SitesBLAST

P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
41% identity, 96% coverage: 8:394/405 of query aligns to 12:397/405 of P40875

query
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P40875

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G30 (= G26) mutation to A: Prevents covalent attachment of FMN. Blocks subunit assembly.
C146 (≠ I142) mutation to S: No change in activity.
H173 (= H169) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
H175 (≠ A171) mutation to A: No effect on FMN binding and activity.
C195 (≠ T191) mutation to S: No change in activity.
C351 (= C348) mutation to A: No change in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
40% identity, 96% coverage: 8:394/405 of query aligns to 11:396/402 of 1vrqB

query
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1vrqB

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active site: A62 (≠ G59), T65 (= T62), T66 (≠ A63), G326 (= G323), K358 (= K356)
binding n,n-dimethylglycine: T65 (= T62), I67 (= I64), R69 (= R66), K358 (= K356)
binding flavin-adenine dinucleotide: V26 (= V23), G27 (= G24), G29 (= G26), G30 (≠ L27), H31 (= H28), L51 (= L48), E52 (= E49), K53 (= K50), G59 (= G56), N60 (≠ S57), M61 (≠ A58), R63 (= R60), N64 (= N61), T65 (= T62), I67 (= I64), V196 (= V193), A224 (= A221), G225 (≠ A222), H228 (≠ W225), L247 (≠ I244), G353 (= G351), T354 (≠ Y352), G355 (≠ F353), G356 (= G354), F357 (= F355), K358 (= K356)
binding flavin mononucleotide: A62 (≠ G59), R63 (= R60), H172 (= H169), V251 (= V248), E279 (= E276), R322 (= R319), W324 (= W321)

Sites not aligning to the query:

binding n,n-dimethylglycine: 401

3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
40% identity, 96% coverage: 8:394/405 of query aligns to 11:396/404 of 3ad8B

query
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3ad8B

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active site: A62 (≠ G59), T65 (= T62), T66 (≠ A63), G326 (= G323), K358 (= K356)
binding flavin-adenine dinucleotide: V26 (= V23), G27 (= G24), G29 (= G26), G30 (≠ L27), H31 (= H28), E52 (= E49), K53 (= K50), G59 (= G56), N60 (≠ S57), M61 (≠ A58), R63 (= R60), N64 (= N61), T65 (= T62), I67 (= I64), V196 (= V193), G225 (≠ A222), A226 (≠ G223), H228 (≠ W225), L247 (≠ I244), G353 (= G351), T354 (≠ Y352), G355 (≠ F353), G356 (= G354), F357 (= F355), K358 (= K356)
binding flavin mononucleotide: A62 (≠ G59), R63 (= R60), H172 (= H169), V251 (= V248), E279 (= E276), R322 (= R319), W324 (= W321)
binding pyrrole-2-carboxylate: T65 (= T62), I67 (= I64), R69 (= R66), M264 (≠ S261), Y271 (= Y268), T354 (≠ Y352), K358 (= K356)

Sites not aligning to the query:

binding pyrrole-2-carboxylate: 402

3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
40% identity, 96% coverage: 8:394/405 of query aligns to 11:396/404 of 3ad7B

query
sites
3ad7B

L

L

W

W

K

N

Q

N

A

P

P

E

F

P

R

K

S

K

T

S

Y

Y

D

D

A

V

V

V

I

I

V
|
V

G
|
G

G

G

G
|
G

L
x
G

H
|
H

G

G

L

L

A

A

T

T

A

A

Y

Y

L

L

A

A

K

K

N

N

H

H

G

G

V

I

R

T

R

N

V

V

A

A

V

V

L

L

E
|
E

K
|
K

N

G

H

W

I

L

G

A

Y

G

G
|
G

S
x
N

A
x
M

G
x
A

R
|
R

N
|
N

T
|
T

A
x
T

I
|
I

V

I

R
|
R

A

S

N

N

Q

Y

R

L

T

W

Q

D

E

E

N

S

I

A

P

G

L

I

Y

Y

D

E

E

K

G

S

L

L

K

K

M

L

W

W

P

E

Q

E

L

L

M

P

D

E

E

E

L

L

D

E

F

Y

N

D

L

F

M

L

F

F

F

S

N

Q

C

R

G

G

N

V

L

L

N

N

L

L

A

A

H

H

S

T

E

L

A

G

A

D

M

V

G

R

A

E

L

S

R

I

L

R

Q

R

V

V

A

E

S

A

A

N

Q

K

Y

F

L

N

G

G

V

V

R

D

S

A

E

E

L

W

L

L

D

T

R

P

K

E

Q

Q

C

V

K

K

E

E

L

V

I

C

P

P

H

I

L

I

N

N

I

T

S

G

E

D

D

N

I

I

T

R

Y

Y

P

P

V

V

F

M

G

G

G

A

M

T

Y

Y

H

Q

P

P

P

R

G

A

G

G

T

I

V

A

R

K

H
|
H

D

D

A

H

L

V

V

A

W

W

A

A

L

F

A

A

K

R

G

K

A

A

S

N

A

E

L

M

G

G

V

V

H

D

I

I

H

I

Q

Q

Q

N

T

C

E

E

V
|
V

T

T

G

G

I

F

A

L

V

K

H

D

R

G

G

E

R

K

V

V

T

T

A

G

V

V

E

K

T

T

S

T

R

R

G

G

T

T

I

I

H

L

T

A

L

G

R

K

V

V

L

A

N

L

A

A

A
x
G

G
x
A

P

G

W
x
H

S

S

V

S

L

V

V

L

S

A

A

E

M

L

V

A

D

G

I

F

R

E

L

L

P

P

I

I

H

Q

V

S

L

H

P

P

I
x
L

Q

Q

A

A

M

L

V
|
V

T

S

E

E

P

L

L

F

K

E

P

P

F

V

L

H

H

P

H

T

V

V

I

V

S
x
M

S

S

G

N

A

H

Y

I

H

H

C

V

Y
|
Y

A

V

N

S

Q

Q

S

A

L

H

K
|
K

G

G

E
|
E

V

L

V

V

T

M

G

G

A

A

H

G

M

I

D

D

P

S

W

Y

I

N

S

G

Y

Y

G

G

T

Q

H

R

V

G

T

A

A

F

H

H

Y

V

I

I

K

E

H

E

Q

Q

A

M

E

A

A

A

L

A

T

V

E

E

L

L

L

F

P

P

C

I

L

F

R

A

G

R

V

A

K

H

F

V

M

L

R
|
R

I

T

W
|
W

G

G

G
|
G

L

I

A

V

D

D

M

T

T

T

P

M

D

D

M

A

A

S

P

P

I

I

D

S

G

-

N

K

D

T

P

P

I

I

E

Q

G

N

Y

L

Y

Y

M

V

D

N

C

C

G

G

W

W

G
|
G

Y
x
T

F
x
G

G
|
G

F
|
F

K
|
K

S

G

A

T

A

P

V

G

T

A

G

G

K

Y

Y

T

V

L

A

A

E

H

Y

T

M

I

A

A

T

H

G

D

R

E

C

P

P

H

K

K

I

L

L

N

Q

A

P

P

F

F

R

A

L

L

K

E

R

R

Y

F

E

E

E

T

N

G

R

H

L

L

L

I

G

D

E

E

active site: A62 (≠ G59), T65 (= T62), T66 (≠ A63), G326 (= G323), K358 (= K356)
binding flavin-adenine dinucleotide: V26 (= V23), G27 (= G24), G29 (= G26), G30 (≠ L27), H31 (= H28), E52 (= E49), K53 (= K50), G59 (= G56), N60 (≠ S57), M61 (≠ A58), R63 (= R60), N64 (= N61), T65 (= T62), I67 (= I64), V196 (= V193), G225 (≠ A222), A226 (≠ G223), H228 (≠ W225), L247 (≠ I244), G353 (= G351), T354 (≠ Y352), G355 (≠ F353), G356 (= G354), F357 (= F355), K358 (= K356)
binding flavin mononucleotide: A62 (≠ G59), R63 (= R60), H172 (= H169), V251 (= V248), K277 (= K274), E279 (= E276), R322 (= R319), W324 (= W321)
binding [methylthio]acetate: I67 (= I64), R69 (= R66), M264 (≠ S261), Y271 (= Y268), T354 (≠ Y352), K358 (= K356)

Sites not aligning to the query:

binding [methylthio]acetate: 402

Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
40% identity, 96% coverage: 8:394/405 of query aligns to 12:397/405 of Q50LF2

query
sites
Q50LF2

L

L

W

W

K

N

Q

N

A

P

P

E

F

P

R

K

S

K

T

S

Y

Y

D

D

A

V

V

V

I

I

V

V

G

G

L
x
G

H
|
H

G

G

L

L

A

A

T

T

A

A

Y

Y

L

L

A

A

K

K

N

N

H

H

G

G

V

I

R

T

R

N

V

V

A

A

V

V

L

L

E
|
E

K

K

N

G

H

W

I

L

G

A

Y

G

G

G

S
x
N

A
x
M

G

A

R

R

N

N

T
|
T

A

T

I
|
I

V

I

R

R

A

S

N

N

Q

Y

R

L

T

W

Q

D

E

E

N

S

I

A

P

G

L

I

Y

Y

D

E

E

K

G

S

L

L

K

K

M

L

W

W

P

E

Q

E

L

L

M

P

D

E

E

E

L

L

D

E

F

Y

N

D

L

F

M

L

F

F

F

S

N

Q

C

R

G

G

N

V

L

L

N

N

L

L

A

A

H

H

S

T

E

L

A

G

A

D

M

V

G

R

A

E

L

S

R

I

L

R

Q

R

V

V

A

E

S

A

A

N

Q

K

Y

F

L

N

G

G

V

V

R

D

S

A

E

E

L

W

L

L

D

T

R

P

K

E

Q

Q

C

V

K

K

E

E

L

V

I

C

P

P

H

I

L

I

N

N

I

T

S

G

E

D

D

N

I

I

T

R

Y

Y

P

P

V

V

F

M

G

G

G

A

M

T

Y

Y

H

Q

P

P

P

R

G

A

G

G

T

I

V

A

R
x
K

H
|
H

D

D

A

H

L

V

V

A

W

W

A

A

L

F

A

A

K

R

G

K

A

A

S

N

A

E

L

M

G

G

V

V

H

D

I

I

H

I

Q

Q

Q

N

T

C

E

E

V
|
V

T

T

G

G

I

F

A

L

V

K

H

D

R

G

G

E

R

K

V

V

T

T

A

G

V

V

E

K

T

T

S

T

R

R

G

G

T

T

I

I

H

L

T

A

L

G

R

K

V

V

L

A

N

L

A

A

A

G

G

A

P

G

W

H

S

S

V

S

L

V

V

L

S

A

A

E

M

L

V

A

D

G

I

F

R

E

L

L

P

P

I

I

H

Q

V

S

L

H

P

P

I

L

Q

Q

A

A

M

L

V

V

T

S

E

E

P

L

L

F

K

E

P

P

F

V

L

H

H

P

H

T

V

V

I

V

S

M

S

S

G

N

A

H

Y

I

H
|
H

C

V

Y
|
Y

A

V

N

S

Q

Q

S

A

L

H

K

K

G

G

E

E

V

L

V

V

T

M

G

G

A

A

H

G

M

I

D

D

P

S

W

Y

I

N

S

G

Y

Y

G

G

T

Q

H

R

V

G

T

A

A

F

H

H

Y

V

I

I

K

E

H

E

Q

Q

A

M

E

A

A

A

L

A

T

V

E

E

L

L

L

F

P

P

C

I

L

F

R

A

G

R

V

A

K

H

F

V

M

L

R

R

I

T

W

W

G

G

L

I

A

V

D

D

M

T

T

T

P

M

D

D

M

A

A

S

P

P

I

I

D

S

G

-

N

K

D

T

P

P

I

I

E

Q

G

N

Y

L

Y

Y

M

V

D

N

C

C

G

G

W

W

G
|
G

Y

T

F

G

G
|
G

F

F

K
|
K

S

G

A

T

A

P

V

G

T

A

G

G

K

Y

Y

T

V

L

A

A

E

H

Y

T

M

I

A

A

T

H

G

D

R

E

C

P

P

H

K

K

I

L

L

N

Q

A

P

P

F

F

R

A

L

L

K

E

R

R

Y

F

E

E

E

T

N

G

R

H

L

L

L

I

G

D

E

E

G31 (≠ L27) binding FAD
H32 (= H28) binding FAD
E53 (= E49) binding FAD
N61 (≠ S57) binding FAD
M62 (≠ A58) binding FAD
T66 (= T62) binding FAD
I68 (= I64) binding FAD
K172 (≠ R168) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
H173 (= H169) modified: Tele-8alpha-FMN histidine
V197 (= V193) binding FAD
H270 (= H266) mutation to A: 10-fold decrease in catalytic efficiency.
Y272 (= Y268) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
G354 (= G351) binding FAD
G357 (= G354) binding FAD
K359 (= K356) binding FAD; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
27% identity, 85% coverage: 43:387/405 of query aligns to 28:372/374 of 1y56B

query
sites
1y56B

R

E

V

V

A

T

V

V

L
x
I

E
|
E

K
|
K

N

R

H

F

I

I

G

G

Y

S

G

G

S
|
S

A
x
T

G
x
F

R
|
R

N
x
C

T
x
G

A
x
T

I
x
G

V

I

R

R

A

Q

N

Q

Q

F

R

N

T

D

Q

E

E

A

N

N

I

V

P

R

L

V

Y

M

D

K

E

R

G

S

L

V

K

E

M

L

W

W

P

K

Q

K

L

Y

M

S

D

E

E

E

L

Y

D

G

F

F

N

S

L

-

M

-

F

F

F

K

N

Q

C

T

G

G

N

Y

L

L

N

F

L

L

A

L

H

Y

S

D

E

D

A

E

M

V

G

K

A

T

L

F

R

K

L

R

Q

N

V

I

A

E

S

I

A

Q

Q

N

Y

K

L

F

G

G

V

V

R

P

S

T

E

K

L

L

L

I

D

T

R

P

K

E

Q

E

C

A

K

K

E

E

L

I

I

V

P

P

H

L

L

L

N

D

I

I

S

S

E

E

D

-

I

-

T

-

Y

-

P

-

V

V

F

I

G

A

M

S

Y

W

H

N

P

P

P

T

G

D

G

G

T

K

V

A

R

D

H

P

D

F

A

E

L

A

V

T

W

T

A

A

L

F

A

A

K

V

G

K

A

A

S

K

A

E

L

Y

G

G

V

A

H

K

I

L

H

L

Q

E

Q

Y

T

T

E
|
E

V
|
V

T

K

G

G

I

F

A

L

V

I

H

E

R

N

G

N

R

E

V

I

T

K

A

G

V

V

E

K

T

T

S

N

R

K

G

G

T

I

I

I

H

K

T

T

L

G

R

I

V

V

L

V

N

N

A

A

A
x
T

G
x
N

P

A

W
|
W

S

A

V

N

L

L

V

I

S

N

A

A

M

M

V

A

D

G

I

I

R

K

L

T

P

K

I

I

H

P

V

I

L

E

P

P

I

Y

-

K

-
x
H

Q

Q

A

A

M

V

V

I

T

T

E

Q

P

P

L

I

K

K

-

R

-

G

P

T

F

I

L

N

H
x
P

H

M

V

V

I

I

S

S

S

F

G

K

A

Y

Y

G

H

H

C

A

Y

Y

A

L

N

T

Q

Q

S

T

L

F

K

H

G

G

E

G

V

I

V
x
I

T

G

G

G

A

I

H

G

M

Y

D

E

P

I

W

G

I

P

S

T

Y

Y

G

D

T

L

H

T

V

P

T

T

A

Y

H

E

Y

F

I

L

K

R

H

E

Q

V

A

S

E

Y

A

Y

L

F

T

T

E

K

L

I

P

P

C

A

L

L

R

K

G

N

V

L

K

L

F

I

M

L

R
|
R

I

T

W
|
W

G

A

G
|
G

L
x
Y

A
x
Y

D

A

M

K

T

T

P

P

D

D

M

S

A

N

P

P

I

A

I

I

D

G

G

R

N

I

D

E

P

E

I

L

E

N

G

D

Y

Y

M

I

D

A

C

A

G

G

W

F

G

S

Y
x
G

F
x
H

G
|
G

F
|
F

K
x
M

S

M

A

A

A

P

V

A

T

V

G

G

K

E

Y

M

V

V

A

A

E

E

Y

L

M

I

A

T

T

K

G

G

R

K

C

T

P

K

K

L

I

P

L

V

Q

E

-

W

-

Y

-

D

P

P

F

Y

R

R

L

F

K

E

R

R

Y

G

E

E

active site: F44 (≠ G59), G47 (≠ T62), T48 (≠ A63), H224 (vs. gap), P239 (≠ H257), G305 (= G323), M338 (≠ K356)
binding flavin-adenine dinucleotide: I33 (≠ L48), E34 (= E49), K35 (= K50), S42 (= S57), T43 (≠ A58), R45 (= R60), C46 (≠ N61), G47 (≠ T62), G49 (≠ I64), E170 (= E192), V171 (= V193), T200 (≠ A222), N201 (≠ G223), W203 (= W225), G305 (= G323), Y306 (≠ L324), Y307 (≠ A325), G334 (≠ Y352), H335 (≠ F353), G336 (= G354), F337 (= F355), M338 (≠ K356)
binding flavin mononucleotide: F44 (≠ G59), R45 (= R60), I260 (≠ V278), R301 (= R319), W303 (= W321)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 14, 15

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
24% identity, 92% coverage: 14:386/405 of query aligns to 2:388/824 of 4pabB

query
sites
4pabB

F

W

R

K

S

D

T

R

Y

A

D

E

A

T

V

V

I

I

V
x
I

G
|
G

G

G

G
|
G

L
x
C

H
x
V

G

G

L

V

A

S

T

L

A

A

Y

Y

Y

H

L

L

A

A

K

K

N

A

H

-

G

G

V

M

R

R

R

D

V

V

A

V

V

L

L
|
L

E
|
E

K
|
K

N

S

H

E

I

L

G

T

Y

A

G
|
G

S
|
S

A
x
T

G

W

R
x
H

N
x
A

T
x
A

A
x
G

I
x
L

V

T

R
x
T

A

Y

N

F

Q

H

R

P

T

G

Q

I

E

N

N

L

I

K

P

K

L

I

Y

H

D

Y

E

D

G

S

L

I

K

K

M

L

W

Y

P

E

Q

R

L

L

M

E

D

E

E

E

L

T

D

G

F

Q

N

V

L

V

M

G

F

F

F

H

N

Q

C

P

G

G

N

S

L

I

N

R

L

L

A

A

H

T

S

T

E

P

A

E

A

R

M

V

G

D

A
x
E

L

F

R

K

L

Y

Q

Q

V

M

A

T

S

R

A

T

Q

N

Y

W

L

H

G

A

V

T

R

E

S

Q

E

Y

L

I

D

E

R

P

K

E

Q

K

C

I

K

H

E

E

L

L

I

F

P

P

H

L

L

L

N

N

I

M

S

D

E

K

D

-

I

-

T

-

Y

-

P

-

V

I

F

L

G

A

G

G

M

L

Y

Y

H

N

P

P

P

G

G

D

G

G

T

H

V

I

R

D

H

P

D

Y

A

S

L

L

V

T

W

M

A

A

L

L

A

A

K

T

G

G

A

A

S

R

A

K

L

Y

G

G

V

V

H

L

I

L

H

K

Q

Y

Q

P

T

A

E

P

V
|
V

T

T

G

S

I

L

A

K

V

P

H

R

R

P

G

D

R

G

V

T

T

W

A

D

V

V

E

E

T

T

S

P

R

Q

G

G

T

S

I

V

H

R

T

A

L

N

R

R

V

I

L

V

N

N

A

A

A
|
A

G
|
G

P

F

W
|
W

S

A

V

R

L

E

V

V

S

G

A

K

M

M

V

I

D

G

I

L

R

D

L

H

P

P

I

L

H

I

V

P

L

V

P

Q

I
x
H

Q

Q

A
x
Y

M

V

V

V

T

T

E

S

P

T

L

I

K

P

-

E

-

V

-

K

-

A

-

L

-

K

-

R

-

E

-

L

P

P

F

V

L

L

H

R

H

D

V

L

I

-

S

-

S

E

G

G

A

S

Y

Y

H
x
Y

C

L

Y

R

A

Q

N

E

Q

R

S

D

-

G

-

L

L

L

K

F

G

G

E

P

V

Y

V

E

T

S

G

Q

A

E

H

K

M

M

D

K

-

L

-

Q

-

A

P

S

W

W

I

V

S

A

Y

H

G

G

T

V

-

P

-

G

-

F

-

G

-

K

-

E

-

L

-

F

H

E

V

S

T

D

A

L

H

D

Y

R

I

I

K

T

H

E

Q

H

A

V

E

E

A

A

L

A

T

M

E

E

L

M

L

V

P

P

C

V

L

L

R

K

G

K

V

A

K

D

F

I

M

I

R

N

I

I

W

V

G

N

G
|
G

L

P

A
x
I

D

T

M

Y

T

S

P

P

D

D

M

I

A

L

P

P

I

M

I

V

D

G

G

P

N

H

D

Q

P

G

I

V

E

R

G

N

Y

Y

Y

W

M

V

D

A

C

I

G

G

W
x
F

G

G

Y
|
Y

F

-

G
|
G

F
x
I

K
x
I

S

H

A

A

A

G

V

G

T

V

G

G

K

K

Y

Y

V

L

A

S

E

D

Y

W

M

I

A

L

T

H

G

G

R

E

C

P

P

P

K

F

I

D

L

L

Q

I

P

E

F

L

R

D

L

P

K

N

R

R

Y

Y

active site: T53 (≠ R66), E102 (≠ A115), H226 (≠ I244), Y255 (≠ H266)
binding flavin-adenine dinucleotide: I11 (≠ V23), G12 (= G24), G14 (= G26), C15 (≠ L27), V16 (≠ H28), L35 (= L48), E36 (= E49), K37 (= K50), G43 (= G56), S44 (= S57), T45 (≠ A58), H47 (≠ R60), A48 (≠ N61), A49 (≠ T62), G50 (≠ A63), L51 (≠ I64), V175 (= V193), A204 (= A222), G205 (= G223), W207 (= W225), H226 (≠ I244), Y228 (≠ A246), G326 (= G323), I328 (≠ A325), F353 (≠ W350), Y355 (= Y352), G356 (= G354), I357 (≠ F355), I358 (≠ K356)

Sites not aligning to the query:

active site: 536
binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 92% coverage: 14:386/405 of query aligns to 39:425/857 of Q63342

query
sites
Q63342

F

W

R

K

S

D

T

R

Y

A

D

E

A

T

V

V

I

I

V

I

G

G

L
x
C

H
x
V

G

G

L

V

A

S

T

L

A

A

Y

Y

Y

H

L

L

A

A

K

K

N

A

H

-

G

G

V

M

R

R

R

D

V

V

A

V

V

L

L

L

E
|
E

K
|
K

N

S

H

E

I

L

G

T

Y

A

G
|
G

S
|
S

A
x
T

G
x
W

R
x
H

N
x
A

T
x
A

A
x
G

I
x
L

V

T

R

T

A

Y

N

F

Q

H

R

P

T

G

Q

I

E

N

N

L

I

K

P

K

L

I

Y

H

D

Y

E

D

G

S

L

I

K

K

M

L

W

Y

P

E

Q

R

L

L

M

E

D

E

E

E

L

T

D

G

F

Q

N

V

L

V

M

G

F

F

F

H

N

Q

C

P

G

G

N

S

L

I

N

R

L

L

A

A

H

T

S

T

E

P

A

E

A

R

M

V

G

D

A

E

L

F

R

K

L

Y

Q

Q

V

M

A

T

S

R

A

T

Q

N

Y

W

L

H

G

A

V

T

R

E

S

Q

E

Y

L

I

D

E

R

P

K

E

Q

K

C

I

K

H

E

E

L

L

I

F

P

P

H

L

L

L

N

N

I

M

S

D

E

K

D

-

I

-

T

-

Y

-

P

-

V

I

F

L

G

A

G

G

M

L

Y

Y

H

N

P

P

P

G

G

D

G

G

T

H

V

I

R

D

H

P

D

Y

A

S

L

L

V

T

W

M

A

A

L

L

A

A

K

T

G

G

A

A

S

R

A

K

L

Y

G

G

V

V

H

L

I

L

H

K

Q

Y

Q

P

T

A

E

P

V
|
V

T

T

G

S

I

L

A

K

V

P

H

R

R

P

G

D

R

G

V

T

T

W

A

D

V

V

E

E

T

T

S

P

R

Q

G

G

T

S

I

V

H

R

T

A

L

N

R

R

V

I

L

V

N

N

A

A

G

G

P

F

W
|
W

S

A

V

R

L

E

V

V

S

G

A

K

M

M

V

I

D

G

I

L

R

D

L

H

P

P

I

L

H

I

V

P

L

V

P

Q

I

H

Q

Q

A

Y

M

V

V

V

T

T

E

S

P

T

L

I

K

P

-

E

-

V

-

K

-

A

-

L

-

K

-

R

-

E

-

L

P

P

F

V

L

L

H

R

H

D

V

L

I

-

S

-

S

E

G

G

A

S

Y

Y

H

Y

C

L

Y

R

A

Q

N

E

Q

R

S

D

-

G

-

L

L

L

K

F

G

G

E

P

V

Y

V

E

T

S

G

Q

A

E

H

K

M

M

D

K

-

L

-

Q

-

A

P

S

W

W

I

V

S

A

Y

H

G

G

T

V

-

P

-

G

-

F

-

G

-

K

-

E

-

L

-

F

H

E

V

S

T

D

A

L

H

D

Y

R

I

I

K

T

H

E

Q

H

A

V

E

E

A

A

L

A

T

M

E

E

L

M

L

V

P

P

C

V

L

L

R

K

G

K

V

A

K

D

F

I

M

I

R

N

I

I

W

V

G

N

G

G

L

P

A

I

D

T

M

Y

T

S

P

P

D

D

M

I

A

L

P

P

I

M

I

V

D

G

G

P

N

H

D

Q

P

G

I

V

E

R

G

N

Y

Y

Y

W

M

V

D

A

C

I

G

G

W
x
F

G
|
G

Y
|
Y

F

-

G
|
G

F
x
I

K
x
I

S

H

A

A

A

G

V

G

T

V

G

G

K

K

Y

Y

V

L

A

S

E

D

Y

W

M

I

A

L

T

H

G

G

R

E

C

P

P

P

K

F

I

D

L

L

Q

I

P

E

F

L

R

D

L

P

K

N

R

R

Y

Y

CV 52:53 (≠ LH 27:28) binding FAD
EK 73:74 (= EK 49:50) binding FAD
80:88 (vs. 56:64, 22% identical) binding FAD
H84 (≠ R60) modified: Tele-8alpha-FAD histidine
V212 (= V193) binding FAD
W244 (= W225) binding FAD
FGY-GII 390:395 (≠ WGYFGFK 350:356) binding FAD

Sites not aligning to the query:

573:575 binding (6S)-5,6,7,8-tetrahydrofolate
669 binding (6S)-5,6,7,8-tetrahydrofolate
676:678 binding (6S)-5,6,7,8-tetrahydrofolate
737 binding (6S)-5,6,7,8-tetrahydrofolate

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
24% identity, 94% coverage: 8:386/405 of query aligns to 40:432/866 of Q9UI17

query
sites
Q9UI17

L

L

W

S

K

A

Q

E

A

T

P

Q

F

W

R

K

S

D

T

R

Y

A

D

E

A

T

V

V

I

I

V

I

G

G

L
x
C

H
x
V

G

G

L

V

A

S

T

L

A

A

Y

Y

Y

H

L

L

A

A

K

K

N

-

H

A

G

G

V

M

R

K

R

D

V

V

A

V

V

L

L

L

E
|
E

K
|
K

N

S

H

E

I

L

G

T

Y

A

G
|
G

S
|
S

A
x
T

G
x
W

R
x
H

N
x
A

T
x
A

A
x
G

I
x
L

V

T

R

T

A

Y

N

F

Q

H

R

P

T

G

Q

I

E

N

N

L

I

K

P

K

L

I

Y
x
H

D

Y

E

D

G

S

L

I

K

K

M

L

W

Y

P

E

Q

K

L

L

M

E

D

E

E

E

L

T

D

G

F

Q

N

V

L

V

M

G

F

F

F

H

N

Q

C

P

G

G

N

S

L

I

N

R

L

L

A

A

H

T

S

T

E

P

A

V

A

R

M

V

G

D

A

E

L

F

R

K

L

Y

Q

Q

V

M

A

T

S

R

A

T

Q

G

Y

W

L

H

G

A

V

T

R

E

S

Q

E

Y

L

L

L

I

D

E

R

P

K

E

Q

K

C

I

K

Q

E

E

L

M

I

F

P

P

H

L

L

L

N

N

I

M

S

N

E

K

D

-

I

-

T

-

Y

-

P

-

V

V

F

L

G

A

G

G

M

L

Y

Y

H

N

P

P

P

G

G

D

G

G

T

H

V

I

R

D

H

P

D

Y

A

S

L

L

V

T

W

M

A

A

L

L

A

A

K

A

G

G

A

A

S

R

A

K

L

C

G

G

V

A

H

L

I

L

H

K

Q

Y

Q

P

T

A

E

P

V
|
V

T

T

G

S

I

L

A

K

V

A

H

R

R

S

G

D

R

G

V

T

T

W

A

D

V

V

E

E

T

T

S

P

R

Q

G

G

T

S

I

M

H

R

T

A

L

N

R

R

V

I

L

V

N

N

A

A

G

G

P

F

W

W

S

A

V

R

L

E

V

V

S

G

A

K

M

M

V

I

D

G

I

L

R

E

L

H

P

P

I

L

H

I

V

P

L

V

P

Q

I

H

Q

Q

A

Y

M

V

V

V

T

T

E

S

P

T

L

I

K
x
S

-

E

-

V

-

K

-

A

-

L

-

K

-

R

-

E

-

L

P

P

F

V

L

L

H

R

H

D

V

L

I

-

S

-

S

E

G

G

A

S

Y

Y

H

Y

C

L

Y

R

A

Q

N

E

Q

R

S

D

-

G

-

L

L

L

K

F

G

G

E

P

V

Y

V

E

T

S

G

Q

A

E

H

K

M

M

-

K

-

V

-

Q

D

D

P

S

W

W

I

V

S

T

Y

N

G

G

T

V

-

P

-

G

-

F

-

G

-

K

-

E

-

L

-

F

H

E

V

S

T

D

A

L

H

D

Y

R

I

I

K

M

H

E

Q

H

A

I

E

K

A

A

L

A

T

M

E

E

L

M

L

V

P

P

C

V

L

L

R

K

G

K

V

A

K

D

F

I

M

I

R

N

I

V

W

V

G

N

G

G

L

P

A

I

D

T

M

Y

T

S

P

P

D

D

M

I

A

L

P

P

I

M

I

V

D

G

G

P

N

H

D

Q

P

G

I

V

E

R

G

N

Y

Y

Y

W

M

V

D

A

C

I

G

G

W
x
F

G
|
G

Y
|
Y

F

-

G
|
G

F
x
I

K
x
I

S

H

A

A

A

G

V

G

T

V

G

G

K

K

Y

Y

V

L

A

S

E

D

Y

W

M

I

A

L

T

H

G

G

R

E

C

P

P

P

K

F

I

D

L

L

Q

I

P

E

F

L

R

D

L

P

K

N

R

R

Y

Y

CV 59:60 (≠ LH 27:28) binding FAD
EK 80:81 (= EK 49:50) binding FAD
87:95 (vs. 56:64, 22% identical) binding FAD
H91 (≠ R60) modified: Tele-8alpha-FAD histidine
H109 (≠ Y78) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V193) binding FAD
S279 (≠ K253) to P: in dbSNP:rs532964
FGY-GII 397:402 (≠ WGYFGFK 350:356) binding FAD

Sites not aligning to the query:

530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
25% identity, 94% coverage: 16:397/405 of query aligns to 3:404/830 of Q9AGP8

query
sites
Q9AGP8

S

S

T

T

Y

P

D

R

A

I

V

V

I

I

V

I

G

G

G

A

G

G

L
x
I

H
x
V

G

G

L

T

A

N

T

L

A

A

Y

D

Y

E

L

L

A

V

K

-

N

T

H

R

G

G

V

W

R

N

V

I

A

T

V

V

L

L

E
x
D

K
x
Q

N

G

H

P

I

L

G

N

Y

M

-

P

-

G

G

G

S
|
S

A
x
T

G
x
S

R
x
H

N

A

T

P

A

G

I
x
L

V

V

R

F

A

Q

N

T

Q

N

R

P

T

S

Q

K

E

T

N

M

I

A

P

S

L

F

Y

A

D

K

E

Y

G

T

L

V

K

E

M

K

W

L

P

L

Q

S

L

L

M

T

D

E

E

-

L

-

D

D

F

G

N

V

L

S

M

C

F

F

F

N

N

Q

C

V

G

G

N

G

L

L

N

E

L

V

A

A

H

T

S

T

E

E

A

T

A

R

M

L

G

A

A

D

L

L

R

K

L

R

Q

K

V

L

A

G

S

Y

A

A

Q

A

Y

A

L

W

G

G

V

I

R

E

S

G

E

R

L

L

D

S

R

P

K

A

Q

E

C

C

K

Q

E

E

L

L

I

Y

P

P

H

L

L

L

N

D

I

-

S

G

E

E

D

N

I

I

T

-

Y

-

P

-

V

-

F

L

G

G

M

L

Y

H

H

V

P

P

P

S

G

D

G

G

T

L

V

A

R

S

H

A

D

A

A

R

L

A

V

V

W

Q

A

L

L

L

A

I

K

K

G

R

A

T

S

E

A

S

L

A

G

G

V

V

H

T

I

Y

H

R

Q

G

Q

S

T

T

E

T

V
|
V

T

T

G

G

I

I

A

E

V

Q

H

S

R

G

G

G

R

R

V

V

T

T

A

G

V

V

E

Q

T

T

S

A

R

D

G

G

T

V

I

I

H

P

T

A

L

D

R

I

V

V

L

V

N

S

A

C

A

A

G

G

P

F

W

W

S

G

V

A

L

K

V

I

S

G

A

A

M

M

V

I

D

G

I

M

R

A

L

V

P

P

I

L

H

L

V

P

L

L

P

A

I
x
H

Q

Q

A

Y

M

V

V

K

T

T

E

T

P

P

L

V

K

P

-

A

-

Q

-

G

-

R

-

N

-

D

-

Q

-

P

-

N

-

G

-

A

-

R

-

L

P

P

F

I

L

L

H

R

H

H

V

Q

I

D

S

Q

S

D

G

L