SitesBLAST
Comparing WP_073040341.1 NCBI__GCF_900129305.1:WP_073040341.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4twrA Structure of udp-glucose 4-epimerase from brucella abortus
45% identity, 98% coverage: 2:312/318 of query aligns to 3:317/325 of 4twrA
- active site: S117 (= S116), C118 (≠ A117), A119 (= A118), Y141 (= Y140), K145 (= K144), H184 (= H183), H189 (= H188)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (≠ Y11), I13 (= I12), D32 (= D31), N33 (= N32), L34 (= L33), S35 (= S34), T36 (≠ N35), G37 (= G36), D51 (= D50), I52 (= I51), F73 (= F72), A74 (= A73), A75 (= A74), N92 (= N91), S115 (= S114), K145 (= K144), Y168 (= Y167), A171 (≠ V170), H184 (= H183)
- binding zinc ion: E182 (= E181), H184 (= H183), E187 (= E186), H189 (= H188)
2c20A Crystal structure of udp-glucose 4-epimerase
40% identity, 94% coverage: 2:299/318 of query aligns to 2:305/329 of 2c20A
- active site: T117 (≠ S116), A118 (= A117), A119 (= A118), Y141 (= Y140), K145 (= K144), H184 (= H183)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), Q34 (≠ S34), T35 (≠ N35), G36 (= G36), D51 (= D50), L52 (≠ I51), F73 (= F72), A74 (= A73), A75 (= A74), N92 (= N91), Y141 (= Y140), K145 (= K144), Y168 (= Y167), F169 (= F168), V171 (= V170), H184 (= H183)
- binding zinc ion: E182 (= E181), H184 (= H183), E187 (= E186), H189 (= H188)
7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
42% identity, 97% coverage: 1:310/318 of query aligns to 3:325/336 of 7kn1A
- active site: S126 (= S116), Y150 (= Y140), K154 (= K144)
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), S34 (≠ N32), C36 (≠ S34), N37 (= N35), D60 (= D50), I61 (= I51), F82 (= F72), A83 (= A73), A84 (= A74), K86 (≠ A76), S124 (= S114), S125 (= S115), S126 (= S116), Y150 (= Y140), K154 (= K144), Y178 (= Y167), P181 (≠ V170)
- binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (= V78), S126 (= S116), Y150 (= Y140), N180 (= N169), S199 (≠ T187), N200 (≠ H188), L201 (≠ V189), Q217 (≠ F204), V218 (≠ L205), F219 (= F206), R232 (= R219), Y234 (= Y221), V270 (= V256), R293 (= R279), D296 (= D282)
7k3pA The structure of the udp-glc/glcnac 4-epimerase from the human pathogen campylobacter jejuni
37% identity, 93% coverage: 3:299/318 of query aligns to 4:311/329 of 7k3pA
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), S35 (= S34), G37 (= G36), D57 (= D50), L58 (≠ I51), F79 (= F72), A80 (= A73), I83 (≠ A76), N98 (= N91), Y147 (= Y140), K151 (= K144), Y175 (= Y167), N177 (= N169), V178 (= V170)
Q9SA77 UDP-arabinose 4-epimerase 1; UDP-D-xylose 4-epimerase 1; EC 5.1.3.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 92% coverage: 2:294/318 of query aligns to 72:379/419 of Q9SA77
- G275 (≠ P191) mutation to D: In mur4-1; 50% reduction in L-Ara in cell wall material.
- R304 (= R219) mutation to Q: In mur4-3; 50% reduction in L-Ara in cell wall material.
2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 2udpA
- active site: S124 (= S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), S122 (= S114), S124 (= S116), Y149 (= Y140), K153 (= K144), Y177 (= Y167)
- binding phenyl-uridine-5'-diphosphate: N179 (= N169), N199 (≠ H188), L200 (≠ V189), A216 (≠ F204), I217 (≠ L205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)
1udcA Structure of udp-galactose-4-epimerase complexed with udp-mannose (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1udcA
- active site: S124 (= S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), S122 (= S114), Y149 (= Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding uridine-5'-diphosphate-mannose: T126 (≠ A118), Y149 (= Y140), N179 (= N169), N199 (≠ H188), L200 (≠ V189), L215 (≠ F203), A216 (≠ F204), I217 (≠ L205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279)
P09147 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Escherichia coli (strain K12) (see 10 papers)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of P09147
- YI 11:12 (= YI 11:12) binding NAD(+)
- DNLCNS 31:36 (≠ DNLSNG 31:36) binding NAD(+)
- DI 58:59 (= DI 50:51) binding NAD(+)
- FAGLK 80:84 (≠ FAALA 72:76) binding NAD(+)
- N99 (= N91) binding NAD(+)
- S124 (= S116) binding NAD(+); mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
- Y149 (= Y140) active site, Proton acceptor; binding NAD(+); mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
- K153 (= K144) binding NAD(+); mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
- F178 (= F168) binding NAD(+)
- Y299 (≠ L286) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.
1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1udaA
- active site: S124 (= S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), S122 (= S114), Y149 (= Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (≠ A118), N179 (= N169), N199 (≠ H188), L200 (≠ V189), A216 (≠ F204), I217 (≠ L205), F218 (= F206), R231 (= R219), Y233 (= Y221), R292 (= R279), D295 (= D282), Y299 (≠ L286)
1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1naiA
- active site: S124 (= S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), Y149 (= Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding 1,3-propandiol: N35 (= N35), K84 (≠ A76), E191 (= E181), P193 (= P185)
- binding uridine-5'-diphosphate: N179 (= N169), N199 (≠ H188), L200 (≠ V189), L215 (≠ F203), A216 (≠ F204), R231 (= R219), Y233 (= Y221), R292 (= R279)
1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1lrjA
- active site: S124 (= S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), Y149 (= Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding uridine-diphosphate-n-acetylglucosamine: V86 (= V78), S124 (= S116), F178 (= F168), N179 (= N169), L200 (≠ V189), L215 (≠ F203), A216 (≠ F204), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)
1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
41% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1kvrA
- active site: A124 (≠ S116), A125 (= A117), T126 (≠ A118), Y149 (= Y140), K153 (= K144), M189 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), S122 (= S114), S123 (= S115), Y149 (= Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding uridine-5'-diphosphate: N179 (= N169), N198 (≠ T187), N199 (≠ H188), L200 (≠ V189), A216 (≠ F204), I217 (≠ L205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)
1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
40% identity, 91% coverage: 1:289/318 of query aligns to 1:302/338 of 1a9yA
- active site: A124 (≠ S116), A125 (= A117), T126 (≠ A118), F149 (≠ Y140), K153 (= K144)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), C34 (≠ S34), N35 (= N35), S36 (≠ G36), D58 (= D50), I59 (= I51), F80 (= F72), A81 (= A73), G82 (≠ A74), K84 (≠ A76), S122 (= S114), S123 (= S115), F149 (≠ Y140), K153 (= K144), Y177 (= Y167), P180 (≠ V170)
- binding uridine-5'-diphosphate-glucose: A125 (= A117), T126 (≠ A118), N179 (= N169), N199 (≠ H188), L200 (≠ V189), A216 (≠ F204), I217 (≠ L205), F218 (= F206), R231 (= R219), Y233 (= Y221), R292 (= R279), D295 (= D282), Y299 (≠ L286)
2cnbA Trypanosoma brucei udp-galactose-4-epimerase in ternary complex with NAD and the substrate analogue udp-4-deoxy-4-fluoro-alpha-d-galactose (see paper)
37% identity, 94% coverage: 1:299/318 of query aligns to 3:343/366 of 2cnbA
- active site: S144 (= S116), A145 (= A117), A146 (= A118), Y169 (= Y140), K173 (= K144)
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D34 (= D31), S35 (≠ N32), V37 (vs. gap), G38 (vs. gap), D77 (= D50), V78 (≠ I51), M100 (≠ F72), C101 (≠ A73), A102 (= A74), L104 (≠ A76), N119 (= N91), S143 (= S115), S144 (= S116), Y169 (= Y140), K173 (= K144), Y196 (= Y167), F197 (= F168), A199 (≠ V170)
- binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: L104 (≠ A76), S144 (= S116), Y169 (= Y140), F197 (= F168), N198 (= N169), H217 (= H188), L218 (≠ V189), P238 (≠ F204), I239 (≠ L205), F240 (= F206), C251 (≠ A217), R253 (= R219), V297 (= V256), R320 (= R279), D323 (= D282)
1gy8C Trypanosoma brucei udp-galactose 4' epimerase (see paper)
37% identity, 94% coverage: 1:299/318 of query aligns to 3:347/370 of 1gy8C
- active site: S144 (= S116), A145 (= A117), A146 (= A118), Y170 (= Y140), K174 (= K144)
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D34 (= D31), S35 (≠ N32), L36 (= L33), V37 (vs. gap), G38 (vs. gap), D77 (= D50), V78 (≠ I51), M100 (≠ F72), C101 (≠ A73), A102 (= A74), L104 (≠ A76), N119 (= N91), S142 (= S114), S143 (= S115), S144 (= S116), Y170 (= Y140), K174 (= K144), Y197 (= Y167), A200 (≠ V170)
- binding uridine-5'-diphosphate: N199 (= N169), H218 (= H188), L219 (≠ V189), I222 (≠ N192), M241 (≠ F203), P242 (≠ F204), I243 (≠ L205), F244 (= F206), C255 (≠ A217), R257 (= R219), R324 (= R279), D327 (= D282)
8wovB Crystal structure of arabidopsis thaliana udp-glucose 4-epimerase 2 (atuge2) complexed with udp, g233a mutant (see paper)
40% identity, 88% coverage: 2:282/318 of query aligns to 4:300/341 of 8wovB
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), N34 (= N32), D36 (≠ S34), N37 (= N35), S38 (≠ G36), D63 (= D50), L64 (≠ I51), F85 (= F72), A86 (= A73), G87 (≠ A74), K89 (≠ A76), N104 (= N91), S127 (= S114), S129 (= S116), Y153 (= Y140), K157 (= K144), Y181 (= Y167), P184 (≠ V170)
- binding uridine-5'-diphosphate: N183 (= N169), N203 (≠ H188), L204 (≠ V189), L219 (≠ F204), T220 (vs. gap), F222 (= F206), R235 (= R219), Y237 (= Y221), R297 (= R279), D300 (= D282)
7xpqA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glcnac (see paper)
41% identity, 88% coverage: 2:282/318 of query aligns to 3:301/343 of 7xpqA
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), N36 (= N35), D62 (= D50), L63 (≠ I51), F84 (= F72), A85 (= A73), G86 (≠ A74), K88 (≠ A76), N103 (= N91), S126 (= S114), S128 (= S116), Y152 (= Y140), K156 (= K144), Y180 (= Y167), P183 (≠ V170)
- binding uridine-diphosphate-n-acetylglucosamine: N182 (= N169), N202 (≠ H188), L203 (≠ V189), T219 (vs. gap), Y221 (≠ F206), R234 (= R219), Y236 (= Y221), V275 (= V256), R298 (= R279), D301 (= D282)
7xpoA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glc (see paper)
41% identity, 88% coverage: 2:282/318 of query aligns to 3:301/344 of 7xpoA
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), N36 (= N35), D62 (= D50), L63 (≠ I51), F84 (= F72), A85 (= A73), G86 (≠ A74), K88 (≠ A76), N103 (= N91), S126 (= S114), S128 (= S116), Y152 (= Y140), K156 (= K144), Y180 (= Y167), P183 (≠ V170)
- binding uridine-5'-diphosphate-glucose: S128 (= S116), A129 (= A117), F181 (= F168), N182 (= N169), N202 (≠ H188), L203 (≠ V189), T219 (vs. gap), V220 (≠ L205), Y221 (≠ F206), R234 (= R219), Y236 (= Y221), V275 (= V256), R298 (= R279), D301 (= D282)
4lisA Crystal structure of udp-galactose-4-epimerase from aspergillus nidulans (see paper)
40% identity, 92% coverage: 2:295/318 of query aligns to 4:328/364 of 4lisA
- active site: S127 (= S116), A128 (= A117), T129 (≠ A118), Y155 (= Y140), K159 (= K144)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), N34 (= N32), N37 (= N35), S38 (≠ G36), D60 (= D50), V61 (≠ I51), F83 (= F72), A85 (= A74), K87 (≠ A76), S127 (= S116), Y155 (= Y140), K159 (= K144), Y196 (= Y167), P199 (≠ V170)
- binding uridine-5'-diphosphate-glucose: K87 (≠ A76), S127 (= S116), Y155 (= Y140), N218 (≠ V189), L235 (≠ F204), V236 (≠ L205), F237 (= F206), R250 (= R219), R312 (= R279), D315 (= D282)
4lisB Crystal structure of udp-galactose-4-epimerase from aspergillus nidulans (see paper)
40% identity, 92% coverage: 2:295/318 of query aligns to 3:327/363 of 4lisB
- active site: S126 (= S116), A127 (= A117), T128 (≠ A118), Y154 (= Y140), K158 (= K144)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), N36 (= N35), S37 (≠ G36), D59 (= D50), V60 (≠ I51), F82 (= F72), A84 (= A74), K86 (≠ A76), S126 (= S116), Y154 (= Y140), K158 (= K144), Y195 (= Y167), P198 (≠ V170)
- binding uridine-5'-diphosphate: N197 (= N169), N217 (≠ V189), L218 (= L190), L234 (≠ F204), V235 (≠ L205), F236 (= F206), R249 (= R219), V288 (= V256), R311 (= R279), D314 (= D282)
Query Sequence
>WP_073040341.1 NCBI__GCF_900129305.1:WP_073040341.1
MNVLVTGGAGYIGSHTAKLIKGRGHTPVVFDNLSNGWAEWARFGPFVFGDIRDEEALFRT
LQAFRIDAVIHFAALASVEESTRRPGLYFDNNVGGTIALVQAMARAGVRRLVFSSSAAVY
GKARTRPIREDHPREPTNPYGLSKWQCEQVVETVAAAEGIHFAALRYFNVVGNDPDGELY
ERHEPETHVLPNLLRAARSGEPFFLFGTDHDTPDGTAVRDYVYVMDLAEAHLKALDVIRN
QPRLVSNVGRGRGVSVREMVRAVEEVLNVRVNVQERPIRAGDPPELVADNTFLRTWFPRE
FKELPDVIREMAGRPPYA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory