SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_073039068.1 NCBI__GCF_900129305.1:WP_073039068.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O52681 Bifurcating [FeFe] hydrogenase gamma subunit; Hydrogenase (NAD(+), ferredoxin) gamma subunit; Iron-hydrogenase gamma subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
40% identity, 99% coverage: 1:154/156 of query aligns to 1:154/161 of O52681

query
sites
O52681

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D79 (≠ T79) mutation to P: Increase (30 mV) in the redox potential.
G80 (= G80) mutation to P: Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
T81 (= T81) mutation to P: Small increase (11 mV) in the redox potential.
A82 (= A82) mutation to P: Decrease (46 mV) in the redox potential.
L120 (≠ V120) mutation to P: Large decrease (65 mV) in the redox potential.
G121 (= G121) mutation to P: Decrease (22 mV) in the redox potential.
A122 (= A122) mutation to P: Large decrease (132 mV) in the redox potential.
V128 (= V128) mutation V->A,C,H,Y: Small increase (about 2-14 mV) in the redox potential.; mutation V->D,G,R,S,T,W: Increase (about 23-33 mV) in the redox potential.; mutation V->E,K,L,M: Decrease (about 37-50 mV) in the redox potential.; mutation V->F,I,P,Q: Small decrease (about 4-20 mV) in the redox potential.; mutation to N: Large increase (85 mV) in the redox potential.

8a6tC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
45% identity, 94% coverage: 5:150/156 of query aligns to 4:149/151 of 8a6tC

query
sites
8a6tC

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binding fe2/s2 (inorganic) cluster: C77 (= C78), G79 (= G80), A81 (= A82), C82 (= C83), C118 (= C119), L119 (≠ V120), C122 (= C123)

7p5hC Tmhydabc- d2 map (see paper)
40% identity, 96% coverage: 5:154/156 of query aligns to 2:151/156 of 7p5hC

query
sites
7p5hC

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binding fe2/s2 (inorganic) cluster: C75 (= C78), G77 (= G80), A79 (= A82), C80 (= C83), C116 (= C119), L117 (≠ V120), A119 (= A122), C120 (= C123)

7t2rC Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn-free apo state (see paper)
42% identity, 94% coverage: 3:148/156 of query aligns to 3:148/152 of 7t2rC

query
sites
7t2rC

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binding fe2/s2 (inorganic) cluster: C78 (= C78), C83 (= C83), C119 (= C119), L120 (≠ V120), G121 (= G121), A122 (= A122), C123 (= C123), V128 (= V128)

8a5eC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
35% identity, 94% coverage: 5:150/156 of query aligns to 10:155/156 of 8a5eC

query
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8a5eC

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binding fe2/s2 (inorganic) cluster: C83 (= C78), G85 (= G80), A87 (= A82), C88 (= C83), R123 (≠ N118), C124 (= C119), C128 (= C123)

8oh5A Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
34% identity, 92% coverage: 8:151/156 of query aligns to 6:149/150 of 8oh5A

query
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8oh5A

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binding fe2/s2 (inorganic) cluster: C76 (= C78), C81 (= C83), C117 (= C119), L118 (≠ V120), G119 (= G121), C120 (≠ A122), C121 (= C123)

8eswV2 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
33% identity, 88% coverage: 16:152/156 of query aligns to 38:174/214 of 8eswV2

query
sites
8eswV2

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binding fe2/s2 (inorganic) cluster: C100 (= C78), C105 (= C83), C141 (= C119), L142 (≠ V120), G143 (= G121), C145 (= C123)
binding : R53 (≠ H31)

Sites not aligning to the query:

binding : 27, 30, 37

8b9zE Drosophila melanogaster complex i in the active state (dm1) (see paper)
33% identity, 88% coverage: 16:152/156 of query aligns to 38:174/214 of 8b9zE

query
sites
8b9zE

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E

R

T

M

C

K

K

Q

K

D

Q

L

L

G

G

L

I

D

G

P

V

G

G

E

D

T

T

E

K

D

D

L

R

A

K

L

F

T

T

L

I

E

S

T

E

V

V

N

E

C
|
C

V
x
L

G
|
G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

A

V

I

N

N

G

D

E

D

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

S

N

K

K

D

M

M

A

Q

A

D

L

I

V

L

K

N

E

D

I

L

K

K

binding fe2/s2 (inorganic) cluster: C100 (= C78), T102 (≠ G80), T103 (= T81), P104 (≠ A82), C105 (= C83), C141 (= C119), L142 (≠ V120), G143 (= G121), A144 (= A122), C145 (= C123)

5gupE structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
32% identity, 93% coverage: 8:152/156 of query aligns to 41:187/197 of 5gupE

query
sites
5gupE

V

I

D

E

A

A

V

I

I

V

D

K

Q

N

W

Y

-

P

-

E

G

G

A

H

K

K

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H

R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

I

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C
|
C

T

T

G
x
T

T

T

A
x
P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

K

S

L

I

L

L

D

E

R

A

M

I

K

Q

Q

K

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C
|
C

V
x
L

G
|
G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V
x
M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

binding fe2/s2 (inorganic) cluster: C113 (= C78), T115 (≠ G80), P117 (≠ A82), C118 (= C83), C154 (= C119), L155 (≠ V120), G156 (= G121), A157 (= A122), C158 (= C123), M163 (≠ V128)

7dgq9 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
32% identity, 93% coverage: 8:152/156 of query aligns to 24:170/207 of 7dgq9

query
sites
7dgq9

V

I

D

E

A

A

V

I

I

V

D
x
K

Q

N

W

Y

-
x
P

-

E

G

G

A
x
H

K

K

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H
x
R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

I

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C
|
C

T

T

G
x
T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

K

S

L

I

L

L

D

E

R

A

M

I

K

Q

Q

K

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C
|
C

V
x
L

G

G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

binding fe2/s2 (inorganic) cluster: C96 (= C78), T98 (≠ G80), C101 (= C83), C137 (= C119), L138 (≠ V120), A140 (= A122), C141 (= C123)
binding : K29 (≠ D13), P32 (vs. gap), H35 (≠ A17), R49 (≠ H31)

Sites not aligning to the query:

binding : 20, 23

P04394 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase subunit II; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Bos taurus (Bovine) (see 2 papers)
32% identity, 93% coverage: 8:152/156 of query aligns to 63:209/249 of P04394

query
sites
P04394

V

I

D

E

A

A

V

I

I

V

D

K

Q

N

W

Y

-

P

-

E

G

G

A

H

K

K

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H

R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

I

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C
|
C

T

T

G

T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

K

S

L

I

L

L

D

E

R

A

M

I

K

Q

Q

K

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C
|
C

V

L

G

G

A

A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

C135 (= C78) binding [2Fe-2S] cluster
C140 (= C83) binding [2Fe-2S] cluster
C176 (= C119) binding [2Fe-2S] cluster
C180 (= C123) binding [2Fe-2S] cluster

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

7v2cO Active state complex i from q10 dataset (see paper)
32% identity, 93% coverage: 8:152/156 of query aligns to 31:177/217 of 7v2cO

query
sites
7v2cO

V

I

D

E

A

A

V

I

I

V

D

K

Q

N

W

Y

-

P

-

E

G

G

A

H

K

K

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H

R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

I

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C
|
C

T

T

G

T

T

T

A
x
P

C
|
C

H

M

V

L

K

R

G

N

A

S

P

D

K

S

L

I

L

L

D

E

R

A

M

I

K

Q

Q

K

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C
|
C

V
x
L

G
|
G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

binding fe2/s2 (inorganic) cluster: C103 (= C78), P107 (≠ A82), C108 (= C83), C144 (= C119), L145 (≠ V120), G146 (= G121), A147 (= A122), C148 (= C123)

Q9D6J6 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Mus musculus (Mouse) (see paper)
31% identity, 93% coverage: 8:152/156 of query aligns to 62:208/248 of Q9D6J6

query
sites
Q9D6J6

V

I

D

E

A

A

V

I

I

V

D

K

Q

N

W

Y

-

P

-

E

G

G

A

H

K

Q

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H

R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

V

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C
|
C

T

T

G

T

T

T

A

P

C
|
C

H

M

V

L

K

R

G

D

A

S

P

D

K

S

L

I

L

L

D

E

R

T

M

L

K

Q

Q

R

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C
|
C

V

L

G

G

A

A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

C134 (= C78) binding [2Fe-2S] cluster
C139 (= C83) binding [2Fe-2S] cluster
C175 (= C119) binding [2Fe-2S] cluster
C179 (= C123) binding [2Fe-2S] cluster

P19404 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NDUFV2; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see 2 papers)
31% identity, 93% coverage: 8:152/156 of query aligns to 63:209/249 of P19404

query
sites
P19404

V

I

D

E

A

A

V

I

I

V

D

K

Q

N

W

Y

-

P

-

E

G

G

A

H

K

K

P

A

E

A

S

A

L

V

L

L

Q

P

I

V

M

L

L

D

D

L

V

A

N

Q

H

R

H

Q

F

N

N

G

Y

W

L

L

P

P

R

I

E

S

S

A

I

M

V

N

R

K

I

V

A

A

E

E

R

V

L

L

R

Q

M

V

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

K

P

P

R

V

G

G

R

K

T

Y

I

H

V

I

H

Q

V

V

C

C

T

T

G

T

T

T

A

P

C

C

H

M

V

L

K

R

G

N

A

S

P

D

K

S

L

I

L

L

D

E

R

A

M

I

K

Q

Q

K

D

K

L

L

G

G

L

I

D

K

P

V

G

G

E

E

T

T

E

P

D

D

L

K

A

L

L

F

T

T

L

L

E

I

T

E

V

V

N

E

C

C

V

L

G

G

A

A

C

C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

N

T

Y

Y
|
Y

S

E

E

D

V

L

T

T

P

A

N

K

K

D

M

I

A

E

L

I

V

I

K

D

E

E

I

L

K

K

Y193 (= Y136) modified: Phosphotyrosine; by SRC

Sites not aligning to the query:

29 V → A: in dbSNP:rs906807

7b0nE 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
38% identity, 70% coverage: 44:152/156 of query aligns to 66:174/216 of 7b0nE

query
sites
7b0nE

I

V

A

A

E

K

R

L

L

L

R

E

M

M

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

T

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

T

P

P

R

M

G

G

R

R

T

Y

I

H

V

L

H

Q

V

I

C
|
C

T

T

G
x
T

T

T

A
x
P

C
|
C

H

Q

V

L

K

C

G

G

A

S

P

D

K

G

L

I

L

M

D

E

R

A

M

V

K

Q

Q

N

D

T

L

L

G

N

L

I

D

K

P

P

G

G

E

E

T

T

E

K

D

D

L

N

A

L

L

F

T

T

L

L

E

S

T

E

V

V

N

E

C
|
C

V
x
L

G

G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

A

V

I

N

N

G

D

E

D

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

E

K

G

M

T

A

V

A

K

L

L

V

L

K

E

E

D

I

C

K

K

binding fe2/s2 (inorganic) cluster: C100 (= C78), T102 (≠ G80), P104 (≠ A82), C105 (= C83), C141 (= C119), L142 (≠ V120), A144 (= A122), C145 (= C123)

8e9gE Mycobacterial respiratory complex i with both quinone positions modelled (see paper)
35% identity, 99% coverage: 2:155/156 of query aligns to 26:179/233 of 8e9gE

query
sites
8e9gE

E

E

N

S

I

L

L

R

Q

A

D

D

V

A

D

E

A

Q

V

I

I

I

D

A

Q

R

W

Y

G

P

A

D

K

A

P

R

E

S

S

A

L

L

Q

P

I

L

M

L

L

H

D

L

V

V

N

Q

H

A

H

Q

F

D

N

G

Y

Y

L

L

P

T

R

P

E

A

S

G

I

I

V

G

R

F

I

C

A

A

E

A

R

Q

L

L

R

G

M

L

P

T

V

E

S

A

Q

E

V

V

Y

T

S

A

V

V

A

A

T

T

F

F

F

Y

K

S

V

M

F

Y

S

R

L

R

T

T

P

P

R

T

G

G

R

D

T

Y

I

L

V

V

H

G

V

V

C
|
C

T

T

G

N

T

T

A

L

C
|
C

H

A

V

I

K

M

G

G

A

G

P

D

K

A

L

I

L

L

D

E

R

A

M

L

K

E

Q

D

D

H

L

L

G

G

L

V

D

H

P

P

G

G

E

Q

T

T

E

P

D

D

L

G

A

R

L

V

T

T

L

L

E

E

T

H

V

V

N

E

C
|
C

V
x
N

G
x
A

A
|
A

C
|
C

A

D

S

Y

A

A

P

P

V
|
V

V

V

V

M

V

V

N

N

G

W

E

E

T

F

Y

Y

S

D

E

N

V

Q

T

T

P

P

N

S

K

S

M

A

A

R

A

D

L

L

V

V

K

D

E

G

I

L

K

R

E

S

Q

G

S

S

binding fe2/s2 (inorganic) cluster: C102 (= C78), C107 (= C83), C143 (= C119), N144 (≠ V120), A145 (≠ G121), A146 (= A122), C147 (= C123), V152 (= V128)

6r7pA Crystal structure of oxidized aquifex aeolicus nadh-quinone oxidoreductase subunits nuoe and nuof s96m (see paper)
32% identity, 87% coverage: 12:146/156 of query aligns to 17:154/157 of 6r7pA

query
sites
6r7pA

I

I

D

N

Q

Y

W

F

G

P

A

K

K

K

P

R

E

Q

S

A

L

I

L

L

Q

L

I

C

M

L

L

H

D

E

V

I

N

Q

H

N

H

Y

F

Y

N

G

Y

Y

L

I

P

P

R

P

E

E

S

S

I

L

V

K

R

P

I

L

A

A

E

D

R

M

L

L

R

E

M

L

P

P

V

L

S

N

Q

H

V

V

Y

E

S

G

V

V

A

V

T

A

F

F

F

Y

K

D

V

M

F

F

S

D

L

R

T

E

P

D

R

K

G

A

R

K

T

Y

I

R

V

I

H

R

V

V

C
|
C

T

V

G

S

T

I

A
x
V

C
|
C

H

H

V

L

K

M

G

G

A

T

P

N

K

K

L

L

D

K

R

A

M

L

K

E

Q

N

D

I

L

L

G

G

L

I

D

K

P

P

G

G

E

E

T

V

T

T

E

P

D

D

L

G

A

K

L

F

T

K

L

I

E

V

T

P

V

V

N

Q

C
|
C

V
x
L

G

G

A
|
A

C
|
C

A

S

S

E

A

A

P

P

V

V

V

F

V

M

V

V

N

N

G

D

E

D

T

E

Y

Y

-

K

-

F

-

E

S

S

E

E

V

V

T

Q

P

L

N

N

K

E

M

I

A

L

A

S

binding fe2/s2 (inorganic) cluster: C83 (= C78), V87 (≠ A82), C88 (= C83), C124 (= C119), L125 (≠ V120), A127 (= A122), C128 (= C123)

6vw8C Formate dehydrogenase fdsabg subcomplex fdsbg from c. Necator (see paper)
35% identity, 87% coverage: 19:153/156 of query aligns to 17:151/152 of 6vw8C

query
sites
6vw8C

P

P

E

G

S

A

L

L

Q

P

I

I

M

L

L

H

D

E

V

I

N

Q

H

D

H

T

F

Q

N

G

Y

Y

L

I

P

P

R

D

E

A

S

A

I

V

V

P

R

V

I

I

A

A

E

R

R

A

L

L

R

N

M

L

P

S

V

R

S

A

Q

E

V

V

Y

H

S

G

V

V

A

I

T

T

F

F

F

Y

K

H

V

H

F

F

S

R

L

Q

T

Q

P

P

R

A

G

G

R

R

T

H

I

V

V

V

H

Q

V

V

C
|
C

T

R

G

A

T

E

A
|
A

C
|
C

H

Q

V

S

K

V

G

G

A

A

P

E

K

A

L

L

L

A

D

E

R

H

M

A

K

Q

Q

R

D

A

L

L

G

G

L

C

D

G

P

F

G

H

E

E

T

T

E

A

D

D

L

G

A

Q

L

V

T

T

L

L

E

E

T

P

V

V

N

Y

C
|
C

V
x
L

G
|
G

A
x
Q

C
|
C

A

A

S

C

A

G

P

P

V

A

V

V

V

M

V

V

N

G

G

E

E

Q

T

L

Y

H

S

G

E

Y

V

V

T

D

P

A

N

R

K

R

M

F

A

D

A

A

L

L

V

V

K

R

E

S

I

L

K

R

E

E

binding fe2/s2 (inorganic) cluster: C76 (= C78), A80 (= A82), C81 (= C83), C117 (= C119), L118 (≠ V120), G119 (= G121), Q120 (≠ A122), C121 (= C123)

P9WIV5 NADH-quinone oxidoreductase subunit E; NADH dehydrogenase I subunit E; NDH-1 subunit E; EC 7.1.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 96% coverage: 7:155/156 of query aligns to 43:191/252 of P9WIV5

query
sites
P9WIV5

D

D

V

A

D
x
K

A

E

V

I

I

I

D

G

Q

R

W

Y

G

P

A

D

K

R

P

R

E

S

S

A

L

L

Q

P

I

L

M

L

L

H

D

L

V

V

N

Q

H

G

H

E

F

D

N

S

Y

Y

L

L

P

T

R

P

E

A

S

G

I

L

V

R

R

F

I

C

A

A

E

D

R

Q

L

L

R

G

M

L

P

T

V

G

S

A

Q

E

V

V

Y

S

S

A

V

V

A

A

T

S

F

F

F

Y

K

T

V

M

F

Y

S

R

L

R

T

R

P

P

R

T

G

G

R

E

T

Y

I

L

V

V

H

G

V

V

C

C

T

T

G

N

T

T

A

L

C

C

H

A

V

V

K

M

G

G

A

G

P

D

K

A

L

I

L

F

D

D

R

R

M

L

K

K

Q

E

D

H

L

L

G

G

L

V

D

G

P

H

G

D

E

E

T

T

E

S

D

D

L

G

A

V

L

V

T

T

L

L

E

Q

T

H

V

I

N

E

C

C

V

N

G

A

A

A

C

C

A

D

S

Y

A

A

P

P

V

V

V

M

V

V

N

N

G

W

E

E

T

F

Y

F

S

D

E

N

V

Q

T

T

P

P

N

E

K

S

M

A

A

R

A

E

L

L

V

V

K

D

E

S

I

L

K

R

E

S

Q

D

S

T

K45 (≠ D9) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7zmbH Cryoem structure of mitochondrial complex i from chaetomium thermophilum (state 2) (see paper)
37% identity, 71% coverage: 44:153/156 of query aligns to 67:178/221 of 7zmbH

query
sites
7zmbH

I

V

A

A

E

R

R

I

L

L

R

E

M

M

P

P

V

P

S

M

Q

R

V

V

Y

Y

S

E

V

V

A

A

T

S

F

F

F

Y

K

T

V

M

F

Y

S

N

L

R

T

T

P

P

R

V

G

G

R

K

T

F

I

H

V

V

H

Q

V

A

C
|
C

T

T

G

T

T

T

A
x
P

C
|
C

H

Q

V

L

K

G

G

G

-

C

-

G

A

S

P

D

K

A

L

I

L

V

D

K

R

A

M

I

K

K

Q

E

D

H

L

L

G

G

L

I

D

N

P

Q

G

G

E

E

T

T

E

P

D

D

L

G

A

L

L

F

T

T

L

F

E

I

T

E

V

V

N

E

C
|
C

V

L

G

G

A
|
A

C
|
C

A

V

S

N

A

A

P

P

V

M

V

V

V

Q

V

I

N

N

G

D

E

D

T

Y

Y

Y

S

E

E

D

V

L

T

T

P

P

N

E

K

T

M

I

A

K

A

Q

L

V

V

L

K

T

E

A

I

L

K

K

E

E

binding fe2/s2 (inorganic) cluster: C101 (= C78), P105 (≠ A82), C106 (= C83), C144 (= C119), A147 (= A122), C148 (= C123)

Query Sequence

>WP_073039068.1 NCBI__GCF_900129305.1:WP_073039068.1
MENILQDVDAVIDQWGAKPESLLQIMLDVNHHFNYLPRESIVRIAERLRMPVSQVYSVAT
FFKVFSLTPRGRTIVHVCTGTACHVKGAPKLLDRMKQDLGLDPGETTEDLALTLETVNCV
GACASAPVVVVNGETYSEVTPNKMAALVKEIKEQSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory