SitesBLAST
Comparing WP_074200562.1 NCBI__GCF_900141795.1:WP_074200562.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
58% identity, 98% coverage: 3:423/428 of query aligns to 5:422/429 of 6ed7A
- active site: Y17 (= Y15), Y144 (= Y143), D245 (= D246), K274 (= K275)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (= Y15), W52 (= W50), W52 (= W50), Y144 (= Y143), D147 (= D146), A217 (= A218), K274 (= K275), R391 (= R392), F393 (= F394), F393 (= F394)
- binding pyridoxal-5'-phosphate: G112 (= G110), S113 (= S111), Y144 (= Y143), H145 (= H144), D245 (= D246), I247 (= I248), K274 (= K275)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
58% identity, 98% coverage: 3:423/428 of query aligns to 5:422/429 of P12995
- Y17 (= Y15) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W50) binding substrate
- GS 112:113 (= GS 110:111) binding pyridoxal 5'-phosphate
- Y144 (= Y143) mutation to F: Severely reduces the aminotransferase activity.
- D147 (= D146) mutation to N: Loss of aminotransferase activity.
- D245 (= D246) binding pyridoxal 5'-phosphate
- R253 (= R254) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K275) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (= G308) binding substrate
- PT 308:309 (= PT 309:310) binding pyridoxal 5'-phosphate
- R391 (= R392) binding substrate; mutation to A: Reduces aminotransferase activity.
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
57% identity, 98% coverage: 3:423/428 of query aligns to 5:422/429 of 1dtyA
- active site: Y17 (= Y15), Y144 (= Y143), E211 (= E212), D245 (= D246), A248 (= A249), K274 (= K275), Y398 (= Y399)
- binding pyridoxal-5'-phosphate: G112 (= G110), S113 (= S111), Y144 (= Y143), H145 (= H144), D245 (= D246), I247 (= I248), K274 (= K275)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
58% identity, 98% coverage: 3:423/428 of query aligns to 5:421/427 of 1mlzA
- active site: Y17 (= Y15), Y144 (= Y143), E210 (= E212), D244 (= D246), A247 (= A249), K273 (= K275), Y397 (= Y399)
- binding pyridoxal-5'-phosphate: G112 (= G110), S113 (= S111), Y144 (= Y143), H145 (= H144), D244 (= D246), I246 (= I248), K273 (= K275), P307 (= P309), T308 (= T310)
- binding trans-amiclenomycin: W52 (= W50), W53 (= W51), Y144 (= Y143), K273 (= K275), R390 (= R392), F392 (= F394)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
58% identity, 98% coverage: 3:423/428 of query aligns to 5:421/427 of 1mlyA
- active site: Y17 (= Y15), Y144 (= Y143), E210 (= E212), D244 (= D246), A247 (= A249), K273 (= K275), Y397 (= Y399)
- binding cis-amiclenomycin: W52 (= W50), W53 (= W51), K273 (= K275), R390 (= R392), F392 (= F394)
- binding pyridoxal-5'-phosphate: G112 (= G110), S113 (= S111), Y144 (= Y143), H145 (= H144), D244 (= D246), I246 (= I248), K273 (= K275), P307 (= P309), T308 (= T310)
6erkA Crystal structure of diaminopelargonic acid aminotransferase from psychrobacter cryohalolentis (see paper)
52% identity, 98% coverage: 6:424/428 of query aligns to 9:415/420 of 6erkA
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
57% identity, 98% coverage: 3:423/428 of query aligns to 5:410/416 of 1qj3A
- active site: Y17 (= Y15), Y144 (= Y143), E201 (= E212), D235 (= D246), A238 (= A249), K264 (= K275), Y386 (= Y399)
- binding 7-keto-8-aminopelargonic acid: Y17 (= Y15), W52 (= W50), Y144 (= Y143), K264 (= K275), R379 (= R392), F381 (= F394)
- binding pyridoxal-5'-phosphate: G112 (= G110), S113 (= S111), Y144 (= Y143), H145 (= H144), G146 (= G145), D235 (= D246), I237 (= I248), A238 (= A249), K264 (= K275)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
52% identity, 99% coverage: 4:425/428 of query aligns to 7:425/427 of 4cxqA
- active site: Y18 (= Y15), Y149 (= Y143), E212 (= E212), D246 (= D246), A249 (= A249), K275 (= K275), Y399 (= Y399)
- binding 7-keto-8-aminopelargonic acid: W56 (= W50), Y149 (= Y143), G308 (= G308), T310 (= T310), R392 (= R392)
- binding pyridoxal-5'-phosphate: G116 (= G110), S117 (= S111), Y149 (= Y143), H150 (= H144), G151 (= G145), E212 (= E212), D246 (= D246), I248 (= I248), K275 (= K275), P309 (= P309), T310 (= T310)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
52% identity, 99% coverage: 4:425/428 of query aligns to 7:423/425 of 4w1vA
- active site: Y18 (= Y15), Y147 (= Y143), E210 (= E212), D244 (= D246), A247 (= A249), K273 (= K275), Y397 (= Y399)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P14), Y18 (= Y15), W54 (= W50), M81 (= M77), G83 (= G79), Y147 (= Y143), G306 (= G308), P307 (= P309), T308 (= T310), F392 (= F394)
- binding pyridoxal-5'-phosphate: G114 (= G110), S115 (= S111), Y147 (= Y143), H148 (= H144), E210 (= E212), D244 (= D246), I246 (= I248), K273 (= K275)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
52% identity, 99% coverage: 4:425/428 of query aligns to 7:423/425 of 4cxrA
- active site: Y18 (= Y15), Y147 (= Y143), E210 (= E212), D244 (= D246), A247 (= A249), K273 (= K275), Y397 (= Y399)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (= Y15), W54 (= W50), W55 (= W51), A216 (= A218)
- binding pyridoxal-5'-phosphate: G114 (= G110), S115 (= S111), Y147 (= Y143), H148 (= H144), E210 (= E212), D244 (= D246), I246 (= I248), K273 (= K275), P307 (= P309), T308 (= T310)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
52% identity, 99% coverage: 4:425/428 of query aligns to 14:433/437 of P9WQ81