: R77 (= R75), R181 (≠ K178), C299 (= C287), C302 (= C290), M304 (≠ I292), C306 (= C294), C534 (= C493)
- binding zinc ion: E251 (≠ T249), H255 (≠ G253), E257 (vs. gap), H260 (≠ P256)
Query Sequence
>WP_073037802.1 NCBI__GCF_900129305.1:WP_073037802.1
MGWMGKILRVNLTEGATAVEELDRQKAVDYVGGRGLGTRFLLDEVDPTCDPLSPENKLIF
LTGPLTGTGTPTGARYMVMTKSPLTGALTCSNAGGQFPTMLKKAGFDGIIFEGRAEKPVY
LWVEEGRAELRSAEHLWGRDTHETDRMLREETRKEARTAVIGPAGERGVLFASIMNDKDR
AAGRSGVGAVMGSKNLKGVAVFGTQKVPIHDPEGFKKVVKEIMAGFKEQTKDGPPPLRIY
GTPITSTGTQNVGAYPTRNFQQGTFEHWQDIGGEALTEKYLVRAKACFSCPIACGRVTRV
AEGPFQGEGEGPEYETVYALGSNCGVRDLAAVIKANYICNEMGMDTITMGATIACAMEMF
EKGILDEKTVGRPLPFGDAEAMVDMVRKTAAREGFGNDLALGSLRLAAKYGCPELAMVSK
GQEFAGYEPRAEKGMGLAYATSPIGASHMRGDPAYIEIIGVPMLVDPLSWEDKPQIVKDW
QDVFALIDSAGLCVFFTVRNLVSKTKDIRPVRILELLNTATGAGYTMESLMTAAERIINA
ERIFLNKAGLDAKDDTLPPRMTREPMPTGPAKGHVCELDKMLPIYYRLREWDENGRPTPA
LLRRLGLAD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory