SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_073038629.1 NCBI__GCF_900129305.1:WP_073038629.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kctA Crystal structure of the hydrogenobacter thermophilus 2-oxoglutarate carboxylase (ogc) biotin carboxylase (bc) domain dimer in complex with adenosine 5'-diphosphate magnesium salt (mgadp), adenosine 5'- diphosphate (adp, and bicarbonate anion (hydrogen carbonate/hco3-) (see paper)
52% identity, 94% coverage: 1:453/480 of query aligns to 3:453/453 of 7kctA

query
sites
7kctA

M

M

F

F

K

K

L

V

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

C

R

R

I

V

M

I

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

Q

T

T

V

V

A

A

I

I

Y

Y

E

N

E

E

T

I

D

E

K

S

T

T

A

A

M

R

H

H

I

V

M

K

K

M

S

A

D

D

E

E

A

A

V

Y

C

M

I

I

G

G

S

V

G

N

P

P

R

L

K

D

D

T

Y

Y

L

L

D

N

I

A

D

E

R

R

I

I

I

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Q

D

A

L

A

A

K

L

A

E

V

V

G

G

A

A

D

E

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

A

E

E

N

N

P

E

L

H

F

F

S

A

K

R

R

L

C

C

T

E

D

E

N

K

G

G

L

I

I

T

F

F

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G

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P

P

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P

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K

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E

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L

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M

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G

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x
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K
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K

V

A

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x
R

A

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R

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K

E

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M

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A

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-

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A

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E

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A

-

R

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H

-

G

G

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Y

P

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M
x
L

V

L

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K

A

A

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S

A

A

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G
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G

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I
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I

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D

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T

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K

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F

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R

D

I

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Y

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L

L

E
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E

K

K

G
x
Y

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x
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E

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N

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H
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H

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I

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E

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F

Q

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I

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L

A

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D

D

G

K

H

Y

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G

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N

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V

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I

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H

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L

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G

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E

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R

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D

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K

M

L

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E

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I

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A

-

S

S

L

L

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A

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E

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R

-

E

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x
I

V

A

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D

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E

K

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G

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N

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Y

Y

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L

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E
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E

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M

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R

I

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Q

V
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E
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E

H

H

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T

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E

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M

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A

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V

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D

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M

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A

A

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E

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G

G

V

V

K

K

T

T

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I

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P

F

L

Y

L

K

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N

I

I

V

M

E

K

D

D

P

K

D

D

F

F

I

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G

N

K

F

F

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T

T

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R

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Y

I

L

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D

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Y

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A

E

E

active site: E276 (= E276), E289 (= E289), N291 (= N291), E297 (= E297), R339 (= R339)
binding adenosine-5'-diphosphate: K117 (= K115), L157 (≠ M158), K159 (= K160), G164 (= G165), G165 (= G166), G166 (= G167), I169 (= I170), E201 (= E202), Y203 (≠ G204), I204 (≠ L205), H209 (= H210), Q233 (= Q234), Q237 (= Q238), K238 (= K239), I278 (≠ L278), E289 (= E289), R293 (= R293), Q295 (= Q295), V296 (= V296), E297 (= E297), R339 (= R339)
binding bicarbonate ion: D116 (≠ S114), R119 (≠ I117)
binding magnesium ion: E276 (= E276), E289 (= E289)

A0A0H3JRU9 Pyruvate carboxylase; EC 6.4.1.1 from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see 2 papers)
47% identity, 99% coverage: 3:476/480 of query aligns to 5:481/1150 of A0A0H3JRU9

query
sites
A0A0H3JRU9

K

K

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

I

R

R

I

I

M

F

R
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R

S

A

A

A

Q

A

E

E

L

L

G

D

I

I

R

S

T

T

V

V

A

A

I

I

Y

Y

E

S

E

N

T

E

D

D

K

K

T

S

A

S

M

L

H

H

I

R

M

Y

K

K

S

A

D

D

E

E

A

S

V

Y

C

L

I

V

G

G

S

S

-

D

-

L

G

G

P

P

R

A

K

E

D

S

Y

Y

L

L

D

N

I

I

D

E

R

R

I

I

Q

D

A

V

A

A

K

K

A

Q

V

A

G

N

A

V

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

E

L

Q

F

F

S

A

K

R

R

R

C

C

T

A

D

E

N

E

G

G

L

I

I

K

F

F

V

I

G

G

P

P

P

H

P

L

Q

E

V

H

I

L

S

D

D

M

M

F

G

G

S

D

K
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K

V

V

I

K

A

A

R

R

K

T

V

T

M

A

T

I

E

K

A

A

G

D

I

L

P

P

V

V

I

I

P

P

G

G

S

T

G

-

V

-

L

-

A

-

E

D

G

G

Q

P

D

I

G

K

E

S

K

Y

E

E

-

L

A

A

L

K

E

E

F

F

A

A

R

E

L

E

H

A

G

G

F

F

P

P

I

L

M

M

V

I

K
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K

A

A

V

T

A

S

G

G

R

K

G

G

I

M

R

R

A

I

V

V

K

R

D

E

T

E

A

S

E

E

L

L

I

E

K

D

G

A

L

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L

R

A

A

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K

S

S

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E

A

A

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E

M

K

A

S

F

F

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G

D

N

D

S

R

E

I

V

Y

Y

L

I

E

E

K

R

G

Y

L

I

Q

D

N

N

P

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R

K

H
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H

V

I

E

E

V

V

Q

Q

I

V

L

I

A

G

D

D

G

E

H

H

G

G

N

N

V

I

I

V

H

H

L

L

G

F

T

E

R

R

N

D

C

C

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S

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Q

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R

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H

Q

Q

K

K

M

V

I

V

E

E

I

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A

A

P

P

A

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-

V

S

G

L

L

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A

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E

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C

A

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D

A

A

A

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A

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N

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K

Y

Y

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V

N

N

A

A

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G

T

T

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E

F

F

L

L

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V

D

-

S

S

E

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D

D

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E

Y

F

F

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E

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N

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A

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G

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-

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-

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K

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D

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Y

A

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L

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N

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T

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E

D

D

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P

K

L

N

N

G

D

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F

M

M

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P

S

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P

T

G

G

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T

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Y

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R

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S

P

S

G

G

H

F

G

G

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V

R

R

L

L

D

D

-

A

G

G

C

D

I

G

Y

F

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Q

G

G

Y

A

E

E

I

I

P

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Y

Y

D

D

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S

M

L

L

L

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V

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K

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L

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T

Y

H

G

A

F

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T

S

W

F

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K

E

Q

A

A

V

E

D

E

R
x
K

L

M

R

V

R

R

A

S

L

L

D

R

G

E

F

M

S

R

I

I

V

R

G

G

V

V

K

K

T

T

T

N

I

I

P

P

F

F

Y

L

K

I

Q

N

I

V

V

M

E

K

D

N

P

K

D

K

F

F

I

T

H

S

Q

G

N

D

F

Y

D

T

T

T

S

K

Y

F

I

I

D

E

T

E

H

T

P

P

Q

E

L

L

L

F

D

D

Y

I

R

Q

E

-

V

-

P

P

E

S

L

L

D

D

R

R

L

G

A

T

R

K

L

T

I

L

A

E

E

Y

I

I

N

G

A

N

Y

V

G

T

Y

I

N

N

P

G

Y

F

R21 (= R19) mutation to A: Complete loss of catalytic activity.
K119 (= K115) binding ATP
K161 (= K160) binding ATP
H211 (= H210) binding ATP
E278 (= E276) binding ATP
K411 (≠ R403) mutation to A: Complete loss of catalytic activity.

Sites not aligning to the query:

541:545 binding substrate
542 binding Mn(2+)
580 A→T: Complete loss of catalytic activity.
614 R→A: Complete loss of catalytic activity.
621 Y→A: Complete loss of catalytic activity.
712 binding Mn(2+)
741 binding Mn(2+)
743 binding Mn(2+)
838 Q→A: About 2.5-fold loss of catalytic activity.
876 T→A: Complete loss of catalytic activity.
879 S→A: About 2-fold loss of catalytic activity.
880 K→T: Complete loss of catalytic activity.

2vqdA Crystal structure of biotin carboxylase from pseudomonas aeruginosa complexed with ampcp (see paper)
52% identity, 92% coverage: 1:443/480 of query aligns to 1:441/447 of 2vqdA

query
sites
2vqdA

M

M

F

L

K

E

K

K

L

V

L

L

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

E

T

T

A

D

D

K

R

T

E

A

L

M

M

H

H

I

L

M

S

K

L

S

A

D

D

E

E

A

S

V

V

C

C

I

I

G

G

S

P

G

A

P

P

-

A

R

T

K

Q

D

S

Y

Y

L

L

D

Q

I

I

D

P

R

A

I

I

Q

A

A

A

K

E

A

V

V

T

G

G

A

A

D

T

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

A

E

E

N

N

P

A

L

D

F

F

S

A

K

E

R

Q

C

I

T

E

D

R

N

S

G

G

L

F

I

T

F

F

V

V

G

G

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P

P

T

P

A

Q

E

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V

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S

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D

L

M

M

G

G

S

D

K
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K

V

V

I

S

A

A

R

K

K

D

V

A

M

M

T

K

E

R

A

A

G

G

I

V

P

P

V

T

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

P

E

E

G

D

Q

-

D

-

G

-

E

E

K

E

E

T

A

A

L

L

E

A

F

I

A

A

R

R

L

E

H

V

G

G

F

Y

P

P

I

V

M
x
I

V

I

K
|
K

A

A

V

A

A

G

G

G

G
|
G

G

G

G
|
G

R

R

G

G

I

M

R

R

A

V

V

V

K

Y

D

D

T

E

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E

E

L

L

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I

K

K

G

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K

L

L

A

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R

S

T

E

E

A

A

R

G

M

A

A

A

F

F

G

G

D

N

D
x
P

R

M

I

V

Y

Y

L

L

E

E

K

K

G
x
F

L
|
L

Q

T

N

N

P

P

R

R

H
|
H

V

V

E

E

V

V

Q

Q

I

V

L

L

A

S

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

H

L

L

G

G

T

D

R

R

N

D

C

C

S

S

I

L

Q
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Q

R

R

R
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R

H
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H

Q

Q

K

K

M

V

I

I

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

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V

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E

E

-

K

-

A

-

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R

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A

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A

C

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V

R

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A

A

A

C

Q

I

A

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T

I

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G

Y

Y

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N

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A

A

G

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T
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T

V

F

E
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E

F

F

L
|
L

V

Y

D

E

S

N

E

-

D

G

Q

R

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

V

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

S

E

E

M

M

V

V

T

T

G

G

V

V

D

D

I

I

V

V

R

K

E

E

Q

M

L

L

R

R

I

I

A

A

A

S

G

G

E

E

P

K

L

L

S

S

F

I

R

R

Q

Q

E

E

D

D

V

V

Q

V

V

I

R

R

G

G

Y

H

A

A

I

L

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

K

N

T

G

-

F

F

M

M

P

P

S

S

P

P

G

G

L

K

I

V

R

K

I

H

Y

F

Q

H

S

A

P

P

G

G

H

N

G

G

V

V

R

R

L

V

D

D

G

S

C

H

I

L

Y

Y

Q

S

G

G

Y

Y

E

S

I

V

P

P

R

P

Y

N

Y

Y

D

D

S

S

M

L

L

V

V

G

K

K

L

V

T

I

V

T

Y

Y

G

G

F

A

T

D

W

R

R

D

E

E

A

A

V

L

D

A

R

R

L

M

R

R

R

N

A

A

L

L

D

D

G

E

F

L

S

I

I

V

V

D

G

G

V

I

K

K

T

T

T

N

I

T

P

E

F

L

Y

H

K

K

Q

D

I

L

V

V

E

R

D

D

P

A

D

A

F

F

I

C

H

K

Q

G

N

G

F

V

D

N

T
x
I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), P196 (≠ D197), H209 (= H210), R235 (= R236), T274 (= T274), E276 (= E276), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
binding phosphomethylphosphonic acid adenosyl ester: K116 (= K115), I157 (≠ M158), K159 (= K160), G164 (= G165), G166 (= G167), F203 (≠ G204), L204 (= L205), H209 (= H210), Q233 (= Q234), H236 (= H237), L278 (= L278), E288 (= E289), I437 (≠ T439)
binding magnesium ion: E276 (= E276), E288 (= E289)

2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:439/444 of 2vr1A

query
sites
2vr1A

M

M

F

L

K

D

K

K

L

I

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

V

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

S

A

A

K

E

A

I

V

T

G

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

N

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

I

F

F

V

I

G

G

P

P

P

K

P

A

Q

E

V

T

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

A

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

G

E

D

G

D

Q

M

D

D

G

K

E

N

K

R

E

A

A

-

L

-

E

-

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

V

M

I

V

I

K
|
K

A

A

V

-

A

-

G

S

G

G

R
|
R

G

G

I
x
M

R

R

A

V

V

V

K

R

D

G

T

D

A

A

E

E

L

L

I

A

K

Q

G

S

L

I

R

S

L

M

A

T

R

R

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

S

D

N

D
|
D

R

M

I

V

Y

Y

L

M

E

E

K

K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

R
|
R

H

H

Q

Q

K

K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

P

E

E

-

L

-

R

R

Y

I

I

C

G

A

E

D

R

A

C

V

A

R

K

A

A

A

C

Q

V

A

D

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

F

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

I

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

Q

P

P

L

L

S

S

F

I

R

K

Q

Q

E

E

D

E

V

V

Q

H

V

V

R

R

G

G

Y

H

A

A

I

V

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

-

N

N

G

T

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

I

R

T

I

R

Y

F

Q

H

S

A

P

P

G

G

H

F

G

G

V

V

R

R

L

W

D

E

G

S

C

H

I

I

Y

Y

Q

A

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

Y

D

D

S

S

M

M

L

I

V

G

K

K

L

L

T

I

V

C

Y

Y

G

G

F

E

T

N

W

R

R

D

E

V

A

A

V

I

D

A

R

R

L

M

R

K

R

N

A

A

L

L

D

Q

G

E

F

L

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

V

P

D

F

L

Y

Q

K

I

Q

R

I

I

V

M

E

N

D

D

P

E

D

N

F

F

I

Q

H

H

Q

G

N

G

F

T

D

N

T
x
I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), D194 (= D197), H207 (= H210), R233 (= R236), T272 (= T274), E274 (= E276), E286 (= E289), N288 (= N291), R290 (= R293), E294 (= E297), R336 (= R339)
binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K160), R165 (= R168), M167 (≠ I170), Y201 (≠ G204), L202 (= L205), E274 (= E276), L276 (= L278), E286 (= E289), N288 (= N291), I435 (≠ T439)

4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:438/442 of 4mv4A

query
sites
4mv4A

M

M

F

L

K

E

K

K

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

E

T

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

A

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

A

A

A

K

E

A

V

V

T

G

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

D

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

T

F

F

V

I

G

G

P

P

P

T

P

A

Q

D

V

V

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

K

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

G

D

V

G

L

P

A

V

E

S

G

N

Q

D

D

I

G

A

E

K

K

N

E

K

A

-

L

-

E

E

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

I

M

I

V

I

K
|
K

A

A

V

S

A

G

G

G

G
|
G

G

G

G

-

R

-

G

-

I
x
M

R

R

A

V

V

V

K

R

D

S

T

E

A

D

E

A

L

L

I

E

K

E

G

S

L

I

R

A

L

M

A

T

R

K

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

N

D

N

D
|
D

R

M

I

V

Y

Y

L

M

E
|
E

K

K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

T

H

H

G

G

N

N

V

A

I

V

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

R
|
R

H
|
H

Q

Q

K

K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

E

-

V

-

R

R

D

I

I

C

G

A

S

D

R

A

C

V

A

R

N

A

A

A

C

Q

V

A

E

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

Y

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

V

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

L

P

P

L

I

S

S

F

F

R

K

Q

Q

E

E

D

D

V

I

Q

K

V

V

R

K

G

G

Y

H

A

A

I

M

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

K

N

T

G

-

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

V

R

N

I

H

Y

L

Q

H

S

S

P

P

G

G

H

L

G

G

V

V

R

R

L

W

D

D

G

S

C

H

I

V

Y

Y

Q

G

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

H

Y

Y

D

D

S

S

M

M

L

I

V

A

K

K

L

L

T

I

V

T

Y

Y

G

G

F

D

T

T

W

R

R

E

E

V

A

A

V

I

D

R

R

R

L

M

R

Q

R

N

A

A

L

L

D

S

G

E

F

T

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

I

P

P

F

L

Y

H

K

E

Q

L

I

I

V

L

E

E

D

D

P

E

D

N

F

F

I

Q

H

K

Q

G

N

G

F

T

D

N

T

I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), D193 (= D197), H206 (= H210), R232 (= R236), T271 (= T274), E273 (= E276), E285 (= E289), N287 (= N291), R289 (= R293), E293 (= E297), R335 (= R339)
binding phosphomethylphosphonic acid adenylate ester: K159 (= K160), G164 (= G165), M166 (≠ I170), E198 (= E202), Y200 (≠ G204), L201 (= L205), H233 (= H237), L275 (= L278), E285 (= E289)
binding magnesium ion: E273 (= E276), E285 (= E289)

3bg5A Crystal structure of staphylococcus aureus pyruvate carboxylase (see paper)
46% identity, 99% coverage: 3:476/480 of query aligns to 3:472/1137 of 3bg5A

query
sites
3bg5A

K

K

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

I

R

R

I

I

M

F

R

R

S

A

A

A

Q

A

E

E

L

L

G

D

I

I

R

S

T

T

V

V

A

A

I

I

Y

Y

E

S

E

N

T

E

D

D

K

K

T

S

A

S

M

L

H

H

I

R

M

Y

K

K

S

A

D

D

E

E

A

S

V

Y

C

L

I

V

G

G

S

S

-

D

-

L

G

G

P

P

R

A

K

E

D

S

Y

Y

L

L

D

N

I

I

D

E

R

R

I

I

Q

D

A

V

A

A

K

K

A

Q

V

A

G

N

A

V

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

E

L

Q

F

F

S

A

K

R

R

R

C

C

T

A

D

E

N

E

G

G

L

I

I

K

F

F

V

I

G

G

P

P

P

H

P

L

Q

E

V

H

I

L

S

D

D

M

M

F

G

G

S

D

K
|
K

V

V

I

K

A

A

R

R

K

T

V

T

M

A

T

I

E

K

A

A

G

D

I

L

P

P

V

V

I

I

P

P

G

G

S

T

G

-

V

-

L

-

A

-

E

D

G

G

Q

P

D

I

G

K

E

S

K

Y

E

E

-

L

A

A

L

K

E

E

F

F

A

A

R

E

L

E

H

A

G

G

F

F

P

P

I

L

M
|
M

V

I

K
|
K

A

A

V

T

A

S

G

-

R

-

G

-

I

-

R

R

A

I

V

V

K

R

D

E

T

E

A

S

E

E

L

L

I

E

K

D

G

A

L

F

R

H

L

R

A

A

R

K

S

S

E

E

A

A

R

E

M

K

A

S

F

F

G

G

D

N

D
x
S

R

E

I

V

Y

Y

L

I

E

E

K

R

G
x
Y

L
x
I

Q

D

N

N

P

P

R

K

H
|
H

V

I

E

E

V

V

Q

Q

I

V

L

I

A

G

D

D

G

E

H

H

G

G

N

N

V

I

I

V

H

H

L

L

G

F

T

E

R

R

N

D

C

C

S

S

I

V

Q
|
Q

R

R

R
|
R

H
|
H

Q

Q

K

K

M

V

I

V

E

E

I

V

A

A

P

P

A

S

-

V

S

G

L

L

P

S

A

P

L

T

V

L

E

R

E

Q

R

R

I

I

C

C

A

D

D

A

A

A

V

I

R

Q

A

L

A

M

Q

E

A

N

T

I

D

K

Y

Y

V

V

N

N

A

A

G

G

T
|
T

V

V

E
|
E

F

F

L
|
L

V

V

D

-

S

S

E

G

D

D

Q

E

Y

F

F

F

L

I

E
|
E

V

V

N
|
N

T

P

R
|
R

I

V

Q

Q

V

V

E
|
E

H

H

T

T

V

I

T

T

E

E

M

M

V

V

T

T

G

G

V

I

D

D

I

I

V

V

R

K

E

T

Q

Q

L

I

R

L

I

V

A

A

G

G

E

A

P

D

L

L

-

F

-

G

-

E

-

I

-

N

S

M

F

P

R

Q

Q

Q

E

K

D

D

V

I

Q

T

V

T

R

L

G

G

Y

Y

A

A

I

I

E

Q

L

C

R
|
R

I

I

N

T

A

T

E

E

D

D

P

P

K

L

N

N

G

D

F

F

M

M

P

P

S

D

P

T

G

G

L

T

I

I

R

I

I

A

Y

Y

Q

R

S

S

P

S

G

G

H

F

G

G

V

V

R

R

L

L

D

D

-

A

G

G

C

D

I

G

Y

F

Q

Q

G

G

Y

A

E

E

I

I

P

S

R

P

Y

Y

D

D

S

S

M

L

L

L

V

V

K

K

L

L

T

S

V

T

Y

H

G

A

F

I

T

S

W

F

R

K

E

Q

A

A

V

E

D

E

R

K

L

M

R

V

R

R

A

S

L

L

D

R

G

E

F

M

S

R

I

I

V

R

G

G

V

V

K

K

T

T

T

N

I

I

P

P

F

F

Y

L

K

I

Q

N

I

V

V

M

E

K

D

N

P

K

D

K

F

F

I

T

H

S

Q

G

N

D

F

Y

D

T

T

T

S

K

Y

F

I

I

D

E

T

E

H

T

P

P

Q

E

L

L

L

F

D

D

Y

I

R

Q

E

-

V

-

P

P

E

S

L

L

D

D

R

R

L

G

A

T

R

K

L

T

I

L

A

E

E
x
Y

I

I

N

G

A

N

Y

V

G

T

Y

I

N

N

P
x
G

Y
x
F

active site: K117 (= K115), K159 (= K160), S189 (≠ D197), H202 (= H210), R228 (= R236), T267 (= T274), E269 (= E276), E281 (= E289), N283 (= N291), R285 (= R293), E289 (= E297), R337 (= R339)
binding adenosine-5'-triphosphate: K117 (= K115), M157 (= M158), K159 (= K160), Y196 (≠ G204), I197 (≠ L205), H202 (= H210), Q226 (= Q234), H229 (= H237), E269 (= E276), L271 (= L278), E281 (= E289), N283 (= N291)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: Y463 (≠ E467), G471 (≠ P475), F472 (≠ Y476)

Sites not aligning to the query:

active site: 533, 639, 703, 732, 734, 755, 761, 762, 801, 867, 869, 881, 883, 888
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: 473, 579
binding manganese (ii) ion: 533, 732, 734
binding pyruvic acid: 603, 703

2vpqB Crystal structure of biotin carboxylase from s. Aureus complexed with amppnp (see paper)
49% identity, 93% coverage: 3:450/480 of query aligns to 1:448/448 of 2vpqB

query
sites
2vpqB

K

K

L

V

L

L

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

V

R

R

I

I

M

I

R

R

S

A

A

C

Q

R

E

D

L

L

G

G

I

I

R

Q

T

T

V

V

A

A

I

I

Y

Y

E

S

E

E

T

G

D

D

K

K

T

D

A

A

M

L

H

H

I

T

M

Q

K

I

S

A

D

D

E

E

A

A

V

Y

C

C

I

V

G

G

S

P

G

T

P

L

R

S

K

K

D

D

-

S

Y

Y

L

L

D

N

I

I

D

P

R

N

I

I

I

L

Q

S

A

I

A

A

K

T

A

S

V

T

G

G

A

C

D

D

A

G

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

A

E

E

N

N

P

A

L

D

F

F

S

A

K

E

R

L

C

C

T

E

D

A

N

C

G

Q

L

L

I

K

F

F

V

I

G

G

P

P

P

S

P

Y

Q

Q

V

S

I

I

S

Q

D

K

M

M

G

G

S

I

K
|
K

V

D

I
x
V

A

A

R

K

K

A

V

E

M

M

T

I

E

K

A

A

G

N

I

V

P

P

V

V

I

V

P

P

G

G

S

S

G

D

V

G

L

L

A

M

E

K

G

D

Q

V

D

-

G

-

E

-

K

S

E

E

A

A

L

K

E

K

F

I

A

A

R

K

L

K

H

I

G

G

F

Y

P

P

I

V

M
x
I

V

I

K
|
K

A

A

V

T

A

A

G

G

G
|
G

G

G

G
|
G

R

K

G

G

I
|
I

R

R

A

V

V

A

K

R

D

D

T

E

A

K

E

E

L

L

I

E

K

T

G

G

L

F

R

R

L

M

A

T

R

E

S

Q

E

E

A

A

R

Q

M

T

A

A

F

F

G

G

D

N

D

G

R

G

I

L

Y

Y

L

M

E

E

K

K

G
x
F

L
x
I

Q

E

N

N

P

F

R

R

H
|
H

V

I

E

E

V

I

Q

Q

I

I

L

V

A

G

D

D

G

S

H

Y

G

G

N

N

V

V

I

I

H

H

L

L

G

G

T

E

R

R

N

D

C

C

S

T

I

I

Q

Q

R

R

R
|
R

H

M

Q

Q

K

K

M

L

I

V

E

E

I

E

A

A

P

P

A

S

S

-

L

-

P

P

A

I

L

L

V

D

E

D

E

E

-

T

-

R

R

E

I

M

C

G

A

N

D

A

A

A

V

V

R

R

A

A

Q

K

A

A

T

V

D

N

Y

Y

V

E

N

N

A

A

G

G

T
|
T

V

I

E
|
E

F

F

L
x
I

V

Y

D

D

-

L

S

N

E

D

D

N

Q

K

Y

F

Y

Y

F

F

L
x
M

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

V

T

T

G

G

V

I

D

D

I

L

V

V

R

K

E

L

Q

Q

L

L

R

Q

I

V

A

A

A

M

G

G

E

D

P

V

L

L

S

P

F

Y

R

K

Q

Q

E

E

D

D

V

I

Q

K

V

L

R

T

G

G

Y

H

A

A

I

I

E

E

L

F

R
|
R

I

I

N

N

A

A

E

E

D

N

P

P

K

Y

N

K

G

N

F

F

M

M

P

P

S

S

P

P

G

G

L

K

I

I

R

E

I

Q

Y

Y

Q

L

S

A

P

P

G

G

H

Y

G

G

V

V

R

R

L

I

D

E

G

S

C

A

I

C

Y

Y

Q

T

G

N

Y

Y

E

T

I

I

P

P

R

P

Y

Y

D

D

S

S

M

M

L

V

V

A

K

K

L

L

T

I

V

I

Y

H

G

E

F

P

T

T

W

R

R

D

E

E

A

A

V

I

D

M

R

A

L

G

R

I

R

R

A

A

L

L

D

S

G

E

F

F

S

V

I

V

V

L

G

G

V

I

K

D

T

T

I

I

P

P

F

F

Y

H

K

I

Q

K

I

L

V

L

E

N

D

N

P

D

D

I

F

F

I

R

H

S

Q

G

N

K

F

F

D

N

T

T

S

N

Y

F

I

L

D

E

T

Q

H

N

P

S

Q

I

L

M

L

N

D

D

active site: V116 (≠ I117), K156 (= K160), H206 (= H210), R232 (= R236), T271 (= T274), E273 (= E276), E287 (= E289), N289 (= N291), R291 (= R293), E295 (= E297), R337 (= R339)
binding phosphoaminophosphonic acid-adenylate ester: K114 (= K115), I154 (≠ M158), K156 (= K160), G161 (= G165), G163 (= G167), I166 (= I170), F200 (≠ G204), I201 (≠ L205), E273 (= E276), I275 (≠ L278), M286 (≠ L288), E287 (= E289)
binding magnesium ion: E273 (= E276), E287 (= E289)

4mv3A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and bicarbonate (see paper)
48% identity, 92% coverage: 1:443/480 of query aligns to 1:435/439 of 4mv3A

query
sites
4mv3A

M

M

F

L

K

E

K

K

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

E

T

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

A

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

A

A

A

K

E

A

V

V

T

G

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

D

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

T

F

F

V

I

G

G

P

P

P

T

P

A

Q

D

V

V

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

K

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

G

D

V

G

L

P

A

V

E

S

G

N

Q

D

D

I

G

A

E

K

K

N

E

K

A

-

L

-

E

E

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

I

M

I

V

I

K
|
K

A

A

V

-

A

-

G

-

R

S

G

G

I
x
M

R

R

A

V

V

V

K

R

D

S

T

E

A

D

E

A

L

L

I

E

K

E

G

S

L

I

R

A

L

M

A

T

R

K

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

N

D

N

D
|
D

R

M

I

V

Y

Y

L

M

E
|
E

K

K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

T

H

H

G

G

N

N

V

A

I

V

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

R
|
R

H

H

Q

Q

K

K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

E

-

V

-

R

R

D

I

I

C

G

A

S

D

R

A

C

V

A

R

N

A

A

A

C

Q

V

A

E

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

Y

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q
|
Q

V
|
V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

V

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

L

P

P

L

I

S

S

F

F

R

K

Q

Q

E

E

D

D

V

I

Q

K

V

V

R

K

G

G

Y

H

A

A

I

M

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

K

N

T

G

-

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

V

R

N

I

H

Y

L

Q

H

S

S

P

P

G

G

H

L

G

G

V

V

R

R

L

W

D

D

G

S

C

H

I

V

Y

Y

Q

G

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

H

Y

Y

D

D

S

S

M

M

L

I

V

A

K

K

L

L

T

I

V

T

Y

Y

G

G

F

D

T

T

W

R

R

E

E

V

A

A

V

I

D

R

R

R

L

M

R

Q

R

N

A

A

L

L

D

S

G

E

F

T

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

I

P

P

F

L

Y

H

K

E

Q

L

I

I

V

L

E

E

D

D

P

E

D

N

F

F

I

Q

H

K

Q

G

N

G

F

T

D

N

T

I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), D190 (= D197), H203 (= H210), R229 (= R236), T268 (= T274), E270 (= E276), E282 (= E289), N284 (= N291), R286 (= R293), E290 (= E297), R332 (= R339)
binding phosphomethylphosphonic acid adenylate ester: K159 (= K160), M163 (≠ I170), E195 (= E202), Y197 (≠ G204), L198 (= L205), E270 (= E276), L272 (= L278), E282 (= E289)
binding bicarbonate ion: R286 (= R293), Q288 (= Q295), V289 (= V296)

6oi8A Crystal structure of haemophilus influenzae biotin carboxylase complexed with 7-((1r,5s,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl)- 6-(2-chloro-6-(pyridin-3-yl)phenyl)pyrido[2,3-d]pyrimidin-2-amine (see paper)
48% identity, 92% coverage: 1:443/480 of query aligns to 1:436/440 of 6oi8A

query
sites
6oi8A

M

M

F

L

K

E

K

K

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

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R

I

I

M

L

R

R

S

A

A

C

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K

E

E

L

L

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G

I

I

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K

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T

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V

A

A

I

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Y

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E

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E

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D

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D

A

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A

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E

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C

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G

A

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-

A

K

K

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S

Y

Y

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I

Q

A

A

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A

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A

A

I

I

H

H

P

P

G

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Y

Y

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G

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N

P

A

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F

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K

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x
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H

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A

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G

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I

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K

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T

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N

I

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x
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E

active site: K116 (= K115), K159 (= K160), D191 (= D197), H204 (= H210), R230 (= R236), T269 (= T274), E271 (= E276), E283 (= E289), N285 (= N291), R287 (= R293), E291 (= E297), R333 (= R339)
binding 7-[(1R,5S,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl]-6-[2-chloro-6-(pyridin-3-yl)phenyl]pyrido[2,3-d]pyrimidin-2-amine: I157 (≠ M158), K159 (= K160), M164 (≠ I170), E196 (= E202), Y198 (≠ G204), L199 (= L205), H204 (= H210), Q228 (= Q234), E271 (= E276), L273 (= L278), E283 (= E289), I432 (≠ T439)

7zz3A Cryo-em structure of "bc react" conformation of lactococcus lactis pyruvate carboxylase with acetyl-coa (see paper)
49% identity, 94% coverage: 2:451/480 of query aligns to 2:455/1138 of 7zz3A

query
sites
7zz3A

F

M

K

K

L

L

V

V

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A

N

N

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G

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E

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I

A

A

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N

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L

L

E

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F

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A

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A

A

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-

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-

A

A

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|
G

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x
M

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D

N

T

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A

A

E

E

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H

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Q

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E

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R

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F

binding acetyl coenzyme *a: N22 (≠ Q22), F43 (≠ M43), K44 (= K44), A45 (≠ S45), D46 (= D46), S48 (≠ A48), R363 (≠ Q360), H413 (≠ D409), E414 (≠ G410), R416 (≠ S412), R418 (≠ V414)
binding adenosine-5'-triphosphate: K117 (= K115), M156 (= M158), K158 (= K160), G163 (= G165), G164 (= G166), G165 (= G167), M168 (≠ I170), E200 (= E202), Y202 (≠ G204), I203 (≠ L205), H208 (= H210), Q232 (= Q234), N235 (≠ H237), L277 (= L278), E287 (= E289), N289 (= N291), T443 (= T439)
binding bicarbonate ion: K237 (= K239), R291 (= R293), Q293 (= Q295), E295 (= E297)
binding biotin: G84 (= G82), V294 (= V296), R342 (= R339)
binding magnesium ion: E275 (= E276), E287 (= E289)

Sites not aligning to the query:

binding acetyl coenzyme *a: 459, 461, 1016, 1017, 1018, 1045
binding biotin: 1104
binding magnesium ion: 520, 523, 754
binding manganese (ii) ion: 535, 704, 733, 735
binding pyruvic acid: 534, 538, 605, 704, 868

6ojhA Crystal structure of haemophilus influenzae biotin carboxylase complexed with (r)-7-(3-aminopyrrolidin-1-yl)-6-(naphthalen-1-yl) pyrido[2,3-d]pyrimidin-2-amine
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/445 of 6ojhA

query
sites
6ojhA

M

M

F

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E

K

K

L

V

V

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A

N

N

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A

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F

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N

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E

F

F

L
|
L

V

Y

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

V

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

L

P

P

L

I

S

S

F

F

R

K

Q

Q

E

E

D

D

V

I

Q

K

V

V

R

K

G

G

Y

H

A

A

I

M

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

K

N

T

G

-

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

V

R

N

I

H

Y

L

Q

H

S

S

P

P

G

G

H

L

G

G

V

V

R

R

L

W

D

D

G

S

C

H

I

V

Y

Y

Q

G

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

H

Y

Y

D

D

S

S

M

M

L

I

V

A

K

K

L

L

T

I

V

T

Y

Y

G

G

F

D

T

T

W

R

R

E

E

V

A

A

V

I

D

R

R

R

L

M

R

Q

R

N

A

A

L

L

D

S

G

E

F

T

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

I

P

P

F

L

Y

H

K

E

Q

L

I

I

V

L

E

E

D

D

P

E

D

N

F

F

I

Q

H

K

Q

G

N

G

F

T

D

N

T
x
I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), D196 (= D197), H209 (= H210), R235 (= R236), T274 (= T274), E276 (= E276), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
binding calcium ion: E276 (= E276), E288 (= E289), N290 (= N291)
binding 7-[(3R)-3-aminopyrrolidin-1-yl]-6-(naphthalen-1-yl)pyrido[2,3-d]pyrimidin-2-amine: K159 (= K160), M169 (≠ I170), E201 (= E202), Y203 (≠ G204), L204 (= L205), H236 (= H237), L278 (= L278), E288 (= E289), I437 (≠ T439)

5vyzA Crystal structure of lactococcus lactis pyruvate carboxylase in complex with cyclic-di-amp (see paper)
49% identity, 94% coverage: 2:451/480 of query aligns to 8:461/1144 of 5vyzA

query
sites
5vyzA

F

M

K

K

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

V

R

R

I

V

M

F

R

R

S

A

A

C

Q

N

E

E

L

L

G

G

I

L

R

S

T

T

V

V

A

A

I

V

Y

Y

E

A

E

R

T

E

D

D

K

E

T

Y

A

S

M

V

H

H

I

R

M

F

K

K

S

A

D

D

E

E

A

S

V

Y

C

L

I

I

G

G

S

Q

G

G

-

K

P

P

R

I

K

D

D

A

Y

Y

L

L

D

D

I

I

D

D

R

D

I

I

Q

R

A

V

A

A

K

L

A

E

V

S

G

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

L

L

L

A

S

E

E

N

N

P

L

L

E

F

F

S

A

K

T

R

K

C

V

T

R

D

A

N

A

G

G

L

L

I

V

F

F

V

V

G

G

P

P

P

E

P

L

Q

H

V

H

I

L

S

D

D

I

M

F

G

G

S

D

K
|
K

V

I

I

K

A

A

R

K

K

A

V

A

M

A

T

D

E

E

A

A

G

K

I

V

P

P

V

G

I

I

P

P

G

G

S

T

G

N

V

G

L

-

A

A

E

V

G

D

Q

I

D

D

G

G

E

-

K

-

E

-

A

A

L

L

E

E

F

F

A

A

R

K

L

T

H

Y

G

G

F

Y

P

P

I

V

M
|
M

V

I

K
|
K

A

A

V

A

A

L

G
|
G

G

G

G
|
G

R

R

G

G

I
x
M

R

R

A

V

V

A

K

R

D

N

T

D

A

A

E

E

L

M

I

H

K

D

G

G

L

Y

R

A

L

R

A

A

R

K

S

S

E

E

A

A

R

I

M

G

A

A

F

F

G

G

D

S

D

G

R

E

I

I

Y

Y

L

V

E

E

K

K

G
x
Y

L
x
I

Q

E

N

N

P

P

R

K

H
|
H

V

I

E

E

V

V

Q

Q

I

I

L

L

A

G

D

D

G

R

H

H

G

G

N

N

V

I

I

I

H

H

L

L

G

H

T

E

R

R

N

D

C

C

S

S

I

V

Q
|
Q

R

R

H
x
N

Q

Q

K

K

M

V

I

I

E

E

I

I

A

A

P

P

A

A

-

V

S

G

L

L

P

S

A

P

L

D

V

F

E

R

E

N

R

E

I

I

C

C

A

E

D

A

A

A

V

V

R

K

A

L

A

C

Q

K

A

N

T

V

D

G

Y

Y

V

V

N

N

A

A

G

G

T

T

V

V

E
|
E

F

F

L
|
L

V

V

D

-

S

K

E

D

D

D

Q

K

Y

F

Y

Y

F

F

L

I

E
|
E

V

V

N

N

T

P

R

R

I

V

Q

Q

V

V

E

E

H

H

T

T

V

I

T

T

E

E

M

L

V

I

T

T

G

G

V

V

D

D

I

I

V

V

R

Q

E

A

Q

Q

L

I

R

L

I

I

A

A

A

Q

G

G

E

K

P

D

L

L

S

H

F

R

R

E

-

I

-

G

-

L

-

P

-

A

Q

Q

E

S

D

E

V

I

Q

P

V

L

R

L

G

G

Y

S

A

A

I

I

E

Q

L

C

R

R

I

I

N

T

A

T

E

E

D

D

P

P

K

Q

N

N

G

G

F

F

M

L

P

P

S

D

P

T

G

G

L

K

I

I

R

D

I

T

Y

Y

Q

R

S

S

P

P

G

G

H

F

G

G

V

I

R

R

L

L

D

D

-

V

G

G

C

N

I

A

Y

Y

Q

A

G

G

Y

Y

E

E

I

V

P

T

R

P

Y

Y

Y

F

D

D

S

S

M

L

L

L

V

V

K

K

L

V

T

C

V

T

Y

F

G

A

F

N

T

E

W

F

R

S

E

D

A

S

V

V

D

R

R

K

L

M

R

D

R

R

A

V

L

L

D

H

G

E

F

F

S

R

I

I

V

R

G

G

V

V

K

K

T

T

T

N

I

I

P

P

F

F

Y

L

K

I

Q

N

I

V

V

I

E

A

D

N

P

E

D

N

F

F

I

T

H

S

Q

G

N

Q

F

A

D

T

T
|
T

S

T

Y

F

I

I

D

D

T

N

H

T

P

P

Q

S

L

L

L

F

D

N

Y

F

binding adenosine-5'-diphosphate: K123 (= K115), M162 (= M158), K164 (= K160), G168 (= G164), G170 (= G166), G171 (= G167), M174 (≠ I170), Y208 (≠ G204), I209 (≠ L205), H214 (= H210), Q238 (= Q234), N241 (≠ H237), L283 (= L278), E293 (= E289), T449 (= T439)
binding magnesium ion: E281 (= E276), E293 (= E289)

Sites not aligning to the query:

P43873 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/448 of P43873

query
sites
P43873

M

M

F

L

K

E

K

K

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

E

T

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

A

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

A

A

A

K

E

A

V

V

T

G

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

D

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

T

F

F

V

I

G

G

P

P

P

T

P

A

Q

D

V

V

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

K

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

G

D

V

G

L

P

A

V

E

S

G

N

Q

D

D

I

G

A

E

K

K

N

E

K

A

-

L

-

E

E

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

I

M

I

V

I

K
|
K

A

A

V

S

A

G

G

G

R

R

G

G

I

M

R

R

A

V

V

V

K

R

D

S

T

E

A

D

E

A

L

L

I

E

K

E

G

S

L

I

R

A

L

M

A

T

R

K

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

N

D

N

D

D

R

M

I

V

Y

Y

L

M

E
|
E

K
|
K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H

H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

T

H

H

G

G

N

N

V

A

I

V

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

H

H

Q

Q

K

K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

E

-

V

-

R

R

D

I

I

C

G

A

S

D

R

A

C

V

A

R

N

A

A

A

C

Q

V

A

E

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T

T

V

F

E
|
E

F

F

L

L

V

Y

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R

R

I

I

Q

Q

V

V

E

E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

V

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

L

P

P

L

I

S

S

F

F

R

K

Q

Q

E

E

D

D

V

I

Q

K

V

V

R

K

G

G

Y

H

A

A

I

M

E

E

L

C

R

R

I

I

N

N

A

A

E

E

D

D

P

P

K

K

N

T

G

-

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

V

R

N

I

H

Y

L

Q

H

S

S

P

P

G

G

H

L

G

G

V

V

R

R

L

W

D

D

G

S

C

H

I

V

Y

Y

Q

G

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

H

Y

Y

D

D

S

S

M

M

L

I

V

A

K

K

L

L

T

I

V

T

Y

Y

G

G

F

D

T

T

W

R

R

E

E

V

A

A

V

I

D

R

R

R

L

M

R

Q

R

N

A

A

L

L

D

S

G

E

F

T

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

I

P

P

F

L

Y

H

K

E

Q

L

I

I

V

L

E

E

D

D

P

E

D

N

F

F

I

Q

H

K

Q

G

N

G

F

T

D

N

T

I

S

H

Y

Y

I

L

D

E

K116 (= K115) binding ATP
K159 (= K160) binding ATP
EKYL 201:204 (≠ EKGL 202:205) binding ATP
E276 (= E276) binding ATP; binding Mg(2+)
E288 (= E289) binding ATP; binding Mg(2+)
N290 (= N291) binding Mg(2+)

7zyyA Cryo-em structure of lactococcus lactis pyruvate carboxylase with acetyl-coa (see paper)
49% identity, 94% coverage: 2:451/480 of query aligns to 2:455/1056 of 7zyyA

query
sites
7zyyA

F

M

K

K

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

V

R

R

I

V

M

F

R
|
R

S

A

A

C

Q
x
N

E

E

L

L

G

G

I

L

R

S

T

T

V

V

A

A

I

V

Y

Y

E

A

E

R

T

E

D

D

K

E

T

Y

A

S

M

V

H

H

I

R

M
x
F

K
|
K

S
x
A

D

D

E

E

A

S

V

Y

C

L

I

I

G

G

S

Q

G

G

-

K

P

P

R

I

K

D

D

A

Y

Y

L

L

D

D

I

I

D

D

R

D

I

I

Q

R

A

V

A

A

K

L

A

E

V

S

G

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

L

L

L

A

S

E

E

N

N

P

L

L

E

F

F

S

A

K

T

R

K

C

V

T

R

D

A

N

A

G

G

L

L

I

V

F

F

V

V

G

G

P

P

P

E

P

L

Q

H

V

H

I

L

S

D

D

I

M

F

G

G

S

D

K

K

V

I

I

K

A

A

R

K

K

A

V

A

M

A

T

D

E

E

A

A

G

K

I

V

P

P

V

G

I

I

P

P

G

G

S

T

G

N

V

G

L

-

A

A

E

V

G

D

Q

I

D

D

G

G

E

-

K

-

E

-

A

A

L

L

E

E

F

F

A

A

R

K

L

T

H

Y

G

G

F

Y

P

P

I

V

M

M

V

I

K
|
K

A

A

V

A

A

L

G

G

G
|
G

G

G

R

R

G

G

I
x
M

R

R

A

V

V

A

K

R

D

N

T

D

A

A

E

E

L

M

I

H

K

D

G

G

L

Y

R

A

L

R

A

A

R

K

S

S

E

E

A

A

R

I

M

G

A

A

F

F

G

G

D

S

D

G

R

E

I

I

Y

Y

L

V

E
|
E

K
|
K

G
x
Y

L
x
I

Q

E

N

N

P

P

R

K

H
|
H

V

I

E

E

V

V

Q

Q

I

I

L

L

A

G

D

D

G

R

H

H

G

G

N

N

V

I

I

I

H

H

L

L

G

H

T

E

R

R

N

D

C

C

S

S

I

V

Q
|
Q

R

R

H
x
N

Q

Q

K

K

M

V

I

I

E

E

I

I

A

A

P

P

A

A

-

V

S

G

L

L

P

S

A

P

L

D

V

F

E

R

E

N

R

E

I

I

C

C

A

E

D

A

A

A

V

V

R

K

A

L

A

C

Q

K

A

N

T

V

D

G

Y

Y

V

V

N

N

A

A

G

G

T

T

V

V

E
|
E

F

F

L
|
L

V

V

D

-

S

K

E

D

D

D

Q

K

Y

F

Y

Y

F

F

L

I

E
|
E

V

V

N

N

T

P

R
|
R

I

V

Q
|
Q

V
|
V

E
|
E

H

H

T

T

V

I

T

T

E

E

M

L

V

I

T

T

G

G

V

V

D

D

I

I

V

V

R

Q

E

A

Q

Q

L

I

R

L

I

I

A

A

A

Q

G

G

E

K

P

D

L

L

S

H

F

R

R

E

-

I

-

G

-

L

-

P

-

A

Q

Q

E

S

D

E

V

I

Q

P

V

L

R

L

G

G

Y

S

A

A

I

I

E

Q

L

C

R

R

I

I

N

T

A

T

E

E

D

D

P

P

K

Q

N

N

G

G

F

F

M

L

P

P

S

D

P

T

G

G

L

K

I

I

R

D

I

T

Y

Y

Q
x
R

S

S

P

P

G

G

H

F

G

G

V

I

R

R

L

L

D

D

-

V

G

G

C

N

I

A

Y

Y

Q

A

G

G

Y

Y

E

E

I

V

P

T

R

P

Y

Y

Y

F

D

D

S

S

M

L

L

L

V

V

K

K

L

V

T

C

V

T

Y

F

G

A

F

N

T

E

W

F

R

S

E

D

A

S

V

V

D

R

R

K

L

M

R

D

R

R

A

V

L

L

D

H

G
x
E

F

F

S
x
R

I

I

V
x
R

G

G

V

V

K

K

T

T

T

N

I

I

P

P

F

F

Y

L

K

I

Q

N

I

V

V

I

E

A

D

N

P

E

D

N

F

F

I

T

H

S

Q

G

N

Q

F

A

D

T

T
|
T

S

T

Y

F

I

I

D

D

T

N

H

T

P

P

Q

S

L

L

L

F

D

N

Y

F

binding acetyl coenzyme *a: R19 (= R19), N22 (≠ Q22), F43 (≠ M43), K44 (= K44), A45 (≠ S45), R363 (≠ Q360), E414 (≠ G410), R416 (≠ S412), R418 (≠ V414)
binding adenosine-5'-diphosphate: K158 (= K160), G163 (= G165), G164 (= G166), M168 (≠ I170), E200 (= E202), K201 (= K203), Y202 (≠ G204), I203 (≠ L205), H208 (= H210), Q232 (= Q234), N235 (≠ H237), E275 (= E276), L277 (= L278), E287 (= E289), T443 (= T439)
binding bicarbonate ion: R291 (= R293), Q293 (= Q295), V294 (= V296), E295 (= E297)
binding magnesium ion: E275 (= E276), E287 (= E289)

Sites not aligning to the query:

binding acetyl coenzyme *a: 459, 460, 461, 1016, 1017, 1018, 1041, 1045
binding magnesium ion: 520, 523, 754
binding manganese (ii) ion: 535, 704, 733, 735
binding pyruvic acid: 538, 572, 605, 607, 704, 868

8hz4A The tetrameric structure of biotin carboxylase from chloroflexus aurantiacus in complex with bicarbonate (see paper)
51% identity, 95% coverage: 1:455/480 of query aligns to 1:450/456 of 8hz4A

query
sites
8hz4A

M

M

F

F

K

R

K

T

L

I

L

L

V

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

V

M

M

R

R

S

A

A

C

Q

R

E

E

L

L

G

G

I

L

R

R

T

C

V

V

A

A

I

V

Y
|
Y

E

S

E

E

T

A

D

D

K
x
R

T

D

A

A

M

P

H

H

I

V

M

A

K

Y

S

A

D

D

E
x
D

A
|
A

V
x
F

C
x
L

I

I

G

G

S

P

-

P

G

S

P

P

R

A

K

E

D

S

Y

Y

L

L

D

N

I

I

D

D

R

A

I

I

Q

R

A

A

K

K

A

A

V

T

G

G

A

A

D

E

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

A

E

E

N

N

P

A

L

S

F

F

S

V

K

R

R

A

C

V

T

T

D

A

N

A

G

G

L

L

I

I

F

F

V

I

G

G

P

P

P

A

Q

E

V

A

I

M

S

E

D

R

M

M

G

G

S

G

K

K

V

T

I

A

A

A

R

R

K

R

V

E

M

A

T

T

E

A

A

A

G

G

I

V

P

P

V

V

I

V

P

P

G

G

S

-

G

-

V

V

L

L

A

E

E

P

G

V

Q

T

D

D

G

A

E

-

K

A

E

E

A

V

L

R

E

R

F

L

A

G

R

K

L

E

H

F

G

G

F

Y

P

P

I

I

M

A

V

I

K

K

A

A

V

V

A

-

G

-

G

G

R

R

G

G

I

L

R

R

A

V

V

V

K

R

D

S

T

P

A

E

E

E

L

V

I

D

K

E

G

A

L

F

R

A

L

A

A

A

R

R

S

R

E

E

A

A

R

E

M

V

A

A

F

F

G

K

D

N

D

G

R

E

I

L

Y

Y

L

V

E

E

K

K

G

Y

L

L

Q

D

N

D

P

P

R

R

H

H

V

I

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

R

H

Y

G

G

N

N

V

A

I

V

H

A

L

L

G

G

T

E

R

R

N

D

C

C

S

S

I

V

Q

Q

R

R

H

H

Q

Q

K

K

M

L

I

I

E

E

I

E

A

C

P

P

A

S

S

-

L

-

P

P

A

A

L

L

V

T

E

P

E

E

-

L

-

R

-

A

R

E

I

M

C

G

A

A

D

A

A

A

V

V

R

R

A

L

A

A

Q

K

A

A

T

V

D

G

Y

Y

V

V

N

S

A

A

G

G

T

T

V

L

E

E

F

F

L

L

V

F

D

Q

S

-

E

D

D

G

Q

R

Y

Y

F

F

L

L

E

E

V

M

N

N

T

T

R
|
R

I

I

Q
|
Q

V

V

E
|
E

H

H

T

T

V

V

T

T

E

E

M

M

V

V

T

Y

G

G

V

I

D

D

I

L

V

V

R

A

E

A

Q

Q

L

I

R

R

I

I

A

A

A

Q

G

G

E

E

P

K

L

L

S

W

F

F

R

K

Q

Q

E

E

D

D

V

V

Q

V

V

P

R

R

G

G

Y

H

A

A

I

I

E

E

L

C

R

R

I

I

N

N

A

A

E

E

D

D

P

P

K

L

N

H

G

N

F

F

M

R

P

P

S

A

P

L

G

G

L

T

I

I

R

G

I

E

Y

Y

Q

H

S

E

P

P

G

V

G

G

H

F

G

G

V

V

R

R

L

V

D

D

G

S

C

G

I

V

Y

R

Q

A

G

Y

Y

Y

E

T

I

V

P

P

R

S

Y

H

Y

Y

D

D

S

S

M

L

L

L

V

A

K

K

L

L

T

I

V

T

Y

W

G

G

F

S

T

D

W

R

R

Q

E

E

A

A

V

I

D

A

R

R

L

M

R

R

A

A

L

L

D

A

G

E

F

Y

S

R

I

I

V

E

G

G

V

V

K

T

T

T

T

I

I

I

P

P

F

F

Y

H

K

Q

Q

A

I

A

V

L

E

E

D

H

P

P

D

V

F

F

I

T

H

A

Q

G

N

A

F

A

D

T

T

V

S

N

Y

F

I

I

D

P

T

R

H

H

P

P

Q

E

L

L

L

F

D

S

Y

R

R

A

E

A

E

E

V

L

binding bicarbonate ion: R288 (= R293), Q290 (= Q295), E292 (= E297)
binding : Y32 (= Y32), R37 (≠ K37), D47 (≠ E47), A48 (= A48), F49 (≠ V49), L50 (≠ C50)

P24182 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Escherichia coli (strain K12) (see 3 papers)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/449 of P24182

query
sites
P24182

M

M

F

L

K

D

K

K

L

I

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R
|
R

S

A

A

C

Q

K

E
|
E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

V

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

S

A

A

K

E

A

I

V

T

G

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

N

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

I

F

F

V

I

G

G

P

P

P

K

P

A

Q

E

V

T

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

A

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

G

E

D

G

D

Q

M

D

D

G

K

E

N

K

R

E

A

A

-

L

-

E

-

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

V

M

I

V

I

K
|
K

A

A

V

S

A

G

G

G

G
|
G

R

R

G

G

I

M

R

R

A

V

V

V

K

R

D

G

T

D

A

A

E

E

L

L

I

A

K

Q

G

S

L

I

R

S

L

M

A

T

R

R

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

S

D

N

D

D

R

M

I

V

Y

Y

L

M

E
|
E

K
|
K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

H
|
H

Q

Q

K
|
K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

P

E

E

-

L

-

R

R

Y

I

I

C

G

A

E

D

R

A

C

V

A

R

K

A

A

A

C

Q

V

A

D

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T

T

V

F

E
|
E

F

F

L

L

V

F

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N

N

T

T

R
|
R

I

I

Q

Q

V
|
V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

I

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

Q

P

P

L

L

S

S

F

I

R

K

Q

Q

E

E

D

E

V

V

Q

H

V

V

R

R

G

G

Y

H

A

A

I

V

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

-

N

N

G

T

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

I

R

T

I

R

Y

F

Q

H

S

A

P

P

G

G

H
x
F

G

G

V

V

R
|
R

L

W

D

E

G

S

C

H

I

I

Y

Y

Q

A

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

Y

D

D

S

S

M

M

L

I

V

G

K

K

L

L

T

I

V

C

Y

Y

G

G

F

E

T

N

W

R

R

D

E

V

A

A

V

I

D

A

R

R

L

M

R

K

R

N

A

A

L

L

D

Q

G

E

F

L

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

V

P

D

F

L

Y

Q

K

I

Q

R

I

I

V

M

E

N

D

D

P

E

D

N

F

F

I

Q

H

H

Q

G

N

G

F

T

D

N

T

I

S

H

Y

Y

I

L

D

E

R19 (= R19) mutation to E: Loss of homodimerization. No effect on ATP binding.
E23 (= E23) mutation to R: Loss of homodimerization. No effect on ATP binding.
K116 (= K115) binding ATP
K159 (= K160) binding ATP
GG 165:166 (= GG 166:167) binding ATP
EKYL 201:204 (≠ EKGL 202:205) binding ATP
H209 (= H210) binding ATP
H236 (= H237) binding ATP
K238 (= K239) binding hydrogencarbonate
E276 (= E276) binding ATP; binding Mg(2+)
E288 (= E289) binding ATP; binding Mg(2+)
R292 (= R293) active site; binding hydrogencarbonate
V295 (= V296) binding hydrogencarbonate
E296 (= E297) mutation to A: Severe reduction in catalytic activity.
R338 (= R339) binding biotin; binding hydrogencarbonate; mutation to A: Severe reduction in catalytic activity.
F363 (≠ H365) mutation to A: Loss of homodimerization. No effect on ATP binding.
R366 (= R368) mutation to E: Loss of homodimerization. No effect on ATP binding.

3jzfB Crystal structure of biotin carboxylase from e. Coli in complex with benzimidazoles series (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 3:443/447 of 3jzfB

query
sites
3jzfB

M

M

F

L

K

D

K

K

L

I

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

V

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

S

A

A

K

E

A

I

V

T

G

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

A

S

E

E

N

N

P

A

L

N

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

I

F

F

V

I

G

G

P

P

P

K

P

A

Q

E

V

T

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

A

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

G

E

D

G

D

Q

M

D

D

G

K

E

N

K

R

E

A

A

-

L

-

E

-

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

V

M

I

V

I

K
|
K

A
|
A

V

S

A

G

G

G

G
|
G

G

G

G
|
G

R
|
R

G
|
G

I
x
M

R

R

A

V

V

V

K

R

D

G

T

D

A

A

E

E

L

L

I

A

K

Q

G

S

L

I

R

S

L

M

A

T

R

R

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

S

D

N

D
|
D

R

M

I

V

Y
|
Y

L

M

E
|
E

K
|
K

G
x
Y

L

L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q

Q

R

R

R
|
R

H
|
H

Q

Q

K

K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

P

E

E

-

L

-

R

R

Y

I

I

C

G

A

E

D

R

A

C

V

A

R

K

A

A

A

C

Q

V

A

D

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

F

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L
x
I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

I

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

Q

P

P

L

L

S

S

F

I

R

K

Q

Q

E

E

D

E

V

V

Q

H

V

V

R

R

G

G

Y

H

A

A

I

V

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

-

N

N

G

T

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

I

R

T

I

R

Y

F

Q

H

S

A

P

P

G

G

H

F

G

G

V

V

R

R

L

W

D

E

G

S

C

H

I

I

Y

Y

Q

A

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

Y

D

D

S

S

M

M

L

I

V

G

K

K

L

L

T

I

V

C

Y

Y

G

G

F

E

T

N

W

R

R

D

E

V

A

A

V

I

D

A

R

R

L

M

R

K

R

N

A

A

L

L

D

Q

G

E

F

L

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

V

P

D

F

L

Y

Q

K

I

Q

R

I

I

V

M

E

N

D

D

P

E

D

N

F

F

I

Q

H

H

Q

G

N

G

F

T

D

N

T

I

S

H

Y

Y

I

L

D

E

active site: K118 (= K115), K161 (= K160), D198 (= D197), H211 (= H210), R237 (= R236), T276 (= T274), E278 (= E276), E290 (= E289), N292 (= N291), R294 (= R293), E298 (= E297), R340 (= R339)
binding 2-[(2-chlorobenzyl)amino]-1-(cyclohexylmethyl)-1H-benzimidazole-5-carboxamide: K118 (= K115), K161 (= K160), A162 (= A161), G166 (= G165), G168 (= G167), R169 (= R168), G170 (= G169), M171 (≠ I170), Y201 (= Y200), E203 (= E202), K204 (= K203), Y205 (≠ G204), H211 (= H210), H238 (= H237), L280 (= L278), I289 (≠ L288), E290 (= E289)

3rupA Crystal structure of e.Coli biotin carboxylase in complex with two adp and two ca ions (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/444 of 3rupA

query
sites
3rupA

M

M

F

L

K

D

K

K

L

I

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

V

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

S

A

A

K

E

A

I

V

T

G

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y
|
Y

G
|
G

F

F

L

L

A

S

E
|
E

N

N

P

A

L

N

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

I

F

F

V

I

G

G

P

P

P

K

P

A

Q

E

V

T

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

A

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

G

E

D

G

D

Q

M

D

D

G

K

E

N

K

R

E

A

A

-

L

-

E

-

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

V

M

I

V

I

K
|
K

A

A

V

S

A

G

G

G

G
|
G

R
|
R

G

G

I
x
M

R

R

A

V

V

V

K

R

D

G

T

D

A

A

E

E

L

L

I

A

K

Q

G

S

L

I

R

S

L

M

A

T

R

R

S

A

E

E

A

A

R

K

M

A

A

A

F
|
F

G

S

D

N

D
|
D

R

M

I

V

Y

Y

L

M

E
|
E

K
|
K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q
|
Q

R

R

R
|
R

H
|
H

Q

Q

K
|
K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

P

E

E

-

L

-

R

R

Y

I

I

C

G

A

E

D

R

A

C

V

A

R

K

A

A

A

C

Q

V

A

D

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

F

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V
|
V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

I

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

Q

P

P

L

L

S

S

F

I

R

K

Q

Q

E

E

D

E

V

V

Q

H

V

V

R

R

G

G

Y

H

A

A

I

V

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

-

N

N

G

T

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

I

R

T

I

R

Y

F

Q

H

S

A

P

P

G

G

H

F

G

G

V

V

R

R

L

W

D

E

G

S

C

H

I

I

Y

Y

Q

A

G

G

Y

Y

E

T

I

V

P

P

R

P

Y

Y

D
|
D

S

S

M

M

L

I

V

G

K

K

L

L

T

I

V

C

Y

Y

G

G

F

E

T

N

W

R

R

D

E

V

A

A

V

I

D

A

R

R

L

M

R

K

R

N

A

A

L

L

D

Q

G

E

F

L

S

I

I

I

V

D

G

G

V

I

K

K

T

T

T

N

I

V

P

D

F

L

Y

Q

K

I

Q

R

I

I

V

M

E

N

D

D

P

E

D

N

F

F

I

Q

H

H

Q

G

N

G

F

T

D

N

T
x
I

S

H

Y

Y

I

L

D

E

active site: K116 (= K115), K159 (= K160), D196 (= D197), H209 (= H210), R235 (= R236), T274 (= T274), E276 (= E276), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
binding adenosine-5'-diphosphate: Y82 (= Y81), G83 (= G82), K116 (= K115), K159 (= K160), G164 (= G165), G164 (= G165), G165 (= G166), G166 (= G167), R167 (= R168), M169 (≠ I170), F193 (= F194), E201 (= E202), K202 (= K203), Y203 (≠ G204), L204 (= L205), H209 (= H210), Q233 (= Q234), H236 (= H237), K238 (= K239), L278 (= L278), E288 (= E289), R292 (= R293), V295 (= V296), E296 (= E297), R338 (= R339), D382 (= D384), I437 (≠ T439)
binding calcium ion: E87 (= E86), E276 (= E276), E288 (= E289), E288 (= E289), N290 (= N291)

3g8cA Crystal structure of biotin carboxylase in complex with biotin, bicarbonate, adp and mg ion (see paper)
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/444 of 3g8cA

query
sites
3g8cA

M

M

F

L

K

D

K

K

L

I

L

V

V

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

I

L

R

R

I

I

M

L

R

R

S

A

A

C

Q

K

E

E

L

L

G

G

I

I

R

K

T

T

V

V

A

A

I

V

Y

H

E

S

T

A

D

D

K

R

T

D

A

L

M

K

H

H

I

V

M

L

K

L

S

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

S

P

G

A

P

P

R

S

-

V

K

K

D

S

Y

Y

L

L

D

N

I

I

D

P

R

A

I

I

Q

S

A

A

K

E

A

I

V

T

G

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y
|
Y

G

G

F
|
F

L

L

A

S

E

E

N

N

P

A

L

N

F

F

S

A

K

E

R

Q

C

V

T

E

D

R

N

S

G

G

L

F

I

I

F

F

V

I

G

G

P

P

P

K

P

A

Q

E

V

T

I

I

S

R

D

L

M

M

G

G

S

D

K
|
K

V

V

I

S

A

A

R

I

K

A

V

A

M

M

T

K

E

K

A

A

G

G

I

V

P

P

V

C

I

V

P

P

G

G

S

S

-

D

G

G

V

P

L

L

A

G

E

D

G

D

Q

M

D

D

G

K

E

N

K

R

E

A

A

-

L

-

E

-

F

I

A

A

R

K

L

R

H

I

G

G

F

Y

P

P

I

V

M
x
I

V

I

K
|
K

A

A

V

S

A

G

G

G

G
|
G

G

G

R

R

G

G

I
x
M

R

R

A

V

V

V

K

R

D

G

T

D

A

A

E

E

L

L

I

A

K

Q

G

S

L

I

R

S

L

M

A

T

R

R

S

A

E

E

A

A

R

K

M

A

A

A

F

F

G

S

D

N

D
|
D

R

M

I

V

Y

Y

L

M

E
|
E

K
|
K

G
x
Y

L
|
L

Q

E

N

N

P

P

R

R

H
|
H

V

V

E

E

V

I

Q

Q

I

V

L

L

A

A

D

D

G

G

H

Q

G

G

N

N

V

A

I

I

H

Y

L

L

G

A

T

E

R

R

N

D

C

C

S

S

I

M

Q
|
Q

R

R

R
|
R

H
|
H

Q

Q

K
|
K

M

V

I

V

E

E

I

E

A

A

P

P

A

A

S

-

L

-

P

P

A

G

L

I

V

T

E

P

E

E

-

L

-

R

R

Y

I

I

C

G

A

E

D

R

A

C

V

A

R

K

A

A

A

C

Q

V

A

D

T

I

D

G

Y

Y

V

R

N

G

A

A

G

G

T
|
T

V

F

E
|
E

F

F

L
|
L

V

F

D

E

S

N

E

-

D

G

Q

E

Y

F

Y

Y

F

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q
|
Q

V
|
V

E
|
E

H

H

T

P

V

V

T

T

E

E

M

M

V

I

T

T

G

G

V

V

D

D

I

L

V

I

R

K

E

E

Q

Q

L

L

R

R

I

I

A

A

G

G

E

Q

P

P

L

L

S

S

F

I

R

K

Q

Q

E

E

D

E

V

V

Q

H

V

V

R

R

G

G

Y

H

A

A

I

V

E

E

L

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

K

-

N

N

G

T

F

F

M

L

P

P

S

S

P

P

G

G

L

K

I

I

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D

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M

M

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K

K

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Y

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F

E

T

N

W

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A

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I

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M

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N

A

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K

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active site: K116 (= K115), K159 (= K160), D196 (= D197), H209 (= H210), R235 (= R236), T274 (= T274), E276 (= E276), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
binding adenosine-5'-diphosphate: I157 (≠ M158), K159 (= K160), G164 (= G165), M169 (≠ I170), E201 (= E202), K202 (= K203), Y203 (≠ G204), L204 (= L205), Q233 (= Q234), H236 (= H237), L278 (= L278), E288 (= E289), I437 (≠ T439)
binding bicarbonate ion: K238 (= K239), R292 (= R293), Q294 (= Q295), V295 (= V296), E296 (= E297)
binding biotin: Y82 (= Y81), F84 (= F83), R292 (= R293), V295 (= V296), R338 (= R339), D382 (= D384)
binding magnesium ion: E276 (= E276), E288 (= E289)

8uz2C E. Coli acetyl-coa carboxylase, narrow helical local reconstruction, 3.18 angstrom
49% identity, 92% coverage: 1:443/480 of query aligns to 1:441/446 of 8uz2C

query
sites
8uz2C

M

M

F

L

K

D

K

K

L

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L

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A

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C

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G

A

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-

V

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A

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H

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P

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Y

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F

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L

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E

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N

P

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G

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x
M

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A

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H

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binding adenosine-5'-diphosphate: K116 (= K115), K159 (= K160), G164 (= G165), G165 (= G166), G166 (= G167), M169 (≠ I170), H209 (= H210), Q233 (= Q234), H236 (= H237), L278 (= L278), E288 (= E289), I437 (≠ T439)
binding magnesium ion: E276 (= E276), E288 (= E289)
binding : R356 (≠ I358), I410 (≠ S412)

Query Sequence

>WP_073038629.1 NCBI__GCF_900129305.1:WP_073038629.1
MFKKLLVANRGEIAIRIMRSAQELGIRTVAIYEETDKTAMHIMKSDEAVCIGSGPRKDYL
DIDRIIQAAKAVGADAIHPGYGFLAENPLFSKRCTDNGLIFVGPPPQVISDMGSKVIARK
VMTEAGIPVIPGSGVLAEGQDGEKEALEFARLHGFPIMVKAVAGGGGRGIRAVKDTAELI
KGLRLARSEARMAFGDDRIYLEKGLQNPRHVEVQILADGHGNVIHLGTRNCSIQRRHQKM
IEIAPASLPALVEERICADAVRAAQATDYVNAGTVEFLVDSEDQYYFLEVNTRIQVEHTV
TEMVTGVDIVREQLRIAAGEPLSFRQEDVQVRGYAIELRINAEDPKNGFMPSPGLIRIYQ
SPGGHGVRLDGCIYQGYEIPRYYDSMLVKLTVYGFTWREAVDRLRRALDGFSIVGVKTTI
PFYKQIVEDPDFIHQNFDTSYIDTHPQLLDYREEVPELDRLARLIAEINAYGYNPYAESA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory